CN1150885A - Technology for tissue culture and quick breeding of sprout using film bag - Google Patents
Technology for tissue culture and quick breeding of sprout using film bag Download PDFInfo
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- CN1150885A CN1150885A CN 95112559 CN95112559A CN1150885A CN 1150885 A CN1150885 A CN 1150885A CN 95112559 CN95112559 CN 95112559 CN 95112559 A CN95112559 A CN 95112559A CN 1150885 A CN1150885 A CN 1150885A
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Abstract
A tissue culture technique for quick breeding of seedlings with film includes such steps as loading culture medium in bag made of polypropylene film having 0.04-0.07 cm in thickness and 90-100% in light transmission rate, sealing its folded mouth, high-pressure (1.0-1.3 Kg/sq.cm) sterilization for 20-30 min, inoculation, sealing and culturing, and features less possessed space, easy long-distance transportation and low cost.
Description
The present invention relates to a kind of technology of utilizing the tissue cultivating and seedling of seedling, specifically refer to a kind of method of utilizing bag film to carry out the seedling tissue-culturing quick-propagation.
Continuous progress along with society, agrotechnique is also developed thereupon fast, and utilize the tissue cultivating and seedling technology of plant, also obtain bigger development, grasped by many research and production units in recent years, and developed into scale commercial production in batches, obtained bigger economic benefit, the present various tissue cultivating and seedling technology of utilizing, the container of the tissue culture that is adopted generally all adopts vial, ampere bottle as routine adopts this culture vessel, though have fixing culture space, and the advantage that is easy to manage, but because the culture vessel that adopted is a vial, thereby it is big to cause there is the space that blake bottle occupies, and is unfavorable for extensive cultivation; Transport difficulty is strong, and its empty bottle and seedling bottle all will need a large amount of transport spaces when transportation, will bring bigger transportation loss simultaneously; The cultivation cost is higher, only spends in the cleaning of vial just need expend great amount of manpower and expense.
In order to address the above problem, agrotechnique worker had once carried out a large amount of improvement research and had explored, but owing to fail the selection of effective breakthrough group culture container, thereby only be that the culture medium prescription in above-mentioned technology and the aspects such as environmental condition of cultivation are improved, thereby fail the existing in prior technology defective is done the improvement of essence.
Purpose of the present invention just is to provide a kind of method of utilizing bag film to carry out the seedling tissue-culturing quick-propagation, to pass through the employing polymeric film pouch that cost is low, in light weight, volume is little as tissue culture vessel, to replace existing vial, thereby make it in function that keeps prior art preferably and advantage, have take up space little, be beneficial to large-scale production, particularly long-distance transport of convenient transportation, the advantage that reduces production costs has effectively overcome the deficiencies in the prior art preferably.
The objective of the invention is to realize in the following manner: a kind of method of utilizing bag film to carry out the seedling tissue-culturing quick-propagation, mainly be that utilization group culture container and medium carry out Plant Tissue Breeding in suitable temperature and illumination, it is characterized in that above-mentioned group of culture container is that the employing wall thickness is 0.04mm～0.07mm, light permeable rate is the polypropylene film bag of 90-100, its group training technology is: the medium of technology preparation routinely of packing in the polypropylene film bag of selecting for use, after fold closure (non-tight), insert high-pressure sterilizing pot, under the pressure of 1.0～1.3 kg/cm, sterilized 20～30 minutes, taking out the back inoculates with conventional method under germ-free condition, and seal with sealing machine, then the culture bag of good seal is put into culturing room and cultivate.
Adopt the method for utilizing bag film to carry out the seedling tissue-culturing quick-propagation of the present invention, owing to adopted cost is low, in light weight, volume is little polymeric film pouch as tissue culture vessel, to replace existing vial, thereby make it in function that keeps prior art preferably and advantage, have:
1, the space that occupies of the bag film of bag training usefulness lack 2/3 than vial, and promptly the unit amount of storing of flower seed plantlet and amount of sterilization are for having 3 times of method now, and the production capacity of culturing room of unit is 3 times of prior art;
2, the weight of bag training seedling only is 1/8 of bottled seedling, and shared volume is 1/3 of a bottled seedling, and transport capacity is 30 times of bottled seedling, can more help the remote popularization of good seed;
3, removed the link of cleaning vial from, reduce vial and stacked the place, if clean with artificial, the cleaning charge of each vial is about 0.10 yuan with (comprise cleaning agent, water, artificial etc.), and only 0.03 yuan of each bag film, as produce seedling 2,000 ten thousand strains per year, then only this can save many 100,000 yuan;
4, remove the warehousing storage of vial from, as stack 10000 vials and need 15-20 cubic meter space approximately, and the bag film of same quantity only needs 0.1 cubic metre space, and access is convenient, do not have the problem of glass fragmentation.
Therefore adopt bag film to carry out the seedling tissue culture, have take up space little, be beneficial to large-scale production, particularly long-distance transport of convenient transportation, the advantage that reduces production costs has effectively overcome the deficiencies in the prior art preferably.
Describe the present invention below in conjunction with embodiment.
