CN108813617B - A kind of ginseng wolfberry composition and preparation method thereof - Google Patents
A kind of ginseng wolfberry composition and preparation method thereof Download PDFInfo
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Abstract
A composition containing pilose antler, ginseng and medlar and a preparation method thereof belong to the field of health-care food of traditional Chinese medicine. Aiming at the problem of few types of antioxidant health care products which take traditional Chinese medicinal materials as main raw materials at present, the invention takes pilose antler as the main raw material, and red ginseng, astragalus, medlar, schisandra chinensis and Chinese date as auxiliary materials, and the pilose antler, ginseng and medlar composition is prepared after processing, and the composition can delay senility, improve the oxidation resistance of organisms, and can be prepared into health care products such as tea drinks and the like.
Description
Technical Field
The invention relates to the field of traditional Chinese medicine health-care food, in particular to an antler-ginseng-wolfberry composition and a preparation method thereof.
Background
Various free radicals are continuously generated in the oxidative metabolism process of life activities, wherein superoxide anion free radicals
Hydroxyl radicals (. OH) and aliphatic radicals (ROO. cndot.) are three representative radicals.
The earliest production, OH, was the most potent. Freedom of movementThe generation of the gene is closely related to the occurrence of a plurality of functional disorders and diseases of the body, such as phagocytosis, detoxification, inflammation, tumor, aging, radiation and the like. Free radicals cause lipid peroxidation of biological membranes, resulting in damage to membrane structure and functional inactivation. Many substances in biological systems provide electrons to molecular oxygen through autooxidation, which is one of the sources for generating oxygen radicals. Free radical reactions spread to cause damage to biological macromolecules, causing damage to the body. The body also forms an enzyme system that protects against free radicals, such as superoxide dismutase, glutathione peroxidase, catalase, etc. There is also a protective system, i.e. antioxidants or radical scavengers, such as ascorbic acid, cysteine, glutathione, etc., which are widely present in the intracellular and extracellular aqueous phases. Under physiological conditions, free radicals are continuously generated and continuously removed, so that favorable, harmless, physiologically low-level and stably balanced free radical concentration is maintained. In certain pathological conditions, the production and clearance of free radicals are out of balance.
The free radical can initiate the peroxidation of unsaturated fatty acid arachidonic acid of biological membrane to form lipid PG peroxideS、LTSAnd MDA and other metabolic products. MDA is the degradation product of lipid peroxide, and can be combined with protein and nucleic acid containing free amino group to crosslink biomolecules to form lipofuscin or senile pigment. The substance exists in tissues such as brain, heart, liver cell and epidermis, and is increased remarkably with aging, so that protein is denatured, solubility is reduced, and membrane structure and function are damaged. Research proves that the free radical lipid peroxidation damage is closely related to common senile diseases such as atherosclerosis, cerebrovascular diseases, myocardial infarction, pulmonary hypertension, diabetes, cataract, autoimmune diseases, liver damage and the like. A large number of documents report that low density lipoproteins are modified by free radical oxidation, resulting in the formation of arteriosclerosis. PG (Picture experts group)S、LTSIs a metabolite of in vivo unsaturated fatty acid-arachidonic acid respectively passing through cyclooxygenase and lipoxygenase pathways, and has close relation with inflammation, myocardial infarction, cerebral hemorrhage, cerebral edema, cerebral vasospasm, subretinal hemorrhage, pulmonary hypertension and the like. Combining the above-mentioned damage of free radicals to human bodyThe preparation of antioxidant health care products for removing free radicals is a hot point at home and abroad.
At present, the antioxidant health care products prepared from traditional Chinese medicinal materials in the market are few in variety. Therefore, the health care product prepared from the traditional Chinese medicinal materials has higher market value.
Disclosure of Invention
Aiming at the problem that the types of the antioxidant health care products taking traditional Chinese medicinal materials as main raw materials are few at present, the invention provides an antler-ginseng-wolfberry composition and a preparation method thereof.
The main raw materials of the antler-ginseng-wolfberry composition are extracts of antler, red ginseng, astragalus, wolfberry, schisandra and jujube.
