CN1969612B - Novel method for producing culture medium by putting them into tissue-culturing bags inside a vessel - Google Patents
Novel method for producing culture medium by putting them into tissue-culturing bags inside a vessel Download PDFInfo
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- CN1969612B CN1969612B CN2006101241918A CN200610124191A CN1969612B CN 1969612 B CN1969612 B CN 1969612B CN 2006101241918 A CN2006101241918 A CN 2006101241918A CN 200610124191 A CN200610124191 A CN 200610124191A CN 1969612 B CN1969612 B CN 1969612B
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- substratum
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- metal vessel
- culture medium
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Abstract
The invention discloses a culturing method of bagged culture medium through loading container, which comprises the following steps: loading culture medium into plastic film bag; forming a group every 5-10 bags clamped by one ferric chuck; putting multiple ferric chucks into a metal container with breathing holes uniformly distributed on its wall to sterilize at high temperature, the metal container has a rectangular parallelepiped shape and includes a container body and a container cap, on the every sides of the metal container there is distributed 5-10 breathing holes, the ferric chuck clamps the opening of the bagged culture medium and is suspended on the ferric wire in the said container; after sterilization, picking out the bagged culture medium from the aseptic culturing room and then inoculating, sealing the opening of the bag which has already loaded culture medium. The objective of the invention is to provide a culturing method of bagged culture medium, wherein the metal container can be repeatedly used, the operation is simple, and sterilization is thorough, output of the culture medium at one time is high and the yield of the culture medium is high.
Description
[technical field]
The present invention relates to a kind of method of tissue culture, furtherly be a kind of method that container is cultivated packed substratum of contain carrying that adopts.
[background technology]
The plant tissue culture technique engineering has been the quick production technology of widely used seedling in the modern agriculture production technology, China has many units carrying out each kind of plant, flowers commercial production in batches, has obtained remarkable economic efficiency at banana seedling production field especially.At present, the conventional container that tissue culture technique adopted mainly is wide-necked bottle, triangular flask, Cans, the bottle that falls into water, and the external application plastics bag is also arranged.The shortcoming of external application vial is: 1 a large amount of culturing bottle takes up room greatly, and weight is big, disposable having high input, cost height; The expense of 2 cleanings, autoclaving and illumination cultivation is big, and efficient is low; 3 transportation inconvenience, expense is big.
The invention of Chinese patent 95114206.2 has demonstrated fully modern tissue culture new developing direction, but how to solve the packed substratum high-temperature sterilization of plastics, the increase capacity, reduce the method for polluting, up to the present also be to use kraft paper to wrap up packed substratum sterilization, its shortcoming is that kraft paper wraps up packed substratum comparatively small amt, substratum substratum under High Temperature High Pressure can overflow sack in the bag, cause the seedling pollution rate higher, and kraft paper wraps up packed substratum and cannot be repeatedly used, the production cost height.
[summary of the invention]
The invention provides reusable Sheng and carry the metal vessel of substratum, simple to operate, sterilization thoroughly, the disposable productive rate height of substratum, a kind of method that container is cultivated packed substratum of carrying of containing that adopts that substratum output is big.
In order to solve the problem of above-mentioned existence, the present invention has adopted following technical proposal:
A kind of employing involved in the present invention contained the method that year container is cultivated packed substratum, and it comprises the steps:
A: in the Plastic film Bag of earlier substratum being packed into;
B: is one group with per 5～10 bags of packed substratum with an agrafe folder;
C: will organize the agrafe that accompanies packed substratum more and be encased in and be evenly equipped with in the metal vessel of breather hole on facing the wall and meditating;
D: the metal vessel of each being filled packed substratum is tight its parcel with pachydermia paper or cloth;
E: the good metal vessel of parcel is put into 119～125 ℃ and 1.1 atmospheric sterilization nests carries out high-temperature sterilization, sterilized 20～30 minutes;
F: the metal vessel that sterilization is finished is together with the whole sterile culture chamber of moving to of the packed substratum in it, inoculates after taking out packed substratum, and the sack that inserts culture is sealed;
Aforesaid a kind of method that container is cultivated packed substratum of carrying of containing that adopts, it is characterized in that the rectangular parallelepiped that described metal vessel is made up of container body and container cover, be evenly equipped with the breather hole of 5～10 ventilation usefulness on each face of metal vessel, described breather hole is symmetrically distributed on six faces of described metal vessel in twos;
Aforesaid a kind of method that container is cultivated packed substratum of carrying of containing that adopts, the sack that it is characterized in that described packed substratum up, agrafe is clamped packed substratum sack and is suspended on the intravital iron wire of described container, and described iron wire two ends are separately positioned on container body two opposite faces in the paired breather hole;
Aforesaid a kind of the employing contained the method that year container is cultivated packed substratum, it is characterized in that hanging with on every described iron wire 3～7 agrafes, and the packed substratum proper alignment that hangs on each iron wire also is full of the space in the whole metal vessel;
Aforesaid a kind of the employing contained the method that year container is cultivated packed substratum, it is characterized in that described metal vessel wall thickness is 0.3～1.0cm, and metal vessel can adopt stainless steel substrates, galvanized sheet iron or aluminium flake to make.
