CN103184140B - Photosynthetic microbial culture apparatus and mounting method thereof - Google Patents
Photosynthetic microbial culture apparatus and mounting method thereof Download PDFInfo
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- CN103184140B CN103184140B CN201110446616.8A CN201110446616A CN103184140B CN 103184140 B CN103184140 B CN 103184140B CN 201110446616 A CN201110446616 A CN 201110446616A CN 103184140 B CN103184140 B CN 103184140B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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Abstract
The present invention relates to a culture apparatus and a mounting method thereof. The culture apparatus includes a main body composed of at least two brackets. The main body is provided with a wire net for connecting the brackets. The bracket is of a triangle structure with at least one movable opening side and is composed of a bottom beam and at least a first side beam and a second side beam. The main body is inside provided with a transparent culture vessel fitted with the inner sides of the at least two brackets. The culture apparatus of the invention is low in center of gravity, good in structural stability, low in requirement for flatness of the ground, and suitable for outdoor large-scale farming. According to the present invention, the triangle structure capable of movably opening and closing is used. The wire net at one side can be opened to facilitate the assembling and unassembling of plastic bags. Further, the culture apparatus is simple in structure and good in airtight, and is suitable for outdoor industrial scale breeding. Moreover, the raw materials of the culture apparatus can be processed into preformed units in the factory, on-site installation workload is small, and operation is easy.
Description
Technical field
The present invention relates to a kind of bio-reactor, particularly relate to a kind of photosynthetic microorganism culture apparatus and installation method thereof.
Background technology
Photosynthetic microorganism or vegetable cell, can utilize the form inorganicss such as sun power, water and simple mineral substance synthesis of organic substance and hydrogen, methane.Micro-algae is exactly one of them Typical Representative, and it is the individual small photosynthetic autotrophs unicellular organism of a class, has the features such as widely distributed, of a great variety, photosynthetic efficiency is high, fast growth, strong adaptability.The annual fixing CO of micro-algae
2account for 40% of global net photosynthesis output, add it and be rich in the high value natural pigments such as ester class, hydro carbons, albumen, soluble polysaccharide, astaxanthin, β-carotene, therefore the today attracting tremendous attention in problems such as environmental protection, the energy and health, micro-algae more and more receives people's concern.
Extensive, the low-cost cultivation of micro-algae are the bottlenecks of the micro-algae industrialized development of restriction, and exploitation simple and easy, high-efficient culture device is the key of micro-algae large-scale cultivation.Successively having there is pillar, tubular type, the type such as board-like in the research and development of culture apparatus, in recent years due to the advantage of board-like photosynthetic microorganism culture apparatus at aspects such as photosynthetic efficiencies, has become industry focal point.Board-like photosynthetic microorganism culture apparatus is gained the name because its outward appearance is tabular (rectangular-shaped), mainly comprises mechanically resistant material formula and the large class of soft material formula two.The board-like photosynthetic microorganism culture apparatus of mechanically resistant material consists of transparent stiff board material such as sheet glass or PVC plates, sometimes also can be aided with skeleton construction; The board-like photosynthetic microorganism culture apparatus of soft material consists of wire frame and plastics bag, more simple with the board-like photosynthetic microorganism culture apparatus structure compared of mechanically resistant material, cost is more cheap, be easier to industrialization amplifies.But it still exists structural stability deficiency, plastics bag is changed the problems such as difficulty.
United States Patent (USP) WO2004/074423A2, WO2005/006838 and Chinese patent 200720023436.8 disclose the board-like photosynthetic microorganism culture apparatus consisting of silk screen and plastics bag.These board-like photosynthetic microorganism culture apparatuses consist of silk screen, skeleton construction and plastics bag.Silk screen and skeleton construction form tabular cavity, and intensity is provided, and keep the profile of culture apparatus; Plastics bag is lining in silk screen inside cavity, and culture is housed.By pneumatic tube, to input air and carbonic acid gas in culture apparatus, realize that carbon source is supplied with and the circulation stirring of nutrient solution, to carry out the cultivation of culture.
