CN102845588B - Whey fermented feed and preparation method thereof - Google Patents
Whey fermented feed and preparation method thereof Download PDFInfo
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- CN102845588B CN102845588B CN201210358440.5A CN201210358440A CN102845588B CN 102845588 B CN102845588 B CN 102845588B CN 201210358440 A CN201210358440 A CN 201210358440A CN 102845588 B CN102845588 B CN 102845588B
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- 239000005862 Whey Substances 0.000 title claims abstract description 85
- 102000007544 Whey Proteins Human genes 0.000 title claims abstract description 85
- 108010046377 Whey Proteins Proteins 0.000 title claims abstract description 85
- 238000002360 preparation method Methods 0.000 title claims abstract description 69
- 238000000855 fermentation Methods 0.000 claims abstract description 132
- 230000004151 fermentation Effects 0.000 claims abstract description 132
- 239000007788 liquid Substances 0.000 claims abstract description 64
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 19
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 19
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000004202 carbamide Substances 0.000 claims abstract description 19
- 235000013339 cereals Nutrition 0.000 claims abstract description 19
- 235000013312 flour Nutrition 0.000 claims abstract description 19
- 239000000843 powder Substances 0.000 claims abstract description 19
- 235000015097 nutrients Nutrition 0.000 claims abstract description 18
- 240000008866 Ziziphus nummularia Species 0.000 claims abstract 2
- 238000010899 nucleation Methods 0.000 claims description 60
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 48
- 239000000758 substrate Substances 0.000 claims description 42
- 239000002609 medium Substances 0.000 claims description 31
- 239000002054 inoculum Substances 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 26
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 24
- 239000004310 lactic acid Substances 0.000 claims description 24
- 235000014655 lactic acid Nutrition 0.000 claims description 24
- 238000012856 packing Methods 0.000 claims description 24
- 230000001580 bacterial effect Effects 0.000 claims description 18
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 18
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 18
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 18
- 239000004575 stone Substances 0.000 claims description 18
- 235000015099 wheat brans Nutrition 0.000 claims description 18
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 12
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 12
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 235000013379 molasses Nutrition 0.000 claims description 8
- 230000004913 activation Effects 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 229940017800 lactobacillus casei Drugs 0.000 claims description 4
- 244000126002 Ziziphus vulgaris Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 14
- 108010027322 single cell proteins Proteins 0.000 abstract description 8
- 235000013343 vitamin Nutrition 0.000 abstract description 8
- 239000011782 vitamin Substances 0.000 abstract description 8
- 229940088594 vitamin Drugs 0.000 abstract description 8
- 229930003231 vitamin Natural products 0.000 abstract description 8
- 239000002699 waste material Substances 0.000 abstract description 6
- 235000019629 palatability Nutrition 0.000 abstract description 5
- 241000186660 Lactobacillus Species 0.000 abstract description 4
- 229940039696 lactobacillus Drugs 0.000 abstract description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 241001247821 Ziziphus Species 0.000 description 16
- 239000011573 trace mineral Substances 0.000 description 6
- 235000013619 trace mineral Nutrition 0.000 description 6
- 150000003722 vitamin derivatives Chemical class 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- 229940072205 lactobacillus plantarum Drugs 0.000 description 3
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- 244000285963 Kluyveromyces fragilis Species 0.000 description 2
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 2
- 244000199866 Lactobacillus casei Species 0.000 description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 description 2
- 240000006024 Lactobacillus plantarum Species 0.000 description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 2
- 241000191996 Pediococcus pentosaceus Species 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- -1 biodiasmin Substances 0.000 description 2
- 235000013351 cheese Nutrition 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a whey fermented feed. The whey fermented feed is characterized by comprising the following components by weight: 30%-40% of whey fermenting liquid, 20-30% of bean pulp, 10-20% of bran, 10-20% of brewer's grain, 3-8% of jujube powders, 2-5% of mountain flour, 0.2-0.5% of urea, and 0.5-1.5% of calcium hydrophosphate. A preparation method provided by the invention comprises the following steps: A. preparing whey fermenting liquid, B. preparing whey fermented feed, C. packaging, and D. fermenting. According to the invention, the process is simple, the method is effective, the waste is reduced, and the environmental pollution is avoided; the biological fermentation feed is prepared by the whey, the obtained fermented feed is fragrant, and excellent in palatability; a large amount of rich single-cell proteins, active lactobacillus, microelements and vitamins are generated after the fermentation; the biological active feed produced according to the method provided by the invention contains a plurality of nutrient substances such as rich single-cell proteins, active lactobacillus, microelements and vitamins, the cell number is greater than or equal to 2*1010 per gram, and the viable organism rate is greater than or equal to 85%.
