CA2802868A1 - Methods and systems for generating, validating and using monoclonal antibodies - Google Patents
Methods and systems for generating, validating and using monoclonal antibodies Download PDFInfo
- Publication number
- CA2802868A1 CA2802868A1 CA2802868A CA2802868A CA2802868A1 CA 2802868 A1 CA2802868 A1 CA 2802868A1 CA 2802868 A CA2802868 A CA 2802868A CA 2802868 A CA2802868 A CA 2802868A CA 2802868 A1 CA2802868 A1 CA 2802868A1
- Authority
- CA
- Canada
- Prior art keywords
- antibodies
- antibody
- target
- library
- antigens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims description 106
- 230000002621 immunoprecipitating effect Effects 0.000 claims abstract description 50
- 108090000623 proteins and genes Proteins 0.000 claims description 279
- 108091007433 antigens Proteins 0.000 claims description 266
- 102000036639 antigens Human genes 0.000 claims description 266
- 239000000427 antigen Substances 0.000 claims description 261
- 102000004169 proteins and genes Human genes 0.000 claims description 229
- 230000027455 binding Effects 0.000 claims description 217
- 210000004027 cell Anatomy 0.000 claims description 180
- 108010026552 Proteome Proteins 0.000 claims description 108
- 241000282414 Homo sapiens Species 0.000 claims description 105
- 239000000758 substrate Substances 0.000 claims description 46
- 108090000144 Human Proteins Proteins 0.000 claims description 44
- 102000003839 Human Proteins Human genes 0.000 claims description 44
- 238000012216 screening Methods 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 23
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 22
- 241001465754 Metazoa Species 0.000 claims description 21
- 239000012472 biological sample Substances 0.000 claims description 20
- 210000001519 tissue Anatomy 0.000 claims description 19
- 238000003365 immunocytochemistry Methods 0.000 claims description 18
- 230000003053 immunization Effects 0.000 claims description 15
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 12
- 210000004909 pre-ejaculatory fluid Anatomy 0.000 claims description 12
- 210000004369 blood Anatomy 0.000 claims description 11
- 239000008280 blood Substances 0.000 claims description 11
- 102000039446 nucleic acids Human genes 0.000 claims description 11
- 108020004707 nucleic acids Proteins 0.000 claims description 11
- 150000007523 nucleic acids Chemical class 0.000 claims description 11
- 239000006166 lysate Substances 0.000 claims description 9
- 210000002751 lymph Anatomy 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- 210000001179 synovial fluid Anatomy 0.000 claims description 8
- 210000004381 amniotic fluid Anatomy 0.000 claims description 7
- 210000002381 plasma Anatomy 0.000 claims description 7
- 210000003296 saliva Anatomy 0.000 claims description 7
- 210000002700 urine Anatomy 0.000 claims description 7
- 208000005228 Pericardial Effusion Diseases 0.000 claims description 6
- 206010036790 Productive cough Diseases 0.000 claims description 6
- 210000001742 aqueous humor Anatomy 0.000 claims description 6
- 210000003567 ascitic fluid Anatomy 0.000 claims description 6
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 6
- 210000000941 bile Anatomy 0.000 claims description 6
- 210000004952 blastocoel Anatomy 0.000 claims description 6
- 210000001185 bone marrow Anatomy 0.000 claims description 6
- 210000002939 cerumen Anatomy 0.000 claims description 6
- 210000003756 cervix mucus Anatomy 0.000 claims description 6
- 210000001268 chyle Anatomy 0.000 claims description 6
- 210000004913 chyme Anatomy 0.000 claims description 6
- 210000002726 cyst fluid Anatomy 0.000 claims description 6
- 210000003722 extracellular fluid Anatomy 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 6
- 210000004700 fetal blood Anatomy 0.000 claims description 6
- 210000004251 human milk Anatomy 0.000 claims description 6
- 235000020256 human milk Nutrition 0.000 claims description 6
- 210000004914 menses Anatomy 0.000 claims description 6
- 210000003097 mucus Anatomy 0.000 claims description 6
- 210000001819 pancreatic juice Anatomy 0.000 claims description 6
- 210000004912 pericardial fluid Anatomy 0.000 claims description 6
- 210000004910 pleural fluid Anatomy 0.000 claims description 6
- 210000004908 prostatic fluid Anatomy 0.000 claims description 6
- 210000004915 pus Anatomy 0.000 claims description 6
- 210000002374 sebum Anatomy 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 6
- 210000003802 sputum Anatomy 0.000 claims description 6
- 208000024794 sputum Diseases 0.000 claims description 6
- 210000004243 sweat Anatomy 0.000 claims description 6
- 210000001138 tear Anatomy 0.000 claims description 6
- 239000011148 porous material Substances 0.000 claims description 5
- 239000011343 solid material Substances 0.000 claims description 5
- 229910052717 sulfur Inorganic materials 0.000 claims description 5
- 230000002427 irreversible effect Effects 0.000 claims description 3
- 230000002441 reversible effect Effects 0.000 claims description 3
- 230000003100 immobilizing effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 17
- 230000037361 pathway Effects 0.000 description 69
- 238000002493 microarray Methods 0.000 description 61
- 210000004408 hybridoma Anatomy 0.000 description 36
- 238000004458 analytical method Methods 0.000 description 33
- 206010028980 Neoplasm Diseases 0.000 description 31
- 201000011510 cancer Diseases 0.000 description 30
- 230000014509 gene expression Effects 0.000 description 25
- 102000040945 Transcription factor Human genes 0.000 description 21
- 108091023040 Transcription factor Proteins 0.000 description 21
- 238000002487 chromatin immunoprecipitation Methods 0.000 description 20
- 238000003119 immunoblot Methods 0.000 description 20
- 239000000047 product Substances 0.000 description 19
- 238000003556 assay Methods 0.000 description 18
