CN102746703A - Production method for extracting active substances from beer lees - Google Patents
Production method for extracting active substances from beer lees Download PDFInfo
- Publication number
- CN102746703A CN102746703A CN201110100026XA CN201110100026A CN102746703A CN 102746703 A CN102746703 A CN 102746703A CN 201110100026X A CN201110100026X A CN 201110100026XA CN 201110100026 A CN201110100026 A CN 201110100026A CN 102746703 A CN102746703 A CN 102746703A
- Authority
- CN
- China
- Prior art keywords
- lees
- ethanol
- sodium hydroxide
- brewer
- grains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Belonging to the technical field of food processing, the invention provides a production method for extracting active substances from beer lees. The method comprises: 1. adding ethanol into distiller dried lees, with the lees and the ethanol being in a ratio of 1:3-1:10, controlling an ultrasonic time at 50-120min, and performing centrifugation at a speed of 5000r/min so as to obtain a pigment of the beer lees; 2. adding ethanol and sodium hydroxide solutions into the remaining solid of the lees, with ethanol and the sodium hydroxide being in a ratio of 1:1-1:5, and controlling the lees and the ethanol-sodium hydroxide solution in a ratio of 1:40-1:80, conducting extraction for 60-120min, performing centrifugation at a speed of 5000r/min, adjusting the pH to 4.7, carrying out centrifugation at a speed of 5000r/min, thus obtaining protein of the beer lees; and 3. adding a sodium hydroxide solution into the remaining solid of the lees, controlling the solid of the lees and the sodium hydroxide solution in a ratio of 1:15-1:20, conducting heating to a temperature of 50-80DEG C, then performing stirring and extraction, carrying out centrifugation at a speed of 5000r/min, thus obtaining the dietary fiber of the beer lees. The method of the invention has the beneficial effects that: (1) integrated comprehensive utilization of beer lees is realized, specifically from pigment extraction, protein separation, dietary fiber, and finally to feed application; and (2) the method is characterized by environmental protection and energy saving, joint application of combined techniques, and mild technological conditions.
Description
Technical field
The invention belongs to food processing technology field.
Background technology
In recent years, China's brewing industry development has become the big beer prodn of the first in the world state rapidly, year production beer 7 * 107t, and the brewer's grains of generation can reach 10 * 106t.But China's beer enterprise is with its direct discharging to the processing majority of brewer's grains, and peasant's producing fodder is given in the undersell that has.Brewer's grains contain a large amount of food fibres and protein isoreactivity material, are a kind of good dietary fiber sources and protein source.How to turn waste into wealth, rationally utilize brewer's grains, make it to produce economic benefit, solve problem of environmental pollution and wasting of resources problem effectively, become the key subjects that brewing industry faces.
Document and patent also have the report of some brewer's grains deep processings; But to its utilize mode comparatively single, have plenty of and after simple process, process animal-feed, as with hydrolysis Enzymatic Extraction protein; After just obtaining protein merely; Abandoned other activeconstituentss, utilization ratio and economic worth are all lower, and a lot of active substances are all wasted.Wheat is poor to produce food fibre, feed oil, protein powder simultaneously for utilizing of having, but method is complicated, and extraction yield is not high, and the vinasse residue after the extraction is further utilization not.
Summary of the invention
The objective of the invention is: a kind of working method of from brewer's grains, extracting active substance is provided; It can extract the pigment in the brewer's grains, protein, food fibre successively, does not influence the active and application of pigment, protein, food fibre in the whole leaching process.
Method of the present invention is:
1, get water ratio 5% following distiller's dried grain, adding concentration is the ethanol of 70%-100%, control vinasse and proportion of ethanol (weight: be 1 volume): 3-1: 10; Extract the brewer's grains pigment with the ultrasonic disruption appearance, ultrasonic time is 50-120 minute; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got supernatant; Rotary evaporation obtains the brewer's grains pigment.
2, take by weighing the residue vinasse solid that extracts behind the pigment; Add ethanol and sodium hydroxide solution; Ethanol (concentration is more than 95%) and sodium hydroxide (concentration is 0.3-1%) are than being 1: 1-1: 5 (volume ratios); Control vinasse solid and ethanol sodium hydroxide solution ratio be (weight: volume) 1: 40-1: 80, extraction time is 60-120 minute; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got supernatant; Transfer pH4.7; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got deposition; Lyophilize obtains protein in the brewer's grains.
