CN102845588A - Whey fermented feed and preparation method thereof - Google Patents
Whey fermented feed and preparation method thereof Download PDFInfo
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- CN102845588A CN102845588A CN2012103584405A CN201210358440A CN102845588A CN 102845588 A CN102845588 A CN 102845588A CN 2012103584405 A CN2012103584405 A CN 2012103584405A CN 201210358440 A CN201210358440 A CN 201210358440A CN 102845588 A CN102845588 A CN 102845588A
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Abstract
The invention provides a whey fermented feed. The whey fermented feed is characterized by comprising the following components by weight: 30%-40% of whey fermenting liquid, 20-30% of bean pulp, 10-20% of bran, 10-20% of brewer's grain, 3-8% of jujube powders, 2-5% of mountain flour, 0.2-0.5% of urea, and 0.5-1.5% of calcium hydrophosphate. A preparation method provided by the invention comprises the following steps: A. preparing whey fermenting liquid, B. preparing whey fermented feed, C. packaging, and D. fermenting. According to the invention, the process is simple, the method is effective, the waste is reduced, and the environmental pollution is avoided; the biological fermentation feed is prepared by the whey, the obtained fermented feed is fragrant, and excellent in palatability; a large amount of rich single-cell proteins, active lactobacillus, microelements and vitamins are generated after the fermentation; the biological active feed produced according to the method provided by the invention contains a plurality of nutrient substances such as rich single-cell proteins, active lactobacillus, microelements and vitamins, the cell number is greater than or equal to 2*1010 per gram, and the viable organism rate is greater than or equal to 85%.
Description
Technical field:
The present invention relates to the technique for producing feed field, particularly a kind of whey fermentation feed and preparation method thereof.
Background technology:
Whey is in the cheese production process, and milk condensing becomes isolated behind the ziega and is yellowish green, translucent liquid.Along with the development of new science and technology, whey has become the food and feeds raw material with high value and extensive use.Usually, the whey nutrient composition accounts for the 50-55% of milk nutritional labeling, and is nutritious, is rich in lactose in the whey, for microbial fermentation provides good C source.At present, medium and small Dairy Enterprise cheese byproduct-fresh whey and the direct discharging of molasses, how therefore the problem of initiation contaminated environment, utilize whey and molasses waste liquid to become technical barrier.
In view of above-mentioned Technology Need, simple in the urgent need to a kind of technique occurring, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, a kind of whey fermentation feed of the multiple nutrients materials such as single cell protein, biodiasmin, trace element and vitamin and preparation method thereof is enriched in generation in a large number after the fermentation.
Summary of the invention:
The object of the invention is to overcome the shortcoming that exists in the prior art, provide a kind of technique simple, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, a kind of whey fermentation feed of the multiple nutrients materials such as single cell protein, biodiasmin, trace element and vitamin and preparation method thereof is enriched in generation in a large number after the fermentation.
For achieving the above object, the invention discloses a kind of whey fermentation feed, wherein, dispose by weight following component:
Whey fermentation liquid 30%～40%, dregs of beans 20-30%, wheat bran 10-20%, brewex's grains 10-20%; Jujube powder 3-8%, stone flour 2-5%; Urea 0.2-0.5%; Calcium monohydrogen phosphate 0.5-1.5%.
Perhaps, dispose by weight following component:
Whey fermentation liquid 35%～40%, dregs of beans 25-30%, wheat bran 15-20%, brewex's grains 15-20%; Jujube powder 5-8%, stone flour 3-5%; Urea 0.3-0.5%; Calcium monohydrogen phosphate 1-1.5%.
Perhaps, dispose by weight following component:
Whey fermentation liquid 30%～35%, dregs of beans 20-25%, wheat bran 10-15%, brewex's grains 10-15%; Jujube powder 3-5%, stone flour 2-3%; Urea 0.2-0.3%; Calcium monohydrogen phosphate 0.5-1%.
Perhaps, dispose by weight following component:
Whey fermentation liquid 33%～36%, dregs of beans 23-28%, wheat bran 13-17%, brewex's grains 13-17%; Jujube powder 4-7%, stone flour 3-4%; Urea 0.3-0.4%; Calcium monohydrogen phosphate 0.8-1.2%.
