CN201286706Y - Intravascular sampling micro-dialysis probe - Google Patents
Intravascular sampling micro-dialysis probe Download PDFInfo
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- CN201286706Y CN201286706Y CNU2008201547241U CN200820154724U CN201286706Y CN 201286706 Y CN201286706 Y CN 201286706Y CN U2008201547241 U CNU2008201547241 U CN U2008201547241U CN 200820154724 U CN200820154724 U CN 200820154724U CN 201286706 Y CN201286706 Y CN 201286706Y
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- dialyzer
- sampling
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Abstract
The utility model relates to a micro-dialysis probe for intravascular sampling, belonging to the technical field of biomedical micro-dialysis devices. The micro-dialysis probe comprises dialysis membrane tubes (1), a connecting pipe (2), an outer sleeve (3), a liquid outlet pipe (4) and a liquid inlet pipe (5). The micro-dialysis probe is characterized in that 1) scales indicating length are arranged on the outer wall of the outer sleeve to facilitate adjustment of the implanting depth of the probe; 2) the front end of the dialysis membrane tube is tied off with a silk thread for sealing, resulting in good sealing performance, high resistance to shock, low seepage liability after immersing in liquid for a long time, and prolonged service life of the probe; 3) the cutoff molecular weight range of the dialysis membrane tube is small, so as to reduce interference of large-molecular weight impurities; and 4) the dialysis membrane tubes are connected via the connecting pipe, so as to increase the difference between the inner diameter of the outer sleeve and the outer diameter of the dialysis membrane tube, and the liability of the dialysis membrane tubes contacting the vascular wall is reduced, which is in favor of dialysis and exchange. The micro-dialysis probe is, on the whole, linear, has certain rigidity, flexibility and good elasticity, and can freely bend, which facilitates probe implantation and sampling; and the implanting depth is adjustable, so as to enlarge the application range of the probe.
Description
Technical field
This utility model relates to a kind of biomedical micro-dialysis device technical field, is sampling microdialysis probe in a kind of blood vessel.
Background technology
Micro-dialysis device be based on micromolecule and water can be by dialyzer, spread and macromolecular substances can not see through a kind of biological living sampling device that dialyzer designs along Concentraton gradient.After the dialyzer probe is implanted animal body internal fixation zone, external micro pump makes the perfusate probe of flowing through, measured matter sees through dialyzer and diffuses into perfusate along concentration difference in the tissue, and reach a kind of dynamic equilibrium, by measuring the concentration of test substance in the effluent, the level of determinand and change procedure in research organization's extracellular fluid.
Microdialysis probe is the core of micro-dialysis device, various ways is arranged, roughly be divided into four classes by its structure: (1) concentric circular probe (concentric cannula probe), flexible stylet (flexible probe), shunting probe (shuntprobe), linear probe (linear probe).Wherein the concentric circular probe is comparatively commonly used, and has the following advantages: at first the internal diameter of probe is less, and is less to the damage of tissue; Because there is cecum, make the concentric circular probe be convenient to implant in addition,, be particularly useful for positions such as blood, spinal cord, the ventricles of the brain, articular cavity so the concentric circular probe can be used for many places samplings such as fat, tranquillization muscle, liver, gastrointestinal tract.