A kind of method of utilizing bag film to carry out the seedling tissue-culturing quick-propagation, mainly be that utilization group culture container and medium carry out Plant Tissue Breeding in suitable temperature and illumination, this group culture container is that the employing wall thickness is 0.04mm～0.07mm, light permeable rate is the polypropylene film bag of 90-100, its tissue culture technology is: the medium of technology preparation routinely of packing in bag film, after fold closure (non-tight), insert high-pressure sterilizing pot, under the pressure of 1.0～1.3 kg/cm, sterilized 20～30 minutes, taking out the back inoculates with conventional method under germ-free condition, and seal with sealing machine, then the culture bag of good seal is put into culturing room and cultivate.
Carry out in the technology that seedling cultivates at the existing plant tissue that utilizes, the prescription of its tissue culture medium (TCM), to environmental conditions such as the processing of its vial culture vessel and the temperature in incubation, humidity and light application times, carried out big quantity research, and obtained preferably and achieved, but because people are to having become intrinsic notion to vial as culture vessel, and since vial itself intrinsic defective, caused above-mentioned this advanced person's sapling multiplication technology to be difficult to large-scale production, thereby its large-area popularization is subjected to certain limitation.
The present invention is exactly when making full use of the various advantages of prior art, the bold polyacrylic film bag of selecting for use, replacing existing vial, and be equipped with the unique and effective sterilization process, make it overcome the deficiencies in the prior art preferably as tissue culture vessel.
Embodiment:
The employing wall thickness is that 0.05mm, light permeable rate are 90, specification is that 110 * 130 millimeters polypropylene film bag is the group culture container, the MS medium of in bag film, packing into, with the folding back of above-mentioned bag film clamp seal (non-tight) after, insert in the high-pressure sterilizing pot, sterilization is 25 minutes under the pressure of 1.2 kg/cm, take out the back and in sterile board, inoculate, and seal, then the culture bag of good seal is put into culturing room and cultivate with commercially available sealing machine with conventional method.The thatch culture medium prescription of inducing as the banana test-tube plantlet is: MS+5mg/l (6-BA)+1mg/l (NAA)+5% sucrose, PH5.8.The condition of culture of culturing room is: 25～28 ℃ of temperature, illumination every day 12 hours, intensity of illumination is 1500LX, cultivate about 20 days promptly switchable.
Claims (2)
1, a kind of method of utilizing bag film to carry out the seedling tissue-culturing quick-propagation, mainly be that utilization group culture container and medium carry out Plant Tissue Breeding in suitable temperature and illumination, it is characterized in that above-mentioned group of culture container is that the employing wall thickness is that 0.04mm～0.07mm, light permeable rate are the polypropylene film bag of 90-100.
2, the method for utilizing bag film to carry out the seedling tissue-culturing quick-propagation according to claim 1, it is characterized in that its tissue culture technology is: the medium of technology preparation routinely of in the polypropylene film bag of selecting for use, packing into, after fold closure (non-tight), insert in the high-pressure sterilizing pot, under the pressure of 1.0～1.3 kg/cm, sterilized 20～30 minutes, taking out the back inoculates with conventional method under germ-free condition, and seal with sealing machine, then the culture bag of good seal is put into culturing room and cultivate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95112559 CN1150885A (en) | 1995-11-21 | 1995-11-21 | Technology for tissue culture and quick breeding of sprout using film bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95112559 CN1150885A (en) | 1995-11-21 | 1995-11-21 | Technology for tissue culture and quick breeding of sprout using film bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1150885A true CN1150885A (en) | 1997-06-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95112559 Pending CN1150885A (en) | 1995-11-21 | 1995-11-21 | Technology for tissue culture and quick breeding of sprout using film bag |
Country Status (1)
| Country | Link |
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| CN (1) | CN1150885A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742508A (en) * | 2012-08-08 | 2012-10-24 | 昆明琪鼎权生物科技有限公司 | Method for utilizing tissue culture bag to simulate ecology to perform plant tissue culture |
| CN105638467A (en) * | 2016-01-06 | 2016-06-08 | 南平市华科生物科技有限公司 | Method for cultivating anoectochilus formosanus by using culture bag |
| CN108601329A (en) * | 2015-12-28 | 2018-09-28 | 麒麟株式会社 | The mass production method and extensive capital equipment of plant and the culture bag that they are used |
| CN112772412A (en) * | 2020-12-30 | 2021-05-11 | 吴有光 | Method for performing open type plant tissue culture and rapid propagation by utilizing self-standing self-sealing film bag |
-
1995
- 1995-11-21 CN CN 95112559 patent/CN1150885A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742508A (en) * | 2012-08-08 | 2012-10-24 | 昆明琪鼎权生物科技有限公司 | Method for utilizing tissue culture bag to simulate ecology to perform plant tissue culture |
| CN108601329A (en) * | 2015-12-28 | 2018-09-28 | 麒麟株式会社 | The mass production method and extensive capital equipment of plant and the culture bag that they are used |
| CN105638467A (en) * | 2016-01-06 | 2016-06-08 | 南平市华科生物科技有限公司 | Method for cultivating anoectochilus formosanus by using culture bag |
| CN112772412A (en) * | 2020-12-30 | 2021-05-11 | 吴有光 | Method for performing open type plant tissue culture and rapid propagation by utilizing self-standing self-sealing film bag |
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