CN105325801A - Preparation method of sea cucumber intestinal peptide - Google Patents
Preparation method of sea cucumber intestinal peptide Download PDFInfo
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- CN105325801A CN105325801A CN201410400885.4A CN201410400885A CN105325801A CN 105325801 A CN105325801 A CN 105325801A CN 201410400885 A CN201410400885 A CN 201410400885A CN 105325801 A CN105325801 A CN 105325801A
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Abstract
The invention relates to a preparation method of sea cucumber intestinal peptide. The method comprises the following steps: taking waste sea cucumber intestine as a raw material which is not processed with sands removal, continuously cleaning the raw material, taking the cleaned sea cucumber intestine out, separating the cleaned water body to respectively obtain a clear supernatant and sedimentary sludge, heating the sedimentary sludge and performing an protease enzymatic hydrolysis technology on the sedimentary sludge for extracting the sea cucumber intestinal peptide, performing protease enzymatic hydrolysis on the cleaned sea cucumber intestine for conversing protein to the low molecular weight sea cucumber intestinal peptide, adding yeast powder in a sea cucumber intestine enzymatic hydrolysate for fermentation, and finally concentrating the product and drying the product to prepare the sea cucumber intestinal peptide powder. The product has smell and nutrition of the sea cucumber intestine, and can be used for preparing various functional foods. The preparation method of sea cucumber intestinal peptide is characterized in that waste sea cucumber intestine is fully used, so that sea cucumber intestine waste can be effectively used, environment pollution problem due to waste is reduced, and nutrition component of the sea cucumber can be kept at maximum degree. The steps during the processing engineering have the advantages of reasonable and simple arrangement, and low cost; and the product is the green non-pollution product.
Description
Technical field
The invention belongs to marine byproduct manufacture field, relate to sea cucumber intestine and go the technical fields such as sand, enzymolysis, fermentation and drying, the husky sea cucumber intestine of band is utilized, saves production cost.
Background technology
Sea cucumber is a kind ofly of value to healthy food, containing multiple biological regulation material, as Multiple components such as antitumorigenic substance, anti arteriosclerosis, anti-inflammatory, raising immunity.In recent years, along with Chinese sea cucumber industry develops rapidly, market and cultivation scale constantly expand, corresponding problem is, sea cucumber comprehensive utilization degree is on the low side, and Holothurian machining is only only limitted to wall of sea cucumber Stichopus japonicus, leftover bits and pieces sea cucumber intestine in process in process because it carries a large amount of silt, often be taken as Fei Qi Wu Lost to abandon, developed fully, cause the wasting of resources and environmental pollution.The annual national output of sea cucumber is 14-15 ten thousand tons, and sea cucumber alive per ton about can process clean sea cucumber intestine 16 kilograms, and namely sea cucumber intestine can reach 2300 tons.Therefore in order to open up sea cucumber comprehensive processing and utilization, meeting market demand, increasing economic well-being of workers and staff, needing the method processing and utilization sea cucumber intestine finding a kind of high efficiency low cost badly.
Modern nutriology analytical proof, sea cucumber intestine not only contains same abundant active material with wall of sea cucumber Stichopus japonicus, and many precious nutrient contents are higher, as polysaccharide, vanadium, nucleic acid, arginine etc.; Sea cucumber intestine is also containing the very abundant composition such as pigment (bata-carotene, echinenone, astaxanthin, zeaxanthin, canthaxanthin and some unknown pigments), stichopus japonicus lipid, stichopus japonicus saponin, selenium, zinc, iron.Pigment in sea cucumber intestine, self can not synthesize in animal body, has non-oxidizability, and the free radical in energy purged body, delays senility.In sea cucumber intestine, the content of vanadium is 3-4 times of wall of sea cucumber Stichopus japonicus, and vanadium can maintain the normal carbohydrate metabolism of body, effectively prevents and treats diabetes, stimulates the hematopoiesis function of body, promotes the hematopoietic potential of bone.The nutritional labeling of sea cucumber intestine is very abundant, especially Sea Cucumber Glycosides, also be called holothurin by some, it can antitumor, radioresistance, antimycotic, anti-cell toxicity, cholinolytic effect, can inhibition cancer cell growth and transfer, the transmission that affects the nerves, all have good effect to alpastic anemia, diabetes, gastric ulcer etc.Sea cucumber polysaccharide also has several functions activity, plays an important role in the vital movement of body.As can be seen here, sea cucumber intestine has very high using value.
Summary of the invention
For the problems referred to above, the object of this invention is to provide a kind of simple, satisfactory for result, environmental protection, make full use of the husky sea cucumber intestine of band and prepare sea cucumber intestine peptide.
