CN108524921A - A kind of antiradiation injury protective agent and its preparation method and application - Google Patents
A kind of antiradiation injury protective agent and its preparation method and application Download PDFInfo
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Abstract
The present invention proposes a kind of antiradiation injury protective agent and its preparation method and application; a kind of antiradiation injury protective agent; including isoflavones nanometer formulation, collagen iron (II) chelate, Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet, dendrobium candidum; preparation method includes the following steps：(1) preparation of isoflavones nanometer formulation；(2) preparation of collagen iron (II) chelate；(3) the protectant preparation of antiradiation injury.Antiradiation injury protective agent of the present invention has the effect of there is apparent radiation protection in plant, animal and human body, has broad application prospects.
Description
Technical field
The present invention relates to pharmaceutical technology fields, and in particular to a kind of antiradiation injury protective agent and preparation method thereof and answers
With.
Background technology
China is Uranium industry big country, and the personnel for being engaged in uranium operation are up to a million, if adding relevant industry, practitioner is more
It is more.Show the healthy follow-up investigation of uranium operating personnel the incidence of a variety of diseases such as Long Term Contact Uranium-Mineral Dust and silicosis, lung cancer
Increase related.Guangxi District uranium ore resource is abundant, and the occupational hazards of uranium ore operation is also very common.Therefore, effectively prevent uranium ore
The medical protection measure of dirt damage has broad application prospects.
It is current research shows that, isoflavones have the protective action to blood vessel, similar female estrogen effect and
The different physiological roles such as antihormones effect, bacteriostatic activity and pre- anti-osteoporosis also have research to confirm that it may conduct recently
A kind of natural, and many natural antioxidants all have antitumor efficacy.Isoflavones radiation resistance table
It is now that can remove interior free yl to protect Cell membrane lipids and inhibit the free radical of radiation-induced generation to the damage of large biological molecule
Wound.Therefore the anti-oxidant and antitumor activity of isoflavones is studied, it is intended to be further developed as efficient, nontoxic
The novel antioxidant and antitumor drug of side effect provide theoretical foundation.By nanotechnology, it can effectively make preparation quilt
Cell absorbs.
Ferrous ion has stronger reproducibility, can be used as preferable antioxidant in vivo, restores radiation-induced fat
Matter peroxide or free radical, but due to the activity of ferrous ion, ferrous ion is hard to reach action site and is lost by oxidation
Effect.Ferrous ion chelating is got up, then can be very good protection ferrous ion, preferable chelating agent is then collagen, collagen
There are many potential biologically active peptide fragments in albumen, the collagen peptide of low molecular weight is easy to be absorbed by the body, and
With the ability significantly combined with divalent metal mineral ion (such as calcium ion, ferrous ion, copper ion), in molecular structure
The side groups such as amino, carboxyl, hydroxyl, arc base complexation reaction can occur with metal ion at different conditions, formed stable
The chelate of chelate, formation has the function of metallic ore substance and collagen peptide simultaneously.Studies have shown that collagen-iron
(II) chelate can promote by the injury repair according to rat.
Dai medicine Caulis Spatholobi be legume spatholobus suberectus drying rattan, have replenishing and activating blood, menstruction regulating and pain relieving, relaxing tendons and activating collaterals
Effect is widely used in China and country in Southeast Asia, is broadly divided into osajin, flavanone, flavane (alcohol) class, dihydro
Flavonols, procyanidine class, chalcones, pterocarpan, isoflavones alcohols, isoflavan class and aurones class have and adjust
Hematological system, radioresistance, inhibits a variety of pharmacological activity such as melanin pigmentation at antitumor, anti-oxidant, antiviral, antibacterial.