Existing commercial experiment concentric circular probe (concentric cannula probe), produce by offshore company, as the A-l-X-Y series probe of the CMA20Elite type of U.S. CMA company and Japanese EICOM company, by the trocar sheath of the dialyzer pipe of preceding end closure, regular length, be inserted to dialyzer pipe top feed tube, be inserted to the drain pipe at trocar sheath middle part and form at the holder of drain pipe, feed tube, trocar sheath intersection.According to the concrete condition that runs in the experiment, find that there is the deficiency of following several respects in these probes: at first, because the existence of holder, cause the implantable length of these probes to be fixed, can't regulate as required, for example the implantable length of CMA20Elite probe only is 2.4cm, and be the SD rat of 200-220g for body weight, in the microdialysis sampling determination blood during micromolecular compound (as medicine) concentration, probe must be inserted to right atrium through the right side external jugular vein, the implantation length of probe should be 3.0-3.5cm, but existing probe does not reach this requirement, and this just defines the selection of laboratory animal kind and body weight.Secondly, only than the big 0.22mm of external diameter of dialyzer, the external diameter difference is too small for the external diameter of trocar sheath, causes dialyzer to be easy to contact with blood vessel wall, the influence exchange, and then influence the response rate.The 3rd, the dialyzer molecular cut off of CMA20 Elite probe is 20,000 dalton, and the molecular cut off scope is bigger, if the collected sample of this probe only is used for micromolecular determination of drug concentration, then the endogenous material that molecular weight is bigger in the sample can produce measurement result and disturb.The 4th, the microdialysis Sampling techniques often need in conjunction with ultra high efficiency liquid phase quick analysis systems such as (UHPLC), and this is the advantage of this technology, yet because the column volume of UHPLC chromatographic column is less, if impurities is too much in the sample, can cause the quality overload of chromatographic column, thus impact analysis efficient and effect.
Domestic also do not have commercial microdialysis probe at present.A kind of patent No. is 96228271.5 " microdialysis probe ", form by the effuser of dialyzer pipe, interior pipe, trocar sheath, right angle bending and the outer shell that is positioned at the effuser three junction of pipe, trocar sheath, right angle bending, interior pipe that is feed tube, the effuser of right angle bending that is drain pipe.There is following some deficiency in this probe: at first, because the effuser of right angle bending and outer shell exist, make this probe have identical characteristics with above-mentioned such probe, promptly implant length and be fixed, limited the scope of its use; Secondly, the dialyzer front end of this probe seal the mode that adopts sealing compound to paste, impact resistance a little less than, be dipped in for a long time seepage take place in the liquid easily, influence the probe life-span.
Summary of the invention
The purpose of this utility model provide a kind of probe implant adjustable in length, dialyzer front end anti-impact force strong, can prevent sampling microdialysis probe in the less blood vessel of seepage, molecular cut off.
This utility model microdialysis probe is made up of dialyzer pipe, connection tube, trocar sheath, drain pipe and feed tube.Trocar sheath is placed in outside the feed tube, and its front end and connection tube join by binding agent is bonding, and tail end joins by binding agent and drain pipe, and the dialyzer pipe is adhesively fixed in the front end of connection tube by binding agent, the top seal of dialyzer pipe; Feed tube is one and has certain rigidity and flexible quartz capillary, its good springiness can free bend, is used to import dialysis solution, it places in the tube chamber of being made up of dialyzer pipe, connection tube, trocar sheath, and the top end opening place of feed tube is near dialyzer pipe top; Drain pipe and feed tube are adhesively fixed in the trocar sheath tail end by binding agent and are herringbone shape bifurcated.One of its feature is that the outer wall of trocar sheath is provided with the scale of expression length, so that regulate the probe implantation depth; Two of its feature is that the front end of dialyzer pipe adopts the silk thread ligation to be sealed, the reuse sealing compound is pasted the silk thread tieing, so both increased the impact resistance of dialyzer pipe front end, be dipped in for a long time and be not easy to take place seepage in the liquid, prolong the probe life-span, and can prevent that with sealing compound stickup silk thread tieing the silk thread tieing from loosening and the influence of tieing to implanting; Three of its feature is that the dialyzer molecular cut off is 5000 dalton, and the molecular cut off scope is little, can reduce the interference of macromolecule impurity, thereby reduces the quality overload of chromatographic column, and helping adopting fast, the UHPLC-MS analytical system detects; Four of its feature is that dialyzer pipe effective length can select as required, and scope is 2-30mm, thereby has enlarged the scope of application of probe; Five of its feature is to be connected by connection tube between dialyzer pipe and the trocar sheath, has widened the poor of trocar sheath internal diameter and dialyzer external diameter of pipe, and the dialyzer pipe is not easy to contact with blood vessel wall, helps the dialysis exchange.During use, routinely with the corresponding site of probe implantable intravascular, with micro pump perfusate is poured into probe by feed tube, perfusate is by dialyzer pipe front end flow through backward connection tube, trocar sheath and drain pipe, enter in the collecting pipe that is connected to the drain pipe mouth of pipe, just can measure the concentration of test substance in the effluent.