3, take by weighing the residue vinasse solid that extracts behind pigment and the protein; Adding concentration is the 0.5%-2% sodium hydroxide solution; Control vinasse solid and alkaline solution ratio be (weight: volume) 1: 15-1: 20, water-bath is heated to 50-80 ℃ of following stirring and leaching, extraction time 60-100 minute; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got supernatant; Rotary evaporation obtains the brewer's grains food fibre.
Residue vinasse solid behind first three extracting section intact pigment, protein and the food fibre is processed feed, main through dry, pulverize, mix after adding additive, then packing.
The invention has the beneficial effects as follows: the brewer's grains comprehensive utilization is integrated under (1) environmental protection and energy saving background.Satisfying under the background of environmental protection and energy saving; From pigment extraction, albumen sepn, food fibre, arrive feed applications at last, overall design brewer's grains comprehensive utilization flow process; And be core technology with the food fibre extractive technique with proteins extraction technology; Extract protein through ultrasonic extraction method pigment, pure alkaline process, and then extract the food fibre in the brewer's grains, at last remaining vinasse slag is processed into animal-feed with alkaline process.(2) environmental protection and energy saving, the combination technique combined utilization, processing condition are gentle.In the whole brewer's grains comprehensive utilization process; Adopt and do not destroy active substance and stable extractive technique thereof in the brewer's grains; Particularly use the combination of multiple processing mode and use, make that activeconstituents can farthest be extracted and utilize in the brewer's grains, and reach the requirement of environmental protection and energy saving.
Description of drawings
Fig. 1 is production process route figure of the present invention.
Embodiment
Embodiment 1:
Wet vinasse through dry, pulverize after, accurately take by weighing vinasse powder double centner, be 450 liters of 95% ethanolic solns by 1: 4.5 ratio of solid-to-liquid ratio adding concentration; Be placed in the supersonic cell crusher and extract pigment; 70 minutes extraction times, 744.00 kilowatts of ultrasonic powers, centrifugal 4000 commentaries on classics/PMs; The supernatant concentrating under reduced pressure gets 3.5 kilograms of pigments.Lower sediment was by 1: 50 (Kg: l) add pure alkali (1: 3) solution and extract protein, stir 90 minutes extraction times; Centrifugal 15 minutes (4000 commentaries on classics/PM) behind the supernatant accent pH4.7 centrifugal 12 minutes (5000 commentaries on classics/PM), gets 10.2 kilograms in protein; Lower sediment is extracted food fibre with alkaline process again; By 1: 17 (Kg: l) add (0.9%) 150 liter in sodium hydroxide, 70 ℃ of water bath heating temperatures, 80 minutes extraction times of solid-to-liquid ratio.Extract the back spinning and obtain two portions, first part is a supernatant, and adjust pH is 4.7, recentrifuge.Supernatant is neutralized to neutral back rotary evaporation, concentrating liquid, then with long-pending 95% ethanol sedimentation of its tetraploid, leave standstill the regular hour after, filter, drying, 10.5 kilograms of water-soluble dietary fibres.Residue vinasse system feed is subsequent use.
