CN103184140A - Photosynthetic microbial culture apparatus and mounting method thereof - Google Patents

Photosynthetic microbial culture apparatus and mounting method thereof Download PDF

Info

Publication number
CN103184140A
CN103184140A CN2011104466168A CN201110446616A CN103184140A CN 103184140 A CN103184140 A CN 103184140A CN 2011104466168 A CN2011104466168 A CN 2011104466168A CN 201110446616 A CN201110446616 A CN 201110446616A CN 103184140 A CN103184140 A CN 103184140A
Authority
CN
China
Prior art keywords
silk screen
culture apparatus
girder
curb girder
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2011104466168A
Other languages
Chinese (zh)
Other versions
CN103184140B (en
Inventor
朱振旗
王琳
陈彦平
刘世泉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ENN Science and Technology Development Co Ltd
Original Assignee
ENN Science and Technology Development Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ENN Science and Technology Development Co Ltd filed Critical ENN Science and Technology Development Co Ltd
Priority to CN201110446616.8A priority Critical patent/CN103184140B/en
Publication of CN103184140A publication Critical patent/CN103184140A/en
Application granted granted Critical
Publication of CN103184140B publication Critical patent/CN103184140B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/26Constructional details, e.g. recesses, hinges flexible
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a culture apparatus and a mounting method thereof. The culture apparatus includes a main body composed of at least two brackets. The main body is provided with a wire net for connecting the brackets. The bracket is of a triangle structure with at least one movable opening side and is composed of a bottom beam and at least a first side beam and a second side beam. The main body is inside provided with a transparent culture vessel fitted with the inner sides of the at least two brackets. The culture apparatus of the invention is low in center of gravity, good in structural stability, low in requirement for flatness of the ground, and suitable for outdoor large-scale farming. According to the present invention, the triangle structure capable of movably opening and closing is used. The wire net at one side can be opened to facilitate the assembling and unassembling of plastic bags. Further, the culture apparatus is simple in structure and good in airtight, and is suitable for outdoor industrial scale breeding. Moreover, the raw materials of the culture apparatus can be processed into preformed units in the factory, on-site installation workload is small, and operation is easy.