This utility model integral body is linear, has certain rigidity and flexibility, and good springiness can free bend, is convenient to the implantation and the sampling of probe, implants length and can regulate, and has enlarged the scope of application of probe, and sampling all can be used in the blood vessel of large and small type animal.The dialyzer front end adopts the ligation sealing tech, good airproof performance, and strong shock resistance is dipped in the liquid not easy to leak for a long time, prolongs the service life of probe.
Description of drawings
Fig. 1: this utility model microdialysis probe vertical section structure sketch map
The specific embodiment
Now in conjunction with the accompanying drawings and embodiments this utility model is described in detail.
This utility model microdialysis probe is made up of dialyzer pipe 1, connection tube 2, trocar sheath 3, drain pipe 4 and feed tube 5.Trocar sheath 3 is placed in outside the feed tube 5, and its front end and connection tube join by binding agent is bonding, and tail end joins by binding agent and drain pipe 4, and dialyzer pipe 1 is adhesively fixed in the front end of connection tube 2 by binding agent, the top seal of dialyzer pipe 1; Feed tube 5 is one and has certain rigidity and flexible quartz capillary, and its good springiness can free bend, and it places in the tube chamber of being made up of dialyzer pipe 1, connection tube 2, trocar sheath 3, close dialyzer pipe top, the top end opening place of feed tube; Drain pipe and feed tube are adhesively fixed by binding agent and are herringbone shape bifurcated in the trocar sheath tail end, and one of its feature is that the outer wall of trocar sheath is provided with the scale of expression length, so that regulate the probe implantation depth; Two of its feature is that the front end of dialyzer pipe 1 adopts the silk thread ligation to be sealed, and the reuse sealing compound is pasted the silk thread tieing; Three of its feature is that dialyzer pipe molecular cut off is 5000 dalton; Four of its feature is that dialyzer pipe effective length can select as required, and scope is 2-30mm, thereby has enlarged the scope of application of probe; Five of its feature is to be connected by connection tube between dialyzer pipe and the trocar sheath, has widened the poor of trocar sheath internal diameter and dialyzer external diameter of pipe, and the dialyzer pipe is not easy to contact with blood vessel wall, helps the dialysis exchange.
Embodiment 1: rat right atrium microdialysis probe
This utility model microdialysis probe length overall 11cm, dialyzer 1 material is a regenerated cellulose, effective length 10mm, internal diameter 0.2mm, external diameter 0.22mm, molecular cut off 5,000 dalton.The ligature of dialyzer pipe top seal is medical nylon monofilament line, be coated with the fixing residual silk thread two ends of sealing compound cyanoacrylate after the ligation, the binding agent that is used for being adhesively fixed between dialyzer pipe 1, connection tube 2, trocar sheath 3, drain pipe 4 and the feed tube 5 is a cyanoacrylate.Connection tube 2 adopts the quartz ampoule that scribbles the polyimides protective layer, internal diameter 0.25mm, external diameter 0.35mm, length 20mm.Trocar sheath 3 is the polypropylene capillary pipe, internal diameter 0.5mm, external diameter 0.8mm, length 40mm.Feed tube 5 outer walls scribble the polyimides protective layer, external diameter 0.15mm, and internal diameter 0.1mm, length 10cm, the top of feed tube 5 is apart from dialyzer pipe top 1mm.Trocar sheath 3 outer walls represent that the scale of length is 0～40mm.
Embodiment 2: rabbit right atrium microdialysis probe
This utility model microdialysis probe length overall 30cm, dialyzer 1 effective length 30mm, internal diameter 0.2mm, external diameter 0.22mm, trocar sheath 3 length 150mm, outer wall represent that the scale of length is 0～150mm.All the other are with embodiment 1.