Purpose to better implement the present invention, the present invention discloses again a kind of preparation method of whey fermentation feed, wherein, comprises the steps:
The step of A, preparation whey fermentation liquid;
The step of B, preparation whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then regulates pH value to 3.5～4.6 with citric acid solution, is making fermentation substrate behind sterilization 20～30min under 95 ℃ of conditions;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification is inoculated on the malt extract medium, cultivates 36h in 28 ℃;
The picking lactic acid bacteria culturers is cultivated 16～18h in 37 ℃ on the MRS culture medium;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and cultivates 6～10h under 28～30 ℃ of conditions;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80ml MRS fluid nutrient medium, cultivates 16～18h in 37 ℃;
The preparation of step 4, secondary seed solution:
The fermentation substrate of malt juice liquid medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, cultivates 18～20h under 28～30 ℃ of conditions;
The fermentation substrate of MRS fluid nutrient medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
With the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast that respectively step 4 is made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, filled into the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates;
With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions;
Step 6, prepare whey fermentation liquid in the workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, in three grades of seed liquor access fermentation tanks with two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and continues to cultivate 24h in 28～32 ℃, namely gets whey fermentation liquid.
In the described step 2, the barms of selecting is Candida or Kluyveromyces fragilis, and the lactobacillus inoculation of selecting is any one in Lactobacillus casei, Lactobacillus plantarum or the Pediococcus pentosaceus.
The B step is: with whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in the mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation was namely made whey fermentation feed of the present invention in 3-7 days.
Whey fermentation feed and preparation method thereof is in the application of field of fodder.
Advantage of the present invention is: technique is simple, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, single cell protein, biodiasmin, trace element and vitamin are enriched in generation in a large number after the fermentation, the bioactive feed that the inventive method is produced contains multiple nutrients materials such as enriching single cell protein, biodiasmin, trace element and vitamin, cell number 〉=2 * 1010/g, viable bacteria rate 〉=85%.
Description of drawings:
Fig. 1 is preparation method's flow chart of invention.
The specific embodiment:
Below in conjunction with accompanying drawing, invention is described.
As shown in Figure 1, Fig. 1 is preparation method's flow chart of invention.Preparation method of the present invention wherein, comprises the steps:
The step of A, preparation whey fermentation liquid;
The step of B, preparation whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then regulates pH value to 3.5～4.6 with citric acid solution, is making fermentation substrate behind sterilization 20～30min under 95 ℃ of conditions;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification is inoculated on the malt extract medium, cultivates 36h in 28 ℃;
The picking lactic acid bacteria culturers is cultivated 16～18h in 37 ℃ on the MRS culture medium;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and cultivates 6～10h under 28～30 ℃ of conditions;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80ml MRS fluid nutrient medium, cultivates 16～18h in 37 ℃;
The preparation of step 4, secondary seed solution:
The fermentation substrate of malt juice liquid medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, cultivates 18～20h under 28～30 ℃ of conditions;
The fermentation substrate of MRS fluid nutrient medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
With the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast that respectively step 4 is made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, filled into the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates;
With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions;
Step 6, prepare whey fermentation liquid in the workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, in three grades of seed liquor access fermentation tanks with two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and continues to cultivate 24h in 28～32 ℃, namely gets whey fermentation liquid.
In the step 2, the barms of selecting is Candida or Kluyveromyces fragilis, and the lactobacillus inoculation of selecting is any one in Lactobacillus casei, Lactobacillus plantarum or the Pediococcus pentosaceus.
The B step is: with whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in the mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation was namely made whey fermentation feed of the present invention in 3-7 days.
Weight ratio proportioning of the present invention is:
Whey fermentation liquid 30%～40%, dregs of beans 20-30%, wheat bran 10-20%, brewex's grains 10-20%; Jujube powder 3-8%, stone flour 2-5%; Urea 0.2-0.5%; Calcium monohydrogen phosphate 0.5-1.5%.
Perhaps, dispose by weight following component:
Whey fermentation liquid 35%～40%, dregs of beans 25-30%, wheat bran 15-20%, brewex's grains 15-20%; Jujube powder 5-8%, stone flour 3-5%; Urea 0.3-0.5%; Calcium monohydrogen phosphate 1-1.5%.
Perhaps, dispose by weight following component:
Whey fermentation liquid 30%～35%, dregs of beans 20-25%, wheat bran 10-15%, brewex's grains 10-15%; Jujube powder 3-5%, stone flour 2-3%; Urea 0.2-0.3%; Calcium monohydrogen phosphate 0.5-1%.
