CN103238456A - Method for utilizing fritillaria cirrhosa flakes to quickly breed fritillaria cirrhosa - Google Patents
Method for utilizing fritillaria cirrhosa flakes to quickly breed fritillaria cirrhosa Download PDFInfo
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- CN103238456A CN103238456A CN2013102117354A CN201310211735A CN103238456A CN 103238456 A CN103238456 A CN 103238456A CN 2013102117354 A CN2013102117354 A CN 2013102117354A CN 201310211735 A CN201310211735 A CN 201310211735A CN 103238456 A CN103238456 A CN 103238456A
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Abstract
The invention discloses a method for quickly breeding fritillaria cirrhosa bulbs. The method mainly comprises the steps that fritillaria cirrhosa flakes are utilized for breeding, and fritillaria cirrhosa plants are cultivated quickly through dormancy break treatment, disinfection treatment, flake differentiation treatment and the like. The flakes are utilized for the breeding; the dormancy break treatment is conducted by hormones in a breeding process; the cultivation time is shortened; a tissue culture method is not adopted; in this case, requirements on a technology and an environment are reduced greatly; the cost is lowered; in the breeding process, each small flake can differentiate 4-12 small bulbs; in this case, use ratios of the two-stage and three-stage fritillaria cirrhosa are increased greatly; and the breeding efficiency of the fritillaria cirrhosa is improved.
Description
Technical field
The present invention relates to the propagation method of a kind of bulb of fritillary, be specifically related to a kind of method of utilizing scale to breed Bulbus Fritillariae Cirrhosae fast.
Background technology
The bulb of fritillary is herbaceos perennial, and its bulb hyoscine has effect relieving cough and reducing sputum, the heat-clearing dissipating bind; The bulb of fritillary " family " is different by the place of production and kind, can be divided into Bulbus Fritillariae Cirrhosae, fritillaria thunbergii and the rhizoma bolbostemmae, Siberian fritillary bulb four big classes.Wherein Bulbus Fritillariae Cirrhosae is the underground bulb of the bulbs of fritillary such as the Liliaceae herbaceos perennial crow flower bulb of fritillary, the leaf roll bulb of fritillary, the Luo Shi bulb of fritillary, the Gansu bulb of fritillary, the rib sand bulb of fritillary.Because main product is gained the name in Sichuan, but in Tibet, Gansu, Xinjiang, North China, northeast all produces.Bulbus Fritillariae Cirrhosae cold nature and sweet-bitter flavor, go into the cardiopulmonary warp, function moistening lung, cough-relieving, reduce phlegm, clinical normal and compatibilities such as the root of straight ladybell, the tuber of dwarf lilyturf, asparagus fern, mulberry leaf, chrysanthemum for hot phlegm, sticky sputum, deficiency syndrome of the lung labor cough, cough for a long time, phlegm is swallowed dry, blood-stained sputum less and treatment of conditions such as ambition pent-up, consumptive lung disease, lung carbuncle.Modern pharmacological research confirms that Bulbus Fritillariae Cirrhosae contains multiple alkaloids such as fritimine, and fritimine brings high blood pressure down, and multiple pharmacological effect such as excited uterus have high medical value.
The Bulbus Fritillariae Cirrhosae happiness weather conditions that cool, have cold-resistant, happiness is wet, be afraid of high humidity, characteristic that happiness covers.When temperature reached 30 ℃ or ground temperature and surpasses 25 ℃, plant will wither; Can not survive in the area that height above sea level is low, temperature is high.Do not plant having fully under the concealment condition, seedling easily dries up in flakes; Cross sunshine and can impel plant water evaporates and respiration to strengthen by force, easily cause the bulb index of aridity low, so the planting conditions of Bulbus Fritillariae Cirrhosae is relatively harsher, cause its price comparison height.Bulbus Fritillariae Cirrhosae has bulb and two kinds of propagation methods of seed.Because the reproduction coefficient of seminal propagation production cycle long (can gather in the crops in after planting 6 years) and fritillary bulb bulb very big (1 female bulb can grow the nearly daughter bulb of 20-50 different sizes in 1 year) is gone up the general bulb breeding method that adopts so produce.People are the fourth class with the bulb of gathering in the crops out with the hole sizer letter sorting at present: first-class processing is used as medicine; Planting of second-class (as the fibert size) can be gathered in the crops in back 1 year; 2 years results of need growth of third-class (as the corn size); The fourth class (third-class following person, minimum as the big grain of rice) needs results after 3 years.So little bulb especially time of needing of the bulb breeding of three fourth class long, in a disguised form increased production cost, to carry out reproductive efficiency low so directly plant method for planting according to routine, the Bulbus Fritillariae Cirrhosae bulb resource of waste preciousness.At present the tissue culture propagating technology to the bulb of fritillary also has a lot of research, and tissue is cultivated has higher technology content, to the requirement of plant also than higher.
