CN103081701A - Scutellaria baicalensis sowing method - Google Patents

Scutellaria baicalensis sowing method Download PDF

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CN103081701A
CN103081701A CN2013100489136A CN201310048913A CN103081701A CN 103081701 A CN103081701 A CN 103081701A CN 2013100489136 A CN2013100489136 A CN 2013100489136A CN 201310048913 A CN201310048913 A CN 201310048913A CN 103081701 A CN103081701 A CN 103081701A
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root
flowered skullcap
seed
dilutions
millet
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CN103081701B (en
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张学文
宋国虎
徐亮
韩志斌
朱俊霖
吴凤文
李小丽
安鹏
宁翠玲
杨素君
曾燕
倪月
田东凤
杜越娟
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China National Corp. of Traclitional & Herbal Medicine, Chengde Medicinal Material Co., Ltd.
Chinese medicine company
Huamiao Chinese medicine Engineering Technology Development Center, Beijing
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China National Corp Of Traclitional & Herbal Medicine Chengde Medicinal Material Co Ltd
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Abstract

The invention relates to a scutellaria baicalensis sowing method. According to the scutellaria baicalensis sowing method, seeds of grain crops and scutellaria baicalensis are mixed for sowing ingeniously for the first time, the cultivation level of scutellaria baicalensis is improved, sowing is even, sowing quantity is small, soil fertility is improved, and especially the emergence rate of the scutellaria baicalensis is obviously improved. Compared with sowing methods with only scutellaria baicalensis seeds used or with sand-mixed scutellaria baicalensis seeds used, the emergence rate of the scutellaria baicalensis is improved by 20-45%, and therefore seedling preservation is benefited.

Description

A kind of root of large-flowered skullcap type of seeding
Technical field
The invention belongs to the Chinese medicine cultivation technical field, relate to the seeding technique of the root of large-flowered skullcap, relate to particularly a kind of root of large-flowered skullcap type of seeding that can significantly improve emergence rate.
Background technology
Baikal skullcap root is the dry root of the labiate root of large-flowered skullcap (Scutellaria baicalensis Georgi), have that heat-clearing and damp-drying drug, cool blood are antiabortive, the effect of detoxifcation, cure mainly the diseases such as warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, the swollen furuncle sore of carbuncle.Root of large-flowered skullcap main product in Hebei, the ground such as Liaoning, Shaanxi, Shandong, the Inner Mongol, Heilungkiang, wherein produced root of large-flowered skullcap curative effect as best take Chengde area, since ancient times by the good reputation titled with " the hot river root of large-flowered skullcap ".Existing root of large-flowered skullcap culture technique mostly is with planting seed breeding, but because root of large-flowered skullcap seed is less, very easily occurs the problems such as uneven sowing, emergence rate are low in seeding process, has had a strong impact on final emergence rate and the root yield of the root of large-flowered skullcap.
CN102349412A discloses a kind of cultivation method of the root of large-flowered skullcap, comprises selection of land, soil treatment, seed treatment, begins sowing in good time, field management, the extermination of disease and insect pest and gather.The root of large-flowered skullcap cultivation method of this invention, choosing suitable plantation plot, carrying out soil and seed treatment is the basis that guarantees high yield, quality better, suitable sowing time, in good time field management and timely the extermination of disease and insect pest promoted the growth of root of large-flowered skullcap target organ root, prevent the harm of disease, improved yield per unit area.
CN102823399A discloses a kind of cultivation method of the root of large-flowered skullcap, through selection of land whole ground, breeding, field management, the extermination of disease and insect pest, gather with process, the step such as storage.
CN102487702A discloses a kind of Yield-increasing plantation method for Chinese medicinal material scutellaria root, and it comprises: the wide row crop interplanting of (1) corn root of large-flowered skullcap method; (2) the narrow row crop such as soybean interplanting root of large-flowered skullcap method.The method of this invention can be impelled root of large-flowered skullcap large tracts of land, standardized planting, is a pattern that economic benefit is high of the nonirrigated farmland plantation root of large-flowered skullcap, can create considerable social and economic benefits and good ecological environment benefit.