- 241000283973 Oryctolagus cuniculus Species 0.000 description 17
- 201000010099 disease Diseases 0.000 description 17
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 17
- 102000004196 processed proteins & peptides Human genes 0.000 description 17
- 239000006228 supernatant Substances 0.000 description 17
- 241000699666 Mus <mouse, genus> Species 0.000 description 16
- 230000000875 corresponding effect Effects 0.000 description 16
- 102100033994 Heterogeneous nuclear ribonucleoproteins C1/C2 Human genes 0.000 description 15
- 238000012512 characterization method Methods 0.000 description 15
- 238000006243 chemical reaction Methods 0.000 description 15
- 239000011521 glass Substances 0.000 description 15
- 230000019491 signal transduction Effects 0.000 description 15
- 108020004414 DNA Proteins 0.000 description 14
- 101001017574 Homo sapiens Heterogeneous nuclear ribonucleoproteins C1/C2 Proteins 0.000 description 14
- 101000941356 Nostoc ellipsosporum Cyanovirin-N Proteins 0.000 description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 238000010790 dilution Methods 0.000 description 13
- 239000012895 dilution Substances 0.000 description 13
- 241000283707 Capra Species 0.000 description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 238000007912 intraperitoneal administration Methods 0.000 description 12
- 239000013642 negative control Substances 0.000 description 12
- 238000003491 array Methods 0.000 description 11
- -1 but not limited to Proteins 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 11
- 102000037865 fusion proteins Human genes 0.000 description 11
- 108020001507 fusion proteins Proteins 0.000 description 11
- 238000002649 immunization Methods 0.000 description 11
- 239000003446 ligand Substances 0.000 description 11
- 229920001184 polypeptide Polymers 0.000 description 11
- 241000282693 Cercopithecidae Species 0.000 description 10
- 241000588724 Escherichia coli Species 0.000 description 10
- 241000282326 Felis catus Species 0.000 description 10
- 241000238631 Hexapoda Species 0.000 description 10
- 241000124008 Mammalia Species 0.000 description 10
- 241000009328 Perro Species 0.000 description 10
- 241000700159 Rattus Species 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 10
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 10
- 229910052760 oxygen Inorganic materials 0.000 description 10
- 239000002953 phosphate buffered saline Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 238000013459 approach Methods 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 9
- 230000003993 interaction Effects 0.000 description 9
- 206010003805 Autism Diseases 0.000 description 8
- 208000020706 Autistic disease Diseases 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 206010035226 Plasma cell myeloma Diseases 0.000 description 8
- 108091027967 Small hairpin RNA Proteins 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 239000013604 expression vector Substances 0.000 description 8
- 238000011534 incubation Methods 0.000 description 8
- 201000000050 myeloid neoplasm Diseases 0.000 description 8
- 229920000642 polymer Polymers 0.000 description 8
- 238000011176 pooling Methods 0.000 description 8
- 102000005962 receptors Human genes 0.000 description 8
- 108020003175 receptors Proteins 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 239000000872 buffer Substances 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 239000000284 extract Substances 0.000 description 7
- 210000002569 neuron Anatomy 0.000 description 7
- 238000010200 validation analysis Methods 0.000 description 7
- 206010021143 Hypoxia Diseases 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 6
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 6
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 210000000170 cell membrane Anatomy 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 230000004927 fusion Effects 0.000 description 6
- 230000007954 hypoxia Effects 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 210000004185 liver Anatomy 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 230000004960 subcellular localization Effects 0.000 description 6
- 239000013598 vector Substances 0.000 description 6
- 230000003612 virological effect Effects 0.000 description 6
- 208000002109 Argyria Diseases 0.000 description 5
- 108010078791 Carrier Proteins Proteins 0.000 description 5
- 230000005778 DNA damage Effects 0.000 description 5
- 231100000277 DNA damage Toxicity 0.000 description 5
- 108010019372 Heterogeneous-Nuclear Ribonucleoproteins Proteins 0.000 description 5
- 102000006479 Heterogeneous-Nuclear Ribonucleoproteins Human genes 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 230000002538 fungal effect Effects 0.000 description 5
- 210000005260 human cell Anatomy 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 210000001165 lymph node Anatomy 0.000 description 5
- 210000004379 membrane Anatomy 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 108020004999 messenger RNA Proteins 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 210000005253 yeast cell Anatomy 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 102100032187 Androgen receptor Human genes 0.000 description 4
- 206010003445 Ascites Diseases 0.000 description 4
- 102100025064 Cellular tumor antigen p53 Human genes 0.000 description 4
- 206010009944 Colon cancer Diseases 0.000 description 4
- 108090000079 Glucocorticoid Receptors Proteins 0.000 description 4
- 102100033417 Glucocorticoid receptor Human genes 0.000 description 4
- 101000721661 Homo sapiens Cellular tumor antigen p53 Proteins 0.000 description 4
- 206010029260 Neuroblastoma Diseases 0.000 description 4
- 108020005497 Nuclear hormone receptor Proteins 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 102000003728 Peroxisome Proliferator-Activated Receptors Human genes 0.000 description 4
