CN103055014A - Folium ginkgo health product for preventing senile dementia and preparation method thereof - Google Patents

Abstract

The invention provides a folium ginkgo health product for preventing senile dementia, which comprises raw materials by weight portions: 10-30 portions of folium ginkgo, 10-20 portions of polygala tenuifolia, 10-20 portions of prepared rehmannia root, 10-20 portions of Chinese angelica, 10-20 portions of dogwood, 10-20 portions of root of red-rooted salvia, 10-20 portions of poria with hostwood, 10-20 portions of walnut kernel, 10-20 portions of codonopsis pilosula, 10-20 portions of platycladi seed, 10-20 portions of herba epimedii, 10-20 portions of astragalus mongholicus, 10-20 portions of polygonum multiflorum, 10-20 portions of white paeony root, 10-20 portions of fructus ligustri lucidi, 10-20 portions of bunge cherry seed, 10-20 portions of albizia flower and 10-20 portions of tuber fleeceflower stem. The folium ginkgo is extracted, the effective ingredients of the folium ginkgo are retained, the toxicity is reduced, and medicine directly reaches lesions to regulate qi and blood and balance yin and yang. On the one hand, the brain cell shrinkage function is improved, the conditions of Qi-stagnation and blood stasis and venation with the blood stasis are improved, qi and blood keep unblocked, and the blood oxygen supply of brain tissue is secured; and on the other hand, the damaged brain cell tissue is repaired and activated, and the normal brain function is restored.

Description

A kind of ginkgo Leaves health care product that prevent senile dementia and preparation method thereof

Technical field

The present invention relates to contain the intelligence benefiting health article that derives from plant composition, particularly a kind of ginkgo Leaves health care product that prevent senile dementia and preparation method thereof.

Background technology

Alzheimer (Alzheimer disease, AD) is again alzheimer disease, is that a kind of central nervous system degeneration is sick, insidious onset, and the course of disease is chronic progressive external, is modal one type of senile dementia.Main manifestations is the neuropsychic symptoms such as gradual dysmnesia, cognitive dysfunction, personality changes and aphasis, has a strong impact on social activity, occupation and vital function.The cause of disease and the pathogenesis of AD are not yet illustrated, and the characteristic pathological change is the extracellular senile plaque of amyloid beta formation of deposits and the interior neurofibrillary tangles of neurocyte of Protein tau Hyperphosphorylationof formation, and neuron loss companion glial cells hyperplasia etc.

Typical first sign is dysmnesia, and in early days take nearly Impairment of Memory as main, Impairment of Memory far away is relatively light, shows as thing to firm generation, just what someone said can not be remembered, and forgets familiar name, and relatively clear to thing memory of the remote past.Often be left in the basket in early days, be considered to the old people and like to forget things, but can affect patient's daily life gradually.Simultaneously language function is impaired gradually, the phenomenon of looking for word, looking for the name difficulty occurs, dyscalculia, time disturbance of orientation to place can occur, carry out function reduction etc.

Folium Ginkgo is a kind of plant with very high medical value, and ginkgo is one of ancient seeds of China, and it is the tree of the medical treatment of mystery, Jurassic Period dinosaur control terrestrial time before more than 200,000,000 5 thousand year, and Semen Ginkgo is one of the most flouring plant.Life on earth is gone through the change in 100000000000 years, especially after the glacier covering of the 4th century, only has Semen Ginkgo still to keep its most original looks, is called as " living fossil " in the organic evolution history.Its leaf, fruit, seed have higher medical value, and its pharmacological action constantly is familiar with, and clinical application range progressively enlarges.Ginkgo has another name called maidenhair tree, is the rarity of China, and is rare valuable, is described as " hollywood lignumvitae ", " living fossil " of the earth.。

Contain the multiple components useful with health such as natural activity flavone and amarolide in the Folium Ginkgo, has dissolving cholesterol, the effect of blood vessel dilating has positive effect to improving disordered brain function, arteriosclerosis, hypertension, dizzy, tinnitus, headache, senile dementia, hypomnesis etc.Its diseases prevention, cure the disease, the value of body-building is early on the books in the Compendium of Material Medica of Ming Dynasty's Li Shizhen (1518-1593 A.D.).Detect proof through national health department: drink Folium Ginkgo tea and can obviously reduce serum cholesterol, triglyceride and low-density blood lipoprotein, reduce the effect of depot lipid in the body.For fat-reducing of the complementary controls of diseases of cardiovascular and cerebrovascular systems patient such as adjusting, hypertension and the coronary heart disease of hyperlipidemia and obese type crowd etc. good effect being arranged, is the desirable beverage of prophylactic treatment senile dementia.It strengthens limbs peripheral nerve function, and hypermnesis prevents and improves senile dementia and apoplexy, applicable dizziness and tinnitus, hand and foot numbness or ice-cold person, hypomnesis or concentrator not, apoplexy person; It is the optimal pharmaceutical compositions of cardiovascular and cerebrovascular disease of controlling, prevent recognized in the world.Though Folium Ginkgo energy blood circulation promoting, the prevention cardiovascular diseases, but can not take simultaneously other and control cardiovascular drugs (for example aspirin), Semen Ginkgo extrac is the concentrated granular shape, have very strong removing free radical and antioxidation, the synthetic collagen Proteins of yellow glycoside, aminoacid and aminoacid in the Folium Ginkgo is improved looks to human body, and the check melanin growth keeps skin gloss and elasticity to play no small effect.In addition, according to modern clinical research, the chronic schizophrenia take negative symptoms as main (such as social withdrawal, sluggishness, the poverty of thought, the symptom such as dull) there is remarkable result, cerebellar atrophy (senile dementia) is also had certain effect.

Although the ginkgo Leaves health care effect is very strong, Folium Ginkgo contains poisonous composition, and brewing up with it to cause clonospasm, neural paralysis, platycoria, allergy and other side effect.Folium Ginkgo can not together brew up with Folium Camelliae sinensis and Flos Chrysanthemi.Anemia of pregnant woman and child, old man are careful.A kind of composition is arranged ginkgoic acid in the Folium Ginkgo, if the dosage of usefulness is excessive, perhaps Time of Administration is long, can damage heart.Semen Ginkgo contains a large amount of ginkgoic acids, ginkgoic acid be contain virose, the leaf of generally buying does not pass through deep processing and extraction, the ginkgoic acid content of the inside is up to 3000-4000ppm, special ginkgoic acid is water miscible, one soaked Semen Ginkgo pyrite and the active substance such as Semen Ginkgo lactone of not only not having the inside, effect is opposite on the contrary, the inside is poison entirely.

Although the ginkgo Leaves health care effect is very strong, Folium Ginkgo contains poisonous composition, and brewing up with it to cause clonospasm, neural paralysis, platycoria, allergy and other side effect.Ginkgoic acid, improper use or dosage are too large, are easy to cause poisoning.So at present in the urgent need to a kind of technique, health-care effect that can the application Folium Ginkgo makes again its poisonous material drop to minimum.Anemia of pregnant woman and child, old man are careful.The main component flavone compound of Folium Ginkgo is a kind of powerful platelet activating factor inhibitor, and long-term taking may suppress hematoblastic cohesion function, and the danger of corresponding increase cerebral hemorrhage.Therefore, the old people will stop before going too far when taking Folium Ginkgo, and notes regularly detecting the blood coagulation situation, not the blindness long-term taking.The patient who becomes fragile for existing senile sclerosis of blood vessels, blood vessel more can not long-term taking, in order to avoid cause cerebral hemorrhage.A kind of composition is arranged ginkgoic acid in the Folium Ginkgo, if the dosage of usefulness is excessive, perhaps Time of Administration is long, can damage heart.

