CN103004460A - Growth bottle lid for grifola frondosa cultivation - Google Patents

Growth bottle lid for grifola frondosa cultivation Download PDF

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Publication number
CN103004460A
CN103004460A CN2011102988572A CN201110298857A CN103004460A CN 103004460 A CN103004460 A CN 103004460A CN 2011102988572 A CN2011102988572 A CN 2011102988572A CN 201110298857 A CN201110298857 A CN 201110298857A CN 103004460 A CN103004460 A CN 103004460A
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China
Prior art keywords
bottle cap
base portion
cultivation
millimeters
type device
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CN2011102988572A
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Chinese (zh)
Inventor
程继红
张硕
杨仁智
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SHANGHAI FINC BIO-TECH Inc
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SHANGHAI FINC BIO-TECH Inc
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Priority to CN2011102988572A priority Critical patent/CN103004460A/en
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Abstract

The invention relates to a growth bottle lid for grifola frondosa cultivation. The growth bottle lid is made of an opaque material, comprises a base part and a funnel-shaped device arranged on the base part and is arranged on an opening of a culture bottle through the base part in a covering way, the funnel-shaped device is communicated with the base part through a primordium growth hole and comprises a bud forming device and a connection part, the connection part is connected with the bus forming device and the base part, the inside diameter D of the base part is more than or equal to 56mm and less than or equal to 64mm, the height H1 of the funnel-shaped device is more than or equal to 5mm and less than or equal to 20mm, and the height H2 of the connection part is more than or equal to 1.6mm and less than or equal to 3mm.