Mainly there is following technological deficiency in above-mentioned board-like photosynthetic microorganism culture apparatus: 1, the stability of structure is not enough, because its radial section is the narrower rectangle in base, therefore center of gravity is higher, thereby the planeness on ground is had relatively high expectations; 2, the handiness of device is not high, makes the dismounting difficulty of plastics bag; Due in the breeding process of culture for guaranteeing culture quality, need periodic replacement plastics bag, to narrow and grow in the silk screen cavity of (during mass-producing application, culture apparatus may reach tens to up to a hundred meters) and spreads into plastics bag, actually operating is very difficult.
Summary of the invention
The object of the invention is to propose a kind of simple in structure, be convenient to installing/dismounting, lower, the constitutionally stable trilateral photosynthetic microorganism of center of gravity culture apparatus, thereby increase operability and the environmental compatibility of culture apparatus.
For achieving the above object, the invention provides a kind of culture apparatus, described culture apparatus comprises the body consisting of at least two pack supports, on described body, be also provided with the silk screen that connects described each pack support, the triangular structure that described support is the movable switching at least on one side that consists of bottom girder and at least the first curb girder and the second curb girder, the transparent culture vessel that described body interior is provided with and match in described at least two pack supports inner sides.
Preferably, described the first curb girder is with described bottom girder for being fixedly connected with, and described the second curb girder and described bottom girder be for being rotationally connected, between described the second curb girder and described the first curb girder for being flexibly connected.
More preferably, described transparent culture vessel is tubular plastics bag, and described plastics bag top is provided with hot pressing broadside, and described hot pressing broadside is provided with at least one suppending hole.
Further, described at least two pack supports are arranged in array.
Preferably, described plastics bag is provided with outer catheter and/or detects mouth.
More preferably, the bottom of described plastics bag is provided with parallel gas feed mechanism.
Preferably, described culture apparatus also comprises the mixing pit being connected with described transparent culture vessel with pump by pipeline.
Preferably, described culture apparatus comprises two groups or more body, is at least provided with a mixing pit between two adjacent groups body.
Preferably, described silk screen comprise at least two pack support the first curb girders described in connection the first side silk screen, connect described in the second side silk screen of at least two pack support the second curb girders, and the bottom surface silk screen that connects at least two pack support bottom girders.
More preferably, the bottom mesh-density of described the first side silk screen and the second side silk screen is greater than upper grid density.
Preferably, at described bottom girder, be flexibly connected the outside of end with the second curb girder, be also provided with locating part.
Another object of the present invention is to provide a kind of installation method of culture apparatus, this installation method comprises the steps: to manufacture auxiliary mould, on mechanically resistant material, according to the distance of two adjacent groups support, offers two grooves that match with curb girder and/or bottom girder width dimensions; Assembling support, the first curb girder and bottom girder installation combination is fixing, and will between the second curb girder and bottom girder, be rotationally connected; Assembling body, is used auxiliary mould, and the first curb girder that at least two groups are fixed as one and bottom girder form array; On each bottom girder, lay after the silk screen of bottom surface, the first side screw net bottom end high spot is inserted in the gap of silk screen long edge position, bottom surface, and use auxiliary mould by temporary fixed between the first side silk screen and the first curb girder; Transparent culture vessel is installed, transparent culture vessel is suspended on to the prominence at the first silk screen top, side; After in the gap of the second side screw net bottom end high spot insertion silk screen long edge position, bottom surface, the second curb girder is arranged on to the periphery of the second side silk screen, and buckles the active connection on the first curb girder and the second curb girder top.
Preferably, described method is also included in described bottom girder and is flexibly connected the step that the outer upper of holding arranges locating part with the second curb girder.
Based on technique scheme, advantage of the present invention is:
Culture apparatus center of gravity of the present invention is lower, and structural stability is better, less demanding to the planeness on ground, is more suitable for outdoor large-scale cultivation; The present invention has adopted the triangular structure of movable switching, and at least one side silk screen can be opened, and is convenient to handling and the replacing of plastics bag; In addition, the present invention is simple in structure, stopping property is better, is a kind of closed culture apparatus of cheapness that is suitable for outdoor industrially scalable cultivation; And starting material of the present invention can become prefabricated component in factory process in advance, on-site installation work amount is less, easy and simple to handle.