Summary of the invention

There are a lot of defectives in prior art, and China progresses into aging society, and the annual a large amount of manpower and materials of cost that need of society solve this social contradiction.And Folium Ginkgo extract can strengthen limbs peripheral nerve function, and hypermnesis prevents and improves senile dementia and apoplexy, applicable dizziness and tinnitus, hand and foot numbness or ice-cold person, hypomnesis or concentrator not, apoplexy person; It is the optimal pharmaceutical compositions of cardiovascular and cerebrovascular disease of controlling, prevent recognized in the world.But Folium Ginkgo contains poisonous composition, and brewing up with it to cause clonospasm, neural paralysis, platycoria, allergy and other side effect; A kind of composition is arranged ginkgoic acid in the Folium Ginkgo, if the dosage of usefulness is excessive, perhaps Time of Administration is long, can damage heart.The present invention extracts Folium Ginkgo, not only kept its beneficiating ingredient, reduce toxicity, add some blood circulation promoting and blood stasis dispelling, the Chinese crude drug of hypermnesis effect can improve microcirculation, strengthen limbs peripheral nerve function, hypermnesis makes the people be easy to accept, and more can reach preferably the effect that the prevention senile dementia occurs.

For solving above-mentioned technical barrier, the invention provides a kind of ginkgo Leaves health care product that prevent senile dementia, the parts by weight of various raw materials can be 10～30 parts of Folium Ginkgos, 10～20 parts of Radix Polygalaes in the described prevention senile dementia ginkgo Leaves health care product, 10～20 parts in Radix Rehmanniae Preparata, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Fructus Corni, 10～20 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Poria cum Radix Pinis, 10～20 parts of Semen Juglandis, 10～20 parts of Radix Codonopsis, 10～20 parts of Semen Platycladi, 10～20 parts of Herba Epimedii, 10～20 parts of the Radixs Astragali, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Semen Prunis, 10～20 parts of Flos Albiziaes, 10～20 parts of Caulis Polygoni Multiflori.

The ginkgo Leaves health care product of prevention senile dementia, the parts by weight of various raw materials can be preferably in its described prevention senile dementia ginkgo Leaves health care product: 10～30 parts of Folium Ginkgos, 10～15 parts of Radix Polygalaes, 10～15 parts in Radix Rehmanniae Preparata, 10～15 parts of Radix Angelicae Sinensis, 10～15 parts of Fructus Corni, 10～15 parts of Radix Salviae Miltiorrhizaes, 10～15 parts of Poria cum Radix Pinis, 10～15 parts of Semen Juglandis, 10～15 parts of Radix Codonopsis, 10～20 parts of Semen Platycladi, 10～20 parts of Herba Epimedii, 10～20 parts of the Radixs Astragali, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Semen Prunis, 10～20 parts of Flos Albiziaes, 10～20 parts of Caulis Polygoni Multiflori.

The ginkgo Leaves health care product of prevention senile dementia, the parts by weight of various raw materials can also be preferably in its described prevention senile dementia ginkgo Leaves health care product: 10～30 parts of Folium Ginkgos, 10～20 parts of Radix Polygalaes, 10～20 parts in Radix Rehmanniae Preparata, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Fructus Corni, 10～20 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Poria cum Radix Pinis, 10～20 parts of Semen Juglandis, 10～20 parts of Radix Codonopsis, 10～15 parts of Semen Platycladi, 10～15 parts of Herba Epimedii, 10～15 parts of the Radixs Astragali, 10～15 parts of Radix Polygoni Multiflori Preparata, 10～15 parts of the Radix Paeoniae Albas, 10～15 parts of Fructus Ligustri Lucidi, 10～15 parts of Semen Prunis, 10～15 parts of Flos Albiziaes, 10～15 parts of Caulis Polygoni Multiflori.

The ginkgo Leaves health care product of prevention senile dementia, the dosage form of its described prevention senile dementia ginkgo Leaves health care product can be oral solutions, unguentum, tablet, pill or powder.

For solving the problems of the technologies described above, the present invention also provides a kind of preparation method of preventing the ginkgo Leaves health care product of senile dementia, when the preparation method of described ginkgo Leaves health care product may further comprise the steps:

A. the Folium Ginkgo preparation is extracted;

B. the sucking filtration of Folium Ginkgo extract;

C. Folium Ginkgo extract is condensed into particulate matter, powder or unguentum;

D. with the agent of surplus stock component granulation, powder or unguentum.

E. pack after granule obtained above, powder or unguentum being mixed with granule, powder or the unguentum of the Folium Ginkgo extract of making.

Among the described step a, can get fresh Folium Ginkgo, clean, dry, in 70 ℃ of baking ovens, baking 8-12h, taking-up places cooling in the drying baker, pulverizes the 80-100 mesh sieve with pulverizer, and powder places 95% ethanol to soak 1-2 hour, in water-bath, be heated to 60 ℃～70 ℃, lixiviate 2h, every 10min stirs once, use filtered through gauze, add 60% ethanol in the residue, 60 ℃～70 ℃ are continued lixiviate 2h, every 10min stirs once, and filtered through gauze merges lixiviating solution.

Among the described step b, ginkgo biloba succi can be used first the gauze coarse filtration, remove the bulky grain precipitation, to improve sucking filtration speed; The sucking filtration instrument is installed, and adding a small amount of lixiviating solution in the buchner funnel carries out sucking filtration, after the sucking filtration end, gets filtrate, abandons filtering residue.

Among the described step c, the Folium Ginkgo slag that ethanol extraction can be crossed is put into 10 times of water gagings, heating and refluxing extraction 1 time, extraction time is 1～2 hour, the 100-120 order filters, be the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams again, 1.38 filtrate when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃; Merge twice filtrate heating and be concentrated into the extractum shape.

In the described steps d, the surplus stock component can be put into 10 times of amount ethanol, heating and refluxing extraction 2 times each 1～2 hour, leaves standstill 2 extracting solution merging; The medicinal residues of the component raw medicinal material of more above-mentioned ethanol extraction being crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution merging are left standstill; Above-mentioned two kinds of extracting solution are merged, 1.36 o'clock filtrate when the concentrating under reduced pressure relative density is 80 ℃, Recycled ethanol is adjusted proportion to 1.06 with gained filtrate, and extractum reduces pressure to get.

Among the described step e, can merge making the Folium Ginkgo extract extractum that extractum and above-mentioned steps make in the step 9, fully mix granulation agent, powder, pill, tablet or unguentum

The invention solves the virose shortcoming of Folium Ginkgo; And the beneficiating ingredient that has kept greatly Folium Ginkgo makes it not have work in-process to run off, and has on the make avoided secondary pollution, is the health product of green health environmental protection fully.Folium Ginkgo extract can strengthen limbs peripheral nerve function, and hypermnesis prevents and improves senile dementia and apoplexy, applicable dizziness and tinnitus, hand and foot numbness or ice-cold person, hypomnesis or concentrator not, apoplexy person; It is the optimal pharmaceutical compositions of cardiovascular and cerebrovascular disease of controlling, prevent recognized in the world.The present invention extracts Folium Ginkgo, has not only kept its beneficiating ingredient, reduces toxicity, add some blood circulation promoting and blood stasis dispelling, the Chinese crude drug of hypermnesis effect can improve microcirculation, strengthen limbs peripheral nerve function, hypermnesis, the medicine focus of going directly, QI and blood regulating, Yin Yang balancing, strengthen on the one hand the brain cell contractile function, improve the situation of qi depression to blood stasis, venation block, make QI and blood keep unimpeded, ensure the blood oxygen of brain supply; Repair on the other hand and activate impaired brain cell tissue, recover the brain normal function, make the people be easy to accept, more can reach preferably the effect that the prevention senile dementia occurs.