Further, the antler, ginseng and medlar composition is composed of extracts of the following raw materials in parts by weight: 14-16 parts of pilose antler, 19-21 parts of red ginseng, 14-16 parts of astragalus root, 23-25 parts of medlar, 13-15 parts of Chinese date and 11-13 parts of Chinese magnoliavine fruit.
Preferably, the antler, ginseng and medlar composition consists of extracts of the following raw materials in parts by weight: 16 parts of pilose antler, 21 parts of red ginseng, 14 parts of astragalus, 25 parts of medlar, 13 parts of Chinese date and 11 parts of Chinese magnoliavine fruit.
The invention also provides a preparation method of the antler-ginseng-wolfberry composition, which comprises the following steps of weighing the antler, the red ginseng, the astragalus, the wolfberry, the schisandra chinensis and the Chinese date according to parts by weight, preparing extracts of the raw materials, and mixing the extracts to obtain the antler-ginseng-wolfberry composition, wherein the preparation method of the extracts of the raw materials comprises the following steps:
1) extracting cornu Cervi Pantotrichum with ultrasonic wave to obtain extractive solution, filtering to obtain clear liquid, subjecting the clear liquid to enzymolysis with protease and pancreatin to obtain enzymatic hydrolysate, concentrating, and drying to obtain cornu Cervi Pantotrichum extract;
2) extracting Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae respectively with ethanol under reflux to obtain extractive solutions, filtering, mixing filtrates, concentrating the filtrate, and drying to obtain extract mixture of Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae;
3) decocting fructus Jujubae in water, pulping, soaking in water, extracting with ultrasonic wave, filtering the obtained extractive solution, performing enzymolysis, leaching, filtering again, concentrating the filtrate, and drying to obtain fructus Jujubae extract.
Further limiting, the concentration in the steps 1) to 3) is vacuum concentration, the concentration condition is 60 ℃, and the concentration is 0.06-0.08MPa, and the concentration is carried out until the relative density is 1.10-1.15.
Further, the drying in the steps 1) to 3) is realized by spray drying, the inlet temperature is 160-180 ℃, and the outlet temperature is 60-80 ℃.
Further limiting, the mass ratio of the protease to the pancreatin in the step 1) is 1: 1; step 2), refluxing the ethanol for 2 times, and extracting for 3 hours; step 3) adding water to pre-cook the Chinese dates, namely cooking the Chinese dates in water for 30-40min at 100 ℃; the ultrasonic extraction power is 150W, the extraction time is 3h, the enzymolysis is adding pectinase with 0.2% of the volume of the filtrate, and the enzymolysis extraction is carried out for 6h at the temperature of 45 ℃.
The antler, ginseng and medlar composition can be processed into health care products of different formulations, such as tablets, beverages and the like, together with other additives or auxiliary materials.
Advantageous effects
Pilose antler is sweet and warm, mainly leaks and has aversion to blood, cold and heat convulsions and pain, tonifies qi and strengthens mind, and produces teeth not old. Antler mainly deteriorates, wounds, carbuncle and swelling, and dispels evil and evil qi. Cornu Cervi Pantotrichum has effects of replenishing essence and marrow, warming kidney deficiency and cold, nourishing bone and tonifying yang, strengthening tendons and bones, and resisting aging after long-term administration, and Os Cervi has effects of replenishing bone and dispelling pathogenic wind, and can resist aging after long-term administration. Modern researches have proved that pilose antler has wide pharmacological actions, wherein the functions of accelerating wound healing, promoting bone repair and the like are consistent with the records in the above documents. The cornu Cervi Pantotrichum and Os Cervi are rich in bioactive components, and calcium and phosphorus are one of the best sources of biological calcium and phosphorus.