The present invention is directed to the husky sea cucumber intestine discarded object of band, provide a kind of preparation method of sea cucumber intestine peptide, its object is to the utilization rate improving sea cucumber intestine, concrete grammar is as follows:
(1) take the husky sea cucumber intestine of a certain amount of band, add a certain amount of water wherein, pull sea cucumber intestine out after slight rinsing 5-10min, cleaning fluid is abandoned;
(2) sea cucumber intestine pulled out is placed in 5-30 times of clear water stirring and washing or air bubble washer 10-30min continuously, pulls sea cucumber intestine out, cleaning fluid, in the centrifugal 5-10min of 500-2000r/min, collects cleaning supernatant, and precipitation mud is continued to employ;
(3) in the sea cucumber intestine of step (2), above-mentioned cleaning supernatant is added, continuous stirring and washing or air bubble washer 10-30min again, pull sea cucumber intestine out, cleaning fluid is in the centrifugal 5-10min of 500-2000r/min, gained cleaning supernatant is continued to employ, and precipitation mud continues following operation;
(4) the precipitation mud of step (2) and (3) is merged, add the clear water of 2-10 times of volume, fully under the condition of 80 DEG C-100 DEG C, heat 5-20min after mixing, be cooled to room temperature;
(5) in the treatment fluid of step (4), add protease by the dosage of every gram of sea cucumber intestine raw material 10-100U, the centrifugal 5-10min of enzymolysis processing 20min-5h, 500-2000r/min under 40-60 DEG C of shaker water bath condition, obtain enzymolysis supernatant and precipitation mud; Enzymolysis supernatant is continued to employ, and precipitation mud adds a small amount of clear water again and carries out centrifuge washing, after repeatedly processing 2-5 time, collects the supernatant after whole centrifuge washing;
(6) the enzymolysis supernatant adding step (5) after being ground by the sea cucumber intestine cleaned up carries out enzymolysis, and in 40-60 DEG C of shaker water bath, the centrifugal 5-10min of enzymolysis processing 20min-5h, 500-2000r/min goes precipitation;
(7) by step (3), the mixing of (5) and (6) gained supernatant, in 40-60 DEG C of shaker water bath, continue enzymolysis processing 20min-5h, 2000-5000r/min high speed centrifugation 10-20min, discards precipitation, obtains sea cucumber intestine enzymolysis liquid;
(8) in sea cucumber intestine enzymolysis liquid, the dusty yeast that percentage by weight is 0.1%-1% is added, in 28-32 DEG C of condition bottom fermentation 0.5-5h; Rotary evaporation carries out spraying dry and obtains sea cucumber intestine peptide after being concentrated into the 20-50% of original volume.
Advantage of the present invention: 1) utilize this method to prepare sea cucumber intestine peptide, through sedimentation repeatedly and centrifugal, can remove fine sand completely.2) in preparation process, only use water and protease, safety non-pollution, the destruction of ecological environment can not be caused, 3) reduce the consumption of water and protease in preparation process as much as possible, reduce costs as much as possible.4) processing technology is extracted and enzymolysis processing by repeatedly cleaning, heat, farthest extracts sea cucumber and can utilize composition, ensure resource utilization.5) improve the utilization rate of Holothurian machining byproduct in process thing, nutritional labeling contained by it and function factor are fully developed.6) with the addition of dusty yeast in the enzymolysis liquid that the present invention obtains and carry out fermentation except smelling removal, make it keep good local flavor.
The present invention is in whipping process, and stir speed (S.S.) can suitably adjust, and what period can be suitable takes measures sea cucumber intestine is disperseed, and makes silt as far as possible out.
Silt collected by the present invention, needs repeatedly to add water treatment, makes the sea cucumber nutrient material stripping as far as possible in silt, utilizes sea cucumber intestine to greatest extent.
Method provided by the present invention all can be removed silt, guarantees that sea cucumber intestine enzymolysis liquid is without sand.
Protease involved in the present invention comprises neutral proteinase, the multiple protein enzymes such as pancreatin, alkali protease, flavor protease.
Accompanying drawing explanation
Fig. 1: flow chart prepared by sea cucumber intestine peptide.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described, but the present invention is not limited to following examples.