Summary of the invention
The technical problem that the present invention mainly solves is to utilize Bulbus Fritillariae Cirrhosae scale fast breeding Bulbus Fritillariae Cirrhosae, has both overcome to utilize tissue to cultivate a difficult problem higher to specification requirement, can utilize Limited resources breeding Bulbus Fritillariae Cirrhosae plant again, and improves the reproduction speed of Bulbus Fritillariae Cirrhosae greatly.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
A kind of Bulbus Fritillariae Cirrhosae bulb is the method for breeding fast, it is characterized in that, may further comprise the steps:
(1) at first select for use through behind the sieving and grading as the second-class bulb of fibert size, place 45 ℃ of warm water to soak 90 minutes on bulb, pull the back out in the cold water cooling, and then irradiation was disinfected under the ultraviolet ray of 2500-2650A wave-length coverage in 10-20 minute; And then soak 20-30 minute with breaking dormancy at 500 mg/L GA.
(2) the Bulbus Fritillariae Cirrhosae bulb strip off in layer that will disinfect, in the process of strip off, to note observing the size of scale, when the diameter of scale during less than 10mm, just stop to continue to peel, select diameter to continue in 45 ℃ of warm water, to soak 30-40 minute greater than the ramentum of 10mm, pull the back out in the cold water cooling, and then irradiation 5-10min disinfects under the ultraviolet ray of 2500-2650A wave-length coverage; With matrix through the sterilization after, scale is layered on the matrix, and then covers the matrix of 3-4cm on the scale top of completing, put into 20-25 ℃ environment vernalization 40-50 days then, will control intensity of illumination at 1200-1400lex in the process of vernalization, the moisture of matrix is at 40-50%; In this process, go up gradually at scale and differentiate clove gradually; After the clove that differentiates reaches more than 90%, control temperature then between 16-20 ℃, intensity of illumination is controlled at 1500-1700lex, and to keep water supply in media content be 30-40%, continues to cultivate 40-70 days;
(3) clove that breaks up out through above-mentioned process at first utilizes the NAA of 300mg/L GA and 250 mg/L to handle to remove the dormancy of bulb; Then the Bulbus Fritillariae Cirrhosae bulb is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 50% ties plastic sack then, and the dislocation refrigerator-freezer is handled 25 d down for 0 ℃ and abolished dormancy again;
(4) will be through abolishing Bulbus Fritillariae Cirrhosae clove that dormancy handles 20 min that in potassium permanganate 0.1 % soup, sterilize, each clove that differentiation is come out strips down from stem then, continue to impel clove to take root with 500mg/L GA processing, each clove that differentiation is come out is transplanted breeding then; Cultural method is carried out according to conventional method.
When selecting propagating materials, also can select three grades of bulbs; Three grades of bulbs will select diameter greater than the scale of 10mm in the strip off process.Though three grades of scales can not all utilize, but in the process of strip off when finding that ramentum is less than 10mm, just stop to continue to peel, remaining clove can be sowed breed according to the standard of level Four bulb, so also improved the availability of three grades of scales.
The invention has the beneficial effects as follows:
(1) utilizes scale to breed, in reproductive process, utilize hormone to break dormancy and handle, shortened the cultivation time, and not by organizing cultured method, like this technology and environment requirement have been reduced greatly, also saved cost.
(2) in utilizing the scale reproductive process, utilize GA to urge root and handle, so also saved the breeding time.
