CN103154233A - 生产蛋白质的方法 - Google Patents

生产蛋白质的方法 Download PDF

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Publication number
CN103154233A
CN103154233A CN2011800482244A CN201180048224A CN103154233A CN 103154233 A CN103154233 A CN 103154233A CN 2011800482244 A CN2011800482244 A CN 2011800482244A CN 201180048224 A CN201180048224 A CN 201180048224A CN 103154233 A CN103154233 A CN 103154233A
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China
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protein
cell culture
suspension
culture
cell
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Pending
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CN2011800482244A
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English (en)
Chinese (zh)
Inventor
J.罗班
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Novo Nordisk Health Care AG
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Novo Nordisk Health Care AG
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43735957&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN103154233(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk Health Care AG filed Critical Novo Nordisk Health Care AG
Priority to CN201710499437.8A priority Critical patent/CN107190034A/zh
Publication of CN103154233A publication Critical patent/CN103154233A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN2011800482244A 2010-10-05 2011-10-05 生产蛋白质的方法 Pending CN103154233A (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710499437.8A CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP10186545.9 2010-10-05
EP10186545 2010-10-05
US39271310P 2010-10-13 2010-10-13
US61/392713 2010-10-13
PCT/EP2011/067372 WO2012045769A1 (en) 2010-10-05 2011-10-05 Process for protein production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201710499437.8A Division CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

Publications (1)

Publication Number Publication Date
CN103154233A true CN103154233A (zh) 2013-06-12

Family

ID=43735957

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2011800482244A Pending CN103154233A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法
CN201710499437.8A Pending CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

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CN201710499437.8A Pending CN107190034A (zh) 2010-10-05 2011-10-05 生产蛋白质的方法

Country Status (7)

Country Link
US (1) US10138290B2 (cg-RX-API-DMAC7.html)
EP (2) EP2625262B1 (cg-RX-API-DMAC7.html)
JP (2) JP6282467B2 (cg-RX-API-DMAC7.html)
CN (2) CN103154233A (cg-RX-API-DMAC7.html)
ES (1) ES2556454T3 (cg-RX-API-DMAC7.html)
PL (1) PL2625262T3 (cg-RX-API-DMAC7.html)
WO (1) WO2012045769A1 (cg-RX-API-DMAC7.html)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105579572A (zh) * 2013-09-30 2016-05-11 克鲁塞尔荷兰公司 用于净化高密度粗细胞培养收获物的方法
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用
CN112313323A (zh) * 2018-05-04 2021-02-02 建新公司 具有过滤系统的灌注式生物反应器

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015526094A (ja) * 2012-08-20 2015-09-10 テルモ ビーシーティー、インコーポレーテッド 細胞成長チャンバーを循環する流体の成分を濃縮する方法
JP6530171B2 (ja) * 2014-09-09 2019-06-12 旭化成メディカル株式会社 培養産生物の回収方法
AU2017301691B2 (en) * 2016-07-25 2022-07-21 Repligen Corporation Alternating tangential flow rapid harvesting
CN111263631A (zh) * 2017-10-26 2020-06-09 瑞普利金公司 微型交替切向流灌注过滤器、处理容器及其使用方法
CN114127260A (zh) * 2019-07-16 2022-03-01 信达生物制药(苏州)有限公司 高密度连续接种的细胞培养方法及其应用

Citations (3)

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WO2005095578A1 (en) * 2004-03-05 2005-10-13 Dsm Ip Assets B.V. Process for cell culturing by continuous perfusion and alternating tangential flow
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
WO2009130198A2 (en) * 2008-04-21 2009-10-29 Novo Nordisk A/S Hyperglycosylated human coagulation factor ix

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EP0253870B1 (en) 1986-01-03 1993-03-31 Genetics Institute, Inc. Method for producing factor viii:c-type proteins
CA1331157C (en) 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
US4956080A (en) * 1987-08-03 1990-09-11 Microlift Systems, Incorporated High pressure oxygen-saturated water treatment apparatus
JPH066054B2 (ja) 1987-10-15 1994-01-26 帝人株式会社 動物細胞の培養方法
JPH084495B2 (ja) 1988-08-19 1996-01-24 帝人株式会社 接着性動物細胞の培養方法
WO1990002175A1 (en) 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
US6268487B1 (en) 1996-05-13 2001-07-31 Genzyme Transgenics Corporation Purification of biologically active peptides from milk
US5851800A (en) 1996-05-14 1998-12-22 Pharmacia & Upjohn Ab Process for producing a protein
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
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US7358067B2 (en) 2000-10-20 2008-04-15 Bioneer A/S Fermentation method for production of heterologous gene products in lactic acid bacteria
ES2490590T3 (es) 2001-11-02 2014-09-04 Novo Nordisk Health Care Ag Polipéptidos de factor VII de coagulación humana
US6960657B2 (en) 2001-11-02 2005-11-01 Novo Nordisk Healthcare A/G Human coagulation factor VII polypeptides
DK1517710T3 (da) 2002-06-21 2011-07-18 Novo Nordisk Healthcare Ag Pegylerede faktor VII-glycoformer
US7351688B2 (en) 2003-02-05 2008-04-01 The Research Foundation Of State University Of New York Compositions and methods for less immunogenic protein formulations
CN100432217C (zh) 2003-05-01 2008-11-12 帝斯曼知识产权资产管理有限公司 通过对悬浮的动物细胞进行灌流式培养来生产生物物质的方法
CN101328473B (zh) 2003-05-09 2012-05-02 克鲁塞尔荷兰公司 E1-永生化的细胞培养物及培养所述细胞以提高从中获取的产物产量的方法
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CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
WO2009130198A2 (en) * 2008-04-21 2009-10-29 Novo Nordisk A/S Hyperglycosylated human coagulation factor ix

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105579572A (zh) * 2013-09-30 2016-05-11 克鲁塞尔荷兰公司 用于净化高密度粗细胞培养收获物的方法
CN112313323A (zh) * 2018-05-04 2021-02-02 建新公司 具有过滤系统的灌注式生物反应器
CN112313323B (zh) * 2018-05-04 2024-03-29 建新公司 具有过滤系统的灌注式生物反应器
CN108504562A (zh) * 2018-06-21 2018-09-07 江苏澳创生物科技有限公司 一种发酵生产l-苏氨酸的系统及其应用

Also Published As

Publication number Publication date
US20130244283A1 (en) 2013-09-19
JP6282467B2 (ja) 2018-02-21
EP2957628A1 (en) 2015-12-23
JP2013538588A (ja) 2013-10-17
EP2625262B1 (en) 2015-09-23
US10138290B2 (en) 2018-11-27
EP2625262A1 (en) 2013-08-14
CN107190034A (zh) 2017-09-22
WO2012045769A1 (en) 2012-04-12
PL2625262T3 (pl) 2016-05-31
ES2556454T3 (es) 2016-01-18
JP2016187360A (ja) 2016-11-04

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Application publication date: 20130612