CN102921481A - Microfluid array device - Google Patents
Microfluid array device Download PDFInfo
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- CN102921481A CN102921481A CN2012104253861A CN201210425386A CN102921481A CN 102921481 A CN102921481 A CN 102921481A CN 2012104253861 A CN2012104253861 A CN 2012104253861A CN 201210425386 A CN201210425386 A CN 201210425386A CN 102921481 A CN102921481 A CN 102921481A
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Abstract
The invention relates to a microfluid structure, in particular relates to a microfluid array device, and solves the problems that the conventional microfluid structure for biological detection is not beneficial to extraction of micro detection signals and can easily damage detected cells. The microfluid array device comprises a microfluid array layer, a coverage layer bonded on the upper surface of the microfluid array layer, and a substrate layer attached and fixed at the lower surface of the microfluid array layer, wherein two columns of microfluid structure units, and two longitudinal microfluid straight channels parallel with each other are respectively formed on the upper surface of the microfluid array layer; one column of microfluid structure units comprises the same number of microfluid structure units (which are linearly arranged at equal intervals) as that of the other column of microfluid structure units; and each microfluid structure unit comprises a micro reaction cavity and a transverse microfluid straight channel communicated with the micro reaction cavity. The microfluid array device is suitable for the fields of cell detection, cell cultivation, medicine screening, medicine transportation and the like.
Description
Technical field
The present invention relates to microfluidic structures, specifically a kind of microfluidic arrays device.
Background technology
Micro-fluidic technologies is the study hotspot in current micro electronmechanical field, and it mainly comprises cell detection, cell cultivation, drug screening, medicament transport etc., usually is widely used in the fields such as bioengineering, environmental monitoring, food engineering, clinical medicine.Biology sensor is a class is studied various biological phenomenas at molecular level important tool, and it has the advantages such as specificity is high, sensitivity is high, but also there are the shortcomings such as weak output signals, poor anti jamming capability simultaneously in it.As integrated, the microminiaturized a kind of requisite key technology of biology sensor, micro-fluidic technologies can strengthen the output signal of biology sensor, the antijamming capability of raising biology sensor.Therefore, the biology sensor (microfluidic structures that namely is used for biological detection) based on the micro-fluidic technologies exploitation just is widely applied.Under the prior art condition, the microfluidic structures that is used for biological detection mainly is to send into reaction microchamber by the liquid that miniflow straight flute road directly will contain detected cell to detect.The shortcoming that this kind is used for the microfluidic structures of biological detection is: one, the liquid that contains detected cell is entering in the process of reaction microchamber by miniflow straight flute road, can in reaction microchamber, form convection current and turbulent flow, convection current and turbulent flow can be brought the vibrations noise, and the vibrations noise tends to cover detected small detection signal, is unfavorable for the extraction of small detection signal.They are two years old, the liquid that contains detected cell is entering in the process of reaction microchamber by miniflow straight flute road, it is excessive flow velocity to appear, and flow velocity is crossed conference to the detected cell formation shearing force in the liquid, in case but shearing force exceeds the tolerance range of detected cell, will damage detected cell, cause detected cell to lose activity, and then cause detected cell to lose Clinical significance of detecting.Based on this, be necessary to invent a kind of brand-new microfluidic structures that is used for biological detection, be unfavorable for the extraction of small detection signal and the problem of the detected cell of easy infringement to solve the existing microfluidic structures that is used for biological detection.
Summary of the invention
The present invention is unfavorable for the extraction of small detection signal and the problem of easily damaging detected cell in order to solve the existing microfluidic structures that is used for biological detection, and a kind of microfluidic arrays device is provided.