Description

A kind of photosynthetic microorganism culture apparatus and installation method thereof
Technical field
The present invention relates to a kind of bio-reactor, particularly relate to a kind of photosynthetic microorganism culture apparatus and installation method thereof.
Background technology
Photosynthetic microorganism or vegetable cell can utilize form inorganicss such as sun power, water and simple mineral substance synthesis of organic substance and hydrogen, methane.Little algae is exactly one of them typical case representative, and it is the individual small photosynthetic autotrophs unicellular organism of a class, has characteristics such as widely distributed, of a great variety, that photosynthetic efficiency is high, fast growth, adaptability are strong.The annual fixing CO of little algae 2Account for 40% of global net photosynthesis output, add it and be rich in high value natural pigments such as ester class, hydro carbons, albumen, soluble polysaccharide, astaxanthin, β-Hu Luobusu, therefore in today that problems such as environmental protection, the energy and health attract tremendous attention, little algae more and more is subjected to people's attention.
Extensive, the low-cost breed of little algae is the bottleneck of the little algae industrialized development of restriction, and exploitation simple and easy, the high-efficient culture device is the key of micro-algae large-scale cultivation.Pillar, tubular type, type such as board-like have successively appearred in the research and development of culture apparatus, in recent years because board-like photosynthetic microorganism culture apparatus in the advantage of aspects such as photosynthetic efficiency, has become the industry focal point.Board-like photosynthetic microorganism culture apparatus is gained the name because its outward appearance is tabular (rectangular-shaped), mainly comprises mechanically resistant material formula and soft material formula two big classes.The board-like photosynthetic microorganism culture apparatus of mechanically resistant material is made of transparent stiff board material such as sheet glass or PVC plates, also can be aided with skeleton construction sometimes; The board-like photosynthetic microorganism culture apparatus of soft material is made of wire frame and plastics bag, and is more simple with the board-like photosynthetic microorganism culture apparatus structure compared of mechanically resistant material, cost is more cheap, be easier to industrialization amplifies.But it still exists the structural stability deficiency, plastics bag is changed problems such as difficulty.
United States Patent (USP) WO2004/074423A2, WO2005/006838 and Chinese patent 200720023436.8 disclose the board-like photosynthetic microorganism culture apparatus that is made of silk screen and plastics bag.These board-like photosynthetic microorganism culture apparatuses are made of silk screen, skeleton construction and plastics bag.Silk screen and skeleton construction constitute tabular cavity, and intensity is provided, and keep the profile of culture apparatus; Plastics bag is lining in the silk screen inside cavity, and culture is housed.By pneumatic tube input air and carbonic acid gas in the culture apparatus, realize that carbon source is supplied with and the circulation stirring of nutrient solution, to carry out the breed of culture.
Mainly there is following technological deficiency in above-mentioned board-like photosynthetic microorganism culture apparatus: 1, stability of structure deficiency, because its radial section is the narrower rectangle in base, so center of gravity is higher, thereby the planeness on ground is had relatively high expectations; 2, Zhuan Zhi handiness is not high, makes the dismounting difficulty of plastics bag; Owing to for guaranteeing the culture quality, need the periodic replacement plastics bag in the breeding process of culture, to narrow and grow and spreads plastics bag in the silk screen cavity of (culture apparatus may reach tens to rice up to a hundred when mass-producing was used), actually operating is very difficult.
Summary of the invention
The objective of the invention is to propose a kind of simple in structure, be convenient to installing/dismounting, lower, the constitutionally stable trilateral photosynthetic microorganism of center of gravity culture apparatus, thereby increase operability and the environmental compatibility of culture apparatus.
For achieving the above object, the invention provides a kind of culture apparatus, described culture apparatus comprises the body that is made of at least two pack supports, also be provided with the silk screen that connects described each pack support on the described body, described support is the triangular structure of the movable switching at least on one side that is made of bottom girder and at least the first curb girder and second curb girder, and described body interior is provided with the transparent culture vessel that matches with described at least two pack support inboards.
Preferably, described first curb girder and described bottom girder be for fixedlying connected, and described second curb girder and described bottom girder be for being rotationally connected, between described second curb girder and described first curb girder for flexibly connecting.
More preferably, described transparent culture vessel is the tubular plastics bag, and described plastics bag top is provided with the hot pressing broadside, and described hot pressing broadside is provided with at least one suppending hole.
Further, described at least two pack supports are arranged in array.
Preferably, described plastics bag is provided with outer catheter and/or detects mouth.
More preferably, the bottom of described plastics bag is provided with parallel gas feed mechanism.
Preferably, described culture apparatus also comprises the mixing pit that links to each other with described transparent culture vessel by pipeline and pump.
