CN103007053A - Dendrobium officinale compound preparation as well as preparation method and application thereof - Google Patents
Dendrobium officinale compound preparation as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a dendrobium officinale compound preparation as well as a preparation method and an application thereof. The dendrobium officinale compound preparation comprises the following raw materials: 2-60 g of dendrobium officinale, 2-60 g of medlar, 1-50 g of American ginseng and 1-50 g of momordica grosvenori; the dendrobium officinale, the medlar and the momordica grosvenori are extracted by water; and the American ginseng is extracted by ethanol with the concentration of 40-60%. The weight of obtained dendrobium officinale extractive powder is 0.4-12 g, the weight of medlar extractive powder is 0.4-12 g, the weight of American ginseng extractive powder is 0.2-10 g and the weight of momordica grosvenori extractive powder is 0.2-10 g. The dendrobium officinale compound preparation further contains an auxiliary material. The invention further provides the preparation method and the application of the dendrobium officinale compound preparation; a function test shows that the prepared dendrobium officinale compound preparation has health-care functions of enhancing immunity and relieving physical fatigue; and the dendrobium officinale compound preparation can be widely applied to heath care of people with low immunity, fatigable people, and middle aged and elderly people.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof and purposes, particularly, relate to a kind of Herba Dendrobii compound preparation and preparation method thereof and purposes.
Background technology
In recent years, development and growth in the living standard along with science and technology, compatriots' average life constantly prolongs, aging is stepped in many cities gradually, in order to improve the quality of living, strengthen life and render a service, rationally use illness prevention and fitness health product oneself become gradually people's a kind of conscious requirement, the research and development in this field also more and more comes into one's own.
Herba Dendrobii is the stem of orchid Herba Dendrobii, and its medicinal beginning is stated from Shennong's Herbal and is listed in top grade.Motherland's medical science thinks and enter stomach, kidney channel by Herba Dendrobii slightly sweet flavor, little salty, cold in nature, and is nontoxic, has the effect of the stomach reinforcing that promotes the production of body fluid, clearing away heat and nourishing YIN.Be applicable to that calentura later stage cloudy Tianjin consumption is hindered and deficiency-heat is not moved back, irritated thirster.But the treating the liver deficiency of the kidney yin is replenishing YIN and removing heat, the key medicine of reinforcing stomach reg fluid.Li Shizhen (1518-1593 A.D.) claims it that the siddhi special effect of " reinforcing YIN-essence benefit is smart, and thick the intestines and stomach is never sufficient in the benefit, flat gastric qi, longue meat, sedate is made light of one's life by commiting suicide and prolonged life except frightened " is arranged in Compendium of Material Medica.The successive dynasties peculiar effect of medicine and pharmacology works Herba Dendrobii has clear and definite record, thinks that it is the superfine product of YIN nourishing tonify deficiency.Modern medicine and pharmacology studies have shown that active component dendrobium polysaccharide contained in the Herba Dendrobii, has immune dual regulation, to the immunocompromised person, can resist by regulating function of immune system the invasion and attack of disease; To the immunologic function hyperthyroid, by regulating function of immune system, recover its normal level.
Fructus Lycii claims again red ear pendant, is that the ripe son of Solanaceae undershrub Fructus Lycii is real, and it is edible both to have can be used as nut, is again the distinguished Chinese medicine material of effect simply, usually is used as nourishing and takes good care of and antidotal good medicine.At all times pharmacology and clinical research proves that all Fructus Lycii truly has and prevents and cures diseases, the effect of life lengthening.
The Radix Panacis Quinquefolii sweet and slightly bitter taste, cool in nature, enter cardiopulmonary kidney three warps, lung benefiting is cloudy, clear asthenic fire, promoting the production of body fluid to quench thirst, supplementary Amplifications of the Compendium of Materia Medica are called it " relieving restlessness is tired for tonifying the lung pathogenic fire reducing, born fluid, and are empty and have fiery person suitable." so the people of all hyperactivity of fire caused by deficiency of YIN chronic cough, every with Radix Panacis Quinquefolii then the flat fire of gas hold back, cough is gradually flat.
