CN103355179A - Biological environment-friendly zymogen bed and preparation method thereof - Google Patents
Biological environment-friendly zymogen bed and preparation method thereof Download PDFInfo
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- CN103355179A CN103355179A CN2013101504631A CN201310150463A CN103355179A CN 103355179 A CN103355179 A CN 103355179A CN 2013101504631 A CN2013101504631 A CN 2013101504631A CN 201310150463 A CN201310150463 A CN 201310150463A CN 103355179 A CN103355179 A CN 103355179A
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Abstract
The invention discloses a biological environment-friendly zymogen bed and a preparation method thereof. The zymogen bed comprises pigpen feces, plant residual wastes and biological zymogen agents. The preparation method comprises the steps of preparation of the biological zymogen agents, mixing of prepared materials, and heap fermentation, wherein a weight ratio of the pigpen feces to the plant residual wastes to the biological zymogen agents is (300-500):(200-400):(1-3). The zymogen bed and the preparation method have the advantages that the pigpen feces are treated; wastes are changed into valuables; the problem of pollution of the feces to an environment is solved; the preparation method is simple; the treatment cost is low; an effect is good; and economic benefits are generated.
Description
Technical field
The present invention relates to a kind of zymophyte bed, specifically a kind of biological environmental production zymophyte bed and preparation method thereof belongs to zymophyte bed field.
Background technology
Along with the developing rapidly of scale animal and poultry cultivation industry, environment has been caused serious pollution in recent years.The year of whole nation feces of livestock and poultry produces total amount about 3,000,000,000 tons, has surpassed the output of industrial solid wastes, and cultivation ight soil enters water body flowing water rate up to 25～30%, and in some areas, livestock and poultry breeding industry becomes the pollution source of local water body maximum just gradually.At present, the dirty treatment technology of various cultivation excrement is confined to absorb urban sewage or technology of garbage disposal more, and one-time investment is large, and cost is high, and operation is complicated, and effect is also unsatisfactory.
Summary of the invention
In order to address the above problem, the present invention has designed a kind of biological environmental production zymophyte bed and preparation method thereof, and piggery feces is processed, turn waste into wealth, solved the pollution of ight soil to environment, the preparation method is simple, processing cost is low, and is effective, produced economic benefit.
Technical scheme of the present invention is:
A kind of biological environmental production zymophyte bed, described zymophyte bed comprises piggery feces, plant leftover and biofermentation microbial inoculum; The weight ratio of described piggery feces, plant leftover and biofermentation microbial inoculum is 300～500: 200～400: 1～3;
The water content of described piggery feces is that 40～60%, pH value is 6.5～7.5;
Described plant leftover is one or more of crops straw, rice husk, wheat bran, wood chip etc., and C/N ratio is 40～50: 1;
Described biofermentation microbial inoculum is comprised of saccharomyces cerevisiae (Sac-charomyces cerevisiae) bacterium liquid, bacillus subtilis (Bacillus subtilis) bacterium liquid, De Shi lactic acid bacteria (L.Delbrueckii) bacterium liquid, A Nandeshi streptomycete (Streptomyces anandii) bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacteria liquid are long-pending than being 2～3: 3～4: 3～4: 4～5;
Described biofermentation microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
The preparation method of above-mentioned biological environmental production zymophyte bed may further comprise the steps:
(1) preparation of biofermentation microbial inoculum
Prepare respectively saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required medium is yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000ml; Cultivation temperature is 28～38 ℃; Aerobic fermentation 24～48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described bacillus subtilis bacterium liquid is: required medium is peptone 15.0g, beef extract 0.50g, glucose 20.0g, sodium chloride 5.0g, distilled water 1000ml; Cultivation temperature is 30～36 ℃; Aerobic fermentation 48～72h obtains bacillus subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required medium is brewer's wort 70mL, beef extract 10g, yeast extract 5g, soyabean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required medium is maltose 1g, glucose 1g, sucrose 1g, soluble starch 3g, peptone 2g, beef extract 10g, yeast extract 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains A Nandeshi streptomycete bacterium liquid;
According to above-mentioned volume ratio dog's-nose saccharomycete bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, obtain described biofermentation microbial inoculum;
(2) mixing of getting the raw materials ready
Prepare water content and be 40～60%, the pH value is 6.5～7.5 piggery feces, and C/N ratio is 50: 1 plant leftover, such as one or more mixing of crops straw, rice husk, wheat bran, wood chip etc., according to above-mentioned weight ratio with piggery feces, plant leftover and biofermentation microbial inoculum;
(3) heap fermentation
Above-mentioned mixed fermentation raw material is carried out heap fermentation, keep 40～50 ℃ of fermentation temperatures, can finish fermentation through 7～10 days, obtain the zymophyte bed.
