CN205379906U - Multipurpose micro -fluidic chip - Google Patents
Multipurpose micro -fluidic chip Download PDFInfo
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- CN205379906U CN205379906U CN201620128203.3U CN201620128203U CN205379906U CN 205379906 U CN205379906 U CN 205379906U CN 201620128203 U CN201620128203 U CN 201620128203U CN 205379906 U CN205379906 U CN 205379906U
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Abstract
The utility model relates to a multipurpose micro -fluidic chip, this chip include a film and one and the sealed complex cover plate of film, have offered corrugated main entrance more than or on the film, and the main entrance arranges on the circumference that uses the plate center as the centre of a circle that each main entrance all includes a plurality of crests and trough, and film center directions is all all kept away from near film center directions, trough to the crest, the one end of each main entrance and a big -end -up's step form injection hole are linked together, the other end is linked together through a long capillary channel and an exhaust hole, and injection hole, long capillary channel and exhaust hole all set up on the film, the junction of each main entrance and long capillary channel all is provided with a liquid level and blocks the unit, the trough of each main entrance all is linked together through a connecting tube and a reaction tank, all is provided with a buffer pool on each connecting tube, and connecting tube, reaction tank and buffer pool also set up on the film, the cover plate seals with the structure the exhaust hole except that injection hole on to the film.
Description
Technical field
This utility model relates to a kind of life sciences and medical detection device, particularly relates to a kind of multipurpose micro-fluidic chip.
Background technology
Micro-fluidic chip, with whole chip for operating platform, by the combination with technology such as biology, chemistry, drug screenings, completes to include the whole sample analysis process of reagents loaded, separation, reaction, detection etc..Micro-fluidic chip is micro-total analysis system (MicroTotalAnalysisSystems, μ-TAS) hot fields that develops, in recent years, along with the fast development of biochip technology, micro-fluidic chip plays more and more important effect at life sciences, analytical chemistry and biomedical sector.For detection sample efficient, quick, high-throughout, it is desirable to micro-fluidic chip has many group reactions hole and carries effective mode of movement of sample to each reacting hole.At present, testing sample is generally delivered to chip internal by the External Force Acting such as electromagnetic force, centrifugal force by micro-fluidic chip.
The patent documentation that publication number is CN101609088A discloses under the effect of external electrical field, and charged drop is applied electric field force, controls the flow sending method that micropartical moves in microfluidic channel to each stub area.But, the method needs to introduce the complex mechanism generating electric field and equipment in chip, and carries liquid to need to be first converted into drop at electric field region, then to specifying region to send stream in the way of drop, greatly reduces the processing speed of sample.
The patent documentation that publication number is CN103055973A discloses the electroosmotic pump utilizing driving sample, the method separated by charged different test substance.But the method is only applicable to the sample with electric charge, general biological sample and uncharged sample is inoperative.
The patent documentation that publication number is CN102369443A discloses a kind of centrifugal infusion chip, and namely liquid is centrifuged dispensing from storage part each sample holes peripherad of chip central authorities.The mountain portion of this chip and valley are with wide primary flow path design, the problem failing to solve each sample holes dosing uniformity；And the design that valley is more wide than mountain portion, then cause the problem that in sample introduction process, primary flow path has bubble to produce.In addition, the solution excessive by adding waste liquid chamber carrying, though achieving the concordance of each sample holes distribution liquid volume to a certain extent, but it cannot be guaranteed that liquid is full of sample holes all the time in course of reaction, and need inside microfluidic channel, carry out local surfaces modification, namely hydrophilic treated is carried out at the branch flow passage inner surface being connected with sample holes, hydrophobic treatment is being carried out, thus adding the complexity of chip manufacturing and processing cost and difficulty of processing with the branch flow passage inner surface that is connected of waste liquid chamber collecting surplus solution.