The present invention adopts following technical scheme to realize: a kind of microfluidic arrays device comprises the microfluidic arrays layer, is bonded to the cover layer of upper surface of microfluidic arrays layer and the substrate layer that attaches the lower surface that is fixed in the microfluidic arrays layer; Wherein, the upper surface of microfluidic arrays layer offers respectively two row microfluidic structures unit and two vertical miniflow straight flute roads that are parallel to each other; Two row microfluidic structures unit respectively comprise the microfluidic structures unit that the equidistant line of same number is arranged, and the orientation of every row microfluidic structures unit is all parallel with vertical miniflow straight flute road; Each microfluidic structures unit includes a reaction microchamber and a horizontal miniflow straight flute road that is communicated with reaction microchamber; Wherein the horizontal miniflow straight flute of each bar road in the row microfluidic structures unit all with vertical miniflow straight flute road vertical connection wherein; The horizontal miniflow straight flute of each bar road in another row microfluidic structures unit all with another vertical miniflow straight flute road vertical connection; The two ends in every vertical miniflow straight flute road all are communicated with an input and output micropore, and four input and output micropores all are opened in the upper surface of microfluidic arrays layer; Tectal upper surface offers respectively four dredging holes, and four dredging holes respectively with four input and output micropore positions over against.The size of described vertical miniflow straight flute road, reaction microchamber, horizontal miniflow straight flute road, input and output micropore, dredging hole all is decided according to the actual requirements.
During work, with two vertical miniflow straight flute roads separately two dredging holes of an end be connected with external peristaltic pump by capillary, and with two vertical miniflow straight flute roads separately two dredging holes of the other end be communicated with outside waste liquid pool.The specific works process is as follows: at first, the liquid that external peristaltic pump will contain detected cell injects separately two dredging holes of an end of two vertical miniflow straight flute roads simultaneously.Then, the liquid that contains detected cell two the vertical miniflow straight flute roads of flowing through simultaneously, and diffuse to simultaneously in the two row reaction microchambers by the horizontal miniflow straight flute of two row road.At last, the liquid that contains detected cell simultaneously by two vertical miniflow straight flute roads separately two dredging holes of the other end be back to outside waste liquid pool, realized thus containing the dynamic circulation of the liquid of detected cell.In this process, by outside fluorescence microscope, can see through cover layer the liquid that contains detected cell in the two row reaction microchambers is detected, to realize detecting the purpose of cell.Based on said process, compare with the existing microfluidic structures that is used for biological detection, a kind of microfluidic arrays device of the present invention has the following advantages: one, a kind of microfluidic arrays device of the present invention adopts the structural design of two row reaction microchambers, wherein a row reaction microchamber can be used for providing the main detection signal of cell, another row reaction microchamber can be used for providing the contrast detection signal of cell, carry out calculus of differences by the detection signal that two row reaction microchambers are provided, can be with various non-artificial noises (the vibrations noise that brings such as convection current and turbulent flow, the impact that non-specific adsorption is exported the sensing unit signal etc.) get rid of, realize thus the extraction of small detection signal, and then guarantee the accuracy of testing result.They are two years old, a kind of microfluidic arrays device of the present invention adopts the microfluidic structures unit that is comprised of reaction microchamber and horizontal miniflow straight flute road, realized that the liquid that will contain detected cell slowly diffuses in the reaction microchamber, effectively avoided thus flow rate of liquid excessive, effectively reduce liquid to the shearing force of detected cell, thereby avoided detected cell to be compromised, guaranteed the normal detection of detected cell.In sum, a kind of microfluidic arrays device of the present invention efficiently solves the existing microfluidic structures that is used for biological detection and is unfavorable for the extraction of small detection signal and the problem of easily damaging detected cell based on brand-new structure.By following experiment, can further verify the above-mentioned advantage of a kind of microfluidic arrays device of the present invention: as shown in Figure 4, by a microfluidic structures unit is carried out velocity field emulation, can draw: the diffusion velocity of liquid in reaction microchamber reduced 10 than its speed in vertical miniflow straight flute road
4The order of magnitude, and VELOCITY DISTRIBUTION is even, thereby the microfluidic structures unit in a kind of microfluidic arrays device of the present invention in fact built a stable again microenvironment of relative closure for detected cell, for its division cycle is cultivated, observed to cell or the pathology state provides strong guarantee.
The present invention efficiently solves the existing microfluidic structures that is used for biological detection and is unfavorable for the extraction of small detection signal and the problem of easily damaging detected cell, it has the series of advantages such as simple, easy to operate, the integrated degree of making is high, microminiaturized, not only can cultivate and detect different cells, and can be integrated in the different biochip integrated systems, be applicable to the fields such as cell detection, cell cultivation, drug screening, medicament transport.
Description of drawings
Fig. 1 is structural representation of the present invention.
Fig. 2 is the side view of Fig. 1.
Fig. 3 is the structural representation of microfluidic arrays layer of the present invention.
Fig. 4 is the velocity field analogous diagram of a microfluidic structures unit of the present invention.