The invention has the advantages that: piggery feces is processed, turned waste into wealth, solved the pollution of ight soil to environment, the preparation method is simple, and processing cost is low, and is effective, produced economic benefit.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for description and interpretation the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of biological environmental production zymophyte bed, described zymophyte bed comprises piggery feces, plant leftover and biofermentation microbial inoculum; The weight ratio of described piggery feces, plant leftover and biofermentation microbial inoculum is 300～500: 200～400: 1～3;
The water content of described piggery feces is that 40～60%, pH value is 6.5～7.5;
Described plant leftover is one or more of crops straw, rice husk, wheat bran, wood chip etc., and C/N ratio is 50: 1;
Described biofermentation microbial inoculum is comprised of saccharomyces cerevisiae (Sac-charomyces cerevisiae) bacterium liquid, bacillus subtilis (Bacillus subtilis) bacterium liquid, De Shi lactic acid bacteria (L.Delbrueckii) bacterium liquid, A Nandeshi streptomycete (Streptomyces anandii) bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacteria liquid are long-pending than being 2～3: 3～4: 3～4: 4～5;
Described biofermentation microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
The preparation method of above-mentioned biological environmental production zymophyte bed may further comprise the steps:
(1) preparation of biofermentation microbial inoculum
Prepare respectively saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required medium is yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000ml; Cultivation temperature is 28～38 ℃; Aerobic fermentation 24～48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described bacillus subtilis bacterium liquid is: required medium is peptone 15.0g, beef extract 0.50g, glucose 20.0g, sodium chloride 5.0g, distilled water 1000ml; Cultivation temperature is 30～36 ℃; Aerobic fermentation 48～72h obtains bacillus subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required medium is brewer's wort 70mL, beef extract 10g, yeast extract 5g, soyabean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required medium is maltose 1g, glucose 1g, sucrose 1g, soluble starch 3g, peptone 2g, beef extract 10g, yeast extract 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains A Nandeshi streptomycete bacterium liquid;
According to above-mentioned volume ratio dog's-nose saccharomycete bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, obtain described biofermentation microbial inoculum;
(2) mixing of getting the raw materials ready
Prepare water content and be 40～60%, the pH value is 6.5～7.5 piggery feces, and C/N ratio is 50: 1 plant leftover, such as one or more mixing of crops straw, rice husk, wheat bran, wood chip etc., according to above-mentioned weight ratio with piggery feces, plant leftover and biofermentation microbial inoculum;
(3) heap fermentation
Above-mentioned mixed fermentation raw material is carried out heap fermentation, keep 40～50 ℃ of fermentation temperatures, can finish fermentation through 7～10 days, obtain the zymophyte bed.
Except as otherwise noted, the percentage that adopts among the present invention is percetage by weight.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment the present invention is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. biological environmental production zymophyte bed, it is characterized in that: described zymophyte bed comprises piggery feces, plant leftover and biofermentation microbial inoculum; The weight ratio of described piggery feces, plant leftover and biofermentation microbial inoculum is 300～500: 200～400: 1～3;
The water content of described piggery feces is that 40～60%, pH value is 6.5～7.5;
Described plant leftover is one or more in crops straw, rice husk, wheat bran, the wood chip, and C/N ratio is 40～50: 1;
Described biofermentation microbial inoculum is comprised of saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacteria liquid are long-pending than being 2～3: 3～4: 3～4: 4～5;
Described biofermentation microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
2. the preparation method of biological environmental production zymophyte bed as claimed in claim 1 is characterized in that, may further comprise the steps:
(1) preparation of biofermentation microbial inoculum
Prepare respectively saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required medium is yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000ml; Cultivation temperature is 28～38 ℃; Aerobic fermentation 24～48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described bacillus subtilis bacterium liquid is: required medium is peptone 15.0g, beef extract 0.50g, glucose 20.0g, sodium chloride 5.0g, distilled water 1000ml; Cultivation temperature is 30～36 ℃; Aerobic fermentation 48～72h obtains bacillus subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required medium is brewer's wort 70mL, beef extract 10g, yeast extract 5g, soyabean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required medium is maltose 1g, glucose 1g, sucrose 1g, soluble starch 3g, peptone 2g, beef extract 10g, yeast extract 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains A Nandeshi streptomycete bacterium liquid;
According to above-mentioned volume ratio dog's-nose saccharomycete bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, obtain described biofermentation microbial inoculum;
(2) mixing of getting the raw materials ready
Prepare water content and be 40～60%, the pH value is 6.5～7.5 piggery feces, and C/N ratio is 50: 1 plant leftover, according to above-mentioned weight ratio with piggery feces, plant leftover and biofermentation microbial inoculum;
(3) heap fermentation
Above-mentioned mixed fermentation raw material is carried out heap fermentation, keep 40～50 ℃ of fermentation temperatures, can finish fermentation through 7～10 days, obtain the zymophyte bed.
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Cited By (1)
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| CN104304042A (en) * | 2014-10-10 | 2015-01-28 | 安徽万利生态园林景观有限公司 | Preparing method of novel and long-service life pig house padding and padding bed of padding |
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Application publication date: 20131023 |