CN103355180A - Livestock breeding zymogen bed and preparation method thereof - Google Patents
Livestock breeding zymogen bed and preparation method thereof Download PDFInfo
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- CN103355180A CN103355180A CN2013101504735A CN201310150473A CN103355180A CN 103355180 A CN103355180 A CN 103355180A CN 2013101504735 A CN2013101504735 A CN 2013101504735A CN 201310150473 A CN201310150473 A CN 201310150473A CN 103355180 A CN103355180 A CN 103355180A
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Abstract
The invention discloses a livestock breeding zymogen bed and a preparation method thereof. The zymogen bed is a packing material arranged in a packing material groove, wherein the packing material consists of plant residual waste and a biological zymogen agent, and the biological zymogen agent accounts for 0.5wt% of the plant residual waste. The preparation method comprises the steps of preparation of the biological zymogen agent, mixing of a prepared material, heap fermentation and the like. The zymogen bed and the preparation method have the advantages that pigpen feces are treated by the zymogen bed; excretas such as the feces and urine can be assimilated and decomposed quickly; the wastes can be changed into valuables; the problem of pollution of the feces to an environment is solved; the preparation method is simple, low in treatment cost and good in effect; and economic benefits are generated.
Description
Technical field
The present invention relates to a kind of zymophyte bed, specifically a kind of livestock and poultry cultivation zymophyte bed and preparation method thereof belongs to zymophyte bed field.
Background technology
Along with the developing rapidly of scale animal and poultry cultivation industry, environment has been caused serious pollution in recent years.The year of whole nation feces of livestock and poultry produces total amount about 3,000,000,000 tons, has surpassed the output of industrial solid wastes, and cultivation ight soil enters water body flowing water rate up to 25～30%, and in some areas, livestock and poultry breeding industry becomes the pollution source of local water body maximum just gradually.At present, the dirty treatment technology of various cultivation excrement is confined to absorb urban sewage or technology of garbage disposal more, and one-time investment is large, and cost is high, and operation is complicated, and effect is also unsatisfactory.
Summary of the invention
In order to address the above problem, the present invention has designed a kind of livestock and poultry cultivation zymophyte bed and preparation method thereof, and piggery feces is processed, turn waste into wealth, solved the pollution of ight soil to environment, the preparation method is simple, processing cost is low, and is effective, produced economic benefit.
Technical scheme of the present invention is:
A kind of livestock and poultry cultivation zymophyte bed, described zymophyte bed is the bedding and padding that are arranged in the bedding and padding groove, described bedding and padding are comprised of plant leftover and biofermentation microbial inoculum; The weight fraction that described biofermentation microbial inoculum accounts for plant leftover is 0.5%;
Described plant leftover is one or more of crops straw, rice husk, wheat bran, wood chip etc., and C/N ratio is 40～50: 1, and water content is 40～60%, the pH value is 6.5～7.5;
Described biofermentation microbial inoculum is comprised of saccharomyces cerevisiae (Sac-charomyces cerevisiae) bacterium liquid, bacillus subtilis (Bacillus subtilis) bacterium liquid, De Shi lactic acid bacteria (L.Delbrueckii) bacterium liquid, A Nandeshi streptomycete (Streptomyces anandii) bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacteria liquid are long-pending than being 2～3: 3～4: 3～4: 4～5;
Described biofermentation microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
The preparation method of above-mentioned livestock and poultry cultivation zymophyte bed may further comprise the steps:
(1) preparation of biofermentation microbial inoculum
Prepare respectively saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required medium is yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000ml; Cultivation temperature is 28～38 ℃; Aerobic fermentation 24～48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described bacillus subtilis bacterium liquid is: required medium is peptone 15.0g, beef extract 0.50g, glucose 20.0g, sodium chloride 5.0g, distilled water 1000ml; Cultivation temperature is 30～36 ℃; Aerobic fermentation 48～72h obtains bacillus subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required medium is brewer's wort 70mL, beef extract 10g, yeast extract 5g, soyabean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required medium is maltose 1g, glucose 1g, sucrose 1g, soluble starch 3g, peptone 2g, beef extract 10g, yeast extract 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains A Nandeshi streptomycete bacterium liquid;
According to above-mentioned volume ratio dog's-nose saccharomycete bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, obtain described biofermentation microbial inoculum;
(2) mixing of getting the raw materials ready
Prepare water content and be 40～60%, the pH value is 6.5～7.5, C/N ratio is 50: 1 plant leftover, such as one or more mixing of crops straw, rice husk, wheat bran, wood chip etc., according to above-mentioned weight fraction with plant leftover and biofermentation microbial inoculum;
(3) heap fermentation
Above-mentioned mixed fermentation raw material is carried out heap fermentation, keep 40～50 ℃ of fermentation temperatures, can finish fermentation through 7～10 days, the bedding and padding after the fermentation are laid in the bedding and padding groove can obtain the zymophyte bed.