Ginseng is sweet in taste and slightly cold in nature, has the effects of tonifying five internal organs, calming spirit, stopping palpitation, eliminating pathogenic qi, benefiting heart and intelligence, losing weight and prolonging life after long-term taking. The red ginseng is one of processed products of ginseng, and is named as red ginseng, wherein fresh ginseng is steamed for 2-3 hours and becomes dark red in internal and external colors after being baked and dried. After the red ginseng is treated at high temperature, the content of ginsenoside, amino acid and the like is reduced, but the content of argininyl-fructosyl is increased, and maltol and other components are newly generated, so that the red ginseng has stronger effects of resisting oxidation and aging, enhancing immunity, dilating blood vessels and the like. Meanwhile, after being steamed, the red ginseng has warm medicinal properties, is better than greatly tonifying primordial qi, tonifying qi and controlling blood, and is suitable for patients with extreme qi deficiency, weak pulse, heart failure and cardiogenic shock.
Astragalus root, radix astragali, sweet in flavor and slightly warm in nature, is mainly used for treating carbuncle and deep-rooted carbuncle, which is long-term in decay, wound, pus discharge and deficiency tonifying. The schisandra chinensis is sour and warm, mainly tonifies qi, hurts, shortages, weak, tonifies deficiency, strengthens yin and benefits male essence. Radix astragali is root of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao of Leguminosae. Light smell, slightly sweet taste and beany flavor. Contains calycosin (calycosin) and 3-hydroxy-9, 10-dimethoxy rosewood alkane as flavone components, and also contains astragalosides I, V and III. Warm in nature and sweet in taste. Radix astragali has effects of invigorating qi, invigorating yang, consolidating superficial resistance, arresting sweating, inducing diuresis, relieving swelling, removing sore, and promoting granulation. Pharmacological experiments show that astragalus has the effects of tonifying heart, promoting urination, reducing blood pressure, protecting liver and improving immune function. Dilating blood vessels, improving the blood circulation of skin and having good nutrition. ③ has a certain eliminating function to the proteinuria of nephritis. Is suitable for deficiency of vital energy, asthenia, spontaneous perspiration, and common cold; collapse due to deficiency of middle-jiao, anorexia, loose stool, gastroptosis, rectocele, hematochezia, female metroptosis, metrorrhagia; sores and abscess with pus forming and no ulceration or ulceration for a long time; edema due to qi deficiency and all qi deficiency syndromes.
Medlar is bitter and cold in flavor, mainly treats pathogenic factors in five internal organs, dissipates heat, quenches thirst, and is astringent, hard to strengthen tendons after being taken for a long time, light and not old. The medlar has the effects of tonifying the kidney and replenishing vital essence, nourishing the liver and improving eyesight, enriching the blood and soothing the nerves, promoting the production of body fluid to quench thirst, moistening the lung and relieving cough. It can be used for treating yin deficiency of liver and kidney, soreness of waist and knees, dizziness, blurred vision, hyperdacryosis, cough due to asthenia, diabetes, and spermatorrhea. The efficacy of the medlar is highly advocated by doctors of all ages, and the traditional Chinese medicine considers that the medlar has the effects of prolonging life and delaying senility after being taken for a long time. Fructus Lycii has effects of improving immunity, invigorating qi, replenishing essence, nourishing liver and kidney, resisting aging, quenching thirst, warming body, and resisting tumor. Fructus Lycii has effects of lowering blood pressure, blood lipid and blood sugar, preventing atherosclerosis, protecting liver, resisting fatty liver, and promoting liver cell regeneration. Modern medicine believes that medlar has the following ten health-care effects: 1. the fructus Lycii has effects of enhancing specific and nonspecific immunity, and regulating immunity. 