CN102432667A - Nori protein polysaccharide product and counter-current pulse ultrasonic-assisted extraction method thereof - Google Patents
Nori protein polysaccharide product and counter-current pulse ultrasonic-assisted extraction method thereof Download PDFInfo
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- CN102432667A CN102432667A CN2011103680408A CN201110368040A CN102432667A CN 102432667 A CN102432667 A CN 102432667A CN 2011103680408 A CN2011103680408 A CN 2011103680408A CN 201110368040 A CN201110368040 A CN 201110368040A CN 102432667 A CN102432667 A CN 102432667A
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Abstract
The invention provides a nori protein polysaccharide product and a counter-current pulse ultrasonic-assisted extraction method thereof, and relates to the field of the development and utilization of marine plant products. The product contains 5 to 16 percent of protein and 17 to 22 percent of polysaccharide. The method comprises the following steps of: crushing dry nori powder into particles of 40 meshes, adding tap water in an amount which is 40 to 67 times the weight of the nori powder into the nori powder and dissolving; adjusting the temperature of material liquid to 30 to 50 DEG C, and regulating the pH value to 9.0; performing pulsed counter-current ultrasonic-assisted water extraction according to the specific parameters that the ultrasonic pulse width is 1 to 5s, the pulse interval is 1 to 5s, the ultrasonic power provided for each gram of raw material is 19W and the ultrasonic frequency is 20kHz, and allowing the material liquid to pass through an ultrasonic probe in a counter-current circulation mode, wherein ultrasonic extraction time is 60 to 100min; and centrifuging to obtain supernatant, concentrating the supernatant, and spray-drying to obtain the product, wherein the air inlet temperature is 130 to 140 DEG C and the air outlet temperature is 90 to 95 DEG C.
Description
Technical field
The method of purple laver protein polysaccharide product and adverse current impulse ultrasound assisted extraction thereof relates to the utilization of marine plant Products Development, belongs to laver deep process technology field.
Technical background
China is the first in the world laver big producing country, and Jiangsu Province is China first laver big producing province.Contain rich in protein, carbohydrate, lipid acid, VITAMINs, inorganic elements etc. in the laver, nutritive value is abundant.Polysaccharide content is up to about 30% in the yezoensis laver, and protein content is up to more than 40%.Research about laver nutritive and active function both at home and abroad mainly concentrates on laver amylose.
Great deal of experimental shows, laver amylose has obvious effect at reducing blood-fat, anticoagulation and antithrombotic formation, immunomodulatory, mutation and aspect such as antitumor, anti-ageing, hypoglycemic.In addition, the phycoerythrin in the laver has been widely used in fields such as medicine, fluoroimmunoassay, foods and cosmetics, is a kind of important physical active substance.
Present most of laver is just directly edible behind the simple drying and moulding, seldom carries out further deep processing.Processing to laver at present also rests on the preliminary working stage; Scientific and technological content is low; The product profit rate is not high, and all is to be directed against single product about from laver, extracting albumen or STUDY ON POLYSACHAROSE at present, does not almost extract albumen and STUDY ON POLYSACHAROSE simultaneously; Just residue is abandoned it after extracting single product, caused serious wastage of material.
Laver albumen generally adopts potass extraction or enzymic hydrolysis extraction method, and laver amylose generally adopts hot water extraction or potass extraction method, but these two kinds the method extracting cycle is long, yield is low.
Adopt ultrasonic assisted extraction that extraction effect is improved greatly; And adopt general ultrasonic auxiliary extraction technology to have energy shortcoming pockety in raw material; The present invention adopts adverse current impulse ultrasound auxiliary extraction technology that polysaccharide in the yezoensis laver and albumen are extracted simultaneously, and energy is distributed in the raw material more uniformly.Thereby can improve energy utilization ratio, help save energy, raise the efficiency.