The specific embodiment

AD is generally in presenium and geratic period onset, and insidious onset is difficult for being found in early days, and the state of an illness is made progress gradually.Core symptom is ABC three parts, that is: activity of daily living reduces (Activities of dailyliving), mental act unusual (Behavior), cognitive competence decline (Cognition).The artificial clinical course with AD roughly is divided into three phases clinically.Phase I (1-3): be the mild dementia phase.Show as hypomnesis, outstanding to recent amnesia; Judgement descends, and patient can not analyze, think deeply, judge event, is difficult to process complicated problem; Work or housework are careless, can not independently do shopping, economic affairs etc. social difficulty; Although still can do a little routine works of being familiar with, new things is shown ignorantly be difficult to resolve, apathy, excitation often has suspicious once in a while; The time of occurrence disorientation can be made orientation to residing place and personage, to the directed difficulty in geographical position of living in, the visual space ability of labyrinth; Speech vocabulary is few, the name difficulty.Second stage (2-10): be the moderate dementia phase.Show as far and near memory and be badly damaged, the visual space ability of simple structure descends time, disturbance of orientation to place; Aspect processing problem, the similitude of distinguishing things and the discrepancy grievous injury is being arranged; Can not independently carry out outdoor activity, wear the clothes, Personal hygiene and keep wanting help aspect the personal appearance; Calculating can not; Various nervous symptoms appear, visible aphasia, apraxia and agnosia; Emotion is often walked about and is not stopped, visible urinary incontinence by the indifferent irritability uneasiness that becomes.Phase III (8-12): be the severe dementia phase.The memory of fragment is only deposited in serious memory loss; Daily life can not be taken care of oneself, gatism, present keep silent, limbs are stiff, the visible pyramidal sign of having a medical check-up is positive, and Primitive reflexs such as holding, grope and suck is arranged by force.Final stupor is generally died from the complication such as infection

The development and use of Folium Ginkgo are from the thirties in 20th century, and Japan and West Germany have namely begun the research of Folium Ginkgo.Along with deepening continuously of modern pharmacological research, to find not only to contain Flavonoid substances in the Folium Ginkgo, and also have the chemical compounds such as terpene lactones, phenols, the pharmacological action of their brilliances is familiar with gradually by people.The Mineral Concentrations such as VC, VE, carotene and calcium, phosphorus, boron, selenium are also very abundant in the Folium Ginkgo, surpass general fruit and vegetable and edible plant raw material.Biological anti-oxidant is a class material that directly and indirectly has anti-oxidation function in the body.In Folium Ginkgo, contain two class antioxidants--nutrient antioxidant and non-nutritive antioxidant.The former mainly contains carotene, VC, VE, selenium, zinc, copper etc.; the latter mainly contains ginkgetin, terpene lactones, catechin, Polyphenols etc.; content is also very abundant, and they are not subjected to have ten important effects aspect the oxidative damage due to the free radical at the protection body.Therefore, Folium Ginkgo integrates nutrition, health care, treatment function, and Folium Ginkgo is developed as the resource that high nutrition, health care are worth, and this is significant for improving the Folium Ginkgo comprehensive utilization ratio.

Clinical observation proves that Folium Ginkgo has following several effect: 1, reduce cholesterol levels in the blood of human body, prevent arteriosclerosis.To be short of physical strength behind the middle-aged and elderly people light activity, palpitating speed, chest pain, the effect of being significantly improved such as feel dizzy.2, reduce blood pressure by increasing the saturating property of blood vessel and elasticity, preferably antihypertensive effect is arranged.3, the deposition composition on the elimination blood vessel wall improves blood flow and changes, and promotes erythrocyte deformability, and blood viscosity lowering makes blood flow unobstructed, can prevent and treat cerebral hemorrhage and cerebral infarction.The senile dementia that arteriosclerosis is caused also has certain curative effect.Gingko leaf preparation and antidiabetic drug share the treatment diabetes good therapeutic effect, can be used for the adjuvant of diabetes.5, Folium Ginkgo extract can strengthen limbs peripheral nerve function, and hypermnesis prevents and improves senile dementia and apoplexy, applicable dizziness and tinnitus, hand and foot numbness or ice-cold person, hypomnesis or concentrator not, apoplexy person; It is the optimal pharmaceutical compositions of cardiovascular and cerebrovascular disease of controlling, prevent recognized in the world.6, reduce level of lipid, reduce freckle, moist skin, beautiful appearance.

But anemia of pregnant woman of the present invention and child, old man are careful.The main component flavone compound of Folium Ginkgo is a kind of powerful platelet activating factor inhibitor, and long-term taking may suppress hematoblastic cohesion function, and the danger of corresponding increase cerebral hemorrhage.Therefore, the old people notes regularly detecting the blood coagulation situation taking Folium Ginkgo, not long-term taking blindly.The patient who becomes fragile for existing senile sclerosis of blood vessels, blood vessel more can not long-term taking, in order to avoid cause cerebral hemorrhage.A kind of composition is arranged ginkgoic acid in the Folium Ginkgo, if the dosage of usefulness is excessive, perhaps Time of Administration is long, can damage heart.

The flat sweet in the mouth of Semen Platycladi, the heart, kidney, large intestine channel.The effect of tool tranquilizing by nourishing the heart, loosening bowel to relieve constipation.Control the diseases such as palpitation with fear, insomnia, seminal emission, night sweat, constipation, energy tranquilizing by nourishing the heart, loosening bowel to relieve constipation.Be used for restlessness of asrhenia type and insomnia, severe palpitation, dryness of the intestine makes the secret disease that Dengs." medicineization justice ": Semen Platycladi, the saturating heart of fragrance, the body profit is grown blood.Same Poria cum Radix Pini, Semen Ziziphi Spinosae, the Radix Rehmanniae, Radix Ophiopogonis are product clearly in turbid, and it is timid to cure mainly mind void, the palpitation with fear severe palpitation, and color is wan and sallow, the skin pruritus due to dryness, the merit of the blood that all nourishes heart is also.It is all dense to get again abnormal smells from the patient, returns kidney in turbid, and same Radix Rehmanniae Preparata, Carapax et Plastrum Testudinis, Fructus Lycii, Radix Achyranthis Bidentatae for packing bone marrow, cure mainly damage of kidney-YIN, the back severe disease, and sufficient knee joint is weak, night sweat due to yin deficiency, all the dry power of nourishing kidney is also.Sweet in the mouth also can delay liver, and the deficiency of tonifying liver gallbladder is extremely reliable, but the flat power of property is slow, should be multiplex be wonderful.

Radix Polygalae is bitter, hot, tepor.GUIXIN, kidney, lung meridian.Function cures mainly the Fructus Alpiniae Oxyphyllae of calming the nerves, and eliminates the phlegm detumescence.Be used for the insomnia and dreamful sleep that disarmony between the heart and kidney causes, forgetful palpitation with fear, staring spells, expectoration is not well, sore swollen toxin, breast swell and pain.Be applied to insomnia and dreamful sleep, severe palpitation, forgetful.This product toil is warm in nature, property is kind lead off sensible, happily gas and mind tranquilizing and the heart calming, can lead to kidney qi and strong will is not forgotten, be the by force good merchantable brand of knowledge of restoring normal coordination between the heart and kidney, stable mind, Fructus Alpiniae Oxyphyllae.Cure mainly the diseases such as malaise, insomnia, palpitation with fear of disarmony between the heart and kidney, normal and the same usefulness of medicinal for tranquilizing mind such as Poria cum Radix Pini, Dens Draconis, Cinnabaris are such as Radix Polygalae ball (" the Zhang Shi doctor is led to "); Control forgetful card, normal and Radix Ginseng, Poria, the same usefulness of Rhizoma Acori Graminei such as Kaixin San (" prescriptions worth thousand gold "), if Fang Zhongzai adds Poria cum Radix Pini, are not namely forgotten fall apart (" Standards of Diagnosis and Treatment ")." book on Chinese herbal medicine is just ": Radix Polygalae, the wholwe-hearted kidney of merit, so but the relieving palpitation spasmolytic, the peace that exorcises evil spirits dream, Yang strengthening and life essence tonifying, strong will power-assisted.With its gas lift, so same Radix Ginseng, Radix Glycyrrhizae, Semen Ziziphi Spinosae, the utmost point can be lifted and fall into consolidating semen, handing-over extreme misery.

Fructus Corni cures mainly pathogen cold and heat under the heart, and warming middle-JIAO by cold warm numbness, goes three worms, for a long time made light of one's life by commiting suicide reinforcing YIN-essence benefit essence, settling five organs, logical nine orifices, the merit of dribbling urination only of clothes; For a long time clothes make eye bright, brute force all the year round.Fructus Corni has another name called Fructus Corni, medicine Fructus Jujubae, real Fructus Jujubae, Fructus Corni, meat Fructus Jujubae etc., is China's rare Chinese medicine commonly used, and applicating history is long.It is gentle with its benefit power, tonifying YANG and do not help fire, YIN nourishing and oiliness diaphragm, convergence and do not stay the evil special efficacy that waits by successive dynasties usefulness that medical science is liked.Zhang Zhongjing has been formulated " JINGUI SHENQI WAN " take Fructus Corni as monarch.According to chemical analysis, Fructus Corni contains the effective ingredient such as the stronger cornin of physiologically active, tartaric acid, gallic acid, malic acid, resin, tannin and multivitamin, having and strengthen immunity, antiinflammatory, the pharmacological action such as antibiotic, is the Herba indigoferae Pseudotinctoriae of commonly using in the tcm clinical practice.

