CN103044096A - Solid-state compound actinomycetes bacterial fertilizer and preparation method thereof - Google Patents
Solid-state compound actinomycetes bacterial fertilizer and preparation method thereof Download PDFInfo
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- CN103044096A CN103044096A CN2012104078699A CN201210407869A CN103044096A CN 103044096 A CN103044096 A CN 103044096A CN 2012104078699 A CN2012104078699 A CN 2012104078699A CN 201210407869 A CN201210407869 A CN 201210407869A CN 103044096 A CN103044096 A CN 103044096A
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- streptomycete
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- actinomycetes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a solid-state compound actinomycetes bacterial fertilizer and a preparation method thereof. The solid-state compound actinomycetes bacterial fertilizer is obtained by performing solid fermentation on actinomycetes separated from fresh excrement of free-ranging wild animals in a wildlife park and mixing proportionally. If the bacterial fertilizer obtained by the method provided by the invention is applied to planting pseudo-ginseng, the seedling keeping rate and the transplanting survival rate of the pseudo-ginseng can be increased.
Description
Technical field
The invention belongs to farm crop fertilizer, specifically a kind of solid union actinomycetes bacterial manure and preparation method thereof.
Background technology
Actinomycetes are as the microorganism that extensively exists in the class soil, and the effect research that its ecological functions in soil reach plant growth not yet obtains deeply.Some agricultural special product crops, such as pseudo-ginseng, ginseng, Radix Panacis Quinquefolii etc. because the plantation of need to shading for a long time of species characteristic, irrigation intensity greatly and the soil humidity that arrives is high, acute variation is occuring in the structure of soil and rhizospheric microorganism group.Harmful germ amount reproduction, thus the popular of crop pest and outburst caused, cause Severe Reduction, even total crop failure.For controlling the popular of soil disease, a large amount of chemical pesticides that use in planting process, further having aggravated rhizosphere soil microorganism group changes, also bring simultaneously the in a large number accumulation in soil and plant of inorganic chemistry agricultural chemicals, the crop Pesticide Residues is high, affect the quality of special product crop, reduced the market competitiveness.Therefore, develop a kind of new-type fertilizer that can effectively control soil disease, when reducing the chemical pesticide usage quantity, can improve the yield and quality of some special product crops, this is very necessary and urgent in the production of agricultural special product.
Summary of the invention
The object of the invention is a kind of solid union actinomycetes bacterial manure and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of solid union actinomycetes bacterial manure: by weight percentage, 20% grey violet streptomycete T005(
Streptomyces violascensT005, deposit number CCTCC NO:M 2012185), 20% Streptomycesalbidoflhaving T006(
Streptomyces albidoflavusT006, deposit number CCTCC NO:M 2012186), ground, 20% Shandong streptomycete T009(
Streptomyces rutgersensisT009, deposit number CCTCC NO:M 2012187), 20% Gaoyang streptomycete T011 (
Streptomyces koyangensisT011, deposit number CCTCC NO:M 2012188) and 20% gelatin sample streptomycete T013 (
Streptomyces gelaticusT013, deposit number CCTCC NO:M 2012189) fermented product is mixed to be formed.
[0005]The preparation method of solid union actinomycetes bacterial manure: with grey violet streptomycete T005(
Streptomyces violascensT005, deposit number CCTCC NO:M 2012185), Streptomycesalbidoflhaving T006(
Streptomyces albidoflavusT006, deposit number CCTCC NO:M 2012186), ground, Shandong streptomycete T009(
Streptomyces rutgersensisT009, deposit number CCTCC NO:M 2012187), Gaoyang streptomycete T011 (
Streptomyces koyangensisT011, deposit number CCTCC NO:M 2012188) and gelatin sample streptomycete T013 (
Streptomyces gelaticusT013, deposit number CCTCC NO:M 2012189) etc. bacterial strain carries out respectively seed culture in liquid nutrient medium separately, be cultured to logarithmic phase, the solid fermentation substratum that is seeded to separately by 10% inoculum size respectively carries out fermentation culture, the solid culture that is cultured to the spore ripening stage is evenly formed viable count 〉=26.8 * 10 after mixing by equal proportion is mixed together with substratum
8Individual/gram.
