CN103053310A - Method for cultivating vallisneria natans seedlings - Google Patents

Method for cultivating vallisneria natans seedlings Download PDF

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CN103053310A
CN103053310A CN2013100077719A CN201310007771A CN103053310A CN 103053310 A CN103053310 A CN 103053310A CN 2013100077719 A CN2013100077719 A CN 2013100077719A CN 201310007771 A CN201310007771 A CN 201310007771A CN 103053310 A CN103053310 A CN 103053310A
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seedling
seedlings
seedbed
centimetres
water
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CN103053310B (en
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李明亮
叶子军
宋新元
林虹
梁果风
伍波
张治军
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CHENGDU HUADU AGRICULTURAL DEVELOPMENT Co Ltd
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CHENGDU HUADU AGRICULTURAL DEVELOPMENT Co Ltd
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Abstract

The invention discloses a method for cultivating vallisneria natans seedlings. The method includes steps of digging a seedling bed with the depth ranging from 8 centimeters to 10 centimeters, smashing soil blocks, adding from 0.3 to 0.5 kilogram of humus soil to each square meter of seeding bed, uniformly stirring the humus soil in the seedling bed, adding water into a seedling culture field and adding bleaching powder into the water for disinfection; selecting vallisneria natans with plant heights ranging from 15 centimeters to 20 centimeters as annual seedlings, and placing and soaking the annual seedlings in a disinfection tank for 6 to 10 minutes; transplanting the annual seedlings on the seedling bed, dividing each three to five annual seedlings as a stump, spraying combination hormone liquid on the seedling bed and covering a thin film on the annular seedlings; controlling the temperature of the water of the seedling culture field to range from 15 DEG C to 30 DEG C; and removing the thin film covering the vallisneria natans seedlings, filling water into the seedling bed, keeping the seedlings in the seedling bed for 9 to 11 days and shifting the seedlings out of the seedling culture field to obtain the vallisneria natans seedlings. After the annual seedlings are transplanted in the seedling culture field for 48 to 52 days, plant heights of new vallisneria natans seedlings grown on underground stems can reach 10 centimeters. The method has the advantages that seasonal limitation on plantation of the vallisneria natans can be broken through, and shortcomings of high cost, long period, high operational requirements and the like of a tissue culture technology for the vallisneria natans can be overcome.