Publication number is the micro-fluidic chip that the patent documentation of CN103831140A discloses a kind of multiple determination, and this chip includes one or more corrugated main channel, and one end of every main channel is connected with sample holes, and the other end is connected with steam vent；The trough of described main channel arranges away from chip center direction and is connected by connecting pipeline and a reaction tank, is provided with Buffer Pool connecting on pipeline.Though this micro-fluidic chip achieves to the homogeneous dispensed liquid sample of each sample holes by limiting the ratio of waveform main channel cross section, after having ensured centrifugal distribution by the design of Buffer Pool and during whole reacting by heating, in reaction tank, liquid remains full of, stops the product in each reaction tank to main channel and adjacent reaction pond internal diffusion, but when there is sample introduction, drop easily splashes the problem that chip surface, sample introduction liquid easily overflow from steam vent.The drop caused during sample introduction leaks outside, following two problem can be caused: if the independent detection region that 1. a chip comprises multiple sample, and the inlet and outlet in adjacent independent detection region is by relatively near, then the drop leaked outside during sample introduction easily enters adjacent detection area, thus causing cross-contamination；2. after sample introduction, water clock is to the drop of chip surface, if not carrying out wiping, directly with one side glue be sealed into, outlet, then bonding plane can be caused insecure, thus causing seal bulge in reacting by heating process, even the phenomenon such as breaking.
Summary of the invention
For the problems referred to above, the purpose of this utility model is to provide a kind of multipurpose micro-fluidic chip, simple in construction, easily manufactured, not only realize to the accurate dosing of each sample holes, avoid cross-contamination between contiguous reaction tank, and realize no liquid water clock to chip surface, no liquid from steam vent spilling, without wiping, thus reaching simplicity, quick, high flux and high-precision requirement.
For achieving the above object, this utility model takes techniques below scheme: a kind of multipurpose micro-fluidic chip, it is characterized in that, this chip includes an egative film and and seals, with described egative film, the cover plate coordinated, described egative film offers one or more corrugated main channel, described main channel is arranged in described plate center for the circumference in the center of circle, each described main channel all includes multiple crest and trough, and described crest is close to described plate center direction, described trough all away from described plate center direction；The step-like sample holes that one end of each described main channel is up big and down small with one is connected, the other end is connected by a long capillary road and a steam vent, and described sample holes, long capillary road and steam vent may be contained within described egative film；The junction in each described main channel and described long capillary road is provided with a liquid level blocking unit, and the cross-sectional area of described liquid level blocking unit is more than the cross-sectional area being attached thereto logical described main channel and long capillary road；The trough of each described main channel connects pipeline each through one and a reaction tank is connected, and each described connection pipeline is provided with a Buffer Pool, and described connection pipeline, reaction tank and Buffer Pool are also arranged on described egative film；Structure except described sample holes and steam vent on described egative film is sealed by described cover plate.
The cross-sectional area at described crest place is 0.2～1 less than or equal to the ratio of the cross-sectional area at described trough place, the cross-sectional area at the narrowest place, described main channel and the cross-sectional area of the widest part.
Described sample holes is the stepped hole being made up of a macropore and an aperture, the center superposition of the center of described macropore and described aperture；The cross-sectional area of described macropore is 4～50 times of the cross-sectional area of described aperture, and the degree of depth of described macropore is 40 μm～800 μm；Described aperture is circular, and the size of described aperture matches with the size of conventional use of standard Tip head in biotic experiment；The shape of described macropore is circular, oval or square.
Distance between described steam vent and described sample holes is not more than 10mm.
The shape of described liquid level blocking unit is semicircle, oval, circular or square.
The number of described reaction tank is 5～100, and the volume of each described reaction tank is 0.1～25 μ L；The volume of the peak-to-peak one section of V-arrangement main channel of ripple described in adjacent two is 1.2～1.8 times of the volume of coupled logical described reaction tank；The volume of described Buffer Pool is 0.2～0.8 times of the volume of described reaction tank.
The multiple described reaction tank being connected with main channel described in same is one group, be additionally provided with a waste liquid pool between one section of described main channel and the described liquid level blocking unit corresponding with last described reaction tank of one group, the second thin pipe that the first thin pipe that described waste liquid pool includes being connected with described main channel is connected with described liquid level blocking unit and between described first thin pipe and the second thin pipe for carrying the waste liquid Buffer Pool of liquid；The volume of described waste liquid pool is not less than the volume of one section of described V-arrangement main channel.
The thickness of described egative film and cover plate is 0.05～5mm；The degree of depth of described main channel, reaction tank, Buffer Pool and connection pipeline is 40 μm～4mm.
Described chip center is provided with central through hole, and the inward flange of described central through hole or the outer edge of described chip are provided with at least one locating notch；Or, at least one hole, location it is provided with in the optional position of described chip；Described micro-fluidic chip is additionally provided with sample partition identification.