Tibetan medicine pine life etc. is Rhamnaceae plant Rhamnella gilgitica or the desiccated wood of Fructus Rhamni parvifoliae, is Tibetan medicine and pharmacology conventional crude drugs,
Its standard is recorded issues in 1995 editions portions《Drug standards Tibetan medicine》First.Traditional Tibetan medicine and pharmacology theory thinks that pine life etc. has cool blood
The effect of detumescence, is clinically used for rheumatoid arthritis, grasserie, the more blood diseases of high mountain.Modern study finds that the raw extract of pine has
Apparent anti-oxidant, radiation protection, there are apparent dose-effect relationships with sample concentration for clearance rate.
Invention content
In order to solve the above technical problems, a kind of antiradiation injury protective agent of present invention offer and preparation method thereof, and
The application that a kind of antiradiation injury protective agent is used for radiation protection is provided.The antiradiation injury protective agent is by isoflavones nanometer system
Agent, collagen-iron (II) chelate and one group of Chinese medicine composition are prepared, and are experimentally confirmed antiradiation injury protection
Agent has apparent radiation resistance to human body, animals and plants.
The present invention provides a kind of antiradiation injury protective agent, is prepared by following raw material：Isoflavones is received
15-20 parts of metric system agent, 15-20 parts of collagen-iron (II) chelate, Caulis Spatholobi 10-15 parts, 10-15 parts of flower of kudzuvine, the raw 10- of pine
15 parts, 5-10 parts of grape pip, 5-10 parts of glossy privet, 2-7 parts of dendrobium candidum.
As further improvement of the invention, protective agent is prepared by following raw material：Isoflavones nanometer
18 parts of preparation, 17 parts of collagen-iron (II) chelate, 13 parts of Caulis Spatholobi, 12 parts of flower of kudzuvine, 12 parts of pine life, 7 parts of grape pip, female
Loyal 7 parts, 5 parts of dendrobium candidum.
The present invention further protects the protectant preparation method of above-mentioned antiradiation injury, includes the following steps：
(1) preparation of isoflavones nanometer formulation：Isoflavones is dissolved in ethyl acetate and is used as oil phase, will be oily
It is mutually scattered in water phase, using quick emulsification equipment, under the pressure of CO2, cycle obtains oil-in-water emulsion, is evaporated off under reduced pressure second
Acetoacetic ester obtains isoflavones aqueous nanoparticle dispersion liquid, is freeze-dried to get isoflavones nanometer formulation；
(2) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron is prepared
(II) chelate；
(3) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, adds
Enter 8 times of volume of water, impregnate 30min, decocts 2h, be filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step,
1h is decocted, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, isoflavones nanometer formulation and collagen-iron (II) chelate is added, adds auxiliary material, various dosage forms are made.
As further improvement of the invention, it is dissolved in 5mL ethyl acetate per 1g isoflavones.
As further improvement of the invention, the preparation condition of collagen-iron (II) chelate is that microwave power is
500W, microwave reaction time are 15min, and the molar ratio of collagen ligand and ferrous ion is 1:1.
As further improvement of the invention, the raw material of ferro element can be in the preparation of collagen-iron (II) chelate
Ferrous sulfate, frerrous chloride, ferrous nitrate.
As further improvement of the invention, the raw material of collagen can in the preparation of collagen-iron (II) chelate
Collagen peptide liquid is digested for human-like collagen, Lip river phenanthrene fish scale collagen, tilapia fishskin collagen polypeptide, channel catfish.
Improved as of the invention further, dosage form include externally used paste, liniment and it is interior with granule, tablet, oral solution,
Capsule, pill.
The present invention further protects application of the above-mentioned antiradiation injury protective agent for plant radiation protection.
The present invention further protects application of the above-mentioned antiradiation injury protective agent for human body and animal radiation protection.
The present invention has the advantages that：
1, the present invention prepares isoflavones nanometer formulation, is easy to be absorbed, effect is good, and radioresistance is with obvious effects；
2, the present invention combines isoflavones nanometer formulation, metallo-chelate and Chinese herbal medicine, prepares novel anti-spoke
Injury protection agent is penetrated, and being experimentally confirmed the antiradiation injury protective agent has apparent radioresistance to make human body, animals and plants
With；
3, the antiradiation injury protective agent is prepared simple, and using wide, the dosage form that can be prepared is more, and application mode is various, can
Meets the needs of different crowd.