CN102084771A discloses a kind of method for culturing baikal skullcap root drug, its inventive point is mainly kind, cuttage and seedling culture and cultivation management between the woods medicine, plant between the woods medicine is to reserve the root of large-flowered skullcap planting belt root of large-flowered skullcap of planting in the apricot woods, cuttage and seedling culture has proposed the measure of growing seedlings of plugged ear clip, chemicals treatment and plugged ear cuttage standard, and cultivation management brings forward scientific and reasonable fertilization mode, kind and consumption.This invention is carried out and is planted the solid growth method cultivation root of large-flowered skullcap between the woods medicine, can effectively utilize the natural resourcess such as soil, air, luminous energy, moisture and heat, and the woods medicine is two to be received.
Summary of the invention
The object of the invention is to provide the root of large-flowered skullcap type of seeding of sowing again after a kind of and cereal miscegenation, and the method is easy and simple to handle, sowing is even, and the emergence rate of the root of large-flowered skullcap significantly improves.
For achieving the above object, the present invention adopts following technical scheme:
A kind of root of large-flowered skullcap type of seeding comprises the steps:
(1) seed dressing: add insecticide and bactericide in root of large-flowered skullcap seed, stir, then dry.
Described root of large-flowered skullcap select seeds originates from the root of large-flowered skullcap seed in area, Chengde.
Described insecticide is selected from the combination of any one or at least two kinds in pyrethrins, rotenone, parathion, chlorophos, Rogor, sevin, Furadan, cypermethrin, sumicidin, decis, galecron, dimehypo, matrine; Be preferably cypermethrin and/or matrine; 150 of cypermethrin times of dilutions and/or be 150 times of dilutions of matrine more preferably.
described bactericide is selected from zineb (ethylene two (zinc dithiocarbamate)), Nong Side (sodium dichloro cyanurate), mancozeb (complex salt of the two two thiocarbamoyl manganese of ethylene and zinc), thiophanate-methyl (1, 2-two (3-carbometoxyl-2-ghiourea group) benzene), S-Ethyl ethylthio sulfonate (ethane thiosulfonic acid ethyl ester), tpn (termil), metalaxyl (D, L-N-(2, the 6-3,5-dimethylphenyl)-N-(2-methoxyl group acetyl) methyl lactamine), can kill, the combination of any one in carbendazim (N-(2-benzimidazole base)-methyl carbamate) or at least two kinds, be preferably Nong Side and/or S-Ethyl ethylthio sulfonate, 100 of the Nong Side of concentration 60g/L and/or S-Ethyl ethylthio sulfonate times of dilutions more preferably.
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 0.5-5:1 with cereal.
Described cereal crop such as millet, glutinous millet, broomcorn millet etc. all can be used in the present invention, can be the combination of any one or at least two kinds in them; Be preferably millet; " Shanxi Hong Gu " millet more preferably.
Described 0.5-5:1 can be for example 0.5-3:1,1.8-4.2:1,2.4-5:1,0.5:1,0.6:1,0.8:1,1:1,1.1:1,1.4:1,1.5:1,1.7:1,2:1,2.2:1,2.5:1,2.8:1,3:1,3.2:1,3.5:1,3.9:1,4:1,4.3:1,4.5:1,4.6:1,5:1; Be preferably 1-2:1; 1.5:1 more preferably.
(3) sowing: 2-6cm is dark for trench digging, drilling, and earthing 0.5-2cm is thick, compacting.
Described drilling refers to that the certain distance in interval broadcasts seed the type of seeding of strip equably, and be expert at and row between leave protuberance, the distance of preferred interval 40-50cm in the present invention.
