CN102349445A - Method for producing tissue culture seedlings on assembly line with low cost - Google Patents
Method for producing tissue culture seedlings on assembly line with low cost Download PDFInfo
- Publication number
- CN102349445A CN102349445A CN2011102293817A CN201110229381A CN102349445A CN 102349445 A CN102349445 A CN 102349445A CN 2011102293817 A CN2011102293817 A CN 2011102293817A CN 201110229381 A CN201110229381 A CN 201110229381A CN 102349445 A CN102349445 A CN 102349445A
- Authority
- CN
- China
- Prior art keywords
- tissue culture
- bag
- test
- culture
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a method for producing tissue culture seedlings on an assembly line with low cost, which comprises the following steps of: preparing a culture medium; sub-packaging and sterilizing the culture medium; separating and inoculating an explant by sterile operation; and carrying out tissue culture and the like. In the invention, a polypropylene (PP) film bag replaces the traditional glass container, and an illumination source for tissue culture is improved. The PP film bag culture container adopted in the method has the advantages of good light permeability, good air permeability, low cost, light weight and great effective weight, and is more convenient to carry. By adopting the improved method, the production cost of the tissue culture seedlings is lowered, and the production efficiency is obviously improved.
Description
Technical field
The present invention relates to a kind of method of producing tissue cultivating seedling, particularly a kind of method of low-coat scale production tissue cultivating seedling.
Background technology
Plant Tissue Breeding is a plant aseptic culture technology; Be to have totipotent theory according to plant cell; Organ (like root, stem, leaf, stem apex, flower, the fruit etc.) tissue (like cambium, epidermis, cortex, marrow cell, endosperm etc.) or cell (like megaspore, microspore, somatic cell etc.) and the protoplast that utilize plant corpus to exsomatize; Under artificial conditions such as aseptic and suitable synthetic medium and illumination, temperature; Can induce callus, indefinite bud, adventive root, form complete plant at last.
But the employed glass culture vessel of traditional mode of production tissue cultivating seedling printing opacity gas permeability is poor, cost high, weight is big, effective weight is little, difficult, and is fragile.Production cost is higher, and production efficiency is lower, and the further improvement of low-coat scale production tissue cultivating seedling technology can be arranged.
Summary of the invention
The object of the present invention is to provide a kind of low-cost tissue culture vessel that is easy to promote, set up a kind of method for tissue culture of easy, low-cost, streamlined.
In order to realize the foregoing invention purpose, the technical scheme that the present invention adopts is following:
The present invention includes following steps:
(1) culture medium preparation:
(1) medicine that takes by weighing following weight fraction places beaker respectively:
KH
2PO
4 3.75
NH
4NO
3 36.43
KNO
3 41.94
CaCl
2·2H
2O 9.71
MgSO
4·7H
2O 8.17
After in each beaker, adding the doubly heavy dissolved in purified water of medicine gross weight 33-34 respectively, solution slowly is mixed in the bigger beaker, constantly stirs simultaneously according to listed each medicine sequencing, for use;
(2) take by weighing the medicine of following weight fraction, the medicine gross mass be in (1) medicine gross mass 1/22:
MnSO
4·4H
2O 10.8 ZnSO
4·7H
2O 4.17
H
3BO
3 3.00 KI 0.40
Na
2MoO
4·2H
2O 0.12 CoCl
2·6H
2O 0.012
CuSO
4·5H
2O 0.012 FeSO
4·7H
2O 13.47
Na
2-EDTA 18.07 nicotinic acid 0.24
Vitamin B6 0.24 vitamin B1 0.05
All the other are inositol for glycine 0.97
Above medicine is placed beaker, adds the dissolved in purified water of 1000 times of weights of medicine gross weight,
Slowly pour in the solution for preparing in (1), behind the stirring and evenly mixing, for use;
(3) compound concentration is 6-benzyl aminoadenine (6-BA) and methyl (NAA) solution of 0.2mg/ml;
(4) take by weighing the agar of 30 times of medicine gross weights in (2), 150 times sucrose is poured in the solution in (1) step and is stirred; Behind the ebuillition of heated; The 6-benzyl aminoadenine (6-BA) and methyl (NAA) solution that add (3) preparation of different volumes according to the test-tube plantlet of different plants; At last by medicine gross weight in (1); Every 4.53g constant volume dropwise splashes into the pH value to 5.8 that potassium hydroxide solution (1N) is regulated medium again to 1000mL;