The material for carrying out nucleic acid amplification reaction, biochemical reaction or immunoreation detection it is pre-loaded with respectively in different described reaction tanks；Or, in different described reaction tanks, it is pre-loaded with the different material for same reaction detection respectively.
This utility model is owing to taking above technical scheme, and it has the advantage that 1, multipurpose micro-fluidic chip of the present utility model, by the sample holes of setting table scalariform, it is achieved during chip sample introduction, dripless leaks outside, without wiping after sample introduction；Shorten the distance between sample holes and steam vent by arranging long capillary, save sealed membrane raw material, simplify user operation step, and make chip outward appearance more attractive in appearance；By arranging liquid level blocking unit between long capillary and main channel, solve in main channel liquid easily by capillary to long capillary, the problem even overflowed from steam vent, it is to avoid the problem that in main channel, amount of liquid reduces, amount of liquid reduces in reaction tank.2, multipurpose micro-fluidic chip of the present utility model, adopt the mode uniform distribution detected sample of centrifugal sample introduction, realize sample by the ratio of restriction main channel sectional area to distribute more uniformly, full up by liquid in reaction tank after the centrifugal distribution of the design of Buffer Pool guarantee, during whole reaction, in reaction tank, liquid remains full of, and stops product in each reaction tank to main channel and adjacent reaction pond internal diffusion.3, multipurpose micro-fluidic chip of the present utility model, by arranging waste liquid pool, has cushioned the sample feeding amount difference because user operation or sample injector difference cause, causes indivedual reaction tank discontented or excess liq is from problems such as steam vent spillings thus avoiding.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of embodiment of this utility model；
Fig. 2 (a)～(c) is the structural representation of three kinds of stepped holes of the present utility model；
Fig. 3 (a)～(d) is the structural representation of four kinds of liquid level blocking units of the present utility model；
Fig. 4 (a)～(d) is the structural representation of four kinds of waste liquid pools of the present utility model.
Detailed description of the invention
Below in conjunction with drawings and Examples, this utility model is described in detail.
As shown in Figure 1, the multipurpose micro-fluidic chip that this utility model provides, it includes an egative film 1 and and seals, with this egative film 1, the cover plate (not shown) coordinated, egative film 1 offers one or more corrugated main channel 2, main channel 2 is arranged in egative film 1 center for the circumference in the center of circle, each main channel 2 all includes multiple crest and trough, and crest is close to egative film 1 center position, trough all away from egative film 1 center position；The step-like sample holes 3 that one end of each main channel 2 is up big and down small with one is connected, the other end is connected with a steam vent 5 by a long capillary road 4, and sample holes 3, long capillary road 4 and steam vent 5 may be contained within egative film 1.The junction in each main channel 2 and long capillary road 4 is provided with a liquid level blocking unit 6, and the cross-sectional area of liquid level blocking unit 6 is more than the cross-sectional area being attached thereto logical main channel 2 and long capillary road 4.The trough of each main channel 2 connects pipeline 7 each through one and is connected with a reaction tank 8, in order to during rotary chip, the liquid in main channel 2 can be entered in reaction tank 8 by connection pipeline 7 under the influence of centrifugal force.Each connection pipeline 7 is provided with a Buffer Pool 9, and connection pipeline 7, reaction tank 8 and Buffer Pool 9 are also arranged on egative film 1.Structure except sample holes 3 and steam vent 5 on egative film 1 is then sealed by cover plate.
In above-described embodiment, the cross-sectional area at the crest place of main channel 2 is less than or equal to the cross-sectional area at trough place, and the ratio of the cross-sectional area of the cross-sectional area at the narrowest place of main channel 2 and the widest part is 0.2～1.
In above-described embodiment, sample holes 3 is the stepped hole being made up of a macropore 31 and an aperture 32, and the center superposition of the center of macropore 31 and aperture 32；The cross-sectional area of macropore 31 is 4～50 times of the cross-sectional area of aperture 32, and the degree of depth of macropore 31 is 40 μm～800 μm.If the diameter of macropore 31 is too little, the liquid remaining in sample injector fascia edge can drop to chip surface, and when operator's misalignment aperture 32, the liquid leaked outside also can spill into chip surface；If the diameter of macropore 31 is too big, in reacting by heating process the one side glue sealed membrane for sealing sample holes 3 bulge easily occurs, the phenomenon such as break.