Preferably, described culture apparatus comprises two groups or more body, is provided with a mixing pit between the two adjacent groups body at least.
Preferably, described silk screen comprises the first side silk screen that connects described at least two pack supports first curb girder, the second side silk screen that connects described at least two pack supports second curb girder, and the bottom surface silk screen that connects at least two pack support bottom girders.
More preferably, the bottom mesh-density of the described first side silk screen and the second side silk screen is greater than upper grid density.
Preferably, flexibly connect the outside of holding at described bottom girder and second curb girder, also be provided with locating part.
Another object of the present invention is to provide a kind of installation method of culture apparatus, this installation method comprises the steps: to make auxiliary mould, offers two grooves that match with curb girder and/or bottom girder width dimensions according to the distance of two adjacent groups support on mechanically resistant material; The assembling support, first curb girder and bottom girder installation combination is fixing, and will be rotationally connected between second curb girder and the bottom girder; The assembling body uses auxiliary mould, and first curb girder and bottom girder that at least two groups are fixed as one are formed array; After each bottom girder is laid the bottom surface silk screen, silk screen bottom, first side high spot is inserted in the slit at place, the long limit of bottom surface silk screen, and use auxiliary mould with working fastening between the first side silk screen and first curb girder; Transparent culture vessel is installed, transparent culture vessel is suspended on the prominence at silk screen top, first side; After inserting silk screen bottom, second side high spot in the slit of locating on silk screen long limit in bottom surface, second curb girder is arranged on the periphery of the second side silk screen, and buckles the active connection on first curb girder and the second curb girder top.
Preferably, described method also is included in the step that outer upper that described bottom girder and second curb girder flexibly connect end arranges locating part.
Based on technique scheme, advantage of the present invention is:
Culture apparatus center of gravity of the present invention is lower, and structural stability is better, and is less demanding to the planeness on ground, is more suitable for outdoor large-scale cultivation; The present invention has adopted the triangular structure of movable switching, and at least one side silk screen can be opened, and is convenient to loading and unloading and the replacing of plastics bag; In addition, the present invention is simple in structure, stopping property is better, is a kind of cheap closed culture apparatus that outdoor industrially scalable is cultured that is suitable for; And starting material of the present invention can become prefabricated component in factory process in advance, and the on-site installation work amount is less, and is easy and simple to handle.
Description of drawings
Accompanying drawing described herein is used to provide further understanding of the present invention, constitutes the application's a part, and illustrative examples of the present invention and explanation thereof are used for explaining the present invention, do not constitute improper restriction of the present invention.In the accompanying drawings:
Fig. 1 is the structural representation of a kind of preferred embodiment of culture apparatus body of the present invention;
Fig. 2 is the side-looking structural representation of a kind of embodiment of culture vessel of the present invention;
Fig. 3 is the elevation cross-sectional view of structure shown in Figure 2;
Fig. 4 is the synoptic diagram of the initial installment state of installation method of the present invention;
Fig. 5 is the enlarged diagram of installation method auxiliary mould of the present invention;
Fig. 6 is the synoptic diagram of installment state in the middle of the installation method of the present invention;
Fig. 7 is the structural representation of the another kind of preferred embodiment of culture apparatus body of the present invention;
Fig. 8 is the side-looking structural representation of the another kind of embodiment of culture vessel of the present invention;
Fig. 9 is the elevation cross-sectional view of structure shown in Figure 8;
Figure 10, Figure 11 are the structural representation of active connection of the present invention;
Figure 12 is the structural representation of another preferred embodiment of culture apparatus body of the present invention;
Figure 13 is the structural representation of another embodiment of culture apparatus of the present invention.
Embodiment
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Embodiment 1
As shown in Figure 1, culture apparatus of the present invention mainly is made up of body and transparent culture vessel two portions, and wherein body is formed jointly by at least two pack supports, and preferably, described at least two pack supports are arranged in array, and for example parallel being arranged in a row forms body part; Also be provided with the silk screen that connects described each pack support on the described body.Support is the triangular structure of the movable switching at least on one side that is made of bottom girder 5 and at least the first curb girder 1 and second curb girder 2.Preferably, first curb girder 1 and bottom girder 5 link to each other by fixed connecting piece 8 at the place, base angle or directly weldering extremely, second curb girder 2 is located at the base angle to link to each other by rotating connector 9 with bottom girder 5, namely second curb girder 2 is connected with bottom girder 5, but the part 9 that is dynamically connected of can rotating rotates.First curb girder 1 links to each other by active connection 7 at the drift angle place with second curb girder 2, and namely first curb girder 1 can be opened as required at any time with being connected of second curb girder 2.So, first curb girder 1, second curb girder 2 and bottom girder 5 have just constituted stable triangular supports structure.A plurality of such triangular supports are arranged in array, and have just formed body construction of the present invention.Bottom surface silk screen 6 is tiled in each bottom girder 5 place, and the first side silk screen 3 is positioned at first curb girder, 1 inboard, and the second side silk screen 4 is positioned at second curb girder, 2 inboards.