Claims (1)
1. working method of from brewer's grains, extracting active substance, its method is:
A, get water ratio 5% following distiller's dried grain, adding concentration is the ethanol of 70%-100%, and control vinasse and ethanol are 1 by weight the ratio of volume: 3-1: 10; Extract the brewer's grains pigment with the ultrasonic disruption appearance, ultrasonic time is 50-120 minute; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got supernatant; Rotary evaporation obtains the brewer's grains pigment;
B, take by weighing the residue vinasse solid that extracts behind the pigment; Add ethanol and sodium hydroxide solution; Concentration is that the sodium hydroxide volume ratio that ethanol and the concentration more than 95% is 0.3-1% is 1: 1-1: 5; Control vinasse solid and ethanol sodium hydroxide solution are 1 by weight volume ratio: 40-1: 80, and extraction time is 60-120 minute; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got supernatant; Transfer pH4.7; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got deposition; Lyophilize obtains protein in the brewer's grains;
C, take by weighing the residue vinasse solid that extracts behind pigment and the protein; Adding concentration is the 0.5%-2% sodium hydroxide solution; Control vinasse solid and alkaline solution are 1 by weight volume ratio: 15-1: 20, and water-bath is heated to 50-80 ℃ of following stirring and leaching, extraction time 60-100 minute; Centrifugal 5000 commentaries on classics/PMs more than 10 minutes, are got supernatant; Rotary evaporation obtains the brewer's grains food fibre.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110100026XA CN102746703A (en) | 2011-04-21 | 2011-04-21 | Production method for extracting active substances from beer lees |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110100026XA CN102746703A (en) | 2011-04-21 | 2011-04-21 | Production method for extracting active substances from beer lees |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102746703A true CN102746703A (en) | 2012-10-24 |
Family
ID=47027214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110100026XA Pending CN102746703A (en) | 2011-04-21 | 2011-04-21 | Production method for extracting active substances from beer lees |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102746703A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960742A (en) * | 2012-12-17 | 2013-03-13 | 遵义师范学院 | Method for extracting water-soluble dietary fiber in vinasse by using enzyme method |
| CN105361168A (en) * | 2015-12-02 | 2016-03-02 | 绥化学院 | Composite antioxidation peptides and preparation method thereof |
| CN105728431A (en) * | 2016-02-24 | 2016-07-06 | 齐芳 | Cleaning technology for vinasse |
| CN108812910A (en) * | 2018-06-22 | 2018-11-16 | 东北林业大学 | Vinasse albumen compounds beverage and preparation method thereof |
| CN115518131A (en) * | 2022-10-11 | 2022-12-27 | 青海互助天佑德青稞酒股份有限公司 | Medicinal composition containing highland barley vinasse extract and preparation method thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2155042A1 (en) * | 1994-08-04 | 1996-02-05 | Sohtaroh Kishi | Process for the preparation of protein-rich product from brewer's spent grain |
| JPH08157385A (en) * | 1994-12-07 | 1996-06-18 | Kirin Brewery Co Ltd | Bowl movement improver, agent for enhancing prolifration of gut mucosa and perpectal nutrition promoting agent |
| JPH08205808A (en) * | 1995-02-01 | 1996-08-13 | Kozaburo Shiba | Effective utilization of ume vinegar, soy strained lee, beer strained lee and bean curd refuse as industrial waste |
| JP2005065580A (en) * | 2003-08-25 | 2005-03-17 | Keiichi Ozawa | Method for producing agaricus blazei murill mycelium, and method and apparatus for producing extract from the resultant mycelium |
| JP2005143480A (en) * | 2003-11-17 | 2005-06-09 | Hasegawa Hidetoshi | Method for producing industrial waste such as rice bran, soybean-curd refuse, beer lees and the like |
| CN1765226A (en) * | 2005-11-21 | 2006-05-03 | 中国食品发酵工业研究院 | Method for producing diet fiber, feedstuff oil and protein powder simultaneously by use of wheat grains |
| CN101073402A (en) * | 2006-05-15 | 2007-11-21 | 上海承润生物科技发展有限公司 | Method for production soy-sauce with beer yeast and distiller's grain as raw materials and special compound enzyme |
| CN101935717A (en) * | 2010-08-23 | 2011-01-05 | 吉林大学 | Method for efficiently separating xylose from beer lees |
-
2011
- 2011-04-21 CN CN201110100026XA patent/CN102746703A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2155042A1 (en) * | 1994-08-04 | 1996-02-05 | Sohtaroh Kishi | Process for the preparation of protein-rich product from brewer's spent grain |
| JPH08157385A (en) * | 1994-12-07 | 1996-06-18 | Kirin Brewery Co Ltd | Bowl movement improver, agent for enhancing prolifration of gut mucosa and perpectal nutrition promoting agent |