(2) packing and sterilization:
Prepared culture medium is divided in the polypropylene film bag of packing into while hot, be fixed on the simple and easy framework made from iron wire with Small clamp and make it upright, put into the high-pressure sterilizing pot sterilization then, sterilization time keeps 18～20min to get final product at 121 ℃;
(3) sterile working:
(1) preparation of sterile working experiment utensil and material: comprise the aseptic process of superclean bench, sterilization both hands and operation tools such as cutter and tweezers;
(2) after sterilization finishes, simple and easy shelf is placed on the superclean bench, the arrangement bag film makes it can be upright, seals sack with the plastic bag sealing machine, and bag film leaves standstill to culture medium solidifying, and is for use;
(3) continuous productive process is carried out the separation and the inoculation of explant: culture bag, operation utensil, culture dish and the plastic bag sealing machine etc. that test-tube plantlet is housed are put into superclean bench after with bromogeramine or the sterilization of 75% alcohol wipe; No. 1 the tissue culture technology workman takes out test-tube plantlet in superclean bench from culture bag, puts on the aseptic filter paper, and the test-tube plantlet of treating breeding according to the breeding requirement of different test-tube plantlets with cutter cuts; Simultaneously No. 2 skilled workers cut off the bag film sealing part that can medium and medium have solidified, and the test-tube plantlet of the breeding of No. 1 tissue culture technology workman being cut with the tweezers of sterilize and cooling off sandwiches in the bag film; If stem tuber or the little insertion medium of stem section that No. 3 tissue culture technology workmans put into No. 2 tissue culture technology workmans are the petiole horizontal positioned; No. 4 tissue culture technology workmans seal No. 3 ready-made bag films of tissue culture technology workman and number with its bag films with the plastic bag sealing machine, are positioned on the culturing rack in the culturing room;
(4) carry out sterile working by (three) said step, the test-tube plantlet of bag film encapsulation is positioned over carries out tissue culture on the culturing rack in the culturing room, the lighting source of tissue culture adopts electricity-saving lamp, is preferably 11 watts electricity-saving lamp.
Compared with prior art, the invention has the beneficial effects as follows:
1, adopt polypropylene film bag as culture vessel, the printing opacity gas permeability that its tool is good than glass, culture is than viridescent, the stalwartness of traditional glass container.Polypropylene film conducts heat good, helps culture and refines seedling ahead of time, makes it more to adapt to natural temperature, and transplanting survival rate is high.
2, the traditional glass container weight is big and effective weight is little; About heavily about 240 grams of general empty bottle; And seedling and medium have only about 55 grams; Effective weight is about 23% of an empty bottle weight, and the carrying of vial has become the manual labor of a burdensome, transplants if tissue cultivating seedling will be transported into the greenhouse; Cost of transportation increases; And the shared volume of vial is bigger, puts into culturing room and cultivates, and has increased the expense of depositing.And polypropylene film bag is in light weight, has not only reduced cost of transportation, and has alleviated working strength of workers, and is also convenient during transplanting.
3, the traditional glass container cost is high, and the glass container of a 350ml needs about 0.8～1.0 yuan, and damaged easily, need constantly replenish, and the bag film cost is low only about 0.035 yuan/individual.In addition, it is high to use the traditional glass container to clean with the sterilization expense, and polypropylene film bag as container removed from cleaning charge with and sterilization take and also reduced.
4, the bag film light transmission is good, adds the improvement light source, uses electricity-saving lamp, in culturing room, if be placed on 1.0m * 2.0m culturing rack, only needs totally 44 watts electricity-saving lamp, and culture gets final product normal growth.