Description

The breeding method of eel grass seedling
Technical field
The embodiment of the invention relates to a kind of cultivation of plants method, is specifically related to a kind of breeding method of eel grass seedling.
Background technology
Eel grass (Vallisneria natans) is heavy this plant of pasture and water of Hydrocharctaceae Vallisneria, find to have now 5 kinds, comprise eel grass, close tooth eel grass, thorn eel grass, Asia eel grass and large eel grass, eel grass is permitted can both survive in the eurypalynous water body in China, and water environment is had wider tolerance range.Eel grass often is used as food resource and is used for propagating artificially fish, shrimps and crab, also being usually used in addition the Shui nationality views and admires, eel grass is as high submerged plant, one of primary producer in the aquatic ecosystem, the ability that has purification of water quality and slow down Nutrient release, algae is had allelopathy, can suppress the Growth and reproduction of algae.
Lake, Reservoir Eutrophication problem are serious in recent years, the Water Environment In Lakes problem of formation take nitrogen and phosphorus pollution as essential characteristic, it is serious to concentrate on the lake and reservoir eutrophication, the water and soil resources exploitation excessively, ecological water is tied up in a large number, the river is withered, the section in wetlands degradation, the city of flowing through is subject to severe contamination, and bio-diversity reduces, and aquatic ecosystem is subject to heavy damage.The Water Ecological Recovery technology is a kind of improvement body eutrophication problem forefront technology at present, and wherein utilizing the submerged plant eel grass to carry out Water Ecological Recovery is one of key content in this type of technology.
In addition, raising along with people's living standard, the demand of amusement landscape water body constantly increases, a large amount of man-made lakes and Small-scale Landscape Water Bodies are applied in the landscape construction, normal method by a large amount of planting aquatic plants, structure aquatic ecosystem, guarantee that water quality is steady in a long-term, wherein eel grass is one of main biomaterial.
At present the obtain manner of eel grass material has from river, lake, reservoir, culture pond and directly obtains, or indirectly obtain by the mode that hibernaculum, seed, tissue are cultivated, for the artificial collection of the natural eel grass that can directly gather through long-term extensive style, wild resource is exhausted in a large number at present; The limitation such as and the eel grass tissue culture technique exists the cost height, and the cycle is long, operation requirements is high.Along with progressively the increasing sharply of maturation, engineering successful case of aquatic ecological technology, the day by day increasing of Technique Popularizing demand, the eel grass demand will present the blowout formula and increase, and the Availability of eel grass resource can't satisfy engineering application in the future at present.
Summary of the invention
The object of the invention is to solve the defective of above-mentioned prior art, the breeding method of the eel grass seedling that a kind of cost is low, the cycle is short is provided.
For solving above-mentioned technical problem, the present invention by the following technical solutions:
A kind of breeding method of eel grass seedling may further comprise the steps:
Put the field of growing seedlings in order: the described field of growing seedlings is comprised of at least one seedbed, the seedbed is dug dark 8 to 10 centimetres, soil block is smashed, and every square metre of seedbed adds 0.3 to 0.5 kilogram of humus soil, stirs, the seedbed is put in order smooth, then add entry in the field of growing seedlings, the control water depth is 1 to 2 centimetre, bleaching powder is added to the water carry out disinfection, control every square metre of seedbed and add 5 to 10 gram bleaching powder, disinfecting time 3 to 5 days;
Select seedling: select the eel grass of 15 to 20 centimetres of plant heights as seedling, reject withered old blade and root, put into sterilization pool and soak 6 to 10 minutes kinds, thimerosal in the described sterilization pool is the liquor natrii hypochloritis of mass fraction 1%, described seedling is wild eel grass seedling preferably, wild eel grass seedling early growth is energetic, without insect pest;
Transplant seedling: seedling is transplanted on the seedbed, per 3 to 5 strain seedlings are a root and stem of certain plants, the line-spacing of every root and stem of certain plants is 28 to 32 centimetres, the row distance is 28 to 32 centimetres, the depth of water is 8 to 12 centimetres in the control seedbed, on the seedbed, spray hormone combinations liquid, the amount of described hormone combinations liquid is 0.1 liter of every square metre of seedbed spray, then cover film on seedling, keep water temperature 15 to 30 degree in the seedbed, described hormone combinations liquid is to add 15 to 20 milligrams 6-benzyl aminoadenine and 8 to 12 milligrams the formed solution of indole-3-acetic acid in every premium on currency; The eel grass seedling forms new plant by subterranean stem, and connect together with seedling, division propagation refers to that the seedling that will naturally connect together separately forms independently plant, can promote so new rhizomatous growth, selecting per 3 to 5 strain seedlings is root and stem of certain plants plantation, both can save workload, be beneficial to again rhizomatous growth;
Manage the field of growing seedlings: the water temperature of controlling the field of growing seedlings is 15 to 30 degree, the front 30 days seedbed depth of waters behind the transplanting seedling are controlled at 8 to 12 centimetres, transplant seedling after 30 days the seedbed depth of water be controlled at 19 to 21 centimetres, control pH value is 6 to 8, seedling is transplanted into grew seedlings the field in the time of 48 to 52 days, the new eel grass seedling that forms by subterranean stem will grow to 10 centimetres of plant heights, the eel grass seedling density in seedbed be 250 to 300 strains/square metre;