Fructus Ligustri Lucidi is used for the poor vision of the hepatic and renal YIN deficiency, visual deterioration, early whitening of beard and hair, soreness of waist tinnitus and fever due to yin deficiency etc.This product energy tonifying liver kidney yin, but the efficacy of a drug is gentle, must slowly get effect.Control poor vision, often join Radix Rehmanniae Preparata, Fructus Lycii is equal to usefulness, controls early whitening of beard and hair, often joins the same usefulness of Fructus Mori, and Cortex Lycii is often joined in fever due to yin deficiency.The hepatic and renal YIN deficiency, Light-headedness, seminal emission tinnitus, soreness of the waist and knees, early whitening of beard and hair, coronary heart disease, hyperlipemia, hypertension, chronic hepatitis.Protective effect research to liver is thought, Fructus Ligustri Lucidi contains oleanolic acid, give rat skin lower injection, can reduce serum glutamic pyruvic transminase, hepatic injury to Carbon Tetrachloride Induced has obvious protective effect, and can promote liver cell regeneration. and oleanolic acid also can make in the liver triglyceride accumulate that minimizings, hepatocellular degeneration necrosis obviously alleviate, glycogen is accumulated increase, serum γglobulin decline; Mitochondrial swelling and the degeneration of endoplasmic reticulum vesicle alleviates, the matter inflammatory reaction weakens between hepatic tissue in the visible hepatocyte of ultrastructure.

Radix Codonopsis is China's traditional tonic commonly used, and the Radix Codonopsis that produce take area, Shangdang, Shanxi ancient times has invigorating the spleen and replenishing QI, the effect of spleen invigorating lung benefiting as top grade.Modern study, Radix Codonopsis contain various saccharides, phenols, sterol, volatile oil, scutellarein glucosid, Saponin and Alkaloid, have enhancing immunity, blood vessel dilating, blood pressure lowering, improve microcirculation, strengthen the effects such as hemopoietic function.The leukopenia that in addition chemotherapy radiotherapy is caused has castering action.Radix Codonopsis has invigorating the spleen and replenishing QI, the effect of spleen invigorating lung benefiting.Be used for deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, the dyspnea due to deficiency cough, interior-heat is quenched one's thirst etc." Bencao Congxin " record: " invigorating the spleen and replenishing QI and taste, relieving thirst and restlessness.A little less than the middle feeble QI, mend in order to transfer, very smooth and proper.”

The Chinese medicine Radix Polygoni Multiflori have Radix Polygoni Multiflori and Radix Polygoni Multiflori Preparata minute, direct slicing is used as medicine and is Radix Polygoni Multiflori, mixes to dry to be used as medicine after the steaming with the Semen sojae atricolor liquor to be Radix Polygoni Multiflori Preparata.The function of the two is different: the detoxifcation of Radix Polygoni Multiflori function, eliminating carbuncle, loosening bowel to relieve constipation are usually used in treating scrofula carbuncle sore, rubella pruritus, dryness of the intestine constipation; The invigorating the liver and kidney of Radix Polygoni Multiflori Preparata function, benefiting essence-blood, black beard and hair, bone and muscle strengthening are used for blood deficiency and yellow complexion, vertigo and tinnitus, early whitening of beard and hair, soreness of the waist and knees, numb limbs and tense tendons, bleeding not during menses, chronic malaria body void etc.Radix Polygoni Multiflori Preparata of the present invention is the good merchantable brand of invigrating kidney.

The Cortex Albiziae nature and flavor are sweet, flat.The merit that resolving depression and blood, mind calming, subduing inflammation are arranged.The effect that the irritability of controlling, melancholy, insomnia, lung abscess, carbuncle, scrofula, muscle fracture wound are arranged." book on Chinese herbal medicine is looked for the truth ": " Cortex Albiziae.Why Herba Albiziae is named, and the internal organs peace of its clothes is supported, and makes us joyous happy, so with joyous name." herbal classic ": main settling five organs, and will, make us happy carefree.Be usually used in the unsuccessful melancholy of feelings will and induced insomnia person, malaise etc. are lower, clinically manyly join with Semen Platycladi, the heart, Semen Ziziphi Spinosae is equal to usefulness, with enhancing nourish heart dissipating depression of QI, tranquilizing the mind effect.

The Semen Juglandis nature and flavor are sweet, temperature.Return kidney, lung, large intestine channel.The kidney invigorating, warming the lung, intestine moistening.Be used for soreness of the waist and knees, impotence and seminal emission, deficiency and coldness is breathed heavily and is coughed, constipation.Brain-strengthening is anti-old: Semen Juglandis contains more protein and the essential unsaturated fatty acid of human nutrition, and these compositions are all the important substance of cerebral tissue cellular metabolism, can nourish brain cell, cerebral function improvement.

Poria cum Radix Pini's sweet in the mouth, light, property are flat, be used as medicine have promoting diuresis to eliminate damp pathogen, the function of strengthening the spleen stomach function regulating, mind tranquilizing and the heart calming.Nature and flavor are sweet, light flat.The functions such as eliminating dampness by diuresis, spleen invigorating, mind calming are arranged.Be used for phlegm retention, edema, dysuria, have loose bowels, cardiopalmus, dizzy.Cortex Sclerotii Poriae, inducing diuresis to remove edema is used for the diseases such as the wet edema of water, is used for having a guilty conscience palpitation with fear, and is forgetful, insomnia, infantile convulsion, dysuria." not Lu ": treat dizziness due to wind pathogen, wind be empty, five kinds of over strain, xerostomia.Spasmolytic is throbbed with fear, and is enraged more, and is forgetful.Spirit is supported in happy Fructus Alpiniae Oxyphyllae.

Rhizoma Atractylodis Macrocephalae bitter in the mouth, sweet, temperature.Return spleen, stomach warp.Cure mainly invigorating the spleen and benefiting QI, the dampness diuretic, hidroschesis, antiabortive.Be used for insufficiency of the spleen lack of appetite, abdominal distention is had loose bowels, phlegm retention vertigo and palpitation, edema, spontaneous perspiration, frequent fetal movement.The Rhizoma Atractylodis Macrocephalae has cytotoxicity to oncocyte, can reduce the rate of increase of oncocyte, lowers the aggressive of tumor tissue, improves the ability of body antitumor reaction.Rhizoma Atractylodis Macrocephalae volatile oil has inhibitory action to mice ehrlich carcinoma, lymphosarcoma ascitic type, the esophageal carcinoma (Ecal09), sarcoma 180 etc.Atractylodes lactone B lumbar injection is to the murine sarcoma people.Remarkable inhibitory action is also arranged." property of medicine opinion " carries it: monarch, and sweet in the mouth, suffering, nontoxic.Can main large migratory arthralgia, diarrhea due to disorder of qi for many years, trusted subordinate's distending pain, the broken dyspepsia that disappears, sputum is removed in appetizing, except cold and heat, lets out under ending.Interarea light is pleased, and preserves youthful looks, and goes to deceive.The swelling of harnessing the river is full, tells vomiting, and cold type of pain in the abdomen is vomited and diarrhoea incessantly, and the insufficiency of stomach-QI cold-dysentery.

Radix Salviae Miltiorrhizae is bitter, is slightly cold.GUIXIN, Liver Channel.Stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, relieving restlessness clears away heart-fire.Be used for menoxenia, the amenorrhea dysmenorrhea, lump in the abdomen, breast ventral spine pain, pyretic arthralgia pain, skin infection swells and ache, dysphoria and insomnia.Be used for the costa sternales hypochondriac pain, rheumatic arthralgia, the lump in the abdomen caking, skin infection swells and ache, tumbling down is grieved, menoxenia, the amenorrhea dysmenorrhea, puerperal stasis of blood pain etc.Treatment costa sternales pain, lump in the abdomen caking, and menoxenia, amenorrhea dysmenorrhoea have good effect." detailed outline " carries: Radix Salviae Miltiorrhizae, by " the sensible opinion of married woman " cloud, SIWU TANG is controlled the gynecopathy, pays no attention to antenatal puerperal, through water what, all can be general, only flavor Radix Salviae Miltiorrhizae is loose, cures mainly identical with it.The lid Radix Salviae Miltiorrhizae can break stagnated blood, mends fresh blood, peaceful tire, and the stillborn fetus that falls ends the metrorrhagia dysentery, the regulating menstruation arteries and veins.The Main Compositions of Radix Salviae Miltiorrhizae TANSHINONES has blood vessel dilating, improves effects such as circulating, remove intravascular coagulation, has the effect of invigorating blood circulation trace, and can promote the liver blood circulation to be beneficial to liver cell regeneration.