5, the tissue culture technology set up of the present invention is easy and be easy to grasp.The inoculation step of streamlined has improved operating efficiency, adopts this kind method not only to reduce the production cost of tissue cultivating seedling, and has improved the production efficiency of tissue cultivating seedling, compares with conventional method and has played the effect of getting twice the result with half the effort.
Embodiment
Below in conjunction with embodiment foregoing invention content of the present invention is described in further detail.
But this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment.Not breaking away under the above-mentioned technological thought situation of the present invention, according to ordinary skill knowledge and customary means, make various replacements and change, all should comprise within the scope of the invention.
Embodiment 1
Present embodiment is a culture medium preparation:
(1) takes by weighing KH
2PO
4170mg, NH
4NO
31650mg, KNO
31900mg, CaCl
22H
2O 440mg, MgSO
47H
2O 370mg places the beaker of 200mL respectively, add pure water 150 mL dissolving after, solution slowly is mixed in the beaker of 2000mL according to listed each medicine sequencing, constantly stir simultaneously, for use.
(2) take by weighing MnSO
44H
2O 22.3 mg, ZnSO
47H
2O 8.6 mg, H
3BO
36.2 mg,
KI 0.83 mg, Na
2MoO
42H
2O 0.25 mg, CoCl
26H
2O 0.025 mg, CuSO
45H
2O 0.025 mg, FeSO
47H
2O 27.8 mg, Na
2-EDTA 37.3 mg, nicotinic acid 0.5 mg, vitamin B6 0.5mg, vitamin B1 0.1mg; Glycine 2.0 mg, inositol 100 mg in the beaker of 500mL, add pure water 200 mL dissolving after; Slowly pour in the beaker of the 2000mL in 1, behind the stirring and evenly mixing, for use.
(3) compound concentration is 6-benzyl aminoadenine (6-BA) and methyl (NAA) solution of 0.2mg/ml;
(4) accurately taking by weighing agar 6g, sucrose 30g pours in the beaker of the 2000mL in 1 step and stirs; Behind the ebuillition of heated; The 6-benzyl aminoadenine (6-BA) and methyl (NAA) solution that add different volumes according to the test-tube plantlet of different plants; Add pure water to beaker scale 1000mL place at last, dropwise splash into the pH value to 5.8 that potassium hydroxide solution is regulated medium again.
Embodiment 2
Present embodiment is packing and sterilization
Prepared culture medium is divided in the polypropylene film bag of packing into while hot.Be fixed on the simple and easy framework made from iron wire with Small clamp and make it upright, put into the high-pressure sterilizing pot sterilization then, 121 ℃ of maintenance 20min sterilizes.
Embodiment 3
Present embodiment is sterile working
(1) preparation of sterile working experiment utensil and material:, open superclean bench and indoor uviol lamp, sterilization 20～30min with bromogeramine or 75% alcohol wipe superclean bench.Articles for use on the work top can not be placed too many or overlapping placement, in order to avoid reduce sterilization effect; With bromogeramine or 75% wipes of alcohol table top and sterilize both hands and test tools made, open the inoculation sterilizer, when temperature reaches 290 ℃～300 ℃ operation tool (cutter and tweezers) is put into about 2 minutes of sterilization, take out, be placed on the tool rack and cool off.
(2) after sterilization finishes, immediately simple and easy shelf is placed on the superclean bench, the arrangement bag film makes it can be upright, seals sack with the plastic bag sealing machine, and bag film leaves standstill to culture medium solidifying, and is for use.