Shift out seedling: the film that will cover on the eel grass seedling is removed, and adds water in the seedbed, makes the depth of water reach 28 to 32 centimetres, kept 9 to 11 days, if during find that attachment and insect pest then remove attachment and insect pest, seedling is shifted out the field of growing seedlings, namely obtain the eel grass seedling.
As optimal way of the present invention, in the breeding method of above-mentioned eel grass seedling, preferably, the soil property in the described field of growing seedlings is neutrality or slant acidity, and more preferably, the physical features in the field of growing seedlings is smooth, leeward on the sunny side, draining is good.
As optimal way of the present invention, in the breeding method of above-mentioned eel grass seedling, preferably, the length in the described field of growing seedlings is 30 to 40 meters, and width is 6 to 7 meters, and the field retaining degree of depth of growing seedlings is 40 to 50 centimetres, and the steel skeleton booth is adopted in the field of growing seedlings.
Effect of the present invention and advantage are as follows: the present invention utilizes the division propagation of eel grass self and raise seedling in greenhouse field to cultivate the eel grass seedling, energy amount reproduction eel grass seedling, and cost is low, and the eel grass survival rate is high, pollutes and lacks, and insect pest is few, suits large area to popularize; It is required that method of the present invention has satisfied four seasons of aquatic ecosystem structure, reparation, regulation and control, broken the season limit of eel grass plantation, overcome its tissue culture technique and had the shortcomings such as cost is high, the cycle is long, operation requirements is high, and optimized the form of construction work of eel grass in the Aquatic Ecosystem 2 systems engineering Xi.
Embodiment
According to one embodiment of present invention, the selection physical features is smooth, leeward facing south, draining is good, have abundant water resources, convenient transportation, soil property is fertile to be the filling out as growing seedlings the field of neutrality or slant acidity, the length in the described field of growing seedlings is 30 meters, width is 6 meters, and the field retaining degree of depth of growing seedlings is 40 centimetres, and the steel skeleton booth is adopted in the field of growing seedlings, the seedbed is dug dark 8 centimetres, break up the clods, every square metre of seedbed adds 0.3 kilogram of humus soil, stirs, the seedbed is put in order smooth, then add entry in the seedbed, the control water depth is 1 centimetre, bleaching powder is added to the water carry out disinfection, control every square metre of seedbed and add 5 gram bleaching powder, disinfecting time 5 days; It is strong to gather 2000 strain growth vigors, and without insect pest, the eel grass that plant height is 15 centimetres is rejected withered old blade and root as seedling, puts into sterilization pool and soaks 6 minutes kinds, and the thimerosal in the described sterilization pool is the liquor natrii hypochloritis of mass fraction 1%; Seedling is transplanted on the seedbed, per 3 strain seedlings are a root and stem of certain plants, the line-spacing of every root and stem of certain plants is 28 centimetres, row are apart from being 28 centimetres, and the depth of water is 8 centimetres in the control seedbed, spray hormone combinations liquid on the seedbed, described hormone combinations liquid is to add 15 milligrams 6-benzyl aminoadenine and 8 milligrams the formed solution of indole-3-acetic acid in every premium on currency, the amount of described hormone combinations liquid is 0.1 liter of every square metre of seedbed spray, and then cover film on seedling keeps water temperature 15 degree in the seedbed; The water temperature of controlling the field of growing seedlings is 15 degree, the front 30 days depth of waters behind the transplanting seedling are controlled at 8 centimetres, transplant seedling after 30 days the depth of water be controlled at 19 centimetres, control pH value is 6, seedling is transplanted into grew seedlings the field in the time of 48 days, the new eel grass seedling that forms by subterranean stem will grow to 10 centimetres of plant heights, the eel grass seedling density in seedbed be 250 strains/square metre; The film that covers on the eel grass seedling is removed, in the field of growing seedlings, add water, make the depth of water reach 28 centimetres, hardening 9 days, seedling is shifted out the field of growing seedlings, namely obtain 10000 strain eel grass seedlings, the eel grass seedling of cultivating is applied in certain golf landscape lake aquatic ecosystem recovery project-regulation and control in the engineering, the survival rate of this commodity eel grass seedling is 99.3%.
According to one embodiment of present invention, the selection physical features is smooth, leeward facing south, draining is good, have abundant water resources, convenient transportation, soil property is fertile to be the filling out as growing seedlings the field of neutrality or slant acidity, the length in the described field of growing seedlings is 35 meters, width is 6.5 meters, and the field retaining degree of depth of growing seedlings is 45 centimetres, and the steel skeleton booth is adopted in the field of growing seedlings, the seedbed is dug dark 9 centimetres, break up the clods, every square metre of seedbed adds 0.4 kilogram of humus soil, stirs, the seedbed is put in order smooth, then add entry in the seedbed, the control water depth is 1.5 centimetres, bleaching powder is added to the water carry out disinfection, control every square metre of seedbed and add 8 gram bleaching powder, disinfecting time 4 days; It is strong to gather 1000 strain growth vigors, and without insect pest, the eel grass that plant height is 18 centimetres is rejected withered old blade and root as seedling, puts into sterilization pool and soaks 8 minutes kinds, and