Summary of the invention
The present invention provides a kind of purple laver protein polysaccharide product and adverse current impulse ultrasound thereof auxiliary process for extracting simultaneously in order to overcome the deficiency of above-mentioned prior art.
A kind of purple laver protein polysaccharide product of the present invention, this product contains protein 5%～16%, polysaccharide 17%～22%.
The auxiliary process for extracting simultaneously of purple laver protein polysaccharide product adverse current impulse ultrasound of the present invention, carry out according to following step:
(1) dried laver powder is broken into 40 orders, in laver powder, adds 40 ~ 67 times of tap water dissolvings to laver powder weight; The flavouring liquid temperature is 30 ~ 50 ℃, transfers pH9.0;
(2) adopt adverse current impulse ultrasound assisted extraction; Concrete parameter is that pulses width 1 ~ 5s, recurrent interval 1 ~ 5s, every gram raw material provide ultrasonic power 19 W, ultrasonic frequency 20 kHz; Feed liquid is passed through ultrasound probe, supersound extraction times 60 ~ 100 min with the mode of circulated in countercurrent;
(3) after extraction finishes, the centrifuging and taking supernatant, with supernatant concentration, spraying drying obtains the purple laver protein polysaccharide product.
Advantage of the present invention:
The present invention extracts albumen and the polysaccharide in the laver simultaneously; With laver albumen and polysaccharide total yield is index; Optimize the extraction process condition, reach and shorten extracting cycle and the purpose that improves total extraction yield, for the suitability for industrialized production of high added value laver albumen and polysaccharide product provides a practical way.
The present invention adopts adverse current impulse ultrasound auxiliary extraction technology that polysaccharide in the yezoensis laver and albumen are extracted simultaneously, and more general ultrasonic assisted extraction method more evenly distributes energy effectively in extracting solution.
Description of drawings
Fig. 1 is an energy gathering type adverse current supersound extraction equipment structure chart, 1 ultrasound probe, and 2 contain liquid device, 3 opening for feeds, 4 ultrasonic controller, 5 recycle pumps, 6 discharge ports, 7 treatment solutions.
Embodiment
Weigh the reaction efficiency of reaction among the present invention with the total yield of albumen and polysaccharide, total yield is defined as the ratio that the ratio of pure protein quality and raw materials quality in the crude protein adds holosaccharide quality and raw materials quality in the Crude polysaccharides.The mensuration of protein content adopts forint phenol colourimetry in the crude protein; Phenol sulfuric acid colourimetry is adopted in measurement of the polysaccharide content in the Crude polysaccharides.
Fig. 1 is the energy gathering type adverse current supersound extraction equipment that the ultrasonic auxiliary water of adverse current of the present invention is proposed employing, and this equipment is furnished with ultrasonic wave controller 4, can send the UW that frequency is 20 kHz; Ultrasound probe 1 inserts the liquid of containing in the liquid device 2 wherein 7 that is processed and carries out supersound extraction, and ultransonic 5 pairs of the recycle pumps that start simultaneously are processed liquid and circulate, and are processed liquid from opening for feed 3 chargings, and from discharge port 6 dischargings, circulation gets into contains liquid device 2.Simultaneously can be through No. 4 control of ultrasonic controller ultrasound probe working current, PW, recurrent interval and supersound extraction total time.
Reference examples: the conventional extraction
Take by weighing the dried laver of 1000g, be crushed to 40 orders, get the 16g laver powder, add the tap water dissolving by solid-liquid ratio 1:50; Be heated to 40 ℃, regulate pH to 9.0, lixiviate 80min; The centrifugal 10min of 5000 rpm gets supernatant, concentrates; With its spraying drying, obtain purple laver protein polysaccharide powder, recording wherein, the total yield of laver pure protein and polysaccharide is 17.22%.
