CN109007254A - A kind of preparation method of fermented feed - Google Patents

A kind of preparation method of fermented feed Download PDF

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Publication number
CN109007254A
CN109007254A CN201810847892.7A CN201810847892A CN109007254A CN 109007254 A CN109007254 A CN 109007254A CN 201810847892 A CN201810847892 A CN 201810847892A CN 109007254 A CN109007254 A CN 109007254A
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Prior art keywords
whey
fermentation
seeding tank
seed solution
liquid
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CN201810847892.7A
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Chinese (zh)
Inventor
文发祥
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Puding County Agricultural Development Co Ltd
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Puding County Agricultural Development Co Ltd
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Priority to CN201810847892.7A priority Critical patent/CN109007254A/en
Publication of CN109007254A publication Critical patent/CN109007254A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Sustainable Development (AREA)
  • Inorganic Chemistry (AREA)
  • Fodder In General (AREA)

Abstract

The present invention relates to a kind of ecosystem fermented feed and preparation method thereof, component parts are as follows: corn flour, rape cake, fish meal, conch meal, bone meal, probiotics, whey fermentation liquid and calcium monohydrogen phosphate.Present invention process is simple, and method is effective, reduces waste, avoid pollution environment, biological fermentation feed is prepared using whey, gained fermented feed fragrant odour, palatability are good, and largely abundant single cell protein, biodiasmin, microelement and vitamin are generated after fermentation.