The Fructus Momordicae formal name used at school is that the Cucurbitaceae Fructus Momordicae belongs to the perennial dry fruit of climbing up by holding on to the liana Fructus Momordicae, and cool in nature, sweet in the mouth, nontoxic is returned lung, large intestine channel, and the effect of nourishing the lung to arrest cough, removing heat from blood laxation is arranged.
Summary of the invention
The purpose of this invention is to provide a kind of Herba Dendrobii compound preparation for health care and preparation method thereof and purposes, this Herba Dendrobii compound preparation has enhancing immunity, the health care of alleviating physical fatigue.
In order to achieve the above object, the invention provides a kind of Herba Dendrobii compound preparation, wherein, this Herba Dendrobii compound preparation comprises following raw material: Herba Dendrobii 2 ~ 60g, Fructus Lycii 2 ~ 60g, Radix Panacis Quinquefolii 1 ~ 50g, Fructus Momordicae 1 ~ 50g.Preferred each raw material weight proportioning is Herba Dendrobii 20g, Fructus Lycii 20g, Radix Panacis Quinquefolii 10g, Fructus Momordicae 10g.This Herba Dendrobii compound preparation is by Herba Dendrobii, Fructus Lycii, Radix Panacis Quinquefolii and Fructus Momordicae synergism, and the crude polysaccharides that wherein contains, American ginseng total saponins etc. have immunomodulating, anti-fatigue effect.
Described Herba Dendrobii, Fructus Lycii and Fructus Momordicae all carry out water extraction, and described Radix Panacis Quinquefolii carries out 40% ~ 60% ethanol extraction.Effective ingredient is not quite similar in the contained raw material because the Herba Dendrobii compound preparation is write out a prescription, the contained functional component of Herba Dendrobii is mainly dendrobium polysaccharide, the functional component of Fructus Lycii is lycium barbarum polysaccharide, and Fructus Momordicae strengthens the effective site of immunity at its water extraction position, therefore these three kinds of raw materials adopt water extraction, and the functional component of Radix Panacis Quinquefolii is except water soluble polysaccharide, and also having American ginseng total saponins is liposoluble constituent, so adopt ethanol extraction.
Above-mentioned Herba Dendrobii compound preparation wherein, also further includes adjuvant in the described Herba Dendrobii compound preparation; Described adjuvant comprises any one or a few the combination in 2 ~ 100g glucose, 2 ~ 100g lactose, 2 ~ 100g sucrose, 2 ~ 100g starch, 2 ~ 100g dextrin, 2 ~ 100g microcrystalline Cellulose, 0.2 ~ 10g magnesium stearate, 2 ~ 100g Mel and the 0.2 ~ 10g Pulvis Talci.
Above-mentioned Herba Dendrobii compound preparation, wherein, the dosage form of described Herba Dendrobii compound preparation comprises tablet, capsule, granule, pill, powder, oral liquid, medicated wine or unguentum.The preparation of tablet, capsule, pill, granule, powder: get extract mixing powder and proper auxiliary materials mix homogeneously that step 5 makes, make according to corresponding dosage form common process processing.The preparation of oral liquid, medicated wine, unguentum: get that extract mixing powder that step 5 makes adds water according to common process or ethanol dissolves, add suitable adjuvant mix homogeneously, make by corresponding dosage form common process processing.
The present invention also provides a kind of preparation method of Herba Dendrobii compound preparation described above, and wherein, described preparation method comprises: step 1, choose qualified Herba Dendrobii raw material, be cut into the segment that length is 5 ~ 8 mm at microtome, add the twelvefold water gaging and decoct each 3 hours three times, collecting decoction, filter, filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of crushed after being dried, obtain described Herba Dendrobii extract powder, for subsequent use; Step 2 is chosen qualified Fructus Lycii raw material, and coarse pulverization on coarse-cracking machine adds the twelvefold water gaging and decocts three times, each 3 hours, collecting decoction filtered, and filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of dryings, again pulverize, obtain described Fructus Lycii extraction powder, for subsequent use; Step 3, choose qualified Radix Panacis Quinquefolii raw material, coarse pulverization on coarse-cracking machine adds 6 times of amount 50% alcohol heating reflux and extracts three times, each 3 hours, merge extractive liquid,, Recycled ethanol, filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of dryings, again pulverize, obtain described Radix Panacis Quinquefolii extract powder, for subsequent use; Step 4 is chosen qualified Fructus Momordicae raw material, and coarse pulverization on coarse-cracking machine adds the twelvefold water gaging and decocts three times, each 3 hours, collecting decoction filtered, and filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of dryings, again pulverize, obtain described Fructus Momordicae extract powder, for subsequent use; Step 5, with the Herba Dendrobii extract powder of step 1 to step 4 gained, Fructus Lycii extraction powder, Radix Panacis Quinquefolii extract powder and Fructus Momordicae extract powder mix homogeneously are processed into preparation.