As shown in Figures 2 and 3, culture vessel of the present invention is made by transparent material, is preferably tubular plastics bag 11.In the present embodiment, plastics bag 11 is oval tubular, and the top of plastics bag 11 is provided with hot pressing broadside 10 along its length, so that operations such as installation, suspensions.On hot pressing broadside 10, be equipped with a suppending hole 14 at a certain distance along plastics bag 11 length directions, install and can plastics bag 11 integral body be hung over the prominence on the first side silk screen 3 or the second side silk screen, 4 tops by suppending hole 14 when changing plastics bag, so that subsequent operationss such as cultivation, loading and unloading.The broadside of hot pressing simultaneously 10 places also leave several and make the plastics bag 11 inner outer catheters 12 that communicate with external environment and/or detect mouth 13.In the present embodiment, both be provided with outer catheter 12 on the plastics bag 11, and also be provided with and detect mouth 13.Outer catheter 12 is used for the discharge of the interior gas of plastics bag and foam solution, detects mouth 13 and is used for measuring insertions of using instrument, probe and water supply, air-supply duct.Further preferably, water port 15 also is equipped with in the bottom of plastics bag 11, and nutrient solution can be discharged plastics bag 11 through water port 15 after culturing process was finished.
Further, the plastics bag 11 of present embodiment can directly (annotate: the plastic cloth that now sell in market all be to be long tubular with commercially available transparent plastic material, and roll up along its length) seal afterwards and directly use, and open several holes at it along its length near position of triangular supports drift angle, for liquid, the usefulness of the insertion of input, output and the detector probe of gas.The internal circumference of the triangular supports that the girth of plastics bag cross section surrounds smaller or equal to silk screen.And in the use, the nutrient solutions in the plastics bag 11 are not exclusively filled, and liquid level keeps certain distance apart from the plastics bag top, in case in the plastics bag during ventilation liquid from the hole at top, overflow.This kind technical scheme can greatly be dwindled manufacturing cost of the present invention, and reaches culturing purposes.
The technical scheme of present embodiment can solve effectively in the prior art that the culture apparatus center of gravity is too high, stability is not enough, and then the technical problem that ground flat degree is had relatively high expectations; Also can remedy prior art and mostly be fixedly connected, plastics bag loading and unloading difficulty, and occur in the culturing process be difficult to problem such as discharge as dirts such as foams.
Embodiment 1 described Unit Installation method comprises the steps:
As Fig. 4, Fig. 5 and shown in Figure 6, first curb girder 1, second curb girder 2, bottom girder 5 are made by wide 5 centimetres, thick 3 centimetres galvanized steel in the present embodiment.1.4 meters of first curb girder, 1 length, 48 centimetres of bottom girder 5 length, first curb girder 1 links to each other by fixed connecting piece 8 at the base angle with bottom girder 5, and preferably, in the present embodiment, first curb girder 1 is dead in the weldering of fixed connection place, base angle with bottom girder 5.Auxiliary mould 16 is had certain intensity, is convenient to cut or plastic mechanically resistant material is made by wooden, porous plastics or bakelite etc.Two grooves 17 of mould are stuck on two adjacent curb girders (as Fig. 4 and shown in Figure 6), and in the present embodiment, adjacent curb girder is tiled in bottom surface silk screen 6 on all bottom girders 5 at a distance of 0.5 meter again.Second curb girder 2 is connected by bolt with bottom girder 5 for long 1.4 meters, and bolt is also not exclusively tightened, and makes between bottom girder 5 and second curb girder 2 to have 1 millimeter space, and second curb girder 2 can be rotated round bolt.Certainly, also can adopt other active connections or mode of connection between bottom girder 5 and second curb girder 2.
The first side silk screen 3 and the second side silk screen 4 are high 1.3 meters, moulding silk screen by 5 millimeters thick linings constitutes, the bottom mesh-density of the described first side silk screen 3 and the second side silk screen 4 is greater than upper grid density, in the present embodiment, preferably silk screen is the grid of 5 centimeter square below the height for 0.5 meter, 0.5 be long 10 centimetres wide 5 centimetres rectangle grid more than the rice height, this kind structure makes wins side silk screen 3 and the second side silk screen 4 after plastics bag 11 fills with nutrient solution, can provide enough support strengths for body and plastics bag 11.