2. Fructus Lycii has anti-tumor effect. 3. Fructus Lycii has antioxidant and antiaging effects. 4. Fructus Lycii has effects of protecting liver and resisting fatty liver. 5. The medlar can stimulate the growth of organisms and has obvious protective effect on genetic injuries induced by certain genetic toxicants. 6. Fructus Lycii has effect in promoting hematopoiesis. 7. The medlar can influence the function of the hypothalamus-pituitary-gonad axis and has good effect of reducing blood sugar. 8. The fructus Lycii can enhance reproductive system function, and enhance contraction frequency, tension and strength of in vitro uterus. 9. The medlar can increase the content of hydroxyproline in the skin of the mouse, obviously enhance the hypoxia tolerance of the mouse, prolong the swimming time of the mouse and resist fatigue. 10. The fructus Lycii has effect in lowering blood pressure. Medlar contains rich carotene, vitamins A1, B1, B2, C and other calcium, iron and other necessary nutrients for healthy eyes, so the medlar is good at improving eyesight, and is commonly called as 'eyesight improving eyes'. Lycium chinense is often used by doctors of all generations to treat blurred vision and night blindness caused by liver-blood deficiency and kidney-yin deficiency. The well-known prescription, QIJUDIHUANG pill, uses fructus Lycii as the main drug. The Chinese wolfberry is also commonly used for treating chronic eye diseases in folks, and the Chinese wolfberry steamed egg is a simple and effective dietary therapy formula. The traditional Chinese medicine holds that the health-care tea can nourish liver and kidney, replenish vital essence to improve eyesight, nourish blood and enhance immunity of people. For modern people, the most practical effects of wolfberry are fatigue resistance and blood pressure reduction. In addition, the medlar can protect the liver, reduce the blood sugar, soften the blood vessel, reduce the cholesterol and triglyceride level in the blood, and has certain curative effect on fatty liver and diabetes patients. According to clinical medical verification, the medlar can also treat chronic renal failure.
Wu Wei Zi astringes and astringes, tonifies qi and generates body fluid, tonifies kidney and calms heart. Its fruit is known for its five tastes of sweet, sour, pungent, bitter and salty, and has the north-south scores. Li Shizhen is said that "five flavors have north and south scores at present, south produces red color, north produces black color, and it is good for north produces when used as tonic. "northern schisandra fruit, also called zanthoxylum bungeanum, is an environment with magnolia, which is a perennial twining vine, cold resistant, pleased with cool and damp and humus soil. The fructus Schisandrae seed contains volatile oil. The oil contains citral, alpha, beta-thujene, and beta-thujaaldehyde as main ingredients. The fruit contains citric acid, malic acid, tartaric acid, and Vc. Seeds contain a large amount of fatty acids. Has effects in astringing, arresting discharge, invigorating qi, promoting salivation, invigorating kidney, and calming heart. According to the records of this Jing, Wu Wei Zi is used to supplement qi, tonify deficiency, strengthen yin and benefit male essence. Schisandrin B and schisanhenol have antioxidant effect, and can scavenge free radicals and inhibit formation of lipid peroxide. The schisandra fruit water extract for stomach irrigation of old mice can obviously inhibit the activity of MAO-B in brain and liver, enhance the activity of SOD and reduce the content of MDA. Schisanhenol can directly resist heart mitochondrial toxicity caused by adriamycin, and inhibit lipid peroxidation of brain, liver and kidney microsomes and mitochondria of rats induced by vitamin C-NADPH, and has stronger action than vitamin E. Can also obviously improve the content of cAMP in myocardial cells of the aged rats, increase the ratio of cAMP/cGMP and enhance the heart activity. In addition, the schisandra chinensis can reduce serum cholesterol and increase the protein content in the brain and liver, which shows that the schisandra chinensis has an anti-aging effect.
