CN103189509A - 核酸的检测方法、样品的光学观察方法及荧光物质 - Google Patents

核酸的检测方法、样品的光学观察方法及荧光物质 Download PDF

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Publication number
CN103189509A
CN103189509A CN2011800532046A CN201180053204A CN103189509A CN 103189509 A CN103189509 A CN 103189509A CN 2011800532046 A CN2011800532046 A CN 2011800532046A CN 201180053204 A CN201180053204 A CN 201180053204A CN 103189509 A CN103189509 A CN 103189509A
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China
Prior art keywords
fluorescence
copper
nucleic acid
sample
light
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CN2011800532046A
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English (en)
Chinese (zh)
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新田尚
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Sony Corp
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Sony Corp
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Publication of CN103189509A publication Critical patent/CN103189509A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN2011800532046A 2010-11-11 2011-11-04 核酸的检测方法、样品的光学观察方法及荧光物质 Pending CN103189509A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2010252517 2010-11-11
JP2010-252517 2010-11-11
JP2011-201542 2011-09-15
JP2011201542A JP6003033B2 (ja) 2010-11-11 2011-09-15 核酸の検出方法及びサンプルの光学観察方法並びに蛍光体
PCT/JP2011/006163 WO2012063441A1 (ja) 2010-11-11 2011-11-04 核酸の検出方法及びサンプルの光学観察方法並びに蛍光体

Publications (1)

Publication Number Publication Date
CN103189509A true CN103189509A (zh) 2013-07-03

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CN2011800532046A Pending CN103189509A (zh) 2010-11-11 2011-11-04 核酸的检测方法、样品的光学观察方法及荧光物质

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Country Link
US (2) US9459213B2 (enExample)
EP (2) EP2639304B1 (enExample)
JP (1) JP6003033B2 (enExample)
CN (1) CN103189509A (enExample)
WO (1) WO2012063441A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6074911B2 (ja) 2012-05-10 2017-02-08 ソニー株式会社 核酸解析用マイクロチップ
JP6414238B2 (ja) * 2017-01-12 2018-10-31 ソニー株式会社 核酸解析用マイクロチップ
CA3107890A1 (en) * 2018-08-21 2020-02-27 Deep Genomics Incorporated Therapeutic splice-switching oligonucleotides
CN111323396A (zh) * 2018-12-14 2020-06-23 中国林业科学研究院资源昆虫研究所 一种测定紫胶色酸a含量的方法
CN112823213A (zh) * 2019-05-31 2021-05-18 福瑞诺姆控股公司 用于甲基化核酸的高深度测序的方法和系统
JP2021097648A (ja) * 2019-12-23 2021-07-01 横河電機株式会社 核酸配列計測装置及び核酸配列計測方法
CN113252620B (zh) * 2020-02-12 2025-09-05 青岛科技大学 一种可同时检测Hg2+和Ag+的方法

Citations (1)

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CN101168773A (zh) * 2007-10-16 2008-04-30 东南大学 一种基于荧光淬灭的核酸测序方法

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US4969924A (en) 1989-05-18 1990-11-13 General Motors Corporation Electro-optical connector plug
US5259050A (en) 1991-09-04 1993-11-02 The Furukawa Electric Co., Ltd. Multicore optical connector with partitions to separate the optical fibers into groups
US20040086917A1 (en) * 1995-09-27 2004-05-06 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
JP3975042B2 (ja) * 1999-12-27 2007-09-12 富士フイルム株式会社 固相担体表面へのｄｎａ断片の固定方法及びｄｎａチップ
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EP1470254A2 (en) * 2002-01-30 2004-10-27 Epigenomics AG Method for the analysis of cytosine methylation patterns
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VSEVOLOD V. ROSTOVTSEV ET AL.: "A Stepwise Huisgen Cycloaddition Process：Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes", 《ANGEW. CHEM. INT. ED.》, vol. 41, no. 14, 15 July 2002 (2002-07-15), pages 2596 - 2599 *
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Publication number Publication date
US9459213B2 (en) 2016-10-04
US20170081711A1 (en) 2017-03-23
US10081830B2 (en) 2018-09-25
JP6003033B2 (ja) 2016-10-05
JP2012118051A (ja) 2012-06-21
EP3269810A1 (en) 2018-01-17
EP2639304A1 (en) 2013-09-18
US20140038176A1 (en) 2014-02-06
EP2639304B1 (en) 2017-05-17
EP3269810B1 (en) 2021-03-17
WO2012063441A1 (ja) 2012-05-18
EP2639304A4 (en) 2014-03-26

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Application publication date: 20130703