Embodiment 1:
Take by weighing the dried laver of 1000g, be crushed to 40 orders, get the 16g laver powder, add the tap water dissolving, be heated to 30 ℃, regulate pH to 9.0, carry out the ultrasonic assisted extraction of adverse current of laver albumen and polysaccharide by solid-liquid ratio 1:50.The ultrasonic assisted extraction of adverse current is taked intermittent type to apply hyperacoustic mode to carry out; The condition of extracting is: extraction time 100min, ultrasonic pulse fabric width 4s, recurrent interval 2s; Feed liquid is passed through ultrasound probe with the mode of circulated in countercurrent, and every gram raw material provides ultrasonic power 19W.After extracting end,, get supernatant, concentrate the back spraying drying, obtain purple laver protein polysaccharide powder the centrifugal 10min of extracting solution 5000 rpm.The total yield that records laver pure protein and polysaccharide is 30.24%, and wherein the yield of pure protein and holosaccharide is respectively 10.87% and 19.37%.
Embodiment 2:
The test treating processes is with embodiment 1, and its difference is that wherein solid-liquid ratio is 1:40, and temperature is 50 ℃, and extraction time is 80min, and the total yield of laver pure protein and polysaccharide is 25.83%, and wherein the yield of pure protein and holosaccharide is respectively 6.65% and 19.18%.
Embodiment 3:
The test treating processes is with embodiment 1, and its difference is that wherein solid-liquid ratio is 1:67, and temperature is 40 ℃, and extraction time is 60min, and the total yield of laver pure protein and polysaccharide is 29.95%, and wherein the yield of pure protein and holosaccharide is respectively 11.86% and 18.09%.
Embodiment 4:
The test treating processes is with embodiment 1, and its difference is that wherein temperature is 40 ℃, and extraction time is 80min, and the total yield of laver pure protein and polysaccharide is 36.70%, and wherein the yield of pure protein and holosaccharide is respectively 15.25% and 21.45%.
Embodiment 5:
The test treating processes is with embodiment 1, and its difference is that wherein solid-liquid ratio is 1:40, and temperature is 40 ℃, and extraction time is 60min, and the total yield of laver pure protein and polysaccharide is 25.50 %, and wherein the yield of pure protein and holosaccharide is respectively 7.47% and 18.03%.
Embodiment 6:
The test treating processes is with embodiment 1, and its difference is that wherein solid-liquid ratio is 1:67, and temperature is 50 ℃, and extraction time is 80min, and the total yield of laver pure protein and polysaccharide is 32.29%, and wherein the yield of pure protein and holosaccharide is respectively 12.97% and 19.32%.
Embodiment 7:
The test treating processes is with embodiment 1, and its difference is that wherein temperature is 40 ℃, and extraction time is 80min, ultrasonic pulse width 1s.The total yield of laver pure protein and polysaccharide is 31.79%, and wherein the yield of pure protein and holosaccharide is respectively 12.23% and 19.56%.
Embodiment 8:
The test treating processes is with embodiment 1, and its difference is that wherein temperature is 40 ℃, and extraction time is 80min, ultrasonic pulse width 5s.The total yield of laver pure protein and polysaccharide is 36.52%, and wherein the yield of pure protein and holosaccharide is respectively 14.42% and 22.10%.
Embodiment 9:
The test treating processes is with embodiment 1, and its difference is that wherein temperature is 40 ℃, and extraction time is 80min, recurrent interval 1s.The total yield of laver pure protein and polysaccharide is 36.88%, and wherein the yield of pure protein and holosaccharide is respectively 14.91% and 21.97%.
Embodiment 10:
The test treating processes is with embodiment 1, and its difference is that wherein temperature is 40 ℃, and extraction time is 80min, recurrent interval 5s.The total yield of laver pure protein and polysaccharide is 32.28%, and wherein the yield of pure protein and holosaccharide is respectively 12.99% and 19.29%.