Accompanying drawing explanation
Accompanying drawing described herein is used to provide a further understanding of the present invention, forms the application's a part, and schematic description and description of the present invention is used for explaining the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 is the structural representation of a kind of preferred embodiment of culture apparatus body of the present invention;
Fig. 2 is the side-looking structural representation of a kind of embodiment of culture vessel of the present invention;
Fig. 3 is the elevation cross-sectional view of structure shown in Fig. 2;
Fig. 4 is the schematic diagram of the initial installment state of installation method of the present invention;
Fig. 5 is the enlarged diagram of installation method auxiliary mould of the present invention;
Fig. 6 is the schematic diagram of installment state in the middle of installation method of the present invention;
Fig. 7 is the structural representation of the another kind of preferred embodiment of culture apparatus body of the present invention;
Fig. 8 is the side-looking structural representation of the another kind of embodiment of culture vessel of the present invention;
Fig. 9 is the elevation cross-sectional view of structure shown in Fig. 8;
Figure 10, Figure 11 are the structural representation of active connection of the present invention;
Figure 12 is the structural representation of another preferred embodiment of culture apparatus body of the present invention;
Figure 13 is the structural representation of another embodiment of culture apparatus of the present invention.
Embodiment
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Embodiment 1
As shown in Figure 1, culture apparatus of the present invention is mainly comprised of body and transparent culture vessel two portions, and wherein body is formed jointly by least two pack supports, and preferably, at least two described pack supports are arranged in array, and for example parallel rows is in a row, forms body part; On described body, be also provided with the silk screen that connects described each pack support.The triangular structure that support is the movable switching at least on one side that consists of bottom girder 5 and at least the first curb girder 1 and the second curb girder 2.Preferably, the first curb girder 1 is connected or is directly welded by fixed connecting piece 8 at place, base angle with bottom girder 5, and the second curb girder 2 is connected by rotating connector 9 at place, base angle with bottom girder 5, and the second curb girder 2 is connected with bottom girder 5, but can rotate around rotating connector 9.The first curb girder 1 is connected by active connection 7 at drift angle place with the second curb girder 2, and the first curb girder 1 can be opened as required at any time with being connected of the second curb girder 2.So, the first curb girder 1, the second curb girder 2 and bottom girder 5 have just formed stable triangular supports structure.A plurality of such triangular supports are arranged in array, and have just formed body construction of the present invention.Bottom surface silk screen 6 is laid in each bottom girder 5 places, and the first side silk screen 3 is positioned at the first curb girder 1 inner side, and the second side silk screen 4 is positioned at the second curb girder 2 inner sides.
As shown in Figures 2 and 3, culture vessel of the present invention is made by transparent material, is preferably tubular plastics bag 11.In the present embodiment, plastics bag 11 is oval tubular, and the top of plastics bag 11 is provided with hot pressing broadside 10 along its length, so that operations such as installation, suspensions.On hot pressing broadside 10, along plastics bag 11 length directions, be equipped with at a certain distance a suppending hole 14, when installing and changing plastics bag, can by suppending hole 14, plastics bag 11 integral body be hung over to the prominence on the first side silk screen 3 or the second side silk screen 4 tops, so that subsequent operationss such as cultivation, handling.Hot pressing broadside 10 places also leave several and make the inner outer catheter 12 communicating with external environment of plastics bag 11 and/or detection mouth 13 simultaneously.In the present embodiment, on plastics bag 11, be both provided with outer catheter 12, and be also provided with and detect mouth 13.Outer catheter 12, for the discharge of gas in plastics bag and foam solution, detects mouth 13 for measuring the insertion with instrument, probe and water supply, air-supply duct.Further preferably, water port 15 is also equipped with in the bottom of plastics bag 11, and after culturing process completes, nutrient solution can be discharged plastics bag 11 through water port 15.
Further, the plastics bag 11 of the present embodiment can be directly by commercially available transparent plastic material (note: the plastic cloth that now sell in market is to be all long tubular, and roll up along its length) the rear directly use of sealing, and open along its length several holes in its position near triangular supports drift angle, for liquid, the use of the insertion of input, output and the detector probe of gas.The girth of plastics bag cross section is less than or equal to the internal circumference of the triangular supports that silk screen surrounds.And in use procedure, the nutrient solution in plastics bag 11 is not exclusively filled, liquid level keeps certain distance apart from plastics bag top, in case liquid overflows from the hole at top when ventilating in plastics bag.This kind of technical scheme can greatly be dwindled manufacturing cost of the present invention, and reaches cultivation object.