- 108090000029 Peroxisome Proliferator-Activated Receptors Proteins 0.000 description 4
- 102100026123 Pirin Human genes 0.000 description 4
- 101710176373 Pirin Proteins 0.000 description 4
- 102000001253 Protein Kinase Human genes 0.000 description 4
- 208000006265 Renal cell carcinoma Diseases 0.000 description 4
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical group O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 4
- 238000001261 affinity purification Methods 0.000 description 4
- 125000003277 amino group Chemical group 0.000 description 4
- 108010080146 androgen receptors Proteins 0.000 description 4
- 210000000628 antibody-producing cell Anatomy 0.000 description 4
- 230000000890 antigenic effect Effects 0.000 description 4
- 230000031018 biological processes and functions Effects 0.000 description 4
- 229920001222 biopolymer Polymers 0.000 description 4
- 239000013592 cell lysate Substances 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 210000000172 cytosol Anatomy 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 102000015694 estrogen receptors Human genes 0.000 description 4
- 108010038795 estrogen receptors Proteins 0.000 description 4
- 238000005194 fractionation Methods 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 229930182830 galactose Natural products 0.000 description 4
- 238000001114 immunoprecipitation Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 4
- 210000003470 mitochondria Anatomy 0.000 description 4
- 230000010004 neural pathway Effects 0.000 description 4
- 102000006255 nuclear receptors Human genes 0.000 description 4
- 108020004017 nuclear receptors Proteins 0.000 description 4
- 229920002851 polycationic polymer Polymers 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000001742 protein purification Methods 0.000 description 4
- 239000012521 purified sample Substances 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000004055 small Interfering RNA Substances 0.000 description 4
- 230000002792 vascular Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 102100027211 Albumin Human genes 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 3
- 208000023275 Autoimmune disease Diseases 0.000 description 3
- 241000271566 Aves Species 0.000 description 3
- 108010017384 Blood Proteins Proteins 0.000 description 3
- 102000004506 Blood Proteins Human genes 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 241000282465 Canis Species 0.000 description 3
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 3
- 102000003849 Cytochrome P450 Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 3
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 3
- 241000283073 Equus caballus Species 0.000 description 3
- 241000282324 Felis Species 0.000 description 3
- 229930040373 Paraformaldehyde Natural products 0.000 description 3
- 108091000080 Phosphotransferase Proteins 0.000 description 3
- 241000288906 Primates Species 0.000 description 3
- 101710120037 Toxin CcdB Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 150000007513 acids Chemical class 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 230000005775 apoptotic pathway Effects 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000008235 cell cycle pathway Effects 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000009260 cross reactivity Effects 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000003197 gene knockdown Methods 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000016784 immunoglobulin production Effects 0.000 description 3
- 238000003364 immunohistochemistry Methods 0.000 description 3
- 239000012133 immunoprecipitate Substances 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000004879 molecular function Effects 0.000 description 3
- 235000019799 monosodium phosphate Nutrition 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 102000020233 phosphotransferase Human genes 0.000 description 3
- 108060006633 protein kinase Proteins 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 230000006798 recombination Effects 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 230000011664 signaling Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 150000003431 steroids Chemical class 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- NJYVEMPWNAYQQN-UHFFFAOYSA-N 5-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C21OC(=O)C1=CC(C(=O)O)=CC=C21 NJYVEMPWNAYQQN-UHFFFAOYSA-N 0.000 description 2
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 2
- 208000003174 Brain Neoplasms Diseases 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 2
- 208000026310 Breast neoplasm Diseases 0.000 description 2
- 101100257372 Caenorhabditis elegans sox-3 gene Proteins 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 2
- 206010008342 Cervix carcinoma Diseases 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 102100023580 Cyclic AMP-dependent transcription factor ATF-4 Human genes 0.000 description 2
- 102100022052 Cyclin N-terminal domain-containing protein 1 Human genes 0.000 description 2
- 102100023108 DPY30 domain-containing protein 2 Human genes 0.000 description 2
- 102100031418 EF-hand domain-containing protein D2 Human genes 0.000 description 2
- 206010014733 Endometrial cancer Diseases 0.000 description 2
- 206010014759 Endometrial neoplasm Diseases 0.000 description 2
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 2
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 2
- 101150075185 Foxp2 gene Proteins 0.000 description 2
- 102100038073 General transcription factor II-I Human genes 0.000 description 2
- 206010066476 Haematological malignancy Diseases 0.000 description 2
- 208000002250 Hematologic Neoplasms Diseases 0.000 description 2
- 108020004996 Heterogeneous Nuclear RNA Proteins 0.000 description 2
- 102100035081 Homeobox protein TGIF1 Human genes 0.000 description 2
- 101000905743 Homo sapiens Cyclic AMP-dependent transcription factor ATF-4 Proteins 0.000 description 2