In above-described embodiment, the aperture 32 of sample holes 3 is circular, and the size of aperture 32 just and in biotic experiment the size of conventional use of standard Tip head match, operator can use standard Tip head direct alignment apertures 32 sample introduction so that chip easily manufactures and facilitates operator to use.As shown in Fig. 2 (a)～(c), the shape of the macropore 31 of sample holes 3 can be circular, oval, square or other arbitrary shapes.
In above-described embodiment, it is preferable that the distance between steam vent 5 and sample holes 3 is not more than 10mm.Being arranged close to sample holes 3 by steam vent 5, distance is less between the two, it is possible to seals with an one side glue sealed membrane, does not result in the raw-material waste of sealed membrane, and simple to operation, and chip surface is more attractive.
In above-described embodiment, as shown in Fig. 3 (a)～(d), the shape of liquid level blocking unit 6 can be semicircle, oval, circular, square or other arbitrary shapes.Liquid level blocking unit 6 is possible to prevent the liquid in main channel 2 by surface tension traction to long capillary road 4, even overflow from steam vent 5, thus the amount of liquid avoided in the main channel after sample introduction 2 reduces, it also avoid the amount of liquid in the reaction tank 8 connected with main channel 2 to reduce, final guarantee testing result is effectively reliable.
In above-described embodiment, reaction tank 8 number is 5～100, and the volume of each reaction tank 8 is 0.1～25 μ L；Can be pre-loaded with in each reaction tank 8 can with the material of some composition generation specific reaction in testing sample or material or one section of nucleotide sequence；The volume of the main channel 2 of the peak-to-peak one section of V-arrangement of adjacent two ripples is 1.2～1.8 times of the volume of coupled logical reaction tank 8；The volume of Buffer Pool 9 is 0.2～0.8 times of the volume of reaction tank 8.
In above-described embodiment, as shown in Fig. 4 (a)～(d), the multiple reaction tanks 8 being connected with same main channel 2 are one group, be additionally provided with a waste liquid pool 10 between one section of main channel 2 and the liquid level blocking unit 6 corresponding with last reaction tank 8 of one group, the second thin pipe 12 that the first thin pipe 11 that waste liquid pool 10 includes being connected with main channel 2 is connected with liquid level blocking unit 6 and between the first thin pipe 11 and the second thin pipe 12 for carrying the waste liquid Buffer Pool 13 of appropriate amount of fluid；The volume of waste liquid pool 10 is not less than the volume of the main channel 2 corresponding with reaction tank 8；Waste liquid pool 10 can be designed to difformity according to demand.User operation difference or sample injector difference all can cause that sample liquid absorption has difference, when sample liquid absorption is very few, can cause no liquid sample in the part main channel 2 corresponding with from the reaction tank 8 close to liquid level blocking unit 6, ultimately result in corresponding reaction tank 8 and are discontented with；Otherwise, when sample liquid absorption is too much, can cause that liquid is from problems such as steam vent 5 spillings；And now waste liquid pool 10 can play the effect of buffering.
In above-described embodiment, the thickness of egative film 1 and cover plate is 0.05～5mm；Main channel 2, reaction tank 8, Buffer Pool 9 and the degree of depth connecting pipeline 7 are 40 μm～4mm.On egative film 1, the degree of depth of structure is subject to the impact of egative film 1 thickness, and suitable thickness can ensure that egative film 1 and cover plate are not easy deformation, and chip has enough loading amount of reagent, and the heat-conducting effect of material is unaffected, and chip internal is heated evenly, and does not affect testing result.
In above-described embodiment, as it is shown in figure 1, chip center is provided with central through hole 14, inward flange or chip outer edge at central through hole 14 are provided with at least one locating notch 15；Or, it is also possible to any suitable location place on chip arranges at least one hole, location.When centrifugal sample introduction, the effect of fixed chip is played in locating notch 15 or hole, location；When using instrument detection chip, locating notch 15 or hole, location are played fixed chip angle and determine the effect of sequence number of each reaction tank 8.
In above-described embodiment, micro-fluidic chip is additionally provided with sample partition identification 16, it is simple to operation identifies.
