CN108246373A - Centrifugal type microfludic chip - Google Patents
Centrifugal type microfludic chip Download PDFInfo
- Publication number
- CN108246373A CN108246373A CN201810188633.8A CN201810188633A CN108246373A CN 108246373 A CN108246373 A CN 108246373A CN 201810188633 A CN201810188633 A CN 201810188633A CN 108246373 A CN108246373 A CN 108246373A
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- 239000000758 substrate Substances 0.000 claims abstract description 31
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 239000011148 porous material Substances 0.000 claims abstract description 6
- 230000000149 penetrating effect Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 abstract description 16
- 238000005119 centrifugation Methods 0.000 abstract description 7
- 238000009826 distribution Methods 0.000 abstract description 5
- 238000012545 processing Methods 0.000 abstract description 5
- 239000007795 chemical reaction product Substances 0.000 abstract description 4
- 238000009792 diffusion process Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 30
- 238000005516 engineering process Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000004308 accommodation Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- -1 electronics Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention provides a kind of simple and reasonable, save the centrifugal type microfludic chip of processing cost, cover plate including substrate and with substrate close fit, the substrate and the main channel that several circumferentially distributed reacting holes and at least one circumferentially distributed several reacting holes of connection are offered on the end face of cover plate cooperation, main channel described in every is the waveform channel with multiple wave crests and multiple troughs, the reacting hole sets that there are one the interface channels towards substrate center, interface channel is connected with the trough of main channel, reacting hole is connected by interface channel with main channel, main channel between the adjacent reacting hole of each two is equipped with fluid-tight unit.The centrifugal type microfludic chip of the present invention, based on U-tube fluid-tight principle, ensure that liquid is full up in reacting hole after centrifugation distribution, prevent reaction product in each reacting hole to adjacent reaction pore diffusion, make flow rate of liquid in main channel uniform, realize that sample more uniformly distributes.
Description
Technical field
The present invention relates to a kind of micro-fluidic chip, more particularly to a kind of centrifugal type microfludic chip, belong to life science and
Medical science.
Background technology
Microfluidic chip technology is bases such as biology, chemistry, the sample preparation of medical analysis process, reaction, separation, detections
This operating unit is integrated on the chip of one piece of micro-meter scale, is automatically performed analysis overall process.Since it is in biology, chemistry, doctor
The great potential in etc. fields has been developed as the friendship of the subjects such as biology, chemistry, medicine, fluid, electronics, material, a machinery
The brand-new research field of fork.Microfluidic chip technology provides a variety of realization platforms of monomolecular nucleic acid detection method, by micro-
Mechanical manufacturing technology is integrated in the analysis operation of biological sample on several square centimeters of micro-fluidic chip.It is small using volume
Centrifugal type microfludic chip, the centrifugal force treatment fluid generated by motor rotation plate-like chip, not only greatly reduces analysis
Cost, and detection time is short, high sensitivity, it is easy to carry, biochemical analyzer is led to intelligent and miniaturization.
The patent document of Publication No. CN103831140A discloses a kind of micro-fluidic chip of multiple determination, the chip
Including one or more waveform main channel, by connecting pipe connected reaction pond, connecting pipe is equipped with the trough of main channel
Buffer pool.The chip by buffer pool ensure liquid remains full of in after centrifugation distribution and entire heating reaction present invention pond,
The reaction product in each reaction tank is prevented to be spread into main channel and adjacent reaction pond.But its there are drops during sample introduction easily to splash
Sample cross contamination is caused to chip surface, the problem of sample introduction liquid is easily overflowed from gas vent.
The patent document of Publication No. CN205379906U discloses a kind of multipurpose micro-fluidic chip, the egative film of the chip
On offer one or more corrugated main channel, the cross-sectional area at the wave crest of main channel is less than or equal to the cross section at trough
Street, one end connection sample holes of each main channel, other end connection long capillary road and gas vent, main channel and long capillary road
Junction be equipped with liquid level blocking unit, the cross-sectional area of liquid level blocking unit is more than main channel in communication and long capillary
The cross-sectional area in road, the trough of each main channel connects a reaction tank, between the two equipped with a buffer pool.The chip is using centrifugation
The mode of sample introduction evenly distributes detected sample, realizes that sample more uniformly divides by the ratio for limiting main channel sectional area
Match, it is full up by liquid in reaction tank after the design guarantee centrifugation distribution of buffer pool, and prevent the reaction product in each reaction tank
It is spread into main channel and adjacent reaction pond.But the wide channel cross-section design of the chip main channel narrow trough of wave crest influences main channel
The flow velocity of middle liquid, it is impossible to be fully solved bubble in the problem of sample evenly distributes and main channel and lead to the problem of, and increase
The complexity and processing cost of chip manufacturing.