Jujube, namely dry jujube, beautiful jujube, good jujube (entry of medical science), red jujube (entry of medicine), mature fruit of jujube of Rhamnaceae, jujube saponin I, jujube saponin II, jujube saponin III (ziziziphussaponin I, jujube saponin II, jujube seed saponin B (jujujuuboside B), stephanine (stephanine), glucose, fructose, sucrose, adenosine cyclophosphate (cAMP), cGMP (cGMP), and the like, sweet in nature and taste, warm, Benjing, sweet in taste, flat, Qianjin food treatment, sweet in taste, hot, nontoxic, Meng Yang, in spleen and stomach channels, compendium, spleen channel blood, Ben Cao Jing Shu, Zu Yin, Yang Ming channel, tonifying spleen and stomach, recuperating ying wei, relieving drug toxicity, treating deficiency of stomach, weak spleen, weak stool, blood, weak spleen and kidney-yang, nourishing spleen, kidney-yin, kidney-yang, spleen and kidney-yang, unblocking nine orifices, tonifying qi and body fluids, deficiency of the body and middle energizer, severe convulsion, heaviness of limbs, and combining hundreds of herbs. ② Ben Cao Jing Ji Zhu: the poison of Aconitum carmichaeli Debx. ③ Bian Lu: tonify middle-jiao and Qi, strengthen the body, remove vexation, treat the suspended heart and the isolated bowel . Fourthly, medicine Pair: killing aconite root and Tianxiong poison. Fifth, washing: mainly tonify body fluid, wash heart and abdomen pathogenic qi, neutralize all kinds of drug toxicity, dredge nine orifices, tonify insufficient qi, cook and tonify intestines and stomach, nourish middle-jiao and qi for the first time, so children suffer from autumn dysentery, and eat well with Chongzao. Sixthly, the book of Japanese Huazi materia Medica: moisten heart and lung, and relieve cough. Tonify five internal organs, treat deficiency and strain, and remove qi of intestine and stomach. Seventhly, pearl sac: warming the stomach. Li Gao: warm to tonify spleen meridian, sweet to relieve yin and blood, harmonize yin and yang, regulate ying and wei, and promote the production of body fluid. Ninthly, pharmaceutical meaning: nourish blood and tonify liver. R "materia medica renewal": tonify middle energizer and qi, nourish kidney and warm stomach, treat yin deficiency. The drawing refers to the Chinese medicine plantation: treating allergic purpura, anemia and hypertension.
The pilose antler-ginseng-wolfberry fruit composition is prepared by taking pilose antler as a main raw material, red ginseng, astragalus, wolfberry fruit, schisandra fruit and Chinese date as auxiliary materials, adopting monarch, minister, assistant and guide medicines, dialectical treatment and mutual promotion and taboo principles, taking pilose antler as a monarch medicine, red ginseng and wolfberry fruit as minister medicines, astragalus and schisandra fruit as assistant medicines and Chinese date as a conductant medicine and applying the characteristics of multiple Chinese herbal medicine components and complex functions, reasonably matching and extracting extracts of all raw materials2) The ginseng and medlar composition also has obvious clearing effect, and shows that the ginseng and medlar composition has good antioxidation and aging delaying effect.
Detailed Description
The antler-ginseng-wolfberry composition described in the following embodiments is commercially available from the main raw material of spotted deer antler and the auxiliary materials of red ginseng, astragalus, wolfberry, schisandra and jujube, and the extracts are obtained by processing the raw materials and mixing the extracts to obtain the antler-ginseng-wolfberry composition.
The pectinase is in powder form and purchased from: product number of bio-engineering (Shanghai) Ltd: A605099.
the protease was in powder form and purchased from: beijing Solaibao science and technology Limited, the product number is: B8361.
the pancreatin was in powder form and purchased from: biometrics (Shanghai) Inc.; the goods number is: A606787.
example 1. composition of pilose antler, ginseng and wolfberry fruit.
The antler, ginseng and medlar composition consists of extracts of the following raw materials in parts by weight: 16 parts of pilose antler, 21 parts of red ginseng, 14 parts of astragalus, 25 parts of medlar, 13 parts of Chinese date and 11 parts of Chinese magnoliavine fruit.
1) The preparation method of the pilose antler extract comprises the following steps: selecting a person with no putrefaction odor and deterioration from dried cornu Cervi Pantotrichum, burning with alcohol to remove cornu Cervi Pantotrichum, cleaning, adding 3 times of water, decocting with water at 80 deg.C for 10-15 min, peeling, decocting for about 10min, and slicing or crushing. Extracting with 40% (v/v) ethanol (20 mL/g) under ultrasonic wave (150W, 40min) at room temperature. Filtering to obtain clear liquid, recovering ethanol under reduced pressure, and adding protease and pancreatin mixture with a mass ratio of protease to pancreatin of 1:1, wherein the volume of the protease and pancreatin mixture is 1.5% (mass mg/volume 100 mL); the enzymolysis time is 6h, the enzymolysis temperature is 60 ℃, macromolecular proteins in the clear liquid are degraded to polypeptide, and then spray drying (inlet temperature is 160 ℃, outlet temperature is 60 ℃) is carried out when the relative density is 1.10 after vacuum concentration (60 ℃, 0.06MPa) is carried out, so as to obtain spray-dried fine powder I, namely the pilose antler extract.