In above-described embodiment, egative film 1 adopts together with double faced adhesive tape (not shown) good bond with cover plate.The optical property of double faced adhesive tape is compatible with the detection means adopted, for example, when adopting through mode fluoroscopic examination, require that this double faced adhesive tape is had enough optical transmissions for fluorescing in reaction tank 8, when adopting reflective fluoroscopic examination, it is desirable to this double faced adhesive tape is of a sufficiently low at the fluorescence background of detected optical region.Double faced adhesive tape has enough bonding strengths, it is possible to various conventional heating conditions in tolerance bioanalysis, it is prevented that chip comes unglued leakage when being heated use, causes test crash or environmental pollution；Double faced adhesive tape also has suitable biocompatibility, it is possible to well keep biological activity and the chemical characteristic of testing sample and preloaded sample, the reaction that chip carries out will not be produced significant harmful effect.Adopt adhered by double sided plaster egative film 1 and cover plate, it is not necessary to use hot pressing and the expensive device of laser weld processing, less costly；Simultaneously, it is to avoid the harmful effect that hot pressing and laser weld process biological activity and chemical characteristic on being pre-loaded with sample on egative film 1 etc. produce, the impact that microfluidic channel shape is produced, even serious time the line clogging that causes or chip leakage.Certainly, egative film 1 and cover plate can also adopt the modes such as laser bonding to connect.
In above-described embodiment, after micro-fluidic chip sample introduction, adopt the one side glue sealed membrane with certain viscosity that sample holes 3 and steam vent 5 are sealed, whole reaction system is sealed in chip.
In above-described embodiment, micro-fluidic chip is when producing, the material for carrying out nucleic acid amplification reaction, biochemical reaction, immunoreation or the detection of other forms can be pre-loaded with as required respectively in different reaction tanks 8, or for the different material of same reaction formation detection, thus realize multiple application on a kind of chip platform.Such as, to on chip by the specific nucleic acid fragment (gene of such as certain particular person or the gene of pathogenic microorganism) in nucleic acid amplification reaction detection sample, it is possible to being pre-loaded with in differential responses pond can with the primer of different IPs acid fragment generation specific reaction in measuring samples and auxiliary element；To be detected the predetermined substance in sample or composition (such as blood glucose or triglyceride) on chip by biochemical reaction, it is possible to be pre-loaded with material and the auxiliary element that can react with different material in measuring samples or composition generation specific biochemical in differential responses pond；To detect the special component (such as certain antigen or antibody) in sample by immunoreation on chip, it is possible to being pre-loaded with in differential responses pond can with the material of different material in measuring samples or composition generation specific immune response and auxiliary element.
In use, first by sample injector from sample holes 3 to application of sample micro-fluidic chip, application of sample liquid fills main channel 2 and below liquid level blocking unit to this utility model；Due to the setting of liquid level blocking unit 6, application of sample liquid is intercepted by liquid level blocking unit 6, and the liquid in main channel 2 will not enter in long capillary road 4, overflows without from steam vent 5；When application of sample terminates, due to step-like sample holes 3, the liquid remaining in sample injector fascia edge is held by the macropore 31 of sample holes 3, no liquid water clock is to chip surface, when extracting sample injector, there will not be liquid drip leakage or splashes chip surface, absorbent paper wiping need not be used, simple to operation；After application of sample, chip is quiet to be put 10 seconds, liquid in the macropore 31 of sample holes 3 can capillary in main channel 2, only residual minim liquid in macropore 31, do not affect sample holes 3 and the sealing of steam vent 5, use one side glue sealed membrane that sample holes 3 and the steam vent 5 of chip are sealed.Micro-fluidic chip is placed on experimental facilities, centrifugal mode is adopted to be distributed to each reaction tank 8 by the detected sample in main channel 2, owing in main channel 2, amount of liquid is sufficient, and the setting of Buffer Pool 9 and waste liquid pool 10, there is the amount of liquid of abundance, it is ensured that the accuracy of test result in reaction tank 8；Simultaneously as only residual minim liquid in the macropore 31 of sample holes 3, in chip reacting by heating process, seal the sealed membrane of sample holes 3 and steam vent 5 without the phenomenon such as bulge, leakage.The methods such as fluorescence, turbidity or colour developing can be adopted, detected by instrument or the result of reaction in direct visual perception micro-fluidic chip；Can detect in real time in course of reaction, it is also possible to the reaction result on detection micro-fluidic chip after the completion of reaction.
The various embodiments described above are merely to illustrate this utility model; wherein each parts structure, position and connected mode etc. thereof are set all can be varied from; every equivalents carried out on the basis of technical solutions of the utility model and improvement, all should not get rid of outside protection domain of the present utility model.