the thimerosal in the described sterilization pool is the liquor natrii hypochloritis of mass fraction 1%; Seedling is transplanted on the seedbed, per 4 strain seedlings are a root and stem of certain plants, the line-spacing of every root and stem of certain plants is 30 centimetres, row are apart from being 30 centimetres, and the depth of water is 10 centimetres in the control seedbed, spray hormone combinations liquid on the seedbed, described hormone combinations liquid is to add 18 milligrams 6-benzyl aminoadenine and 10 milligrams the formed solution of indole-3-acetic acid in every premium on currency, the amount of described hormone combinations liquid is 0.1 liter of every square metre of seedbed spray, and then cover film on seedling keeps water temperature 25 degree in the seedbed; The water temperature of controlling the field of growing seedlings is 25 degree, the front 30 days depth of waters behind the transplanting seedling are controlled at 10 centimetres, transplant seedling after 30 days the depth of water be controlled at 20 centimetres, control pH value is 7, seedling is transplanted into grew seedlings the field in the time of 50 days, the new eel grass seedling that forms by subterranean stem will grow to 10 centimetres of plant heights, the eel grass seedling density in seedbed be 280 strains/square metre; The film that covers on the eel grass seedling is removed, in the field of growing seedlings, add water, make the depth of water reach 30 centimetres, hardening 10 days shifts out the field of growing seedlings with seedling, namely obtains 5000 strain eel grass seedlings, 5000 strain eel grass seedlings are moved in the plantation field, continued growth is kept a full stand of seedings, and increases simultaneously to expand the field cyclic seedling cultivation and keeping a full stand of seedings of growing seedlings, through cultivating altogether 10000000 strain eel grass seedlings in 2 years.The commodity eel grass seedling of cultivating is applied in certain artificial lake water ecosystem makes up in engineering-regulation and control in the engineering, the survival rate of this commercial seedling is 99.5%.
According to one embodiment of present invention, the selection physical features is smooth, leeward facing south, draining is good, have abundant water resources, convenient transportation, soil property is fertile to be the filling out as growing seedlings the field of neutrality or slant acidity, the length in the described field of growing seedlings is 40 meters, width is 7 meters, and the field retaining degree of depth of growing seedlings is 50 centimetres, and the steel skeleton booth is adopted in the field of growing seedlings, the seedbed is dug dark 10 centimetres, break up the clods, every square metre of seedbed adds 0.5 kilogram of humus soil, stirs, the seedbed is put in order smooth, then add entry in the seedbed, the control water depth is 2 centimetres, bleaching powder is added to the water carry out disinfection, control every square metre of seedbed and add 10 gram bleaching powder, disinfecting time 3 days; It is strong to gather 2000 strain growth vigors, and without insect pest, the eel grass that plant height is 20 centimetres is rejected withered old blade and root as seedling, puts into sterilization pool and soaks 10 minutes kinds, and the thimerosal in the described sterilization pool is the liquor natrii hypochloritis of mass fraction 1%; Seedling is transplanted on the seedbed, per 5 strain seedlings are a root and stem of certain plants, the line-spacing of every root and stem of certain plants is 32 centimetres, row are apart from being 32 centimetres, and the depth of water is 12 centimetres in the control seedbed, spray hormone combinations liquid on the seedbed, described hormone combinations liquid is to add 20 milligrams 6-benzyl aminoadenine and 12 milligrams the formed solution of indole-3-acetic acid in every premium on currency, the amount of described hormone combinations liquid is 0.1 liter of every square metre of seedbed spray, and then cover film on seedling keeps water temperature 30 degree in the seedbed; The water temperature of controlling the field of growing seedlings is 30 degree, the front 30 days depth of waters behind the transplanting seedling are controlled at 12 centimetres, transplant seedling after 30 days the depth of water be controlled at 21 centimetres, control pH value is 8, seedling is transplanted into grew seedlings the field in the time of 52 days, the new eel grass seedling that forms by subterranean stem will grow to 10 centimetres of plant heights, the eel grass seedling density in seedbed be 300 strains/square metre; The film that covers on the eel grass seedling is removed, in the field of growing seedlings, add water, make the depth of water reach 32 centimetres, hardening 11 days, seedling is shifted out the field of growing seedlings, namely obtain 10000 strain eel grass seedlings, the eel grass seedling of cultivating is applied in certain golf landscape lake aquatic ecosystem recovery project-regulation and control in the engineering, the survival rate of this commodity eel grass seedling is 99.5%.
" embodiment " who speaks of in this manual, " another embodiment ", " embodiment ", etc., refer to specific features, structure or the characteristics described in conjunction with this embodiment and be included among at least one embodiment that the application's generality describes.A plurality of local appearance statement of the same race is not necessarily to refer to same embodiment in specification.Furthermore, when describing a specific features, structure or characteristics in conjunction with arbitrary embodiment, what advocate is to realize that in conjunction with other embodiment this feature, structure or characteristics also fall within the scope of the invention.
Although invention has been described with reference to a plurality of explanatory embodiment of the present invention here, but, should be appreciated that those skilled in the art can design a lot of other modification and embodiments, these are revised and embodiment will drop within the disclosed principle scope and spirit of the application.