Description

The grifola frondosus cultivation is with giving birth to bottle cap
Technical field
The present invention relates to bottle and plant Cultivation of Grifola frondosa, or rather, relate to a kind of grifola frondosus cultivation with giving birth to bottle cap.
Background technology
Grifola frondosus is a kind of food, medicine dual-purpose gill fungus bacterium, belongs to Basidiomycotina, Hymenomycetes, Septobasidium sp subclass, Aphyllophorales, Polyporaceae, tree flower Pseudomonas fungi.Have another name called polyporus frondosus, chestnut mushroom, cloud gill fungus etc.Fruit body is by overlapping the forming of cap of stem and a spoon shape, fan-shaped or the ligule of branch repeatedly, the wide 2-7 of cap centimetre; Existing domestic cultivar is take brown as main.Bacterial context white, thick 1-3 millimeter; Pore prolongs life, mouth of pipe polygonal.Spore is colourless, and oval is to oval.
Grifola frondosus is not still carried out the report that batch production is produced at China at present, and in the Qianxi in Hebei, some seasonal cultivations have appearred in the ground such as the Qingyuan in Zhejiang, plastic sack with plantation xianggu stick type carries out pseudo-wild cultivating, and the first damp mushroom is imbedded after rolling the underground soil covering culture that carries out and goes out the second damp mushroom, and this kind cultivation mode is comparatively extensive, can not year-round supply, Grifola Frondosa sporophore by the earthing output is often with a large amount of sands, and extremely difficult the cleaning brings risk for the quality of grifola frondosus.
Japan's plantation grifola frondosus has had the history of three more than ten years, has realized the production of batch production, but is main pocket type cultivation mainly with 2.5 kilograms of (wet feed loading) plastic sacks greatly, basic technology is for stirring pack, sterilization, cultivate and sprout, fertility, the step such as gather.Owing to being the plastic bag cultivation pattern, each sack can only use once, can't reuse; Secondly the sack filling machine that is associated with plastic sack, the pocket type inoculation device, carrying implement is all former because of not moulding of softness of plastic sack etc. thereby the difficulty of mechanization is multiplied, and cost of equipment is expensive, but the efficient of equipment only be bottle formula cultivation 1/20 or lower, plastic sack is often punctured by sharp thing when daily carrying and causes potential contamination hazard simultaneously.When plastic bag cultivation occurs at former base, need at ventilative filter membrane place with manually carrying out in a large number " people " font scarfing technique to impel grifola frondosus from bag, to grow, cause thus a large amount of costs of labor, and workman's technical ability is had larger dependence.2.5 being the 400-700 gram, do not wait the grifola frondosus average yield of kilogram pocket type cultivation output, the packing that really comes into the market to sell then is 100 grams, 150 grams or 200 grams, think that the output that reaches market standard needs again to carry out a large amount of dismountings and weighing operation, make troubles for the qualitative control of grifola frondosus.
In recent years, there is the grower to develop the bottle formula cultivation that the culture bottle that uses the wide-mouth bottle shape carries out grifola frondosus.Generally the diameter holes that the former base of Grifola Frondosa sporophore forms can be set in the central authorities of culture bottle lid, in the cultural hypha process, fruit body primordium is the projecting shape growth from this diameter holes, the culture bottle lid adopts light transmissive material to make, there are the following problems for this planting type: (1) is because in the uncovered placement of growing stage culture bottle, and be subject to light induction, can continue to have former base to form at growing stage, and the former base that this stage forms also can't develop into effective fruit body substantially, waste to a certain extent the nutrition of medium, and then affected the quality of individual plant grifola frondosus; (2) because in the uncovered placement of growing stage culture bottle, so that whole former base is exposed in the air, this can cause former base to begin again to disperse growth, grown again by originally " tangible " and to be " invisible " state, Grifola Frondosa sporophore outward appearance identity property is relatively poor when often causing the later stage to gather, and " flower " is not of uniform size.
Summary of the invention
The technical problem that the present invention solves is to overcome the defective that prior art exists, provide a kind of grifola frondosus cultivation with giving birth to bottle cap, use this fertility bottle cap to carry out the cultivation of grifola frondosus, avoided to a certain extent the waste of medium nutrition at growing stage, outward appearance identity property after the grifola frondosus maturation is significantly improved, and significantly promoted simultaneously the blade differentiation rate of grifola frondosus, effectively improved the grifola frondosus quality.
The present invention is achieved by the following technical solutions: a kind of grifola frondosus cultivation is with giving birth to bottle cap, described fertility bottle cap is made by light-proof material, comprise base portion and be arranged at the funnel type device of described base portion top, described fertility bottle cap adds by described base portion and is placed on the culture bottle mouth, described funnel type device grows the hole with base portion by former base and is communicated with, described funnel type device comprises mushroom flower bud former and connects the connecting portion of mushroom flower bud former and base portion, the internal diameter of described base portion satisfies 56 millimeters≤D≤64 millimeters, the height of described funnel type device satisfies 5 millimeters≤H1≤20 millimeters, and the height of described connecting portion satisfies 1.6 millimeters≤H2≤3 millimeters.