The technical scheme of the present embodiment can solve that in prior art, culture apparatus center of gravity is too high, stability is not enough effectively, and then the technical problem that ground flat degree is had relatively high expectations; Also can make up prior art mostly be fixedly connected with, plastics bag handling difficulty, and in culturing process, occur as dirts such as foams, be difficult to the problems such as discharge.
Described in embodiment 1, the installation method of device comprises the steps:
As shown in Figure 4, Figure 5 and Figure 6, in the present embodiment, the first curb girder 1, the second curb girder 2, bottom girder 5 are made by galvanized steels wide 5 centimetres, thick 3 centimetres.The first 1.4 meters of curb girder 1 length, 48 centimetres of bottom girder 5 length, the first curb girder 1 is connected by fixed connecting piece 8 at base angle with bottom girder 5, and preferably, in the present embodiment, the first curb girder 1 is welded in fixed connection place, base angle with bottom girder 5.Auxiliary mould 16 by wood, porous plastics or bakelite etc. have some strength, be convenient to cutting or plastic mechanically resistant material make.Two grooves 17 of mould are stuck on two adjacent curb girders (as shown in Figure 4 and Figure 6), in the present embodiment, and apart 0.5 meter of adjacent curb girder, then bottom surface silk screen 6 is laid on all bottom girders 5.The second 1.4 meters of curb girder 2 length are bolted with bottom girder 5, and bolt is also not exclusively tightened, and makes to have 1 millimeter space between bottom girder 5 and the second curb girder 2, and the second curb girder 2 can be rotated round bolt.Certainly, between bottom girder 5 and the second curb girder 2, also can adopt other active connections or mode of connection.
The first side silk screen 3 and the second side silk screen 4 are high 1.3 meters, by 5 millimeters of thick linings, moulding silk screen forms, the bottom mesh-density of described the first side silk screen 3 and the second side silk screen 4 is greater than upper grid density, in the present embodiment, preferably 0.5 meter of height of silk screen is the grid of 5 centimeter square below, 0.5 meter of height is the rectangle grid of 10 centimetres wide 5 centimetres of length above, this kind of structure makes the first side silk screen 3 and the second side silk screen 4 fill with after nutrient solution at plastics bag 11, can provide enough support strengths for body and plastics bag 11.
Further, the bottom high spot of the first side silk screen 3 is inserted in the narrow slit of silk screen 6 long edge positions, bottom surface, then with mould, itself and the first curb girder 1 is temporary fixed.Plastics bag 11 is made by 1 mm thick transparent plastic film, is oval tubular, and opening end seals through heat seal.The hot pressing broadside 10 that extrudes 4 cm widths through hot pressing is gone up at top along its length.It is the suppending hole 14 of 1 centimetre that top flanging place is equipped with diameter along plastics bag length direction every 0.5 meter.Top flanging place simultaneously also leaves 1 every 1.5 meters of and makes the inner outer catheter 12 communicating with external environment of plastics bag, outer catheter 12 forms for the tubule (0.6 centimetre of diameter) made by mechanically resistant material or by the extension (0.6 centimetre of diameter) of plastics, every 2 meters of, also leaves and detects mouthfuls 13.Plastics bag 11 bottoms are processed water port 15 and water discharge valve 22 are housed through heat seal thermoplastic.The suppending hole of plastics bag 11 14 is enclosed within to the prominence at the first side silk screen 3 tops, again the bottom high spot of the second side silk screen 4 is inserted in the narrow slit of silk screen 6 long edge positions, bottom surface, finally the second curb girder 2 is wrapped in to the second side silk screen 4 peripheries, and buckle the active connection 7 on the first curb girder 1 and the second curb girder 2 tops.As shown in Figure 10 and Figure 11, active connection 7 described in the present embodiment comprises first part 29 and the second section 30 of the buckle that cooperatively interacts, this buckle locking structure, manufacture simple, easy to operate, can to make support body of the present invention form Stability Analysis of Structures, can open as required at any time by active connection 7 triangular structure.
Finally, as shown in Figure 7, at each bottom girder 5, be flexibly connected the top, outside (for the inner side of culture vessel is housed) of end with the second curb girder 2, a locating part is set respectively.Locating part described in the present embodiment is that 10 centimetres of length, inclination angle are the angle steel 19 of 60 degree, thereby can limit the scope of activity of the second curb girder 2, as shown in Figure 7.
