CN103314113B - 残粒样脂蛋白胆甾醇的定量方法及用于其的试剂盒 - Google Patents
残粒样脂蛋白胆甾醇的定量方法及用于其的试剂盒 Download PDFInfo
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- CN103314113B CN103314113B CN201180054103.0A CN201180054103A CN103314113B CN 103314113 B CN103314113 B CN 103314113B CN 201180054103 A CN201180054103 A CN 201180054103A CN 103314113 B CN103314113 B CN 103314113B
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- cholesterol
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- cholesterol esterase
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- 102000004895 Lipoproteins Human genes 0.000 title claims abstract description 42
- 108090001030 Lipoproteins Proteins 0.000 title claims abstract description 42
- 230000003098 cholesteric effect Effects 0.000 title abstract description 22
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 84
- 102000000019 Sterol Esterase Human genes 0.000 claims abstract description 71
- 108010055297 Sterol Esterase Proteins 0.000 claims abstract description 71
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 42
- 108010022197 lipoprotein cholesterol Proteins 0.000 claims abstract description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 71
- 239000000203 mixture Substances 0.000 claims description 61
- 108010089254 Cholesterol oxidase Proteins 0.000 claims description 18
- -1 polyoxyethylene Polymers 0.000 claims description 10
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 8
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- 239000012491 analyte Substances 0.000 claims description 6
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 229920013701 VORANOL™ Polymers 0.000 claims description 4
- 125000005037 alkyl phenyl group Chemical group 0.000 claims description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 74
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- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 16
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- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 14
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- 102000004190 Enzymes Human genes 0.000 description 8
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- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
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- 102000014384 Type C Phospholipases Human genes 0.000 description 2
- 108010079194 Type C Phospholipases Proteins 0.000 description 2
- 125000000217 alkyl group Chemical group 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- BZFBDUQOBQHBSZ-DLCQERRASA-N (3s,8r,9s,10r,13s,14s,17s)-17-[3-(dimethylamino)propyl-methylamino]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1h-cyclopenta[a]phenanthren-3-ol;dihydrochloride Chemical compound Cl.Cl.C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](N(C)CCCN(C)C)[C@@]1(C)CC2 BZFBDUQOBQHBSZ-DLCQERRASA-N 0.000 description 1
- 101710139410 1-phosphatidylinositol phosphodiesterase Proteins 0.000 description 1
- AOTXQRRUWFSXCN-UHFFFAOYSA-N 3-(3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonic acid Chemical class COC1=CC(NCC(O)CS(O)(=O)=O)=CC(OC)=C1 AOTXQRRUWFSXCN-UHFFFAOYSA-N 0.000 description 1
- DYPGTLUYQZIULN-UHFFFAOYSA-N 3-(3-methoxy-5-methylanilino)propane-1-sulfonic acid Chemical compound COC1=CC(C)=CC(NCCCS(O)(=O)=O)=C1 DYPGTLUYQZIULN-UHFFFAOYSA-N 0.000 description 1
- BTIDJAQNJLWPTI-UHFFFAOYSA-N 3-(n-ethyl-3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonic acid Chemical compound OS(=O)(=O)CC(O)CN(CC)C1=CC(OC)=CC(OC)=C1 BTIDJAQNJLWPTI-UHFFFAOYSA-N 0.000 description 1
- CDGBQMHYFARRCC-UHFFFAOYSA-N 3-(n-ethyl-3,5-dimethylanilino)-2-hydroxypropane-1-sulfonic acid Chemical group OS(=O)(=O)CC(O)CN(CC)C1=CC(C)=CC(C)=C1 CDGBQMHYFARRCC-UHFFFAOYSA-N 0.000 description 1