The Radix Astragali has the effects such as invigorating QI to consolidate the body surface resistance, diuretic detumescent, expelling pus and toxin by strengthening QI, granulation promoting.The medicinal so far history in existing more than 2000 year of the Radix Astragali, modern study, the Radix Astragali contain the various trace elements such as Saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.The enhancing human body immunity function arranged, protect the liver, diuresis, defying age, anti-stress, blood pressure lowering and antibacterial action more widely.But the table excess pathogen is contained, retention of dampness due to stagnation of QI, food stagnation is stagnated, carbuncle from the beginning of or burst after the excess syndrome such as pyretic toxicity Shang Sheng.The Radix Astragali has invigorating QI to consolidate the body surface resistance, diuresis poison holding, evacuation of pus, the effect of expelling pus and promoting granulation.It is weak to be used for the deficiency of vital energy, anorexia and loose stool, and sinking of QI of middle-JIAO, the chronic diarrhea proctoptosis, the metrorrhagia of having blood in stool, the exterior deficiency spontaneous perspiration, carbuncle is difficult bursts, and burst for a long time and do not hold back, blood deficiency and yellow complexion, interior-heat is quenched one's thirst." herbal classic " record: " main carbuncle loses skin ulcer for a long time, the evacuation of pus pain relieving.Tonify deficiency, children's's all kinds of diseases and ailments." " Japan hanako materia medica " record: " help the gas strengthening bone and muscle, put on flesh and enrich blood.”

Radix Angelicae Sinensis, its sweet in the mouth and weighing, so specially can enrich blood, its gas is light and hot, thus again can promoting the circulation of blood, invigorating middle warmer has, in the row benefit is arranged, really the gas medicine in the blood, the also panacea in the blood.About mending to mend then of assistant, thus can support nutrition blood, the QI invigorating spermatogenesis, settling five organs, body by force, beneficial mind, all tangible deficient diseases, nothing is unsuitable.Assistant logical to attack then, so the pain relieving constipation of dispelling, relieving rigidity of muscles and joints is controlled contracture, paralysis, the card such as dry, puckery.Nutrient QI being deficient and exterior syndrome being not relieved person, assistant is with agent such as bavin, Pueraria lobota, fiber crops, osmanthus, the Biao Weire that can fall apart greatly, and show not hold back the person, and help with Radix Et Rhizoma Rhei and so on, again can consolidating superficial resistance.Precisely because gas Xin Erdong, so want its quiet person when keeping away it, the sliding benefaction of property is defecated and is not consolidated the person when keeping away it.All cloudy moderate heat Sheng persons, the active blood of Radix Angelicae Sinensis, also non-institute is suitable, cloudy deficiency of YANG of middle-JIAO person, Radix Angelicae Sinensis can nourish blood, and is indispensable.

The Radix Paeoniae Alba enters liver, spleen channel, nourishing blood to suppress the hyperactive liver, and slow middle pain relieving, yin fluid astringing is received antiperspirant.Control the breast abdomen pain over the hypochondriac region, dysentery stomachache, spontaneous sweating, fever due to yin deficiency, menoxenia, metrorrhagia, leukorrhagia, married woman's blood closes obstructed, and repercussive blood can lose pus, beneficial woman's blood." Japan hanako materia medica ": control wind, mend labor, all diseases of main woman, and antenatal rear all diseases, Tong Yueshui brings down a fever, relieving restlessness, QI invigorating, day hot disease of row, the pestilence miasma, mania and irritation, married woman's blood fortune, and hemorrhoidal hamorrhage are rushed down blood, hemorrhoid complicated by anal fistula.Carbuncle on the back, the skin ulcer scabies, headache makes eye bright, and conjunctival congestion is exerted meat.The many QI invigorating of redness of the skin or complexion person, white person controls blood.

Radix Rehmanniae Preparata has another name called Radix Rehmanniae Preparata or throws oneself on the ground, belong to goatweed, be a kind of first-class Chinese crude drug, have the YIN nourishing of enriching blood effect, can be used for blood deficiency and yellow complexion, dizzy, palpitation and insomnia, menoxenia, the diseases such as metrorrhagia, also can be used for hectic fever hectic fever due to YIN-deficiency, the night sweat of deficiency of kidney yin, the disease such as pass out semen, quench one's thirst, is one of deficiency syndrome class nonprescription drugs medicine LIUWEI DIHUANG WAN main component.This quality is moistened into kidney, kind nourishing kidney yin, and life essence-filling, marrow-benefitting is the key medicine of tonifying kidney-yin.Ancients call it " large tonifying five ZANG-organs is Kidney-Yin ", " mending greatly true water ".Normal be equal to usefulness with Rhizoma Dioscoreae, Fructus Corni, treat the hepatic and renal YIN deficiency, soreness of the waist and knees, seminal emission, night sweat, tinnitus, deafness and quench one's thirst etc., but invigorating the liver and kidney, beneficial marrow is such as LIUWEI DIHUANG WAN.

Flos Albiziae contains the Herba Albiziae glycoside, tannin, and resolving stagnation for tranquilization, the appetizing of regulating the flow of vital energy, activating collaterals to relieve pain is used for irritability, melancholy insomnia.It is uncomfortable in chest to control pent-up, and insomnia is forgetful, and acute conjunctivitis can settling five organs, and will, improving healthy complexion, have preferably keep fit, calm, the effect of calming the nerves, improve looks, also be to treat neurasthenic good merchantable brand.Also has clearing away summer-heat, the face-beautifying and spot-removing effect such as relieve the effect of alcohol." book on Chinese herbal medicine warp " said: " Flos Albiziae energy settling five organs, and will, improving healthy complexion." especially to the anemia of pregnant woman, have preferably keep fit, calm, the effect of calming the nerves, improve looks, also be to treat neurasthenic good merchantable brand.

Herba Epimedii is hot, sweet, warm, returns liver, kidney channel.The energy reinforcing the kidney and supporting YANG, strengthening the tendons and bones, expelling wind and removing dampness is apt to control insufficiency of kidney-YANG, impotence and seminal emission, enuresis frequent micturition; Rheumatic arthralgia, atrophic debility of bones paralysis disease." book on Chinese herbal medicine is stated " cloud " excessive sheep fire, " herbal classic " medicine master impotence is hindered absolutely, and " solar corona " be the first absolutely sun of its treatment man of saying also, and the woman is cloudy absolutely, and then meaning enters the gate of vitality, benefit kidney-Yang person is also.The lid gate of vitality be kidney-Yang in the kidney, namely the vigour of the person also, its so-called exhausted YANG exhaustion the moon, the vigour of basis not, how hiss in both withered.So-called physical strength profiting, strong will, and control the cold air consumptive disease, and the anxious card that waits of muscles and bones contraction, all it helps the event of vigour.Old man's dusk octogenarian, the middle age is forgetful, all Yuanyang decay and can not ascensionist also.Must know that this distinguishes the flavor of to reduce to liter, its liter.

Caulis Polygoni Multiflori nourishes heart, and calms the nerves, and collateral dredging dispels the wind, and the traditional Chinese medical science thinks that Caulis Polygoni Multiflori is flat nontoxic, and sweet and slightly bitter taste enters the heart, Liver Channel, and the effect of tranquilizing and blood nourishing, dispelling wind and removing obstruction in the collateral is arranged, and cures mainly the diseases such as deficiency of YIN insufficiency of blood, restlessness of asrhenia type and insomnia, rheumatic arthralgia, itchy skin eruption.Compatibility of drugs: the complex acid Semen Ziziphi Spinosae, grow the heart-yin, mind calming is refreshing; Join the Radix Rehmanniae, the nourishing YIN of nourishing blood; Join Radix Asparagi, Radix Ophiopogonis, clear asthenic fire, the moon nourishes heart; Join Rhizoma Et Radix Notopterygii, Radix Angelicae Pubescentis, expelling wind and dampness, Shu Li joint.