(3) in breeding, each ramentum can differentiate 4-12 clove, and the availability of secondary and three grades of Bulbus Fritillariae Cirrhosaes is improved greatly like this, and has improved its reproductive efficiency.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that protection scope of the present invention is made more explicit defining.
Embodiment 1
(1) at first select for use through behind the sieving and grading as the second-class bulb of fibert size as propagating materials, place 45 ℃ of warm water to soak 90 minutes on bulb, pull the back out in the cold water cooling, and then irradiation was disinfected under the ultraviolet ray of 2500A wavelength in 20 minutes; And then soak 20 minutes with breaking dormancy at 500 mg/L GA;
(2) the Bulbus Fritillariae Cirrhosae bulb strip off in layer that will disinfect, the size that will note observing scale in the process of strip off when the diameter of scale during less than 10mm, just stops to continue to peel; Select diameter to continue in 45 ℃ of warm water, to soak 40 minutes greater than the ramentum of 10mm, pull the back out in the cold water cooling, and then irradiation 10min disinfects under the ultraviolet ray of 2500A wave-length coverage; Matrix after sterilization, is layered on scale on the matrix, and then covers the matrix of 3cm on the scale top of completing, put into 20 ℃ environment vernalization 50 days then, will control intensity of illumination at 1200lex in the process of vernalization, the moisture of matrix is 40%; In this process, go up gradually at scale and differentiate clove gradually; After the clove that differentiates reaches more than 90%, control temperature then at 16 ℃, intensity of illumination is controlled at 1500lex, and to keep water supply in media content be 30%, continues to cultivate 70 days;
(3) clove that breaks up out through above-mentioned process at first utilizes the NAA of 300mg/L GA and 250 mg/L to handle to remove the dormancy of bulb; Then the Bulbus Fritillariae Cirrhosae bulb is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 50% ties plastic sack then, and the dislocation refrigerator-freezer is handled 25 d down for 0 ℃ and abolished dormancy again;
(4) will be through abolishing Bulbus Fritillariae Cirrhosae clove that dormancy handles 20 min that in potassium permanganate 0.1 % soup, sterilize, each clove that differentiation is come out strips down from stem then, continue to impel clove to take root with 500mg/L GA processing, each clove that differentiation is come out is transplanted breeding then; Cultural method is carried out according to conventional method.
Embodiment 2
1, the method for the quick breeding of a kind of Bulbus Fritillariae Cirrhosae bulb is characterized in that, may further comprise the steps:
(1) at first select for use through behind the sieving and grading as the second-class bulb of fibert size as propagating materials, place 45 ℃ of warm water to soak 90 minutes on bulb, pull the back out in the cold water cooling, and then irradiation was disinfected under the ultraviolet ray of 2650A wave-length coverage in 10 minutes; And then soak 30 minutes with breaking dormancy at 500 mg/L GA;
(2) the Bulbus Fritillariae Cirrhosae bulb strip off in layer that will disinfect, the size that will note observing scale in the process of strip off when the diameter of scale during less than 10mm, just stops to continue to peel; Select diameter greater than the ramentum of 10mm, continue in 45 ℃ of warm water, to soak 40 minutes, pull the back out in the cold water cooling, and then irradiation 5min disinfects under the ultraviolet ray of 2650A wave-length coverage; Matrix after sterilization, is layered on scale on the matrix, and then covers the matrix of 4cm on the scale top of completing, put into 25 ℃ environment vernalization 40 days then, will control intensity of illumination at 1400lex in the process of vernalization, the moisture of matrix is 50%; In this process, go up gradually at scale and differentiate clove gradually; After the clove that differentiates reaches more than 90%, control temperature then at 20 ℃, intensity of illumination is controlled at 1700lex, and to keep water supply in media content be 40%, continues to cultivate 40 days;
(3) clove that breaks up out through above-mentioned process at first utilizes the NAA of 300mg/L GA and 250 mg/L to handle to remove the dormancy of bulb; Then the Bulbus Fritillariae Cirrhosae bulb is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 50% ties plastic sack then, and the dislocation refrigerator-freezer is handled 25 d down for 0 ℃ and abolished dormancy again;
(4) will be through abolishing Bulbus Fritillariae Cirrhosae clove that dormancy handles 20 min that in potassium permanganate 0.1 % soup, sterilize, each clove that differentiation is come out strips down from stem then, continue to impel clove to take root with 500mg/L GA processing, each clove that differentiation is come out is transplanted breeding then; Cultural method is carried out according to conventional method.