(3) continuous productive process is carried out the separation and the inoculation of explant: culture bag, operation utensil, culture dish and the plastic bag sealing machine etc. that test-tube plantlet is housed are put into superclean bench after with bromogeramine or the sterilization of 75% alcohol wipe.No. 1 the tissue culture technology workman takes out test-tube plantlet in superclean bench from culture bag, puts on the aseptic filter paper, and the test-tube plantlet of treating breeding according to the breeding requirement of different test-tube plantlets with cutter cuts: if the stem section is cut to 1cm, if the stem tuber rip cutting is 1cm
3If petiole segment 1～1.2cm is if blade is cut into the square of 1cm * 1cm; Simultaneously No. 2 skilled workers cut off the bag film sealing part that can medium and medium have solidified, and the test-tube plantlet of the breeding of No. 1 tissue culture technology workman being cut with the tweezers of sterilize and cooling off sandwiches in the bag film; If stem tuber or the little insertion medium of stem section that No. 3 tissue culture technology workmans put into No. 2 tissue culture technology workmans are the petiole horizontal positioned; No. 4 tissue culture technology workmans seal with the plastic bag sealing machine No. 3 ready-made bag films of tissue culture technology workman (test-tube plantlet that breeding is arranged in the bag) with its bag film, write the test-tube plantlet numbering of cultivation, are positioned on the culturing rack of putting in the culturing room.
Embodiment 4
Present embodiment is a tissue culture
Sterile working method according to embodiment 3 introduces is carried out tissue culture; The test-tube plantlet of bag film encapsulation is positioned over carries out tissue culture on the culturing rack in the culturing room, the illumination light source improves: lighting source adopts 11 watts electricity-saving lamp to substitute former 40 watts fluorescent tube.
Embodiment 5
Present embodiment is the economic effect evaluation to the described technology of embodiment 1-4:
(1) 10,000 bottles of (traditional glass container 250ml) medium of preparation need 500 liters, with 3 yuan of medium component costs/rise calculating, need 1500 yuan.
Prepare 10,000 bags of (polypropylene film bag) medium and need 400L,, need 1200 yuan with 3 yuan of medium component costs/rise calculating.
(2) 10,000 bottles of (traditional glass container 350ml) medium of preparation calculate with the 50ml/ bottle, need 500 liters, and a workman can prepare 15 liters in one day, and 40 yuan/day of labour costs need 1340 yuan altogether.
Prepare 10,000 bags of (polypropylene film bag) medium, calculate with the 40ml/ bag, need 400 liters, a workman can prepare 15 liters in one day, and 40 yuan/day of labour costs need 1070 yuan altogether.
(3) 120L vertical pressure steam sterilizing pot 150 vials of once can sterilizing, and sack can be sterilized 200 bags.Equally once sterilization needs 12 degree electricity, and kilowatt-hour one yuan amounts to 12 yuan.Can sterilize in one day 15 liters medium of workman, 40 yuan/day of labour costs.10,000 vials of sterilizing, 1340 yuan of labour costs, the electricity charge consume 800 yuan, amount to 2140 yuan; 10,000 bag films of sterilizing, 1070 yuan of labour costs, the electricity charge consume 600 yuan, amount to 1670 yuan.
(4) vial seals 0.10 yuan of film/individual, and 10,000 bottles of medium need 1000 yuan.0.035 yuan of polypropylene film bag/individual, 350 yuan of 10,000 bag films.
(5) inoculation: every bag (bottle) inoculation 18 seedlings, 40 yuan/day of wage for workmen use vial, and bag film is used in 120 bottles of people inoculations in a day, can inoculate 200 bags, and inoculating 10,000 bottles of test-tube plantlets need labour cost 3334 yuan.Connect 10,000 bags of test-tube plantlets and need 2000 yuan of labour costs.
(6) if use vial, then need clean bottle.A workman washed 300 vials in one day, 40 yuan/day of wages, 1340 yuan of 10,000 bottles of need.
(7) the illumination cultivation electricity charge are adjusted: 1.0m * 2.0m culturing rack can be put 325 bag culture mediums; Because the bag film light transmission is good; Use 4 different electricity-saving lamps of power to amount to 44 watts; Tissue cultivating seedling gets final product normal cultured; Calculate like this, 10,000 bags of tissue cultivating seedling illumination cultivation 30 days, lighting hours was 10 hours in one day; Power consumption 410 degree, 410 yuan of the electricity charge (1 degree electricity is by 1 yuan of calculating); Equally, 1.0m * 2.0m culturing rack is placed 160 bottles of medium, needs three 40 watts of fluorescent tubes, power consumption 2268 degree, 2668 yuan of the electricity charge.