Specific embodiment 1:

Preparation: get fresh Folium Ginkgo, clean, dry, in 70 ℃ of baking ovens, baking 9h (it is fixed that the time is looked the sample moisture, can 8～12h), and take out and place cooling in the drying baker, pulverized 60 mesh sieves with pulverizer, powder places brown wide mouthed bottle to store.

Lixiviate: prepare 60% alcoholic solution (get 640mL95% ethanol, the 360mL pure water is made into 60% alcoholic solution).Get the 100g ginkgo leaf powder in 700mL60% ethanol, in water-bath, be heated to 60 ℃～70 ℃, lixiviate 2h, every 10min stirs once, use filtered through gauze, add 60% ethanol 700mL in the residue, 60 ℃～70 ℃ are continued lixiviate 2h, every 10min stirs once, and filtered through gauze merges lixiviating solution.Do simultaneously parallel laboratory test.

Sucking filtration: use first the gauze coarse filtration, remove the bulky grain precipitation, to improve sucking filtration speed.The sucking filtration instrument is installed, and adding a small amount of lixiviating solution in the buchner funnel carries out sucking filtration, owing to contain gelatinous precipitate in the Folium Ginkgo, filter paper easily results in blockage, and causes the decline of sucking filtration speed, so should often change filter paper.Sucking filtration is got filtrate and is abandoned filtering residue after finishing.

Distillation, concentrated: distillation, concentrating appts are installed, are contained a large amount of alcoholic solution in the sucking filtration liquid, carry out ethanol with distilling apparatus and concentrate.A certain amount of ethanol also has bactericidal action, when measuring the extract bacteriostasis, should get rid of the interference of alcohol solvent as far as possible, and ethanol is volatilized totally fully.In order not destroy effective ingredient in the extract, bath temperature is controlled at 60 ℃～70 ℃, and the boiling point of 95% ethanol is about 75 ℃, so distillation speed is slow, the time is long.Can take simple method, direct heating in water bath, the ethanol that in air, volatilizees, this method shortcoming: can not Recycled ethanol.The extract that finally obtains is part precipitation extractum, and color is the dark-brown dope.

Dry: owing to still contain part ethanol and moisture in the concentrated lixiviating solution, adopt boulton process, temperature is controlled between 60 ℃～70 ℃, and utilization increases vacuum and reduces boiling point, removes last ethanol and residual part moisture.If conditions permit can also adopt freezing and drying lyophilization dry.Except containing flavonoid and lactone, also contain a certain amount of impurity in the extract of this moment.

All the other raw materials are granulated: with all the other raw material Radix Polygalaes, and Radix Rehmanniae Preparata, Radix Angelicae Sinensis, Fructus Corni, Radix Salviae Miltiorrhizae, Poria cum Radix Pini, Semen Juglandis, Radix Codonopsis, Semen Platycladi, Herba Epimedii, the Radix Astragali, Radix Polygoni Multiflori Preparata, the Radix Paeoniae Alba, Fructus Ligustri Lucidi, Semen Pruni, Flos Albiziae, Caulis Polygoni Multiflori is made extractum.

Packing: with the above-mentioned extractum that makes, mix with aforementioned Folium Ginkgo extract extractum, can add the agent of an amount of dextrin granulation, add starch and make tablet, add Mel and make pill or directly make powder, oral solutions.

Specific embodiment 2:

Pluck the fresh and tender Folium Ginkgo of 1000g, clean, dry, in 70 ℃ of baking ovens, baking 9h takes out and places cooling in the drying baker, pulverizes 60 mesh sieves with pulverizer, powder places brown wide mouthed bottle storage preparation 60% alcoholic solution (get 640mL95% ethanol, the 360mL pure water is made into 60% alcoholic solution).Get the 1000g ginkgo leaf powder in 7000mL60% ethanol, in water-bath, be heated to 60 ℃～70 ℃, lixiviate 2h, every 10min stirs once, use filtered through gauze, add 60% ethanol 7000mL in the residue, 60 ℃～70 ℃ are continued lixiviate 2h, every 10min stirs once, and filtered through gauze merges lixiviating solution.Do simultaneously parallel laboratory test.Use first the gauze coarse filtration, remove the bulky grain precipitation, to improve sucking filtration speed.The sucking filtration instrument is installed, and adding a small amount of lixiviating solution in the buchner funnel carries out sucking filtration, owing to contain gelatinous precipitate in the Folium Ginkgo, filter paper easily results in blockage, and causes the decline of sucking filtration speed, so should often change filter paper.Sucking filtration is got filtrate and is abandoned filtering residue after finishing.Distillation, concentrating appts are installed, are contained a large amount of alcoholic solution in the sucking filtration liquid, carry out ethanol with distilling apparatus and concentrate.A certain amount of ethanol also has bactericidal action, when measuring the extract bacteriostasis, should get rid of the interference of alcohol solvent as far as possible, and ethanol is volatilized totally fully.In order not destroy effective ingredient in the extract, bath temperature is controlled at 60 ℃～70 ℃, and the boiling point of 95% ethanol is about 75 ℃, so distillation speed is slow, the time is long.Can take simple method, direct heating in water bath, the ethanol that in air, volatilizees, this method shortcoming: can not Recycled ethanol.The extract that finally obtains is part precipitation extractum, and color is the dark-brown dope.Owing to still contain part ethanol and moisture in the concentrated lixiviating solution, adopt boulton process, temperature is controlled between 60 ℃～70 ℃, utilizes increase vacuum to reduce boiling point, removes last ethanol and residual part moisture.If conditions permit can also adopt freezing and drying lyophilization dry.With all the other raw material Radix Polygalae 500g, Radix Rehmanniae Preparata 500g, Radix Angelicae Sinensis 500g, Fructus Corni 500g, Radix Salviae Miltiorrhizae 500g, Poria cum Radix Pini 500g, Semen Juglandis 500g, Radix Codonopsis 500g, Semen Platycladi 500g, Herba Epimedii 500g, Radix Astragali 500g, Radix Polygoni Multiflori Preparata 500g, Radix Paeoniae Alba 500g, Fructus Ligustri Lucidi 500g, Semen Pruni 500g, Flos Albiziae 500g, Caulis Polygoni Multiflori 500g put it in 10 times of amount ethanol, heating and refluxing extraction 2 times each 1～2 hour, leaves standstill 2 extracting solution merging; The medicinal residues of the component raw medicinal material of more above-mentioned ethanol extraction being crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution merging are left standstill; Above-mentioned two kinds of extracting solution are merged; the concentrating under reduced pressure relative density is the filtrate of 1.36 (80 ℃); Recycled ethanol is adjusted proportion to 1.06 with gained filtrate, and extractum reduces pressure to get; putting into dehydrator dries; add dextrin, put into granulator and make 1-2 centimetre of granule, then mix with the Folium Ginkgo product extract finished product that above-mentioned steps obtains; be distributed into the essay bag of 10-15 gram, also can integral body be mixed into the bag sale.Can be distributed into the essay bag of 10-15 gram, also can integral body be mixed into the bag sale.