Described 2-6cm can be for example 2-5.2cm, 3.7-4.5cm, 2.3-6cm, 2cm, 2.5cm, 2.65cm, 2.8cm, 3cm, 3.1cm, 3.5cm, 3.78cm, 4cm, 4.25cm, 4.4cm, 4.5cm, 4.9cm, 5cm, 5.2cm, 5.5cm, 5.6cm, 5.8cm, 6cm; Be preferably 3-5cm; 4cm more preferably.
Described 0.5-2cm can be for example 0.5-1.2cm, 0.7-1.5cm, 1.3-2cm, 0.5cm, 0.65cm, 0.8cm, 0.9cm, 1cm, 1.1cm, 1.25cm, 1.4cm, 1.6cm, 1.78cm, 1.9cm, 2cm; Be preferably 0.6-1.5cm; 1cm more preferably.
In sum, the preferred technical scheme of the present invention is:
A kind of root of large-flowered skullcap type of seeding comprises the steps:
(1) seed dressing: add insecticide cypermethrin and/or matrine and bactericide Nong Side and/or S-Ethyl ethylthio sulfonate in root of large-flowered skullcap seed, stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1-2:1 with millet;
(3) sowing: 3-5cm is dark for trench digging, drilling, and earthing 0.6-1.5cm is thick, compacting.
The further preferred technical scheme of the present invention is:
A kind of root of large-flowered skullcap type of seeding comprises the steps:
(1) seed dressing: add 150 times of dilutions of insecticide cypermethrin in the root of large-flowered skullcap seed and be 150 times of dilutions and the Nong Side of concentration of sterilant 60g/L and 100 times of dilutions of S-Ethyl ethylthio sulfonate of matrine, stirring, then drying;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1.5:1 with " Shanxi Hong Gu " millet;
(3) sowing: the trench digging 4cm dark, interval 40-50cm apart from drilling, earthing 1cm is thick, compacting.
The present invention adopts the type of seeding with cereal and the sowing of root of large-flowered skullcap seed mixing initiatively, improved the cultivation level of the root of large-flowered skullcap, sowing evenly, seeding quantity is few, soil fertility improves, particularly significantly improve the emergence rate of the root of large-flowered skullcap, improved 20%-45% than single root of large-flowered skullcap planting seed or the mixed husky insemination and emergence rate of root of large-flowered skullcap seed, more be conducive to save from damage seedling.
Description of drawings
Fig. 1 is the root of large-flowered skullcap quantity comparison diagram of emerging under different types of seeding in experimental example.
Below in conjunction with embodiment, the present invention is described in further detail.But following embodiment is only simple and easy example of the present invention, does not represent or limit the scope of the present invention, and protection scope of the present invention is as the criterion with claims.
Embodiment
For the present invention is described better, be convenient to understand technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
Embodiment 1:
A kind of root of large-flowered skullcap type of seeding, carry out (the soil fundamental property is as follows: pH value 6.7, organic matter 1.12%, full nitrogen 0.11%, full phosphorus 0.02%, potassium 1.71%, available nitrogen 23.4mg/kg, available phosphorus 1.75mg/kg, effective potassium 64.0mg/kg) in Tang Jia Zhuan Cun root of large-flowered skullcap base, Yu Erya town, Kuan Cheng county, Chengde, comprise the steps:
(1) seed dressing: 150 times of dilutions that add 150 times of dilutions of cypermethrin and matrine in the root of large-flowered skullcap seed (originating from Chengde area) as 100 times of dilutions of the Nong Side of insecticide and concentration 60g/L and S-Ethyl ethylthio sulfonate as bactericide, stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1:1 with " Shanxi Hong Gu " millet;
(3) sowing: the trench digging 3cm dark, interval 45cm apart from drilling, earthing 0.5cm, compacting.