(8) totle drilling cost: adopt bag film to do culture vessel, the cost of producing 180,000 test-tube plantlets is 6700 yuan, and 0.0372 yuan of average every seedling cost adopts vial to do culture vessel, and the cost of producing 180,000 test-tube plantlets is 13322 yuan, 0.0744 yuan of average every seedling cost.Adopt bag film to do culture vessel, save production cost 6622 yuan, the every seedling cost of test-tube plantlet be vial do culture vessel 1/2nd.
(9) traffic expense: the lorry of load-carrying 1.0L, average 2 yuan/kilometer, a car can be adorned 20000 bags, and if adopt vial, a car is only adorned 7200 bottles.By one kilometer calculating of use 1.0L Freight Transport, 50,000 bags of test-tube plantlet traffic expense 5 yuans, 50,000 bottles of test-tube plantlets then need 14 yuan, practice thrift 9 yuan, and the cost of transportation that adopts bag film to do culture vessel is to adopt vial to make 35.7% of culture vessel cost of transportation.
In sum, employed polypropylene film bag culture vessel printing opacity good permeability among the present invention, cost is low, in light weight, effective weight big, carrying is simpler.Through technology of the present invention, make the tissue cultivating seedling production cost reduce, production efficiency significantly improves.
Claims (2)
1. method that low-cost streamlined is produced tissue cultivating seedling may further comprise the steps:
(1) culture medium preparation:
(1) medicine that takes by weighing following weight fraction places beaker respectively:
KH
2PO
4 3.75
NH
4NO
3 36.43
KNO
3 41.94
CaCl
2·2H
2O 9.71
MgSO
4·7H
2O 8.17
After in each beaker, adding the doubly heavy dissolved in purified water of medicine gross weight 33-34 respectively, solution slowly is mixed in the bigger beaker, constantly stirs simultaneously according to listed each medicine sequencing, for use;
(2) take by weighing the medicine of following weight fraction, the medicine gross mass be in (1) medicine gross mass 1/22:
MnSO
4·4H
2O 10.8 ZnSO
4·7H
2O 4.17
H
3BO
3 3.00 KI 0.40
Na
2MoO
4·2H
2O 0.12 CoCl
2·6H
2O 0.012
CuSO
4·5H
2O 0.012 FeSO
4·7H
2O 13.47
Na
2-EDTA 18.07 nicotinic acid 0.24
Vitamin B6 0.24 vitamin B1 0.05
All the other are inositol for glycine 0.97
Above medicine is placed beaker, adds the dissolved in purified water of 1000 times of weights of medicine gross weight,
Slowly pour in the solution for preparing in (1), behind the stirring and evenly mixing, for use;
(3) compound concentration is 6-benzyl aminoadenine (6-BA) and methyl (NAA) solution of 0.2mg/ml;
(4) take by weighing the agar of 30 times of medicine gross weights in (2), 150 times sucrose is poured in the solution in (1) step and is stirred; Behind the ebuillition of heated; The 6-benzyl aminoadenine (6-BA) and methyl (NAA) solution that add (3) preparation of different volumes according to the test-tube plantlet of different plants; At last by medicine gross weight in (1); Every 4.53g constant volume dropwise splashes into the pH value to 5.8 that potassium hydroxide solution (1N) is regulated medium again to 1000mL;
(2) packing and sterilization:
Prepared culture medium is divided in the polypropylene film bag of packing into while hot, be fixed on the simple and easy framework made from iron wire with Small clamp and make it upright, put into the high-pressure sterilizing pot sterilization then, sterilization time keeps 18～20min to get final product at 121 ℃;
(3) sterile working:
(1) preparation of sterile working experiment utensil and material: comprise the aseptic process of superclean bench, sterilization both hands and operation tools such as cutter and tweezers;
(2) after sterilization finishes, simple and easy shelf is placed on the superclean bench, the arrangement bag film makes it can be upright, seals sack with the plastic bag sealing machine, and bag film leaves standstill to culture medium solidifying, and is for use;
