IN2014KN01409A - - Google Patents

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Publication number
IN2014KN01409A
IN2014KN01409A IN1409KON2014A IN2014KN01409A IN 2014KN01409 A IN2014KN01409 A IN 2014KN01409A IN 1409KON2014 A IN1409KON2014 A IN 1409KON2014A IN 2014KN01409 A IN2014KN01409 A IN 2014KN01409A
Authority
IN
India
Prior art keywords
micro
cell colony
cell
cultivation device
micro pattern
Prior art date
Application number
Inventor
Yanan Du
Rui Yao
Jingyu Wang
Original Assignee
Univ Tsinghua
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Tsinghua filed Critical Univ Tsinghua
Publication of IN2014KN01409A publication Critical patent/IN2014KN01409A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Clinical Laboratory Science (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Materials For Medical Uses (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Provided are an in vitro cell colony cultivation device and use thereof the in vitro cell colony cultivation device comprising: a micro patterned template having a micro pattern cavity and adhesive hydrogel formed on the lower surface of the micro patterned template the micro pattern cavity defining the growth space of the cell colony. An upper template can be retained or removed as required to form a monolayer cell micro pattern or a multilayer cell micro cluster.
IN1409KON2014 2011-12-07 2012-12-07 IN2014KN01409A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201110403987.8A CN103146572B (en) 2011-12-07 2011-12-07 A kind of apparatus and method realizing the growth of cell colony uniformity
PCT/CN2012/086149 WO2013083073A1 (en) 2011-12-07 2012-12-07 In-vitro cell colony cultivation device and use thereof

Publications (1)

Publication Number Publication Date
IN2014KN01409A true IN2014KN01409A (en) 2015-10-23

Family

ID=48544896

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1409KON2014 IN2014KN01409A (en) 2011-12-07 2012-12-07

Country Status (6)

Country Link
US (1) US20140295553A1 (en)
EP (1) EP2796541A1 (en)
JP (1) JP2015501640A (en)
CN (1) CN103146572B (en)
IN (1) IN2014KN01409A (en)
WO (1) WO2013083073A1 (en)

Families Citing this family (27)

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CN103656750B (en) * 2013-12-07 2014-12-24 西南交通大学 A method to improve multiple biomimetic functions on the surface of cardiovascular implant materials
CN103898055B (en) * 2014-01-20 2017-01-11 复旦大学附属中山医院 Establishment method of in-vitro cell culture platform with different matrix hardnesses
US10195313B2 (en) * 2014-04-10 2019-02-05 Wisconsin Alumni Research Foundation Method for forming hydrogel arrays using surfaces with differential wettability
JP6817194B2 (en) * 2014-05-12 2021-01-20 ルースタービオ インコーポレイテッド Instantly printable cells and integrated devices
US10808221B2 (en) 2014-12-22 2020-10-20 Corning Incorporated Proteinase-free coatings for colony passaging
EP3320081A1 (en) 2015-07-08 2018-05-16 Paris Sciences et Lettres - Quartier Latin Cell culture device
CN105294955B (en) * 2015-10-23 2018-04-24 华中科技大学 A kind of photocuring hydrogel broken up for many cells sorting and stem cell constituency and preparation method thereof
CN106609242B (en) * 2015-10-26 2020-01-21 泰陞国际科技股份有限公司 Cell bearing device and cell culture system
US11186810B2 (en) * 2016-03-16 2021-11-30 Shimadzu Corporation Cell culture vessel
MA44490A (en) * 2016-03-22 2019-01-30 Harvard College BIOCOMPATIBLE ADHESIVES AND THEIR METHODS OF USE
CN106754365B (en) * 2016-12-01 2019-06-14 天津市第一中心医院 A slow-release growth factor multi-cell spheroid culture membrane plate and its construction method
JP6721881B2 (en) * 2017-02-23 2020-07-15 日本電信電話株式会社 Cell scaffold material, cell model, and method for producing cell scaffold material
KR101989468B1 (en) * 2017-06-22 2019-06-14 성균관대학교 산학협력단 Preparing method of substrate for culturing stem cell
JPWO2019078278A1 (en) * 2017-10-18 2020-11-05 国立大学法人京都大学 Method for producing pluripotent stem cells for differentiating into cardiomyocytes
CN107764716B (en) * 2017-10-21 2020-08-14 云南中烟工业有限责任公司 A method for detecting the effect of cigarette smoke on cellular water permeability
CN107854218A (en) * 2017-12-12 2018-03-30 成都美益达医疗科技有限公司 A kind of disposable hydrocolloid antibacterial application
CN108078676A (en) * 2017-12-12 2018-05-29 成都美益达医疗科技有限公司 A kind of application for treating joint part wound
FR3081168B1 (en) * 2018-05-21 2022-03-11 Univ Bordeaux BIOREACTOR CELL CULTURE SYSTEM
CN108949524B (en) * 2018-07-26 2021-10-08 上海大学 Cage structure microporous culture dish for in vitro cell three-dimensional microstructure formation and preparation method thereof
CN109730811B (en) * 2018-12-17 2021-07-13 中国科学院上海硅酸盐研究所 Bone-imitation Haversian system bioactive scaffold and preparation method and application thereof
CN109628390A (en) * 2018-12-30 2019-04-16 深圳光彩生命工程技术有限公司 A kind of gelatinous cell culture formula of water-setting
JPWO2020203769A1 (en) * 2019-03-29 2020-10-08
CN111621468A (en) * 2020-05-21 2020-09-04 上海慧灯医疗科技有限公司 Method for preparing induced pluripotent stem cell-derived cardiomyocytes and fixing method and application thereof
CN113930337B (en) * 2020-06-29 2023-03-17 清华大学 Device for preparing cell clusters and construction method and application thereof
CN111748475A (en) * 2020-07-14 2020-10-09 兰州大学第一医院 A fluid cell culture device
JP7635934B2 (en) * 2021-03-03 2025-02-26 国立大学法人 東京大学 Evaluation equipment and evaluation method
CN113995444B (en) * 2021-11-03 2024-05-07 深圳大学 A hydrogel microneedle patch and its preparation method and application