- 101000900815 Homo sapiens Cyclin N-terminal domain-containing protein 1 Proteins 0.000 description 2
- 101001049997 Homo sapiens DPY30 domain-containing protein 2 Proteins 0.000 description 2
- 101000866913 Homo sapiens EF-hand domain-containing protein D2 Proteins 0.000 description 2
- 101001032427 Homo sapiens General transcription factor II-I Proteins 0.000 description 2
- 101000596925 Homo sapiens Homeobox protein TGIF1 Proteins 0.000 description 2
- 101001033233 Homo sapiens Interleukin-10 Proteins 0.000 description 2
- 101000634545 Homo sapiens Neuronal PAS domain-containing protein 3 Proteins 0.000 description 2
- 101000633503 Homo sapiens Nuclear receptor subfamily 2 group E member 1 Proteins 0.000 description 2
- 101000692980 Homo sapiens PHD finger protein 6 Proteins 0.000 description 2
- 101000664703 Homo sapiens Transcription factor SOX-10 Proteins 0.000 description 2
- 101000759565 Homo sapiens Zinc finger and BTB domain-containing protein 1 Proteins 0.000 description 2
- 101000760181 Homo sapiens Zinc finger protein 41 Proteins 0.000 description 2
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 2
- 108010058683 Immobilized Proteins Proteins 0.000 description 2
- 101150040658 LHX2 gene Proteins 0.000 description 2
- 108700042694 Lhx3 Proteins 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 102100025169 Max-binding protein MNT Human genes 0.000 description 2
- 101100257376 Mus musculus Sox3 gene Proteins 0.000 description 2
- 108010025020 Nerve Growth Factor Proteins 0.000 description 2
- 208000012902 Nervous system disease Diseases 0.000 description 2
- 208000025966 Neurological disease Diseases 0.000 description 2
- 102100029051 Neuronal PAS domain-containing protein 3 Human genes 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 102100029534 Nuclear receptor subfamily 2 group E member 1 Human genes 0.000 description 2
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 2
- 206010033128 Ovarian cancer Diseases 0.000 description 2
- 206010061535 Ovarian neoplasm Diseases 0.000 description 2
- 102100026365 PHD finger protein 6 Human genes 0.000 description 2
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 2
- 108010039918 Polylysine Proteins 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 206010060862 Prostate cancer Diseases 0.000 description 2
- 208000000236 Prostatic Neoplasms Diseases 0.000 description 2
- 102000044126 RNA-Binding Proteins Human genes 0.000 description 2
- 208000005718 Stomach Neoplasms Diseases 0.000 description 2
- 239000006180 TBST buffer Substances 0.000 description 2
- 102100038808 Transcription factor SOX-10 Human genes 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 2
- 102100023253 Zinc finger and BTB domain-containing protein 1 Human genes 0.000 description 2
- 102100024669 Zinc finger protein 41 Human genes 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000007818 agglutination assay Methods 0.000 description 2
- 125000003172 aldehyde group Chemical group 0.000 description 2
- 150000001412 amines Chemical group 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000008236 biological pathway Effects 0.000 description 2
- 239000000090 biomarker Substances 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 229910002091 carbon monoxide Inorganic materials 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 201000010881 cervical cancer Diseases 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 208000029742 colonic neoplasm Diseases 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000001212 derivatisation Methods 0.000 description 2
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 2
- 208000016097 disease of metabolism Diseases 0.000 description 2
- 238000010494 dissociation reaction Methods 0.000 description 2
- 230000005593 dissociations Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000009483 enzymatic pathway Effects 0.000 description 2
- 201000004101 esophageal cancer Diseases 0.000 description 2
- 210000002744 extracellular matrix Anatomy 0.000 description 2
- 230000008622 extracellular signaling Effects 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 206010017758 gastric cancer Diseases 0.000 description 2
- 201000011243 gastrointestinal stromal tumor Diseases 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000003102 growth factor Substances 0.000 description 2
- 201000010536 head and neck cancer Diseases 0.000 description 2
- 208000014829 head and neck neoplasm Diseases 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 206010073071 hepatocellular carcinoma Diseases 0.000 description 2
- 231100000844 hepatocellular carcinoma Toxicity 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000001597 immobilized metal affinity chromatography Methods 0.000 description 2
- 208000026278 immune system disease Diseases 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 208000027866 inflammatory disease Diseases 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 101150000168 lhx9 gene Proteins 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 201000001441 melanoma Diseases 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 229920000609 methyl cellulose Polymers 0.000 description 2
- 239000001923 methylcellulose Substances 0.000 description 2
- 238000010208 microarray analysis Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000002858 neurotransmitter agent Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 230000030648 nucleus localization Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 201000002528 pancreatic cancer Diseases 0.000 description 2
- 208000008443 pancreatic carcinoma Diseases 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 208000015754 perinatal disease Diseases 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 238000007747 plating Methods 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 2