[0006]Grey violet streptomycete T005(
Streptomyces violascensT005, deposit number CCTCC NO:M 2012185): grey violet streptomycete T005 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10;
Streptomycesalbidoflhaving T006(
Streptomyces albidoflavusT006, deposit number CCTCC NO:M 2012186): Streptomycesalbidoflhaving T006 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10;
Ground, Shandong streptomycete T009(
Streptomyces rutgersensisT009, deposit number CCTCC NO:M 2012187): ground, Shandong streptomycete T009 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10;
Gaoyang streptomycete T011 (
Streptomyces koyangensisT011, deposit number CCTCC NO:M 2012188): Gaoyang streptomycete T011 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10;
Gelatin sample streptomycete T013 (
Streptomyces gelaticusT013, deposit number CCTCC NO:M 2012189): gelatin sample streptomycete T013 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10.
The present invention has advantages of:
The employed bacterial classification of fertilizer of the present invention is the streptomyces species with broad spectrum antibiotic activity and good cultivation that obtains from soil, animal excrement, they are made bacterial manure, return soil, can increase the antagonistic microbe of soil, the control pathogenic micro-organism, improve and recovery Ecological Distribution of Soil Microorganisms balance, safety is residual without farming, meets the direction of building the ecological agriculture; With the compound bacterial fertilizer that 5 kinds of different streptomycetes are made, production technique is easy, is easy to accomplish scale production; This compound bacterial fertilizer is difficult for causing pathogenic bacteria develop immunity to drugs (resistance, especially cross-resistance are one of serious problems of agricultural chemicals, medical applications); Prevent and treat soil-borne disease with this compound bacterial fertilizer, environmental protection safety, prevention effect is good, and cost is low, and is easy to use.
[0008]Compound streptomycete bacterial manure of the present invention is applicable to all perennial agricultural produce plants, has the crop of continuous cropping obstacle, plants in earlier stage and all can use mid-term, can strengthen soil fertility, reduces the generation of soil-borne disease, improves crop quality, increases output.Can improve after the mid-term fertilising seedling stage and planting season plant survival rate and disease resistance ability.Take pseudo-ginseng as example, the survival rate in continuous cropping seedling stage improves approximately 60%.Establiss phase survival rate improves 70%.
Embodiment
Embodiment
Solid union actinomycetes bacterial manure: by weight percentage, 20% grey violet streptomycete T005(
Streptomyces violascensT005, deposit number CCTCC NO:M 2012185, preservation date are on May 29th, 2012), 20% Streptomycesalbidoflhaving T006(
Streptomyces albidoflavusT006, deposit number CCTCC NO:M 2012186, preservation date are on May 29th, 2012), ground, 20% Shandong streptomycete T009(
Streptomyces rutgersensisT009, deposit number CCTCC NO:M 2012187, preservation date are on May 29th, 2012), 20% Gaoyang streptomycete T011 (
Streptomyces koyangensisT011, deposit number CCTCC NO:M 2012188, preservation date are on May 29th, 2012) and 20% gelatin sample streptomycete T013 (
Streptomyces gelaticusT013, deposit number CCTCC NO:M 2012189, preservation date are on May 29th, 2012) mixed the forming of fermented product.
[0010]The preparation method of solid union actinomycetes bacterial manure:
Grey violet streptomycete T005: grey violet streptomycete T005 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10.Described grey violet streptomycete (
Streptomyces violascens)T005 separates the fresh excreta from elephant, is kept at Chinese Typical Representative culture collection center, is numbered CCTCC NO:M 2012185.
[0011]Streptomycesalbidoflhaving T006: Streptomycesalbidoflhaving T006 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10.Described Streptomycesalbidoflhaving
Streptomyces albidoflavusT006 separates the fresh excreta from elephant, is kept at Chinese Typical Representative culture collection center, is numbered CCTCC NO:M 2012186.
[0012]Ground, Shandong streptomycete T009: ground, Shandong streptomycete T009 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10.Ground, described Shandong streptomycete
Streptomyces rutgersensisT009 separates the fresh excreta from elk, is kept at Chinese Typical Representative culture collection center, is numbered CCTCC NO:M 2012187.
Gaoyang streptomycete T011: Gaoyang streptomycete T011 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10.Described Gaoyang streptomycete
Streptomyces koyangensisT011 separates the fresh excreta from Rhinopithecus bieti, is kept at Chinese Typical Representative culture collection center, numbering CCTCC NO:M 2012188.
[0014]Gelatin sample streptomycete T013: gelatin sample streptomycete T013 is inoculated in the solid medium by 20%, in 28-30 ℃ of fermentation 10 days, stand-by; Solid medium is by weight proportion: wheat bran 7: rice bran 3: water 10.Described gelatin sample streptomycete
Streptomyces gelaticusThe fresh excreta of T013 Fen Li Zi Shu antelope is kept at Chinese Typical Representative culture collection center, numbering CCTCC NO:M 2012189.
[0015]After equal proportion mixes by weight with the solid matter after the above-mentioned cultivation, viable count 〉=26.8 * 10
8Individual/gram, namely get the compound actinomycetes bacterial manure of solid.
The pseudo-ginseng of management and cultivation with above-mentioned preparation gained solid union actinomycetes bacterial manure, carries out rhizosphere fertilization with 30 g/ms as subjects routinely in the booth, the control group that design is not used, 3 repetitions of each treatment group, each repeats 66 square metres, the survival rate of pseudo-ginseng in the determination test phase.Test-results shows, the survival rate of control group is 12%, and the survival rate of test group is 83%, has improved 71%.
Claims (3)
1. solid union actinomycetes bacterial manure is characterized in that the bacterial strain of its composition is: the grey violet streptomycete (
Streptomyces violascens, T 005=CCTCC M 2012185), Streptomycesalbidoflhaving (
Streptomyces albidoflavus, T 006=CCTCC M 2012186), Shandong ground streptomycete (
Streptomyces rutgersensis, T 009=CCTCC M 2012187), the Gaoyang streptomycete (
Streptomyces koyangensis, T 011=CCTCC M 2012188) and 20% gelatin sample streptomycete (
Streptomyces gelaticus, T 013=CCTCC M 2012189).
2. solid union actinomycetes bacterial manure according to claim 1, it is characterized in that: described actinomycetes all separate the animal fresh excreta of putting in a suitable place to breed from the Safari Park, wherein, the grey violet streptomycete
Streptomyces violascensT005 separates fresh excreta, the Streptomycesalbidoflhaving from elephant
Streptomyces albidoflavusT 006 separates fresh excreta, ground, the Shandong streptomycete from elephant
Streptomyces rutgersensisFresh excreta, Gaoyang streptomycete that T 009 separates from elk
Streptomyces koyangensisFresh excreta, gelatin sample streptomycete that T 011 separates from Rhinopithecus bieti
Streptomyces gelaticusThe fresh excreta of T 013 Fen Li Zi Shu antelope.
3. the actinomycetic preparation method of solid union according to claim 1, it is characterized in that: described actinomycetes carry out respectively seed culture in liquid nutrient medium separately, be cultured to logarithmic phase, the solid fermentation substratum that is seeded to separately by 10% inoculum size respectively carries out fermentation culture, the solid culture that is cultured to the spore ripening stage is evenly formed viable count 〉=26.8 * 10 after mixing by equal proportion is mixed together with substratum
8Individual/gram.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184184A (en) * | 2013-04-24 | 2013-07-03 | 牛赡光 | Streptomyces albidoflavus and applications thereof |
| CN111139205A (en) * | 2020-02-11 | 2020-05-12 | 东北农业大学 | Streptomyces and microbial fertilizer thereof, preparation method and application of microbial fertilizer |
| CN115093295A (en) * | 2022-07-05 | 2022-09-23 | 云南大学 | Streptomyces lividans bacterial manure and preparation method thereof |
| CN115583847A (en) * | 2022-06-29 | 2023-01-10 | 云南大学 | Streptomyces rubiae bacterial fertilizer and preparation method thereof |
-
2012
- 2012-10-24 CN CN2012104078699A patent/CN103044096A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103184184A (en) * | 2013-04-24 | 2013-07-03 | 牛赡光 | Streptomyces albidoflavus and applications thereof |
| CN103184184B (en) * | 2013-04-24 | 2014-11-19 | 牛赡光 | Streptomyces albidoflavus and applications thereof |
| CN111139205A (en) * | 2020-02-11 | 2020-05-12 | 东北农业大学 | Streptomyces and microbial fertilizer thereof, preparation method and application of microbial fertilizer |
| CN115583847A (en) * | 2022-06-29 | 2023-01-10 | 云南大学 | Streptomyces rubiae bacterial fertilizer and preparation method thereof |
| CN115093295A (en) * | 2022-07-05 | 2022-09-23 | 云南大学 | Streptomyces lividans bacterial manure and preparation method thereof |
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Application publication date: 20130417 |