Described funnel type device and described base portion are one-body molded.
Described funnel type device and base portion link together removably.
Compared with prior art, by giving birth to bottle cap in growing stage the application of the invention, make culture bottle almost no longer produce former base at growing stage, improved the availability of medium nutrition, and by structure and the dimensional parameters of fertility bottle cap are controlled, the conforming while of outward appearance after improving the grifola frondosus maturation, significantly promoted the blade differentiation rate of grifola frondosus at growing stage, thereby promoted the quality of grifola frondosus.
Description of drawings
Fig. 1 is the schematic perspective view that the present invention gives birth to bottle cap.
Fig. 2 is the schematic perspective view that the present invention gives birth to another angle of bottle cap.
Fig. 3 is the cross-sectional schematic of Fig. 1.
Fig. 4 is for using the present invention to give birth to the perspective exploded view of the cultivation bottle cap that uses in the cultivation method of bottle cap.
Fig. 5 is for using the present invention to give birth to the perspective exploded view of the main part of the cultivation bottle cap that uses in the cultivation method of bottle cap.
Embodiment
See also Fig. 1, Fig. 2 and shown in Figure 3, the present invention gives birth to bottle cap 2 and is made by light-proof material, comprise base portion 20 and be arranged at the funnel type device 21 of base portion 20 tops, described funnel type device 21 grows hole 22 with base portion 20 by the former base that arranges and is communicated with, described funnel type device 21 comprises mushroom flower bud former 210 and connects the connecting portion 211 of mushroom flower bud former 210 and base portion 20, be provided with some stuck points 202 on the medial surface 201 of the sidewall 200 of described base portion 20, be buckled in the culture bottle bottleneck will give birth to bottle cap 2; The internal diameter of described base portion 20 is arranged to 54 millimeters≤D≤62 millimeter, the height of described funnel type device 21 is arranged to 5 millimeters≤H1≤20 millimeter, the height of described connecting portion 211 is arranged to 1.6 millimeters≤H2≤3 millimeter.In the present embodiment, with described funnel type device 21 and described base portion 20 one-body molded settings, can certainly be arranged to dismountable mode and connect, such as the existing technological means such as can adopt buckle or be threaded, these technological means think that prior art discloses, and does not repeat them here.
In whole grifola frondosus cultivation process, also have one to give birth to the cultivation bottle cap 1 that bottle cap 2 is used in conjunction with the present invention, see also Fig. 4 and Fig. 5, described cultivation bottle cap 1 comprises the main part 10 of being made by light-proof material, filtering bodies 11 and the cover plate 12 of being made by light transmissive material, described main part 10 integral body are hollow cylindrical substantially, comprise sidewall 100 and roof 101, described roof 101 and sidewall 100 are one-body molded and described sidewall 100 is enclosed the spatial separation that forms is upper space 102 and lower space 103 two parts, the penetrating bulging body 104 of the interior formation in bulging space 102 on described one on described roof 101 mediads, described bulging body 104 roughly is hollow cylindrical, the design (such as being designed to through hole) of ventilative and printing opacity is arranged on the top of this bulging body 104, this bulging body 104 is with upper, lower space 102,103 are communicated with, be provided with some air-vents 1021 on the sidewall 1020 in described upper space 102, described air-vent 1021 contiguous roofs 101 arrange, be provided with some air channels 1041 on the sidewall 1040 of described bulging body 104, described air channel is established 1041 and is arranged near the top 1042 of sidewall 1040 of described bulging body 104.Form filtering bodies receiving space 105 between the sidewall 1040 of described bulging body 104 and the sidewall 1020 in upper space 102; The part of described filtering bodies 11 corresponding described bulging bodies 104 hollows out and forms a bulging body receiving space 110, and this filtering bodies 11 is housed in the described filtering bodies receiving space 105; Described cover plate 12 execute the top of covering space 102 on described and described on the upper end of sidewall 1020 in space 102 fasten, and be formed with the airspace (not shown) between the top 1042 of the sidewall 1040 of described bulging body 104 and the cover plate 12.
The internal diameter in described lower space 103 is slightly larger than the diameter of culture bottle (not shown) bottleneck, pass through this time space 103 upper shields on culture bottle in order to cultivate bottle cap 1, be provided with some protrudingly 1032 on sidewall 1030 medial surfaces 1031 in described lower space 103, be buckled in the culture bottle bottleneck will cultivate bottle cap 1; Lower end at the sidewall 1030 in described lower space 103, the sidewall 1030 outside horizontal-extendings in described lower space form support outer rim extension 1033, and the equipment attachment point of cover process is removed in the setting of this support outer rim extension 1033 based on automation inoculation, mycelium stimulation in the planting technique.