- 229920000656 polylysine Polymers 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 229920006324 polyoxymethylene Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 238000002310 reflectometry Methods 0.000 description 2
- 238000007423 screening assay Methods 0.000 description 2
- 238000012883 sequential measurement Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 201000011549 stomach cancer Diseases 0.000 description 2
- WYWHKKSPHMUBEB-UHFFFAOYSA-N tioguanine Chemical compound N1C(N)=NC(=S)C2=C1N=CN2 WYWHKKSPHMUBEB-UHFFFAOYSA-N 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 230000004102 tricarboxylic acid cycle Effects 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical group CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
- BQCIDUSAKPWEOX-UHFFFAOYSA-N 1,1-Difluoroethene Chemical compound FC(F)=C BQCIDUSAKPWEOX-UHFFFAOYSA-N 0.000 description 1
- WQZIDRAQTRIQDX-UHFFFAOYSA-N 6-carboxy-x-rhodamine Chemical compound OC(=O)C1=CC=C(C([O-])=O)C=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 WQZIDRAQTRIQDX-UHFFFAOYSA-N 0.000 description 1
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 description 1
- 229960005508 8-azaguanine Drugs 0.000 description 1
- 101150092476 ABCA1 gene Proteins 0.000 description 1
- 101150013107 ARNT2 gene Proteins 0.000 description 1
- 102000055510 ATP Binding Cassette Transporter 1 Human genes 0.000 description 1
- 108700005241 ATP Binding Cassette Transporter 1 Proteins 0.000 description 1
- 102000005416 ATP-Binding Cassette Transporters Human genes 0.000 description 1
- 108010006533 ATP-Binding Cassette Transporters Proteins 0.000 description 1
- 101710159080 Aconitate hydratase A Proteins 0.000 description 1
- 101710159078 Aconitate hydratase B Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 208000009575 Angelman syndrome Diseases 0.000 description 1
- JBRZTFJDHDCESZ-UHFFFAOYSA-N AsGa Chemical compound [As]#[Ga] JBRZTFJDHDCESZ-UHFFFAOYSA-N 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 101150035467 BDNF gene Proteins 0.000 description 1
- COXVTLYNGOIATD-HVMBLDELSA-N CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O Chemical compound CC1=C(C=CC(=C1)C1=CC(C)=C(C=C1)\N=N\C1=C(O)C2=C(N)C(=CC(=C2C=C1)S(O)(=O)=O)S(O)(=O)=O)\N=N\C1=CC=C2C(=CC(=C(N)C2=C1O)S(O)(=O)=O)S(O)(=O)=O COXVTLYNGOIATD-HVMBLDELSA-N 0.000 description 1
- 101100055523 Caenorhabditis elegans amt-2 gene Proteins 0.000 description 1
- 101100518995 Caenorhabditis elegans pax-3 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 102100027047 Cell division control protein 6 homolog Human genes 0.000 description 1
- 102000001327 Chemokine CCL5 Human genes 0.000 description 1
- 108010055166 Chemokine CCL5 Proteins 0.000 description 1
- 102000019034 Chemokines Human genes 0.000 description 1
- 108010012236 Chemokines Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 description 1
- 102100024829 DNA polymerase delta catalytic subunit Human genes 0.000 description 1
- 102100037458 Dephospho-CoA kinase Human genes 0.000 description 1
- 101000876610 Dictyostelium discoideum Extracellular signal-regulated kinase 2 Proteins 0.000 description 1
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 101150059079 EBNA1 gene Proteins 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102400001368 Epidermal growth factor Human genes 0.000 description 1
- 101800003838 Epidermal growth factor Proteins 0.000 description 1
- 102100026541 F-actin-capping protein subunit alpha-3 Human genes 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229910001218 Gallium arsenide Inorganic materials 0.000 description 1
- 102100021792 Gamma-sarcoglycan Human genes 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 108010024636 Glutathione Proteins 0.000 description 1
- 102000005720 Glutathione transferase Human genes 0.000 description 1
- 108010070675 Glutathione transferase Proteins 0.000 description 1
- 102100039996 Histone deacetylase 1 Human genes 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 102100027345 Homeobox protein SIX3 Human genes 0.000 description 1
- 101000914465 Homo sapiens Cell division control protein 6 homolog Proteins 0.000 description 1
- 101000868333 Homo sapiens Cyclin-dependent kinase 1 Proteins 0.000 description 1
- 101000909198 Homo sapiens DNA polymerase delta catalytic subunit Proteins 0.000 description 1
- 101000952691 Homo sapiens Dephospho-CoA kinase Proteins 0.000 description 1
- 101000983575 Homo sapiens F-actin-capping protein subunit alpha-3 Proteins 0.000 description 1
- 101000616435 Homo sapiens Gamma-sarcoglycan Proteins 0.000 description 1
- 101001035024 Homo sapiens Histone deacetylase 1 Proteins 0.000 description 1
- 101000651928 Homo sapiens Homeobox protein SIX3 Proteins 0.000 description 1
- 101001053263 Homo sapiens Insulin gene enhancer protein ISL-1 Proteins 0.000 description 1
- 101001052493 Homo sapiens Mitogen-activated protein kinase 1 Proteins 0.000 description 1
- 101001109698 Homo sapiens Nuclear receptor subfamily 4 group A member 2 Proteins 0.000 description 1
- 101001069723 Homo sapiens Paired mesoderm homeobox protein 2 Proteins 0.000 description 1
- 101000692768 Homo sapiens Paired mesoderm homeobox protein 2B Proteins 0.000 description 1
- 101001067178 Homo sapiens Plexin-A4 Proteins 0.000 description 1
- 101000735354 Homo sapiens Poly(rC)-binding protein 1 Proteins 0.000 description 1
- 101000760185 Homo sapiens Putative zinc finger protein 726P1 Proteins 0.000 description 1
- 101001132698 Homo sapiens Retinoic acid receptor beta Proteins 0.000 description 1
- 101000688996 Homo sapiens Ski-like protein Proteins 0.000 description 1
- 101000796028 Homo sapiens Thioredoxin domain-containing protein 9 Proteins 0.000 description 1
- 101001065732 Homo sapiens U6 snRNA-associated Sm-like protein LSm6 Proteins 0.000 description 1
- 101000743803 Homo sapiens Zinc finger protein 674 Proteins 0.000 description 1