Among the figure: 1-microfluidic arrays layer, 2-cover layer, 3-substrate layer, the vertical miniflow straight flute of 4-road, 5-reaction microchamber, the horizontal miniflow straight flute of 6-road, 7-input and output micropore, 8-dredging hole.
The specific embodiment
A kind of microfluidic arrays device comprises microfluidic arrays layer 1, is bonded to the cover layer 2 of upper surface of microfluidic arrays layer 1 and the substrate layer 3 that attaches the lower surface be fixed in microfluidic arrays layer 1; Wherein, the upper surface of microfluidic arrays layer 1 offers respectively two row microfluidic structures unit and two vertical miniflow straight flute roads 4 that are parallel to each other; Two row microfluidic structures unit respectively comprise the microfluidic structures unit that the equidistant line of same number is arranged, and the orientation of every row microfluidic structures unit is all parallel with vertical miniflow straight flute road 4; Each microfluidic structures unit includes a reaction microchamber 5 and a horizontal miniflow straight flute road 6 that is communicated with reaction microchamber 5; Wherein the horizontal miniflow straight flute of each bar road 6 in the row microfluidic structures unit all with vertical miniflow straight flute road 4 vertical connections wherein; The horizontal miniflow straight flute of each bar road 6 in another row microfluidic structures unit all with another vertical miniflow straight flute road 4 vertical connections; The two ends in every vertical miniflow straight flute road 4 all are communicated with an input and output micropore 7, and four input and output micropores 7 all are opened in the upper surface of microfluidic arrays layer 1; The upper surface of cover layer 2 offers respectively four dredging holes 8, and four dredging holes 8 respectively with four input and output micropore 7 positions over against;
Article two, identical length in vertical miniflow straight flute road 4 etc.; Article two, the width in vertical miniflow straight flute road 4 all equates; Article two, the degree of depth in vertical miniflow straight flute road 4 all equates;
Each reaction microchamber 5 is circular reaction microchamber; The diameter of each reaction microchamber 5 all equates; The degree of depth of each reaction microchamber 5 all equates;
The identical length in the horizontal miniflow straight flute of each bar road 6 etc.; The width in the horizontal miniflow straight flute of each bar road 6 all equates; The degree of depth in the horizontal miniflow straight flute of each bar road 6 all equates;
Four input and output micropores 7 are circular input and output micropore; The diameter of four input and output micropores 7 all equates; The degree of depth of four input and output micropores 7 all equates;
Four dredging holes 8 are circular dredging hole; The diameter of four dredging holes 8 all equates; The degree of depth of four dredging holes 8 all equates;
During implementation, microfluidic arrays layer and cover layer all adopt dimethyl silicone polymer (PDMS) to make.Wherein, cover layer adopts transparent dimethyl silicone polymer (PDMS) to make, and is convenient to see through cover layer by microscope cell is detected.Substrate layer adopts glass to make.Article two, the width in vertical miniflow straight flute road is 125 μ m.Article two, the degree of depth in vertical miniflow straight flute road is 60 μ m.The diameter of each reaction microchamber is 600 μ m.The degree of depth of each reaction microchamber is 60 μ m.The length in the horizontal miniflow straight flute of each bar road is 80 μ m.The width in the horizontal miniflow straight flute of each bar road is 80 μ m.The degree of depth in the horizontal miniflow straight flute of each bar road is 60 μ m.The diameter of four input and output micropores is 150 μ m.
Claims (6)
1. microfluidic arrays device is characterized in that: comprise microfluidic arrays layer (1), be bonded to the cover layer (2) of upper surface of microfluidic arrays layer (1) and the substrate layer (3) that attaches the lower surface that is fixed in microfluidic arrays layer (1); Wherein, the upper surface of microfluidic arrays layer (1) offers respectively two row microfluidic structures unit and two vertical miniflow straight flute roads (4) that are parallel to each other; Two row microfluidic structures unit respectively comprise the microfluidic structures unit that the equidistant line of same number is arranged, and the orientation of every row microfluidic structures unit is all parallel with vertical miniflow straight flute road (4); Each microfluidic structures unit includes a reaction microchamber (5) and a horizontal miniflow straight flute road (6) that is communicated with reaction microchamber (5); Wherein the horizontal miniflow straight flute of each bar road (6) in the row microfluidic structures unit all with vertical miniflow straight flute road (4) vertical connection wherein; The horizontal miniflow straight flute of each bar road (6) in another row microfluidic structures unit all with another vertical miniflow straight flute road (4) vertical connection; The two ends in every vertical miniflow straight flute road (4) all are communicated with an input and output micropore (7), and four input and output micropores (7) all are opened in the upper surface of microfluidic arrays layer (1); The upper surface of cover layer (2) offers respectively four dredging holes (8), and four dredging holes (8) respectively with four input and output micropores (7) position over against.