The invention has the beneficial effects as follows:
1, metal vessel is reusable, it is big to contain year packed substratum quantity, disposable productive rate height, with low cost, operate simple and easyly, guaranteed that also packed substratum reaches the sterilisation effect of aseptic standard, reduce substratum and overflow contaminated problem, more can in identical disinfection space, place maximum substratum, be lowered into greatly and produce cost and energy consumption, be convenient to tissue culture medium (TCM) and carry out scale operation.
2, metal vessel is in high-temperature sterilization, and packed substratum freely is suspended on the iron wire, puts neatly, and packed substratum sack significantly reduces overflowing of packed substratum up when putting, thereby has reduced pollution, has significantly improved productive rate after the substratum high-temperature sterilization.
3, the breather hole of uniform some amount on each face of metal vessel was sterilized 20～30 minutes under 119～125 ℃ and 1 atmospheric condition when sterilization nest inner control temperature, can receive the effect of thorough sterilization, and sterilisation quality improves greatly.
[description of drawings]
The present invention is described in further detail below in conjunction with accompanying drawing.
Fig. 1 is a user mode stereographic map of the present invention;
The structural representation that Fig. 2 disassembles for invention.
[embodiment]
As shown in the figure, the present invention relates to a kind of the employing and contain the method that year container is cultivated packed substratum, it comprises the steps:
Elder generation packs the culture block that obtains after plant nutrition formula solution and the resin heating for dissolving in the Plastic film Bag; Be one group with per 5～10 bags of packed substratum with an agrafe folder again; To organize the agrafe that accompanies packed substratum more then and be encased in and be evenly equipped with in the metal vessel 3 of breather hole 4 on facing the wall and meditating, and allow packed substratum fill spaces in the whole metal vessel 3; Metal vessel 3 usefulness pachydermia paper or the cloth of at last each being filled packed substratum are tight its parcel; The good metal vessel 3 of parcel is put in 119～125 ℃ and 1.1 atmospheric cultivation nests carries out high-temperature sterilization, sterilized 20～30 minutes; The metal vessel 3 that sterilization is finished is together with the whole sterile culture chamber of moving to of the packed substratum in it, inoculates after taking out packed substratum, and the sack that inserts culture is sealed.
The rectangular parallelepiped that described metal vessel 3 is made up of container body 1 and container cover 2, be evenly equipped with the breather hole 4 of 5～10 ventilation usefulness on each face of metal vessel 3, described breather hole 4 is symmetrically distributed on six faces of described metal vessel in twos, during the packed substratum of tissue culture, high-temperature gas in the sterilization nest enters metal vessel 3 by breather hole 4, packed substratum in the metal vessel 3 is carried out disinfection, when sterilization nest inner control temperature was sterilized 20～30 minutes under 119～125 ℃ and 1 atmospheric condition, can receive the effect of thorough sterilization, sterilisation quality improves greatly; When loading before the sterilization, the sack of packed substratum up, agrafe is clamped packed substratum sack and is suspended on the iron wire 5 in the described container body 1, substratum is easy to overflow in bag in the time of can preventing high-temperature sterilization like this, described iron wire 5 two ends are separately positioned on 1 liang of opposite face of said vesse body in the paired breather hole 4, only need during use iron wire 5 is inserted breather hole 4 interior getting final product, very convenient.