2) The preparation method of the red ginseng, astragalus, medlar and schisandra extract comprises the following steps: removing impurities from Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae chinensis, slicing or crushing, cleaning, draining, reflux-extracting with 10 times of 70% ethanol for 2 times, each for 3 hr; filtering, mixing filtrates, and recovering ethanol under reduced pressure. Vacuum concentrating the filtrate (60 deg.C, 0.06MPa) to relative density of 1.10, and spray drying (inlet temperature of 160 deg.C and outlet temperature of 60 deg.C) to obtain spray dried fine powder II, which is mixture of Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae extract.
3) The preparation method of the Chinese date extract comprises the following steps: selecting fully mature red dates (dry and fresh), removing impurities, cleaning, adding 2 times of water by mass, placing in a jacketed pan, pre-boiling at 100 ℃ for 30min, removing cores manually or mechanically, pulping pulp by a pulping machine, adding 8 times of water by volume of pulp, soaking for 30min, ultrasonically extracting (150W,3h), filtering, adding pectinase powder according to the proportion of 0.2% of filtrate (namely 0.2mg of pectinase powder is added into 100mL of filtrate), carrying out heat preservation and enzymolysis extraction at 45 ℃ for 6h, heating to 60 ℃ for enzyme deactivation, cooling to 40-30 ℃ for filtering, carrying out vacuum concentration (60 ℃, 0.06MPa) to relative density of 1.10, carrying out spray drying (inlet temperature of 160 ℃ and outlet temperature of 60 ℃) to obtain spray-dried fine powder III, namely the jujube extract.
Mixing the dried fine powder prepared in the steps to obtain the antler-ginseng-medlar composition.
Example 2. the composition of ginseng and wolfberry fruit according to the present embodiment is composed of the following extracts of raw materials in parts by weight: 15 parts of pilose antler, 20 parts of red ginseng, 15 parts of astragalus, 24 parts of medlar, 14 parts of Chinese date and 12 parts of Chinese magnoliavine fruit.
1) The preparation method of the pilose antler extract comprises the following steps: selecting a person with no putrefaction odor and deterioration from dried cornu Cervi Pantotrichum, burning with alcohol to remove cornu Cervi Pantotrichum, cleaning, adding 3 times of water, decocting with water at 80 deg.C for 10-15 min, peeling, decocting for about 10min, and slicing or crushing. Extracting with 40% (v/v) ethanol (20 mL/g) under ultrasonic wave (150W, 40min) at room temperature. Filtering to obtain clear liquid, recovering ethanol under reduced pressure, and adding protease and pancreatin mixture with a mass ratio of protease to pancreatin of 1:1, wherein the volume of the protease and pancreatin mixture is 1.5% (mass mg/volume 100 mL); the enzymolysis time is 6h, the enzymolysis temperature is 60 ℃, macromolecular proteins in the clear liquid are degraded to polypeptide, and then spray drying (inlet temperature is 160 ℃, outlet temperature is 60 ℃) is carried out when the relative density is 1.10 after vacuum concentration (60 ℃, 0.06MPa) is carried out, so as to obtain spray-dried fine powder I, namely the pilose antler extract.
2) The preparation method of the red ginseng, astragalus, medlar and schisandra extract comprises the following steps: removing impurities from Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae chinensis, slicing or crushing, cleaning, draining, reflux-extracting with 10 times of 70% ethanol for 2 times, each for 3 hr; filtering, mixing filtrates, and recovering ethanol under reduced pressure. Vacuum concentrating the filtrate (60 deg.C, 0.06MPa) to relative density of 1.10, and spray drying (inlet temperature of 160 deg.C and outlet temperature of 60 deg.C) to obtain spray dried fine powder II, which is mixture of Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae extract.
