CN103006903A - Compound preparation with anti-ageing effect - Google Patents
Compound preparation with anti-ageing effect Download PDFInfo
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- CN103006903A CN103006903A CN2012105933345A CN201210593334A CN103006903A CN 103006903 A CN103006903 A CN 103006903A CN 2012105933345 A CN2012105933345 A CN 2012105933345A CN 201210593334 A CN201210593334 A CN 201210593334A CN 103006903 A CN103006903 A CN 103006903A
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Abstract
The invention relates to an anti-ageing compound preparation and a preparation process and application thereof. The formula of the anti-ageing compound preparation particularly comprises the following materials in parts by weight: 5 parts of cornua cervi pantotrichum, 20 parts of medlar, 20 parts of polygonum multiflorum and 20 parts of semen sesami nigrum. The anti-ageing compound preparation disclosed by the invention is extracted by adopting a reflux method, wherein the cornua cervi pantotrichum and the medlar are extracted by taking water as a solvent, and the polygonum multiflorum and the semen sesami nigrum are extracted by taking alcohol as a solvent, an optimal extraction condition is selected according to an orthogonal experiment theory to finally prepare an oral solution. Animal experiments are carried out to prove that the anti-ageing compound preparation has anti-ageing and antioxidant capacity enhancing effects.
Description
Technical field
The present invention relates to a kind of compound preparation with anti-aging effects, be specifically related to preparation technology and the effect thereof of this compound preparation, belong to field of health care products.
Background technology
The current international problem of an aging population is on the rise, and under the heavy pressure of work and life, people be subject to the stimulations such as a large amount of radiation every day and diet also irregular and healthy, these all cause old and feeble becoming younger of occuring, and therefore inquire into the study hotspot that Aging Mechanism and defying age method have become medical domain.After aging referred to that body growth is reached maturity, the physiology merit of body declined and can descend gradually, and degenerative change progressively occurs histoorgan, and final deathward process, and degeneration wherein changes and must occur and be difficult to reverse in the life cycle of bion.
In that these are explained in the theory or theory of Aging mechanism at present, paid attention to most with free radical theory.Free radical theory is that HARMAND is theoretical at the oxygen-derived free radicals of the relevant aging of proposition in 1956, and this theory points out that not only free radical is to cause old and feeble key factor, and is that antioxidation and antidotal research point understand the most positive meaning.Oxidative stress refers to body when suffering various destructive stimulus, breaks the equilibrium relation between vivo oxidation and the antioxidant system, makes free-radical generating too much or the decline of removing ability, causes cell ageing dead.Therefore, oxidative stress is considered to cause a key factor of senescence and disease, and people just solve in its corresponding mode---and antioxidant reaches real antidotal purpose.In a word, antioxidant is by improving antioxidant ability of organism, remove senile organism and produce too much free radical, suppress the peroxidating process of body, tissue, cell and play a role, so select the best very worth further investigation of antioxidant performance senile-resistant efficacy.
Medicine has its unique method and theory aspect slow down aging, wherein Chinese medicine compound has irreplaceable advantage and huge potentiality, and has obtained a series of achievements.Dan Baozhi proposes in " matrimony vine polygonum multiflorum thumb medicated wine ", and Fructus Lycii and Radix Polygoni Multiflori compatibility can obviously strengthen its single drug effect.Existing medical book record, in the Marrow Nourishing Pill, Semen Sesami Nigrum can with the Cornu Cervi Pantotrichum compatibility; In the life-prolonging pellet, Radix Polygoni Multiflori and Semen Sesami Nigrum; In the U.S. temples pellet of Sapporo, Radix Polygoni Multiflori can all can reach with the Fructus Lycii compatibility effect of life lengthening.The antiaging agent of commonly using on the market now, such as vitamin E, the ginsenoside, melatonin etc., medicinal ingredient is single, and effect is desirable not to the utmost.And the present invention is combined to form compound preparation with Cornu Cervi Pantotrichum, Radix Polygoni Multiflori, Fructus Lycii and Semen Sesami Nigrum and appropriate vitamin, each component has respectively the effect of antioxidation, blood sugar lowering, blood fat reducing and increase immunologic function in this compound preparation, complementary compatibility synergism is obvious, replenish the limitation of single Chinese medicine, thereby reach the effect of antioxidation and slow down aging.