Embodiment 1:
(1) take 10.5g and be with husky sea cucumber intestine, add the water of 10mL wherein, pull sea cucumber intestine out after slight rinsing 5min, cleaning fluid is abandoned;
(2) sea cucumber intestine pulled out is placed in 20ml clear water stirring and washing 20min continuously, pulls sea cucumber intestine out, cleaning fluid, in the centrifugal 5min of 1000r/min, collects cleaning supernatant, and precipitation mud is continued to employ;
(3) in the sea cucumber intestine of step (2), add above-mentioned cleaning supernatant, continuous stirring and washing 20min, pulls sea cucumber intestine out again, and cleaning fluid is in the centrifugal 5min of 1000r/min, and gained cleaning supernatant is continued to employ, and precipitation mud continues following operation;
(4) the precipitation mud of step (2) and (3) is merged, add the clear water of 15mL, fully under the condition of 90 DEG C, heat 10min after mixing, be cooled to room temperature;
(5) in the treatment fluid of step (4), add neutral proteinase by the dosage of every gram of sea cucumber intestine raw material 40U, the centrifugal 5min of enzymolysis processing 2h, 1000r/min under 50 DEG C of shaker water bath conditions, obtain enzymolysis supernatant and precipitation mud; Enzymolysis supernatant is continued to employ, and precipitation mud adds 10mL clear water again and carries out centrifuge washing, after repeatedly processing 3 times, collects the supernatant after whole centrifuge washing;
(6) the enzymolysis supernatant adding step (5) after being ground by the sea cucumber intestine cleaned up carries out enzymolysis, and in 50 DEG C of shaker water bath, the centrifugal 5min of enzymolysis processing 2h, 1000r/min goes precipitation;
(7) by step (3), the mixing of (5) and (6) gained supernatant, in 50 DEG C of shaker water bath, continue enzymolysis processing 2h, 3000r/min high speed centrifugation 10min, discards precipitation, obtains sea cucumber intestine enzymolysis liquid;
(8) in sea cucumber intestine enzymolysis liquid, the dusty yeast that percentage by weight is 0.2% is added, in 32 DEG C of condition bottom fermentation 1h; Rotary evaporation carries out spraying dry and obtains sea cucumber intestine peptide after being concentrated into 50% of original volume;
Sea cucumber intestine enzymolysis liquid protein recovery obtained according to the method described above can reach 82.7%.
Embodiment 2:
(1) take 20g and be with husky sea cucumber intestine, add the water of 20mL wherein, pull sea cucumber intestine out after slight rinsing 10min, cleaning fluid is abandoned;
(2) sea cucumber intestine pulled out is placed in 30mL clear water stirring and washing 30min continuously, pulls sea cucumber intestine out, cleaning fluid, in the centrifugal 5-10min of 500r/min, collects cleaning supernatant, and precipitation mud is continued to employ;
(3) in the sea cucumber intestine of step (2), add above-mentioned cleaning supernatant, continuous stirring and washing or air bubble washer 15min, pull sea cucumber intestine out again, and cleaning fluid is in the centrifugal 5min of 1500r/min, and gained cleaning supernatant is continued to employ, and precipitation mud continues following operation;
(4) the precipitation mud of step (2) and (3) is merged, add the clear water of 20mL, fully under the condition of 100 DEG C, heat 15min after mixing, be cooled to room temperature;
(5) in the treatment fluid of step (4), add animal proteolytic enzyme by the dosage of every gram of sea cucumber intestine raw material 60U, the centrifugal 10min of enzymolysis processing 3h, 1000r/min under 50 DEG C of shaker water bath conditions, obtain enzymolysis supernatant and precipitation mud; Enzymolysis supernatant is continued to employ, and precipitation mud adds 15mL clear water again and carries out centrifuge washing, after repeatedly processing 4 times, collects the supernatant after whole centrifuge washing;
(6) the enzymolysis supernatant adding step (5) after being ground by the sea cucumber intestine cleaned up carries out enzymolysis, and in 50 DEG C of shaker water bath, the centrifugal 5min of enzymolysis processing 3h, 1500r/min goes precipitation;
(7) by step (3), the mixing of (5) and (6) gained supernatant, in 50 DEG C of shaker water bath, continue enzymolysis processing 1h, 4000r/min high speed centrifugation 15min, discards precipitation, obtains sea cucumber intestine enzymolysis liquid;
(8) in sea cucumber intestine enzymolysis liquid, the dusty yeast that percentage by weight is 0.25% is added, in 30 DEG C of condition bottom fermentation 2h; Rotary evaporation carries out spraying dry and obtains sea cucumber intestine peptide after being concentrated into 30% of original volume;
Sea cucumber intestine enzymolysis liquid protein recovery obtained according to the method described above can reach 83.6%.
Carry out amino acid composition analysis by collecting the sea cucumber intestine peptide obtained, amino acid composition analysis result is as follows:
Note: * is the necessary amino acid of human body.
