CN102525569B - Array type microdialysis probe - Google Patents
Array type microdialysis probe Download PDFInfo
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- CN102525569B CN102525569B CN 201110444617 CN201110444617A CN102525569B CN 102525569 B CN102525569 B CN 102525569B CN 201110444617 CN201110444617 CN 201110444617 CN 201110444617 A CN201110444617 A CN 201110444617A CN 102525569 B CN102525569 B CN 102525569B
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Abstract
The invention discloses an array type microdialysis probe. The array type microdialysis probe comprises a liquid inlet protecting tube, a liquid inlet sealing column, a liquid outlet sealing column, a liquid outlet protecting tube, sealing epoxy resin, a base seat and a plurality of microdialysis probes, wherein the microdialysis probe comprises a high molecular hollow fiber membrane, a stent catheter, a liquid inlet capillary and a liquid outlet capillary. The high molecular hollow fiber membrane and the stent catheter are fixedly connected by the epoxy resin. The high molecular hollow fiber membrane is communicated with the inner cavity of the stent catheter. The base seat is provided with a plurality of through holes. A microdialysis probe is fixed in each through hole of the base seat. The liquid inlet protecting tube is sealed by the liquid inlet sealing column. The liquid outlet protecting tube is sealed by the liquid outlet sealing column. The microdialysis probes are connected in series, in parallel or grouping manner. The probe array disclosed by the invention can be used in a series or parallel manner, so that the effective dialysis area is enlarged and the recovery of organism living body fluid sampling is improved.
Description
Technical field
The present invention relates to a kind of zoopery equipment, relate in particular to a kind of array micro-dialysis device.
Background technology
Micro-dialysis device is can pass through semipermeable membrane with micromolecule and water, along Concentraton gradient spread and macromolecular substances can not to see through semipermeable membrane be a kind of chemical substance sampling apparatus that basis be designed.In the tubulose dialyzer by minor diameter is implanted animal body behind specific region, with artificial dialysis solution continous perfusion, the small-molecule substance in the outer liquid of histiocyte forms Concentraton gradient in the semipermeable membrane both sides, and diffuse into dialysis solution along Concentraton gradient, and by dialysis solution, taken out of external, by the analysis to content of material in dialysis solution, can calculate the concentration level of this material in the outer liquid of in-vivo tissue.This technology is applied to monitor the research of the interior chemical micro-environment variation of body and drug mechanism.
The key parameter of micro-dialysis device is the response rate of material, the ratio of material concentration in material concentration and probe environmental liquids in the dialysis solution reclaimed.The relating to parameters such as this parameter and target substance molecular size range, dialysis membrane material and dialyzer length, ambient temperature, perfusion flow velocity.For predetermined substance and certain ambient temperature, in order to obtain the higher probe response rate, generally can adopt the method that increases dialyzer length and reduce the perfusion flow velocity.For little laboratory animal as rat, mice etc., physical dimension is less in vertical direction as hypothalamus etc. for its part all brain structures, the effective length of the probe film that can hold is limited, therefore probe is lower to the response rate of endogenous neurotransmitter or ectogenic pharmacological active substance, affects the detectability that dialysis solution is analyzed.As passed through to reduce rate of flooding, though can improve the response rate of probe, but can reduce temporal resolution, unfavorable to dynamic analysis.Therefore existing microdialysis probe pedestal has certain volume, is limited to the laboratory animal head volume limited, is merely able to simultaneously respectively to implant microdialysis probe 1～3 spacing in far away different brain cores group, carries out the synchronous microdialysis of a plurality of core group.
Summary of the invention
The objective of the invention is for the deficiencies in the prior art, a kind of array type microdialysis probe is provided.
The objective of the invention is to be achieved through the following technical solutions: a kind of array type microdialysis probe, it comprises: enter the liquid protecting tube, enter the liquid sealing post, fluid sealing column, fluid protecting tube, sealing epoxy resin, pedestal and several microdialysis probes; Wherein, microdialysis probe comprises: hollow fiber polymer membrane, scaffold tube, enter liquid capillary tube and fluid capillary tube, hollow fiber polymer membrane fixedly is connected by epoxy resin with scaffold tube, and the inner chamber of hollow fiber polymer membrane and scaffold tube communicates; Pedestal has some through holes, fixes a microdialysis probe in each through hole of pedestal, enters the liquid protecting tube by entering the liquid sealing column sealing, and the fluid protecting tube, by the sealing of fluid sealing column, forms the annexation of series, parallel or grouping between each microdialysis probe.