Invention content
The purpose of the present invention is to provide it is a kind of it is simple and reasonable, save processing cost centrifugal type microfludic chip,
It solves the problems, such as to propose in background above technology.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of centrifugal type microfludic chip, the cover plate including substrate and with substrate close fit, the substrate coordinate with cover plate
End face on offer the masters of several circumferentially distributed reacting holes and at least one circumferentially distributed several reacting holes of connection
Channel；Main channel described in every is the waveform channel with multiple wave crests and multiple troughs, and each wave crest is close to substrate
The direction at center, each trough direction far from substrate center；One end of main channel described in every is with being set on substrate and passing through
The step-like sample holes connection of logical cover plate, the other end are connected with being set on substrate and penetrating through the gas vent of cover plate；It is anti-described in each
There are one the interface channels towards substrate center by Ying Kongshe, and interface channel to connect with the trough of main channel, and reacting hole passes through connection
Channel is connected with main channel；Main channel between the adjacent reacting hole of each two is equipped with fluid-tight unit.
Preferably, the main channel between the adjacent reacting hole of each two includes at least a trough and two waves
Peak, a trough of main channel and two wave crests form a fluid-tight unit.
Preferably, it is equal at each wave crest of the main channel with the cross-sectional area at each trough, main channel
Cross-sectional area be greater than or equal to interface channel cross-sectional area.
Preferably, each wave crest and the distance of substrate center are equal, each trough and substrate center away from
From being equal.
Preferably, the quantity of the reacting hole is 10 ~ 24, the volume of each reacting hole is 5 ~ 25 μ L, each two
The volume of main channel between adjacent reacting hole is at least 2 times of each reaction pore volume.
Preferably, the central point of the reacting hole, the tie point of interface channel and reacting hole, interface channel lead to main
The tie point of road trough and substrate center's point are respectively positioned on same line.
Preferably, the sample holes are the stepped hole being made of a macropore and aperture stacking, macropore and aperture are same
The heart, big hole depth be 0.6 ~ 1mm, small hole depth be 0.1 ~ 0.4mm, diameter macropores be 1 ~ 5mm, hole diameter be 1 ~ 3mm, macropore
Cross-sectional area is 2 ~ 6 times of aperture cross-sectional area.
Preferably, the sample holes and the distance and gas vent and the distance of main channel end of main channel end are big
Vertical range between the adjacent peaks of main channel and trough.
Preferably, the sample holes and the distance of substrate center are less than the distance of gas vent and substrate center, exhaust
Bore dia is 1 ~ 3mm.
Preferably, the center of the centrifugal type microfludic chip is equipped with central through hole, central through hole through substrate and
Cover plate.
The beneficial effects of the invention are as follows：
The centrifugal type microfludic chip of the present invention, based on U-tube fluid-tight principle, by spaced reacting hole, makes each two
Main channel between adjacent reaction hole includes at least a trough and two wave crests, at each wave crest with it is transversal at each trough
Area is equal, and forms fluid-tight unit with this, liquid is full up in reacting hole after guarantee centrifugation distribution, prevents anti-in each reacting hole
Product is answered to adjacent reaction pore diffusion, and due to the equal design of the Wave crest and wave trough channel cross-section of main channel, makes main channel
Interior flow rate of liquid is uniform, realizes that sample more uniformly distributes, also simple and convenient and save processing cost with processing and fabricating
Advantage in addition, by setting step-like sample holes, is realized the no sample drop leakage that chip is loaded into during sample operation, is kept away
Exempt from sample cross contamination.
Description of the drawings
Fig. 1 is the dimensional structure diagram of the present invention；
Fig. 2 is the schematic front view of substrate of the present invention；
Fig. 3 is the schematic front view of cover plate of the present invention.
In figure：1st, substrate, 2, cover plate, 3, central through hole, 4, reacting hole, 5, main channel, 6, wave crest, 7, trough, 8, sample introduction
Hole, 9, gas vent, 10, macropore, 11, aperture, 12, interface channel, 13, fluid-tight unit.
Specific embodiment
Below by specific embodiment, and with reference to attached drawing, technical scheme of the present invention is described in further detail.It should
Work as understanding, implementation of the invention is not limited to the following examples, the accommodation in any form done to the present invention and/or
Change falls within the scope of the present invention.
Embodiment：
A kind of centrifugal type microfludic chip as shown in Figure 1, the cover plate 2 including substrate 1 and with substrate close fit, substrate and lid
Piece is circle, and substrate thickness 3mm, coverslip thickness 1mm, equipped with central through hole 3, center leads at this centrifugal type microfludic chip center
Substrate and cover plate are run through in hole.As shown in Fig. 2, substrate on the end face of cover plate cooperation with offering 21 circumferentially distributed reacting holes
4 connect 7 reacting holes with 3 circumferentially distributed main channels 5 for connecting above-mentioned reacting hole, every main channel.Each reacting hole
Volume for 25 μ L, 2 times for each reaction pore volume of the volume of the main channel between the adjacent reacting hole of each two.Centrifugation
Fluidic chip decline before use, the object of row differential responses detection can be loaded into differential responses hole in advance as needed
Matter, to realize a variety of reactive applications, such as amplification, biochemistry or immune reaction.