Claims (5)
1. the preparation method of a sea cucumber intestine peptide, with without going the discarded sea cucumber intestine of husky process for raw material, raw material is after continuous wash, pull the sea cucumber intestine cleaned up out, water body after cleaning obtains cleaning supernatant and precipitation mud respectively after being separated, precipitation mud extracts sea cucumber intestine peptide wherein further by heating and protease hydrolyzed technique, protein transduction is turned to low molecular weight sea cucumber intestines peptide by protease hydrolyzed by the sea cucumber intestine cleaned up, sea cucumber intestine enzymolysis liquid adds dusty yeast again and ferments, and last concentrate drying makes sea cucumber intestine Gly-His-Lys.
2. according to the preparation method of a kind of sea cucumber intestine peptide according to claim 1, it is characterized in that described continuous wash is: take the husky sea cucumber intestine of a certain amount of band, add a small amount of clear water wherein, sea cucumber intestine is pulled out after slight rinsing 5-10min, be placed in 5-30 times of clear water stirring and washing or air bubble washer 10-30min continuously again, pull sea cucumber intestine out.
3. according to the preparation method of a kind of sea cucumber intestine peptide according to claim 1, it is characterized in that described heating is: the clear water adding 2-10 times of volume in the precipitation mud of cleaning sea cucumber, fully under the condition of 80 DEG C-100 DEG C, heat 5-20min after mixing.
4. according to the preparation method of a kind of sea cucumber intestine peptide according to claim 1, it is characterized in that described protease hydrolyzed is: in the precipitation mud of cleaning sea cucumber, protease is added by the dosage of every gram of sea cucumber intestine raw material 10-100U, enzymolysis processing 20min-5h under 40-60 DEG C of shaker water bath condition, the centrifugal 5-10min of 500-2000r/min, obtain enzymolysis supernatant and precipitation mud, add above-mentioned enzymolysis supernatant after being ground by the sea cucumber intestine cleaned up and carry out enzymolysis, complete after enzymolysis processing 20min-5h in 40-60 DEG C of shaker water bath.
5. according to the preparation method of a kind of sea cucumber intestine peptide according to claim 1, it is characterized in that described fermentation is: in sea cucumber intestine enzymolysis liquid, add the dusty yeast that percentage by weight is 0.1%-1%, in 28-32 DEG C of condition bottom fermentation 0.5-5h.
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Cited By (10)
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| CN106539075A (en) * | 2016-07-18 | 2017-03-29 | 陈伟宁 | A kind of preparation method of efficient absorption sea cucumber nutrient buccal tablet |
| CN107549682A (en) * | 2017-09-28 | 2018-01-09 | 句容市武杭传动机械制造有限公司 | The processing method of a fermentation cucumber product |
| CN109259215A (en) * | 2018-10-08 | 2019-01-25 | 北京国康本草物种生物科学技术研究院有限公司 | A kind of active material of sea cucumber and its separating and extracting process |
| CN110269250A (en) * | 2019-07-19 | 2019-09-24 | 蓬莱深奥生物科技研究所 | A kind of wall of sea cucumber Stichopus japonicus ferment and preparation method thereof |
| CN110973342A (en) * | 2020-03-05 | 2020-04-10 | 中国科学院烟台海岸带研究所 | Apostichopus japonicus oligopeptide and preparation method and application thereof |
| CN111592582A (en) * | 2020-06-29 | 2020-08-28 | 中食都庆（山东）生物技术有限公司 | Sea cucumber intestinal peptide and preparation method and application thereof |
| CN112690387A (en) * | 2020-12-24 | 2021-04-23 | 大连鑫澜生物技术有限公司 | Sea cucumber intestine extract probiotic drink capable of strengthening kidney and strengthening body |
| CN112826093A (en) * | 2021-02-02 | 2021-05-25 | 集美大学 | Method for removing fishy smell of sea cucumber intestinal peptide powder |
| CN113004384A (en) * | 2021-02-20 | 2021-06-22 | 中国海洋大学 | Preparation method and application of sea cucumber intestine bone-promoting peptide |
| CN114504083A (en) * | 2020-11-16 | 2022-05-17 | 烟台东宇海珍品有限公司 | Processing method of sea cucumber sausages |
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| CN112690387A (en) * | 2020-12-24 | 2021-04-23 | 大连鑫澜生物技术有限公司 | Sea cucumber intestine extract probiotic drink capable of strengthening kidney and strengthening body |
| CN112826093A (en) * | 2021-02-02 | 2021-05-25 | 集美大学 | Method for removing fishy smell of sea cucumber intestinal peptide powder |
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| CN113004384A (en) * | 2021-02-20 | 2021-06-22 | 中国海洋大学 | Preparation method and application of sea cucumber intestine bone-promoting peptide |
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