Summary of the invention
The purpose of this invention is to provide a kind of new health product with anti-aging effects that formed by Cornu Cervi Pantotrichum, Radix Polygoni Multiflori, Semen Sesami Nigrum and Fructus Lycii.
The property of medicine of single medicinal material of the present invention:
Cornu Cervi Pantotrichum: be the young horn of the unossified close living fine hair of stag of the animal Cervus nippon Temminck of Chordata Mammalia Cervidae or Cervus elaphus linnaeus.Sweet in the mouth, warm in nature, the main stagnant blood that leaks down, cold and heat infantile convulsion, the strong will of QI invigorating, the population are not old.
Radix Polygoni Multiflori: Radix Polygoni Multiflori is the traditional tonic medicine of commonly using, and is the dried root of polygonum multiflorum thunb.Warm in nature, bitter in the mouth, sweet, puckery.Function cures mainly: invigorating the liver and kidney, benefiting essence-blood, black beard and hair, bone and muscle strengthening, life lengthening.
Semen Sesami Nigrum: be the black seed of Pedaliaceae sesame, sweet in the mouth, property is flat, has invigorating the liver and kidney, benefiting essence-blood, the effect of foster the five internal organs and slow down aging.
Fructus Lycii: Fructus Lycii is the bent plant of Solanaceae Chinese holly rake of Qi mature fruit.Nature and flavor are sweet flat, return the Liver and kidney warp.Function cures mainly: invigorating the liver and kidney, beneficial vital essence, longue meat, improve complexion, make eye bright and calm the nerves, dispel the wind and control void, life lengthening, hard muscles and bones.Make Chinese medicine preparation (consumption is weight portion) according to following prescription:
5～10 parts in Cornu Cervi Pantotrichum, 20～25 parts of Radix Polygoni Multiflori, 20～25 parts of Semen Sesami Nigrums, 20～25 parts of Fructus Lyciis.
Optimum ratio of the present invention is:
5 parts in Cornu Cervi Pantotrichum, 20 parts of Radix Polygoni Multiflori, 20 parts of Semen Sesami Nigrums, 20 parts of Fructus Lyciis.
The present invention has the preparation technology of the functional food of anti-aging effects, may further comprise the steps:
1) take by weighing in proportion Radix Polygoni Multiflori, 60% alcohol reflux 2～3 hours that Semen Sesami Nigrum adds 6～8 times of weight extracts 2 times altogether, with sieve elimination medicinal residues, merges the alcohol extract that extracts for 2 times, and is in the water-bath Back stroke to distinguishing the flavor of without alcohol, for subsequent use;
2) take by weighing in proportion Cornu Cervi Pantotrichum, Fructus Lycii adds the water soaking 2～3 hours of 6～8 times of weight, carries out reflux, extract, 2～3 hours, extracts altogether 3 times, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions, and is for subsequent use;
3) medicinal liquid of combining step 1 and step 2 is concentrated into 1/4 of cumulative volume, sucking filtration, and filtrate adds dehydrated alcohol to 50% determining alcohol to remove impurity, and room temperature leaves standstill 48h;
4) medicinal liquid with step 3 carries out sucking filtration, and filtrate places the water-bath Back stroke to nothing alcohol flavor, sucking filtration, and filtrate adds dehydrated alcohol to 75% determining alcohol to remove impurity, cold preservation 48h;
5) medicinal liquid behind step 4 precipitate with ethanol is carried out sucking filtration, filtrate places the water-bath Back stroke to nothing alcohol flavor, sucking filtration, and filtrate adds 2.5 times water, boils 10min, is cooled to room temperature, cold preservation 48h;
6) medicinal liquid that extracts the step 5 contain the some grams of crude drug amount is diluted to 50mL, then adds 500mg taurine, 500mg folic acid, 500mg vitamin E, 5mg vitamin B
2, the 20mg vitamin B
3, the 5mg vitamin B
6, add 5mg sucrose, 0.5mL glycerol, 20mg potassium sorbate, 150mg polyvinylpyrrolidone, 1g polyoxyethylene hydrogenated Oleum Ricini, transfer pH to 3～4 to make this oral liquid with citric acid.