Description of the drawings
Fig. 1 is the preparation technology flow chart of isoflavones nanometer formulation.
Specific implementation mode
Below in conjunction with the embodiment of the present invention, technical solution in the embodiment of the present invention carries out clear, complete description,
Obviously, the embodiment described is the embodiment of the part of representative of the present invention, rather than whole embodiments, this field are general
Other all embodiments that logical technical staff is obtained without making creative work belong to the protection of the present invention
Range.
The preparation of 1 antiradiation injury screening glass of embodiment
Raw material forms：20 parts of isoflavones nanometer formulation, 20 parts of collagen-iron (II) chelate, 15 parts of Caulis Spatholobi,
15 parts of flower of kudzuvine, 15 parts of pine life, 10 parts of grape pip, 10 parts of glossy privet, 7 parts of dendrobium candidum；
Preparation process：
(1) preparation of isoflavones nanometer formulation：50g isoflavones is dissolved in 150mL ethyl acetate as oil
Phase is scattered in by oil phase in water phase, using quick emulsification equipment, in CO2Pressure under, cycle obtain oil-in-water emulsion, subtract
Pressure evaporates ethyl acetate, obtains isoflavones aqueous nanoparticle dispersion liquid, is freeze-dried to get isoflavones nanometer system
Agent；
(2) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron is prepared
(II) chelate, microwave power 400W, microwave reaction time are 10min, the molar ratio of human-like collagen and ferrous sulfate
It is 1:2；
(3) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, adds
Enter 8 times of volume of water, impregnate 30min, decocts 2h, be filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step,
1h is decocted, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, isoflavones nanometer formulation and collagen-iron (II) chelate is added, adds maltodextrin and is spray-dried to obtain
Spray powder, yield 62%.Magnesium stearate, silica and microcrystalline cellulose, mixing are added, antiradiation injury guarantor is made in tabletting
Bluff piece.
2 antiradiation injury of embodiment protects the preparation of particle
Raw material forms：18 parts of isoflavones nanometer formulation, 17 parts of collagen-iron (II) chelate, 13 parts of Caulis Spatholobi,
12 parts of flower of kudzuvine, 12 parts of pine life, 7 parts of grape pip, 7 parts of glossy privet, 5 parts of dendrobium candidum.
Preparation process：
(1) preparation of isoflavones nanometer formulation：50g isoflavones is dissolved in 200mL ethyl acetate as oil
Phase is scattered in by oil phase in water phase, using quick emulsification equipment, in CO2Pressure under, cycle obtain oil-in-water emulsion, subtract
Pressure evaporates ethyl acetate, obtains isoflavones aqueous nanoparticle dispersion liquid, is freeze-dried to get isoflavones nanometer system
Agent；
(2) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron is prepared
(II) chelate, microwave power 400W, microwave reaction time are 10min, the molar ratio of human-like collagen and ferrous sulfate
It is 1:2；
(3) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, adds
Enter 8 times of volume of water, impregnate 30min, decocts 2h, be filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step,
1h is decocted, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, isoflavones nanometer formulation and collagen-iron (II) chelate is added, adds maltodextrin and is spray-dried to obtain
Spray powder, yield 78%.The ethanol solution of addition 85% makes pellet, dry, whole grain, packaging, and antiradiation injury protection is made
Grain.
The protectant preparation of 3 antiradiation injury of embodiment
Raw material forms：18 parts of isoflavones nanometer formulation, 17 parts of collagen-iron (II) chelate, 13 parts of Caulis Spatholobi,
12 parts of flower of kudzuvine, 12 parts of pine life, 7 parts of grape pip, 7 parts of glossy privet, 5 parts of dendrobium candidum.