3) The preparation method of the Chinese date extract comprises the following steps: selecting fully mature red dates (dry and fresh), removing impurities, cleaning, adding 2 times of water by mass, placing the red dates in a jacketed pan, pre-boiling at 100 ℃ for 30-40min, removing kernels manually or mechanically, pulping the pulp by using a pulping machine, adding 8 times of water by volume of pulp, soaking for 30min, ultrasonically extracting (150W,3h), filtering, adding pectinase powder according to the proportion of 0.2% of filtrate (namely 0.2mg of pectinase powder is added into 100mL of filtrate), carrying out heat preservation and enzymolysis extraction at 45 ℃ for 6h, heating to 60 ℃ to inactivate enzyme, cooling to 40-30 ℃ to filter, carrying out vacuum concentration (60 ℃, 0.06MPa) to relative density of 1.10, and carrying out spray drying (inlet temperature of 160 ℃ and outlet temperature of 60 ℃) to obtain spray-dried fine powder III, namely the jujube extract.
Mixing the dried fine powder prepared in the steps to obtain the antler-ginseng-medlar composition.
Example 3 the difference between this example and example 1 is that the composition of pilose antler, ginseng and wolfberry fruit described in this example is composed of the following extracts of raw materials in parts by weight: 14 parts of pilose antler, 21 parts of red ginseng, 14 parts of astragalus, 25 parts of medlar, 13 parts of Chinese date and 13 parts of Chinese magnoliavine fruit.
The preparation method of the composition is the same as that of example 1.
Research on scavenging free radicals of antler, ginseng and medlar composition:
taking the antler, ginseng and medlar composition prepared in example 1 as an example, the effect of the antler, ginseng and medlar composition of the invention in scavenging free radicals is examined.
1 preparing an extracting solution.
Adding distilled water with the mass being 12 times of that of 20g of the composition of the pilose antler, the ginseng and the medlar, heating and dissolving the mixture into suspension, putting the suspension into an ultrasonic extractor for 3 times at the ultrasonic frequency of 18KHZ, extracting the suspension for 3 times at the ultrasonic dose of 60min (1h), combining the extracting solutions, concentrating the mixture in a water bath, centrifuging the mixture for 15min at 3000rpm, taking supernate to prepare aqueous solution with the solid content of 1000 mu g/mL, and adding water to dilute the aqueous solution into solutions with the solid content of 40 mu g/mL, 60 mu g/mL, 80 mu g/mL, 100 mu g/mL, 120 mu g/mL and 140 mu g/mL respectively for standby.
2 the composition of pilose antler, ginseng and medlar has the function of eliminating hydroxyl free radicals.
Taking 1.0mL of phosphate buffer solution with the pH value of 7.4, 0.2mL of pansafflower red (500 mu g/mL) solution and EDTANA2－Fe2+1.0mL of the solution, respectively adding 7.0mL of the antler-ginseng-medlar composition solution with different concentrations, and finally adding 6% H by mass fraction2O20.8mL, mixing, keeping the temperature in a water bath at 40 ℃ for 30min, and measuring the absorbance A value at the wavelength of 520 nm. The blank group replaced the sample solution with an equal volume of distilled water; the control group replaced the sample solution and EDTA with equal volume of distilled waterNa2－Fe2+And (3) solution. The clearance (E%) was calculated as follows. The results are shown in Table 1, using mannitol and benzoic acid as controls.
EC50Indicating the concentration of the sample at which the clearance rate reached 50%.
