CN201263680Y - Flexible vascular micro dialysis probe for sampling from living body and guiding tube thereof - Google Patents

Flexible vascular micro dialysis probe for sampling from living body and guiding tube thereof Download PDF

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Publication number
CN201263680Y
CN201263680Y CNU2008201510942U CN200820151094U CN201263680Y CN 201263680 Y CN201263680 Y CN 201263680Y CN U2008201510942 U CNU2008201510942 U CN U2008201510942U CN 200820151094 U CN200820151094 U CN 200820151094U CN 201263680 Y CN201263680 Y CN 201263680Y
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China
Prior art keywords
feed tube
probe
inlet pipe
liquid inlet
drain pipe
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Expired - Fee Related
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CNU2008201510942U
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Chinese (zh)
Inventor
郑红
石力夫
胡晋红
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Second Military Medical University SMMU
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Second Military Medical University SMMU
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Priority to CNU2008201510942U priority Critical patent/CN201263680Y/en
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Abstract

The utility model relates to a flexible blood-vessel microdialysis probe and a guiding tube thereof for biological sampling. The probe consists of a tubular dialysis membrane (1), a liquid inlet pipe (2), a liquid outlet pipe (3) and an outer thimble (4), wherein the outer thimble is used for fixing the liquid inlet pipe and the liquid outlet pipe. The probe is characterized in that the liquid inlet pipe is divided into an upper liquid inlet pipe (21) and a lower liquid inlet pipe (22); one end of the upper liquid inlet pipe is fixed in the cavity of the upper end of the outer thimble; one end of the lower liquid inlet pipe is fixed in the cavity of the lower end of the outer thimble; the lengths of the lower liquid inlet pipe and the tubular dialysis membrane can be cut according to actual requirement so as to manufacture probes with different specifications. The lengths of the lower liquid inlet pipe and the tubular dialysis membrane are increased to enable the tubular dialysis membrane to reach the joint point of the right main line and the artery of heart, the main line at the position can provide abundant blood so as to effectively perform blood microdialysis and ensure good reproduction quality of the experiment results. The guiding tube (5) is matched with the probe, and the front opening of the guiding tube is provided with an inclined-plane-shaped incision so as to be conveniently placed in a blood vessel with high success ratio.