Perhaps, dispose by weight following component:
Whey fermentation liquid 33%～36%, dregs of beans 23-28%, wheat bran 13-17%, brewex's grains 13-17%; Jujube powder 4-7%, stone flour 3-4%; Urea 0.3-0.4%; Calcium monohydrogen phosphate 0.8-1.2%.
Example 1:
Fill a prescription according to the weight ratio configuration: whey fermentation liquid 30%; Dregs of beans 30%; Wheat bran 20%; Brewex's grains 14%; Jujube powder 3%, stone flour 2.3%; Urea 0.2%; Calcium monohydrogen phosphate 0.5%.
The present embodiment preparation method wherein, comprises the steps:
The step of A, preparation whey fermentation liquid;
The step of B, preparation whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then regulates pH value to 3.5～4.6 with citric acid solution, is making fermentation substrate behind sterilization 20～30min under 95 ℃ of conditions;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Candida is inoculated on the malt extract medium, cultivates 36h in 28 ℃;
Picking lactic acid bacteria culturers-Lactobacillus casei is cultivated 16～18h in 37 ℃ on the MRS culture medium;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and cultivates 6～10h under 28～30 ℃ of conditions;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80ml MRS fluid nutrient medium, cultivates 16～18h in 37 ℃;
The preparation of step 4, secondary seed solution:
The fermentation substrate of malt juice liquid medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, cultivates 18～20h under 28～30 ℃ of conditions;
The fermentation substrate of MRS fluid nutrient medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, and the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, in 3
Cultivate 10～12h under 3～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
With the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast that respectively step 4 is made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, filled into the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates;
With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions;
Step 6, prepare whey fermentation liquid in the workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, in three grades of seed liquor access fermentation tanks with two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and continues to cultivate 24h in 28～32 ℃, namely gets whey fermentation liquid.
The B step is: with whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in the mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation was namely made whey fermentation feed of the present invention in 3-7 days.
Example 2:
Fill a prescription according to the weight ratio configuration: whey fermentation liquid 40%; Dregs of beans 20%; Wheat bran 10%; Brewex's grains 15%; Jujube powder 8%, stone flour 5%; Urea 0.5%; Calcium monohydrogen phosphate 1.5%.
The present embodiment preparation method wherein, comprises the steps:
The step of A, preparation whey fermentation liquid;
The step of B, preparation whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then regulates pH value to 3.5～4.6 with citric acid solution, is making fermentation substrate behind sterilization 20～30min under 95 ℃ of conditions;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Kluyveromyces fragilis is inoculated on the malt extract medium, cultivates 36h in 28 ℃;
Picking lactic acid bacteria culturers-Lactobacillus plantarum is cultivated 16～18h in 37 ℃ on the MRS culture medium;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and cultivates 6～10h under 28～30 ℃ of conditions;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80ml MRS fluid nutrient medium, cultivates 16～18h in 37 ℃;
The preparation of step 4, secondary seed solution:
The fermentation substrate of malt juice liquid medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, cultivates 18～20h under 28～30 ℃ of conditions;
The fermentation substrate of MRS fluid nutrient medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
With the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast that respectively step 4 is made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, filled into the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates;
With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions;
Step 6, prepare whey fermentation liquid in the workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, in three grades of seed liquor access fermentation tanks with two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and continues to cultivate 24h in 28～32 ℃, namely gets whey fermentation liquid.
The B step is: with whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in the mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation was namely made whey fermentation feed of the present invention in 3-7 days.
Example 3:
Prescription: whey fermentation liquid 35%; Dregs of beans 25%; Wheat bran 20%; Brewex's grains 10%; Jujube powder 5%, stone flour 3.6%; Urea 0.4%; Calcium monohydrogen phosphate 1.0%.
The present embodiment preparation method wherein, comprises the steps:
The step of A, preparation whey fermentation liquid;
The step of B, preparation whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then regulates pH value to 3.5～4.6 with citric acid solution, is making fermentation substrate behind sterilization 20～30min under 95 ℃ of conditions;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Kluyveromyces fragilis is inoculated on the malt extract medium, cultivates 36h in 28 ℃;
Picking lactic acid bacteria culturers-Pediococcus pentosaceus is cultivated 16～18h in 37 ℃ on the MRS culture medium;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and cultivates 6～10h under 28～30 ℃ of conditions;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80ml MRS fluid nutrient medium, cultivates 16～18h in 37 ℃;
The preparation of step 4, secondary seed solution:
The fermentation substrate of malt juice liquid medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, cultivates 18～20h under 28～30 ℃ of conditions;
The fermentation substrate of MRS fluid nutrient medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
With the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast that respectively step 4 is made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, filled into the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates;
With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions;
Step 6, prepare whey fermentation liquid in the workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, in three grades of seed liquor access fermentation tanks with two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and continues to cultivate 24h in 28～32 ℃, namely gets whey fermentation liquid.