The invention has the advantages that: utilize zymophyte bed of the present invention that piggery feces is processed, the excreta such as digest and decompose fecaluria can also be turned waste into wealth fast, solved the pollution of ight soil to environment, the preparation method is simple, and processing cost is low, effective, and produced economic benefit.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein only is used for description and interpretation the present invention, is not intended to limit the present invention.
Embodiment 1
A kind of livestock and poultry cultivation zymophyte bed, described zymophyte bed is the bedding and padding that are arranged in the bedding and padding groove, described bedding and padding are comprised of plant leftover and biofermentation microbial inoculum; The weight fraction that described biofermentation microbial inoculum accounts for plant leftover is 0.5%;
Described plant leftover is one or more of crops straw, rice husk, wheat bran, wood chip etc., and C/N ratio is 50: 1, and water content is 40～60%, the pH value is 6.5～7.5;
Described biofermentation microbial inoculum is comprised of saccharomyces cerevisiae (Sac-charomyces cerevisiae) bacterium liquid, bacillus subtilis (Bacillus subtilis) bacterium liquid, De Shi lactic acid bacteria (L.Delbrueckii) bacterium liquid, A Nandeshi streptomycete (Streptomyces anandii) bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacteria liquid are long-pending than being 2～3: 3～4: 3～4: 4～5;
Described biofermentation microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
The preparation method of above-mentioned livestock and poultry cultivation zymophyte bed may further comprise the steps:
(1) preparation of biofermentation microbial inoculum
Prepare respectively saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required medium is yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000ml; Cultivation temperature is 28～38 ℃; Aerobic fermentation 24～48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described bacillus subtilis bacterium liquid is: required medium is peptone 15.0g, beef extract 0.50g, glucose 20.0g, sodium chloride 5.0g, distilled water 1000ml; Cultivation temperature is 30～36 ℃; Aerobic fermentation 48～72h obtains bacillus subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required medium is brewer's wort 70mL, beef extract 10g, yeast extract 5g, soyabean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required medium is maltose 1g, glucose 1g, sucrose 1g, soluble starch 3g, peptone 2g, beef extract 10g, yeast extract 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains A Nandeshi streptomycete bacterium liquid;
According to above-mentioned volume ratio dog's-nose saccharomycete bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, obtain described biofermentation microbial inoculum;
(2) mixing of getting the raw materials ready
Prepare water content and be 40～60%, the pH value is 6.5～7.5, C/N ratio is 50: 1 plant leftover, such as one or more mixing of crops straw, rice husk, wheat bran, wood chip etc., according to above-mentioned weight fraction with plant leftover and biofermentation microbial inoculum;
(3) heap fermentation
Above-mentioned mixed fermentation raw material is carried out heap fermentation, keep 40～50 ℃ of fermentation temperatures, can finish fermentation through 7～10 days, the bedding and padding after the fermentation are laid in the bedding and padding groove can obtain the zymophyte bed.
Except as otherwise noted, the percentage that adopts among the present invention is percetage by weight.
It should be noted that at last: the above only is the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment the present invention is had been described in detail, for a person skilled in the art, it still can be made amendment to the technical scheme that aforementioned each embodiment puts down in writing, and perhaps part technical characterictic wherein is equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. livestock and poultry cultivation zymophyte bed, it is characterized in that: described zymophyte bed is the bedding and padding that are arranged in the bedding and padding groove, described bedding and padding are comprised of plant leftover and biofermentation microbial inoculum; The weight fraction that described biofermentation microbial inoculum accounts for plant leftover is 0.5%;
Described plant leftover is one or more in crops straw, rice husk, wheat bran, the wood chip, and C/N ratio is 40～50: 1, and water content is 40～60%, the pH value is 6.5～7.5;
Described biofermentation microbial inoculum is comprised of saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid; Described saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacteria liquid are long-pending than being 2～3: 3～4: 3～4: 4～5;
Described biofermentation microbial inoculum is the pure fermentation of single bacterial classification, carries out composite obtaining behind the strain fermentation.