Description

Flexible blood vessel microdialysis probe and guiding tube thereof that a kind of living body sampling is used
Technical field
This utility model relates to a kind of biomedical micro-dialysis device technical field, is a kind of flexible blood vessel microdialysis probe and guiding tube thereof of biological living sampling usefulness.
Background technology
Micro-dialysis device be based on micromolecule and water can be by semipermeable membrane, spread and macromolecular substances can not see through a kind of biological living sampling device that semipermeable membrane designs along Concentraton gradient.When the semipermeable membrane probe being implanted in the animal body behind the specific region, external micro pump makes the perfusate probe of flowing through, measured matter sees through semipermeable membrane along the reverse perfusate that diffuses into of concentration difference in the tissue, and reach a kind of dynamic equilibrium, by measuring the concentration of determinand in the effluent, the level of determinand and change procedure in research organization's extracellular fluid.
Microdialysis probe is the core of micro-dialysis device, and it has various ways, roughly is divided into four classes by its structure: (1) concentric-ring pattern probe (concentric cannula probe); (2) flexible stylet (flexible probe); (3) linear probe (linear probe); (4) shunt probe (shunt probe) etc.Because the concentric-ring pattern probe is the rigid structure of shape such as pin, be difficult to be fixed on blood vessel and the internal's parenchima, moving of animal may cause the probe interior sleeve pipe to pierce through dialyzer or blood vessel wall, perhaps more seriously tear the tissue of implant site, thereby seldom be used for blood vessel or the interior tissue organ of animal.And the advantage of flexible stylet is that it has remedied the stiff problem of probe, is mainly used in blood sampling.
The MD-2305 type of existing flexible angio catheter such as U.S. BAS company, MD-2310 type, the TP-X-Y type of the CMA/20 MD type of Sweden CMA company and Japanese EICOM company has pliability.It is made of tubulose dialyzer, feed tube, drain pipe, trocar sheath.Trocar sheath hollow is used for fixing feed tube and drain pipe, and feed tube and drain pipe pass the trocar sheath inner chamber and be fixed.Dialyzer is connected in the lower end of feed tube, and end seals.Feed tube is two parallel quartz capillaries with drain pipe, and feed tube is thick, and drain pipe is thin, with the trocar sheath is the boundary, and on the trocar sheath, feed tube and drain pipe are separated from each other, so that the feed tube opening part links to each other with micro pump and is used to import perfusate, drain pipe is then exported dialysis solution and is used for detecting; Under the trocar sheath, drain pipe is inserted in the feed tube, and is inserted into the close sealing part of end of dialyzer always.During use, the animal jugular vein is isolated in operation routinely, with medical stainless steel syringe needle puncture vessel, and by medical stainless steel syringe needle importing guiding tube, after guiding tube is embedded in blood vessel, extract the medical stainless steel syringe needle out, insert flexible angio catheter by guiding tube again, and make dialyzer stretch out guiding tube to be dipped in outward in the blood, trocar sheath is positioned at outside the blood vessel.At this moment, can open the micro pump input perfusate that links to each other with the feed tube opening part, the related substances of perfusate in dialyzer place and blood interpenetrates, and drain pipe is then exported dialysis solution and is used for detecting.
There is following shortcoming in this flexible stylet: 1. specification is less, whole probe length is shorter, only be fit to be inserted into the jugular vein part, because jugular veins such as laboratory animal such as rat are very thin, tend to hinder blood flow after inserting microdialysis probe, not only cause vascular occlusion easily, but also can weaken blood circulation and microdialysis efficient; 2. do not have supporting guiding tube, to insert guiding tube, not only very likely puncture blood vessel, and operation is also inconvenient because of jugular vein is very thin with medical stainless steel syringe needle puncture vessel; 3. specification is less, has only two kinds of specifications of dialyzer length 5mm and 10mm, and the dialysis exchange area is little, for some experiment, often gathers the sample less than enough concentration, can't satisfy the needs of experiment; 4. cost an arm and a leg, a probe is wanted thousands of units, and the zoopery cost is too high.
Summary of the invention
The purpose of this utility model provides a kind of flexible blood vessel microdialysis probe of favorable reproducibility, multi-size, biological living sampling usefulness easy to operate, with low cost and guiding tube of implantable intravascular thereof of testing.
This utility model probe is made of tubulose dialyzer, feed tube, drain pipe, trocar sheath, and trocar sheath hollow is used for fixing feed tube and drain pipe; Dialyzer is connected in the lower end of feed tube, and end seals; Feed tube is two parallel capillary tubies with drain pipe, and feed tube is thick, and drain pipe is thin, with the trocar sheath is the boundary, and on the trocar sheath, feed tube and drain pipe are separated from each other, under the trocar sheath, drain pipe is inserted in the feed tube, and is inserted into the close sealing part of end of dialyzer always; It is characterized in that feed tube is divided into feed tube and following feed tube two parts, one end of last feed tube is fixed in the inner chamber of outer sleeve upper, the other end can link to each other with micro pump and be used to import perfusate, one end of following feed tube is fixed in the inner chamber of trocar sheath lower end, the other end links to each other with the tubulose dialyzer and communicates, the length of following feed tube and tubulose dialyzer can be cut out according to actual needs, and each joining place is adhesively fixed with the bio-compatibility binding agent respectively, thereby can be made into the probe of different size.The length of feed tube and tubulose dialyzer under particularly strengthening, make the tubulose dialyzer can arrive the junction point place of right large vein of heart and tremulous pulse, this place's large vein can provide abundant blood, obtain a bigger blood bank around making probe, the blood microdialysis is carried out effectively, the experimental result favorable reproducibility, the result is more reliable.
