CN103232942B - 绣线菊内生真菌的抗植物病原菌发酵液的制备方法 - Google Patents
绣线菊内生真菌的抗植物病原菌发酵液的制备方法 Download PDFInfo
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- CN103232942B CN103232942B CN201310138140.0A CN201310138140A CN103232942B CN 103232942 B CN103232942 B CN 103232942B CN 201310138140 A CN201310138140 A CN 201310138140A CN 103232942 B CN103232942 B CN 103232942B
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- 241000233866 Fungi Species 0.000 title claims abstract description 34
- 238000000855 fermentation Methods 0.000 title claims abstract description 20
- 230000004151 fermentation Effects 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 241001092387 Spiraea Species 0.000 title abstract description 9
- 241000233616 Phytophthora capsici Species 0.000 claims abstract description 12
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 11
- 238000011218 seed culture Methods 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 15
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 239000008188 pellet Substances 0.000 claims description 9
- 239000001965 potato dextrose agar Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 241001558165 Alternaria sp. Species 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- -1 20 grams Substances 0.000 claims description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 230000001775 anti-pathogenic effect Effects 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 229940066779 peptones Drugs 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000010452 phosphate Substances 0.000 claims description 3
- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 229940001516 sodium nitrate Drugs 0.000 claims description 3
- 235000010344 sodium nitrate Nutrition 0.000 claims description 3
- 239000004317 sodium nitrate Substances 0.000 claims description 3
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- 230000000694 effects Effects 0.000 description 4
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- 239000000401 methanolic extract Substances 0.000 description 4
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- 229920005989 resin Polymers 0.000 description 4
- 239000003905 agrochemical Substances 0.000 description 3
- 239000013543 active substance Substances 0.000 description 2
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- 229940088710 antibiotic agent Drugs 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
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- 230000002401 inhibitory effect Effects 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
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- 208000003251 Pruritus Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
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- 230000005764 inhibitory process Effects 0.000 description 1
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- 230000008635 plant growth Effects 0.000 description 1
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- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
样品 | 抑制率(%) |
菌丝甲醇提取物(200mg/l) | 22.18±1.55 |
菌丝甲醇提取物(500mg/l) | 36.47±0.42 |
发酵液树脂吸附后甲醇洗脱物(200mg/l) | 37.39±0.42 |
发酵液树脂吸附后甲醇洗脱物(500mg/l) | 62.86±1.08 |
Claims (1)
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104789613A (zh) * | 2014-11-13 | 2015-07-22 | 安徽农业大学 | 绣线菊内生真菌发酵液中抑菌活性组份的提取与分离方法 |
CN104782693A (zh) * | 2015-04-06 | 2015-07-22 | 孟凯涛 | 一种防治阳山水蜜桃褐霉菌的组合物的制备方法 |
CN106867542B (zh) * | 2015-12-14 | 2020-04-21 | 灵武市森保科技开发有限公司 | 一种设施蔬菜连作土壤调理剂及其制备方法 |
CN109280624B (zh) * | 2018-10-30 | 2021-05-28 | 宁夏医科大学 | 一株链格孢菌及其在拮抗马铃薯环腐病致病菌中的应用 |
CN113604363B (zh) * | 2021-08-18 | 2023-04-28 | 杨凌未来中科环保科技有限公司 | 一种茄链格孢菌及其应用 |
CN113773967B (zh) * | 2021-08-19 | 2023-04-28 | 杨凌未来中科环保科技有限公司 | 一种致密链格孢菌及其应用 |
Citations (4)
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---|---|---|---|---|
CN101676383A (zh) * | 2008-09-16 | 2010-03-24 | 黑龙江大学 | 可产生紫杉醇的链格孢属真菌 |
CN102204570A (zh) * | 2011-04-08 | 2011-10-05 | 中国计量学院 | 链格孢菌代谢产物在防治黄瓜立枯病菌中的应用 |
CN102204571A (zh) * | 2011-04-08 | 2011-10-05 | 中国计量学院 | 链格孢菌代谢产物在防治西瓜枯萎病菌中的应用 |
CN102217656A (zh) * | 2011-04-08 | 2011-10-19 | 中国计量学院 | 链格孢菌代谢产物在防治番茄灰霉病菌中的应用 |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101676383A (zh) * | 2008-09-16 | 2010-03-24 | 黑龙江大学 | 可产生紫杉醇的链格孢属真菌 |
CN102204570A (zh) * | 2011-04-08 | 2011-10-05 | 中国计量学院 | 链格孢菌代谢产物在防治黄瓜立枯病菌中的应用 |
CN102204571A (zh) * | 2011-04-08 | 2011-10-05 | 中国计量学院 | 链格孢菌代谢产物在防治西瓜枯萎病菌中的应用 |
CN102217656A (zh) * | 2011-04-08 | 2011-10-19 | 中国计量学院 | 链格孢菌代谢产物在防治番茄灰霉病菌中的应用 |
Non-Patent Citations (2)
Title |
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产紫杉烷类物质的链格孢1011菌种的培养和发酵条件;项勇等;《东北林业大学学报》;20020531;第30卷(第3期);第17-20页 * |
项勇等.产紫杉烷类物质的链格孢1011菌种的培养和发酵条件.《东北林业大学学报》.2002,第30卷(第3期), |
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Inventor after: Hua Rimao Inventor after: Bai Yu Inventor after: Xu Qingqing Inventor after: Zeng Xianyin Inventor after: Cao Haiqun Inventor after: Wu Xiangwei Inventor before: Hua Rimao Inventor before: Xu Qingqing Inventor before: Bai Yu Inventor before: Zeng Xianyin Inventor before: Cao Haiqun Inventor before: Wu Xiangwei |
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Free format text: CORRECT: INVENTOR; FROM: HUA RIMAO XU QINGQING BAI YU CENG XIANYIN CAO HAIQUN WU XIANGWEI TO: HUA RIMAO BAI YU XU QINGQING CENG XIANYIN CAO HAIQUN WU XIANGWEI |
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