Specific embodiment 3:

Pluck the 500g Folium Ginkgo, clean, dry, in 70 ℃ of baking ovens, baking 9h takes out and places cooling in the drying baker, pulverized 60 mesh sieves with pulverizer, powder places brown wide mouthed bottle storage preparation 60% alcoholic solution (get 640mL95% ethanol, the 360mL pure water is made into 60% alcoholic solution).Get the 500g ginkgo leaf powder in 3500mL60% ethanol, in water-bath, be heated to 60 ℃～70 ℃, lixiviate 2h, every 10min stirs once, use filtered through gauze, add 60% ethanol 35000mL in the residue, 60 ℃～70 ℃ are continued lixiviate 2h, every 10min stirs once, and filtered through gauze merges lixiviating solution.Do simultaneously parallel laboratory test.Use first the gauze coarse filtration, remove the bulky grain precipitation, to improve sucking filtration speed.The sucking filtration instrument is installed, and adding a small amount of lixiviating solution in the buchner funnel carries out sucking filtration, owing to contain gelatinous precipitate in the Folium Ginkgo, filter paper easily results in blockage, and causes the decline of sucking filtration speed, so should often change filter paper.Sucking filtration is got filtrate and is abandoned filtering residue after finishing.Distillation, concentrating appts are installed, are contained a large amount of alcoholic solution in the sucking filtration liquid, carry out ethanol with distilling apparatus and concentrate.In order not destroy effective ingredient in the extract, bath temperature is controlled at 60 ℃～70 ℃, and the boiling point of 95% ethanol is about 75 ℃, so distillation speed is slow, the time is long.The extract that finally obtains is part precipitation extractum, and color is the dark-brown dope.Owing to still contain part ethanol and moisture in the concentrated lixiviating solution, adopt boulton process, temperature is controlled between 60 ℃～70 ℃, utilizes increase vacuum to reduce boiling point, removes last ethanol and residual part moisture.With all the other raw material Radix Polygalae 210g, Radix Rehmanniae Preparata 210g, Radix Angelicae Sinensis 220g, Fructus Corni 200g, Radix Salviae Miltiorrhizae 210g, Poria cum Radix Pini 240g, Semen Juglandis 250g, Radix Codonopsis 210g, Semen Platycladi 250g, Herba Epimedii 240g, Radix Astragali 250g, Radix Polygoni Multiflori Preparata 250g, Radix Paeoniae Alba 230g, Fructus Ligustri Lucidi 230g, Semen Pruni 250g, Flos Albiziae 240g, Caulis Polygoni Multiflori 230g put it in 10 times of water gagings and soak.Drop into multi-function extractor and extract twice, add 8-10 times of water gaging for the first time and decocted 1-2 hour, add 4-8 times of water gaging for the second time and decocted 1-2 hour, merge decocting liquid twice, filter to get filtrate; Heating is concentrated into pasty state, cools off after putting into 80 ℃ of oven dry of baking oven, is ground into fine powder.Then mix with the Folium Ginkgo extract that above-mentioned steps obtains, be distributed into the essay bag of 10-15 gram, also can integral body be mixed into the bag sale.Can be distributed into the essay bag of 10-15 gram, also can integral body be mixed into the bag sale.

Pharmacology's toxicity test

Health product acute toxicity test of the present invention

One, test material:

Animal: Kunming mouse, body weight 21-24g, male and female half and half, Shandong University's biology laboratory breeding.Medicine: health product of the present invention contain 0.0365g crude drug/g.

Two, method:

1, LD50 calculates: adopt the improvement karber's method, mice is divided into 5 groups at random, 10 every group, male and female half and half with adding distil water dissolving of the present invention, are made into Cmax, by the administration of the maximum permission capacity of mice, institute is followed successively by 18,14.4 to dosage by the crude drug amount, 11.5,9.2,7.4 (g.kg-1), in animal fasting (can't help water) after 18 hours, at twice administration in one day (interval half an hour), each 0.5ml observes the animal dead situation.

2, maximum tolerated dose is measured (MTD value): get 20 of mices, each 10 of male and female.With adding distil water of the present invention dissolving, be made into maximum concentration, by the maximum tolerated dose of animal, fill with injection and to feed device and can twitch and be as the criterion.In animal fasting (can't help water) after 18 hours, at twice feedwater (interval half an hour) in a day, each 0.5ml (every ml contains crude drug 0.038g), total dose is 19g crude drug/kg.d, 300 times of quite clinical adult 50Kg body weight consumption.Continuous Observation is 7 days after the administration.

Three, result of the test:

In LD50 calculates when giving mice with maximum acceptable concentration and maximum permission capacity (19g/Kg.d), have no dead mouse, namely do not measure LD50, ask only maximum tolerated dose, in 7 day observation period, its appetite of animal, activity, hair color, the mental status etc. are all normal, grow normally, there are no death.Namely select 300 times of doses that are equivalent to clinical dosage, and have no adverse reaction, show that acute toxicity is minimum, MTD＞18g/Kg.d.

Long term toxicity test data of the present invention

Chinese medicine of the present invention is through 6 months long-term toxicity test for animals result of gastric infusion: the SD rat is divided into three groups, 30 every group, male and female half and half (being divided into high, medium and low three dosage groups, 10 every group), wherein first group through gavage to normal saline; Second group is given dosage of the present invention through gavage is 0.25g, 2.5g and 12.5g/kg.d; The 3rd group is 0.5mg, 5.0mg and 50.0mg/kg.d through gavage to the dosage of lovastatin.During administration, observe respectively that clinical manifestation, hematology, blood after each treated animal administration is biochemical, the indexs such as organ coefficient and histopathology carry out the evaluation of drug toxicity.Result of study: main toxic reaction shows lovastatin 50mg/kg.d and 5.0mg group, shows after the administration that reaction and the symptoms such as sialorrhea, hair are fluffy, busy, diarrhoea, step have appearred in the SD rat, total food ration with lose weight; Blood test shows that total white blood cells increases to some extent, erythrocyte sum, content of hemoglobin and platelet count obviously descend (P all＜0.05); Blood biochemistry checking shows that ALT, AST, ALP and CK raise 1～8 times (P all＜0.05), and creatinine and blood urea nitrogen raise (P all＜0.05) to some extent, but do not cause animal dead; Histopathological analysis result shows that the Liver and kidney pathological change similar to the people appearred in lovastatin 50mg/kg.d group.And similar reaction does not all appear in three dosage groups of normal saline group and 0.25g of the present invention, 2.5g and 12.5g/kg.d and lovastatin 0.5mg/kg.d dosage group during whole administration, at the convalescent period viewing duration, the symptom that each treated animal of reaction the occurs recovery normal condition that fades away.As seen the present invention not only can reduce the toxic and side effects of lovastatin, and can make the clinical non-toxic of lovastatin increase to 5～25 times (P all＜0.01).

The specific embodiment:

At present, the health product of domestic prevention senile dementia are very fiery, and the articles for use of Chinese herb on the prevention senile dementia also are a dark horse, sweep the country. but health product is to the research of hypophysis cerebri effect, be main mainly with clinical observation on the therapeutic effect greatly, the animal experiment study that less employing is tight, so that science is not strong, cogency is not enough.The present invention comes verification the verifying results with zoopery.

The test material of raising memory function of the present invention and method

1.1 material: the capsule of embodiment of the invention preparation; Positive drug is: Tianwang Buxin Dan, and commercially available.

1.1.1 Chinese medicine composition of the present invention causes the impact of mouse memory acquired disturbance on scopolamine

1.1.2. animal grouping, administration

Get 60 of female KM mices, body weight (20 ± 2) g, be divided at random Normal group, model group, positive drug group and be subjected to the large, medium and small dosage group of reagent by body weight, 10/, each treated animal gastric infusion, the large, medium and small dosage of preparation of the present invention are equivalent to respectively 10 times, 5 times, 2.5 times of people's clinical application amount, matched group and model group gavage equivalent ordinary water, 1 times/day, continuous 10 days.Each is organized mice diet drinking-water and is not limit.

1.1.3. index determining

Each treated animal respectively at the last administration after 1h, all the other respectively organize mouse stomach scopolamine 3mg/kg except normal group, carry out diving tower training behind the 10min, adapt to 3min, training 5min re-starts test behind the 24h, mice is placed on the diving tower wrong in the record 5min (coming from diving tower) number of times.

1.1.4 result of the test the results are shown in Table 1.

Table 1 Chinese medicine composition of the present invention causes the impact of mouse memory acquired disturbance on scopolamine

Annotate: compare * P＜0.05, * * P＜0.01, * * * P＜0.001 with Normal group; Compare △ P＜0.05, △ △ P＜0.01, △ △ △ P＜0.001 with model group.

The result shows that the model group mouse wrong times increases, but compares with Normal group, and difference there is no significant, and each administration group mice and model group comparison error number of times reduce, and difference there is no significant.

The result shows that preparation of the present invention causes memory deficits in mice to scopolamine and has some improvement.