Claims (10)
1. a multipurpose micro-fluidic chip, it is characterized in that, this chip includes an egative film and and seals, with described egative film, the cover plate coordinated, described egative film offers one or more corrugated main channel, described main channel is arranged in described plate center for the circumference in the center of circle, each described main channel all includes multiple crest and trough, and described crest is close to described plate center direction, described trough all away from described plate center direction；
The step-like sample holes that one end of each described main channel is up big and down small with one is connected, the other end is connected by a long capillary road and a steam vent, and described sample holes, long capillary road and steam vent may be contained within described egative film；
The junction in each described main channel and described long capillary road is provided with a liquid level blocking unit, and the cross-sectional area of described liquid level blocking unit is more than the cross-sectional area being attached thereto logical described main channel and long capillary road；
The trough of each described main channel connects pipeline each through one and a reaction tank is connected, and each described connection pipeline is provided with a Buffer Pool, and described connection pipeline, reaction tank and Buffer Pool are also arranged on described egative film；
Structure except described sample holes and steam vent on described egative film is sealed by described cover plate.
2. a kind of multipurpose micro-fluidic chip as claimed in claim 1, it is characterised in that the cross-sectional area at described crest place is 0.2～1 less than or equal to the ratio of the cross-sectional area at described trough place, the cross-sectional area at the narrowest place, described main channel and the cross-sectional area of the widest part.
3. a kind of multipurpose micro-fluidic chip as claimed in claim 1 or 2, it is characterised in that described sample holes is the stepped hole being made up of a macropore and an aperture, the center superposition of the center of described macropore and described aperture；The cross-sectional area of described macropore is 4～50 times of the cross-sectional area of described aperture, and the degree of depth of described macropore is 40 μm～800 μm；Described aperture is circular, and the size of described aperture matches with the size of conventional use of standard Tip head in biotic experiment；The shape of described macropore is circular, oval or square.
4. a kind of multipurpose micro-fluidic chip as claimed in claim 1 or 2, it is characterised in that the distance between described steam vent and described sample holes is not more than 10mm.
5. a kind of multipurpose micro-fluidic chip as claimed in claim 1 or 2, it is characterised in that the shape of described liquid level blocking unit is semicircle, oval, circular or square.
6. a kind of multipurpose micro-fluidic chip as claimed in claim 1 or 2, it is characterised in that the number of described reaction tank is 5～100, and the volume of each described reaction tank is 0.1～25 μ L；The volume of the peak-to-peak one section of V-arrangement main channel of ripple described in adjacent two is 1.2～1.8 times of the volume of coupled logical described reaction tank；The volume of described Buffer Pool is 0.2～0.8 times of the volume of described reaction tank.
7. a kind of multipurpose micro-fluidic chip as claimed in claim 6, it is characterized in that, the multiple described reaction tank being connected with main channel described in same is one group, be additionally provided with a waste liquid pool between one section of described main channel and the described liquid level blocking unit corresponding with last described reaction tank of one group, the second thin pipe that the first thin pipe that described waste liquid pool includes being connected with described main channel is connected with described liquid level blocking unit and between described first thin pipe and the second thin pipe for carrying the waste liquid Buffer Pool of liquid；The volume of described waste liquid pool is not less than the volume of one section of described V-arrangement main channel.
8. a kind of multipurpose micro-fluidic chip as described in claim 1 or 2 or 7, it is characterised in that the thickness of described egative film and cover plate is 0.05～5mm；The degree of depth of described main channel, reaction tank, Buffer Pool and connection pipeline is 40 μm～4mm.
9. a kind of multipurpose micro-fluidic chip as described in claim 1 or 2 or 7, it is characterised in that described chip center is provided with central through hole, is provided with at least one locating notch at the inward flange of described central through hole or the outer edge of described chip；Or, at least one hole, location it is provided with in the optional position of described chip；Described micro-fluidic chip is additionally provided with sample partition identification.
10. a kind of multipurpose micro-fluidic chip as described in claim 1 or 2 or 7, it is characterised in that be pre-loaded with the material for carrying out nucleic acid amplification reaction, biochemical reaction or immunoreation detection in different described reaction tanks respectively；Or, in different described reaction tanks, it is pre-loaded with the different material for same reaction detection respectively.
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| CN201620128203.3U CN205379906U (en) | 2016-02-19 | 2016-02-19 | Multipurpose micro -fluidic chip |
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| CN201620128203.3U CN205379906U (en) | 2016-02-19 | 2016-02-19 | Multipurpose micro -fluidic chip |
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