CN103251515B - Composition for whitening skin - Google Patents
Composition for whitening skin Download PDFInfo
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- CN103251515B CN103251515B CN201310156108.5A CN201310156108A CN103251515B CN 103251515 B CN103251515 B CN 103251515B CN 201310156108 A CN201310156108 A CN 201310156108A CN 103251515 B CN103251515 B CN 103251515B
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- arbutin
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- whitening product
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Abstract
The invention discloses a composition for whitening skin. The composition comprises phloretin, alpha-arbutin and resveratrol, wherein the ratio of the phloretin to the arbutin to the resveratrol is 2: 1: 1 to 5: 1: 1 in parts by weight; after the optimal proportioning range is determined, in prepared cosmetics, 0-0.5% of the above mixed complex is added at room temperature, and then stirred and bottled. Experimental results show that, on the premise of controlling 4% of total active substances, when the mass ratio of the phloretin to the arbutin to the resveratrol is 2: 1: 1, the monophenyl oxidase inhibiting capacity is the highest.
Description
Technical field
The present invention relates to cosmetics, relate in particular to a kind of skin lightening compositions.
Background technology
The safety of cosmetics and effect are the targets that new period cosmetics development and exploitation are pursued, natural biological extract conventionally have multiple action, effect lasting stability, action temperature and, widely applicable, have no side effect or advantage that side effect is very little, its research and development have been become to the recent tendency of complying with back to nature, science beauty treatment, science nursing idea.Natural biological extraction composition as active component in cosmetics mainly comes from plant, animal and some Marine microorganism at present, wherein deep to the research and development of plant extract, applies also extensive.
In recent years, domestic and international many scholars have carried out fruitful work to the exploitation of Chinese herbal medicine active component.At present, from Chinese Traditional Medicine and application experience among the people, arranging the natural active matter for cosmetics of excavating has hundreds of.
Phloretin is external research and development recently a kind of new type natural skin-whitening agents out, is mainly distributed in peel and the root bark of the rich fruits such as Fructus Mali pumilae, pears.Its moisture-keeping function is very strong, the water that the weight of energy absorption own is 4～5 times; Can promote absorbing of other functional factor in formula, make it give play to better effect; Anti-oxidation function is very strong, can remove the free radical in skin; Can stop carbohydrate content to enter epidermis cell, thereby suppress the excessive secretion of cutaneous gland, the vigorous type acne for the treatment of secretion; Can suppress melanocyte activity, various skin splashs are had to desalination effect etc.
Arbutin, as the important derivatives of hydroquinone, is very popular whitening agent.Arbutin is safe, and whitening effect is obvious, and its application is quite extensive with research.The source of arbutin comprises plant extract, plant tissue culture, enzyme process and organic synthesis.Wherein composite is because its purity is high, lighter color, and activity is also very high, and occupies an leading position on market.Arbutin whitening effect has obvious gap compared with kojic acid and derivant thereof.In difference research, show, arbutin tyrosinase inhibition rate is kojic acid 2～10 times.
Resveratrol is the class polyphenol compound being mainly present in the plants such as Rhizoma Polygoni Cuspidati, Semen Cassiae, Rhizoma et radix veratri (Radix Rhizoma Veratri), Fructus Vitis viniferae, having the biological actions such as antiinflammatory, anticancer, antioxidation, inhibition platelet aggregation, is the effective ingredient of plurality of Chinese treatment inflammation, lipid metabolism, heart disease etc.Because numerous disease is as relevant in DNA damage, lipid peroxidation that tumor, cardiovascular diseases, aging, radiation damage etc. all cause with free radical, therefore effectively antioxidant is significant in diseases prevention and treatment.
At present, the external development and utilization about the Natural antioxidant generally launches.And for China, the main fraud point existing is exactly that Natural antioxidant product category is single, productivity ratio is lower, and compound product is less.
Summary of the invention
The object of this invention is to provide a kind of skin lightening compositions.
Compositions provided by the invention, comprises phloretin, arbutin and resveratrol.
Described compositions also can only be comprised of said components.
Concrete, the mass ratio of described phloretin, arbutin and resveratrol is (2-5): 1:1.
More specifically, the mass ratio of described phloretin, arbutin and resveratrol is 2:1:1,3:1:1,4:1:1,5:1:1,2-4:1:1,2-3:1:1,3-5:1:1,3-4:1:1 or 4-5:1:1.
