CN1593109A - Plant tissue culture method using disposable transparent polypropylene plastic cup - Google Patents
Plant tissue culture method using disposable transparent polypropylene plastic cup Download PDFInfo
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- CN1593109A CN1593109A CN 200410040149 CN200410040149A CN1593109A CN 1593109 A CN1593109 A CN 1593109A CN 200410040149 CN200410040149 CN 200410040149 CN 200410040149 A CN200410040149 A CN 200410040149A CN 1593109 A CN1593109 A CN 1593109A
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Abstract
The invention discloses a process for proceeding plant tissue culture through utilizing disposable transparent polypropylene plastic cups, which comprises, storing transparent plastic cups as tissue culture medium containers and culture medium formulated by the conventional technology into a high pressure sterilizing pan, sterilizing 20-30 minutes under the controlled temperature of 119-125 deg C, split charging the culture medium into transparent plastic cups under germ-free condition, inoculating with the conventional methods, sealing the cup mouths with covers and plastic paper, charging culture chamber for culturing.
Description
Technical field: the present invention relates to a kind of technology of utilizing Plant Tissue Breeding to produce seedling fast, particularly a kind of method of utilizing disposable transparent polypropylene plastics cup to carry out Plant Tissue Breeding.
Background technology: plant tissue culture technique has been the quick production technology of widely used seedling in the modern agriculture production technology, China has many units carrying out the commercial production in batches of each plant species, has obtained bigger economic benefit at agricultural production.The conventional at present container that tissue culture technique adopted mainly is a vial, and as triangular flask, Cans, liquid medicine bottle, also the someone utilizes plastic film bag.The shortcoming of utilizing vial is the transport difficulty height, and sterilization takies big quantity space, and energy consumption is big, and glass container takies substantial contribution, simultaneously, also will expend great amount of manpower and expense when cleaning.The shortcoming of utilizing plastic sack is an operating difficulties in the seeded process, and culture space is little, irregular simultaneously, and many plants are cultivated with plastic sack and are not suitable for.
Summary of the invention: the object of the present invention is to provide a kind of method of utilizing disposable transparent polypropylene plastics cup to carry out Plant Tissue Breeding, this method is with low cost, processing ease, can be fit to various Plant Tissue Breeding, the seedling production method of breeding fast, by adopting production cost low, lightweight expendable transparent plastic cup is as tissue culture vessel, to replace existing vial and plastic sack, reduced production cost effectively, more convenient operation simultaneously, can be extensive use of in more Plant Tissue Breeding field, overcome the deficiencies in the prior art preferably.
Formation of the present invention: place the medium of tissue culture vessel transparent plastic cup and technology preparation routinely in the high-pressure sterilizing pot respectively, the control temperature was sterilized under 119～125 ℃ condition 20～30 minutes, taking out the back installs to the medium branch in the transparent plastic cup under germ-free condition, inoculate with conventional method again, and with bowl cover or plastic paper rim of a cup is sealed, put into culturing room and cultivate.
It is 0.05mm-0.2mm that tissue culture vessel transparent plastic cup has adopted wall thickness, and volume is 150ml-1000ml, high temperature-resistant disinfected transparent polypropylene plastic cup.
The medium of cultivating packing in the cup can be the solid culture medium that is placed with agar solid gelling agent, can be the liquid nutrient medium that does not contain gelling agent also, and is that cup and medium separately after the sterilization, are carried out packing in superclean bench.
Compared with the prior art, the present invention is owing to adopted cost low, lightweight transparent plastic cup is as tissue culture vessel, to replace existing vial or plastic sack, the function and the advantage of prior art have not only been guaranteed, also have following characteristics simultaneously: 1, the space that occupies in sterilization of plastic cup is lacked than vial, the required space of vial of 100 250ml of sterilization can sterilize 350 cups and corresponding medium, and productivity effect is existing 3.5 times; 2, plastic cup is more neat than plastic sack as culture vessel, and is easy and simple to handle, is more suitable for the cultivation of various plants; 3, the seedling weight of plastic cup is lighter than vial seedling, is more conducive to remote transportation, has avoided the phenomenon of bag training seedling owing to crush injury seedling in the transportation again, and simultaneously, it is neat that plastic cup cup training seedling is trained seedling than bag; 4, remove the link of cleaning vial from, saved the storage area of vial; 5, plastic sack is because thin and seal problem, is punctured and seals bad easily, and the transparent plastic cup but can overcome this shortcoming preferably.