Table 1 different embodiment laver albumen and polysaccharide total yield
| Processing mode | Total yield (%) |
| Comparative Examples | 17.22 |
| Embodiment 1 | 30.24 |
| Embodiment 2 | 25.83 |
| Embodiment 3 | 29.95 |
| Embodiment 4 | 36.70 |
| Embodiment 5 | 25.49 |
| Embodiment 6 | 32.29 |
| Embodiment 7 | 31.79 |
| Embodiment 8 | 36.52 |
| Embodiment 9 | 36.88 |
| Embodiment 10 | 32.28 |
Can find out the ultrasonic auxiliary extraction effect that can significantly improve laver albumen and polysaccharide than conventional method of extraction of adverse current by table 1.
Claims (2)
1. the purple laver protein polysaccharide product is characterized in that containing protein 5% ~ 16%, polysaccharide 17% ~ 22%.
2. purple laver protein polysaccharide product adverse current impulse ultrasound is assisted process for extracting simultaneously, it is characterized in that carrying out according to following step:
(1) dried laver powder is broken into 40 orders, in laver powder, adds 40 ~ 67 times of tap water dissolvings to laver powder weight; The flavouring liquid temperature is 30 ~ 50 ℃, transfers pH9.0;
(2) adopt adverse current impulse ultrasound assisted extraction; Concrete parameter is that pulses width 1 ~ 5s, recurrent interval 1 ~ 5s, every gram raw material provide ultrasonic power 19 W, ultrasonic frequency 20 kHz; Feed liquid is passed through ultrasound probe, supersound extraction times 60 ~ 100 min with the mode of circulated in countercurrent;
(3) after extraction finishes, the centrifuging and taking supernatant, with supernatant concentration, spraying drying obtains the purple laver protein polysaccharide product.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860538A (en) * | 2012-09-03 | 2013-01-09 | 江苏大学 | Alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method for seaweed protein and polysaccharide mixed product |
| CN103223261A (en) * | 2013-04-22 | 2013-07-31 | 华南理工大学 | Apparatus and method for pulsed electric field-assisted countercurrent extraction of grape skin residue polyphenol |
| CN103724398A (en) * | 2013-12-26 | 2014-04-16 | 江苏大学 | Method for extracting and purifying recombinant proteins |
| CN103907877A (en) * | 2014-04-01 | 2014-07-09 | 江南大学 | Method for preparing ginger extract with aid of efficient and circulatory pulsed ultrasound wave |
| CN114984613A (en) * | 2022-05-30 | 2022-09-02 | 青岛安美瑞生物科技产业园股份有限公司 | Plant polysaccharide extraction and drying process and device for implementing process |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102860538A (en) * | 2012-09-03 | 2013-01-09 | 江苏大学 | Alternate double-frequency countercurrent ultrasonic-assisted simultaneous extraction method for seaweed protein and polysaccharide mixed product |
| CN103223261A (en) * | 2013-04-22 | 2013-07-31 | 华南理工大学 | Apparatus and method for pulsed electric field-assisted countercurrent extraction of grape skin residue polyphenol |
| CN103223261B (en) * | 2013-04-22 | 2014-12-31 | 华南理工大学 | Apparatus and method for pulsed electric field-assisted countercurrent extraction of grape skin residue polyphenol |
| CN103724398A (en) * | 2013-12-26 | 2014-04-16 | 江苏大学 | Method for extracting and purifying recombinant proteins |
| CN103724398B (en) * | 2013-12-26 | 2016-06-15 | 江苏大学 | A kind of method for extraction and purification of recombiant protein |
| CN103907877A (en) * | 2014-04-01 | 2014-07-09 | 江南大学 | Method for preparing ginger extract with aid of efficient and circulatory pulsed ultrasound wave |
| CN103907877B (en) * | 2014-04-01 | 2016-01-20 | 江南大学 | A kind of efficient circulation pulse ultrasonic wave assists the method preparing Ginger P.E |
| CN114984613A (en) * | 2022-05-30 | 2022-09-02 | 青岛安美瑞生物科技产业园股份有限公司 | Plant polysaccharide extraction and drying process and device for implementing process |
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Application publication date: 20120502 |