CN101361578A - Beche-de-mer ovum nurture and preparation method thereof - Google Patents
Beche-de-mer ovum nurture and preparation method thereof Download PDFInfo
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- CN101361578A CN101361578A CNA2008100134265A CN200810013426A CN101361578A CN 101361578 A CN101361578 A CN 101361578A CN A2008100134265 A CNA2008100134265 A CN A2008100134265A CN 200810013426 A CN200810013426 A CN 200810013426A CN 101361578 A CN101361578 A CN 101361578A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 102000002322 Egg Proteins Human genes 0.000 title claims description 59
- 108010000912 Egg Proteins Proteins 0.000 title claims description 59
- 210000004681 ovum Anatomy 0.000 title claims description 59
- 241000251511 Holothuroidea Species 0.000 claims abstract description 59
- 102000004190 Enzymes Human genes 0.000 claims abstract description 44
- 108090000790 Enzymes Proteins 0.000 claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 210000000936 intestine Anatomy 0.000 claims abstract description 31
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000004108 freeze drying Methods 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 11
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 238000005342 ion exchange Methods 0.000 claims abstract description 3
- 229940088598 enzyme Drugs 0.000 claims description 39
- 239000006228 supernatant Substances 0.000 claims description 33
- 230000007062 hydrolysis Effects 0.000 claims description 20
- 238000006460 hydrolysis reaction Methods 0.000 claims description 20
- 239000012141 concentrate Substances 0.000 claims description 19
- 102000057297 Pepsin A Human genes 0.000 claims description 18
- 108090000284 Pepsin A Proteins 0.000 claims description 18
- 229940111202 pepsin Drugs 0.000 claims description 18
- 238000003756 stirring Methods 0.000 claims description 12
- 238000005341 cation exchange Methods 0.000 claims description 11
- 238000005119 centrifugation Methods 0.000 claims description 11
- 238000001556 precipitation Methods 0.000 claims description 11
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 11
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 10
- 229910052785 arsenic Inorganic materials 0.000 claims description 10
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 claims description 10
- 244000052616 bacterial pathogen Species 0.000 claims description 10
- 229910052793 cadmium Inorganic materials 0.000 claims description 10
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 10
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 10
- 229910052753 mercury Inorganic materials 0.000 claims description 10
- 230000009965 odorless effect Effects 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 230000009967 tasteless effect Effects 0.000 claims description 10
- 230000001105 regulatory effect Effects 0.000 claims description 8
- 238000007711 solidification Methods 0.000 claims description 8
- 230000008023 solidification Effects 0.000 claims description 8
- 230000007935 neutral effect Effects 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 5
- 239000003513 alkali Substances 0.000 claims description 5
- 235000019419 proteases Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 238000001694 spray drying Methods 0.000 claims description 4
- 238000013467 fragmentation Methods 0.000 claims description 3
- 238000006062 fragmentation reaction Methods 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 2
- 238000000034 method Methods 0.000 abstract description 8
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 2
- 108010051873 alkaline protease Proteins 0.000 abstract 1
- 108010007093 dispase Proteins 0.000 abstract 1
- 238000003672 processing method Methods 0.000 abstract 1
- 235000008504 concentrate Nutrition 0.000 description 16
- 239000000047 product Substances 0.000 description 15
- 230000002358 autolytic effect Effects 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a sea cucumber egg nutriment and a preparation method thereof. The sea cucumber egg nutriment is white or light yellow hydroscopic powder free of odor and taste and highly soluble to water and ethanol. The preparation method comprises sea cucumber egg and intestine breaking and enzymolysis, metal ion removal through ion exchange, and freeze drying, wherein the enzymolysis employs conjugated enzymes of exogenous enzymes and endogenous enzymes or single exogenous enzymes; single exogenous enzyme enzymolysis refers to the enzymolysis using single pepsinase or using the mixture of trypsinase, dispase and alkaline proteinase. The invention not only makes wastes in sea cucumber processing with traditional methods into an invigorant rich in nutrition but also reduces pollution of the wastes to the environment. The processing method is simple and reasonable and can be industrialized easily.
Description
Technical field
The invention belongs to from marine animal and extract the nutraceutical field, relate to simultaneously and adopt enzyme hydrolysis, chemicals ion-exchange, cryodesiccated technical field.
Background technology
The sea cucumber ovum is named sea cucumber again, and nutritive value is high, and the sea cucumber ovum is generally taken from the sea cucumber in every year 5, June, just because of acquisition time is of short duration, output is very low, so the sea cucumber ovum is very valuable, rare nourishing treasure.In aquatic products processing enterprise, sea cucumber is all when disposing as dead meal in the sea cucumber process so far, and a large amount of nutritional labelings and function factor are not fully utilized in the sea cucumber ovum, cause this rare resources to be wasted fully.