Embodiment 2:
A kind of root of large-flowered skullcap type of seeding, carry out (the soil fundamental property is as follows: pH value 6.7, organic matter 1.12%, full nitrogen 0.11%, full phosphorus 0.02%, potassium 1.71%, available nitrogen 23.4mg/kg, available phosphorus 1.75mg/kg, effective potassium 64.0mg/kg) in Tang Jia Zhuan Cun root of large-flowered skullcap base, Yu Erya town, Kuan Cheng county, Chengde, comprise the steps:
(1) seed dressing: 150 times of dilutions that add 150 times of dilutions of cypermethrin and matrine in the root of large-flowered skullcap seed, stir as bactericide as 100 times of dilutions of the Nong Side of insecticide and concentration 60g/L and S-Ethyl ethylthio sulfonate, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1.5:1 with millet;
(3) sowing: the trench digging 4cm dark, interval 40cm apart from drilling, earthing 1cm, compacting.
Embodiment 3:
A kind of root of large-flowered skullcap type of seeding, (the soil fundamental property is as follows: pH value 6.9, organic matter 1.05%, full nitrogen 0.09%, full phosphorus 0.02%, potassium 1.01%, available nitrogen 19.7mg/kg, available phosphorus 6.34mg/kg, effective potassium 54.0mg/kg) carried out in root of large-flowered skullcap base, tile-roofed house village in the pure Gou Men of peace township, Luanping County, Chengde, comprises the steps:
(1) seed dressing: 150 times of dilutions that add 150 times of dilutions of cypermethrin and matrine in the root of large-flowered skullcap seed, stir as bactericide as 100 times of dilutions of the Nong Side of insecticide and concentration 60g/L and S-Ethyl ethylthio sulfonate, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1:1.5 with broomcorn millet;
(3) sowing: 4cm is dark for trench digging, drilling, and earthing 1cm is thick, compacting.
Embodiment 4:
A kind of root of large-flowered skullcap type of seeding, (the soil fundamental property is as follows: pH value 6.9, organic matter 1.05%, full nitrogen 0.09%, full phosphorus 0.02%, potassium 1.01%, available nitrogen 19.7mg/kg, available phosphorus 6.34mg/kg, effective potassium 54.0mg/kg) carried out in root of large-flowered skullcap base, tile-roofed house village in the pure Gou Men of peace township, Luanping County, Chengde, comprises the steps:
(1) seed dressing: add 150 times of dilutions of insecticide cypermethrin and 100 times of dilutions of bactericide S-Ethyl ethylthio sulfonate in the root of large-flowered skullcap seed (originating from Chengde area), stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 2:1 with glutinous millet;
(3) sowing: 6cm is dark for trench digging, drilling, earthing 2cm, compacting.
Embodiment 5:
A kind of root of large-flowered skullcap type of seeding, Weichang loess bank township, Chengde carries out in root of large-flowered skullcap base, loess bank village (the soil fundamental property is as follows: pH value 7.3, organic matter 0.98%, full nitrogen 0.08%, full phosphorus 0.03%, potassium 0.97%, available nitrogen 21.3mg/kg, available phosphorus 8.22mg/kg, effective potassium 45.0mg/kg), comprises the steps:
(1) seed dressing: add 150 times of dilutions of insecticide sumicidin and 150 times of dilutions of bactericide mancozeb in the root of large-flowered skullcap seed, stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1:2 with millet;
(3) sowing: the trench digging 5cm dark, interval 42cm apart from drilling, earthing 1.5cm is thick, compacting.
Embodiment 6:
A kind of root of large-flowered skullcap type of seeding, Weichang loess bank township, Chengde carries out in root of large-flowered skullcap base, loess bank village (the soil fundamental property is as follows: pH value 7.3, organic matter 0.98%, full nitrogen 0.08%, full phosphorus 0.03%, potassium 0.97%, available nitrogen 21.3mg/kg, available phosphorus 8.22mg/kg, effective potassium 45.0mg/kg), comprises the steps:
(1) seed dressing: add 150 times of dilutions of insecticide matrine and the Nong Side of concentration of sterilant 60g/L in the root of large-flowered skullcap seed, stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 5:1 with millet;
(3) sowing: the trench digging 2cm dark, interval 48cm apart from drilling, earthing 0.6cm is thick, compacting.