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US20040259177A1 (en) * 2003-05-06 2004-12-23 Lowery Robert G. Three dimensional cell cultures in a microscale fluid handling system
US20060275270A1 (en) * 2004-04-28 2006-12-07 Warren William L In vitro mucosal tissue equivalent
US8906282B2 (en) * 2005-07-06 2014-12-09 Nanyang Technological University Micro-structured and nano-structured surfaces on biodegradable polymers
US8129188B2 (en) * 2005-09-29 2012-03-06 Industrial Technology Research Institute Cell culture apparatus and method of fabricating the apparatus
CN101007999B (en) * 2006-01-23 2012-04-25 杨炜 See-through three-dimensional cell culture system and its application in the culture of tissue cells and neonatal organs
JP4934360B2 (en) * 2006-07-05 2012-05-16 学校法人東京女子医科大学 Cell culture support
US20080220516A1 (en) * 2006-10-12 2008-09-11 Eddington David T Multi-well micropatterning by ablation
US8080418B2 (en) * 2007-03-09 2011-12-20 Corning Incorporated Method of making a three dimensional cell culture matrix
CA2702118A1 (en) * 2007-08-30 2009-03-12 President And Fellows Of Harvard College Compliant surface multi-well culture plate
CN101186706B (en) * 2007-11-15 2010-08-25 天津大学 Preparation method for PEG series gel nano particles
EP2420564A3 (en) * 2008-01-30 2013-12-25 Geron Corporation Synthetic surfaces for culturing cells in chemically defined media
WO2009103416A1 (en) * 2008-02-20 2009-08-27 Eth Zurich Multiwell culture plate for three-dimensional cultures
JP2010046012A (en) * 2008-08-21 2010-03-04 Konica Minolta Holdings Inc Substrate and method for cell culture
DE102009002577B4 (en) * 2009-04-22 2018-01-18 Leibniz-Institut Für Polymerforschung Dresden E.V. Cell culture carrier for the cultivation of human stem and progenitor cells and a method for cultivation

Also Published As

Publication number Publication date
EP2796541A1 (en) 2014-10-29
CN103146572A (en) 2013-06-12
JP2015501640A (en) 2015-01-19
US20140295553A1 (en) 2014-10-02
CN103146572B (en) 2016-01-06
WO2013083073A1 (en) 2013-06-13

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