- ZTQGWROHRVYSPW-UHFFFAOYSA-N 3-(n-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonic acid Chemical compound OS(=O)(=O)CC(O)CN(CC)C1=CC=CC(C)=C1 ZTQGWROHRVYSPW-UHFFFAOYSA-N 0.000 description 1
- IBSUMVZKDLDAEK-UHFFFAOYSA-N 3-(n-ethyl-3-methylanilino)propane-1-sulfonic acid Chemical compound OS(=O)(=O)CCCN(CC)C1=CC=CC(C)=C1 IBSUMVZKDLDAEK-UHFFFAOYSA-N 0.000 description 1
- NYLUYMWPXIIXDX-UHFFFAOYSA-N 3-(phenylazaniumyl)propane-1-sulfonate Chemical compound OS(=O)(=O)CCCNC1=CC=CC=C1 NYLUYMWPXIIXDX-UHFFFAOYSA-N 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 101100388509 Caenorhabditis elegans che-3 gene Proteins 0.000 description 1
- 102100032404 Cholinesterase Human genes 0.000 description 1
- 241001191009 Gymnomyza Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 101000943274 Homo sapiens Cholinesterase Proteins 0.000 description 1
- 108010013563 Lipoprotein Lipase Proteins 0.000 description 1
- 102100022119 Lipoprotein lipase Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 102100037097 Protein disulfide-isomerase A3 Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000011481 absorbance measurement Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- SRSXLGNVWSONIS-UHFFFAOYSA-N benzenesulfonic acid Chemical group OS(=O)(=O)C1=CC=CC=C1 SRSXLGNVWSONIS-UHFFFAOYSA-N 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 description 1
- NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 description 1
- 150000001840 cholesterol esters Chemical class 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
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- 238000010353 genetic engineering Methods 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
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- 238000001727 in vivo Methods 0.000 description 1
- 235000019626 lipase activity Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- DXYYSGDWQCSKKO-UHFFFAOYSA-N methylbenzothiazole Natural products C1=CC=C2SC(C)=NC2=C1 DXYYSGDWQCSKKO-UHFFFAOYSA-N 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- SVLRFMQGKVFRTB-UHFFFAOYSA-M sodium;3-(3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].COC1=CC(NCC(O)CS([O-])(=O)=O)=CC(OC)=C1 SVLRFMQGKVFRTB-UHFFFAOYSA-M 0.000 description 1
- HDARHUHTZKLJET-UHFFFAOYSA-M sodium;3-(n-ethyl-3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC(OC)=CC(OC)=C1 HDARHUHTZKLJET-UHFFFAOYSA-M 0.000 description 1
- HLXGRHNZZSMNRX-UHFFFAOYSA-M sodium;3-(n-ethyl-3,5-dimethylanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC(C)=CC(C)=C1 HLXGRHNZZSMNRX-UHFFFAOYSA-M 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/30—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving catalase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
- G01N2333/918—Carboxylic ester hydrolases (3.1.1)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Endocrinology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201510179746.8A CN104894220B (zh) | 2010-11-10 | 2011-11-09 | 残粒样脂蛋白胆甾醇的定量方法及用于其的试剂盒 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2010251511A JP5766426B2 (ja) | 2010-11-10 | 2010-11-10 | レムナント様リポ蛋白コレステロールの定量方法及びそのためのキット |
JP2010-251511 | 2010-11-10 | ||
PCT/JP2011/075837 WO2012063866A1 (ja) | 2010-11-10 | 2011-11-09 | レムナント様リポ蛋白コレステロールの定量方法及びそのためのキット |
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CN201510179746.8A Division CN104894220B (zh) | 2010-11-10 | 2011-11-09 | 残粒样脂蛋白胆甾醇的定量方法及用于其的试剂盒 |
Publications (2)
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CN103314113A CN103314113A (zh) | 2013-09-18 |
CN103314113B true CN103314113B (zh) | 2015-11-25 |
Family
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CN201510179746.8A Active CN104894220B (zh) | 2010-11-10 | 2011-11-09 | 残粒样脂蛋白胆甾醇的定量方法及用于其的试剂盒 |
