CN201686701U

CN201686701U - Polymerase chain reaction (PCR) capillary column gene chip

Info

Publication number: CN201686701U
Application number: CN2009200346396U
Authority: CN
Inventors: 郭晏海; 颜真; 赵锦荣; 汪钦; 梁平; 包晗; 雷小英; 张菊; 刘永兰; 随文君
Original assignee: Fourth Military Medical University FMMU
Current assignee: Fourth Military Medical University FMMU
Priority date: 2009-09-18
Filing date: 2009-09-18
Publication date: 2010-12-29
Anticipated expiration: 2019-09-18

Abstract

The utility model relates to a polymerase chain reaction (PCR) capillary column gene chip which hybridizes the nucleic acid PCR and a probe. The existing conventional PCR capillary tube is only used as a reaction vessel for nucleic acid amplification, if further gene chip analysis is carried out for PCR amplified product, the PCR product is required to be absorbed out and added on the gene chip for chip and probe hybridization analysis, and the operation is troublesome. The PCR capillary column gene chip consists of a PCR capillary reaction tube and a capillary column gene chip, wherein the capillary column gene chip is fixed at the center of the PCR capillary tube; and a nucleic acid probe is marked on the capillary column gene chip. During detection, the prepared PCR reaction solution is added in the PCR capillary column gene chip, then the PCR capillary column gene chip is placed in a special nucleic acid amplification and hybridization detector for PCR amplification and probe hybridization detection, the integration of the nucleic acid amplification and chip and probe hybridization analysis is realized, and the gene chip hybridization analysis is simpler and faster.

Description

Polymerase chain reaction,PCR capillary column gene chip

Affiliated technical field

The utility model relates to a kind of nucleic acid amplification and chip probe is hybridized incorporate polymerase chain reaction,PCR (PCR) capillary column gene chip.

Background technology

Existing conventional PCR kapillary only is the reaction vessel as nucleic acid amplification, if will do further gene chip analysis to pcr amplification product, then the sucking-off of PCR reaction product will be added to and carry out the chip probe hybridization analysis on the gene chip, this experimental implementation is comparatively loaded down with trivial details.And the incorporate device of PCR and gene chip is micro-fluid chip or micro-fluidic chip with the preparation of MEMS technology mostly, such chip can reach sample preparation, pcr amplification, chip detection in one, but such chip has many problems to wait to solve, for example complex manufacturing, production cost height, poor practicability.

Summary of the invention

In order to overcome the deficiency of the integrated gene chip complex manufacturing of existing nucleic acid amplification and chip probe hybridization analysis, production cost height, poor practicability, the utility model provides a kind of easier production, practical, amplification and hybridizes incorporate PCR capillary column gene chip.

The technical scheme that its technical problem that solves the utility model adopts is: a kind of PCR capillary column gene chip is to be made of PCR kapillary reaction tubes and capillary column gene chip.It is characterized in that: the capillary column 4 of capillary column gene chip is to be fixed in the kapillary 3 by fixing empennage 5.

Described PCR kapillary reaction tubes is made up of reaction tubes lid 1, reaction tubes cavity 2 and kapillary 3.

Described capillary column gene chip is by capillary column 4, fixedly empennage 5 constitutes.Wherein, the surface markers of capillary column 4 has nucleic acid probe molecules 6.

Above-mentioned reaction tubes lid 1 and reaction tubes cavity 2 adopt polypropylene material.

Above-mentioned kapillary 3 adopts glass material.

Above-mentioned capillary column 4 adopts organic optical materials.

Said fixing empennage 5 adopts polypropylene material.

The beneficial effects of the utility model are:

1, be convenient to produce preparation: this utility model directly places the capillary column chip kapillary 3 of PCR kapillary reaction tubes aborning, and production operation is simple.

2, nucleic acid amplification and chip hybridization are easy to operate: the utility model adds the design of capillary column chip on conventional PCR basis capillaceous, nucleic acid amplification and chip hybridization is detected be integrated.Compared with prior art, better practicality and compatibility are arranged.

Description of drawings

Below in conjunction with drawings and Examples the utility model is further specified.

Fig. 1 is an outside drawing of the present utility model;

Fig. 2 is the longitudinal diagram of Fig. 1;

Fig. 3 is a capillary column chip longitudinal diagram;

Fig. 4 is the figure that makes a general survey of of capillary column chip

1. reaction tubess lid among the figure, 2. reaction tubes cavity, 3. kapillary, 4. capillary column, 5. fixing empennage, 6, nucleic acid probe molecules.

Embodiment

Referring to Fig. 1, Fig. 2, Fig. 3, Fig. 4, the utility model---PCR capillary column gene chip is to be made of PCR kapillary reaction tubes and capillary column gene chip.Described PCR kapillary reaction chamber is made up of reaction tubes lid (1), reaction tubes cavity (2) and kapillary (3), kapillary wherein (3) is fixed in the low side of reaction tubes cavity (2), and pcr amplification reaction liquid mixes to be incorporated in reaction tubes cavity (2) and carries out the PCR reaction in the kapillary (3).The afterbody of the capillary column of capillary column gene chip (4) is connected with fixedly empennage (5), capillary column (4) is fixed in the kapillary (3) by fixing empennage (5), and the capillary column surface is fixed with different nucleic acid probe molecules (6) respectively by the mode of getting ready or ruling.When nucleic acid amplification and hybridization detection, open the reaction tubes lid (1) of PCR capillary column gene chip, the pcr amplification reaction liquid for preparing is added in the reaction tubes cavity (2), build reaction tubes lid (1), by centrifugal with the centrifugal injection kapillary of above-mentioned reaction solution (3), then PCR capillary column gene chip is put into special-purpose nucleic acid amplification and hybridization detecting instrument, carried out pcr amplification and hybridization and detect, thereby reach nucleic acid amplification and the integrated detection of hybridization.

Claims

1. polymerase chain reaction,PCR capillary column gene chip comprises that PCR kapillary reaction tubes and capillary column gene chip constitute, and is characterized in that: the capillary column of capillary column gene chip (4) is to be fixed in the kapillary (3) by fixing empennage (5).

2. polymerase chain reaction,PCR capillary column gene chip according to claim 1 is characterized in that: the capillary column gene chip be by capillary column (4) and fixedly empennage (5) constitute.

3. according to the polymerase chain reaction,PCR capillary column gene chip of claim 1 or 2, it is characterized in that: described capillary column (4) adopts organic optical materials.

4. according to the polymerase chain reaction,PCR capillary column gene chip of claim 1 or 2, it is characterized in that: described fixedly empennage (5) adopts polypropylene material.