In addition, as shown in Figure 8 and Figure 9, for reaching good carbon source, supply with and circulation stirring effect, in the bottom of plastics bag 11, parallel gas feed mechanism 20 is also set, this parallel gas feed mechanism 20 carries out air feed by airway 21 in from outside to plastics bag 11, thereby meets better fostering requirement.
Embodiment 2
Referring to Figure 12, in the present embodiment, the first 1.5 meters of curb girder 1 length, the second curb girder 2 1.8 meters of length, bottom girder 5 are grown 1 meter, three beam form right angle trilaterals, this kind of body construction can meet the arrangement requirement near places such as board walls, and other structures are identical with above-described embodiment 1.
Embodiment 3
As shown in figure 13, in the present embodiment, in plastics bag 11, gas supply mechanism is not housed, and described culture apparatus also comprises and the described mixing pit 27 or 28 being connected with described transparent culture vessel with pump 29 by pipeline, referring to Figure 13.Described culture apparatus comprises two groups or more body (as shown in two bodies 26 in Figure 13) and transparent culture vessel, between the transparent culture vessel of two adjacent groups, by mixing pit 27 or 28, is connected.The shape of mixing pit both can be high and narrow, shown in mixing pit 27 as shown in Figure 13; Also can be short and wide, shown in mixing pit 28 as shown in Figure 13, its shape is restriction not, material printing opacity or light tightly all can.Carbonic acid gas is supplied with, nutritive salt supplements, and results process all can carry out in mixing pit, and the transparent culture vessel in culture apparatus body is communicated with mixing pit, and nutrient solution flows into mixing pit voluntarily.In mixing pit, nutrient solution fully contacts with carbonic acid gas or nutrition, discharges the oxygen in solution simultaneously, then through impeller pump 29, the nutrient solution in mixing pit is injected to the intrinsic transparent culture vessel of another group.Between the transparent culture vessel of two adjacent groups, being at least provided with a mixing pit is connected respectively with both.
And, the present invention both can two groups bodies (containing transparent culture vessel) as shown in figure 13, form loop line; Also can pass through at least two group bodies (containing transparent culture vessel) and be connected with mixing pit in the mode of series, parallel and series-parallel connection, realize large-scale cultivation.
It should be noted that, the making material of body of the present invention and transparent culture vessel is not limited to the material described in embodiment, and any material that can meet culture apparatus desirable strength, toughness and corrosion resistant requirement all can be realized the present invention.
In the breeding process of each embodiment; can in nutrient solution, add the solid particulate close with nutrient solution density; as plastic pellet; make it to coexist and flow in reactor with nutrient solution one; in this process, solid particulate can produce collision with plastics bag; there is certain cleaning action, can reduce to a certain extent frustule in the absorption of plastic bag surface.
Obviously, those of ordinary skill in the art, can, with a kind of culture apparatus of the present invention and installation method thereof, form various types of biological culture instruments.
Finally should be noted that: above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; Although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the field are to be understood that: still can modify or part technical characterictic is equal to replacement the specific embodiment of the present invention; And not departing from the spirit of technical solution of the present invention, it all should be encompassed in the middle of the technical scheme scope that the present invention asks for protection.
Claims (13)
1. a photosynthetic micro-algae organism culturing device, it is characterized in that: described culture apparatus comprises the body consisting of at least two pack supports, on described body, be also provided with the silk screen that connects described each pack support, described support is the triangular structure by the movable switching at least on one side of bottom girder (5) and at least the first curb girder (1) and the second curb girder (2) formation, the transparent culture vessel that described body interior is provided with and match in described at least two pack supports inner sides.
2. culture apparatus according to claim 1, it is characterized in that: described the first curb girder (1) with described bottom girder (5) for being fixedly connected with, described the second curb girder (2) and described bottom girder (5) be for being rotationally connected, between described the second curb girder (2) and described the first curb girder (1) for being flexibly connected.
3. culture apparatus according to claim 2, it is characterized in that: described transparent culture vessel is tubular plastics bag (11), described plastics bag (11) top is provided with hot pressing broadside (10), and described hot pressing broadside (10) is provided with at least one suppending hole (14).
4. according to the culture apparatus described in claim 1 or 2 or 3, it is characterized in that: described at least two pack supports are arranged in array.
5. culture apparatus according to claim 4, is characterized in that: described plastics bag (11) is provided with outer catheter (12) and/or detects mouthful (13).