Described arbutin is specially alpha-arbutin.
In described compositions, also comprise water; Wherein, the quality percentage composition of phloretin in compositions be 0.2%, 0.24%, 0.25%, 0.27%, 0.29%, 0.2-0.29%, 0.2-0.27%, 0.2-0.24% or 0.24%-0.29%;
Arbutin and the resveratrol quality percentage composition in compositions is 0.055%, 0.065%, 0.08%, 0.1%, 0.125%, 0.055-0.1%, 0.065-0.1%, 0.08-0.1%, 0.055-0.125%, 0.065-0.125%, 0.08-0.125%, 0.055-0.08% or 0.65%-0.08%;
The method of the described compositions of preparation provided by the invention, comprises the steps: aforementioned each component to mix, and obtains described compositions.
The present invention also provides a kind of tyrosinase inhibitor or skin-whitening product, and its effective ingredient is aforementioned compositions provided by the invention.
The quality percentage composition of described compositions in described tyrosinase inhibitor or skin-whitening product is 0-0.5%, and is not 0.
Concrete, the quality percentage composition of described compositions in described tyrosinase inhibitor or skin-whitening product is 0.4% or 0.5% or 0.4%-0.5% or 0.2% or 0.3% or 0.2%-0.5% or 0.2%-0.4% or 0.2%-0.3% or 0.3%-0.5% or 0.3%-0.4% or 0.1% or 0.1%-0.5% or 0.1%-0.4%.
In addition, the application of the compositions that the invention described above provides in being prepared as follows product, also belongs to protection scope of the present invention:
1) product of restraint of tyrosinase;
2) there is the product of skin whitening efficacy.
The present invention is by tyrosinase inhibition rate testing experiment, proof is by after three kinds of pure natural plant extract phloretin, alpha-arbutin and resveratrol composite usage, evidence it can be than only using wherein a kind of more effectively activity of restraint of tyrosinase, and then can infer that this material is than simple use a kind of white-skinned face function that has more wherein.By irritating testing experiment, prove that phloretin provided by the invention, alpha-arbutin and resveratrol are composite to skin nonirritant, safe, can be used for the fields such as cosmetics.
specific embodiment mode
Below in conjunction with specific embodiment, the invention will be further described, and content described in literary composition if no special instructions, is quality percentage composition, described method if no special instructions, be conventional method, described raw material if no special instructions, all can be bought and obtain from open commercial sources.
Embodiment 1
According to mass ratio as follows, phloretin, alpha-arbutin and resveratrol are mixed, obtain sample 6,9,10 and 13;
And establish contrast, be also sample 1-5,7,8,11,12,14-16;
Sample 1 phloretin: alpha-arbutin: resveratrol is 1:0:0
Sample 2 phloretin: alpha-arbutin: resveratrol is 0:1:0
Sample 3 phloretin: alpha-arbutin: resveratrol is 0:0:1
Sample 4 phloretin: alpha-arbutin: resveratrol is 2:0:2
Sample 5 phloretin: alpha-arbutin: resveratrol is 2:2:0
Sample 6 phloretin: alpha-arbutin: resveratrol is 2:1:1
Sample 7 phloretin: alpha-arbutin: resveratrol is 3:2:0
Sample 8 phloretin: alpha-arbutin: resveratrol is 3:0:2
Sample 9 phloretin: alpha-arbutin: resveratrol is 3:1:1
Sample 10 phloretin: alpha-arbutin: resveratrol is 4:1:1
Sample 11 phloretin: alpha-arbutin: resveratrol is 4:2:0
Sample 12 phloretin: alpha-arbutin: resveratrol is 4:0:2
Sample 13 phloretin: alpha-arbutin: resveratrol is 5:1:1
Sample 14 phloretin: alpha-arbutin: resveratrol is 5:0:2
Sample 15 phloretin: alpha-arbutin: resveratrol is 5:2:0
The blank deionized water of sample 16
Above-mentioned sample is added in deionized water and standardize solution again, make the gross mass percentage composition of all components in sample be 0.4%, carry out tyrosinase inhibition rate test, every kind of sample test 3 times, calculates its meansigma methods.