- 101000964727 Homo sapiens Zinc finger protein 74 Proteins 0.000 description 1
- 101000964787 Homo sapiens Zinc finger protein 80 Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000004286 Hydroxymethylglutaryl CoA Reductases Human genes 0.000 description 1
- 108090000895 Hydroxymethylglutaryl CoA Reductases Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102100023915 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102100024392 Insulin gene enhancer protein ISL-1 Human genes 0.000 description 1
- 102000008070 Interferon-gamma Human genes 0.000 description 1
- 108010074328 Interferon-gamma Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000004310 Ion Channels Human genes 0.000 description 1
- 101150007623 Isl2 gene Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 101150083522 MECP2 gene Proteins 0.000 description 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 description 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 description 1
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 208000036626 Mental retardation Diseases 0.000 description 1
- 102100025825 Methylated-DNA-protein-cysteine methyltransferase Human genes 0.000 description 1
- 101710169105 Minor spike protein Proteins 0.000 description 1
- 101710081079 Minor spike protein H Proteins 0.000 description 1
- 108010058682 Mitochondrial Proteins Proteins 0.000 description 1
- 102000006404 Mitochondrial Proteins Human genes 0.000 description 1
- 102100024193 Mitogen-activated protein kinase 1 Human genes 0.000 description 1
- 208000019022 Mood disease Diseases 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 101100518997 Mus musculus Pax3 gene Proteins 0.000 description 1
- KWYHDKDOAIKMQN-UHFFFAOYSA-N N,N,N',N'-tetramethylethylenediamine Chemical compound CN(C)CCN(C)C KWYHDKDOAIKMQN-UHFFFAOYSA-N 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 1
- 108091007369 NEUR proteins Proteins 0.000 description 1
- 102000015336 Nerve Growth Factor Human genes 0.000 description 1
- 102000007072 Nerve Growth Factors Human genes 0.000 description 1
- 102100030466 Neurocan core protein Human genes 0.000 description 1
- 101100457407 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) mmm-1 gene Proteins 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 102100022676 Nuclear receptor subfamily 4 group A member 2 Human genes 0.000 description 1
- 108050002069 Olfactory receptors Proteins 0.000 description 1
- 102000012547 Olfactory receptors Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 102100033829 Paired mesoderm homeobox protein 2 Human genes 0.000 description 1
- 102100026354 Paired mesoderm homeobox protein 2B Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000577979 Peromyscus spicilegus Species 0.000 description 1
- 108010089430 Phosphoproteins Proteins 0.000 description 1
- 102000007982 Phosphoproteins Human genes 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 101150016832 Pias3 gene Proteins 0.000 description 1
- 102100034385 Plexin-A4 Human genes 0.000 description 1
- 102100034960 Poly(rC)-binding protein 1 Human genes 0.000 description 1
- 229920001665 Poly-4-vinylphenol Polymers 0.000 description 1
- 229920002873 Polyethylenimine Polymers 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 201000010769 Prader-Willi syndrome Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010015499 Protein Kinase C-theta Proteins 0.000 description 1
- 102100021566 Protein kinase C theta type Human genes 0.000 description 1
- 102100024664 Putative zinc finger protein 726P1 Human genes 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 108700020471 RNA-Binding Proteins Proteins 0.000 description 1
- 101710105008 RNA-binding protein Proteins 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 201000007737 Retinal degeneration Diseases 0.000 description 1
- 102100033909 Retinoic acid receptor beta Human genes 0.000 description 1
- 208000006289 Rett Syndrome Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 102000037054 SLC-Transporter Human genes 0.000 description 1
- 108091006207 SLC-Transporter Proteins 0.000 description 1
- 101150068315 Scn2a gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108010034546 Serratia marcescens nuclease Proteins 0.000 description 1
- 229910052581 Si3N4 Inorganic materials 0.000 description 1
- 102100024451 Ski-like protein Human genes 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 244000046101 Sophora japonica Species 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 102100031350 Thioredoxin domain-containing protein 9 Human genes 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 206010052779 Transplant rejections Diseases 0.000 description 1
- 102100032068 U6 snRNA-associated Sm-like protein LSm6 Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 206010049644 Williams syndrome Diseases 0.000 description 1
- 201000001305 Williams-Beuren syndrome Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 102100039040 Zinc finger protein 674 Human genes 0.000 description 1
- 102100040711 Zinc finger protein 74 Human genes 0.000 description 1
- 102100040642 Zinc finger protein 80 Human genes 0.000 description 1
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 1
- 229960004373 acetylcholine Drugs 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 108010004469 allophycocyanin Proteins 0.000 description 1
- 229910045601 alloy Inorganic materials 0.000 description 1
- 239000000956 alloy Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
- VEQOALNAAJBPNY-UHFFFAOYSA-N antipyrine Chemical compound CN1C(C)=CC(=O)N1C1=CC=CC=C1 VEQOALNAAJBPNY-UHFFFAOYSA-N 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 101150010487 are gene Proteins 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 230000035578 autophosphorylation Effects 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000009134 cell regulation Effects 0.000 description 1
