CN103146572B - 一种实现细胞集落均质性生长的装置和方法 - Google Patents

一种实现细胞集落均质性生长的装置和方法 Download PDF

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CN103146572B
CN103146572B CN201110403987.8A CN201110403987A CN103146572B CN 103146572 B CN103146572 B CN 103146572B CN 201110403987 A CN201110403987 A CN 201110403987A CN 103146572 B CN103146572 B CN 103146572B
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gelatin
cell colony
culture device
cell
vitro culture
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CN103146572A (zh
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杜亚楠
姚睿
王婧宇
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Tsinghua University
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Priority to IN1409KON2014 priority patent/IN2014KN01409A/en
Priority to JP2014545085A priority patent/JP2015501640A/ja
Priority to PCT/CN2012/086149 priority patent/WO2013083073A1/zh
Priority to EP12855702.2A priority patent/EP2796541A1/en
Priority to US14/363,507 priority patent/US20140295553A1/en
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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CN201110403987.8A 2011-12-07 2011-12-07 一种实现细胞集落均质性生长的装置和方法 Expired - Fee Related CN103146572B (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN201110403987.8A CN103146572B (zh) 2011-12-07 2011-12-07 一种实现细胞集落均质性生长的装置和方法
IN1409KON2014 IN2014KN01409A (enExample) 2011-12-07 2012-12-07
JP2014545085A JP2015501640A (ja) 2011-12-07 2012-12-07 細胞コロニーの体外培養装置及びその使用
PCT/CN2012/086149 WO2013083073A1 (zh) 2011-12-07 2012-12-07 细胞集落体外培养装置及其用途
EP12855702.2A EP2796541A1 (en) 2011-12-07 2012-12-07 In-vitro cell colony cultivation device and use thereof
US14/363,507 US20140295553A1 (en) 2011-12-07 2012-12-07 In-vitro cell colony culture device and use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201110403987.8A CN103146572B (zh) 2011-12-07 2011-12-07 一种实现细胞集落均质性生长的装置和方法

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CN103146572B true CN103146572B (zh) 2016-01-06

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US (1) US20140295553A1 (enExample)
EP (1) EP2796541A1 (enExample)
JP (1) JP2015501640A (enExample)
CN (1) CN103146572B (enExample)
IN (1) IN2014KN01409A (enExample)
WO (1) WO2013083073A1 (enExample)

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CN103656750B (zh) * 2013-12-07 2014-12-24 西南交通大学 一种在心血管植入材料表面改善多种仿生功能的方法
CN103898055B (zh) * 2014-01-20 2017-01-11 复旦大学附属中山医院 不同基底硬度体外细胞培养平台的建立方法
US10195313B2 (en) * 2014-04-10 2019-02-05 Wisconsin Alumni Research Foundation Method for forming hydrogel arrays using surfaces with differential wettability
JP6817194B2 (ja) * 2014-05-12 2021-01-20 ルースタービオ インコーポレイテッド 即時プリント可能な細胞及び一体化されたデバイス
US10808221B2 (en) 2014-12-22 2020-10-20 Corning Incorporated Proteinase-free coatings for colony passaging
EP3320081A1 (en) 2015-07-08 2018-05-16 Paris Sciences et Lettres - Quartier Latin Cell culture device
CN105294955B (zh) * 2015-10-23 2018-04-24 华中科技大学 一种用于多细胞分选和干细胞选区分化的光固化水凝胶及其制备方法
CN106609242B (zh) * 2015-10-26 2020-01-21 泰陞国际科技股份有限公司 细胞承载装置及细胞培养系统
US11186810B2 (en) * 2016-03-16 2021-11-30 Shimadzu Corporation Cell culture vessel
MA44490A (fr) * 2016-03-22 2019-01-30 Harvard College Adhésifs biocompatibles et leurs procédés d'utilisation
CN106754365B (zh) * 2016-12-01 2019-06-14 天津市第一中心医院 一种缓释生长因子多细胞球培养膜板及其构建方法
JP6721881B2 (ja) * 2017-02-23 2020-07-15 日本電信電話株式会社 細胞足場材料、細胞モデル、及び細胞足場材料の製造方法
KR101989468B1 (ko) * 2017-06-22 2019-06-14 성균관대학교 산학협력단 줄기세포 배양용 기판의 제조방법
JPWO2019078278A1 (ja) * 2017-10-18 2020-11-05 国立大学法人京都大学 心筋細胞に分化させるための多能性幹細胞の製造方法
CN107764716B (zh) * 2017-10-21 2020-08-14 云南中烟工业有限责任公司 一种卷烟烟气对细胞水渗透性影响的检测方法
CN107854218A (zh) * 2017-12-12 2018-03-30 成都美益达医疗科技有限公司 一种一次性使用的水胶体抗菌敷贴
CN108078676A (zh) * 2017-12-12 2018-05-29 成都美益达医疗科技有限公司 一种治疗关节部位伤口的敷贴
FR3081168B1 (fr) * 2018-05-21 2022-03-11 Univ Bordeaux Systeme de culture cellulaire en bioreacteur
CN108949524B (zh) * 2018-07-26 2021-10-08 上海大学 用于体外细胞三维微组织形成的笼结构微孔培养皿及其制备方法
CN109730811B (zh) * 2018-12-17 2021-07-13 中国科学院上海硅酸盐研究所 仿骨哈弗斯系统生物活性支架及其制备方法和应用
CN109628390A (zh) * 2018-12-30 2019-04-16 深圳光彩生命工程技术有限公司 一种水凝胶状的细胞培养配方
JPWO2020203769A1 (enExample) * 2019-03-29 2020-10-08
CN111621468A (zh) * 2020-05-21 2020-09-04 上海慧灯医疗科技有限公司 一种制备诱导多能干细胞源性心肌细胞的方法及其固定方法和应用
CN113930337B (zh) * 2020-06-29 2023-03-17 清华大学 用于制备细胞团簇的装置及其构建方法及应用
CN111748475A (zh) * 2020-07-14 2020-10-09 兰州大学第一医院 一种流体细胞培养装置
JP7635934B2 (ja) * 2021-03-03 2025-02-26 国立大学法人 東京大学 評価用器具及び評価方法
CN113995444B (zh) * 2021-11-03 2024-05-07 深圳大学 一种水凝胶微针贴片及其制备方法与应用

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IN2014KN01409A (enExample) 2015-10-23
EP2796541A1 (en) 2014-10-29
CN103146572A (zh) 2013-06-12
JP2015501640A (ja) 2015-01-19
US20140295553A1 (en) 2014-10-02
WO2013083073A1 (zh) 2013-06-13

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