6. culture apparatus according to claim 4, is characterized in that: the bottom of described plastics bag (11) is provided with parallel gas feed mechanism (20).
7. culture apparatus according to claim 4, is characterized in that: described culture apparatus also comprises the mixing pit being connected with described transparent culture vessel with pump (29) by pipeline.
8. culture apparatus according to claim 7, is characterized in that: described culture apparatus comprises that two groups or more inside is provided with the body of transparent culture vessel, is at least provided with a mixing pit between two adjacent groups body.
9. culture apparatus according to claim 1, it is characterized in that: described silk screen comprise at least two pack support the first curb girders (1) described in connection the first side silk screen (3), connect described in the second side silk screen (4) of at least two pack support the second curb girders (2), and the bottom surface silk screen (6) that connects at least two pack support bottom girders (5).
10. culture apparatus according to claim 9, is characterized in that: the bottom mesh-density of described the first side silk screen (3) and the second side silk screen (4) is greater than upper grid density.
11. culture apparatuses according to claim 1, is characterized in that: at described bottom girder (5), be flexibly connected the outside of end with the second curb girder (2), be also provided with locating part.
The installation method of 12. 1 kinds of photosynthetic micro-algae organism culturing devices, comprises the steps:
Manufacture auxiliary mould, on mechanically resistant material, according to the distance of two adjacent groups support, offer two grooves that match with curb girder and/or bottom girder width dimensions;
Assembling support, the first curb girder and bottom girder installation combination is fixing, and will between the second curb girder and bottom girder, be rotationally connected;
Assembling body, is used auxiliary mould, and the first curb girder that at least two groups are fixed as one and bottom girder form array; On each bottom girder, lay bottom surface silk screen, and after the first side screw net bottom end high spot being inserted in the gap of silk screen long edge position, bottom surface, use auxiliary mould by temporary fixed between the first side silk screen and the first curb girder;
Transparent culture vessel is installed, transparent culture vessel is suspended on to the prominence at the first silk screen top, side; After in the gap of the second side screw net bottom end high spot insertion silk screen long edge position, bottom surface, the second curb girder is arranged on to the periphery of the second side silk screen, and buckles the active connection on the first curb girder and the second curb girder top.
13. installation methods according to claim 12, is characterized in that: described method is also included in described bottom girder and is flexibly connected the step that the outer upper of holding arranges locating part with the second curb girder.
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| CN201110446616.8A CN103184140B (en) | 2011-12-28 | 2011-12-28 | Photosynthetic microbial culture apparatus and mounting method thereof |
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| CN201110446616.8A CN103184140B (en) | 2011-12-28 | 2011-12-28 | Photosynthetic microbial culture apparatus and mounting method thereof |
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| CN103184140B true CN103184140B (en) | 2014-09-17 |
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| CN109251839B (en) * | 2017-07-14 | 2021-08-06 | 中国石油化工股份有限公司 | Device and method for culturing photosynthetic microorganisms by using sunlight |
| CN109536383A (en) * | 2018-08-31 | 2019-03-29 | 雷云飞 | A kind of activity of microalgae cytotrophy repairs the production line of liquid |
| JP2024130438A (en) * | 2023-03-14 | 2024-09-30 | 株式会社ちとせ研究所 | Culture Equipment |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145409A (en) * | 1995-09-12 | 1997-03-19 | 广东省农业科学院果树研究所 | Tissue culture method using polypropylene film bag as tissue culture packing vessel |
| CN201062270Y (en) * | 2007-06-07 | 2008-05-21 | 邹宁 | Industrialized scale economic optical bioreactor |
| CN101709264A (en) * | 2009-06-19 | 2010-05-19 | 新奥科技发展有限公司 | Optical bioreactor |
-
2011
- 2011-12-28 CN CN201110446616.8A patent/CN103184140B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1145409A (en) * | 1995-09-12 | 1997-03-19 | 广东省农业科学院果树研究所 | Tissue culture method using polypropylene film bag as tissue culture packing vessel |
| CN201062270Y (en) * | 2007-06-07 | 2008-05-21 | 邹宁 | Industrialized scale economic optical bioreactor |
| CN101709264A (en) * | 2009-06-19 | 2010-05-19 | 新奥科技发展有限公司 | Optical bioreactor |
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