At present, there is not the Related product of sea cucumber ovum to come out as yet, do not see relevant document yet and close information dissemination.
Summary of the invention
At the situation that the sea cucumber ovum of the preciousness in the sea cucumber processing is underutilized, the present invention aims to provide a kind of sea cucumber ovum nutrition product and preparation method thereof.
Technical scheme of the present invention is: the method that adopts exogenous enzymes and endogenous autolytic enzyme to combine is handled the sea cucumber ovum, removes heavy metal ion with the cation exchange columns in series, is made into functional product by freezing or spray drying technology.Its concrete processing step is:
1. the processing of sea cucumber
The sea cucumber ovum is separated, cleans with the sea cucumber body wall with intestines, remove silt and impurity; Its fragmentation is standby;
2. enzymolysis processing
Can adopt endogenous autolytic enzyme and exogenous enzymes in conjunction with handling sea cucumber ovum and intestines, perhaps single with exogenous enzymes processing sea cucumber ovum and intestines; List also can or be used mixed enzyme with single pepsin with exogenous enzymes:
(1) adopt endogenous autolytic enzyme and exogenous enzymes in conjunction with processing: the water that the sea cucumber ovum after the break process and intestines is added 10～100 times of its quality mixes, and transferring pH is 3～8, places 30～50 ℃ of water-baths through endogenous enzymes processing reaction time 2～8h then.Sea cucumber ovum and intestines after endogenous autolytic enzyme is handled, the pepsin of adding solution quality 0.1～2.0% adopts continous way to stir enzymolysis 2～5h down at 30～70 ℃, keeps above-mentioned pH value during hydrolysis; Enzymolysis finishes and transfers pH to arrive neutrality again, through high temperature go out enzyme, being chilled to room temperature, 3000～8000r/min, centrifugal 10～30min gets supernatant, precipitation adds 10～50 times in water again and repeats pepsin step 1 time, the supernatant of centrifugation merges stand-by.
(2) adopt single exogenous enzymes to handle sea cucumber ovum and intestines: the water that sea cucumber ovum after break process and intestines add 10～100 times of its quality mixes, transferring pH is 3～8, the pepsin that adds solution quality 0.5～3.0%, adopt continous way to stir enzymolysis 3～8h down at 30～70 ℃, keep above-mentioned pH value during hydrolysis; Hydrolysis finishes and transfers pH to neutral, through high temperature go out enzyme, be chilled to room temperature, the centrifugal 10～30min of 3000～8000r/min, supernatant, precipitation adds water again and repeats above-mentioned steps 1～2 time for 10～50 times, the supernatant of centrifugation merges stand-by.
(3) enzymolysis can also carry out enzymolysis with trypsase, neutral proteinase and alkali protease mixed enzyme, and the ratio of three kinds of enzyme addings is 1:1～3:1～3.Enzymolysis process is the same.
More than the method for single external source enzyme resolving and mixed enzyme enzymolysis be applied to independent sea cucumber intestine or independent sea cucumber ovum processing more suitable.
3. enzymolysis liquid concentrates
With the centrifuged supernatant of above-mentioned gained, 20～60 ℃ are evaporated to 1/2～1/10 of original volume.
4. remove heavy metal ion
Remove the metal ion in the concentrate with faintly acid, alkalescent cation exchange columns in series, the control flow velocity is 100～1000ml/min.
5. dry
The concentrate of collecting is carried out freeze drying, and regulating solidification point is 20～40 ℃, and condenser temperature is-20～-40 ℃, and vacuum is 5～30Pa, be 1～6wt% to moisture till, promptly obtain beche-de-mer ovum nurture after the freeze-drying; Or to adopt spray-dired method, outlet temperature be 100～250 ℃, and air mass flow is 50～300m
3/ h.
Beche-de-mer ovum nurture of the present invention is white or faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
The advantage that the present invention gives prominence to is:
1. unemployed sea cucumber ovum processing in the conventional method processing is handled, produced the invigorant that is rich in nutritive value, also reduce the pollution of discarded object simultaneously environment.Beche-de-mer ovum nurture after processing is handled is rich in nutrients such as polypeptide, polysaccharide, is a kind of product of great exploitation potential for its.
2. adopt sea cucumber endogenous enzymes and exogenous enzymes in conjunction with handling the sea cucumber ovum, kept the active component of sea cucumber to greatest extent, obtain the more product of high-quality;
3. adopt exchanger resin that harmful metal ion is removed first, solved the problem of the easy enriching heavy metal of marine product, improve the safety and the quality of product.