This utility model provides the guiding tube supporting with probe, and its length and following feed tube are same or slightly shorter, and internal diameter mates with the external diameter of following feed tube, the front opening place otch that takes the form of inclined plane.During use, the inclined plane shape otch directly can be inserted the jugular vein blood vessel of exposure, and be inserted into the junction point place of right large vein of heart and tremulous pulse along the large vein blood vessel, tubulose dialyzer one end with probe inserts guiding tube until the guiding tube top again, guiding tube is located along descending feed tube to retreat to the trocar sheath lower edge of probe, the tubulose dialyzer is promptly exposed, the experiment of just can taking a sample routinely.
This utility model self manufacture as required becomes different size, with low cost, easy to use, especially can strengthen the length of feed tube and tubulose dialyzer, make the tubulose dialyzer can arrive the junction point place of right large vein of heart and tremulous pulse, make the experimental result good reproducibility of blood microdialysis, the result is more reliable.Supporting probe guiding tube makes to operate when inserting blood vessel and becomes easy, the success rate height.
Description of drawings
Fig. 1 is a flexible blood vessel microdialysis probe structural representation of the present utility model.
Fig. 2 is a flexible blood vessel microdialysis probe vertical section structure sketch map of the present utility model.
Fig. 3 is a probe guiding tube structural representation of the present utility model.
The specific embodiment
Now in conjunction with the accompanying drawings and embodiments this utility model is described in detail.
Flexible blood vessel microdialysis probe of the present utility model is made of tubulose dialyzer 1, feed tube 2, drain pipe 3 and trocar sheath 4.Trocar sheath 4 hollows are used for fixing feed tube 2 and drain pipe 3; Dialyzer 1 is connected in the lower end of feed tube, and end seals; Feed tube is two parallel capillary tubies with drain pipe, and feed tube is thick, and drain pipe is thin, with the trocar sheath is the boundary, and on the trocar sheath, feed tube and drain pipe are separated from each other, under the trocar sheath, drain pipe is inserted in the feed tube, and is inserted into the close sealing part of end of dialyzer always; It is characterized in that feed tube 2 is divided into two-section up and down, promptly go up feed tube 21 and following feed tube 22, one end of last feed tube 21 is fixed in the inner chamber of trocar sheath 4 upper ends, the other end is used for linking to each other with micro pump, one end of following feed tube 22 is fixed in the inner chamber of trocar sheath 4 lower ends, and the other end links to each other with tubulose dialyzer 1 and communicates.
Tubulose dialyzer 1 external diameter 0.15-0.4mm of the present utility model, long 5-30mm, terminal with the bio-compatibility adhesive seal.The external diameter 0.2-0.7mm of following feed tube 22, the external diameter coupling of internal diameter and tubulose dialyzer 1, long 50-150mm.Last feed tube 21 external diameter 0.3-0.5mm, internal diameter 0.2-0.4mm, long 50-100mm.Trocar sheath 4 external diameter 0.6-0.8mm, internal diameter mates long 5-30mm with the external diameter of following feed tube 22.Drain pipe 3 external diameter 0.03-0.1mm, internal diameter 0.02-0.08mm, long 100-250mm.The joint all uses the bio-compatibility binding agent bonding, and the little probe length overall of analysing of flexible blood vessel of the present utility model is 100-250mm.
Probe guiding tube 5 of the present utility model is a plastics capillary flexible pipe, is used to overlap outside infra feed tube 22 and the tubulose dialyzer 1, and external diameter 0.6-0.8mm, internal diameter mates with the external diameter of following feed tube 22, long 50-100mm, the front end inclined-plane is to be 45 ° otch.When supporting, the length of guiding tube should be same or slightly shorter with the following feed tube 22 of flexible blood vessel microdialysis probe, at ordinary times guiding tube is placed in down outside the feed tube 22 and to dialyzer to be protected.
Embodiment 1: a kind of rat is with flexible blood vessel microdialysis probe and guiding tube thereof
Flexible blood vessel microdialysis probe length overall 150mm, the dialyzer material is a regenerated cellulose, long 10mm, internal diameter 0.2mm, external diameter 0.22mm, molecular cut off 5,000 dalton.Seal with resin glue the dialyzer lower end, last feed liquor bore 0.3mm, external diameter 0.5mm, long 80mm.Trocar sheath is made by polypropylene plastics, and external diameter 0.7mm, the external diameter of internal diameter and last feed tube add the external diameter coupling of drain pipe, long 10mm.Feed tube, drain pipe are quartz capillary, and outer wall all scribbles the polyimides protective layer.Following feed tube 22 internal diameter 0.3mm, external diameter 0.5mm, long 50mm; The internal diameter 0.04mm of drain pipe 3, external diameter 0.1mm, long 150mm.All junctions are all bonding with the cyanoacrylate instant drying adhesive.Guiding tube 5 is made by polypropylene plastics, external diameter 0.7mm, and internal diameter mates with the external diameter of following feed tube 22, and length is 50mm, and its front end inclined-plane is to be 45 ° otch.
Embodiment 2: a kind of rabbit is with flexible blood vessel microdialysis probe and guiding tube thereof
Flexible blood vessel microdialysis probe length overall 250mm, the material of dialyzer 1 is a Merlon, long 30mm, internal diameter 0.2mm, external diameter 0.22mm, molecular cut off 13,000 dalton.The end of dialyzer seals with resin glue, and last feed tube 21 is made internal diameter 0.3mm, external diameter 0.5mm, long 120mm by polypropylene plastics.Trocar sheath 4 is made by polypropylene plastics, external diameter 0.7mm, and internal diameter mates long 20mm with the external diameter of following feed tube 22.Following feed tube, drain pipe are quartz capillary, and outer wall all scribbles the polyimides protective layer.Following feed tube 22 internal diameter 0.3mm, external diameter 0.5mm, long 80mm; Drain pipe internal diameter 0.04mm, external diameter 0.1mm, long 250mm.All junctions are all bonding with the cyanoacrylate instant drying adhesive.Guiding tube is made by polypropylene plastics, external diameter 0.7mm, and internal diameter mates with the external diameter of following feed tube 22, and length is 75mm, and its front end inclined-plane is to be 45 ° otch.
This utility model can be made different specifications and models as required.