The preparation method of above-mentioned Herba Dendrobii compound preparation, wherein, the Herba Dendrobii extract powder described in the step 5 is 0.4 ~ 12g, and Fructus Lycii extraction powder is 0.4 ~ 12g, and the Radix Panacis Quinquefolii extract powder is 0.2 ~ 10g, and the Fructus Momordicae extract powder is 0.2 ~ 10g.
The preparation method of above-mentioned Herba Dendrobii compound preparation wherein, comprises further also in the described step 5 that adding the adjuvant post-treatment becomes preparation.Described adjuvant comprises any one or a few the combination in 2 ~ 100g glucose, 2 ~ 100g lactose, 2 ~ 100g sucrose, 2 ~ 100g starch, 2 ~ 100g dextrin, 2 ~ 100g microcrystalline Cellulose, 0.2 ~ 10g magnesium stearate, 2 ~ 100g Mel and the 0.2 ~ 10g Pulvis Talci.
The present invention also provides the purposes of a kind of above-mentioned Herba Dendrobii compound preparation for the preparation of health care medicine, can enhancing immunity, alleviating physical fatigue.
Herba Dendrobii compound preparation provided by the invention and preparation method thereof with the advantage of purposes is, Herba Dendrobii Yin-nourishing and body fluid promoting is wherein joined the Fructus Lycii invigorating QI and tranquilization, joins the Radix Panacis Quinquefolii boosting qi and nourishing yin, joins the Fructus Momordicae lung benefiting and promotes the production of body fluid, and closes and uses it.The body that both had been suitable for the vital essence deficiency is suitable for again the people of YIN fluid deficiency, is used for deficiency of both the lung and kidney, deficiency of both vital energy and Yin, fatigue and weakness, lumbocrural pain, the few expectorant of cough, dry mouth and throat, deficient pulse weak person.This compound preparation is applicable to the immunocompromised person, the fatiguability person, and middle-aged and elderly people and be in all kinds of crowds of sub-health state also can be used for the rehabilitation behind the cancer patient chemicotherapy.
The specific embodiment
Below the specific embodiment of the present invention is further described.
Embodiment 1
The agent of present embodiment Herba Dendrobii compound granular, its crude drug proportioning is:
Principal agent: Herba Dendrobii 20g, Fructus Lycii 20g, Radix Panacis Quinquefolii 10g, Fructus Momordicae 10g; Adjuvant: dextrin 20g.
Be prepared according to following concrete mode:
(1) Herba Dendrobii is cut into 5 ~ 8 mm left and right sides thin segments, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(2) Fructus Lycii powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(3) Radix Panacis Quinquefolii powder is broken into coarse powder, and add 6 times of amount 50% alcohol heating reflux and extract three times, each 3 hours, merge extractive liquid,, Recycled ethanol, 70～80 ℃ of concentrating under reduced pressure, 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(4) mangosteen powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(5) get Herba Dendrobii extract fine powder 4g, wolfberry fruit extract fine powder 4g, Radix Panacis Quinquefolii extract fine powder 4g and Fructus Momordicae extract fine powder 4g mix homogeneously.
With obtained extract fine powder and the dextrine powder mix homogeneously of crossing 80 mesh sieves, spraying adds the ethanol soft material processed that is equivalent to described mixed material weight 5%, and 20 mesh sieves are granulated, drying, and granulate gets granule.
Present embodiment compound dendrobium officinale granule, the adult takes twice every day, each 2g.
Embodiment 2
The agent of present embodiment Herba Dendrobii compound granular, its crude drug proportioning is:
Principal agent: Herba Dendrobii 20g, Fructus Lycii 15g, Radix Panacis Quinquefolii 15g, Fructus Momordicae 10g; Adjuvant: dextrin 20g.