Usage and dosage: 1 times/day; 50mL/ time.
Prescription analysis: Cornu Cervi Pantotrichum, Radix Polygoni Multiflori, Fructus Lycii, Semen Sesami Nigrum is the common medicine of tonifying Qi of the kidney, existing medical book record, in the Marrow Nourishing Pill, Semen Sesami Nigrum and Cornu Cervi Pantotrichum compatibility can be used for treating old people's lumbago due to renal deficiency; In the life-prolonging pellet, Radix Polygoni Multiflori and Semen Sesami Nigrum have the effect of life lengthening; In the U.S. temples pellet of Sapporo, Radix Polygoni Multiflori can with the same usefulness of Fructus Lycii, the treatment blood and essence asthenia also has the effect of black hair in addition; Cornu Cervi Pantotrichum and Fructus Lycii are with nourishing the effect of prolonging life with also arriving.Polysaccharide component in Cornu Cervi Pantotrichum and the Fructus Lycii, stilbene glycoside in the Radix Polygoni Multiflori and the melanin in the Semen Sesami Nigrum, all has very strong oxidation and removing free radicals effect, alleviate the damage effect of free radical, reduce the generation of lipid peroxidation and the generation of lipid peroxidation product, thus effective slow down aging.This research utilizes four complementary compatibility synergism in conjunction with above four kinds, makes its drug effect performance to ultimate attainment, has also added simultaneously the effect that vitamin E and taurine and Chinese medicine ingredients are brought into play defying age jointly.The vitamin E Wheat Protein can strengthen the capillary of skin resistance, and keeps normal permeability, improve blood circulation and adjust reproductive function, anti-aging effects etc., simultaneously, the melanin of vitamin E in Semen Sesami Nigrum is combined, and its antioxidation is doubled.And taurine can promote to improve the pituitary hormone secretion organism endocrine system mode, and can promote immunity of organisms and antioxidation.
Good effect of the present invention is: compound preparation, and the science compatibility, toxic and side effects is little, and mouthfeel is all good, is easily absorbed by the body, and side effect is little, and indication is wide, and the remarkable enhancing body antioxidation of energy also delays body aging.
The specific embodiment
Embodiment 1
1) take by weighing Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 20g mixes, and adds 6 times of weight, 60% alcohol reflux 3 hours, extracts altogether 2 times, with sieve elimination medicinal residues, merges the alcohol extract of 2 extractions, and is in the water-bath Back stroke to without the alcohol flavor, for subsequent use;
2) take by weighing Cornu Cervi Pantotrichum 5g, Fructus Lycii 20g mixes, and adds 7 times of weight water soakings 3 hours, and reflux, extract, 2 hours is extracted 3 times altogether, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions, and is for subsequent use;
3) combining step 1) with step 2) medicinal liquid, be concentrated into 1/4 of cumulative volume, sucking filtration, filtrate adds dehydrated alcohol to 50% determining alcohol to remove impurity, room temperature leaves standstill 48h;
4) with step 3) medicinal liquid carry out sucking filtration, filtrate places the water-bath Back stroke to without alcohol flavor, sucking filtration, filtrate adds dehydrated alcohol to 75% determining alcohol to remove impurity, cold preservation 48h;
5) with step 4) medicinal liquid behind the precipitate with ethanol carries out sucking filtration, and filtrate places the water-bath Back stroke to without the alcohol flavor, sucking filtration, filtrate adds 2.5 times water, boils 10min, is cooled to room temperature, cold preservation 48h;
6) extract the step 5 contain crude drug amount 2.15 grams) medicinal liquid be diluted to 50mL, then add 500mg taurine, 500mg vitamin E, vitamin B
25mg, vitamin B
320mg, vitamin B
65mg, adding sucrose 5mg, glycerol 0.5mL, potassium sorbate 20mg, polyvinylpyrrolidone 150mg, polyoxyethylene hydrogenated Oleum Ricini 1g, citric acid transfer pH to 3.8 to make oral liquid.