Tyrosinase inhibition rate test be standard for testing the method for testing of whitening active substance whitening function.Its concrete grammar is as follows:
1. in the tyrosine solution of 0.3mg/ml, add the potassium phosphate solution of the 0.1M of 500 μ L, add the tester of 450 μ L, mix homogeneously in test tube, constant temperature is at 37 ℃;
2. add 50 μ L-Tyr enzymatic solution (concentration is 1540unit/ml), under 37 ℃ of environment, cultivate 10min;
3. with UV-VIS spectrophotometer, test the trap of 480nm.
According to following formula, calculate tyrosinase inhibition rate:
Suppression ratio=[(A-B)/A] * l00%
Wherein, the light absorption value that " A " is standard control, " B " is for being subject to the light absorption value of test solution (or positive control).
Acquired results is as shown in table 1.
The measurement result of table 1, tyrosinase inhibition rate
As shown in Table 1, control and add under the prerequisite that total amount is 0.4%, at phloretin, alpha-arbutin and resveratrol three compound, the inhibition of tryrosinase is all better than to single component or two kinds of composition compounds.
Mass ratio at phloretin, alpha-arbutin and resveratrol is that 2:1:1(is also sample 6) time, it is the strongest that its tryrosinase suppresses ability.
When ratio is greater than 2:1:1, its tryrosinase inhibition ability reduces.
Embodiment 2
By phloretin, alpha-arbutin and resveratrol complex composition, according to the highest ratio of tyrosinase inhibition rate, be phloretin: alpha-arbutin: the ratio that the mass ratio of resveratrol is 2:1:1 is added in deionized water and standardize solution, make the gross mass percentage composition of phloretin in mixed liquor, alpha-arbutin and resveratrol as follows, obtain sample 15-20;
Control sample 21 is set simultaneously;
Sample 16 0.1%
Sample 17 0.2%
Sample 18 0.3%
Sample 19 0.4%
Sample 20 0.5%
Sample 21 deionized waters
Utilize the method that embodiment 1 is identical to carry out tyrosinase inhibition rate test, acquired results is as shown in table 2.
Table 2, different percentage composition compositions tyrosinase inhibition rate measurement result
As shown in Table 2, mass ratio at phloretin, alpha-arbutin and resveratrol is 2:1:1, it adds total amount is 0.4%(counter sample 19) and 0.5%(counter sample 20) time, its inhibition ability to tryrosinase is the strongest, when 0.4% addition, suppression ratio reaches the highest, therefore the optimum of addition is 0.4%.
While using in cosmetics due to phloretin, alpha-arbutin and resveratrol, be all at room temperature to add, bottling after stirring; In the present embodiment, phloretin, alpha-arbutin and resveratrol are added to the tyrosinase inhibition rate testing experiment that deionized water carries out, acquired results is consistent with the result of the test again that in actual production, itself and other cosmetic composition stirred, thereby by the tyrosinase inhibition rate testing experiment of above-described embodiment 1 and 2, proof is by after three kinds of pure natural plant extract phloretin, alpha-arbutin and resveratrol composite usage, than only using wherein one or both more effectively activity of restraint of tyrosinase, have more white-skinned face function.
Safety testing: with reference to OECD Testing Guideline 439, utilize human body 3D skin model, the acute skin irritation of sample is tested.
Material: MTT and SDS, be all purchased from sigma company; The flat culture plate in Falcon24 hole;
Skin model: the attached culture medium of LabCyte EPI-MODEL24(), be purchased from Japanese J-TEC company;
Tested material:
Sample 1: phloretin
Sample 2: alpha-arbutin
Sample 3: resveratrol
Sample 4: phloretin: alpha-arbutin: compound resveratrol=2:1:1(mass ratio)
Sample 5: phloretin: alpha-arbutin: compound resveratrol=3:1:1(mass ratio)
Sample 6: phloretin: alpha-arbutin: compound resveratrol=4:1:1(mass ratio)
Sample 7: phloretin: alpha-arbutin: compound resveratrol=5:1:1(mass ratio)
Sample 8: distilled water (negative control)
Sample 9:5%SDS(positive control)
Method:
First 25 μ l distilled water are added to skin model surface, then each sample of 25mg is added to skin model surface, directly 25 μ l negative control distilled water and positive control 5%SDS are added to skin model surface, all act on 15min, parallel repetition 3 times.With PBS buffer, rinse tested material and stop exposing, then skin model is gone to fresh culture and hatch 42 hours at 37 ° of C.Hatch after end, skin model is placed in to MTT culture medium to be cultivated 3 hours, then epidermis is taken out and add isopropyl alcohol fully to extract in formazan formazan(refrigerator lucifuge over 15 hours), centrifugal, get supernatant and at 570nm and 650nm, survey OD value respectively, take isopropyl alcohol as blank, calculate the OD value recording, according to OD value, calculate relative activity (%).