Experimental example:
Experiment is carried out in root of large-flowered skullcap base, tile-roofed house village in the pure Gou Men of peace township, Luanping County, Chengde, and the soil fundamental property is as follows: pH value 6.9, organic matter 1.05%, full nitrogen 0.09%, full phosphorus 0.02%, potassium 1.01%, available nitrogen 19.7mg/kg, available phosphorus 6.34mg/kg, effective potassium 54.0mg/kg.
Process one:
(1) seed dressing: 150 times of dilutions that add 150 times of dilutions of cypermethrin and matrine in the root of large-flowered skullcap seed, stir as bactericide as 100 times of dilutions of the Nong Side of insecticide and concentration 60g/L and S-Ethyl ethylthio sulfonate, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1:1.5 with millet;
(3) sowing: the trench digging 4cm dark, interval 45cm apart from drilling, earthing 1cm is thick, compacting.
Process two:
Step (1), (3) with process one identically, the step of step (2) miscegenation is: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1:1.5 with sand.
Process three:
Step (1), (3) with process one identically, do not contain the step of step (2) miscegenation, namely sow with true yellow a kind of reed mentioned in ancient books seed.
Three replicated plots are all established in above-mentioned three processing, and each replicated plot area is 2 * 3m, and the seeding quantity of each residential quarter root of large-flowered skullcap is 15.00g, the quantity statistics of emerging in the 3rd week in after planting.
During statistics, each replicated plot is chosen 5 points at random, and each dot-dash is got the area of 10 * 10cm, records respectively the quantity of emerging, and the average of 5 points is as the quantity of on average emerging of this residential quarter.
Result as shown in Figure 1, processing one the quantity of on average emerging is 47, processing two the quantity of on average emerging is 28, processing three the quantity of on average emerging is 38; Processing one has increased by 67.86% than the quantity of emerging of processing two, and processing one has increased by 23.68% than the quantity of emerging of processing three.As seen the of the present invention and type of seeding millet miscegenation has significantly improved emerge quantity and emergence rate.
Should be noted that and understand, in the situation that do not break away from the desired the spirit and scope of the present invention of accompanying claim, can make to the present invention of foregoing detailed description various modifications and improvement.Therefore, the scope of claimed technical scheme is not subjected to the restriction of given any specific exemplary teachings.
Applicant's statement, the present invention illustrates raw material proportioning of the present invention and preparation process by above-described embodiment, but the present invention is not limited to said ratio and preparation process, does not mean that namely the present invention must rely on above-mentioned preparation process and could implement.The person of ordinary skill in the field should understand, any improvement in the present invention is to the interpolation of the equivalence replacement of the selected raw material of the present invention and auxiliary element, the selection of concrete mode etc., within all dropping on protection scope of the present invention and open scope.

Claims (9)

1. a root of large-flowered skullcap type of seeding, is characterized in that, comprises the steps:
(1) seed dressing: add insecticide and bactericide in root of large-flowered skullcap seed, stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 0.5-5:1 with cereal;
(3) sowing: 2-6cm is dark for trench digging, drilling, and earthing 0.5-2cm is thick, compacting.
2. method according to claim 1, it is characterized in that, insecticide described in described step (1) is the combination of any one or at least two kinds in pyrethrins, rotenone, parathion, chlorophos, Rogor, sevin, Furadan, cypermethrin, sumicidin, decis, galecron, dimehypo, matrine; Be preferably cypermethrin and/or matrine; 150 of cypermethrin times of dilutions and/or be 150 times of dilutions of matrine more preferably.