TABLE 1 Effect of Rong, Shen and Gou Qi composition on OH (hydroxyl radical) scavenging
3 scavenging effect of antler, ginseng and medlar composition on superoxide anion free radicals
The scavenging effect of the antler, ginseng and medlar composition on superoxide anion free radicals generated by the antler, ginseng and medlar composition is measured by using pyrogallol autoxidation reaction. The solutions of the reaction systems were prepared as listed in Table 2, with pyrogallol added last. When the pyrogallol solution is added, the solutions of all reaction systems are quickly and uniformly mixed, and the maximum value of absorbance, namely the reaction rate (autoxidation rate), is measured at the wavelength of 320 nm. The clearance E% was calculated as follows. E% ((S))Blank space－SSample (I))×100/SBlank spaceAnd S is the autoxidation rate.
TABLE 2 composition of each reaction system solution
Firstly, measuring the absorption spectrum of pyrogallol autoxidation system, determining autoxidation rate, namely maximum absorbance value SBlank spaceAnd maximum absorption wavelength (320 nm), and measuring maximum absorbance value S after adding into the aqueous solution of the composition of pilose antler ginseng and medlar at 320nm wavelengthSample (I)(autoxidation rate) was compared to Vc. Calculating E% according to the formula. The results are shown in Table 3.
TABLE 3 scavenging effect of Rong Shen Gou Qi composition on superoxide anion radical
Experiments prove that the product can obviously eliminate hydroxyl free radical (. OH) and superoxide anion free radical
Has strong antioxidation, can be used as a nutrition health care product for resisting oxidation and delaying senility and serves the consumers.
Claims (1)
1. A composition containing cornu Cervi Pantotrichum, Ginseng radix and fructus Lycii is prepared from extracts of cornu Cervi Pantotrichum, Ginseng radix Rubri, radix astragali, fructus Lycii, fructus Schisandrae chinensis and fructus Jujubae; the antler, ginseng and medlar composition consists of extracts of the following raw materials in parts by weight: 16 parts of pilose antler, 21 parts of red ginseng, 14 parts of astragalus, 25 parts of medlar, 13 parts of Chinese date and 11 parts of Chinese magnoliavine fruit; the preparation method of the antler-ginseng-wolfberry composition comprises the following steps of weighing antler, red ginseng, astragalus mongholicus, wolfberry, schisandra chinensis and jujube according to parts by weight, preparing extracts of the raw materials, and mixing the extracts to obtain the antler-ginseng-wolfberry composition, wherein the preparation method of the extracts of the raw materials comprises the following steps:
1) extracting cornu Cervi Pantotrichum with ultrasonic wave to obtain extractive solution, filtering to obtain clear liquid, subjecting the clear liquid to enzymolysis with protease and pancreatin to obtain enzymatic hydrolysate, concentrating, and drying to obtain cornu Cervi Pantotrichum extract; the mass ratio of the protease to the pancreatin is 1:1, the enzymolysis time is 6h, and the enzymolysis temperature is 60 ℃; the power of the ultrasonic wave is 150W, and the extraction time is 40 min;
2) extracting Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae respectively with ethanol under reflux to obtain extractive solutions, filtering, mixing filtrates, concentrating the filtrate, and drying to obtain extract mixture of Ginseng radix Rubri, radix astragali, fructus Lycii and fructus Schisandrae; refluxing the ethanol for 2 times, and extracting for 3 hours;
3) pre-cooking fructus Jujubae in water, pulping, soaking in water, extracting with ultrasonic wave, filtering the obtained extractive solution, performing enzymolysis, leaching, filtering again, concentrating the filtrate, and drying to obtain fructus Jujubae extract; the pre-cooking of the Chinese dates with water is to cook the Chinese dates for 30-40min at 100 ℃; the power of ultrasonic extraction is 150W, and the extraction time is 3 h; the enzymolysis is to add pectinase with 0.2 percent of the volume of the filtrate, and carry out enzymolysis and extraction for 6 hours at the temperature of 45 ℃;
the concentration in the steps 1) -3) is vacuum concentration, the concentration condition is 60 ℃, the concentration is 0.06-0.08Mpa, and the concentration is carried out until the relative density is 1.10-1.15; the drying in the steps 1) -3) is spray drying, the inlet temperature is 160-180 ℃, and the outlet temperature is 60-80 ℃.
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