Embodiment 3
(1) at first select for use through behind the sieving and grading as the third-class bulb of fibert size as propagating materials, place 45 ℃ of warm water to soak 90 minutes on bulb, pull the back out in the cold water cooling, and then irradiation was disinfected under the ultraviolet ray of 2500A wave-length coverage in 10 minutes; And then soak 30 minutes with breaking dormancy at 500 mg/L GA;
(2) the Bulbus Fritillariae Cirrhosae bulb strip off in layer that will disinfect, the size that will note observing scale in the process of strip off when the diameter of scale during less than 10mm, just stops to continue to peel; Select diameter greater than the ramentum of 10mm, continue in 45 ℃ of warm water, to soak 30 minutes, pull the back out in the cold water cooling, and then irradiation 10min disinfects under the ultraviolet ray of 2500A wave-length coverage; Matrix after sterilization, is layered on scale on the matrix, and then covers the matrix of 3cm on the scale top of completing, put into 25 ℃ environment vernalization 50 days then, will control intensity of illumination at 1400lex in the process of vernalization, the moisture of matrix is 40%; In this process, go up gradually at scale and differentiate clove gradually; After the clove that differentiates reaches more than 90%, control temperature then between 16 ℃, intensity of illumination is controlled at 1700lex, and to keep water supply in media content be 40%, continues to cultivate 60 days;
(3) clove that breaks up out through above-mentioned process at first utilizes the NAA of 300mg/L GA and 250 mg/L to handle to remove the dormancy of bulb; Then the Bulbus Fritillariae Cirrhosae bulb is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 50% ties plastic sack then, and the dislocation refrigerator-freezer is handled 25 d down for 0 ℃ and abolished dormancy again;
(4) will be through abolishing Bulbus Fritillariae Cirrhosae clove that dormancy handles 20 min that in potassium permanganate 0.1 % soup, sterilize, each clove that differentiation is come out strips down from stem then, continue to impel clove to take root with 500mg/L GA processing, each clove that differentiation is come out is transplanted breeding then; Cultural method is carried out according to conventional method.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the present invention.
Claims (2)
1. the method that the Bulbus Fritillariae Cirrhosae bulb is bred fast is characterized in that, may further comprise the steps:
(1) at first select for use through behind the sieving and grading as the second-class bulb of fibert size as propagating materials, place 45 ℃ of warm water to soak 90 minutes on bulb, pull the back out in the cold water cooling, and then irradiation was disinfected under the ultraviolet ray of 2500-2650A wave-length coverage in 10-20 minute; And then soak 20-30 minute with breaking dormancy at 500 mg/L GA;
(2) the Bulbus Fritillariae Cirrhosae bulb strip off in layer that will disinfect, the size that will note observing scale in the process of strip off when the diameter of scale during less than 10mm, just stops to continue to peel; Select diameter greater than the ramentum of 10mm, continue in 45 ℃ of warm water, to soak 30-40 minute, pull the back out in the cold water cooling, and then irradiation 5-10min disinfects under the ultraviolet ray of 2500-2650A wave-length coverage; With matrix through the sterilization after, scale is layered on the matrix, and then covers the matrix of 3-4cm on the scale top of completing, put into 20-25 ℃ environment vernalization 40-50 days then, will control intensity of illumination at 1200-1400lex in the process of vernalization, the moisture of matrix is at 40-50%; In this process, go up gradually at scale and differentiate clove gradually; After the clove that differentiates reaches more than 90%, control temperature then between 16-20 ℃, intensity of illumination is controlled at 1500-1700lex, and to keep water supply in media content be 30-40%, continues to cultivate 40-70 days;
(3) clove that breaks up out through above-mentioned process at first utilizes the NAA of 300mg/L GA and 250 mg/L to handle to remove the dormancy of bulb; Then the Bulbus Fritillariae Cirrhosae bulb is mixed being placed in the plastic sack with silt, fill with the liver moss after the sterilization around the bulb, humidity about 50% ties plastic sack then, and the dislocation refrigerator-freezer is handled 25 d down for 0 ℃ and abolished dormancy again;
(4) will be through abolishing Bulbus Fritillariae Cirrhosae clove that dormancy handles 20 min that in potassium permanganate 0.1 % soup, sterilize, each clove that differentiation is come out strips down from stem then, continue to impel clove to take root with 500mg/L GA processing, each clove that differentiation is come out is transplanted breeding then; Cultural method is carried out according to conventional method.