Further, the annexation of described series connection is specially: also comprise some for connecting the connection protecting tube of microdialysis probe, enter liquid protecting tube and fluid protecting tube and be one, the liquid capillary tube that enters of first microdialysis probe passes into the liquid protecting tube, after the fluid capillary tube of first microdialysis probe connects protecting tube by first, with the liquid capillary tube that enters of second microdialysis probe, communicate, after the fluid capillary tube of second microdialysis probe connects protecting tube by second, with the liquid capillary tube that enters of the 3rd microdialysis probe, communicate, the like, the fluid capillary tube of last microdialysis probe passes the fluid protecting tube, enter the liquid protecting tube, microdialysis probe, the junction connected between protecting tube and fluid protecting tube is connected with epoxy resin by connecting tube.Entering liquid protecting tube, connection protecting tube and fluid protecting tube is sealed and is fixed on pedestal by sealing epoxy resin.
Further, the annexation of described parallel connection is specially: enter liquid protecting tube and fluid protecting tube and be one, the liquid capillary tube that enters of each microdialysis probe passes respectively into the liquid protecting tube, and the fluid capillary tube of each microdialysis probe passes same fluid protecting tube; The junction entered between liquid protecting tube, microdialysis probe and fluid protecting tube is connected with epoxy resin by connecting tube; Enter the liquid protecting tube and the fluid protecting tube is sealed and is fixed on pedestal by sealing epoxy resin.
Further; the annexation of described grouping is specially: the number that enters liquid protecting tube and fluid protecting tube is N; microdialysis probe is divided into the N group; each organizes the length difference of microdialysis probe; the length of microdialysis probe is identical on the same group; the number of every group of microdialysis probe is 1 or is greater than 1, and when the number of every group of microdialysis probe, when being greater than 1, each microdialysis probe forms the annexation of serial or parallel connection.
The invention has the beneficial effects as follows, the present invention adapts to the shape of cerebral tissue structure and the restriction in space, increases effective dialysis area, improves the response rate of material sampling in small brain structure.
The accompanying drawing explanation
Fig. 1 is equidistant, the isometric tandem array type microdialysis probe of the embodiment of the present invention 1 structural representation;
Fig. 2 is the not isometric tandem array type microdialysis probe structural representation of the embodiment of the present invention 2 grouping;
Fig. 3 is equidistantly not isometric parallel array type microdialysis probe structural representation of the embodiment of the present invention 3;
In figure, microdialysis probe 1, hollow fiber polymer membrane 2, epoxy resin 3, scaffold tube 4, enter liquid capillary tube 5, fluid capillary tube 6, through hole 7, epoxy resin 8 for connecting tube, enter liquid protecting tube 9, enter liquid sealing post 10, connect protecting tube 11, fluid sealing column 12, fluid protecting tube 13, sealing epoxy resin 14, pedestal 15.
The specific embodiment
Below describe according to drawings and embodiments the present invention in detail, it is more obvious that purpose of the present invention and effect will become.
Embodiment 1
As shown in Figure 1, the array type microdialysis probe of this embodiment comprises: enter liquid protecting tube 9, enter liquid sealing post 10, connect protecting tube 11, fluid sealing column 12, fluid protecting tube 13, sealing epoxy resin 14, pedestal 15 and several microdialysis probes 1.Wherein, microdialysis probe 1 comprises: hollow fiber polymer membrane 2, scaffold tube 4, enter liquid capillary tube 5 and fluid capillary tube 6, hollow fiber polymer membrane 2 is fixedly connected with by epoxy resin 3 with scaffold tube 4, the inner chamber of hollow fiber polymer membrane 2 and scaffold tube 4 communicates, shown in pedestal 15 there are some through holes, microdialysis probe 1 is separately fixed in the through hole of pedestal 15, in this embodiment, array type microdialysis probe is that tandem connects, that is: the liquid capillary tube 5 that enters of first microdialysis probe 1 passes into liquid protecting tube 9, the fluid capillary tube 6 of first microdialysis probe 1 is by after connecting protecting tube 11, with the liquid capillary tube 5 that enters of second microdialysis probe 1, communicate, the fluid capillary tube 6 of second microdialysis probe 1 is by after connecting protecting tube 11, with the liquid capillary tube 5 that enters of the 3rd microdialysis probe 1, communicate, the like, the fluid capillary tube 6 of last microdialysis probe 1 passes fluid protecting tube 13, enter liquid protecting tube 9 by entering 10 sealings of liquid sealing post, fluid protecting tube 13 is by 12 sealings of fluid sealing column, enter liquid protecting tube 9, microdialysis probe 1, the junction connected between protecting tube 11 and fluid protecting tube 13 is connected with epoxy resin 8 by connecting tube.Entering liquid protecting tube 9, connection protecting tube 11 and fluid protecting tube 13 seals and is fixed on pedestal 15 by sealing epoxy resin 14.