Preparation process：
(1) preparation of isoflavones nanometer formulation：50g isoflavones is dissolved in 250mL ethyl acetate as oil
Phase is scattered in by oil phase in water phase, using quick emulsification equipment, in CO2Pressure under, cycle obtain oil-in-water emulsion, subtract
Pressure evaporates ethyl acetate, obtains isoflavones aqueous nanoparticle dispersion liquid, is freeze-dried to get isoflavones nanometer system
Agent；
(2) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron is prepared
(II) chelate, microwave power 500W, microwave reaction time are 15min, and Lip river phenanthrene fish scale collagen and frerrous chloride rub
You are than being 1:1；
(3) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, adds
Enter 8 times of volume of water, impregnate 30min, decocts 2h, be filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step,
1h is decocted, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, isoflavones nanometer formulation and collagen-iron (II) chelate is added, adds maltodextrin and is spray-dried to obtain
Spray powder, yield 90%.The ethanol solution of addition 85% makes pellet, dry, whole grain, packaging, and antiradiation injury protection is made
Grain.
4 antiradiation injury of embodiment protects the preparation of capsule
Raw material forms：15 parts of isoflavones nanometer formulation, 15 parts of collagen-iron (II) chelate, 10 parts of Caulis Spatholobi,
10 parts of flower of kudzuvine, 10 parts of pine life, 5 parts of grape pip, 5 parts of glossy privet, 2 parts of dendrobium candidum.
Preparation process：
(1) preparation of isoflavones nanometer formulation：50g isoflavones is dissolved in 300mL ethyl acetate as oil
Phase is scattered in by oil phase in water phase, using quick emulsification equipment, in CO2Pressure under, cycle obtain oil-in-water emulsion, subtract
Pressure evaporates ethyl acetate, obtains isoflavones aqueous nanoparticle dispersion liquid, is freeze-dried to get isoflavones nanometer system
Agent；
(2) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron is prepared
(II) chelate, microwave power 600W, microwave reaction time are 30min, the molar ratio of human-like collagen and ferrous sulfate
It is 2:1；
(3) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, adds
Enter 8 times of volume of water, impregnate 30min, decocts 2h, be filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step,
1h is decocted, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, isoflavones nanometer formulation and collagen-iron (II) chelate is added, adds maltodextrin, is spray-dried, obtains
To spray powder, yield 81%.Prepare No. 2 capsulae vacuus, is sealed after spray powder is filled to capsule, obtain antiradiation injury Protection glue
Capsule.
Antiradiation injury of the embodiment 5 without isoflavones nanometer formulation protects particle
Raw material forms：17 parts of collagen-iron (II) chelate, 13 parts of Caulis Spatholobi, 12 parts of flower of kudzuvine, pine raw 12 parts, grape
7 parts of seed, 7 parts of glossy privet, 5 parts of dendrobium candidum.
Preparation process：
(1) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron is prepared
(II) chelate, microwave power 500W, microwave reaction time are 15min, and Lip river phenanthrene fish scale collagen and frerrous chloride rub
You are than being 1:1；
(2) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, adds
Enter 8 times of volume of water, impregnate 30min, decocts 2h, be filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step,
1h is decocted, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, collagen-iron (II) chelate is added, adds maltodextrin and is spray-dried to obtain spray powder, yield 90%.Addition
85% ethanol solution makes pellet, dry, whole grain, packaging, and antiradiation injury protection particle is made.
1 mouse test of test example
(1) animal and grouping：Cleaning grade SD male rats 200,8~9 week old (200 ± 20g of weight), are purchased from military doctor
Subject institute Experimental Animal Center.Irradiation control group, Anduo County's continuous heavy rain Capsules group (1 group), embodiment 3 are randomly divided by weight to prepare
Antiradiation injury protects the antiradiation injury protection without isoflavones nanometer formulation prepared by particle group (2 groups), embodiment 5
Particle group (3 groups) and blank group, every group of 40 rats.