Among the present invention, iron wire 5 adopts 14
#Number iron is made, and hangs with 3～7 agrafes on every described iron wire 5, and the packed substratum proper alignment that hangs on each iron wire 5 also is full of space in the whole metal vessel 3; The metal vessel of the packed substratum high-temperature sterilization of tissue culture has adopted the zinc coated sheet of wall thickness 0.3～0.5cm, large and small size design is also very reasonable, its volume is the 10x7x6.5cm rectangular parallelepiped, once can load the packed substratum about 150～200 bags, the packed substratum controllable number of loading is in proper range, guaranteed after metal vessel 3 is moved into the sterile culture chamber, can inoculate rapidly, prevent substratum to be exposed to airborne overlong time and influence inoculation quality that metal vessel 3 also can adopt stainless steel substrates or aluminium flake to make; Because the metal vessel of rectangular parallelepiped can make full use of the space of sterilized room, adopt 15 kilowatts sterilization nest and use the metal vessel 3 among the present invention to carry out disinfection, quantity that once can sterilized culture is equivalent to use the sterilization bag of substratum that the glass bottle of 400 250ml loads or about 1700～2000 bags or the substratum that kraft paper loads, and productivity effect is existing 3～5 times; When metal vessel 3 loads packed substratum, because packed substratum freely is suspended on the iron wire 5, put neatly, packed substratum sack significantly reduces overflowing of packed substratum up when putting, thereby has reduced pollution; And metal vessel 3 was both reusable in high-temperature sterilization, breather hole 4 set on it can reach good sterilising effect again, guarantee that also packed substratum can not overflow sack under High Temperature High Pressure, reduce the substratum pollution problems, more can in identical disinfection space, place maximum substratum, a large amount of energy consumptions have been saved, remarkable in economical benefits.
Claims (5)
1. one kind is adopted Sheng to carry the method that container is cultivated packed substratum, and it comprises the steps:
A: in the Plastic film Bag of earlier substratum being packed into;
B: is one group with per 5～10 bags of packed substratum with an agrafe folder;
C: will organize the agrafe that accompanies packed substratum more and be encased in and be evenly equipped with in the metal vessel (3) of breather hole (4) on facing the wall and meditating;
D: the metal vessel (3) of each being filled packed substratum is tight its parcel with pachydermia paper or cloth;
E: the good metal vessel (3) of parcel is put in 119～125 ℃ and 1.1 atmospheric sterilization nests carries out high-temperature sterilization, sterilized 20～30 minutes;
F: the metal vessel (3) that sterilization is finished is together with the whole sterile culture chamber of moving to of the packed substratum in it, inoculates after taking out packed substratum, and the sack that inserts culture is sealed.
2. a kind of method that container is cultivated packed substratum of carrying of containing that adopts according to claim 1, it is characterized in that the rectangular parallelepiped that described metal vessel (3) is made up of container body (1) and container cover (2), be evenly equipped with the breather hole (4) of 5～10 ventilation usefulness on each face of metal vessel (3), described breather hole (4) is symmetrically distributed on six faces of described metal vessel in twos.
3. a kind of method that container is cultivated packed substratum of carrying of containing that adopts according to claim 2, the sack that it is characterized in that described packed substratum up, agrafe is clamped packed substratum sack and is suspended on the iron wire (5) in the described container body (1), and described iron wire (5) two ends are separately positioned on container body (1) two opposite face in the paired breather hole (4).
4. a kind of method that container is cultivated packed substratum of carrying of containing that adopts according to claim 3, it is characterized in that hanging with on every described iron wire (5) 3～7 agrafes, each iron wire (5) is gone up the packed substratum proper alignment that hangs and is full of the interior space of whole metal vessel (3).
5. a kind of employing the according to claim 1 contained the method that year container is cultivated packed substratum, it is characterized in that described metal vessel (3) wall thickness is 0.3～1.0cm, and metal vessel (3) adopts stainless steel substrates, galvanized sheet iron or aluminium flake to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101241918A CN1969612B (en) | 2006-12-09 | 2006-12-09 | Novel method for producing culture medium by putting them into tissue-culturing bags inside a vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101241918A CN1969612B (en) | 2006-12-09 | 2006-12-09 | Novel method for producing culture medium by putting them into tissue-culturing bags inside a vessel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1969612A CN1969612A (en) | 2007-05-30 |
| CN1969612B true CN1969612B (en) | 2011-03-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101241918A Expired - Fee Related CN1969612B (en) | 2006-12-09 | 2006-12-09 | Novel method for producing culture medium by putting them into tissue-culturing bags inside a vessel |
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| CN (1) | CN1969612B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869075A (en) * | 2010-06-07 | 2010-10-27 | 广西壮族自治区甘蔗研究所 | Method for storing cane liquid culture medium bags in metal container |
| CN105638467B (en) * | 2016-01-06 | 2018-06-05 | 南平市华科生物科技有限公司 | A kind of method that roxburgh anoectochilus terminal bud is cultivated with culture bag |
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- 2006-12-09 CN CN2006101241918A patent/CN1969612B/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1969612A (en) | 2007-05-30 |
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