Description

A kind of preparation method of fermented feed
Technical field
The present invention relates to technique for producing feed field, in particular to a kind of technique for producing fermented feed.
Background technique
Whey is in cheese production process, and milk condensing is in yellow green, translucent at isolating after ziega Liquid.With the development of new science and technology, whey is had become with high value and widely applied food and feedstuff.Usually and Speech, whey nutrient ingredient accounts for about the 50-55% of milk nutritional ingredient, full of nutrition, and lactose is rich in whey, sends out for microorganism Ferment provides the good source C.Currently, the direct emission of the fresh whey and molasses of medium and small Dairy Enterprise cheese byproduct-, causes Therefore how the problem of polluting environment utilizes whey and molasses waste liquid to become technical problem.And corn flour, rape cake, shell Powder, bone meal, beneficial natural resources probiotics etc. are common feed ingredients, can enrich the mouthfeel of feed, have additional nutrients ingredient.But these It is all common ingredient, how the utilization of high quality also needs further Study on Transformation.
It is needed in view of above-mentioned technology, there is an urgent need to a kind of simple process occur, method is effective, reduces waste, avoids pollution Environment prepares biological fermentation feed using whey, and gained fermented feed fragrant odour, palatability are good, generates after fermentation a large amount of rich A kind of work for producing fermented feed of a variety of nutriments such as rich single cell protein, biodiasmin, microelement and vitamin Skill.
Summary of the invention
It is an object of the invention to overcome disadvantage existing in the prior art, a kind of simple process is provided, method is effective, subtracts Little waste avoids pollution environment, prepares biological fermentation feed using whey, and gained fermented feed fragrant odour, palatability are good, hair A kind of life of a variety of nutriments such as a large amount of abundant single cell proteins, biodiasmin, microelement and vitamin is generated after ferment The technique for producing fermented feed.
To achieve the above object, the invention discloses a kind of preparation methods of fermented feed, which is characterized in that including as follows Step:
A, whey fermentation liquid is prepared；B, whey fermentation feed is prepared；C, it packs: step B products obtained therefrom breathing bag is close Package dress；D, it ferments: breathing bag being left to ferment 3-7 days and obtains whey fermentation feed of the present invention.
Wherein, step A further comprises:
A1, fermentation substrate: fresh whey being mixed with molasses, ratio 2:1, then with citric acid solution adjust pH value to 3.5~4.6, fermentation substrate is made after 20~30min is sterilized under the conditions of 95 DEG C；
A2, primary seed solution: the candida strain after activation is inoculated into the triangle equipped with malt juice liquid medium In flask, 6~10h is cultivated under the conditions of 28~30 DEG C, obtains yeast primary seed solution；By the cheese cream bar after the activation after activation Bacterium strain is inoculated into equipped on MRS fluid nutrient medium, in 37 DEG C of 16~18h of culture, obtains lactobacillus primary seed solution；
A3, secondary seed solution:
By malt juice liquid medium, 2:1 is mixed by volume with fermentation substrate obtained, is fitted into the seeding tank of 5L, dress Enter 60%~70% that amount is seed tank volume, yeast primary seed solution is inoculated into the seeding tank of 5L by inoculum concentration 4%, in 28- 20h is cultivated under the conditions of 30 DEG C；
By MRS fluid nutrient medium, 2:1 is mixed by volume with fermentation substrate, is fitted into the seeding tank of 5L, and charge weight is kind Lactic acid bacteria primary seed solution is inoculated into the seeding tank of 5L, in 33-37 by the 60%~70% of sub- tank volume by inoculum concentration 5-10% 12h is cultivated under the conditions of DEG C；
A4, three-level seed liquor:
Yeast secondary seed solution loading amount is the 10% of seeding tank, is 50% fermentation substrate in 28-32 DEG C of continuation with liquid amount Culture for 24 hours, fills into the 10% of fermentation substrate seeding tank in every 4 hours；
Lactic acid bacteria secondary seed solution loading amount is the 5% of seeding tank, is 65% fermentation substrate in 33-37 DEG C of condition with liquid amount Lower culture is for 24 hours；
A5, whey fermentation liquid:
Fresh whey sterile working is packed into 2000L fermentor, by the three-level seed liquor access fermentor of two kinds of strains, connects Kind amount is that 15-20L is stirred evenly, and continues culture in 28-32 DEG C for 24 hours to get whey fermentation liquid.
Wherein, step B further comprises: corn flour, rape cake, fish meal, conch meal, bone meal, fish meal, beneficial natural resources are prebiotic Bacterium, whey fermentation liquid, calcium monohydrogen phosphate are put into blender and are stirred evenly.
The formula of step B configures following components by weight:
Whey fermentation liquid 30-40%, corn flour 10%-20%, rape cake 20%-30%, fish meal 5-8%, conch meal 2%-5%, bone meal 3%-8%, beneficial natural resources probiotics 10%-20%, calcium monohydrogen phosphate 0.5%-1.5%.
Advantages of the present invention are as follows: simple process, method is effective, reduces waste, avoids pollution environment, is prepared and is given birth to using whey Object fermented feed, gained fermented feed fragrant odour, palatability are good, and largely abundant single cell protein, Lactic acid bacteria drink are generated after fermentation The bioactive feed of sour bacterium, microelement and vitamin, the method for the present invention production contains abundant single cell protein, active lactic acid A variety of nutriments such as bacterium, microelement and vitamin, cell number >=2 × 1010A/g, viable bacteria rate >=85%.
Specific embodiment:
Embodiment 1
A kind of preparation method of fermented feed, which comprises the steps of:
A, whey fermentation liquid is prepared；B, whey fermentation feed is prepared；C, it packs: step B products obtained therefrom breathing bag is close Package dress；D, it ferments: breathing bag is left to ferment 3 days up to whey fermentation feed.
Wherein, step A further comprises:
A1, fermentation substrate: fresh whey being mixed with molasses, ratio 2:1, then with citric acid solution adjust pH value to 3.5~4.6, fermentation substrate is made after 20~30min is sterilized under the conditions of 95 DEG C；
A2, primary seed solution: the candida strain after activation is inoculated into the triangle equipped with malt juice liquid medium In flask, 6~10h is cultivated under the conditions of 28~30 DEG C, obtains yeast primary seed solution；By the cheese cream bar after the activation after activation Bacterium strain is inoculated into equipped on MRS fluid nutrient medium, in 37 DEG C of 16~18h of culture, obtains lactobacillus primary seed solution；
A3, secondary seed solution:
By malt juice liquid medium, 2:1 is mixed by volume with fermentation substrate obtained, is fitted into the seeding tank of 5L, dress Enter 60%~70% that amount is seed tank volume, yeast primary seed solution is inoculated into the seeding tank of 5L by inoculum concentration 4%, in 28- 20h is cultivated under the conditions of 30 DEG C；
By MRS fluid nutrient medium, 2:1 is mixed by volume with fermentation substrate, is fitted into the seeding tank of 5L, and charge weight is kind Lactic acid bacteria primary seed solution is inoculated into the seeding tank of 5L, in 33-37 by the 60%~70% of sub- tank volume by inoculum concentration 5-10% 12h is cultivated under the conditions of DEG C；
A4, three-level seed liquor:
Yeast secondary seed solution loading amount is the 10% of seeding tank, is 50% fermentation substrate in 28-32 DEG C of continuation with liquid amount Culture for 24 hours, fills into the 10% of fermentation substrate seeding tank in every 4 hours；
Lactic acid bacteria secondary seed solution loading amount be seeding tank 5%, with liquid amount be 65% fermentation substrate in
It is cultivated for 24 hours under the conditions of 33-37 DEG C；
A5, whey fermentation liquid:
Fresh whey sterile working is packed into 2000L fermentor, by the three-level seed liquor access fermentor of two kinds of strains, connects Kind amount is that 15-20L is stirred evenly, and continues culture in 28-32 DEG C for 24 hours to get whey fermentation liquid.
Wherein, step B further comprises: corn flour, rape cake, fish meal, conch meal, bone meal, fish meal, beneficial natural resources are prebiotic Bacterium, whey fermentation liquid, calcium monohydrogen phosphate are put into blender and are stirred evenly.
The formula of step B configures following components by weight:
Whey fermentation liquid 40%, corn flour 10%, rape cake 30%, fish meal 5%, conch meal 5%, bone meal 3%, beneficial natural resources Probiotics 20%, calcium monohydrogen phosphate 0.5%.
Embodiment 2
A kind of preparation method of fermented feed, which comprises the steps of:
A, whey fermentation liquid is prepared；B, whey fermentation feed is prepared；C, it packs: step B products obtained therefrom breathing bag is close Package dress；D, it ferments: breathing bag being left to ferment 3-7 days and obtains whey fermentation feed of the present invention.
Wherein, step A further comprises:
A1, fermentation substrate: fresh whey being mixed with molasses, ratio 2:1, then with citric acid solution adjust pH value to 3.5~4.6, fermentation substrate is made after 20~30min is sterilized under the conditions of 95 DEG C；
A2, primary seed solution: the candida strain after activation is inoculated into the triangle equipped with malt juice liquid medium In flask, 6~10h is cultivated under the conditions of 28~30 DEG C, obtains yeast primary seed solution；By the cheese cream bar after the activation after activation Bacterium strain is inoculated into equipped on MRS fluid nutrient medium, in 37 DEG C of 16~18h of culture, obtains lactobacillus primary seed solution；
A3, secondary seed solution:
By malt juice liquid medium, 2:1 is mixed by volume with fermentation substrate obtained, is fitted into the seeding tank of 5L, dress Enter 60%~70% that amount is seed tank volume, yeast primary seed solution is inoculated into the seeding tank of 5L by inoculum concentration 4%, in 28- 20h is cultivated under the conditions of 30 DEG C；
By MRS fluid nutrient medium, 2:1 is mixed by volume with fermentation substrate, is fitted into the seeding tank of 5L, and charge weight is kind Lactic acid bacteria primary seed solution is inoculated into the seeding tank of 5L, in 33-37 by the 60%~70% of sub- tank volume by inoculum concentration 5-10% 12h is cultivated under the conditions of DEG C；
A4, three-level seed liquor:
Yeast secondary seed solution loading amount is the 10% of seeding tank, is 50% fermentation substrate in 28-32 DEG C of continuation with liquid amount Culture for 24 hours, fills into the 10% of fermentation substrate seeding tank in every 4 hours；
Lactic acid bacteria secondary seed solution loading amount is the 5% of seeding tank, is 65% fermentation substrate in 33-37 DEG C of condition with liquid amount Lower culture is for 24 hours；
A5, whey fermentation liquid:
Fresh whey sterile working is packed into 2000L fermentor, by the three-level seed liquor access fermentor of two kinds of strains, connects Kind amount is that 15-20L is stirred evenly, and continues culture in 28-32 DEG C for 24 hours to get whey fermentation liquid.
Wherein, step B further comprises: corn flour, rape cake, fish meal, conch meal, bone meal, fish meal, beneficial natural resources are prebiotic Bacterium, whey fermentation liquid, calcium monohydrogen phosphate are put into blender and are stirred evenly.
The formula of step B configures following components by weight:
Whey fermentation liquid 30%, corn flour 20%, rape cake 20%, fish meal 8%, conch meal 2%, bone meal 8%, beneficial natural resources Probiotics 10%, calcium monohydrogen phosphate 1.5%.