Using the present invention to give birth to bottle cap 2 and aforementioned cultivation bottle cap 1, to carry out the flow process of factory culture of grifola frondosus as follows:
(1) bottling, the composts or fertilisers of cultivating of packing in culture bottle is executed to cover to state and is cultivated bottle cap 1;
(2) sterilization moves in the retort and carries out high pressure sterilization;
(3) cooling is moved in the cooling chamber and cools off fast;
(4) inoculation, cooled culture bottle is inoculated by automatic vaccination machine;
(5) cultivate, postvaccinal culture bottle is moved into culturing room to be cultivated, concentration parameter to temperature, humidity and carbonic acid gas is controlled, when the grifola frondosus mycelia is had thorough grasp composts or fertilisers of cultivating substantially, give the scattered light irradiation, cover in 1 bulging body, the 104 formed bulging spaces 1043 until former base restriction is formed on described cultivation, be moved in the mycelium stimulation chamber, remove and cultivate bottle cap 1.
(6) mycelium stimulation encloses the former primitive period of composts or fertilisers of cultivating and to carry out annular mycelium stimulation, executes the described fertility bottle cap 2 of lid; After executing the described fertility bottle cap 2 of lid, originally be limited in the former base in the bulging space, the former base that is limited in giving birth to bottle cap grows in the hole 22.
(7) urge flower bud, the culture bottle behind the mycelium stimulation is moved into urge flower bud chamber relaying supervention to educate, concentration, intensity of illumination and the time parameter of temperature, humidity, carbonic acid gas are controlled, finish and urge flower bud.
(8) fertility is educated finishing the culture bottle immigration fertility chamber relaying supervention of urging flower bud, and concentration, intensity of illumination and the time parameter of temperature, humidity, carbonic acid gas are controlled, and finishes fertility; In growing process, because whole fertility bottle cap all adopts lighttight material to make, so except the former base that cultivation stage forms, other parts can't be subject to light induction, substantially can not produce again new former base, and developing into the restriction that is subject to the funnel type device in the process of fruit body at the former base that cultivation stage concentrate to form, increased substantially the colored shape uniformity of grifola frondosus.
(9) gather, the grifola frondosus of finishing fertility is gathered.
The parameters such as concentration of above-mentioned temperature, humidity and carbonic acid gas can adopt disclosed numerical value in the prior art, and these numerical value are all disclosed by prior art, do not repeat them here.In the flow process of above-mentioned factory culture, cultivating bottle cap 1 role before the incubation step is the same with existing bottle of effect of planting the cultivation bottle cap that uses in the grifola frondosus cultivation process, therefore also can adopt existing bottle to plant the cultivation bottle cap that uses in the grifola frondosus cultivation process in the step before incubation step, the not same-action that cultivation bottle cap 1 is risen is mainly reflected in the cultivation stage of grifola frondosus cultivation.
In order when executing lid fertility bottle cap 2, not damage former base, described former base is grown hole 22 and the 1043 corresponding settings of described bulging space, and the internal diameter that former base is grown hole 22 is arranged to be slightly larger than the internal diameter that equals described bulging space 1043, here " be slightly larger than and equal " is to instigate fertility bottle cap 2 to be executed when being placed on culture bottle, described former base grows hole 22 and can be sleeved on the former base and do not damage former base, such as the internal diameter that described former base grows the hole being arranged to than the wide 1-2 millimeter of bulging space internal diameter.Certainly, can be sleeved on the former base and do not damage former base for convenient former base grows hole 22, also described bulging body 104 can be arranged to the shaped hollow round table of at least part of opening of upper table surface.
Grifola frondosus generally comprises massive texture part and blade-section, and blade-section not only mouthfeel is good, and clean vulnerable to pollution not, so grifola frondosus is take edible blade-section as main, and massive texture partially aqueous amount is larger in addition, and the fresh goods that massive texture is larger is difficult for preserving.Therefore, improving grifola frondosus also is to improve the importance of grifola frondosus quality in the blade differentiation rate of growing stage.When the parameter of fertility bottle cap 2 satisfies following condition: the 54 millimeters≤D of internal diameter of base portion 20≤62 millimeters, 5 millimeters≤the H1 of height of funnel type device 21≤20 millimeters, 1.6 millimeters≤the H2 of height of connecting portion 211≤3 millimeters, the blade differentiation rate of the grifola frondosus that cultivation obtains significantly promotes the blade differentiation rate at (percentage by weight) more than 85%.
Following table is that the culture bottle volume is 800 milliliters, the bottleneck internal diameter is the culture bottle of 52 millimeters (56 millimeters of fertility bottle cap internal diameters), use existing medium and grifola frondosus bacterial classification to cultivate according to cultivation method of the present invention, the blade differentiation rate (percentage by weight) corresponding to parameter of fertility bottle cap 2.
 