Adopt the transparent plastic cup to carry out Plant Tissue Breeding, have in light weight, convenient long-distance transport, because shape is neat, culture space is big than culture bag, therefore, more can be fit to various Plant Tissue Breeding, both possessed the advantage that the traditional glass bottle is cultivated seedling, overcome the high shortcoming of production cost, overcome plastic sack again owing to there is not solid shape to damage and inoculate the shortcoming of operating difficulties to cultivating plant easily, and possessed both lightly in transit, be not easy to be punctured and to seal the bad advantage that easily pollutes again.
Embodiment: the present invention has changed traditional tissue culture vessel, by utilizing conventional medium, select suitable temperature and illumination to carry out Plant Tissue Breeding according to different plants, for example: the wall thickness 0.1mm that selection is very easily purchased on the market, volume be the transparent plastic cup of 300ml as culture vessel, medium packing and inoculation are carried out in container and the conventional medium back of sterilizing respectively in superclean bench.
Embodiment: adopt wall thickness 0.1mm, volume is that the transparent plastic cup of 300ml is a tissue culture vessel, with container and medium respectively in high-pressure sterilizing pot, sterilization is 20 minutes under 120 ℃ condition, and with the medium packing, each plastic cup is inoculated after putting into the 30-50ml medium according to a conventional method in superclean bench in the taking-up back, then with the plastics bowl cover or the long 20cm that disinfect, wide 20cm plastic paper with the rim of a cup sealing, is put into culturing room with the plastic cup of good seal and is cultivated.Culture medium prescription as potato test-tube plantlet is: MS+2mg/LNAA+3% sucrose, PH5.6.The condition of culture of culturing room is: temperature 20-25 ℃, and illumination every day 16 hours, intensity of illumination is 2000LOX, cultivates promptly switchable after 20 days.
Claims (3)
1. method of utilizing disposable transparent polypropylene plastics cup to carry out Plant Tissue Breeding, it is characterized in that: place the medium of tissue culture vessel transparent plastic cup and technology preparation routinely in the high-pressure sterilizing pot respectively, the control temperature was sterilized under 119～125 ℃ condition 20～30 minutes, taking out the back installs to the medium branch in the transparent plastic cup under germ-free condition, inoculate with conventional method again, and with bowl cover or plastic paper rim of a cup is sealed, put into culturing room and cultivate.
2. the method for utilizing disposable transparent polypropylene plastics cup to carry out Plant Tissue Breeding according to claim 1, it is characterized in that: it is 0.05mm-0.2mm that tissue culture vessel transparent plastic cup has adopted wall thickness, volume is 150ml-1000ml, high temperature-resistant disinfected transparent polypropylene plastic cup.
3. the method for utilizing disposable transparent polypropylene plastics cup to carry out Plant Tissue Breeding according to claim 1, it is characterized in that: the medium of cultivating packing in the cup can be the solid culture medium that is placed with agar solid gelling agent, it also can be the liquid nutrient medium that does not contain gelling agent, and be that cup and medium separately after the sterilization, are carried out packing in superclean bench.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410040149 CN1593109A (en) | 2004-07-05 | 2004-07-05 | Plant tissue culture method using disposable transparent polypropylene plastic cup |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410040149 CN1593109A (en) | 2004-07-05 | 2004-07-05 | Plant tissue culture method using disposable transparent polypropylene plastic cup |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1593109A true CN1593109A (en) | 2005-03-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410040149 Pending CN1593109A (en) | 2004-07-05 | 2004-07-05 | Plant tissue culture method using disposable transparent polypropylene plastic cup |
Country Status (1)
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| CN (1) | CN1593109A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103907531A (en) * | 2013-01-06 | 2014-07-09 | 张征 | Plant tissue culture production process |
| CN110667908A (en) * | 2019-10-21 | 2020-01-10 | 北部湾大学 | Tissue culture seedling packaging method suitable for long-distance transportation |
-
2004
- 2004-07-05 CN CN 200410040149 patent/CN1593109A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103907531A (en) * | 2013-01-06 | 2014-07-09 | 张征 | Plant tissue culture production process |
| CN110667908A (en) * | 2019-10-21 | 2020-01-10 | 北部湾大学 | Tissue culture seedling packaging method suitable for long-distance transportation |
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