Be prepared according to following concrete mode:
(1) Herba Dendrobii is cut into 5 ~ 8 mm left and right sides thin segments, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(2) Fructus Lycii powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(3) Radix Panacis Quinquefolii powder is broken into coarse powder, and add 6 times of amount 50% alcohol heating reflux and extract three times, each 3 hours, merge extractive liquid,, Recycled ethanol, 70～80 ℃ of concentrating under reduced pressure, 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(4) mangosteen powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(5) get Herba Dendrobii extract fine powder 4g, wolfberry fruit extract fine powder 3g, Radix Panacis Quinquefolii extract fine powder 5g and Fructus Momordicae extract fine powder 4g mix homogeneously.
With obtained extract fine powder and the dextrine powder mix homogeneously of crossing 80 mesh sieves, spraying adds the ethanol soft material processed that is equivalent to described mixed material weight 5%, and 20 mesh sieves are granulated, drying, and granulate gets granule.
Present embodiment compound dendrobium officinale granule, the adult takes twice every day, each 2g.
Embodiment 3
Present embodiment Herba Dendrobii compound tablet, its crude drug proportioning is:
Principal agent: Herba Dendrobii 40g, Fructus Lycii 30g, Radix Panacis Quinquefolii 25g, Fructus Momordicae 20g; Adjuvant: dextrin 10g, microcrystalline Cellulose 10g, magnesium stearate: 0.5g.
Preparation method:
(1) Herba Dendrobii is cut into 5 ~ 8 mm left and right sides thin segments, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use;
(2) Fructus Lycii powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(3) Radix Panacis Quinquefolii powder is broken into coarse powder, and add 6 times of amount 40% alcohol heating reflux and extract three times, each 3 hours, merge extractive liquid,, Recycled ethanol, 70～80 ℃ of concentrating under reduced pressure, 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(4) mangosteen powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(5) get Herba Dendrobii extract fine powder 8g, wolfberry fruit extract fine powder 6g, Radix Panacis Quinquefolii extract fine powder 5g and Fructus Momordicae extract fine powder 4g mix homogeneously.
With obtained extract fine powder and dextrine powder, the microcrystalline Cellulose mix homogeneously of crossing 80 mesh sieves, spraying adds the alcohol granulation that is equivalent to described mixed material weight 5%, and drying adds the magnesium stearate mixing, tabletting, and packing namely gets the compound dendrobium officinale tablet.
Present embodiment, the adult takes twice, each 4 every day.
Embodiment 4
Present embodiment Herba Dendrobii compound capsule, its crude drug proportioning is:
Principal agent: Herba Dendrobii 50g, Fructus Lycii 40g, Radix Panacis Quinquefolii 25g, Fructus Momordicae 30g; Adjuvant: dextrin 10g, microcrystalline Cellulose 10g, magnesium stearate: 0.5g.
Preparation method:
(1) Herba Dendrobii is cut into 5 ~ 8 mm left and right sides thin segments, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(2) Fructus Lycii powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(3) Radix Panacis Quinquefolii powder is broken into coarse powder, and add 6 times of amount 60% alcohol heating reflux and extract three times, each 3 hours, merge extractive liquid,, Recycled ethanol, 70～80 ℃ of concentrating under reduced pressure, 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(4) mangosteen powder is broken into coarse powder, adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, 70～80 ℃ of concentrating under reduced pressure of filtrate, and 70～80 ℃ of dryings are pulverized, and are for subsequent use.
(5) get Herba Dendrobii extract fine powder 10g, wolfberry fruit extract fine powder 8g, Radix Panacis Quinquefolii extract fine powder 5g and Fructus Momordicae extract fine powder 6g mix homogeneously.
With obtained extract fine powder and dextrine powder, the microcrystalline Cellulose mix homogeneously of crossing 80 mesh sieves, spraying adds the alcohol granulation that is equivalent to described mixed material weight 5%, drying, granulate adds the magnesium stearate mixing, the fill capsule, packing namely gets the compound dendrobium officinale capsule.
Present embodiment, the adult takes twice, each 4 every day.
Herba Dendrobii compound preparation provided by the invention is carried out the test of pesticide effectiveness, confirm that through zoopery this product has the function of enhancing immunity and alleviating physical fatigue, particular content is as follows.
