CN103168119B - 用于产生、确认和使用单克隆抗体的方法和系统 - Google Patents

用于产生、确认和使用单克隆抗体的方法和系统 Download PDF

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Publication number
CN103168119B
CN103168119B CN201180039055.8A CN201180039055A CN103168119B CN 103168119 B CN103168119 B CN 103168119B CN 201180039055 A CN201180039055 A CN 201180039055A CN 103168119 B CN103168119 B CN 103168119B
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antibody
antibodies
library
protein
antigens
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Chinese (zh)
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CN103168119A (zh
Inventor
杰夫·D·伯克
恒·朱
塞斯·布莱克肖
丹尼尔·J·艾兴格
伊格纳西奥·皮诺
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Cdi Lab Corp
Johns Hopkins University
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Cdi Lab Corp
Johns Hopkins University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201180039055.8A 2010-06-16 2011-06-16 用于产生、确认和使用单克隆抗体的方法和系统 Active CN103168119B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35532910P 2010-06-16 2010-06-16
US61/355,329 2010-06-16
PCT/US2011/040789 WO2011159959A2 (en) 2010-06-16 2011-06-16 Methods and systems for generating, validating and using monoclonal antibodies

Publications (2)

Publication Number Publication Date
CN103168119A CN103168119A (zh) 2013-06-19
CN103168119B true CN103168119B (zh) 2016-05-11

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CN201180039055.8A Active CN103168119B (zh) 2010-06-16 2011-06-16 用于产生、确认和使用单克隆抗体的方法和系统

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US (3) US20130288910A1 (enExample)
EP (1) EP2582862B1 (enExample)
JP (2) JP2013533865A (enExample)
CN (1) CN103168119B (enExample)
CA (1) CA2802868A1 (enExample)
WO (1) WO2011159959A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012103260A2 (en) * 2011-01-25 2012-08-02 Cdi Laboratories High-throughput methods to produce, validate and characterize mmabs
US20140307931A1 (en) * 2013-04-15 2014-10-16 Massachusetts Institute Of Technology Fully automated system and method for image segmentation and quality control of protein microarrays
JP6715766B2 (ja) * 2013-06-21 2020-07-01 ザ ジョンズ ホプキンス ユニバーシティ ヒト膜蛋白質の機能および相互作用をプロファイリングするためのビリオンディスプレイアレイ
US20190391147A1 (en) * 2016-04-07 2019-12-26 Rowan University Methods and kits for detecting myo/nog cells in human adipose tissue and methods of use thereof
CN109470852A (zh) * 2018-12-07 2019-03-15 华南农业大学 一种母乳多克隆抗体免疫亲和柱及其制备方法
WO2025101487A1 (en) * 2023-11-06 2025-05-15 Bruker Cellular Analysis, Inc. Affinity assay for microfluidic devices

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1786298A (zh) * 2005-10-28 2006-06-14 上海博昂抗体生物科技有限公司 抗细胞或组织蛋白质单克隆抗体库的制备方法

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DE4134297A1 (de) * 1991-10-17 1993-04-22 Behringwerke Ag Monoclonale antikoerper gegen mycoplasma pneumoniae, diese produzierende hybridome, verfahren zu deren herstellung sowie deren verwendung
JP2002502038A (ja) * 1998-01-29 2002-01-22 ミラー，サミュエル プロテオーム分析のための高密度アレイ及びその方法及び組成物
US6794132B2 (en) * 1999-10-02 2004-09-21 Biosite, Inc. Human antibodies
WO2004046330A2 (en) * 2002-11-15 2004-06-03 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
WO2006033972A2 (en) * 2004-09-15 2006-03-30 Protometrix, Inc. Protein arrays and methods of use thereof
EP1802981B8 (en) * 2004-10-23 2012-09-12 BioSystems International SAS Expression profiling platform technology
JP2008521412A (ja) * 2004-11-30 2008-06-26 ベリデックス・エルエルシー 肺癌予後判定手段
WO2006124644A2 (en) * 2005-05-12 2006-11-23 Board Of Regents, The University Of Texas System Protein and antibody profiling using small molecule microarrays
EP1921142A1 (en) * 2006-11-07 2008-05-14 Cytos Biotechnology AG Selection of human monoclonal antibodies by eukaryotic cell display
WO2009108170A2 (en) * 2007-11-16 2009-09-03 Invitrogen Corporation Compositions and methods for determining immune status
WO2010011337A1 (en) * 2008-07-25 2010-01-28 Theraclone Sciences Methods and compositions for discovery of target-specific antibodies using antibody repertoire array (ara)
BR112012006678A2 (pt) * 2009-09-25 2016-05-10 Origene Technologies Inc método para criar um arranjo proteico, arranjo proteico e métodos para determinar a especificidade da ligação de um anticorpo ou preparação de anticorpos, para determinar biomarcadores de doenças, para diagnosticar uma doença, para monitorar as vias de transdução de sinais e para determinar interações entre pequenas moléculas e proteínas

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1786298A (zh) * 2005-10-28 2006-06-14 上海博昂抗体生物科技有限公司 抗细胞或组织蛋白质单克隆抗体库的制备方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Analyzing antibody specificity with whole proteome microarrays;Michaud 等;《Nature Biotechnology》;20031109;第21卷(第12期);第1509-1512页 *

Also Published As

Publication number Publication date
EP2582862B1 (en) 2016-04-13
WO2011159959A2 (en) 2011-12-22
CA2802868A1 (en) 2011-12-22
US20220363712A1 (en) 2022-11-17
US20130288910A1 (en) 2013-10-31
JP2013533865A (ja) 2013-08-29
CN103168119A (zh) 2013-06-19
JP2016222725A (ja) 2016-12-28
EP2582862A4 (en) 2014-01-15
WO2011159959A3 (en) 2013-03-28
HK1182745A1 (zh) 2013-12-06
US20180030086A1 (en) 2018-02-01
EP2582862A2 (en) 2013-04-24

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