Further, the bottom high spot of the first side silk screen 3 is inserted in the narrow slit at place, bottom surface silk screen 6 long limits, again with mould with itself and 1 working fastening of first curb girder.Plastics bag 11 is made by 1 mm thick transparent plastic film, is oval tubular, and opening end seals through heat seal.The hot pressing broadside 10 that extrudes 4 cm widths through hot pressing is gone up at the top along its length.It is 1 centimetre suppending hole 14 that top flanging place is equipped with diameter along the plastics bag length direction every 0.5 meter.Top flanging place simultaneously also leaves 1 every 1.5 meters and makes the inner outer catheter 12 that communicates with external environment of plastics bag, outer catheter 12 constitutes for the tubule (0.6 centimetre of diameter) made by mechanically resistant material or by the extension (0.6 centimetre of diameter) of plastics, also leaves every 2 meters and detects mouthfuls 13.Plastics bag 11 bottoms are handled through the heat seal thermoplastic water port 15 and water discharge valve 22 are housed.The suppending hole 14 of plastics bag 11 is enclosed within the prominence at the first side silk screen, 3 tops, bottom high spot with the second side silk screen 4 inserts in the narrow slit at place, bottom surface silk screen 6 long limit again, at last second curb girder 2 is wrapped in the second side silk screen, 4 peripheries, and buckle the active connection 7 on first curb girder 1 and second curb girder, 2 tops.As shown in Figure 10 and Figure 11, the described active connection 7 of present embodiment comprises first part 29 and the second section 30 of the buckle that cooperatively interacts, this buckle locking structure, make simple, easy to operate, as can to make support body of the present invention constitute Stability Analysis of Structures, can open as required at any time by active connection 7 triangular structure.
At last, as shown in Figure 7, flexibly connect the top, the outside (for the inboard that culture vessel is housed) of holding at each bottom girder 5 and second curb girder 2, a locating part is set respectively.Locating part described in the present embodiment is the angle steel 19 of 60 degree for 10 centimetres of length, inclination angle, thereby can limit the scope of activity of second curb girder 2, as shown in Figure 7.
In addition, as Fig. 8 and shown in Figure 9, for reaching carbon source supply preferably and circulation mixing effect, in the bottom of plastics bag 11 parallel gas feed mechanism 20 is set also, this parallel gas feed mechanism 20 carries out air feed from the outside to plastics bag in 11 by airway 21, cultivates requirement thereby satisfy better.
Embodiment 2
Referring to Figure 12, in the present embodiment, 1.5 meters of first curb girder, 1 length, second curb girder, 2 1.8 meters of length, bottom girder 5 are grown 1 meter, three beam form right angle trilaterals, this kind body construction can satisfy the arrangement requirement near places such as board walls, and other structures are identical with above-described embodiment 1.
Embodiment 3
As shown in figure 13, in the plastics bag 11 gas supply mechanism is not housed in the present embodiment, and described culture apparatus comprises also and the described mixing pit 27 or 28 that links to each other with described transparent culture vessel by pipeline and pump 29, referring to Figure 13.Described culture apparatus comprises two groups or more body (shown in two bodies 26 among Figure 13) and transparent culture vessel, links to each other by mixing pit 27 or 28 between the transparent culture vessel of two adjacent groups.The shape of mixing pit both can be high and narrow, shown in the mixing pit 27 as shown in Figure 13; Also can be short and wide, shown in the mixing pit 28 as shown in Figure 13, its shape without limits, material printing opacity or light tightly all can.Carbonic acid gas is supplied with, nutritive salt replenishes, and reaches the results process and all can carry out in mixing pit, and the transparent culture vessel in the culture apparatus body is communicated with mixing pit, and nutrient solution flows into mixing pit voluntarily.In mixing pit, nutrient solution fully contacts with carbonic acid gas or nutrition, discharges the oxygen in the solution simultaneously, through impeller pump 29 nutrient solution in the mixing pit is injected another again and organizes intrinsic transparent culture vessel.At least being provided with a mixing pit between the transparent culture vessel of two adjacent groups links to each other respectively with both.
And, the present invention both can two groups of bodies (containing transparent culture vessel) as shown in figure 13, form the loop line; Also can link to each other with mixing pit by the mode of at least two group bodies (containing transparent culture vessel) with series, parallel and series-parallel connection, realize large-scale cultivation.
Need to prove that the making material of body of the present invention and transparent culture vessel is not limited to the material described in the embodiment, any material that can satisfy culture apparatus desirable strength, toughness and corrosion resistant requirement all can be realized the present invention.
In the breeding process of each embodiment; can in nutrient solution, add the solid particulate close with nutrient solution density; as plastic pellet; make it to coexist with nutrient solution one and flow in the reactor; solid particulate can produce collision with plastics bag in this process; have certain cleaning action, can reduce frustule to a certain extent in the absorption of plastic bag surface.
Obviously, those of ordinary skill in the art can constitute various types of biological culture instruments with a kind of culture apparatus of the present invention and installation method thereof.
Should be noted that at last: above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; Although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the field are to be understood that: still can make amendment or the part technical characterictic is equal to replacement the specific embodiment of the present invention; And not breaking away from the spirit of technical solution of the present invention, it all should be encompassed in the middle of the technical scheme scope that the present invention asks for protection.