The B step is: with whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in the mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation was namely made whey fermentation feed of the present invention in 3-7 days.
Technique of the present invention is simple, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, single cell protein, biodiasmin, trace element and vitamin are enriched in generation in a large number after the fermentation, the bioactive feed that the inventive method is produced contains multiple nutrients materials such as enriching single cell protein, biodiasmin, trace element and vitamin, cell number 〉=2 * 1010/g, viable bacteria rate 〉=85%.
Claims (10)
1. a whey fermentation feed is characterized in that, disposes by weight following component:
Whey fermentation liquid 30%-40%, dregs of beans 20-30%, wheat bran 10-20%, brewex's grains 10-20%; Jujube powder 3-8%, stone flour 2-5%; Urea 0.2-0.5%; Calcium monohydrogen phosphate 0.5-1.5%.
2. described whey fermentation feed according to claim 1 is characterized in that, disposes by weight following component:
Whey fermentation liquid 35%-40%, dregs of beans 25-30%, wheat bran 15-20%, brewex's grains 15-20%; Jujube powder 5-8%, stone flour 3-5%; Urea 0.3-0.5%; Calcium monohydrogen phosphate 1-1.5%.
3. described whey fermentation feed according to claim 1 is characterized in that, disposes by weight following component:
Whey fermentation liquid 30%-35%, dregs of beans 20-25%, wheat bran 10-15%, brewex's grains 10-15%; Jujube powder 3-5%, stone flour 2-3%; Urea 0.2-0.3%; Calcium monohydrogen phosphate 0.5-1%.
4. described whey fermentation feed according to claim 1 is characterized in that, disposes by weight following component:
Whey fermentation liquid 33%～36%, dregs of beans 23-28%, wheat bran 13-17%, brewex's grains 13-17%; Jujube powder 4-7%, stone flour 3-4%; Urea 0.3-0.4%; Calcium monohydrogen phosphate 0.8-1.2%.
One kind arbitrary such as claim 1-4 as described in the preparation method of whey fermentation feed, it is characterized in that, comprise the steps:
The step of A, preparation whey fermentation liquid;
The step of B, preparation whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
6. the preparation method of described whey fermentation feed according to claim 5 is characterized in that the A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then regulates pH value to 3.5～4.6 with citric acid solution, is making fermentation substrate behind sterilization 20～30min under 95 ℃ of conditions;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification is inoculated on the malt extract medium, cultivates 36h in 28 ℃;
The picking lactic acid bacteria culturers is cultivated 16～18h in 37 ℃ on the MRS culture medium;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and cultivates 6～10h under 28～30 ℃ of conditions;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on the 80ml MRS fluid nutrient medium, cultivates 16～18h in 37 ℃;
The preparation of step 4, secondary seed solution:
The fermentation substrate of malt juice liquid medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, cultivates 18～20h under 28～30 ℃ of conditions;
The fermentation substrate of MRS fluid nutrient medium with step 1 preparation mixed in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, cultivates 10～12h under 33～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
With the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast that respectively step 4 is made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, filled into the fermentation substrate 20L of step 1 preparation in per 4 hours, 15min per hour ventilates;
With the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, cultivates 24h under 33～37 ℃ of conditions;
Step 6, prepare whey fermentation liquid in the workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, in three grades of seed liquor access fermentation tanks with two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and continues to cultivate 24h in 28～32 ℃, namely gets whey fermentation liquid.
7. the preparation method of described whey fermentation feed according to claim 6, it is characterized in that, in the described step 2, the barms of selecting is Candida or Kluyveromyces fragilis, and the lactobacillus inoculation of selecting is any one in Lactobacillus casei, Lactobacillus plantarum or the Pediococcus pentosaceus.
8. the preparation method of described whey fermentation feed according to claim 5 is characterized in that the B step is: with whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in the mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
9. the preparation method of described whey fermentation feed according to claim 5 is characterized in that the D step is: the breathing bag standing for fermentation was namely made whey fermentation feed of the present invention in 3-7 days.
One kind arbitrary such as claim 1-9 as described in whey fermentation feed and preparation method thereof in the application of field of fodder.
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