2. the quick method of breeding of a kind of Bulbus Fritillariae Cirrhosae bulb according to claim 1, its propagating materials also can be three grades of Bulbus Fritillariae Cirrhosae bulbs, three grades of bulbs will select diameter greater than the scale of 10mm in the strip off process.
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Cited By (10)
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| CN103430744A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for rapid breeding of fritillaria cirrhosa by means of fritillaria cirrhosa scales |
| CN103493733A (en) * | 2013-09-24 | 2014-01-08 | 薛刚 | Method for quickly inducing generation of fritillaria cirrhosa bulb |
| CN104145677A (en) * | 2014-08-15 | 2014-11-19 | 甘肃农业大学 | Gansu fritillaria bulb clove breeding method |
| CN106489462A (en) * | 2015-09-07 | 2017-03-15 | 宁远县富鑫生态农业开发有限公司 | A kind of Lilium longiflorum rapid breeding method |
| CN106900769A (en) * | 2017-03-02 | 2017-06-30 | 申翰昭 | The growth regulating composition and its application process of bulbus fritillariae cirrhosae |
| CN107409721A (en) * | 2017-08-18 | 2017-12-01 | 吉林省农业科学院 | A kind of clonal cuttage and seedling culture method of clover |
| CN109169272A (en) * | 2018-09-03 | 2019-01-11 | 云南中医学院 | A kind of artificial fast numerous method of the rhizoma bolbostemmae |
| CN110856463A (en) * | 2018-08-24 | 2020-03-03 | 安徽安盛农业科技发展有限公司 | Tissue culture and rapid propagation method of red raspberries |
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
| CN115336530A (en) * | 2022-06-08 | 2022-11-15 | 后永强 | Gansu fritillaria bulb scale re-segmentation rapid propagation method |
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| CN103430744A (en) * | 2013-08-16 | 2013-12-11 | 苏州仁成生物科技有限公司 | Method for rapid breeding of fritillaria cirrhosa by means of fritillaria cirrhosa scales |
| CN103493733A (en) * | 2013-09-24 | 2014-01-08 | 薛刚 | Method for quickly inducing generation of fritillaria cirrhosa bulb |
| CN104145677A (en) * | 2014-08-15 | 2014-11-19 | 甘肃农业大学 | Gansu fritillaria bulb clove breeding method |
| CN104145677B (en) * | 2014-08-15 | 2016-03-02 | 甘肃农业大学 | A kind of Gansu bulb of fritillary bulb lobe propagation method |
| CN106489462A (en) * | 2015-09-07 | 2017-03-15 | 宁远县富鑫生态农业开发有限公司 | A kind of Lilium longiflorum rapid breeding method |
| CN106900769A (en) * | 2017-03-02 | 2017-06-30 | 申翰昭 | The growth regulating composition and its application process of bulbus fritillariae cirrhosae |
| CN107409721A (en) * | 2017-08-18 | 2017-12-01 | 吉林省农业科学院 | A kind of clonal cuttage and seedling culture method of clover |
| CN110856463A (en) * | 2018-08-24 | 2020-03-03 | 安徽安盛农业科技发展有限公司 | Tissue culture and rapid propagation method of red raspberries |
| CN109169272A (en) * | 2018-09-03 | 2019-01-11 | 云南中医学院 | A kind of artificial fast numerous method of the rhizoma bolbostemmae |
| CN111296211A (en) * | 2020-03-12 | 2020-06-19 | 重庆市药物种植研究所 | Rapid propagation method of fritillaria taipaiensis bulbs |
| CN115336530A (en) * | 2022-06-08 | 2022-11-15 | 后永强 | Gansu fritillaria bulb scale re-segmentation rapid propagation method |
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