In this embodiment, all microdialysis probes 1 are isometric, series connection each other, and the film that has increased effective dialysis is long, has improved the dialysis response rate.But microdialysis probe 1 can be not isometric yet, thereby the cerebral tissue that can adapt to simultaneously different depth carries out microdialysis simultaneously.
In Fig. 1,4 grades microdialysis probe 1 of looking is arranged, but, in actual production process, can freely adjust as required the number of microdialysis probe 1.
Embodiment 2
As shown in Figure 2; with the difference of embodiment 1, be; microdialysis probe 1 is divided into several groups; every group of microdialysis probe 1 is isometric; and as shown in Figure 1; enter liquid protecting tube 9, a fluid protecting tube 13 and some protecting tubes 11 that connects and form the relation that is connected in series with one respectively, not the length difference of the microdialysis probe 1 between on the same group.
In this embodiment, the length difference of the microdialysis probe 1 between on the same group not, and do not form independently series relationship between on the same group therefore, can be respectively carried out microdialysis to the cerebral tissue of different depth simultaneously, improves dialytic efficiency.
In Fig. 2, microdialysis probe 1 is divided into different two groups of length, every group of two isometric microdialysis probe 1 and one enter liquid protecting tube 9, fluid protecting tube 13, one connect protecting tube 11 and form independently series relationship.But, in actual production process, can freely adjust as required the group number of microdialysis probe 1, and the number of the microdialysis probe 1 of every group.
As shown in Figure 3, with the difference of embodiment 1, be, do not connect protecting tube 11, the liquid capillary tube 5 that enters of each microdialysis probe 1 passes respectively the same liquid protecting tube 9 that enters, and fluid capillary tube 6 passes same fluid protecting tube 13, forms the relation that is connected in parallel.
In this embodiment, comprise that one enters liquid protecting tube 9, fluid protecting tube 13 and some microdialysis probes 1, each some microdialysis probes 1 form annexation in parallel, can to the cerebral tissue of multiple spot, be dialysed simultaneously.
In Fig. 3,4 microdialysis probes 1 are arranged, but, in actual production process, can freely adjust as required the number of microdialysis probe 1; The length difference of each microdialysis probe 1, adapt to the vicissitudinous cerebral tissue microdialysis of the degree of depth, and in actual production process, the length of each microdialysis probe 1 also can be identical, carries out the cerebral tissue microdialysis of the same degree of depth.
Above-described embodiment is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change that the present invention is made, all fall into protection scope of the present invention.
Claims (4)
1. an array type microdialysis probe, it is characterized in that, it comprises: enter liquid protecting tube (9), enter liquid sealing post (10), fluid sealing column (12), fluid protecting tube (13), sealing epoxy resin (14), pedestal (15) and several microdialysis probes (1); Wherein, microdialysis probe (1) comprising: hollow fiber polymer membrane (2), scaffold tube (4), enter liquid capillary tube (5) and fluid capillary tube (6), hollow fiber polymer membrane (2) is fixedly connected with by epoxy resin (3) with scaffold tube (4), and the inner chamber of hollow fiber polymer membrane (2) and scaffold tube (4) communicates; Pedestal (15) has some through holes; fix a microdialysis probe (1) in each through hole of pedestal (15); enter liquid protecting tube (9) by entering liquid sealing post (10) sealing; fluid protecting tube (13), by fluid sealing column (12) sealing, forms the annexation of series, parallel or grouping between each microdialysis probe (1).