The enhancing immunity function test: per os gives this product 14 d of mice various dose, the result shows, this product can strengthen the carbon of macrophage and clean up ability, increase Spleen coefficient and B Proliferation of lymphocytes ability, show that said preparation has certain immunological enhancement, but the specificity of enhancing body and non-specific immunity.Therefore think that this product has the function of enhancing immunity.
1. test material and method.
Sample: the agent of Herba Dendrobii compound granular, specification: the 2.0g/ bag, press embodiment 1 described method preparation.
Laboratory animal and grouping: select 80 of cleaning level female mices to be divided into immediately two by body weight and to organize greatly available from Zhejiang Academy of Medical Sciences medical experiment animal center, 40 every group, carry out respectively the test of mice carbon clearance and immune organ and lymphocyte transformation test.
40 mices of mice carbon clearance test are divided into 4 groups, i.e. matched group, the basic, normal, high dosage group of Herba Dendrobii compound granular agent.Every group all by capacity gavages such as 20ml/ kg body weight, matched group gives distilled water, the administration group is diluted the agent of Herba Dendrobii compound granular with dissolved in distilled water, the concentration of basic, normal, high dosage group is respectively 10mg/ ml, 30mg/ ml, 100 mg/ ml, be that dosage is respectively 200mg/ kg body weight, 600mg/ kg body weight and 2000mg/ kg body weight, every day 1 time, continuous 14 days (d).Behind successive administration 14 d, calculate the india ink that the tail vein injection saline dilution is 5 times by 0. 1 ml/ 10g body weight.2 min, 10 min get blood 20 μ l from the eye socket venous plexus after injection, join 2 ml, 0. 1% Na
2CO
3In the solution, 600 nm wavelength are measured absorbances (OD) value, and mice is put to death, and get liver, spleen is weighed.Calculate phagocytic index (K) and proofread and correct phagocytic index (a).
Phagocytic index K=(logA1-logA2) ÷ (t1-t2)
Proofread and correct phagocytic index a=body weight/(liver weight+spleen is heavy) * K^3
Immune organ weight and organ coefficient: a same test is processed in the grouping of laboratory animal and administration.Behind administration 14 d, the abdominal vein sacrificed by exsanguination is taken out spleen, the thymus of animal and is weighed, and calculates organ index.
Organ index (%)=organ weights (mg)/the weight of animals (g) * 100
The mouse spleen lymphocyte conversion test: take out animal spleen, subtract brokenly, preparation cell suspension is adjusted concentration for being 106/ml after the cell technology, add 96 well culture plates.Every hole adds 90 μ l splenocyte suspensions, the ConA(concanavalin A of the 50 μ g/ ml of 10 μ l) or the LPS liquid (lipopo-lysaccharide, lipopolysaccharide claim again endotoxin) of 100 μ g/ ml.Matched group replaces ConA with 1640 culture fluid.Insert the CO of 5 %
237 ℃ of conditions are cultivated 68 h, every hole adds MTT liquid (3-(4,5)-dimethylthiahiazo (z-y1)-3,5-di-phenytetrazoliumromide, 3-(4,5-dimethylthiazole-2)-2,5-diphenyl tetrazole bromine salt, commodity are called tetrazolium bromide) 10 μ l, after continuing to cultivate 4 h, every hole adds 100 μ l, three liquid and leaves standstill 12h.Wherein three liquid are the SDS(sodium lauryl sulphate) 10g, isobutanol 5ml, 10M HCl 0.1ml are made into the solution of 100ml with the distilled water dissolving.Dissolve the rear enzyme-linked immunosorbent assay instrument of using fully until purple crystal and measure the OD value with 570 nm (master)/630nm (inferior) wavelength, deduct the OD value that does not add the ConA hole with the OD value that adds the ConA hole and represent T Proliferation of lymphocytes ability, deduct the multiplication capacity that the OD value that does not add the LPS hole represents bone-marrow-derived lymphocyte with the OD value that adds the LPS hole.
2. result.
Compare with matched group, the Herba Dendrobii compound granular agent group of 600 and 2000 mg/kg phagocytic index and proofread and correct phagocytic index and have significance to increase (P＜0.05), show that the agent of Herba Dendrobii compound granular can strengthen the phagocytic function of macrophage, accelerate the removing of carbon granules, referring to table 1.