(2) experimental method：Anduo County's continuous heavy rain Capsules group (1 group)：It is primary that 12g/kg/d, predose 7d start daily gavage, and totally 7
It is secondary；Antiradiation injury protection particle group (2 groups) (12g/kg/d) and embodiment 5 prepared by embodiment 3 is prepared different without soybean
The antiradiation injury protection particle group (3 groups) (12g/kg/d) of flavones nanometer formulation, it is primary that predose 7d starts daily gavage, altogether
7 times；Control group and blank group give isometric distilled water.
Radiation parameter：Control group and 1-3 group rats use dedicated rat irradiation devices, using microwave source single whole body one
Secondary irradiation 15min, average power density 30mW/cm2.In the detection for carrying out various pharmacodynamic indexs according to rear 7d and 14d.
Experiment one：Antiradiation injury protects influence of the particle to microwave irradiation rat nerve behaviouristics obstacle
Morris water maze tests, for generally accepted measurement animal (mainly large and small mouse) learning and memory at present
The experiment of ability.Rat finds the ability of platform after the experiment is mainly handled with various factors, i.e., so-called escape latency is
Basis, the ability of learning and memory of overall merit rat.This experiment detects learning and memory in rats energy using Morris water maze tests
The change of power, using escape latency (day) as observation index.
Experimental result is referring to table 1.
1 antiradiation injury of table protects particle to 30mW/cm2The shadow of microwave irradiation treated rats in Morris water maze performance escape latency
It rings
As seen from Table 1, four groups of rats show that each group rat exists as the increase of frequency of training, escape latency are shortened
Platform capabilities are found in learning training gradually to be enhanced.With irradiation control group ratio, successive administration 7d, the 1d after drug withdrawal, embodiment 3 is made
The antiradiation injury protection without isoflavones nanometer formulation of standby antiradiation injury protection particle group, the preparation of embodiment 5
Particle group and Anduo County's continuous heavy rain Capsules group escape latency have the tendency that shortening, and antiradiation injury prepared by the present invention protects particle
Effect is optimal.And 2-6d after being discontinued, the time relatively irradiation control group of three experimental group escape latencies are obviously shortened, and show to pacify
More continuous heavy rains and the present invention prepare antiradiation injury protection particle, the antiradiation injury protection particle without isoflavones nanometer formulation
Play the role of improving the learning and memory in rats obstacle that microwave induces, the 6th day after being especially discontinued, the anti-spoke of the preparation of embodiment 3
Penetrate the antiradiation injury without isoflavones nanometer formulation that injury protection particle group improvement result is superior to the preparation of embodiment 5
Particle group and Anduo County's continuous heavy rain group are protected, normal level is tended to.
The influence for the hematopoietic disorder that experiment two, antiradiation injury protection particle induce microwave
7d and 14d takes the detection of blood progress hematopoiesis function index from experimental rat tail vein after microwave irradiation.
Experimental result is shown in Table 2.
2 antiradiation injury of table protects particle to 30mW/cm2Microwave irradiation rat peripheral blood WBC and lymphocyte absolute number
Influence
Three groups of mouse peripheral blood total white blood cells (WBC) and lymphocyte absolute number occur increasing (table compared with control group
2).Each group mouse week blood leukocytes total (WBC) and lymphocyte number 14d have a recovery compared to 7d, and prepared by the present invention
Antiradiation injury protects particle to restore best, and number tends to normal level after 14d.Prepared by embodiment 5 is free of the different Huang of soybean
The antiradiation injury protection particle of ketone nanometer formulation is more excellent compared to positive drug control Anduo County continuous heavy rain capsule effect.
Comprehensive analysis the above results, it can be seen that the life that antiradiation injury protection particle prepared by the present invention induces microwave
The damage of object has comprehensive improvement result, can improve the neuroethology obstacle of microwave induction and promote hematopoiesis, is immunized
The recovery of function, and better than positive control drug Anduo County continuous heavy rain of unique resisting microwave radiation damage domestic at present in many indexs
Capsule.