Result treatment and calculating:
The OD value recording=(570nmOD sample-570nmOD is blank)-(650nmOD sample-650nmOD is blank)
Average OD value * 100 that average OD value/negative control that relative activity (%)=sample records records.
Result is judged:
Relative activity≤50%, tested material is irritant;
Relative activity >50%, tested material nonirritant
Acceptance standard: average OD value >=0.7 that negative control records: the test result of positive control average relative activity≤40% average OD value is in Table 3.
Test result after table 3, tested material and skin model effect 15min
As shown in Table 3, the equal >50% of cytoactive of phloretin, alpha-arbutin, resveratrol compound (counter sample 4-7), thinks to skin nonirritant according to criterion.
By irritating testing experiment, prove that the compound of phloretin provided by the invention, alpha-arbutin and resveratrol is to skin nonirritant, safe, can be used for the fields such as cosmetics.
Claims (20)
1. a compositions, comprises phloretin, arbutin and resveratrol; The mass ratio of described phloretin, arbutin and resveratrol is 2:1:1.
2. compositions according to claim 1, is characterized in that: described compositions is comprised of phloretin, arbutin and resveratrol.
3. compositions according to claim 1 and 2, is characterized in that: described arbutin is alpha-arbutin.
4. a method of preparing the arbitrary described compositions of claim 1-3, comprises the steps: arbitrary described each component of claim 1-3 to mix, and obtains described compositions.
5. tyrosinase inhibitor or a skin-whitening product, its effective ingredient is the arbitrary described compositions of claim 1-3.
6. inhibitor according to claim 5 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0-0.5%, and be not 0.
7. inhibitor according to claim 6 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.1%-0.5%.
8. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.1%-0.4%.
9. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.2%-0.5%.
10. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.2%-0.4%.
11. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is or 0.2%-0.3%.
12. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.3%-0.5%.
13. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.3%-0.4%.
14. inhibitor according to claim 5 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.4%-0.5%.
15. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.5%.
16. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.4%.
17. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.3%.
18. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.2%.
19. inhibitor according to claim 7 or skin-whitening product, is characterized in that: the quality percentage composition of described compositions in described inhibitor or skin-whitening product is 0.1%.
The application of the arbitrary described compositions of 20. claim 1-3 in being prepared as follows product:
1) product of restraint of tyrosinase;
2) there is the product of skin whitening efficacy.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310156108.5A CN103251515B (en) | 2013-04-28 | 2013-04-28 | Composition for whitening skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310156108.5A CN103251515B (en) | 2013-04-28 | 2013-04-28 | Composition for whitening skin |
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| Publication Number | Publication Date |
|---|---|
| CN103251515A CN103251515A (en) | 2013-08-21 |
| CN103251515B true CN103251515B (en) | 2014-12-03 |
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| CN201310156108.5A Active CN103251515B (en) | 2013-04-28 | 2013-04-28 | Composition for whitening skin |
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| CN (1) | CN103251515B (en) |
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2013
- 2013-04-28 CN CN201310156108.5A patent/CN103251515B/en active Active
Non-Patent Citations (4)
| Title |
|---|
| 《Resveratrol:an original mechanism on tyrosinase inhibition》;P.BERNARD et.al.;《International Journal of Cosmetic Science》;20001231;219－226 * |
| 《根皮素抑酪氨酸酶活性研究》;王建新等;《香料香精化妆品》;20020430(第2期);4-5 * |
| P.BERNARD et.al..《Resveratrol:an original mechanism on tyrosinase inhibition》.《International Journal of Cosmetic Science》.2000,219－226. * |
| 王建新等.《根皮素抑酪氨酸酶活性研究》.《香料香精化妆品》.2002,(第2期),4-5. * |
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| CN103251515A (en) | 2013-08-21 |
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