The agent of table 1 Herba Dendrobii compound granular is on the impact of mouse immune organ weight and organ coefficient.Every group of number of samples n=10, data are that (mean ± s), * represents P＜0.05 to sample average ± sample error in the table.
| Group | Body weight (g) | Thymic weight (g) | The thymus coefficient | Spleen weight (g) | Spleen coefficient |
| Matched group | 26.6 ± 0.5 | 0.054 ± 0.023 | 0.24 ± 0.05 | 0.107 ± 0.06 | 0.41 ± 0.01 |
| Herba Dendrobii compound granular agent low dose group (200 mg/kg) | 26.4 ± 0.4 | 0.052 ± 0.018 | 0.22 ± 0.04 | 0.118 ± 0.03 | 0.45 ± 0.01* |
| Dosage group in the agent of Herba Dendrobii compound granular (600 mg/kg) | 27.6 ± 0.3 | 0.050 ± 0.016 | 0.21 ± 0.04 | 0.120 ± 0.06 | 0.46 ± 0.01* |
| Herba Dendrobii compound granular agent high dose group (2000 mg/kg) | 25.7 ± 0.4 | 0.056 ± 0.018 | 0.26 ± 0.06 | 0.122 ± 0.08 | 0.46 ± 0.01* |
The Herba Dendrobii compound granular agent of the continuous 14 days oral 200-2000 of giving mg/kg to laboratory animal body weight and thymus and spleen weight and Spleen coefficient do not make significant difference (with matched group than P 0. 05), but can cause the remarkable increase (P＜0. 05) of Spleen coefficient, referring to table 2.
Ability is cleaned up in the agent of table 2 Herba Dendrobii compound granular on mice carbon impact.Every group of number of samples n=10, data are that (mean ± s), * represents P＜0.05 to sample average ± sample error in the table.
| Group | Phagocytic index | Proofread and correct phagocytic index |
| Matched group | 0.0072 ± 0.005 | 3.57 ± 0.27 |
| Herba Dendrobii compound granular agent low dose group (200 mg/kg) | 0.0088 ± 0.012 | 3.72 ± 0.38 |
| Dosage group in the agent of Herba Dendrobii compound granular (600 mg/kg) | 0.0123± 0.03* | 4.55 ± 0.39* |
| Herba Dendrobii compound granular agent high dose group (2000 mg/kg) | 0.0134 ± 0.04* | 4.68 ± 0.50* |
In the T lymphocyte proliferation assay with the ConA stimulation, comparison there was no significant difference between each group (P〉0.05); In the B lymphocyte proliferation assay with the LPS stimulation, 600 relatively exist significant difference (P＜0.05) with difference and the matched group of 2000mg/kg dosage group, the DTP(dendrobium polysaccharide is described) have the lymphopoietic effect of the B of stimulation, referring to table 3.
The agent of table 3 Herba Dendrobii compound granular is on the impact of mouse T lymphocyte and bone-marrow-derived lymphocyte propagation.Every group of number of samples n=10, data are that (mean ± s), * represents P＜0.05 to sample average ± sample error in the table.
| Group | The OD(contrast) | OD（ConA） | OD difference (ConA) | OD（LPS） | OD difference (LPS) |
| Matched group | 0.101 ± 0.019 | 0.110 ± 0.017 | 0.011 ± 0.006 | 0.111± 0.014 | 0.011 ± 0.005 |
| Herba Dendrobii compound granular agent low dose group (200 mg/kg) | 0.113 ± 0.016 | 0.122± 0.021 | 0.010 ± 0.005 | 0.129 ± 0.021 | 0.015 ± 0.007 |
| Dosage group in the agent of Herba Dendrobii compound granular (600 mg/kg) | 0.107 ± 0.023 | 0.126 ± 0.022 | 0.019 ± 0.006 | 0.130 ± 0.018 | 0.023 ± 0.009* |
| Herba Dendrobii compound granular agent high dose group (2000 mg/kg) | 0.114 ± 0.018 | 0.129 ± 0.018 | 0.012 ± 0.009 | 0.133 ± 0.016 | 0.020± 0.010* |
Herba Dendrobii compound preparation provided by the invention, by Herba Dendrobii, Fructus Lycii, Radix Panacis Quinquefolii and Fructus Momordicae synergism, the crude polysaccharides that wherein contains, American ginseng total saponins etc. have immunomodulating, anti-fatigue effect, be applicable to the immunocompromised person, the fatiguability person, middle-aged and elderly people and be in all kinds of crowds of sub-health state also can be used for the rehabilitation behind the cancer patient chemicotherapy.