Claims (13)

1. photosynthetic little algae biological culture device, it is characterized in that: described culture apparatus comprises the body that is made of at least two pack supports, also be provided with the silk screen that connects described each pack support on the described body, described support is that described body interior is provided with the transparent culture vessel that matches with described at least two pack support inboards by the triangular structure of the movable switching at least on one side of bottom girder (5) and at least the first curb girder (1) and second curb girder (2) formation.
2. culture apparatus according to claim 1, it is characterized in that: described first curb girder (1) and described bottom girder (5) are for fixedlying connected, described second curb girder (2) and described bottom girder (5) are flexible connection for being rotationally connected between described second curb girder (2) and described first curb girder (1).
3. culture apparatus according to claim 2, it is characterized in that: described transparent culture vessel is tubular plastics bag (11), and described plastics bag (11) top is provided with hot pressing broadside (10), and described hot pressing broadside (10) is provided with at least one suppending hole (14).
4. according to claim 1 or 2 or 3 described culture apparatuses, it is characterized in that: described at least two pack supports are arranged in array.
5. culture apparatus according to claim 4 is characterized in that: described plastics bag (11) is provided with outer catheter (12) and/or detects mouthful (13).
6. culture apparatus according to claim 4, it is characterized in that: the bottom of described plastics bag (11) is provided with parallel gas feed mechanism (20).
7. culture apparatus according to claim 4, it is characterized in that: described culture apparatus also comprises the mixing pit that links to each other with described transparent culture vessel by pipeline and pump (29).
8. culture apparatus according to claim 7, it is characterized in that: described culture apparatus comprises that two groups or more inside is provided with the body of transparent culture vessel, is provided with a mixing pit between the two adjacent groups body at least.
9. culture apparatus according to claim 1, it is characterized in that: described silk screen comprises the first side silk screen (3) that connects described at least two pack supports first curb girders (1), the second side silk screen (4) that connects described at least two pack supports second curb girders (2), and the bottom surface silk screen (6) that connects at least two pack support bottom girders (5).
10. culture apparatus according to claim 9, it is characterized in that: the bottom mesh-density of the described first side silk screen (3) and the second side silk screen (4) is greater than upper grid density.
11. culture apparatus according to claim 1 is characterized in that: flexibly connect the outside of holding at described bottom girder (5) and second curb girder (2), also be provided with locating part.
12. a photosynthetic little algae biological culture Unit Installation method comprises the steps:
Make auxiliary mould, on mechanically resistant material, offer two grooves that match with curb girder and/or bottom girder width dimensions according to the distance of two adjacent groups support;
The assembling support, first curb girder and bottom girder installation combination is fixing, and will be rotationally connected between second curb girder and the bottom girder;
The assembling body uses auxiliary mould, and first curb girder and bottom girder that at least two groups are fixed as one are formed array; Lay the bottom surface silk screen at each bottom girder, and after inserting silk screen bottom, first side high spot in the slit of locating on silk screen long limit in bottom surface, use auxiliary mould with working fastening between the first side silk screen and first curb girder;
Transparent culture vessel is installed, transparent culture vessel is suspended on the prominence at silk screen top, first side; After inserting silk screen bottom, second side high spot in the slit of locating on silk screen long limit in bottom surface, second curb girder is arranged on the periphery of the second side silk screen, and buckles the active connection on first curb girder and the second curb girder top.
13. installation method according to claim 12 is characterized in that: described method also is included in described bottom girder and second curb girder flexibly connects the step that the outer upper of holding arranges locating part.
CN201110446616.8A 2011-12-28 2011-12-28 Photosynthetic microbial culture apparatus and mounting method thereof Active CN103184140B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201110446616.8A CN103184140B (en) 2011-12-28 2011-12-28 Photosynthetic microbial culture apparatus and mounting method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201110446616.8A CN103184140B (en) 2011-12-28 2011-12-28 Photosynthetic microbial culture apparatus and mounting method thereof