Embodiment 2
1) take by weighing Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 25g mixes, and adds 7 times of weight, 60% alcohol reflux 3 hours, extracts altogether 2 times, with sieve elimination medicinal residues, merges the alcohol extract of 2 extractions, and is in the water-bath Back stroke to without the alcohol flavor, for subsequent use;
2) take by weighing Cornu Cervi Pantotrichum 10g, Fructus Lycii 20g mixes, and adds 6 times of weight water soakings 3 hours, and reflux, extract, 2 hours is extracted 3 times altogether, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions, and is for subsequent use;
3)～6) with embodiment 1 step spiral shell 3)～6).
Embodiment 3
1) take by weighing Radix Polygoni Multiflori 25g, Semen Sesami Nigrum 25g mixes, and adds 8 times of weight, 60% alcohol reflux 3 hours, extracts altogether 2 times, with sieve elimination medicinal residues, merges the alcohol extract of 2 extractions, and is in the water-bath Back stroke to without the alcohol flavor, for subsequent use;
2) take by weighing Cornu Cervi Pantotrichum 5g, Fructus Lycii 25g mixes, and adds 8 times of weight water soakings 3 hours, and reflux, extract, 2 hours is extracted 3 times altogether, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions, and is for subsequent use;
3)～6) with embodiment 1 step 3)～6).
Embodiment 4
1) take by weighing Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 25g mixes, and adds 6 times of weight, 60% alcohol reflux 3 hours, extracts altogether 2 times, with sieve elimination medicinal residues, merges the alcohol extract of 2 extractions, and is in the water-bath Back stroke to without the alcohol flavor, for subsequent use;
2) take by weighing Cornu Cervi Pantotrichum 10g, Fructus Lycii 25g mixes, and adds 7 times of weight water soakings 3 hours, and reflux, extract, 2 hours is extracted 3 times altogether, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions, and is for subsequent use;
3)～6) with embodiment 1 step 3)～6).
Embodiment 5
1) take by weighing Radix Polygoni Multiflori 20g, Semen Sesami Nigrum 20g mixes, and adds 7 times of weight, 60% alcohol reflux 3 hours, extracts altogether 2 times, with sieve elimination medicinal residues, merges the alcohol extract of 2 extractions, and is in the water-bath Back stroke to without the alcohol flavor, for subsequent use;
2) take by weighing Cornu Cervi Pantotrichum 10g, Fructus Lycii 25g mixes, and adds 7 times of weight water soakings 3 hours, and reflux, extract, 2 hours is extracted 3 times altogether, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions, and is for subsequent use;
3)～6) with embodiment 1 step 3)～6)
Following pharmacological evaluation proves therapeutic effect of the present invention:
By testing the beneficial effect of further elaboration functional food of the present invention, pass through survival assay, lipid peroxide catabolite malonaldehyde (MDA) assay in the serum, superoxide dismutase in the blood (SOD) vitality test is determined the defying age effect of functional food.
Experimental drug: positive drug is the preparation of ginsenoside, embodiment 1～5 preparation.
Laboratory animal: BALB/C mice body weight 18～22g, single sex is provided by Jilin University's Experimental Animal Center.
1. dosage grouping and animal are processed
60 mices are divided into six groups at random by body weight, be normal group, model group, positive controls (ginsenoside 200mg/kg), low dose group (investigational agent 100mg/kg), middle dosage group (investigational agent 200mg/kg), high dose group (investigational agent 400mg/kg).Group except normal group, all the other five groups of strength butt hemostasis D-galactose every day (120mg/kg) solution once, normal group injection every day waits the dosage normal saline.Simultaneously, the administration group is used the investigational agent gavage of various dose every day, positive controls ginsenoside's gavage, and normal group and model group are used the normal saline gavage of equivalent every day, and gavage is 30 days continuously.
2. detection index
1) lipid peroxide catabolite malonaldehyde (MDA) assay in the serum
Second day after the last administration is plucked the eyeball blood sampling, centrifuging and taking serum, and malonaldehyde (MDA) test kit is purchased from Nanjing and builds up bio-engineering research institute, measures with microplate reader.
2) superoxide dismutase (SOD) vitality test in the blood
Second day after the last administration is plucked the eyeball blood sampling, centrifuging and taking serum, and superoxide dismutase (SOD) test kit is purchased from Nanjing and builds up bio-engineering research institute, measures with microplate reader.
3. experimental result: experimental data is carried out statistical analysis.