Although content of the present invention has been done detailed introduction by above preferred embodiment, will be appreciated that above-mentioned description should not be considered to limitation of the present invention.After those skilled in the art have read foregoing, for multiple modification of the present invention with to substitute all will be apparent.Therefore, protection scope of the present invention should be limited to the appended claims.
Claims (7)
1. a Herba Dendrobii compound preparation is characterized in that, this Herba Dendrobii compound preparation comprises following raw material:
Herba Dendrobii 2 ~ 60g, Fructus Lycii 2 ~ 60g, Radix Panacis Quinquefolii 1 ~ 50g, Fructus Momordicae 1 ~ 50g;
Described Herba Dendrobii, Fructus Lycii and Fructus Momordicae all carry out water extraction, and described Radix Panacis Quinquefolii carries out 40% ~ 60% ethanol extraction.
2. Herba Dendrobii compound preparation as claimed in claim 1 is characterized in that, also further includes adjuvant in the described Herba Dendrobii compound preparation; Described adjuvant comprises any one or a few the combination in 2 ~ 100g glucose, 2 ~ 100g lactose, 2 ~ 100g sucrose, 2 ~ 100g starch, 2 ~ 100g dextrin, 2 ~ 100g microcrystalline Cellulose, 0.2 ~ 10g magnesium stearate, 2 ~ 100g Mel and the 0.2 ~ 10g Pulvis Talci.
3. Herba Dendrobii compound preparation as claimed in claim 2 is characterized in that, the dosage form of described Herba Dendrobii compound preparation comprises tablet, capsule, granule, pill, powder, oral liquid, medicated wine or unguentum.
4. the preparation method such as the described Herba Dendrobii compound preparation of claim 1 ~ 3 is characterized in that, described preparation method comprises:
Step 1, Herba Dendrobii are cut into the segment that length is 5 ~ 8 mm, add the twelvefold water gaging and decoct each 3 hours three times, collecting decoction filters, and filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of crushed after being dried, obtain described Herba Dendrobii extract powder, for subsequent use;
Step 2, Fructus Lycii is pulverized, and adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, and filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of dryings, pulverizing obtains described Fructus Lycii extraction powder again, and is for subsequent use;
Step 3, Radix Panacis Quinquefolii is pulverized, and adds 6 times of amount 50% alcohol heating reflux and extracts each 3 hours three times, merge extractive liquid,, Recycled ethanol, filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of dryings, again pulverize, obtain described Radix Panacis Quinquefolii extract powder, for subsequent use;
Step 4, Fructus Momordicae is pulverized, and adds the twelvefold water gaging and decocts three times, and each 3 hours, collecting decoction filtered, and filtrate is in 70～80 ℃ of concentrating under reduced pressure, and in 70～80 ℃ of dryings, pulverizing obtains described Fructus Momordicae extract powder again, and is for subsequent use;
Step 5, with the Herba Dendrobii extract powder of step 1 to step 4 gained, Fructus Lycii extraction powder, Radix Panacis Quinquefolii extract powder and Fructus Momordicae extract powder mix homogeneously are processed into preparation.
5. the preparation method of Herba Dendrobii compound preparation as claimed in claim 4, it is characterized in that the Herba Dendrobii extract powder described in the step 5 is 0.4 ~ 12g, Fructus Lycii extraction powder is 0.4 ~ 12g, the Radix Panacis Quinquefolii extract powder is 0.2 ~ 10g, and the Fructus Momordicae extract powder is 0.2 ~ 10g.
6. the preparation method of Herba Dendrobii compound preparation as claimed in claim 4 is characterized in that, comprises further also in the described step 5 that adding the adjuvant post-treatment becomes preparation.
7. one kind such as the purposes of the described Herba Dendrobii compound preparation of claim 1 ~ 3 for the preparation of health care medicine.
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