- 230000036755 cellular response Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000005289 controlled pore glass Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000003544 deproteinization Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000001085 differential centrifugation Methods 0.000 description 1
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 1
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 1
- 239000004205 dimethyl polysiloxane Substances 0.000 description 1
- 208000022602 disease susceptibility Diseases 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000008482 dysregulation Effects 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940116977 epidermal growth factor Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229960003699 evans blue Drugs 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000011990 functional testing Methods 0.000 description 1
- 230000000799 fusogenic effect Effects 0.000 description 1
- 238000012817 gel-diffusion technique Methods 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 230000004110 gluconeogenesis Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 108091005708 gustatory receptors Proteins 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 108091008039 hormone receptors Proteins 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000000899 immune system response Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000000951 immunodiffusion Effects 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000003017 in situ immunoassay Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 229960003130 interferon gamma Drugs 0.000 description 1
- 210000004020 intracellular membrane Anatomy 0.000 description 1
- 230000031146 intracellular signal transduction Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000003368 label free method Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 230000037356 lipid metabolism Effects 0.000 description 1
- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 229960003987 melatonin Drugs 0.000 description 1
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 108040008770 methylated-DNA-[protein]-cysteine S-methyltransferase activity proteins Proteins 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 210000002850 nasal mucosa Anatomy 0.000 description 1
- 230000009826 neoplastic cell growth Effects 0.000 description 1
- 229940053128 nerve growth factor Drugs 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 101150017648 neurod2 gene Proteins 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 102000044158 nucleic acid binding protein Human genes 0.000 description 1
- 108700020942 nucleic acid binding protein Proteins 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 230000010627 oxidative phosphorylation Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000004108 pentose phosphate pathway Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 108091008695 photoreceptors Proteins 0.000 description 1
- 229920002120 photoresistant polymer Polymers 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 1
- 229920000747 poly(lactic acid) Polymers 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 108010011110 polyarginine Proteins 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920002530 polyetherether ketone Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920002338 polyhydroxyethylmethacrylate Polymers 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 150000004032 porphyrins Chemical class 0.000 description 1
- 230000002516 postimmunization Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000013197 protein A assay Methods 0.000 description 1
- 238000003498 protein array Methods 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 230000006825 purine synthesis Effects 0.000 description 1
- 230000006824 pyrimidine synthesis Effects 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000028617 response to DNA damage stimulus Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 210000001995 reticulocyte Anatomy 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 230000004258 retinal degeneration Effects 0.000 description 1
- 210000000880 retinal rod photoreceptor cell Anatomy 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 201000000980 schizophrenia Diseases 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 150000004756 silanes Chemical class 0.000 description 1
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 101150077014 sox10 gene Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 238000012289 standard assay Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- KZNICNPSHKQLFF-UHFFFAOYSA-N succinimide Chemical group O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 description 1
- 230000010741 sumoylation Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 210000001779 taste bud Anatomy 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 229960003087 tioguanine Drugs 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 108091006107 transcriptional repressors Proteins 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000012250 transgenic expression Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- QQQSFSZALRVCSZ-UHFFFAOYSA-N triethoxysilane Chemical compound CCO[SiH](OCC)OCC QQQSFSZALRVCSZ-UHFFFAOYSA-N 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000013024 troubleshooting Methods 0.000 description 1
- 230000004143 urea cycle Effects 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35532910P | 2010-06-16 | 2010-06-16 | |
| US61/355,329 | 2010-06-16 | ||
| PCT/US2011/040789 WO2011159959A2 (en) | 2010-06-16 | 2011-06-16 | Methods and systems for generating, validating and using monoclonal antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2802868A1 true CA2802868A1 (en) | 2011-12-22 |
Family