This utility model can be made different specifications and models as required.
Claims (5)
1. sampling microdialysis probe in the blood vessel, it is characterized in that forming by dialyzer pipe (1), connection tube (2), trocar sheath (3), drain pipe (4) and feed tube (5), trocar sheath (3) is placed in outside the feed tube (5), its front end and connection tube join by binding agent is bonding, tail end joins by binding agent and drain pipe (4), dialyzer pipe (1) is adhesively fixed in the front end of connection tube (2) by binding agent, the top of dialyzer pipe (1) adopts the silk thread ligation to be sealed, and the reuse sealing compound is pasted the silk thread tieing; Feed tube (5) is one and has certain rigidity and flexible quartz capillary, it places in the tube chamber of being made up of dialyzer pipe (1), connection tube (2), trocar sheath (3), its top end opening place is near dialyzer pipe top, and drain pipe and feed tube are adhesively fixed in the trocar sheath tail end by binding agent and are herringbone shape bifurcated.
2. by sampling microdialysis probe in the described blood vessel of claim 1, it is characterized in that the outer wall of trocar sheath is provided with the scale of expression length.
3. by sampling microdialysis probe in claim 1 or the 2 described blood vessels, the molecular cut off that it is characterized in that dialyzer pipe (1) is 5000 dalton.
4. by sampling microdialysis probe in claim 1 or the 2 described blood vessels, the effective length that it is characterized in that dialyzer pipe (1) is 2-30mm.
5. by sampling microdialysis probe in the described blood vessel of claim 3, the effective length that it is characterized in that dialyzer pipe (1) is 2-30mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201547241U CN201286706Y (en) | 2008-10-31 | 2008-10-31 | Intravascular sampling micro-dialysis probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008201547241U CN201286706Y (en) | 2008-10-31 | 2008-10-31 | Intravascular sampling micro-dialysis probe |
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| Publication Number | Publication Date |
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| CN201286706Y true CN201286706Y (en) | 2009-08-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008201547241U Expired - Fee Related CN201286706Y (en) | 2008-10-31 | 2008-10-31 | Intravascular sampling micro-dialysis probe |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102525569A (en) * | 2011-12-28 | 2012-07-04 | 浙江省肿瘤医院 | Array type microdialysis probe |
| CN102809502A (en) * | 2012-08-21 | 2012-12-05 | 天津大学 | Microdialysis device for improving recovery rate of non-polar medicine |
| CN105125240A (en) * | 2015-07-21 | 2015-12-09 | 苏州百利医疗用品有限公司 | Medical disassembly-free sampling tube |
| EP3292884A1 (en) * | 2016-09-12 | 2018-03-14 | BIOTRONIK SE & Co. KG | A modified implantation tool tip configuration for the improved installation of leadless pacemakers with short tine-based anchors |
-
2008
- 2008-10-31 CN CNU2008201547241U patent/CN201286706Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102525569A (en) * | 2011-12-28 | 2012-07-04 | 浙江省肿瘤医院 | Array type microdialysis probe |
| CN102525569B (en) * | 2011-12-28 | 2013-12-25 | 浙江省肿瘤医院 | Array type microdialysis probe |
| CN102809502A (en) * | 2012-08-21 | 2012-12-05 | 天津大学 | Microdialysis device for improving recovery rate of non-polar medicine |
| CN105125240A (en) * | 2015-07-21 | 2015-12-09 | 苏州百利医疗用品有限公司 | Medical disassembly-free sampling tube |
| EP3292884A1 (en) * | 2016-09-12 | 2018-03-14 | BIOTRONIK SE & Co. KG | A modified implantation tool tip configuration for the improved installation of leadless pacemakers with short tine-based anchors |
| US11097114B2 (en) | 2016-09-12 | 2021-08-24 | Biotronik Se & Co. Kg | Modified implantation tool tip configuration for the improved installation of leadless pacemakers with short tine-based anchors |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090812 Termination date: 20171031 |