3. method according to claim 1 and 2, it is characterized in that, bactericide described in described step (1) is zineb (ethylene two (zinc dithiocarbamate)), Nong Side (sodium dichloro cyanurate), mancozeb (complex salt of the two two thiocarbamoyl manganese of ethylene and zinc), thiophanate-methyl (1, 2-two (3-carbometoxyl-2-ghiourea group) benzene), S-Ethyl ethylthio sulfonate (ethane thiosulfonic acid ethyl ester), tpn (termil), metalaxyl (D, L-N-(2, the 6-3,5-dimethylphenyl)-N-(2-methoxyl group acetyl) methyl lactamine), can kill, the combination of any one in carbendazim (N-(2-benzimidazole base)-methyl carbamate) or at least two kinds, be preferably Nong Side and/or S-Ethyl ethylthio sulfonate, 100 of the Nong Side of concentration 60g/L and/or S-Ethyl ethylthio sulfonate times of dilutions more preferably.
4. one of according to claim 1-3 described methods, is characterized in that, cereal described in described step (2) are the combination of any one or at least two kinds in millet, glutinous millet, broomcorn millet; Be preferably millet; " Shanxi Hong Gu " millet more preferably.
5. one of according to claim 1-4 described methods, is characterized in that, described in described step (2), volume ratio is 1-2:1; Be preferably 1.5:1.
6. one of according to claim 1-5 described methods, is characterized in that, described in described step (3), trench digging is dark for 3-5cm; Be preferably 4cm dark.
7. one of according to claim 1-6 described methods, is characterized in that, described in described step (3), earthing is that 0.6-1.5cm is thick; Be preferably 1cm thick.
8. one of according to claim 1-7 described methods, is characterized in that, described method comprises the steps:
(1) seed dressing: add insecticide cypermethrin and/or matrine and bactericide Nong Side and/or S-Ethyl ethylthio sulfonate in root of large-flowered skullcap seed, stir, then dry;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1-2:1 with millet;
(3) sowing: 3-5cm is dark for trench digging, drilling, earthing 0.6-1.5cm, compacting.
9. one of according to claim 1-8 described methods, is characterized in that, described method comprises the steps:
(1) seed dressing: add 150 times of dilutions of insecticide cypermethrin in the root of large-flowered skullcap seed and be 150 times of dilutions and the Nong Side of concentration of sterilant 60g/L and 100 times of dilutions of S-Ethyl ethylthio sulfonate of matrine, stirring, then drying;
(2) miscegenation: the root of large-flowered skullcap seed after step (1) is dried evenly mixes according to the volume ratio of 1.5:1 with " Shanxi Hong Gu " millet;
(3) sowing: the trench digging 4cm dark, interval 40-50cm apart from drilling, earthing 1cm, compacting.
CN201310048913.6A 2013-02-06 2013-02-06 Scutellaria baicalensis sowing method Active CN103081701B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103404344A (en) * 2013-08-09 2013-11-27 中国药材集团承德药材有限责任公司 Method for improving yield and quality of scutellaria baicalensis seeds
CN103975786A (en) * 2014-05-30 2014-08-13 孟祥才 Method for improving quality of radix scutellariae with sodium hyposulfite
CN105191626A (en) * 2015-09-24 2015-12-30 太仓市南杨农场专业合作社 Scutellaria baicalensis high-yield culture technology
CN105993495A (en) * 2016-05-29 2016-10-12 寿光市新世纪种苗有限公司 Biological trapping method for controlling tomato root-knot nematode

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
无: "黄芪的播种", 《瓜果蔬菜报》 *
王增年等: "《农业新技术一百条》", 31 May 1963, 河北人民出版社 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103404344A (en) * 2013-08-09 2013-11-27 中国药材集团承德药材有限责任公司 Method for improving yield and quality of scutellaria baicalensis seeds
CN103975786A (en) * 2014-05-30 2014-08-13 孟祥才 Method for improving quality of radix scutellariae with sodium hyposulfite
CN105191626A (en) * 2015-09-24 2015-12-30 太仓市南杨农场专业合作社 Scutellaria baicalensis high-yield culture technology
CN105993495A (en) * 2016-05-29 2016-10-12 寿光市新世纪种苗有限公司 Biological trapping method for controlling tomato root-knot nematode

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