Description
Technical field:
The present invention relates to the technique for producing feed field, particularly a kind of whey fermentation feed and preparation method thereof.
background technology:
Whey is in the cheese production process, and milk condensing becomes isolated after ziega and is yellowish green, translucent liquid.Along with the development of new science and technology, whey has become the food and feeds raw material with high value and extensive use.Typically, the whey nutrient composition accounts for the 50-55% of milk nutritional labeling, nutritious, is rich in lactose in whey, for the microorganism fermentation provides good C source.At present, medium and small Dairy Enterprise cheese byproduct-fresh whey and the direct discharge of molasses, how the problem of initiation contaminated environment, therefore, utilize whey and molasses waste liquid to become technical barrier.
In view of above-mentioned Technology Need, simple in the urgent need to a kind of technique occurring, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, after fermentation, a kind of whey fermentation feed of the multiple nutrients materials such as single cell protein, biodiasmin, trace element and vitamin and preparation method thereof is enriched in generation in a large number.
summary of the invention:
The object of the invention is to overcome the shortcoming existed in prior art, provide a kind of technique simple, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, after fermentation, a kind of whey fermentation feed of the multiple nutrients materials such as single cell protein, biodiasmin, trace element and vitamin and preparation method thereof is enriched in generation in a large number.
For achieving the above object, the invention discloses a kind of whey fermentation feed, wherein, configure by weight following component:
Whey fermentation liquid 30%～40%, dregs of beans 20-30%, wheat bran 10-20%, brewex's grains 10-20%; Jujube powder 3-8%, stone flour 2-5%; Urea 0.2-0.5%; Calcium monohydrogen phosphate 0.5-1.5%.
Perhaps, configure by weight following component:
Whey fermentation liquid 35%～40%, dregs of beans 25-30%, wheat bran 15-20%, brewex's grains 15-20%; Jujube powder 5-8%, stone flour 3-5%; Urea 0.3-0.5%; Calcium monohydrogen phosphate 1-1.5%.
Perhaps, configure by weight following component:
Whey fermentation liquid 30%～35%, dregs of beans 20-25%, wheat bran 10-15%, brewex's grains 10-15%; Jujube powder 3-5%, stone flour 2-3%; Urea 0.2-0.3%; Calcium monohydrogen phosphate 0.5-1%.
Perhaps, configure by weight following component:
Whey fermentation liquid 33%～36%, dregs of beans 23-28%, wheat bran 13-17%, brewex's grains 13-17%; Jujube powder 4-7%, stone flour 3-4%; Urea 0.3-0.4%; Calcium monohydrogen phosphate 0.8-1.2%.
Purpose to better implement the present invention, the present invention discloses again a kind of preparation method of whey fermentation feed, wherein, comprises the steps:
A, prepare the step of whey fermentation liquid;
B, prepare the step of whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then with citric acid solution, regulates pH value to 3.5～4.6, under 95 ℃ of conditions, after sterilization 20～30min, makes fermentation substrate;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification, be inoculated on malt extract medium, in 28 ℃, cultivates 36h;
The picking lactic acid bacteria culturers, on the MRS culture medium, is cultivated 16～18h in 37 ℃;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and under 28～30 ℃ of conditions, cultivates 6～10h;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on 80ml MRS fluid nutrient medium, in 37 ℃, cultivates 16～18h;
The preparation of step 4, secondary seed solution:
Malt juice liquid medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, under 28～30 ℃ of conditions, cultivate 18～20h;
The MRS fluid nutrient medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, under 33～37 ℃ of conditions, cultivate 10～12h;
The preparation of step 5, three grades of seed liquor:
By the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast respectively step 4 made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, within every 4 hours, fill into the fermentation substrate 20L of step 1 preparation, 15min per hour ventilates;
By the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, under 33～37 ℃ of conditions, cultivates 24h;
Step 6, prepare whey fermentation liquid in workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, by three grades of seed liquor access fermentation tanks of two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and in 28～32 ℃, continues to cultivate 24h, obtains whey fermentation liquid.
In described step 2, the barms of selecting is Candida or Kluyveromyces fragilis, and the lactobacillus inoculation of selecting is any one in Lactobacillus casei, Lactobacillus plantarum or Pediococcus pentosaceus.
The B step is: by whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation is made to whey fermentation feed of the present invention in 3-7 days.