ID=45348882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2802868A Abandoned CA2802868A1 (en) | 2010-06-16 | 2011-06-16 | Methods and systems for generating, validating and using monoclonal antibodies |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US20130288910A1 (enExample) |
| EP (1) | EP2582862B1 (enExample) |
| JP (2) | JP2013533865A (enExample) |
| CN (1) | CN103168119B (enExample) |
| CA (1) | CA2802868A1 (enExample) |
| WO (1) | WO2011159959A2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140051586A1 (en) * | 2011-01-25 | 2014-02-20 | Ignacio Pino | High-throughput methods to produce, validate and characterize mmabs |
| US20140307931A1 (en) * | 2013-04-15 | 2014-10-16 | Massachusetts Institute Of Technology | Fully automated system and method for image segmentation and quality control of protein microarrays |
| WO2014205344A2 (en) * | 2013-06-21 | 2014-12-24 | The Johns Hopkins University | Virion display array for profiling functions and interactions of human membrane proteins |
| WO2017177140A1 (en) * | 2016-04-07 | 2017-10-12 | Rowan University | Methods and kits for detecting myo/nog cells in human adipose tissue and methods of use thereof |
| CN109470852A (zh) * | 2018-12-07 | 2019-03-15 | 华南农业大学 | 一种母乳多克隆抗体免疫亲和柱及其制备方法 |
| WO2025101487A1 (en) * | 2023-11-06 | 2025-05-15 | Bruker Cellular Analysis, Inc. | Affinity assay for microfluidic devices |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4134297A1 (de) * | 1991-10-17 | 1993-04-22 | Behringwerke Ag | Monoclonale antikoerper gegen mycoplasma pneumoniae, diese produzierende hybridome, verfahren zu deren herstellung sowie deren verwendung |
| EP1051624A4 (en) * | 1998-01-29 | 2002-05-02 | Glaucus Proteomics B V | HIGH DENSITY MATERIALS FOR PROTEOM ANALYSIS AND METHOD AND COMPOSITIONS THEREFOR |
| US6794132B2 (en) * | 1999-10-02 | 2004-09-21 | Biosite, Inc. | Human antibodies |
| EP1572971B1 (en) * | 2002-11-15 | 2009-09-30 | Morphotek Inc. | Methods of generating high-production of antibodies from hybridomas created by in vitro immunization |
| WO2006033972A2 (en) * | 2004-09-15 | 2006-03-30 | Protometrix, Inc. | Protein arrays and methods of use thereof |
| WO2006043179A1 (en) * | 2004-10-23 | 2006-04-27 | Biosystems International Sas | Expression profiling platform technology |
| BRPI0518734A2 (pt) * | 2004-11-30 | 2008-12-02 | Veridex Llc | prognàstico de cÂncer de pulmço |
| WO2006124644A2 (en) * | 2005-05-12 | 2006-11-23 | Board Of Regents, The University Of Texas System | Protein and antibody profiling using small molecule microarrays |
| CN1786298A (zh) * | 2005-10-28 | 2006-06-14 | 上海博昂抗体生物科技有限公司 | 抗细胞或组织蛋白质单克隆抗体库的制备方法 |
| EP1921142A1 (en) * | 2006-11-07 | 2008-05-14 | Cytos Biotechnology AG | Selection of human monoclonal antibodies by eukaryotic cell display |
| US20090305899A1 (en) * | 2007-11-16 | 2009-12-10 | Invitrogen Incorporated | Compositions and methods for determining immune status |
| WO2010011337A1 (en) * | 2008-07-25 | 2010-01-28 | Theraclone Sciences | Methods and compositions for discovery of target-specific antibodies using antibody repertoire array (ara) |
| BR112012006678A2 (pt) * | 2009-09-25 | 2016-05-10 | Origene Technologies Inc | método para criar um arranjo proteico, arranjo proteico e métodos para determinar a especificidade da ligação de um anticorpo ou preparação de anticorpos, para determinar biomarcadores de doenças, para diagnosticar uma doença, para monitorar as vias de transdução de sinais e para determinar interações entre pequenas moléculas e proteínas |
-
2011
- 2011-06-16 US US13/704,592 patent/US20130288910A1/en not_active Abandoned
- 2011-06-16 EP EP11796472.6A patent/EP2582862B1/en active Active
- 2011-06-16 JP JP2013515529A patent/JP2013533865A/ja not_active Withdrawn
- 2011-06-16 CN CN201180039055.8A patent/CN103168119B/zh active Active
- 2011-06-16 WO PCT/US2011/040789 patent/WO2011159959A2/en not_active Ceased
- 2011-06-16 CA CA2802868A patent/CA2802868A1/en not_active Abandoned
-
2016
- 2016-10-03 JP JP2016195881A patent/JP2016222725A/ja active Pending
-
2017
- 2017-06-23 US US15/632,261 patent/US20180030086A1/en not_active Abandoned
-
2022
- 2022-06-06 US US17/833,001 patent/US20220363712A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| HK1182745A1 (zh) | 2013-12-06 |
| WO2011159959A2 (en) | 2011-12-22 |
| US20220363712A1 (en) | 2022-11-17 |
| US20130288910A1 (en) | 2013-10-31 |
| EP2582862B1 (en) | 2016-04-13 |
| US20180030086A1 (en) | 2018-02-01 |
| EP2582862A4 (en) | 2014-01-15 |
| CN103168119A (zh) | 2013-06-19 |
| WO2011159959A3 (en) | 2013-03-28 |
| JP2013533865A (ja) | 2013-08-29 |
| CN103168119B (zh) | 2016-05-11 |
| JP2016222725A (ja) | 2016-12-28 |
| EP2582862A2 (en) | 2013-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20220363712A1 (en) | Methods and systems for generating, validating and using monoclonal antibodies | |
| US10175249B2 (en) | Proteomic identification of antibodies | |
| US11421347B2 (en) | Methods for labelling, analyzing, detecting and measuring protein-protein interactions | |
| US20140256579A1 (en) | Protein Arrays | |
| CN1726394B (zh) | 针对短表位的数字化抗体组以及其使用方法 | |
| US20080241934A1 (en) | Methods for reducing complexity of a sample using small epitope antibodies | |
| US20140051586A1 (en) | High-throughput methods to produce, validate and characterize mmabs | |
| CN107383199B (zh) | 一种s-腺苷蛋氨酸合成酶的单克隆抗体及其应用 | |
| Kothiwal et al. | High-Throughput Machine Learning-Aided Antibody Discovery for Cell Surface Antigens | |
| HK1182745B (en) | Methods and systems for generating, validating and using monoclonal antibodies | |
| US20140357519A1 (en) | Methods of screening monoclonal antibody populations | |
| Saragozza | High-throughput antibody validation platforms | |
| Boeke et al. | Rapid Identification of Monospecific Monoclonal Antibodies Using a Human Proteome Microarray |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20160615 |
|
| FZDE | Discontinued |
Effective date: 20181029 |