Claims (4)

1. a kind of preparation method of fermented feed, which comprises the steps of:
A, whey fermentation liquid is prepared；B, whey fermentation feed is prepared；C, it packs: step B products obtained therefrom being sealed with breathing bag and is wrapped Dress；D, it ferments: breathing bag being left to ferment 3-7 days and obtains whey fermentation feed of the present invention.
2. the preparation method of fermented feed as described in claim 1, which is characterized in that step A further comprises:
A1, fermentation substrate: fresh whey being mixed with molasses, ratio 2:1, then adjusts pH value to 3.5 with citric acid solution ~4.6, fermentation substrate is made after 20~30min is sterilized under the conditions of 95 DEG C；
A2, primary seed solution: the candida strain after activation is inoculated into the conical flask equipped with malt juice liquid medium In, 6~10h is cultivated under the conditions of 28~30 DEG C, obtains yeast primary seed solution；By the Lactobacillus casei bacterium after the activation after activation Kind is inoculated into equipped on MRS fluid nutrient medium, in 37 DEG C of 16~18h of culture, obtains lactobacillus primary seed solution；
A3, secondary seed solution:
By malt juice liquid medium, 2:1 is mixed by volume with fermentation substrate obtained, is fitted into the seeding tank of 5L, charge weight It is the 60%~70% of seed tank volume, yeast primary seed solution is inoculated into the seeding tank of 5L by inoculum concentration 4%, in 28-30 DEG C Under the conditions of cultivate 20h；
By MRS fluid nutrient medium, 2:1 is mixed by volume with fermentation substrate, is fitted into the seeding tank of 5L, charge weight is seeding tank Lactic acid bacteria primary seed solution is inoculated into the seeding tank of 5L by the 60%~70% of volume by inoculum concentration 5-10%, in 33-37 DEG C of item 12h is cultivated under part；
A4, three-level seed liquor:
Yeast secondary seed solution loading amount is the 10% of seeding tank, continues to cultivate in 28-32 DEG C for 50% fermentation substrate with liquid amount For 24 hours, the 10% of fermentation substrate seeding tank is filled within every 4 hours；
Lactic acid bacteria secondary seed solution loading amount is the 5% of seeding tank, is that 65% fermentation substrate is trained under the conditions of 33-37 DEG C with liquid amount It supports for 24 hours；
A5, whey fermentation liquid:
Fresh whey sterile working is packed into 2000L fermentor, the three-level seed liquor of two kinds of strains is accessed in fermentor, inoculum concentration It is that 15-20L is stirred evenly, continues culture in 28-32 DEG C for 24 hours to get whey fermentation liquid.
3. the preparation method of fermented feed as described in claim 1, which is characterized in that step B further comprises: by corn Powder, rape cake, fish meal, conch meal, bone meal, beneficial natural resources probiotics, whey fermentation liquid, calcium monohydrogen phosphate put into stirring in blender Uniformly.
4. the preparation method of fermented feed as claimed in claim 3, which is characterized in that the formula of step B is by weight configuration Following components:
Whey fermentation liquid 30-40%, corn flour 10%-20%, rape cake 20%-30%, fish meal 5-8%, conch meal 2%- 5%, bone meal 3%-8%, beneficial natural resources probiotics 10%-20%, calcium monohydrogen phosphate 0.5%-1.5%.
CN201810847892.7A 2018-07-27 2018-07-27 A kind of preparation method of fermented feed Pending CN109007254A (en)

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Application Number Priority Date Filing Date Title
CN201810847892.7A CN109007254A (en) 2018-07-27 2018-07-27 A kind of preparation method of fermented feed

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Application Number Priority Date Filing Date Title
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120041503A (en) * 2010-10-21 2012-05-02 순천대학교 산학협력단 The liquid fermented feed using the whey and skim milk powder and preparation method thereof
CN102845588A (en) * 2012-09-25 2013-01-02 济南亚奥生物技术有限公司 Whey fermented feed and preparation method thereof
CN102972622A (en) * 2012-10-12 2013-03-20 青岛田瑞生态科技有限公司 Preparation method of whey fermented forage

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120041503A (en) * 2010-10-21 2012-05-02 순천대학교 산학협력단 The liquid fermented feed using the whey and skim milk powder and preparation method thereof
CN102845588A (en) * 2012-09-25 2013-01-02 济南亚奥生物技术有限公司 Whey fermented feed and preparation method thereof
CN102972622A (en) * 2012-10-12 2013-03-20 青岛田瑞生态科技有限公司 Preparation method of whey fermented forage

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Application publication date: 20181218