Whey fermentation feed and preparation method thereof is in the application of field of fodder.
Advantage of the present invention is: technique is simple, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, after fermentation, single cell protein, biodiasmin, trace element and vitamin are enriched in generation in a large number, the bioactive feed that the inventive method is produced contains multiple nutrients materials such as enriching single cell protein, biodiasmin, trace element and vitamin, cell number >=2 * 1010/g, viable bacteria rate >=85%.
The accompanying drawing explanation:
Fig. 1 is preparation method's flow chart of invention.
The specific embodiment:
Below in conjunction with accompanying drawing, invention is described.
As shown in Figure 1, Fig. 1 is preparation method's flow chart of invention.Preparation method of the present invention, wherein, comprise the steps:
A, prepare the step of whey fermentation liquid;
B, prepare the step of whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then with citric acid solution, regulates pH value to 3.5～4.6, under 95 ℃ of conditions, after sterilization 20～30min, makes fermentation substrate;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification, be inoculated on malt extract medium, in 28 ℃, cultivates 36h;
The picking lactic acid bacteria culturers, on the MRS culture medium, is cultivated 16～18h in 37 ℃;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and under 28～30 ℃ of conditions, cultivates 6～10h;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on 80ml MRS fluid nutrient medium, in 37 ℃, cultivates 16～18h;
The preparation of step 4, secondary seed solution:
Malt juice liquid medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, under 28～30 ℃ of conditions, cultivate 18～20h;
The MRS fluid nutrient medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, under 33～37 ℃ of conditions, cultivate 10～12h;
The preparation of step 5, three grades of seed liquor:
By the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast respectively step 4 made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, within every 4 hours, fill into the fermentation substrate 20L of step 1 preparation, 15min per hour ventilates;
By the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, under 33～37 ℃ of conditions, cultivates 24h;
Step 6, prepare whey fermentation liquid in workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, by three grades of seed liquor access fermentation tanks of two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and in 28～32 ℃, continues to cultivate 24h, obtains whey fermentation liquid.
In step 2, the barms of selecting is Candida or Kluyveromyces fragilis, and the lactobacillus inoculation of selecting is any one in Lactobacillus casei, Lactobacillus plantarum or Pediococcus pentosaceus.
The B step is: by whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation is made to whey fermentation feed of the present invention in 3-7 days.
Weight ratio proportioning of the present invention is:
Whey fermentation liquid 30%～40%, dregs of beans 20-30%, wheat bran 10-20%, brewex's grains 10-20%; Jujube powder 3-8%, stone flour 2-5%; Urea 0.2-0.5%; Calcium monohydrogen phosphate 0.5-1.5%.
Perhaps, configure by weight following component:
Whey fermentation liquid 35%～40%, dregs of beans 25-30%, wheat bran 15-20%, brewex's grains 15-20%; Jujube powder 5-8%, stone flour 3-5%; Urea 0.3-0.5%; Calcium monohydrogen phosphate 1-1.5%.
Perhaps, configure by weight following component:
Whey fermentation liquid 30%～35%, dregs of beans 20-25%, wheat bran 10-15%, brewex's grains 10-15%; Jujube powder 3-5%, stone flour 2-3%; Urea 0.2-0.3%; Calcium monohydrogen phosphate 0.5-1%.
Perhaps, configure by weight following component:
Whey fermentation liquid 33%～36%, dregs of beans 23-28%, wheat bran 13-17%, brewex's grains 13-17%; Jujube powder 4-7%, stone flour 3-4%; Urea 0.3-0.4%; Calcium monohydrogen phosphate 0.8-1.2%.
Example 1:
According to the weight ratio configuration, fill a prescription: whey fermentation liquid 30%; Dregs of beans 30%; Wheat bran 20%; Brewex's grains 14%; Jujube powder 3%, stone flour 2.3%; Urea 0.2%; Calcium monohydrogen phosphate 0.5%.