Claims (3)

1. the breeding method of an eel grass seedling is characterized in that, may further comprise the steps:
Put the field of growing seedlings in order: the described field of growing seedlings is comprised of at least one seedbed, the seedbed is dug dark 8 to 10 centimetres, soil block is smashed, and every square metre of seedbed adds 0.3 to 0.5 kilogram of humus soil, stirs, the seedbed is put in order smooth, then add entry in the field of growing seedlings, the control water depth is 1 to 2 centimetre, bleaching powder is added to the water carry out disinfection, control every square metre of seedbed and add 5 to 10 gram bleaching powder, disinfecting time 3 to 5 days;
Select seedling: select the eel grass of 15 to 20 centimetres of plant heights as seedling, put into sterilization pool and soak 6 to 10 minutes kinds, the thimerosal in the described sterilization pool is the liquor natrii hypochloritis of mass fraction 1%;
Transplant seedling: seedling is transplanted on the seedbed, per 3 to 5 strain seedlings are a root and stem of certain plants, the line-spacing of every root and stem of certain plants is 28 to 32 centimetres, the row distance is 28 to 32 centimetres, the depth of water is 8 to 12 centimetres in the control seedbed, on the seedbed, spray hormone combinations liquid, the amount of described hormone combinations liquid is 0.1 liter of every square metre of seedbed spray, then cover film on seedling, keep water temperature 15 to 30 degree in the seedbed, described hormone combinations liquid is to add 15 to 20 milligrams 6-benzyl aminoadenine and 8 to 12 milligrams the formed solution of indole-3-acetic acid in every premium on currency;
Manage the field of growing seedlings: the water temperature of controlling the field of growing seedlings is 15 to 30 degree, the front 30 days seedbed depth of waters behind the transplanting seedling are controlled at 8 to 12 centimetres, transplant seedling after 30 days the seedbed depth of water be controlled at 19 to 21 centimetres, control pH value is 6 to 8, seedling is transplanted into grew seedlings the field in the time of 48 to 52 days, the new eel grass seedling that forms by subterranean stem will grow to 10 centimetres of plant heights, the eel grass seedling density in seedbed be 250 to 300 strains/square metre;
Shift out seedling: the film that will cover on the eel grass seedling is removed, and adds water in the seedbed, makes the depth of water reach 28 to 32 centimetres, keeps 9 to 11 days, and seedling is shifted out the field of growing seedlings, and namely obtains the eel grass seedling.
2. the breeding method of described eel grass seedling according to claim 1 is characterized in that the soil property in the described field of growing seedlings is neutrality or slant acidity.
3. the breeding method of described eel grass seedling according to claim 1 is characterized in that the length in the described field of growing seedlings is 30 to 40 meters, and width is 6 to 7 meters, and the field retaining degree of depth of growing seedlings is 40 to 50 centimetres.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107580985A (en) * 2017-09-28 2018-01-16 昭平县全盛农业科技发展有限责任公司 A kind of breeding method of eel grass seedling
CN115581175A (en) * 2022-11-03 2023-01-10 中国科学院水生生物研究所 Method for promoting germination of submerged plant tape grass seeds and growth of seedlings

Citations (1)

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Publication number Priority date Publication date Assignee Title
CN102177806A (en) * 2011-03-30 2011-09-14 中国科学院水生生物研究所 Rapid field planting method for eel grass

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN102177806A (en) * 2011-03-30 2011-09-14 中国科学院水生生物研究所 Rapid field planting method for eel grass

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刘敏: "苦草的生物学特性及其在水产养殖上的应用", 《内陆水产》 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107580985A (en) * 2017-09-28 2018-01-16 昭平县全盛农业科技发展有限责任公司 A kind of breeding method of eel grass seedling
CN115581175A (en) * 2022-11-03 2023-01-10 中国科学院水生生物研究所 Method for promoting germination of submerged plant tape grass seeds and growth of seedlings

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