Those skilled in the art is not under conditions of departing from the spirit and scope of the present invention of claims determination, also
Various modifications can be carried out to the above content.Therefore the scope of the present invention is not limited in above explanation, but by
The range of claims determines.
Claims (10)
1. a kind of antiradiation injury protective agent, which is characterized in that the protective agent is prepared by following raw material：Soybean
15-20 parts of isoflavones nanometer formulation, 15-20 parts of collagen-iron (II) chelate, Caulis Spatholobi 10-15 parts, 10-15 parts of flower of kudzuvine,
10-15 parts of pine life, 5-10 parts of grape pip, 5-10 parts of glossy privet, 2-7 parts of dendrobium candidum.
2. a kind of antiradiation injury protective agent according to claim 1, which is characterized in that the protective agent is by following parts by weight
Raw material is prepared：18 parts of isoflavones nanometer formulation, 17 parts of collagen-iron (II) chelate, 13 parts of Caulis Spatholobi, flower of kudzuvine
12 parts, pine is 12 parts raw, 7 parts of grape pip, 7 parts of glossy privet, 5 parts of dendrobium candidum.
3. a kind of protectant preparation method of antiradiation injury according to claim 1, which is characterized in that the method includes
Following steps：
(1) preparation of isoflavones nanometer formulation：Isoflavones is dissolved in ethyl acetate and is used as oil phase, is divided by oil phase
It dissipates in water phase, using quick emulsification equipment, in CO2Pressure under, cycle obtain oil-in-water emulsion, be evaporated off under reduced pressure acetic acid second
Ester obtains isoflavones aqueous nanoparticle dispersion liquid, is freeze-dried to get isoflavones nanometer formulation；
(2) preparation of collagen-iron (II) chelate：Using solid-state reaction under microwave technology, collagen-iron (II) chela is prepared
Close object；
(3) the protectant preparation of antiradiation injury：It takes Caulis Spatholobi, flower of kudzuvine, Song Sheng, grape pip, glossy privet to be mixed after crushing, is added 8
Times volume of water impregnates 30min, decocts 2h, is filtered under diminished pressure, filtrate is spare；5 times of volume of water will be added in the dregs of a decoction obtained by upper step, decocts
1h is boiled, is filtered under diminished pressure, filtrate is spare；It gained filtrate will merge twice, and cross 400 mesh sieve, concentration filtrate to relative density 0.9-
1.3, isoflavones nanometer formulation and collagen-iron (II) chelate is added, adds auxiliary material, various dosage forms are made.
4. preparation method according to claim 3, which is characterized in that described to be dissolved in 5mL ethyl acetate per 1g isoflavones
In.
5. preparation method according to claim 3, which is characterized in that collagen-iron (II) chelate prepares item
It is 500W that part, which is microwave power, and the microwave reaction time is 15min, and the molar ratio of collagen ligand and ferrous ion is 1:1.
6. preparation method according to claim 3, which is characterized in that in the preparation of collagen-iron (II) chelate
The raw material of ferro element can be ferrous sulfate, frerrous chloride, ferrous nitrate.
7. preparation method according to claim 3, which is characterized in that in the preparation of collagen-iron (II) chelate
The raw material of collagen can be human-like collagen, Lip river phenanthrene fish scale collagen, tilapia fishskin collagen polypeptide, channel catfish
Digest collagen peptide liquid.
8. preparation method according to claim 3, which is characterized in that the dosage form includes externally used paste, liniment and interior use
Granula, tablet, oral solution, capsule, pill.
9. a kind of antiradiation injury protective agent prepared according to claim 3 is used for the application of plant radiation protection.
10. a kind of antiradiation injury protective agent prepared according to claim 3 is used for the application of human body and animal radiation protection.
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| CN114306275A (en) * | 2021-12-24 | 2022-04-12 | 齐鲁工业大学 | Pea peptide-loaded soybean isoflavone composite nanoparticle and preparation method and application thereof |
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