| JPH08205808A (en) * | 1995-02-01 | 1996-08-13 | Kozaburo Shiba | Effective utilization of ume vinegar, soy strained lee, beer strained lee and bean curd refuse as industrial waste |
| JP2005065580A (en) * | 2003-08-25 | 2005-03-17 | Keiichi Ozawa | Method for producing agaricus blazei murill mycelium, and method and apparatus for producing extract from the resultant mycelium |
| JP2005143480A (en) * | 2003-11-17 | 2005-06-09 | Hasegawa Hidetoshi | Method for producing industrial waste such as rice bran, soybean-curd refuse, beer lees and the like |
| CN1765226A (en) * | 2005-11-21 | 2006-05-03 | 中国食品发酵工业研究院 | Method for producing diet fiber, feedstuff oil and protein powder simultaneously by use of wheat grains |
| CN101073402A (en) * | 2006-05-15 | 2007-11-21 | 上海承润生物科技发展有限公司 | Method for production soy-sauce with beer yeast and distiller's grain as raw materials and special compound enzyme |
| CN101935717A (en) * | 2010-08-23 | 2011-01-05 | 吉林大学 | Method for efficiently separating xylose from beer lees |
Non-Patent Citations (2)
| Title |
|---|
| 姜福佳: "酒糟中活性成分的提取及其活性的研究", 《吉林大学硕士学位论文》 * |
| 王异静等: "从啤酒糟中提取水溶性膳食纤维的研究", 《酿酒》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960742A (en) * | 2012-12-17 | 2013-03-13 | 遵义师范学院 | Method for extracting water-soluble dietary fiber in vinasse by using enzyme method |
| CN105361168A (en) * | 2015-12-02 | 2016-03-02 | 绥化学院 | Composite antioxidation peptides and preparation method thereof |
| CN105728431A (en) * | 2016-02-24 | 2016-07-06 | 齐芳 | Cleaning technology for vinasse |
| CN108812910A (en) * | 2018-06-22 | 2018-11-16 | 东北林业大学 | Vinasse albumen compounds beverage and preparation method thereof |
| CN108812910B (en) * | 2018-06-22 | 2022-01-28 | 东北林业大学 | Vinasse protein compound beverage and preparation method thereof |
| CN115518131A (en) * | 2022-10-11 | 2022-12-27 | 青海互助天佑德青稞酒股份有限公司 | Medicinal composition containing highland barley vinasse extract and preparation method thereof |
| CN115518131B (en) * | 2022-10-11 | 2023-06-20 | 青海互助天佑德青稞酒股份有限公司 | Medicinal composition containing highland barley distillers' grains extract and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102746703A (en) | Production method for extracting active substances from beer lees | |
| CN104211827B (en) | A kind of method extracting polysaccharide from Hericium erinaceus (Bull. Ex Fr.) Pers. dreg | |
| CN100469256C (en) | Method for preparing yeast peptide utilizing beer yeast | |
| CN107362810A (en) | A kind of magnetic mao bamboon charcoal solid acid catalyst and preparation method and its application in xylo-oligosaccharide is prepared | |
| CN102453614A (en) | New method for comprehensively utilizing food wastes | |
| CN104000138A (en) | Process method for preparing biological protein-rich dietary fiber and pectin simultaneously by taking potato residues as raw materials | |
| CN104611381A (en) | Method for producing ethanol by continuous enzymolysis and fermentation of lignocellulose | |
| CN101775417A (en) | Method for extracting anthocyanin from blueberry pomace | |
| CN103864945B (en) | A kind of method strengthening polysaccharide in extraction Cordyceps militaris rice medium | |
| CN102994209B (en) | Method for synchronously extracting soybean oil and soybean protein through aqueous enzymatic method | |
| CN101423855A (en) | Method for preparing polysaccharide by using lucidum strain fermented laminaria leftover | |
| CN102295984A (en) | Method and device for producing fruit oil of cornus wilsoniana | |
| WO2016173262A1 (en) | Method for producing cellulosic ethanol by fermenting agricultural and forest biomass waste thick mash | |
| CN102887732A (en) | Method for producing amino acid liquid fertilizer by processing wastewater via tilapias | |
| CN104072627A (en) | Preparation method for typhonium giganteum polysaccharide extract | |
| CN107937446A (en) | A kind of technique using maize straw as waste ethanol | |
| CN101213994A (en) | Method for using ultrasound wave auxiliary enzyme to extract apple seed oil | |
| CN103923949A (en) | Method and apparatus for producing ethanol through synchronous saccharification and fermentation of lignocellulose | |
| CN106011181A (en) | Method for preparation of fuel ethanol from kitchen garbage | |
| CN103636917A (en) | Method for extracting rice protein from rice residue by using hydraulic cavitation technology | |
| CN102746366B (en) | Method for extracting tea saponin from camellia oleifera cake | |
| CN102220381B (en) | Technology for producing edible alcohol by using crushed vermicelli and bean dregs | |
| CN102807629A (en) | Method for extracting rice bran polysaccharides by using continuous countercurrent ultrasonic equipment | |
| CN102432667A (en) | Nori protein polysaccharide product and counter-current pulse ultrasonic-assisted extraction method thereof | |
| CN102168111A (en) | Process method for producing alcohol by utilizing food residues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20121024 |