Experimental result is as follows:
1. lipid peroxide catabolite malonaldehyde (MDA) assay in the serum
Table 2 trial drug is to lipid peroxide catabolite malonaldehyde (MDA) content influence in the mice serum
Compare with model group, * represents P＜0.05, and * * represents P＜0.01
2. superoxide dismutase (SOD) vitality test in the serum
Table 3 trial drug is to superoxide dismutase in the mice serum (SOD) effect of vigor
Compare with model group, * represents P＜0.05, and * * represents P＜0.01
Brief summary
Oral administration gives the compound preparation 30 days of mice various dose, can significantly improve superoxide dismutase in the mice serum (SOD) activity, significantly reduces malonaldehyde in the mice serum (MDA) activity.According to the regulation of " function of health food is learned assessment process and the method for inspection ", functional food of the present invention has anti-aging effects.
Can be found out that by experimental result the present invention is slow down aging obviously, can improve superoxide dismutase in the serum (SOD) activity, reduce malonaldehyde (MDA) activity in the serum, be antidotal ideal health care product.
Claims (2)
1. antidotal compound preparation of Cornu Cervi Pantotrichum, Radix Polygoni Multiflori, Fructus Lycii and Semen Sesami Nigrum is characterized in that mainly being made by ratio of weight and the number of copies by following material:
5 parts in Cornu Cervi Pantotrichum, 20 parts of Fructus Lyciis, 20 parts of Radix Polygoni Multiflori, 20 parts of Semen Sesami Nigrums.
2. the preparation technology of Cornu Cervi Pantotrichum, Radix Polygoni Multiflori, Fructus Lycii and the antidotal compound preparation of Semen Sesami Nigrum as claimed in claim 1 may further comprise the steps:
(1) Radix Polygoni Multiflori: Semen Sesami Nigrum is put into 60% ethanol that extractor adds 6 times of weight with 1: 1 ratio, and 65 ℃ of reflux, extract, 2 hours are extracted 2 times, with sieve elimination medicinal residues, merges the water extraction liquid of 2 extractions;
(2) Cornu Cervi Pantotrichum: Fructus Lycii is put into extractor with 1: 4 ratio and adds 7 times of weight water soakings 3 hours, and reflux, extract, 2 hours is extracted 3 times altogether, with sieve elimination medicinal residues, merges the water extraction liquid of 3 extractions;
(3) medicinal liquid of combining step (1) and step (2) is concentrated into 1/4 of cumulative volume, sucking filtration, and filtrate adds dehydrated alcohol to 50% determining alcohol to remove impurity, and room temperature leaves standstill 48h;
(4) medicinal liquid with step (3) carries out sucking filtration, and filtrate places the water-bath Back stroke to nothing alcohol flavor, sucking filtration, and filtrate adds dehydrated alcohol to 75% determining alcohol to remove impurity, cold preservation 48h;
(5) medicinal liquid behind step (4) precipitate with ethanol is carried out sucking filtration, filtrate places the water-bath Back stroke to nothing alcohol flavor, sucking filtration, and filtrate adds 2.5 times water, boils 10min, is cooled to room temperature, cold preservation 48h;
(6) medicinal liquid of extraction step (5) is diluted to 50mL in right amount; then add 500mg taurine, 500mg vitamin E, vitamin B2 5mg, vitamin B3 20mg, vitamin B6 5mg, adding sucrose 5mg, glycerol 0.5mL, potassium sorbate 20mg, polyvinylpyrrolidone 150mg, polyoxyethylene hydrogenated Oleum Ricini 1g, citric acid transfer pH to 3.8 to make oral liquid.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102688471A (en) * | 2012-06-18 | 2012-09-26 | 北华大学 | Compound preparation for preventing fatigue |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102688471A (en) * | 2012-06-18 | 2012-09-26 | 北华大学 | Compound preparation for preventing fatigue |
Non-Patent Citations (3)
| Title |
|---|
| 张有寯编: "《中国养生系列 7性养生与长寿》", 31 March 1994, 开今文化出版 * |
| 李永来: "《中华名方》", 31 January 2012, 黑龙江科学技术出版社 * |
| 顾奎琴等: "《美容养颜与饮食调养》", 31 January 1996, 科学普及出版社 * |
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Application publication date: 20130403 |