2. array type microdialysis probe according to claim 1, it is characterized in that, the annexation of described series connection is specially: array type microdialysis probe also comprises some for connecting the connection protecting tube (11) of microdialysis probe (1), enter liquid protecting tube (9) and fluid protecting tube (13) is one, the liquid capillary tube (5) that enters of first microdialysis probe (1) passes into liquid protecting tube (9), after the fluid capillary tube (6) of first microdialysis probe (1) connects protecting tube (11) by first, with the liquid capillary tube (5) that enters of second microdialysis probe (1), communicate, after the fluid capillary tube (6) of second microdialysis probe (1) connects protecting tube (11) by second, with the liquid capillary tube (5) that enters of the 3rd microdialysis probe (1), communicate, the like, the fluid capillary tube (6) of last microdialysis probe (1) passes fluid protecting tube (13), enter liquid protecting tube (9), microdialysis probe (1), the junction connected between protecting tube (11) and fluid protecting tube (13) is connected by epoxy resin for connecting tube (8), entering liquid protecting tube (9), connection protecting tube (11) and fluid protecting tube (13) seals and is fixed on pedestal (15) by sealing epoxy resin (14).
3. array type microdialysis probe according to claim 1, it is characterized in that, the annexation of described parallel connection is specially: enter liquid protecting tube (9) and fluid protecting tube (13) is one, the liquid capillary tube (5) that enters of each microdialysis probe (1) passes respectively into liquid protecting tube (9), and the fluid capillary tube (6) of each microdialysis probe (1) passes same fluid protecting tube (13); The junction entered between liquid protecting tube (9), microdialysis probe (1) and fluid protecting tube (13) is connected by epoxy resin for connecting tube (8); Enter liquid protecting tube (9) and fluid protecting tube (13) seals and is fixed on pedestal (15) by sealing epoxy resin (14).
4. array type microdialysis probe according to claim 1; it is characterized in that; the annexation of described grouping is specially: the number that enters liquid protecting tube (9) and fluid protecting tube (13) is N; microdialysis probe (1) is divided into the N group; each organizes the length difference of microdialysis probe (1); the length of microdialysis probe (1) is identical on the same group; the number of every group of microdialysis probe (1) is 1 or is greater than 1; when the number of every group of microdialysis probe (1), when being greater than 1, each microdialysis probe (1) forms the annexation of serial or parallel connection.
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| CN 201110444617 CN102525569B (en) | 2011-12-28 | 2011-12-28 | Array type microdialysis probe |
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| CN 201110444617 CN102525569B (en) | 2011-12-28 | 2011-12-28 | Array type microdialysis probe |
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| CN102525569A CN102525569A (en) | 2012-07-04 |
| CN102525569B true CN102525569B (en) | 2013-12-25 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102809502A (en) * | 2012-08-21 | 2012-12-05 | 天津大学 | Microdialysis device for improving recovery rate of non-polar medicine |
| CN103308349A (en) * | 2013-06-09 | 2013-09-18 | 覃思 | Micro-dialysis sensor |
Citations (6)
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|---|---|---|---|---|
| US5882317A (en) * | 1996-09-20 | 1999-03-16 | Nec Corporation | Method and apparatus of sampling suction effusion fluid |
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| CN1891143A (en) * | 2005-07-07 | 2007-01-10 | 中国科学院生物物理研究所 | High-density electrode array and its making method |
| CN201286706Y (en) * | 2008-10-31 | 2009-08-12 | 中国人民解放军第二军医大学 | Intravascular sampling micro-dialysis probe |
| CN201822867U (en) * | 2009-11-23 | 2011-05-11 | 浙江大学 | Jugular vein micro dialysis probe and probe guide device for biological sampling |
| CN202408963U (en) * | 2011-12-28 | 2012-09-05 | 浙江省肿瘤医院 | Array type micro-dialysis probe |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7603153B2 (en) * | 2005-12-12 | 2009-10-13 | Sterling Investments Lc | Multi-element probe array |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5882317A (en) * | 1996-09-20 | 1999-03-16 | Nec Corporation | Method and apparatus of sampling suction effusion fluid |
| CN1358994A (en) * | 2000-12-18 | 2002-07-17 | 中国科学院化学研究所 | Sensor type microdialysis biological living sampling device |
| CN1891143A (en) * | 2005-07-07 | 2007-01-10 | 中国科学院生物物理研究所 | High-density electrode array and its making method |
| CN201286706Y (en) * | 2008-10-31 | 2009-08-12 | 中国人民解放军第二军医大学 | Intravascular sampling micro-dialysis probe |
| CN201822867U (en) * | 2009-11-23 | 2011-05-11 | 浙江大学 | Jugular vein micro dialysis probe and probe guide device for biological sampling |
| CN202408963U (en) * | 2011-12-28 | 2012-09-05 | 浙江省肿瘤医院 | Array type micro-dialysis probe |
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