2. the preparation method of livestock and poultry cultivation zymophyte bed as claimed in claim 1 is characterized in that, may further comprise the steps:
(1) preparation of biofermentation microbial inoculum
Prepare respectively saccharomyces cerevisiae bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid;
The preparation method of described saccharomyces cerevisiae bacterium liquid is: required medium is yeast extract 10g, peptone 20g, glucose 20g, distilled water 1000ml; Cultivation temperature is 28～38 ℃; Aerobic fermentation 24～48h obtains saccharomyces cerevisiae bacterium liquid;
The preparation method of described bacillus subtilis bacterium liquid is: required medium is peptone 15.0g, beef extract 0.50g, glucose 20.0g, sodium chloride 5.0g, distilled water 1000ml; Cultivation temperature is 30～36 ℃; Aerobic fermentation 48～72h obtains bacillus subtilis bacterium liquid;
The preparation method of described De Shi lactic acid bacterial liquid is: required medium is brewer's wort 70mL, beef extract 10g, yeast extract 5g, soyabean protein powder 5g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains the De Shi lactic acid bacterial liquid;
The preparation method of described A Nandeshi streptomycete bacterium liquid is: required medium is maltose 1g, glucose 1g, sucrose 1g, soluble starch 3g, peptone 2g, beef extract 10g, yeast extract 5g, soybean cake powder 2g, add water to 1000ml, initial pH value is 6.5; Cultivation temperature is 38～42 ℃; Aerobic fermentation 48～72h obtains A Nandeshi streptomycete bacterium liquid;
According to above-mentioned volume ratio dog's-nose saccharomycete bacterium liquid, bacillus subtilis bacterium liquid, De Shi lactic acid bacterial liquid, A Nandeshi streptomycete bacterium liquid, obtain described biofermentation microbial inoculum;
(2) mixing of getting the raw materials ready
Prepare water content and be 40～60%, the pH value is 6.5～7.5, C/N ratio is 50: 1 plant leftover, for example mixing of one or more in crops straw, rice husk, wheat bran, the wood chip, according to above-mentioned weight fraction with plant leftover and biofermentation microbial inoculum;
(3) heap fermentation
Above-mentioned mixed fermentation raw material is carried out heap fermentation, keep 40～50 ℃ of fermentation temperatures, can finish fermentation through 7～10 days, the bedding and padding after the fermentation are laid in the bedding and padding groove can obtain the zymophyte bed.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766284A (en) * | 2014-02-11 | 2014-05-07 | 山东省农业科学院畜牧兽医研究所 | Method for raising livestock and poultry by using wheat hull and straw fermenting bed |
| CN104304042A (en) * | 2014-10-10 | 2015-01-28 | 安徽万利生态园林景观有限公司 | Preparing method of novel and long-service life pig house padding and padding bed of padding |
| CN104335908A (en) * | 2014-10-10 | 2015-02-11 | 安徽万利生态园林景观有限公司 | Novel pig house pad materials capable of improving meat quality and preparation method of pig house pad bed made of novel pig house pad materials |
| CN104381147A (en) * | 2014-10-10 | 2015-03-04 | 安徽万利生态园林景观有限公司 | Stong granule bedding material and preparation method thereof |
| CN108929150A (en) * | 2018-06-29 | 2018-12-04 | 广州市普淳环保科技有限公司 | It is a kind of that conditioner and preparation method thereof is repaired based on the multifunction soil of decomposed livestock and poultry fermenting bed padding and eucalyptus barks |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416610A (en) * | 2008-06-24 | 2009-04-29 | 李季 | Pigpen fermentation bed and use thereof |
| CN101544960A (en) * | 2009-05-06 | 2009-09-30 | 俞嵘 | A leaven dedicated for fermenting bed of microorganism engineering and fermenting bed pad manufacture method |
| CN101565684A (en) * | 2009-06-04 | 2009-10-28 | 千智伟 | Method for making fermenting bed for ecological pig breeding by using composite strain culture |