Publications (2)

Publication Number Publication Date
CN103184140A true CN103184140A (en) 2013-07-03
CN103184140B CN103184140B (en) 2014-09-17

Family

ID=48675591

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201110446616.8A Active CN103184140B (en) 2011-12-28 2011-12-28 Photosynthetic microbial culture apparatus and mounting method thereof

Country Status (1)

Country Link
CN (1) CN103184140B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109251839A (en) * 2017-07-14 2019-01-22 中国石油化工股份有限公司 Utilize the device and method of sunlight culture photosynthetic microorganism
CN109536383A (en) * 2018-08-31 2019-03-29 雷云飞 A kind of activity of microalgae cytotrophy repairs the production line of liquid

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1145409A (en) * 1995-09-12 1997-03-19 广东省农业科学院果树研究所 Tissue culture method using polypropylene film bag as tissue culture packing vessel
CN201062270Y (en) * 2007-06-07 2008-05-21 邹宁 Industrialized scale economic optical bioreactor
CN101709264A (en) * 2009-06-19 2010-05-19 新奥科技发展有限公司 Optical bioreactor

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1145409A (en) * 1995-09-12 1997-03-19 广东省农业科学院果树研究所 Tissue culture method using polypropylene film bag as tissue culture packing vessel
CN201062270Y (en) * 2007-06-07 2008-05-21 邹宁 Industrialized scale economic optical bioreactor
CN101709264A (en) * 2009-06-19 2010-05-19 新奥科技发展有限公司 Optical bioreactor

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109251839A (en) * 2017-07-14 2019-01-22 中国石油化工股份有限公司 Utilize the device and method of sunlight culture photosynthetic microorganism
CN109251839B (en) * 2017-07-14 2021-08-06 中国石油化工股份有限公司 Device and method for culturing photosynthetic microorganisms by using sunlight
CN109536383A (en) * 2018-08-31 2019-03-29 雷云飞 A kind of activity of microalgae cytotrophy repairs the production line of liquid

Also Published As

Publication number Publication date
CN103184140B (en) 2014-09-17

Similar Documents

Publication Publication Date Title
CN101838606B (en) Airlift loop bioreactor through microalgae photoautotrophic-photoheterotrophic coupling for carbon emission reduction in sewage treatment
KR101148194B1 (en) Photobioreactor with Transparent Film
KR102043623B1 (en) Photobioreactors for algal farming and algal farming systems
CN102477397B (en) Flow guide device
WO2007098150A2 (en) Photobioreactor and uses therefor
US20170145361A1 (en) Bioreactor with separate co2 supply
CN1317379C (en) Pipeline photobioreactor for scale culture of microalgae
CN101748054B (en) Photobioreactor for cultivating microalgae
CN103039341B (en) Intensive resource recovering plant factory
CN101709264A (en) Optical bioreactor
CN101613664B (en) Air-lift photo-bioreactor
CN103966074A (en) Box-type photobioreactor for microalgae immobilization culture
CN201442951U (en) Airlift type photo-bioreactor
CN104988059B (en) A kind of bioreactor for algal culture
CN103184140B (en) Photosynthetic microbial culture apparatus and mounting method thereof
CN201424476Y (en) Photobioreactor
CN201729830U (en) Sealed pipeline culturing device for bait microalgae
CN102086437B (en) Thin film hanging-bag type Micro Algae bioreactor and preparation method thereof
CN100338205C (en) Photo bio reactor device of micro algae culture
CN2800737Y (en) Plant culturing box
CN202265566U (en) Photo reactor for cultivating photosynthetic microoganisms
CN205671189U (en) A kind of novel crop hydroponic device
WO2011013104A1 (en) Low-cost photobioreactor for microalgae cultivation
CN102344888B (en) Circulating type photobioreactor
CN201395597Y (en) Device for fast cultivating microalgae

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant