CN105052471A - A method for cultivating tobacco disease-resistant strong seedlings by utilizing beneficial biological bacteria - Google Patents
A method for cultivating tobacco disease-resistant strong seedlings by utilizing beneficial biological bacteria Download PDFInfo
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- CN105052471A CN105052471A CN201510466910.3A CN201510466910A CN105052471A CN 105052471 A CN105052471 A CN 105052471A CN 201510466910 A CN201510466910 A CN 201510466910A CN 105052471 A CN105052471 A CN 105052471A
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- bacillus subtilis
- seedling
- disease resistance
- tobacco
- strong sprout
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention belongs to the field of plant seedling cultivating and specifically relates to a method for cultivating tobacco disease-resistant strong seedlings by utilizing beneficial biological bacteria. The method for cultivating tobacco disease-resistant strong seedlings comprises four steps: seedling cultivation preparation, bacillus subtilis liquid preparation, bacillus subtilis liquid spraying, and seedling cultivation management. The bacillus subtilis liquid comprises the following components in part by weight: 1 part of bacillus subtilis raw powders, 4-6 parts of brown sugars and 20-25 parts of water, wherein the weight of the bacillus subtilis raw powders accounts for 0.2% of the weight of a matrix. The method is used for cultivating tobacco seedlings by utilizing the beneficial biological bacteria; the tobacco seedlings and bacillus subtilis are symbiotic in the nursery stage; and the seedling cultivation test where the bacillus subtilis is added shows that, in the seedling cultivating process, root systems of the tobacco seedlings are allowed to be stronger, the tobacco seedlings are allowed to be healthier and have disease resistance by adding the bacillus subtilis.
Description
Technical field
The invention belongs to botanical seedling culturing field, particularly a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout.
Background technology
Tobacco rhizome class disease (as: balck shank, root rot) is the important disease of China's tobacco planting, and long-term occurring area accounts for 6% of the gross area, and loss, more than 300,000,000 yuan, often causes total crop failure in some grave illness district, the stable development of extreme influence tobacco.At present, control tobacco rhizome class disease is taken as the leading factor with chemicals, but large area drug cost higher (be not every block ground or all occur every year) in advance, and it is residual to increase agriculture, but once illness shows, medication control efficiency is not satisfactory again.
Biological control is the direction of tobacco plant protection, and bacillus subtilis is by surely growing in plant rhizosphere, body surface or body, and with the nutrition around pathogen competitive plant, secretion antibacterial material is to suppress growth of pathogenic bacteria, and inducing plant system of defense resists pathogen invasion simultaneously,
Thus reach the object of biological and ecological methods to prevent plant disease, pests, and erosion, meanwhile, bacillus subtilis has the function promoting plant growth, and many local trials utilize bacillus subtilis to prevent and treat tobacco rhizome class disease now.Its general method is the reagent utilizing this type of bacterium to prepare, and spreads fertilizer over the fields in tobacco growth cave when cigarette transplantation of seedlings.The method defect is: (1) cost is high, and (2) recruitment is many, (3) cannot test organisms bacterium active and cause effect unstable.
China's tobacco planting is throughout the year at about 1,800 ten thousand mu, and cultivation method all adopts the mode of seedling raising and transplanting, and therefore, the quality of Quality of Seedlings, plays an important role to tobacco grown in field.At present, the nursery target of proposition is anosis strong sprout, but the nursery overwhelming majority's is inorganic fertilizer, there is root system more weak, the problem of disease resistance difference.
Based on the feature of biological control theory and tobacco seedling, develop a kind of method utilizing beneficial microbe to cultivate tobacco disease resistance strong sprout very necessary.
Summary of the invention
More weak for the tobacco seedling root system existed in existing tobacco seedling technology, resistance against diseases is weak and biological tobacco prevention and control field to there is cost high, recruitment is many, cannot the defect of test organisms bacterium activity, the present invention adds bacillus subtilis in tobacco seedling process, allow cigarette seedling and bacillus subtilis bacterium carry out symbiosis in seedling stage, object is to propose a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout.
Technical scheme of the present invention is as follows:
Utilize beneficial organism bacterium to cultivate the method in tobacco disease resistance strong sprout, the method using Bacillus subtilis in described cultivation tobacco disease resistance strong sprout, makes cigarette seedling and bacillus subtilis in nursery stage symbiosis.
Further, the method in described cultivation tobacco disease resistance strong sprout comprises the steps:
(1) nursery prepares: by loading matrix in seedling-cultivating tray after sterilization, be sown into tobacco seed, seedling-cultivating tray put into nursery pond containing nutrient solution;
(2) prepare bacillus subtilis bacterium liquid: take the former powder of bacillus subtilis, brown sugar and water and be placed in bucket and stir, then leave standstill 3-4 hour, obtain bacillus subtilis bacterium liquid;
(3) bacillus subtilis bacterium liquid is sprayed: get the bacterium liquid that step (2) prepares and be placed in clean sprayer, the seedling-cultivating tray sowed in nursery pond is evenly sprayed;
(4) seedling management: seedling raising manners carries out routinely.
Further, described bacillus subtilis bacterium liquid comprises the composition of following weight proportion: 1 part, the former powder of described bacillus subtilis, described brown sugar 4-6 part, described water 20-25 part.
Further, described preparation bacillus subtilis bacterium liquid step mixing speed is 100 ~ 200rmp/min.
Further, the weight of the former powder of described bacillus subtilis accounts for 0.2% of described matrix weight.
Further, every gram of former powder of described bacillus subtilis contains 100-600 hundred million bacillus subtilises.
Further, described matrix is not blending or the conventional substrate spraying bactericide.
Further, it is characterized in that: described conventional seedbed system mode is floating seedlings or moistening nursery.
Beneficial effect of the present invention:
The present invention adds bacillus subtilis at seedling raising process, allows cigarette seedling and bacillus subtilis carry out symbiosis in seedling stage.By seedling growth test, the effect that seedling raising process adds bacillus subtilis is studied, result shows that seedling raising process adds bacillus subtilis and can obtain following beneficial effect: (1) tobacco root system is more flourishing: during seedling, the tobacco root system fresh weight adding bacterial classification reaches 3.03 grams, contrast is 1.63 grams, is 1.86 times of contrast.(2) cigarette seedling is more healthy and stronger: during seedling, and the fresh and dried ratio of cigarette seedling cauline leaf adding bacterial classification is 0.1087, exceeds 16% than contrast.(3) bacillus subtilis obtains survival and breeding under seedling raising environment, reach higher, be enough to disease-resistant level: according to detection, during seedling, in every gram of matrix, viable bacteria amount reaches 1.6 × 10
7cfu, and contrast is zero.
Accompanying drawing explanation
Fig. 1 is the schematic diagram utilizing beneficial organism bacterium to cultivate tobacco disease resistance step in strong sprout.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is explained in further detail.Should be appreciated that specific embodiment described herein only for explaining the present invention, being not intended to limit the present invention.
On the contrary, the present invention is contained any by the substituting of making on marrow of the present invention and scope of defining of claim, amendment, equivalent method and scheme.Further, in order to make the public have a better understanding to the present invention, in hereafter details of the present invention being described, detailedly describe some specific detail sections.Do not have the description of these detail sections can understand the present invention completely for a person skilled in the art yet.
embodiment 1
Arrange two groups of cigarette seedling culture experiments, a processed group and a control group, described processed group is being taked floating seedlings mode basis with the addition of bacillus subtilis, and control group adopts conventional floating seedlings mode.
Conventional standard seedling-cultivating tray (200 strain) is filled up conventional substrate (1.5kg), and sowing subsequently covers the nursery pond put into containing conventional nutrient liquid.
Take 3 grams of former powder of (by 0.2% of matrix weight) bacillus subtilis (every gram containing 20,000,000,000 bacillus subtilises), 15 grams of brown sugar, 0.6kg water, above-mentioned raw materials is put into a keg, stir, leave standstill 4 hours.
Processed group: get the clear liquid of bacterium liquid prepared by above-mentioned steps in the sprayer of cleaning, is evenly sprayed onto described seedling-cultivating tray (sowing covering) surface.
Control group: the clear water spraying equivalent.
Seedling management routinely floating seedlings mode carries out.
Investigate into the biological character of seedling stage (after planting 53 days) cigarette seedling, and monitor bacillus subtilis number of viable.
Cigarette seedling becomes the biological character in seedling stage to show, the fresh dry weight of root system of processed group, apparently higher than control group, shows that the tobacco root system adding bacillus subtilis process is more flourishing.The fresh and dried control group frequently of cauline leaf of processed group exceeds 16%, and show that the cigarette seedling dry matter content adding bacillus subtilis process is more abundant, cigarette seedling is more healthy and stronger.
The impact of different disposal method on cigarette seedling biological character is as shown in the table:
Cigarette seedling becomes seedling stage, carries out living stems to the seedling medium of processed group and control group, and through Dohanykutato Intezet of academy of agricultural sciences of Henan Province, the qualification of plant protection chamber microbiological detects, and detects that in processed group matrix, bacillus subtilis active amt is every gram 1.6 × 10
7cfu, and the bacillus subtilis of control test is zero.In processed group matrix, Bacillus subtilis number is enough to antagonism harmful levels of pathogens.
Above result of the test shows can to survive and breed in the environment of bacillus subtilis at tobacco seedling, can symbiosis promote tobacco seedling growth with cigarette seedling.
embodiment 2
The present embodiment is identical with above-described embodiment part, and only difference is: conventional standard seedling-cultivating tray (200 strain) is filled up conventional substrate (1.5kg), and sowing subsequently covers the nursery pond put into containing conventional nutrient liquid.
Take 3 grams of former powder of (by 0.2% of matrix weight) bacillus subtilis (every gram of 20,000,000,000 bacillus subtilises), 16 grams of brown sugar, 0.65kg water, above-mentioned raw materials is put into a keg, different from embodiment 1, the present embodiment stirs 3 hours with the rotating speed of 100r/min, then leaves standstill 1 hour.
Processed group: get bacterium liquid prepared by above-mentioned steps in the sprayer of cleaning, is evenly sprayed onto described seedling-cultivating tray (sowing covering) surface.
Seedling management routinely floating seedlings mode carries out.
The biological character of investigation cigarette seedling, and be detected as the active amt of bacillus subtilis in seedling stage (after planting 53 days) its matrix.
Result shows, in the step of preparation bacillus subtilis bacterium liquid, mixing speed can affect the activation effect of bacillus subtilis, when in mixing speed 200r/min, along with the increase of mixing speed, in bacterium liquid, dissolved oxygen content is higher, better to the metabolic effects of brown sugar, actication of culture effect is better, is goodly conducive to the propagation of bacillus subtilis in matrix.Cultivate the cigarette seedling obtained according to the present embodiment method, well developed root system, cigarette seedling are healthy and strong.When cigarette seedling is in into seedling stage, detect the active amt of bacillus subtilis in its matrix, be every gram 1.7 × 10
7cfu, this shows that bacillus subtilis can survive, breed in the environment of tobacco seedling, and can with the symbiosis of cigarette seedling, promote the growth of cigarette seedling.
Claims (8)
1. utilize beneficial organism bacterium to cultivate the method in tobacco disease resistance strong sprout, it is characterized in that: the method using Bacillus subtilis in described cultivation tobacco disease resistance strong sprout, makes cigarette seedling and bacillus subtilis in nursery stage symbiosis.
2. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 1, is characterized in that: the method in described cultivation tobacco disease resistance strong sprout comprises the steps:
(1) nursery prepares: by loading matrix in seedling-cultivating tray after sterilization, be sown into tobacco seed, seedling-cultivating tray put into nursery pond containing nutrient solution;
(2) prepare bacillus subtilis bacterium liquid: take the former powder of bacillus subtilis, brown sugar and water and be placed in bucket and stir, then leave standstill 3-4 hour, obtain bacillus subtilis bacterium liquid;
(3) bacillus subtilis bacterium liquid is sprayed: get the bacterium liquid that step (2) prepares and be placed in clean sprayer, the seedling-cultivating tray sowed in nursery pond is evenly sprayed;
(4) seedling management: seedling raising manners carries out routinely.
3. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 2, it is characterized in that: described bacillus subtilis bacterium liquid comprises the composition of following weight proportion: 1 part, the former powder of described bacillus subtilis, described brown sugar 4-6 part, described water 20-25 part.
4. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 2, is characterized in that: the weight of the former powder of described bacillus subtilis accounts for 0.2% of described matrix weight.
5. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 2, is characterized in that: described preparation bacillus subtilis bacterium liquid step mixing speed is 100 ~ 200rmp/min.
6. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 3, is characterized in that: every gram of former powder of described bacillus subtilis contains 100-600 hundred million bacillus subtilises.
7. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 2, is characterized in that: described matrix is not blending or the conventional substrate spraying bactericide.
8. a kind of method utilizing beneficial organism bacterium to cultivate tobacco disease resistance strong sprout according to claim 2, is characterized in that: described conventional seedbed system mode is floating seedlings or moistening nursery.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108811577A (en) * | 2018-07-16 | 2018-11-16 | 江苏丘陵地区镇江农业科学研究所 | A method of reducing strawberry soil cause of disease bacterial content |
| CN108935039A (en) * | 2018-06-29 | 2018-12-07 | 河南省农业科学院烟草研究所 | A kind of tobacco floating seed rearing promoting root growth strong seedling culture method |
| CN109673343A (en) * | 2019-03-07 | 2019-04-26 | 贵州省植物保护研究所 | A kind of integrated approach of prevention and control Cruciferae clubroot |
| CN110511891A (en) * | 2019-08-26 | 2019-11-29 | 河南省烟草公司许昌市公司 | A kind of and anti-tobacco root rot and the microbial bacterial agent of balck shank and its preparation method and application |
| CN113349227A (en) * | 2021-05-12 | 2021-09-07 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935039A (en) * | 2018-06-29 | 2018-12-07 | 河南省农业科学院烟草研究所 | A kind of tobacco floating seed rearing promoting root growth strong seedling culture method |
| CN108811577A (en) * | 2018-07-16 | 2018-11-16 | 江苏丘陵地区镇江农业科学研究所 | A method of reducing strawberry soil cause of disease bacterial content |
| CN108811577B (en) * | 2018-07-16 | 2021-04-23 | 江苏丘陵地区镇江农业科学研究所 | Method for reducing content of pathogenic bacteria in strawberry soil |
| CN109673343A (en) * | 2019-03-07 | 2019-04-26 | 贵州省植物保护研究所 | A kind of integrated approach of prevention and control Cruciferae clubroot |
| CN110511891A (en) * | 2019-08-26 | 2019-11-29 | 河南省烟草公司许昌市公司 | A kind of and anti-tobacco root rot and the microbial bacterial agent of balck shank and its preparation method and application |
| CN113349227A (en) * | 2021-05-12 | 2021-09-07 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
| CN113349227B (en) * | 2021-05-12 | 2022-06-17 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
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Application publication date: 20151118 |