Every main channel is the waveform channel with multiple wave crests 6 and multiple troughs 7, in the present embodiment, every master
The wave crest of channel is 14, and trough is 15.Each wave crest is close to the direction of substrate center, and each trough is far from substrate
The direction of the heart.The distance of each wave crest and substrate center is equal, and the distance of each trough and substrate center is equal.
One end of every main channel is connected with being set on substrate and penetrating through the step-like sample holes 8 of cover plate, and the other end is with being set on
Substrate simultaneously penetrates through the gas vent 9 of cover plate and connects.Entire substrate is equipped with 3 sample holes and 3 gas vents, respectively with 3 along week
Coordinate to the main channel of distribution.
As shown in figure 3, sample holes are the stepped hole being made of a macropore 10 and an aperture 11 stacking, macropore and aperture are same
The heart, big hole depth be 0.8mm, small hole depth be 0.2mm, diameter macropores 3mm, hole diameter 1.5mm, macropore cross-sectional area
It is 4 times of aperture cross-sectional area.Sample holes and the distance and gas vent and the distance of main channel end of main channel end are all higher than
Vertical range between the adjacent peaks and trough of main channel.Sample holes and the distance of substrate center are less than gas vent and substrate center
Distance.A diameter of 1 ~ the 3mm of gas vent.After centrifugal type microfludic chip sample introduction, using the sealed membrane for having viscosity to sample introduction
Hole and gas vent are closed, and sample is made to be completely sealed in micro-fluidic chip.
Each reacting hole is set there are one the interface channel 12 towards substrate center, and the trough of interface channel and main channel connects
Logical, reacting hole is connected by interface channel with main channel.The central point of reacting hole, connects the tie point of interface channel and reacting hole
The tie point and substrate center's point for connecting road and main channel trough are respectively positioned on same line.It is at each wave crest of main channel and every
Cross-sectional area at a trough is equal, and the cross-sectional area of main channel is equal to the cross-sectional area of interface channel.
Main channel between the adjacent reacting hole of each two is equipped with fluid-tight unit 13, specifically, adjacent anti-of each two
The main channel between hole is answered to include a trough and two wave crests, a trough of main channel and two wave crests form a fluid-tight
Unit.Based on U-tube fluid-tight principle, fluid-tight unit so that liquid is full up in reacting hole after centrifugation distributes, and prevents each reacting hole
Interior reaction product is to adjacent reaction pore diffusion.
This centrifugal type microfludic chip when in use, is first loaded chip by sample holes, and sample-adding liquid fills main logical
In road, since sample holes are step-like, the liquid for remaining in sample adding device adding mouth edge is accommodated by macropore, does not have liquid drop
Drain to chip surface；After sample-adding, well and gas vent are sealed using sealed membrane.Centrifugally operated is carried out to chip, makes main channel
Interior sample-adding liquid is assigned in each reacting hole, due to the main channel between each two adjacent reaction hole and the volume of reacting hole
Ratio is appropriate, has sufficient liquid measure in reacting hole, ensures the accuracy of test result.
Embodiment described above is a kind of preferable scheme of the present invention, and not the present invention is made in any form
Limitation also has other variants and remodeling under the premise of without departing from the technical solution recorded in claim.
Claims (10)
1. a kind of centrifugal type microfludic chip, it is characterised in that：The centrifugal type microfludic chip includes substrate and closed with substrate
The cover plate of cooperation, the substrate on the end face of cover plate cooperation with offering several circumferentially distributed reacting holes and at least one
The main channel of circumferentially distributed several reacting holes of connection；
Main channel described in every is the waveform channel with multiple wave crests and multiple troughs, and each wave crest is close in substrate
The direction of the heart, each trough direction far from substrate center；
One end of main channel described in every is connected with being set on substrate and penetrating through the step-like sample holes of cover plate, and the other end is with being set on
Substrate and the gas vent connection for penetrating through cover plate；
The reacting hole is set there are one the interface channel towards substrate center, and the trough of interface channel and main channel connects
Logical, reacting hole is connected by interface channel with main channel；
Main channel between the adjacent reacting hole of each two is equipped with fluid-tight unit.
2. centrifugal type microfludic chip according to claim 1, it is characterised in that：The adjacent reacting hole of each two
Between main channel include at least a trough and two wave crests, a trough of main channel and two wave crests form a fluid-tights
Unit.
3. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：Each wave of the main channel
It is equal at peak with the cross-sectional area at each trough, the cross-sectional area of main channel is greater than or equal to the cross section of interface channel
Product.
4. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：Each wave crest and substrate
The distance at center is equal, and the distance of each trough and substrate center is equal.
5. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：The quantity of the reacting hole is
10 ~ 24, the volume of each reacting hole is 5 ~ 25 μ L, and the volume of the main channel between the adjacent reacting hole of each two is at least
2 times of each reaction pore volume.
6. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：The central point of the reacting hole,
The tie point of interface channel and reacting hole, the tie point of interface channel and main channel trough and substrate center's point are respectively positioned on same company
On line.
7. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：The sample holes are big by one
The stepped hole of composition is laminated in hole and an aperture, and macropore and aperture are concentric, and big hole depth is 0.6 ~ 1mm, small hole depth for 0.1 ~
0.4mm, diameter macropores are 1 ~ 5mm, and hole diameter is 1 ~ 3mm, and macropore cross-sectional area is 2 ~ 6 times of aperture cross-sectional area.
8. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：The sample holes and main channel
The distance and gas vent of end and the distance of main channel end are all higher than the vertical range between the adjacent peaks of main channel and trough.
9. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：In the sample holes and substrate
The distance of the heart is less than the distance of gas vent and substrate center, a diameter of 1 ~ 3mm of gas vent.
10. centrifugal type microfludic chip according to claim 1 or 2, it is characterised in that：The centrifugal type microfludic core
The center of piece is equipped with central through hole, and central through hole runs through substrate and cover plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810188633.8A CN108246373B (en) | 2018-03-07 | 2018-03-07 | Centrifugal micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810188633.8A CN108246373B (en) | 2018-03-07 | 2018-03-07 | Centrifugal micro-fluidic chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108246373A true CN108246373A (en) | 2018-07-06 |
| CN108246373B CN108246373B (en) | 2024-05-10 |
Family
ID=62746055
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810188633.8A Active CN108246373B (en) | 2018-03-07 | 2018-03-07 | Centrifugal micro-fluidic chip |
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| Country | Link |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112755933A (en) * | 2021-01-13 | 2021-05-07 | 广东工业大学 | Multi-stage reaction micro-channel structure, micro-fluidic chip and heterogeneous reaction method |
| CN115155678A (en) * | 2022-06-09 | 2022-10-11 | 安图实验仪器(郑州)有限公司 | Centrifugal micro-fluidic analysis chip |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040121449A1 (en) * | 2002-12-19 | 2004-06-24 | Pugia Michael J. | Method and apparatus for separation of particles in a microfluidic device |
| JP2012185000A (en) * | 2011-03-04 | 2012-09-27 | Toppan Printing Co Ltd | Sample analysis chip and sample analysis method using the same |
| CN205379906U (en) * | 2016-02-19 | 2016-07-13 | 博奥生物集团有限公司 | Multipurpose micro -fluidic chip |
| CN208177461U (en) * | 2018-03-07 | 2018-12-04 | 杭州殷欣病理诊断中心有限公司 | Centrifugal type microfludic chip |
-
2018
- 2018-03-07 CN CN201810188633.8A patent/CN108246373B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040121449A1 (en) * | 2002-12-19 | 2004-06-24 | Pugia Michael J. | Method and apparatus for separation of particles in a microfluidic device |
| JP2012185000A (en) * | 2011-03-04 | 2012-09-27 | Toppan Printing Co Ltd | Sample analysis chip and sample analysis method using the same |
| CN205379906U (en) * | 2016-02-19 | 2016-07-13 | 博奥生物集团有限公司 | Multipurpose micro -fluidic chip |
| CN208177461U (en) * | 2018-03-07 | 2018-12-04 | 杭州殷欣病理诊断中心有限公司 | Centrifugal type microfludic chip |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112755933A (en) * | 2021-01-13 | 2021-05-07 | 广东工业大学 | Multi-stage reaction micro-channel structure, micro-fluidic chip and heterogeneous reaction method |
| CN112755933B (en) * | 2021-01-13 | 2023-12-26 | 广东工业大学 | Multistage reaction micro-channel structure, micro-fluidic chip and heterogeneous reaction method |
| CN115155678A (en) * | 2022-06-09 | 2022-10-11 | 安图实验仪器(郑州)有限公司 | Centrifugal micro-fluidic analysis chip |
| WO2023236787A1 (en) * | 2022-06-09 | 2023-12-14 | 安图实验仪器(郑州)有限公司 | Centrifugal microfluidic analysis chip |
| CN115155678B (en) * | 2022-06-09 | 2024-02-09 | 安图实验仪器(郑州)有限公司 | Centrifugal microfluidic analysis chip |
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| CN108246373B (en) | 2024-05-10 |
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