(3) continuous productive process is carried out the separation and the inoculation of explant: culture bag, operation utensil, culture dish and the plastic bag sealing machine etc. that test-tube plantlet is housed are put into superclean bench after with bromogeramine or the sterilization of 75% alcohol wipe; No. 1 the tissue culture technology workman takes out test-tube plantlet in superclean bench from culture bag, puts on the aseptic filter paper, and the test-tube plantlet of treating breeding according to the breeding requirement of different test-tube plantlets with cutter cuts; Simultaneously No. 2 skilled workers cut off the bag film sealing part that can medium and medium have solidified, and the test-tube plantlet of the breeding of No. 1 tissue culture technology workman being cut with the tweezers of sterilize and cooling off sandwiches in the bag film; If stem tuber or the little insertion medium of stem section that No. 3 tissue culture technology workmans put into No. 2 tissue culture technology workmans are the petiole horizontal positioned; No. 4 tissue culture technology workmans seal No. 3 ready-made bag films of tissue culture technology workman and number with its bag films with the plastic bag sealing machine, are positioned on the culturing rack in the culturing room;
(4) carry out sterile working by (three) said step, the test-tube plantlet of bag film encapsulation is positioned over carries out tissue culture on the culturing rack in the culturing room, the lighting source of tissue culture adopts electricity-saving lamp.
2. the method that a kind of low-cost streamlined according to claim 1 is produced tissue cultivating seedling, it is characterized in that: the lighting source of tissue culture adopts 11 watts electricity-saving lamp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110229381 CN102349445B (en) | 2011-08-11 | 2011-08-11 | Method for producing tissue culture seedlings on assembly line with low cost |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110229381 CN102349445B (en) | 2011-08-11 | 2011-08-11 | Method for producing tissue culture seedlings on assembly line with low cost |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102349445A true CN102349445A (en) | 2012-02-15 |
| CN102349445B CN102349445B (en) | 2013-02-06 |
Family
ID=45573174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110229381 Expired - Fee Related CN102349445B (en) | 2011-08-11 | 2011-08-11 | Method for producing tissue culture seedlings on assembly line with low cost |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102349445B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936579A (en) * | 2012-09-23 | 2013-02-20 | 四川省农业科学院园艺研究所 | Liquid culture medium for promoting fruit tree tissue culture seedling growth |
| CN105638467A (en) * | 2016-01-06 | 2016-06-08 | 南平市华科生物科技有限公司 | Method for cultivating anoectochilus formosanus by using culture bag |
| CN109076959A (en) * | 2018-08-31 | 2018-12-25 | 湖州德清玖沐农业科技有限公司 | A kind of generic plant tissue culture culture medium and preparation method |
| CN112167064A (en) * | 2020-11-11 | 2021-01-05 | 山东农业大学 | Tissue culture and rapid propagation method for salvia miltiorrhiza |
| CN112772412A (en) * | 2020-12-30 | 2021-05-11 | 吴有光 | Method for performing open type plant tissue culture and rapid propagation by utilizing self-standing self-sealing film bag |
| CN116458426A (en) * | 2023-03-20 | 2023-07-21 | 云浮市南药研究院 | Energy-saving simple plant tissue culture aseptic operation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2262801Y (en) * | 1996-01-10 | 1997-09-24 | 付翔 | Culture bag by tissue culture |
| CN1586169A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Poplar tissue cultivation quick breeding industrial producing plan controlled digital model |
-
2011
- 2011-08-11 CN CN 201110229381 patent/CN102349445B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2262801Y (en) * | 1996-01-10 | 1997-09-24 | 付翔 | Culture bag by tissue culture |