Figure DEST_PATH_IMAGE002
Above description is embodiments of the invention only, forgives and can understand, and under the prerequisite that does not depart from the present invention's design, all should be included within the technical conceive of the present invention simple modification of the present invention and replacement.

Claims (3)

1. a grifola frondosus cultivation is with giving birth to bottle cap, it is characterized in that: described fertility bottle cap is made by light-proof material, comprise base portion and be arranged at the funnel type device of described base portion top, described fertility bottle cap adds by described base portion and is placed on the culture bottle mouth, described funnel type device grows the hole with base portion by former base and is communicated with, described funnel type device comprises mushroom flower bud former and connects the connecting portion of mushroom flower bud former and base portion, the internal diameter of described base portion satisfies 54 millimeters≤D≤62 millimeters, the height of described funnel type device satisfies 5 millimeters≤H1≤20 millimeters, and the height of described connecting portion satisfies 1.6 millimeters≤H2≤3 millimeters.
2. grifola frondosus cultivation as claimed in claim 1 is with giving birth to bottle cap, and it is characterized in that: described funnel type device and described base portion are one-body molded.
3. grifola frondosus cultivation as claimed in claim 1 is with giving birth to bottle cap, and it is characterized in that: described funnel type device and base portion link together removably.
CN2011102988572A 2011-09-28 2011-09-28 Growth bottle lid for grifola frondosa cultivation Pending CN103004460A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2011102988572A CN103004460A (en) 2011-09-28 2011-09-28 Growth bottle lid for grifola frondosa cultivation

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Application Number Priority Date Filing Date Title
CN2011102988572A CN103004460A (en) 2011-09-28 2011-09-28 Growth bottle lid for grifola frondosa cultivation

Publications (1)

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CN103004460A true CN103004460A (en) 2013-04-03

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002218844A (en) * 2000-11-21 2002-08-06 Hokuto Corp Method for culturing grifola frondosa and cap for bottle for culturing grifola fondosa used in the same method
JP2003339233A (en) * 2002-05-29 2003-12-02 Takeuchi Seisakusho:Kk Cap for mushroom cultivation bottle
JP2006223264A (en) * 2005-02-21 2006-08-31 Misuzu Life:Kk Mushroom cultivation method and cap used therefor
JP4747333B2 (en) * 2000-11-21 2011-08-17 ホクト株式会社 Maitake cultivation bottle set
CN202232253U (en) * 2011-09-28 2012-05-30 上海丰科生物科技股份有限公司 Growing bottle cap for cultivation of mitake mushroom

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002218844A (en) * 2000-11-21 2002-08-06 Hokuto Corp Method for culturing grifola frondosa and cap for bottle for culturing grifola fondosa used in the same method
JP4747333B2 (en) * 2000-11-21 2011-08-17 ホクト株式会社 Maitake cultivation bottle set
JP2003339233A (en) * 2002-05-29 2003-12-02 Takeuchi Seisakusho:Kk Cap for mushroom cultivation bottle
JP2006223264A (en) * 2005-02-21 2006-08-31 Misuzu Life:Kk Mushroom cultivation method and cap used therefor
CN202232253U (en) * 2011-09-28 2012-05-30 上海丰科生物科技股份有限公司 Growing bottle cap for cultivation of mitake mushroom

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Application publication date: 20130403