| CN101692788A (en) * | 2009-10-10 | 2010-04-14 | 广州市得力农业科技有限公司 | Organic biology padding for efficient livestock and poultry and production method thereof |
| CN101849511A (en) * | 2010-06-02 | 2010-10-06 | 黑龙江洛东生物技术有限公司 | Padding for biological fermentation bed, preparation and application thereof |
| CN101948763A (en) * | 2009-10-13 | 2011-01-19 | 中国农业科学院饲料研究所 | High-efficiency compound microbial inoculum for fermenting beds, and preparation method and application thereof |
| CN101948754A (en) * | 2010-09-06 | 2011-01-19 | 成都和谐生物科技有限公司 | Microbial fermentation bed strain and manufacturing method thereof |
| CN102090345A (en) * | 2010-12-08 | 2011-06-15 | 湖北启明生物工程有限公司 | Ultrathin fermentation bed and used fermentation bed microbial inoculum |
| CN102613251A (en) * | 2012-03-08 | 2012-08-01 | 燕淑海 | Mold inhibitor for fermentation bed and preparation method for mold inhibitor |
| CN102747065A (en) * | 2012-07-30 | 2012-10-24 | 宜春强微生物科技有限公司 | High-efficiency fermenting agent for poultry excrement and fermenting method of fermenting agent |
-
2013
- 2013-04-18 CN CN2013101504735A patent/CN103355180A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101416610A (en) * | 2008-06-24 | 2009-04-29 | 李季 | Pigpen fermentation bed and use thereof |
| CN101544960A (en) * | 2009-05-06 | 2009-09-30 | 俞嵘 | A leaven dedicated for fermenting bed of microorganism engineering and fermenting bed pad manufacture method |
| CN101565684A (en) * | 2009-06-04 | 2009-10-28 | 千智伟 | Method for making fermenting bed for ecological pig breeding by using composite strain culture |
| CN101692788A (en) * | 2009-10-10 | 2010-04-14 | 广州市得力农业科技有限公司 | Organic biology padding for efficient livestock and poultry and production method thereof |
| CN101948763A (en) * | 2009-10-13 | 2011-01-19 | 中国农业科学院饲料研究所 | High-efficiency compound microbial inoculum for fermenting beds, and preparation method and application thereof |
| CN101849511A (en) * | 2010-06-02 | 2010-10-06 | 黑龙江洛东生物技术有限公司 | Padding for biological fermentation bed, preparation and application thereof |
| CN101948754A (en) * | 2010-09-06 | 2011-01-19 | 成都和谐生物科技有限公司 | Microbial fermentation bed strain and manufacturing method thereof |
| CN102090345A (en) * | 2010-12-08 | 2011-06-15 | 湖北启明生物工程有限公司 | Ultrathin fermentation bed and used fermentation bed microbial inoculum |
| CN102613251A (en) * | 2012-03-08 | 2012-08-01 | 燕淑海 | Mold inhibitor for fermentation bed and preparation method for mold inhibitor |
| CN102747065A (en) * | 2012-07-30 | 2012-10-24 | 宜春强微生物科技有限公司 | High-efficiency fermenting agent for poultry excrement and fermenting method of fermenting agent |
Non-Patent Citations (1)
| Title |
|---|
| 中国普通微生物菌种保藏管理中心编: "《菌种目录 第3版 1997》", 30 September 1997 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766284A (en) * | 2014-02-11 | 2014-05-07 | 山东省农业科学院畜牧兽医研究所 | Method for raising livestock and poultry by using wheat hull and straw fermenting bed |
| CN103766284B (en) * | 2014-02-11 | 2015-06-17 | 山东省农业科学院畜牧兽医研究所 | Method for raising livestock and poultry by using wheat hull and straw fermenting bed |
| CN104304042A (en) * | 2014-10-10 | 2015-01-28 | 安徽万利生态园林景观有限公司 | Preparing method of novel and long-service life pig house padding and padding bed of padding |
| CN104335908A (en) * | 2014-10-10 | 2015-02-11 | 安徽万利生态园林景观有限公司 | Novel pig house pad materials capable of improving meat quality and preparation method of pig house pad bed made of novel pig house pad materials |
| CN104381147A (en) * | 2014-10-10 | 2015-03-04 | 安徽万利生态园林景观有限公司 | Stong granule bedding material and preparation method thereof |
| CN108929150A (en) * | 2018-06-29 | 2018-12-04 | 广州市普淳环保科技有限公司 | It is a kind of that conditioner and preparation method thereof is repaired based on the multifunction soil of decomposed livestock and poultry fermenting bed padding and eucalyptus barks |
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