| CN1586169A (en) * | 2004-08-06 | 2005-03-02 | 上海光兆植物速生技术有限公司 | Poplar tissue cultivation quick breeding industrial producing plan controlled digital model |
Non-Patent Citations (3)
| Title |
|---|
| 吴国智等: "红掌组培苗工厂化生产技术及降低成本措施", 《天津农业科学》, vol. 16, no. 1, 31 December 2010 (2010-12-31), pages 123 - 126 * |
| 宿飞飞等: "脱毒马铃薯组培工厂化育苗成本核算", 《中国马铃薯》, vol. 24, no. 2, 31 December 2010 (2010-12-31), pages 120 - 124 * |
| 李春明第: "山杨工厂化育苗技术体系的研究", 《黑龙江生态工程职业学院学报》, vol. 24, no. 3, 31 May 2011 (2011-05-31), pages 19 - 21 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102936579A (en) * | 2012-09-23 | 2013-02-20 | 四川省农业科学院园艺研究所 | Liquid culture medium for promoting fruit tree tissue culture seedling growth |
| CN105638467A (en) * | 2016-01-06 | 2016-06-08 | 南平市华科生物科技有限公司 | Method for cultivating anoectochilus formosanus by using culture bag |
| CN109076959A (en) * | 2018-08-31 | 2018-12-25 | 湖州德清玖沐农业科技有限公司 | A kind of generic plant tissue culture culture medium and preparation method |
| CN112167064A (en) * | 2020-11-11 | 2021-01-05 | 山东农业大学 | Tissue culture and rapid propagation method for salvia miltiorrhiza |
| CN112772412A (en) * | 2020-12-30 | 2021-05-11 | 吴有光 | Method for performing open type plant tissue culture and rapid propagation by utilizing self-standing self-sealing film bag |
| CN116458426A (en) * | 2023-03-20 | 2023-07-21 | 云浮市南药研究院 | Energy-saving simple plant tissue culture aseptic operation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102349445B (en) | 2013-02-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102550411B (en) | Method for producing pre-basic seeds of potatoes | |
| CN102349445B (en) | Method for producing tissue culture seedlings on assembly line with low cost | |
| Prakash et al. | Culture media and containers | |
| JP2006254832A (en) | Solid medium | |
| JP5612605B2 (en) | Clone seedling production method | |
| CN107439376B (en) | A kind of in vitro culture joint culture medium and the in-vitro culture method of rhodiola | |
| CN102318559A (en) | Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method | |
| CN100374011C (en) | Method for tissue culture of lily flowers | |
| CN101707980B (en) | Sugarcane tissue culture seedling bagging outdoor rooting culture method | |
| CN104365485B (en) | A kind of smoothbark birch tissue cultured seedling is taken root the method for substrate and rooting and transplant thereof | |
| JP2009118835A (en) | Liquid culture method in plant seedling production which raises seedling from runner | |
| CN109220802B (en) | Tissue culture and rapid propagation method for corydalis amabilis | |
| CN105638455B (en) | A kind of method and culture medium that Drying pepper haplobiont is obtained by Anther Culture | |
| CN102668990A (en) | Plant tissue culturing method and device | |
| CN104885945B (en) | A kind of Fructus Musae chemical disinfection tissue culture method | |
| CN106550875B (en) | For the MS culture mediums of rouge radish tissue cultures, the quick breeding method for tissue culture of adventitious shoots culture base and rouge radish | |
| CN102823504B (en) | Eucalypt tissue culture medium | |
| CN103416300B (en) | Utilize the method for tuber of pinellia stem rapid breeding | |
| CN107135948A (en) | A kind of method that Camellia nitidissima spray cultivates tissue-cultured seedling under sunlight conditions | |
| CN106135007A (en) | A kind of sterilizing being suitable to trace Orchid Seeds during in vitro conservation and inoculation method | |
| Jiménez et al. | Micropropagation of bamboo species through axillary shoot proliferation | |
| Chandra et al. | Synthetic seed—Future prospects in crop improvement | |
| CN105379619A (en) | Preparation method for test-tube micro-bulbodium of Pinellia ternana | |
| CN102067818A (en) | Inducing technology of test tube lotus root | |
| CN103907531A (en) | Plant tissue culture production process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130206 Termination date: 20160811 |