2.1 preparation of the present invention is on the impact of castration mouse learning and memory function

2.1.2. animal grouping, administration

Get 50 of female KM mices, body weight (20 ± 2) g, the castration modeling is the same, checks that the castration eligible is used for test, be divided at random model group, positive drug group and be subjected to the large, medium and small dosage group of reagent by body weight, 10/, other gets 10 female mices (not doing the castration operation) and does Normal group, in postoperative beginning in the 9th day administration, dosage is with 1.1,1 times/day, continuous 10 days, each was organized mice diet drinking-water and is not limit.

2.1.3. index determining

Each treated animal is trained before respectively at the last administration, tests Memory result behind the administration 24h, surveys to jump off platform for the 1st time and starve errors number in incubation period and the 5min.

2.2. result of the test the results are shown in Table 2.

Table 2 impact on the castration mouse memory function of the present invention

Annotate: compare * P＜0.05, * * P＜0.01, * * * P＜0.001 with Normal group; Compare △ P＜0.05, △ △ P＜0.01, △ △ △ P＜0.001 with model group.

The result shows that castration model mice errors number increases, and compared with normal matched group difference has significant (P＜0.05); The heavy dose of group of preparation of the present invention mouse wrong times reduces than model group, and difference has significant (P＜0.05).

The result shows that the present invention can significantly improve castration model mice memory function.The medicine focus of going directly, QI and blood regulating, Yin Yang balancing strengthen the brain cell contractile function on the one hand, improve the situation of qi depression to blood stasis, venation block, make QI and blood keep unimpeded, ensure the blood oxygen of brain supply; Repair on the other hand and activate impaired brain cell tissue, recover the brain normal function.

A kind of composition is arranged ginkgoic acid in the Folium Ginkgo, if the dosage of usefulness is excessive, perhaps Time of Administration is long, can damage the heart old people and note regularly detecting the blood coagulation situation when taking Folium Ginkgo, not the blindness long-term taking.The patient who becomes fragile for existing senile sclerosis of blood vessels, blood vessel more can not long-term taking, in order to avoid cause cerebral hemorrhage.

Claims (10)

1. ginkgo Leaves health care product that prevent senile dementia, it is characterized in that the parts by weight of various raw materials are in the described prevention senile dementia ginkgo Leaves health care product: 10～30 parts of Folium Ginkgos, 10～20 parts of Radix Polygalaes, 10～20 parts in Radix Rehmanniae Preparata, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Fructus Corni, 10～20 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Poria cum Radix Pinis, 10～20 parts of Semen Juglandis, 10～20 parts of Radix Codonopsis, 10～20 parts of Semen Platycladi, 10～20 parts of Herba Epimedii, 10～20 parts of the Radixs Astragali, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Semen Prunis, 10～20 parts of Flos Albiziaes, 10～20 parts of Caulis Polygoni Multiflori.

2. the ginkgo Leaves health care product of described prevention senile dementia according to claim 1, it is characterized in that the parts by weight of various raw materials are in the described prevention senile dementia ginkgo Leaves health care product: 10～30 parts of Folium Ginkgos, 10～15 parts of Radix Polygalaes, 10～15 parts in Radix Rehmanniae Preparata, 10～15 parts of Radix Angelicae Sinensis, 10～15 parts of Fructus Corni, 10～15 parts of Radix Salviae Miltiorrhizaes, 10～15 parts of Poria cum Radix Pinis, 10～15 parts of Semen Juglandis, 10～15 parts of Radix Codonopsis, 10～20 parts of Semen Platycladi, 10～20 parts of Herba Epimedii, 10～20 parts of the Radixs Astragali, 10～20 parts of Radix Polygoni Multiflori Preparata, 10～20 parts of the Radix Paeoniae Albas, 10～20 parts of Fructus Ligustri Lucidi, 10～20 parts of Semen Prunis, 10～20 parts of Flos Albiziaes, 10～20 parts of Caulis Polygoni Multiflori.

3. prevent according to claim 1 the ginkgo Leaves health care product of senile dementia, it is characterized in that the parts by weight of various raw materials are in the described prevention senile dementia ginkgo Leaves health care product: 10～30 parts of Folium Ginkgos, 10～20 parts of Radix Polygalaes, 10～20 parts in Radix Rehmanniae Preparata, 10～20 parts of Radix Angelicae Sinensis, 10～20 parts of Fructus Corni, 10～20 parts of Radix Salviae Miltiorrhizaes, 10～20 parts of Poria cum Radix Pinis, 10～20 parts of Semen Juglandis, 10～20 parts of Radix Codonopsis, 10～15 parts of Semen Platycladi, 10～15 parts of Herba Epimedii, 10～15 parts of the Radixs Astragali, 10～15 parts of Radix Polygoni Multiflori Preparata, 10～15 parts of the Radix Paeoniae Albas, 10～15 parts of Fructus Ligustri Lucidi, 10～15 parts of Semen Prunis, 10～15 parts of Flos Albiziaes, 10～15 parts of Caulis Polygoni Multiflori.

4. prevent according to claim 1 the ginkgo Leaves health care product of senile dementia, it is characterized in that, the dosage form of described prevention senile dementia ginkgo Leaves health care product is: oral solutions, unguentum, tablet, pill or powder.

5. preparation method such as claim 1～4 the ginkgo Leaves health care product of prevention senile dementia as described in each is characterized in that the preparation method of described ginkgo Leaves health care product may further comprise the steps:

A. the Folium Ginkgo preparation is extracted;

B. the sucking filtration of Folium Ginkgo extract;

C. Folium Ginkgo extract is condensed into particulate matter, powder or unguentum;

D. with the agent of surplus stock component granulation, powder or unguentum.

E. pack after granule obtained above, powder or unguentum being mixed with granule, powder or the unguentum of the Folium Ginkgo extract of making.

6. the preparation method of the ginkgo Leaves health care product of described prevention senile dementia according to claim 5, it is characterized in that: among the described step a, get fresh Folium Ginkgo, clean, dry, in 70 ℃ of baking ovens, baking 8-12h takes out and places cooling in the drying baker, pulverizes the 80-100 mesh sieve with pulverizer, powder places 95% ethanol to soak 1-2 hour, in water-bath, be heated to 60 ℃～70 ℃, lixiviate 2h, every 10min stirs once, use filtered through gauze, add 60% ethanol in the residue, 60 ℃～70 ℃ are continued lixiviate 2h, and every 10min stirs once, filtered through gauze merges lixiviating solution.

7. the preparation method of the ginkgo Leaves health care product of described prevention senile dementia according to claim 5 is characterized in that: among the described step b, ginkgo biloba succi is used first the gauze coarse filtration, remove the bulky grain precipitation, to improve sucking filtration speed; The sucking filtration instrument is installed, and adding a small amount of lixiviating solution in the buchner funnel carries out sucking filtration, after the sucking filtration end, gets filtrate, abandons filtering residue.

8. the preparation method of the ginkgo Leaves health care product of described prevention senile dementia according to claim 5, it is characterized in that: among the described step c, the Folium Ginkgo slag that ethanol extraction is crossed is put into 10 times of water gagings, heating and refluxing extraction 1 time, extraction time is 1～2 hour, the 100-120 order filters, and is the ultrafiltration post ultrafiltration of 5000-10000 through the molecular weight that dams again, 1.38 filtrate when ultrafiltrate concentrating under reduced pressure relative density is 80 ℃; Merge twice filtrate heating and be concentrated into the extractum shape.

9. the preparation method of the ginkgo Leaves health care product of described prevention senile dementia according to claim 5, it is characterized in that: in the described steps d, the surplus stock component is put into 10 times of amount ethanol, heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution merging are left standstill; The medicinal residues of the component raw medicinal material of more above-mentioned ethanol extraction being crossed add 10 times of water gaging heating and refluxing extraction 2 times, each 1～2 hour, 2 extracting solution merging are left standstill; Above-mentioned two kinds of extracting solution are merged, 1.36 o'clock filtrate when the concentrating under reduced pressure relative density is 80 ℃, Recycled ethanol is adjusted proportion to 1.06 with gained filtrate, and extractum reduces pressure to get.

10. the preparation method of described Folium Ginkgo blood fat-reducing blood pressure-decreasing health product according to claim 5, it is characterized in that: among the described step e, merge making the Folium Ginkgo extract extractum that extractum and above-mentioned steps make in the steps d, fully mix granulation agent, powder, pill, tablet or unguentum.