CN102816751B - 一种高活性壳聚糖酶及其制备方法 - Google Patents
一种高活性壳聚糖酶及其制备方法 Download PDFInfo
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- CN102816751B CN102816751B CN201210300876.9A CN201210300876A CN102816751B CN 102816751 B CN102816751 B CN 102816751B CN 201210300876 A CN201210300876 A CN 201210300876A CN 102816751 B CN102816751 B CN 102816751B
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- 238000000034 method Methods 0.000 claims abstract description 23
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- BIGPRXCJEDHCLP-UHFFFAOYSA-N ammonium bisulfate Chemical compound [NH4+].OS([O-])(=O)=O BIGPRXCJEDHCLP-UHFFFAOYSA-N 0.000 claims description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 3
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 claims description 3
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- 238000004064 recycling Methods 0.000 claims description 3
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- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 claims 2
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- 241000894006 Bacteria Species 0.000 description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 7
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- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 6
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 230000006196 deacetylation Effects 0.000 description 3
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- 229920002101 Chitin Polymers 0.000 description 2
- KIWBPDUYBMNFTB-UHFFFAOYSA-N Ethyl hydrogen sulfate Chemical compound CCOS(O)(=O)=O KIWBPDUYBMNFTB-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
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- 229910019142 PO4 Inorganic materials 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
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CN102816751B true CN102816751B (zh) | 2014-05-21 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105801675B (zh) * | 2016-03-15 | 2019-02-15 | 黄河三角洲京博化工研究院有限公司 | 一种高活性壳聚糖酶控制基因csn及利用该基因生产高活性壳聚糖酶的方法 |
CN108531418B (zh) * | 2018-01-25 | 2021-05-28 | 山东省农业科学院农产品研究所 | 一种壳聚糖酶的制备方法 |
CN108441440B (zh) * | 2018-01-25 | 2021-03-23 | 山东省农业科学院农产品研究所 | 一种蜡状芽孢杆菌116及其应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1492039A (zh) * | 2003-06-21 | 2004-04-28 | 北海国发海洋生物产业股份有限公司 | 壳聚糖酶生产菌及低聚壳聚糖的生产方法 |
CN101148646A (zh) * | 2007-09-06 | 2008-03-26 | 武汉东方天琪生物工程有限公司 | 一种高活力壳聚糖酶制剂的生产方法 |
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2012
- 2012-08-22 CN CN201210300876.9A patent/CN102816751B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1492039A (zh) * | 2003-06-21 | 2004-04-28 | 北海国发海洋生物产业股份有限公司 | 壳聚糖酶生产菌及低聚壳聚糖的生产方法 |
CN101148646A (zh) * | 2007-09-06 | 2008-03-26 | 武汉东方天琪生物工程有限公司 | 一种高活力壳聚糖酶制剂的生产方法 |
Non-Patent Citations (4)
Title |
---|
Gao XA等.Purification and characterization of chitosanase from Bacillus cereus D-11.《Carbohydrate Polymers》.2008,第72卷513-520. |
Purification and characterization of chitosanase from Bacillus cereus D-11;Gao XA等;《Carbohydrate Polymers》;20081231;第72卷;513-520 * |
王平平等.细菌产壳聚糖酶的性质初探.《上海水产大学学报》.2003,第12卷(第4期),380-383. |
细菌产壳聚糖酶的性质初探;王平平等;《上海水产大学学报》;20031231;第12卷(第4期);380-383 * |
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