2. a kind of microfluidic arrays device according to claim 1 is characterized in that: the identical length in two vertical miniflow straight flute roads (4) etc.; Article two, the width in vertical miniflow straight flute road (4) all equates; Article two, the degree of depth in vertical miniflow straight flute road (4) all equates.
3. a kind of microfluidic arrays device according to claim 1, it is characterized in that: each reaction microchamber (5) is circular reaction microchamber; The diameter of each reaction microchamber (5) all equates; The degree of depth of each reaction microchamber (5) all equates.
4. a kind of microfluidic arrays device according to claim 1 is characterized in that: the identical length in the horizontal miniflow straight flute of each bar road (6) etc.; The width in the horizontal miniflow straight flute of each bar road (6) all equates; The degree of depth in the horizontal miniflow straight flute of each bar road (6) all equates.
5. a kind of microfluidic arrays device according to claim 1, it is characterized in that: four input and output micropores (7) are circular input and output micropore; The diameter of four input and output micropores (7) all equates; The degree of depth of four input and output micropores (7) all equates.
6. a kind of microfluidic arrays device according to claim 1, it is characterized in that: four dredging holes (8) are circular dredging hole; The diameter of four dredging holes (8) all equates; The degree of depth of four dredging holes (8) all equates.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599722A (en) * | 2013-11-22 | 2014-02-26 | 上海交通大学 | Bionics microfluidic mixer |
| CN104777161A (en) * | 2015-03-27 | 2015-07-15 | 北京大学 | Immobilized microcapsule detection array, and processing method and application thereof |
| CN106378217A (en) * | 2016-12-06 | 2017-02-08 | 太原理工大学 | Micro-fluidic chip capable of producing two kinds of droplets including oil-water-oil droplets and water-oil-water droplets |
| CN111443080A (en) * | 2019-01-17 | 2020-07-24 | 大韩民国(国立水产科学院) | Intelligent water quality measuring system for aquaculture |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2565022Y (en) * | 2002-08-23 | 2003-08-06 | 上海博昇微晶科技有限公司 | Microfluid chip with three-layer structure |
| EP1415788A1 (en) * | 2002-10-31 | 2004-05-06 | Agilent Technologies, Inc. | Integrated microfluidic array device |
| CN1996014A (en) * | 2006-12-12 | 2007-07-11 | 中国人民解放军第二军医大学 | Array micro-fluidic chip device for use in drug metabolism screening |
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2012
- 2012-10-31 CN CN2012104253861A patent/CN102921481A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2565022Y (en) * | 2002-08-23 | 2003-08-06 | 上海博昇微晶科技有限公司 | Microfluid chip with three-layer structure |
| EP1415788A1 (en) * | 2002-10-31 | 2004-05-06 | Agilent Technologies, Inc. | Integrated microfluidic array device |
| CN1996014A (en) * | 2006-12-12 | 2007-07-11 | 中国人民解放军第二军医大学 | Array micro-fluidic chip device for use in drug metabolism screening |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599722A (en) * | 2013-11-22 | 2014-02-26 | 上海交通大学 | Bionics microfluidic mixer |
| CN104777161A (en) * | 2015-03-27 | 2015-07-15 | 北京大学 | Immobilized microcapsule detection array, and processing method and application thereof |
| CN106378217A (en) * | 2016-12-06 | 2017-02-08 | 太原理工大学 | Micro-fluidic chip capable of producing two kinds of droplets including oil-water-oil droplets and water-oil-water droplets |
| CN111443080A (en) * | 2019-01-17 | 2020-07-24 | 大韩民国(国立水产科学院) | Intelligent water quality measuring system for aquaculture |
| CN111443080B (en) * | 2019-01-17 | 2023-06-06 | 大韩民国(国立水产科学院) | Intelligent water quality measurement system for aquaculture |
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Application publication date: 20130213 |