CN201180054103.0A Active CN103314113B (zh) | 2010-11-10 | 2011-11-09 | 残粒样脂蛋白胆甾醇的定量方法及用于其的试剂盒 |
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Country Status (6)
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US (1) | US8906638B2 (zh) |
EP (1) | EP2639310B1 (zh) |
JP (1) | JP5766426B2 (zh) |
KR (1) | KR101871211B1 (zh) |
CN (2) | CN104894220B (zh) |
WO (1) | WO2012063866A1 (zh) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3225993B1 (en) * | 2015-05-29 | 2019-12-25 | Sysmex Corporation | Method for measuring cholesterol uptake capacity of lipoproteins |
JP6829009B2 (ja) * | 2016-05-24 | 2021-02-10 | デンカ株式会社 | トリグリセリドリッチリポ蛋白中のコレステロールの定量方法及び定量試薬 |
CN107561297B (zh) * | 2017-08-25 | 2019-12-10 | 宁波美康盛德医学检验所有限公司 | 用于血脂分型检测的试剂 |
JP7455674B2 (ja) | 2020-06-02 | 2024-03-26 | デンカ株式会社 | キットおよび方法 |
CN114214389B (zh) * | 2022-02-21 | 2022-07-12 | 北京沃森赛瑟生物技术有限公司 | 脂蛋白胆固醇检测方法和试剂盒 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101065495A (zh) * | 2004-11-29 | 2007-10-31 | 株式会社Jimro | 残粒样脂蛋白中胆固醇的测定方法 |
CN101326289A (zh) * | 2005-12-09 | 2008-12-17 | 协和梅迪克斯株式会社 | 残粒样脂蛋白中的胆固醇测定方法、试剂及试剂盒 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6479249B2 (en) | 1996-12-09 | 2002-11-12 | Denka Seiken Co., Ltd. | Method of determining cholesterol content of high-density lipoproteins |
ATE348339T1 (de) | 1997-04-14 | 2007-01-15 | Denka Seiken Kk | Methode zur quantifizierung von cholesterol in lipoproteinen geringer dichte |
JP4456715B2 (ja) | 2000-02-28 | 2010-04-28 | 協和メデックス株式会社 | レムナント様リポ蛋白中のコレステロールの測定方法および測定試薬 |
DE60318685T2 (de) * | 2002-01-30 | 2008-12-24 | Denka Seiken Co., Ltd. | Verfahren zur quantifizierung von cholesterin in hd-(high density)-lipoprotein und reagenszusammensetzungen |
US20090170139A1 (en) * | 2005-12-09 | 2009-07-02 | Kyowa Medex Co., Ltd. | Method, reagent and kit for measurement of cholesterol in remnant-like particles |
EP1975244A4 (en) | 2006-01-20 | 2009-06-17 | Jimro Co Ltd | PROCESS FOR CHOLESTERIN MEASUREMENT IN REMAINING LIPOPROTEINS |
JP5332611B2 (ja) * | 2007-01-15 | 2013-11-06 | 東洋紡株式会社 | 高密度リポ蛋白中コレステロールの測定方法およびその試薬 |
JP5728490B2 (ja) * | 2010-10-29 | 2015-06-03 | アークレイ株式会社 | 低密度リポタンパク質中のコレステロールの測定方法及び測定用キット |
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CN101326289A (zh) * | 2005-12-09 | 2008-12-17 | 协和梅迪克斯株式会社 | 残粒样脂蛋白中的胆固醇测定方法、试剂及试剂盒 |
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WO2012063866A1 (ja) | 2012-05-18 |
CN103314113A (zh) | 2013-09-18 |
KR101871211B1 (ko) | 2018-06-27 |
US8906638B2 (en) | 2014-12-09 |
KR20140027060A (ko) | 2014-03-06 |
EP2639310A4 (en) | 2014-04-02 |
JP5766426B2 (ja) | 2015-08-19 |
CN104894220A (zh) | 2015-09-09 |
EP2639310B1 (en) | 2017-10-25 |
EP2639310A1 (en) | 2013-09-18 |
CN104894220B (zh) | 2018-06-08 |
JP2012100581A (ja) | 2012-05-31 |
US20130230873A1 (en) | 2013-09-05 |
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Commission number: 4W110722 Conclusion of examination: Declare that the technical solution of "or cholesteryl esterase with subunits with molecular weight less than 40KD" in claim 1 submitted by the patentee of invention No. 201180054103.0 on September 18, 2020 and the part of the above technical solution of claim 1 cited in dependent claims 2-3 are invalid, The patent remains valid on the basis of the technical solution of "cholesteryl esterase with molecular weight less than 40kDa" in claim 1 and the part of the technical solution cited in dependent claims 2-3 Decision date of declaring invalidation: 20210224 Decision number of declaring invalidation: 48241 Denomination of invention: Kit for measuring cholesterol like protein and method thereof Granted publication date: 20151125 Patentee: DENKA Co.,Ltd. |
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