Claims (3)

1. a flexible blood vessel microdialysis probe is made of tubulose dialyzer (1), feed tube (2), drain pipe (3) and trocar sheath (4), and trocar sheath (4) hollow is used for fixing feed tube and drain pipe; Dialyzer is connected in the lower end of feed tube, and end seals; Feed tube is thick, and drain pipe is thin, is the boundary with the trocar sheath, and on the trocar sheath, feed tube and drain pipe are separated from each other, and under the trocar sheath, drain pipe is inserted in the feed tube, and is inserted into the close sealing part of end of dialyzer always; It is characterized in that feed tube is divided into feed tube (21) and following feed tube (22) two parts, one end of last feed tube is fixed in the inner chamber of outer sleeve upper, the other end is used for linking to each other with micro pump, one end of following feed tube is fixed in the inner chamber of trocar sheath lower end, the other end links to each other with the tubulose dialyzer and communicates, the length of following feed tube and tubulose dialyzer is cut out according to actual needs, and the joint all uses the bio-compatibility binding agent bonding.
2. by the described flexible blood vessel microdialysis probe of claim 1, it is characterized in that also being provided with the guiding tube of probe, the length of this guiding tube is same or slightly shorter with following feed tube, and internal diameter mates with the external diameter of following feed tube, the front opening place otch that takes the form of inclined plane.
3. by the described flexible blood vessel microdialysis probe of claim 2, the inclined-plane at feed tube front opening place is for being 45 under it is characterized in that 0Otch.
CNU2008201510942U 2008-07-22 2008-07-22 Flexible vascular micro dialysis probe for sampling from living body and guiding tube thereof Expired - Fee Related CN201263680Y (en)

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CNU2008201510942U CN201263680Y (en) 2008-07-22 2008-07-22 Flexible vascular micro dialysis probe for sampling from living body and guiding tube thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNU2008201510942U CN201263680Y (en) 2008-07-22 2008-07-22 Flexible vascular micro dialysis probe for sampling from living body and guiding tube thereof

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102809502A (en) * 2012-08-21 2012-12-05 天津大学 Microdialysis device for improving recovery rate of non-polar medicine
CN105148377A (en) * 2015-07-21 2015-12-16 苏州百利医疗用品有限公司 Spiral core sampling tube for medical use
CN111450350A (en) * 2019-01-22 2020-07-28 华东师范大学 Venous indwelling needle, system and method capable of continuously supplementing and sampling liquid based on microdialysis

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102809502A (en) * 2012-08-21 2012-12-05 天津大学 Microdialysis device for improving recovery rate of non-polar medicine
CN105148377A (en) * 2015-07-21 2015-12-16 苏州百利医疗用品有限公司 Spiral core sampling tube for medical use
CN105148377B (en) * 2015-07-21 2018-07-27 苏州百利医疗用品有限公司 A kind of medical spiral core sampling pipe
CN111450350A (en) * 2019-01-22 2020-07-28 华东师范大学 Venous indwelling needle, system and method capable of continuously supplementing and sampling liquid based on microdialysis

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090701

Termination date: 20170722