The present embodiment preparation method, wherein, comprise the steps:
A, prepare the step of whey fermentation liquid;
B, prepare the step of whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then with citric acid solution, regulates pH value to 3.5～4.6, under 95 ℃ of conditions, after sterilization 20～30min, makes fermentation substrate;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Candida, be inoculated on malt extract medium, in 28 ℃, cultivates 36h;
Picking lactic acid bacteria culturers-Lactobacillus casei, on the MRS culture medium, is cultivated 16～18h in 37 ℃;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and under 28～30 ℃ of conditions, cultivates 6～10h;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on 80ml MRS fluid nutrient medium, in 37 ℃, cultivates 16～18h;
The preparation of step 4, secondary seed solution:
Malt juice liquid medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, under 28～30 ℃ of conditions, cultivate 18～20h;
The MRS fluid nutrient medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, and the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, in 3
Cultivate 10～12h under 3～37 ℃ of conditions;
The preparation of step 5, three grades of seed liquor:
By the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast respectively step 4 made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, within every 4 hours, fill into the fermentation substrate 20L of step 1 preparation, 15min per hour ventilates;
By the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, under 33～37 ℃ of conditions, cultivates 24h;
Step 6, prepare whey fermentation liquid in workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, by three grades of seed liquor access fermentation tanks of two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and in 28～32 ℃, continues to cultivate 24h, obtains whey fermentation liquid.
The B step is: by whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation is made to whey fermentation feed of the present invention in 3-7 days.
Example 2:
According to the weight ratio configuration, fill a prescription: whey fermentation liquid 40%; Dregs of beans 20%; Wheat bran 10%; Brewex's grains 15%; Jujube powder 8%, stone flour 5%; Urea 0.5%; Calcium monohydrogen phosphate 1.5%.
The present embodiment preparation method, wherein, comprise the steps:
A, prepare the step of whey fermentation liquid;
B, prepare the step of whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then with citric acid solution, regulates pH value to 3.5～4.6, under 95 ℃ of conditions, after sterilization 20～30min, makes fermentation substrate;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Kluyveromyces fragilis, be inoculated on malt extract medium, in 28 ℃, cultivates 36h;
Picking lactic acid bacteria culturers-Lactobacillus plantarum, on the MRS culture medium, is cultivated 16～18h in 37 ℃;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and under 28～30 ℃ of conditions, cultivates 6～10h;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on 80ml MRS fluid nutrient medium, in 37 ℃, cultivates 16～18h;
The preparation of step 4, secondary seed solution:
Malt juice liquid medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, under 28～30 ℃ of conditions, cultivate 18～20h;
The MRS fluid nutrient medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, under 33～37 ℃ of conditions, cultivate 10～12h;
The preparation of step 5, three grades of seed liquor:
By the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast respectively step 4 made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, within every 4 hours, fill into the fermentation substrate 20L of step 1 preparation, 15min per hour ventilates;
By the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, under 33～37 ℃ of conditions, cultivates 24h;
Step 6, prepare whey fermentation liquid in workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, by three grades of seed liquor access fermentation tanks of two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and in 28～32 ℃, continues to cultivate 24h, obtains whey fermentation liquid.
The B step is: by whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation is made to whey fermentation feed of the present invention in 3-7 days.
Example 3:
Formula: whey fermentation liquid 35%; Dregs of beans 25%; Wheat bran 20%; Brewex's grains 10%; Jujube powder 5%, stone flour 3.6%; Urea 0.4%; Calcium monohydrogen phosphate 1.0%.
The present embodiment preparation method, wherein, comprise the steps:
A, prepare the step of whey fermentation liquid;
B, prepare the step of whey fermentation feed;
The step of C, packing;
The step of D, fermentation.
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then with citric acid solution, regulates pH value to 3.5～4.6, under 95 ℃ of conditions, after sterilization 20～30min, makes fermentation substrate;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Kluyveromyces fragilis, be inoculated on malt extract medium, in 28 ℃, cultivates 36h;
Picking lactic acid bacteria culturers-Pediococcus pentosaceus, on the MRS culture medium, is cultivated 16～18h in 37 ℃;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and under 28～30 ℃ of conditions, cultivates 6～10h;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on 80ml MRS fluid nutrient medium, in 37 ℃, cultivates 16～18h;
The preparation of step 4, secondary seed solution:
Malt juice liquid medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, under 28～30 ℃ of conditions, cultivate 18～20h;
The MRS fluid nutrient medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, under 33～37 ℃ of conditions, cultivate 10～12h;
The preparation of step 5, three grades of seed liquor:
By the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast respectively step 4 made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, within every 4 hours, fill into the fermentation substrate 20L of step 1 preparation, 15min per hour ventilates;
By the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, under 33～37 ℃ of conditions, cultivates 24h;
Step 6, prepare whey fermentation liquid in workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, by three grades of seed liquor access fermentation tanks of two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and in 28～32 ℃, continues to cultivate 24h, obtains whey fermentation liquid.
