CN1145409A - Tissue culture method using polypropylene film bag as tissue culture packing vessel - Google Patents
Tissue culture method using polypropylene film bag as tissue culture packing vessel Download PDFInfo
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- CN1145409A CN1145409A CN 95114206 CN95114206A CN1145409A CN 1145409 A CN1145409 A CN 1145409A CN 95114206 CN95114206 CN 95114206 CN 95114206 A CN95114206 A CN 95114206A CN 1145409 A CN1145409 A CN 1145409A
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- tissue culture
- polypropylene film
- film bag
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- sack
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Abstract
A tissue culture method in which polypropylene film bag is used as tussue culture container other than glass bottle features that a holding device is used to clamp the mouthes of 6-10 said bags that has contained medium and to make them stand up; 2-6 holding devices are put in a big polypropylene film bag and sterilized, inoculation is conducted for each bag; and the mouthes of all the bags are sealed. Its advantages include low cost, small size, good sealing performance and high survival rate. The method is suitable to culture seedlings.
Description
The present invention relates to the tissue culture method of plant tissue culture seedling-growing method, particularly a kind of using polypropylene film bag as tissue culture packing vessel, be suitable for plant and grow seedlings in enormous quantities.
The personnel that are engaged in plant cultivation work know that tissue culture is the extensive method for culturing seedlings of a kind of batch production that grows up the beginning of the eighties, and are especially successful with banana seedlings, orchid seedling mass production.Initial tissue culture grows up with the test tube microorganism, because the test tube cross section is little, is unfavorable for a large amount of breedings and the growth of plant tissue culture, so the production cost height, and efficient is low.Afterwards, people used vial instead and made the tissue culture device, had improved productivity greatly, had realized industrial seedling rearing.Yet, confirm through production practice, use glass and also have the following defective: the one, weight is big and effective weight is little, heavily about 200 grams of empty bottle, and seedling and substratum have only 30 grams, effective weight less than 15%, and the carrying of vial has become the manual work of a burdensome; The 2nd, the cost height, about 0.4～0.5 yuan of the price of each vial, purchasing the bottle expense for the first time needs 30,000 yuan at least, and damaged easily, constantly needs to replenish; The 3rd, clean and detoxicating expense height; The 4th, the shared volume of vial is bigger, has increased the expense of depositing and selling transportation.External some testing laboratory adopted polypropylene plastic box to make the tissue culture device, though cracky not, but still exist and the similar problem of vial, and rate of utilization is low repeatedly, the cost height.
The objective of the invention is to overcome the deficiency of prior art, provide that a kind of production cost is low, productivity is high, operate and make things convenient for the tissue cultivating method of the using polypropylene film bag as tissue culture packing vessel of selling easily.
Task of the present invention is achieved in that and comprises prepared culture medium is put into culture vessel, being positioned in the damp and hot autoclave sterilization container through 120 ℃ of sterilization back taking-ups in 20～30 minutes allows it staticly condense to substratum again, on Bechtop, inoculate then, inoculation finishes culture vessel is sealed, be put at last and cultivate culturing room's cultivation, its improvements are to make the culture bag packaging container with polypropylene film bag, after putting into substratum, can press from both sides with one and will live its sack, and make it the upright holding frame of placing and be one group with 6～10 bags and be clamped together, the polypropylene film sack of then 2～6 holding frames being put into an opening is sent into damp and hot high-pressure sterilizer again and is sterilized, sealing the whole bag of sack after the sterilization leaves standstill to substratum and condenses, on a clean worktable, substratum is taken out inoculation subsequently, and will insert the polypropylene film bag sack good seal of culture.
The compared with the prior art following advantage of the present invention.
1, effective weight is little, reduces carrying labour intensity greatly.
2, compare and to reduce cost greatly with vial behind the employing polypropylene film bag.
3, the polypropylene tool printing opacity ventilation property good than vial, culture is than viridescent, the stalwartness of glass.
4, it is few to account for volume, and it is little to consume energy, and disinfection cost is low, as the horizontal sterilizing-cabinet of 60cm, and 400 vials of can sterilizing, and sack can be sterilized 1000 bags, cost dropped to 2 minutes/bag from 5 minutes/bottle.Culturing room saves nearly 3 times, and traffic capacity increases by 500.Can transport 90-100 ten thousand young plants with 5 tons of lorries of bag, and bottle can only be transported 14-20 ten thousand young plants.
5, polypropylene film conducts heat well, helps culture and does sth. in advance hardening, makes it more to adapt to natural temperature, heels in the surviving rate height.
6, because good sealing effect is not deposited bottle cap produces gas leakage when expanding with heat and contract with cold problem, no late stage of culture is polluted.
When enforcement is of the present invention, the size and the thickness of polypropylene film tissue culture packing bag do not have very strict requirement, can adopt the polypropylene film of 0.01～0.05 millimeter thickness to make, its size dimension is as the criterion with handled easily, generally with its width not big or small 150 millimeters for well, adopt 110 * 140 millimeters poly-two alkene bag films proper.The edge of bag must seal, and bag can not have small opening, to prevent the substratum seepage and to produce the later stage pollution.Described holding frame can adopt as depicted in figs. 1 and 2 folder mouth at last and trilateral and trapezoidal clamp structure that the end is flat, its width and height are to be a bit larger tham and to be slightly smaller than the width of culture bag pack and highly for well, but the folder mouth thickness of holding frame is proper with 6～10 bags of clamping, too big, the bag of clamping is more, be awkward, too smallly need more holding frame again.Holding frame can adopt stainless steel substrates, galvanized sheet iron, aluminium flake or Stainless Steel Wire to make.
