CN2571767Y

CN2571767Y - Bag shape container for tissue culture

Info

Publication number: CN2571767Y
Application number: CN 02257668
Authority: CN
Inventors: 梁珍海; 蒋泽平; 李玉巧; 倪竞德; 吴钢; 刘根林
Original assignee: JIANGSU LUBAO PLANT MICROBREEDING CO
Current assignee: JIANGSU LUBAO PLANT MICROBREEDING CO
Priority date: 2002-10-09
Filing date: 2002-10-09
Publication date: 2003-09-10
Anticipated expiration: 2012-10-09

Abstract

The utility model relates to a bag-shaped container for culturing a tissue. The utility model comprises a bag-shaped container (1) made of transparent plastic thin film. A window is arranged on the bag-shaped container and is covered by an aseptic filtering film (2). The bag-shaped container for culturing a tissue of the utility model can adjust air permeability and is suitable for the growth of aseptic seedlings of each stage for culturing a plant tissue. The concentration of CO 2 in the bag is relatively lower, the relative humidity is low, and the concentration of O2 is relatively higher, which is good for the growth of the aseptic seedlings. The survival rate of the transplantation of the aseptic seedlings is high. The bag-shaped container for culturing a tissue of the utility model has the advantages of little one-off investment, small volume, convenient transportation and low cost.

Description

A kind of tissue culture bag-like container

One, technical field

The utility model relates to a kind of tissue culture vessel.

Two, background technology

Appliable plant tissue culture technique culturing plants aseptic seedling, widespread use now be simple glass or plastic containers such as wide-necked bottle, culture dish, though these containers can satisfy the plant tissue culture requirement, but because one to be that a large amount of culturing bottles accounts for volume big, weight is big, disposable having high input, cost height; The 2nd, the expense of cleaning, autoclaving and illumination cultivation is big, efficient is low, and the 3rd, transportation is inconvenient, expense is big.

Chinese patent 95114206.2 (Granted publication CN1056408C), though can satisfy light transmission, sterility, it is little to account for volume, is convenient to transportation, requirement such as anti fouling performance during inoculation, but because CO ₂/ O ₂Exchange is few, and situations such as the interior relative humidity height of bag only are applicable to that growth cycle is short, the herbal growth of anti-shade; Some xylophytas, particularly some good gas plants are poor growth in this bag, produces large quantities of glass seedlings, and little transplantation of seedlings is difficult to survive.

Three, summary of the invention

The utility model is the deficiency that overcomes prior art, provides a kind of production cost low, the production efficiency height, and convenient transportation is fit to the various plants tissue culture and produces, and can regulate breather hole size, quantity, makes a bag inside and outside CO ₂/ O ₂Exchange keeps bag interior than low relative humidity, aseptic seedling robust growth, the tissue culture vessel that transplanting survival rate is high.Its disposable less investment, operation are easily transported just, and less energy consumption in the production can be saved great amount of cost, and group training industrialization, large-scale production are promoted.

The technical solution of the utility model is as follows:

A kind of tissue culture bag-like container, it is the bag-like container that transparent plastic film is made, have window on the bag-like container, window covers with aseptic filter membrane, transparent plastic film can be transparent polypropylene film or polyethylene, polyester film, its thickness can be 0.03～0.05mm, and aseptic filter membrane can stick on the window bag-like container all around with multi-purpose adhesive.Aseptic filter membrane is that a kind of permission gas such as air, oxygen, carbon dioxide gas, water vapour pass through, and bacterium, the intransitable film of fungi.

Above-mentioned tissue culture bag-like container can have 2-4 window, and window can be square or circular, and is preferably circular;

Above-mentioned tissue culture bag-like container can be a different shape, as square, rectangle, trapezoidal or trapezoidal, and the rectangle of 100～150mm * 120～150mm preferably, the diameter of the circular window on it can be 2～4mm.

Tissue culture bag-like container of the present utility model can 120 ℃ of sterilizations behind the can substratum, and the inoculation plant seedlings after the sealing of plastics film sealing machine, is put into culturing room and cultivated in substratum under aseptic condition.Because have window on the bag-like container, window covers with aseptic filter membrane, therefore both guaranteed aseptic in the tissue culture bag-like container gas in the bag to be exchanged with the external world, make a bag interior CO ₂Concentration reduce O ₂Content increase, the relative humidity in the bag reduces, and according to different floristics and different vegetative period, can select the size and the quantity of window, has guaranteed the seedling robust growth of bag-like container, the transplanting survival rate height.

The utility model is advantage compared with the prior art:

1. the design's bag-type container can be regulated Gas permeability, is fit to the growth of each stage aseptic seedling of plant tissue culture.

2. more than existing design and more consider a bag implants required CO that grows ₂/ O ₂Gaseous interchange and lower relative humidity in order to the strong shape of aseptic seedling growth, improve the aseptic seedling transplanting survival rate greatly.

3. disposable input is little, and volume is little, convenient transportation, and cost is low.

Four, description of drawings

Fig. 1 is the synoptic diagram that ladder weave is cultivated bag-like container, and it is trapezoidal, and bag-like container antetheca and rear wall respectively have a square window, post aseptic filter membrane on the window;

Fig. 2 is the synoptic diagram of square tissue culture bag-like container, and its antetheca and rear wall respectively have a square window, post aseptic filter membrane on the window;

Fig. 3 is the synoptic diagram of square tissue culture bag-like container, and its antetheca and rear wall respectively have the window of two circles, posts aseptic filter membrane on the window.

1 is Plastic film Bag among the figure; 2 is aseptic filter membrane.

Five, embodiment

Embodiment 1:

A kind of tissue culture bag-like container of the present utility model, as shown in Figure 3, it is the rectangle bag 1 of 120 * 150mm of being made by 0.03mm transparent polypropylene film, article three, limit sealing, a 120mm limit opening is apart from opening edge 40mm place, the window that it is 4mm that two bags of walls have two diameters, window covers with aseptic filter membrane 2 (Shanghai is sowed rich horticultural facility company and produced), sticks on the polypropylene film around the window with multi-purpose adhesive, and it is intact to reach sealing.

Sterilized 20～25 minutes at 120 ℃ after tissue culture bag-like container of the present utility model can being put into substratum, under aseptic condition, inoculate then, with the sealing of bag-like container opening edge, place culturing room to cultivate with plastic sealing machine.Gas can be by the outer exchange of aseptic filter membrane and bag in the bag in culturing process for tissue culture bag-like container of the present utility model, therefore CO in bag interior gas ₂Lower with the concentration ratio of water vapor, help the growth of seedling, make the strong shape of seedling growth, the transplanting survival rate height.

Claims

1. tissue culture bag-like container, it is the bag-like container (1) that transparent plastic film is made, and it is characterized in that having on the bag-like container window, window covers with aseptic filter membrane (2).

2. tissue culture vessel according to claim 1 is characterized in that having 2～4 windows.

3. tissue culture vessel according to claim 1 and 2 is characterized in that window is circular.

4. tissue culture vessel according to claim 1 is characterized in that the thick 0.03～0.05mm of transparent plastic film.

5. tissue culture vessel according to claim 1 is characterized in that this container is the rectangular bag-like container of 100～150mm * 120～150mm.

6. tissue culture vessel according to claim 5 is characterized in that this container has 2～4 circular windows, and the window diameter is 2～4mm.