The B step is: by whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in mixer and is stirred; The C step is: B step products obtained therefrom is packed with breathing bag.
The D step is: the breathing bag standing for fermentation is made to whey fermentation feed of the present invention in 3-7 days.
Technique of the present invention is simple, method is effective, cut the waste, avoid contaminated environment, utilize whey to prepare biological fermentation feed, gained fermented feed fragrant odour, good palatability, after fermentation, single cell protein, biodiasmin, trace element and vitamin are enriched in generation in a large number, the bioactive feed that the inventive method is produced contains multiple nutrients materials such as enriching single cell protein, biodiasmin, trace element and vitamin, cell number >=2 * 1010/g, viable bacteria rate >=85%.
Claims (1)
1. the preparation method of a whey fermentation feed, is characterized in that, comprises the steps:
According to the weight ratio configuration, fill a prescription: whey fermentation liquid 30%; Dregs of beans 30%; Wheat bran 20%; Brewex's grains 14%; Jujube powder 3%, stone flour 2.3%; Urea 0.2%; Calcium monohydrogen phosphate 0.5%;
Operation in accordance with the following steps:
A, prepare the step of whey fermentation liquid;
B, prepare the step of whey fermentation feed;
The step of C, packing;
The step of D, fermentation;
The A step further comprises the steps:
The preparation of step 1, fermentation substrate:
Fresh whey is mixed with molasses, and quality is 2: 1 than ratio, then with citric acid solution, regulates pH value to 3.5～4.6, under 95 ℃ of conditions, after sterilization 20～30min, makes fermentation substrate;
The activation of step 2, bacterial classification:
Picking fodder yeast bacterial classification-Candida, be inoculated on malt extract medium, in 28 ℃, cultivates 36h;
Picking lactic acid bacteria culturers-Lactobacillus casei, on the MRS culture medium, is cultivated 16～18h in 37 ℃;
The preparation of step 3, primary seed solution:
The fodder yeast activated spawn that step 2 is made all is inoculated in the conical flask that the 80ml malt juice liquid medium is housed, and under 28～30 ℃ of conditions, cultivates 6～10h;
The lactic acid bacteria activated spawn that step 2 is made all is inoculated into and is equipped with on 80ml MRS fluid nutrient medium, in 37 ℃, cultivates 16～18h;
The preparation of step 4, secondary seed solution:
Malt juice liquid medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the fodder yeast primary seed solution that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 4%, under 28～30 ℃ of conditions, cultivate 18～20h;
The MRS fluid nutrient medium is mixed with the fermentation substrate of step 1 preparation in 2: 1 by volume, pack in the seeding tank of 5L, charge weight is 60%～70% of seeding tank volume, the primary seed solution of the lactic acid bacteria that step 3 is made is inoculated into the seeding tank of 5L by inoculum concentration 5%～10%, under 33～37 ℃ of conditions, cultivate 10～12h;
The preparation of step 5, three grades of seed liquor:
By the fermentation substrate of the step 1 preparation 500L seeding tank of packing into, liquid amount is 50%, in the secondary seed solution access 500L seeding tank of the fodder yeast respectively step 4 made, inoculum concentration is 20～25L, in 28～32 ℃ stir after, continue to cultivate 24h, within every 4 hours, fill into the fermentation substrate 20L of step 1 preparation, 15min per hour ventilates;
By the fermentation substrate of the step 1 preparation 1000L seeding tank of packing into, liquid amount is 60%～70%, and in the secondary seed solution access 1000L seeding tank of the lactic acid bacteria that step 4 is made, inoculum concentration is 50L, under 33～37 ℃ of conditions, cultivates 24h;
Step 6, prepare whey fermentation liquid in workshop:
The fresh whey sterile working 2000L fermentation tank of packing into, by three grades of seed liquor access fermentation tanks of two kinds of bacterial classifications, inoculum concentration is 15～20L and stirs, and in 28～32 ℃, continues to cultivate 24h, obtains whey fermentation liquid;
The B step is: by whey fermentation liquid; Dregs of beans; Wheat bran; Brewex's grains; The jujube powder, stone flour; Urea; Calcium monohydrogen phosphate is put in mixer and is stirred;
The C step is: B step products obtained therefrom is packed with breathing bag;
The D step is: by the breathing bag standing for fermentation, within 3-7 days, be the obtain whey fermented feed.
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| CN109480060A (en) * | 2018-12-19 | 2019-03-19 | 陕西兴荣昌农业科技股份有限公司 | A kind of pannage and its preparation method and application prepared using fermentation red date powder |
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