Fig. 1 is the oblique drawing of the used holding frame of the present invention.
Fig. 2 is the oblique drawing of used another structure of holding frame of the present invention.
Fig. 3 is positioned over state graph on the holding frame for the polypropylene film bag of having put into substratum.
In the accompanying drawing, (1) for having adorned the polypropylene film bag of substratum, (2) are holding frame.
Embodiment: prepared culture medium solution is injected polypropylene film bag (1), then 8 bags are placed (2) on the holding frame, again 4 holding frames are put into a big polypropylene film bag, and allow its opening open to put into damp and hot high-pressure sterilizer, after 120 ℃ of sterilizations in 25 minutes, take out, seal sack, allow it leave standstill and condense until substratum.Subsequently sack is moved on the Bechtop that starts, opened sack, the pouch substratum is taken out inoculation, then will connect the sack good seal of the pouch of kind, they are put on the culturing rack uprightly sequence at last with the plastics film sealing machine.
The present invention is applicable to that plant grows seedlings in enormous quantities.
Claims (3)
1, a kind of tissue culture method of using polypropylene film bag as tissue culture packing vessel, comprise prepared culture medium is put into culture vessel, being positioned in the damp and hot autoclave sterilization container through 120 ℃ of sterilization back taking-ups in 20～30 minutes allows it staticly condense to substratum again, on Bechtop, inoculate then, inoculation finishes culture vessel is sealed, be put at last and cultivate culturing room's cultivation, it is characterized in that with the polypropylene film bag as tissue culture packing bag, the holding frame that can clamp its sack and make it uprightly to place with one after putting into substratum is one group with 6～10 bags and is clamped together, the polypropylene film sack of then 2～6 holding frames being put into an opening is put into damp and hot high-pressure sterilizer again and is sterilized, after finishing, sterilization seals sack, taking-up allows it leave standstill to substratum to condense, on a clean worktable, substratum is taken out inoculation, will insert the polypropylene film bag sack of culture at last and seal.
2, tissue culture method according to claim 1 is characterized in that described holding frame is that a folder is mouthful at last and trilateral or trapezoidal clamp structure that the end is flat.
3, tissue culture method according to claim 2 is characterized in that holding frame adopts stainless steel substrates, galvanized sheet iron, aluminium flake or Stainless Steel Wire to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95114206A CN1056408C (en) | 1995-09-12 | 1995-09-12 | Tissue culture method using polypropylene film bag as tissue culture packing vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95114206A CN1056408C (en) | 1995-09-12 | 1995-09-12 | Tissue culture method using polypropylene film bag as tissue culture packing vessel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1145409A true CN1145409A (en) | 1997-03-19 |
| CN1056408C CN1056408C (en) | 2000-09-13 |
Family
ID=5080245
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95114206A Expired - Fee Related CN1056408C (en) | 1995-09-12 | 1995-09-12 | Tissue culture method using polypropylene film bag as tissue culture packing vessel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1056408C (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184140A (en) * | 2011-12-28 | 2013-07-03 | 新奥科技发展有限公司 | Photosynthetic microbial culture apparatus and mounting method thereof |
| CN105638467A (en) * | 2016-01-06 | 2016-06-08 | 南平市华科生物科技有限公司 | Method for cultivating anoectochilus formosanus by using culture bag |
| CN108357763A (en) * | 2018-02-13 | 2018-08-03 | 京东方科技集团股份有限公司 | A kind of biological sheet storage device |
| CN108601329A (en) * | 2015-12-28 | 2018-09-28 | 麒麟株式会社 | The mass production method and extensive capital equipment of plant and the culture bag that they are used |
| CN109153953A (en) * | 2016-05-20 | 2019-01-04 | 康宁股份有限公司 | Conical flask with lining |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1045602A (en) * | 1989-03-08 | 1990-09-26 | 三井石油化学工业株式会社 | The production method of tissue culture method of plant and device thereof and meta-bolites |
-
1995
- 1995-09-12 CN CN95114206A patent/CN1056408C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184140A (en) * | 2011-12-28 | 2013-07-03 | 新奥科技发展有限公司 | Photosynthetic microbial culture apparatus and mounting method thereof |
| CN103184140B (en) * | 2011-12-28 | 2014-09-17 | 新奥科技发展有限公司 | Photosynthetic microbial culture apparatus and mounting method thereof |
| CN108601329A (en) * | 2015-12-28 | 2018-09-28 | 麒麟株式会社 | The mass production method and extensive capital equipment of plant and the culture bag that they are used |
| CN105638467A (en) * | 2016-01-06 | 2016-06-08 | 南平市华科生物科技有限公司 | Method for cultivating anoectochilus formosanus by using culture bag |
| CN109153953A (en) * | 2016-05-20 | 2019-01-04 | 康宁股份有限公司 | Conical flask with lining |
| US11136540B2 (en) | 2016-05-20 | 2021-10-05 | Corning Incorporated | Erlenmeyer flask assemblies with liners |
| CN108357763A (en) * | 2018-02-13 | 2018-08-03 | 京东方科技集团股份有限公司 | A kind of biological sheet storage device |
| US11174094B2 (en) | 2018-02-13 | 2021-11-16 | Boe Technology Group Co., Ltd. | Biological sheet storage device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1056408C (en) | 2000-09-13 |
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