The specific embodiment
Embodiment 1
1kg sea cucumber ovum is separated, shreds, cleans with body wall with intestines, mix shredding the water that sea cucumber ovum after the processing and intestines add its quality 50kg, transferring pH is 5, places 50 ℃ of water-bath reaction time 6h then.Sea cucumber ovum and intestines after endogenous enzymes is handled, the pepsin of adding 1kg adopts continous way to stir enzymolysis 2h down at 30 ℃, keeps above-mentioned pH value during hydrolysis; Transfer pH to arrive neutrality again, the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 30min of 3500r/min, supernatant, the water that the precipitation of 0.5kg adds 5kg again repeats the pepsin step, the supernatant of centrifugation merges stand-by; With the 50L centrifuged supernatant of above-mentioned gained, 35 ℃ are evaporated to 10L; Above-mentioned concentrate is handled pillar volume 1000ml, flow velocity 100ml/min with Subacidity cation exchange columns in series.The concentrate of collecting is carried out freeze drying, and regulating solidification point is 20 ℃, and condenser temperature is-20 ℃, and vacuum is 20Pa, to moisture be till 6%, promptly obtain beche-de-mer ovum nurture after the freeze-drying.It is the white solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 2
10kg sea cucumber ovum is separated, shreds, cleans with body wall with intestines, mix shredding the water that sea cucumber ovum after the processing and intestines add 100kg, transferring pH is 7, places 30 ℃ of water-bath reaction time 2h then.Sea cucumber ovum and intestines after endogenous enzymes is handled, the pepsin of adding 2kg adopts continous way to stir enzymolysis 5h down at 50 ℃, keeps above-mentioned pH value during hydrolysis; Transfer pH to arrive neutrality again, the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 10min of 4000r/min, supernatant, the water that the precipitation of 8kg adds 80kg again repeats the pepsin step, the supernatant of centrifugation merges stand-by; With the 150L centrifuged supernatant of above-mentioned gained, 40 ℃ are evaporated to 50L; Above-mentioned concentrate is handled pillar volume 5000ml, flow velocity 1000ml/min with Subacidity cation exchange columns in series.The concentrate of collecting is carried out freeze drying, and regulating solidification point is 30 ℃, and condenser temperature is-25 ℃, and vacuum is 25Pa, to moisture be till 5%, promptly obtain beche-de-mer ovum nurture after the freeze-drying.Product is the faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 3
100g sea cucumber ovum is separated, shreds, cleans with body wall with intestines, mix shredding the water that sea cucumber ovum after the processing and intestines add 8000g, transferring pH is 3, places 45 ℃ of water-bath reaction time 3h then.Sea cucumber ovum and intestines after endogenous enzymes is handled, the pepsin of adding 120g adopts continous way to stir enzymolysis 3h down at 50 ℃, keeps above-mentioned pH value during hydrolysis; Transfer pH to arrive neutrality again, the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 15min of 5000r/min, supernatant, the precipitation of 70g adds 140g water again and repeats the pepsin step, the supernatant of centrifugation merges stand-by; With the 7800ml centrifuged supernatant of above-mentioned gained, 60 ℃ are evaporated to 800ml; Above-mentioned concentrate is handled pillar volume 8000ml, flow velocity 1000ml/min with alkalescent cation exchange columns in series.The concentrate of collecting is carried out the concentrate of collecting is carried out spray-drying, and outlet temperature is 200 ℃, and air mass flow is 300m
3/ h promptly obtains beche-de-mer ovum nurture after the drying.Product is the faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 4 separates 500g sea cucumber ovum, shred, clean with body wall with intestines, the water that sea cucumber ovum after treatment adds 45000g mixes, and transferring pH is 4, adds the pepsin of 1350g, adopt continous way to stir enzymolysis 3h down at 55 ℃, keep above-mentioned pH value during hydrolysis; Transfer pH to arrive neutrality again, the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 25min of 6000r/min, supernatant, the 300g precipitation adds 6000g water again and repeats the pepsin step, the supernatant of centrifugation merges stand-by; With the 50000ml centrifuged supernatant of above-mentioned gained, 55 ℃ are evaporated to 5000ml; Above-mentioned concentrate is handled pillar volume 8000ml, flow velocity 500ml/min with alkalescent cation exchange columns in series.The concentrate of collecting is carried out freeze drying, and regulating solidification point is 35 ℃, and condenser temperature is-40 ℃, and vacuum is 20Pa, to moisture be till 3%, promptly obtain beche-de-mer ovum nurture after the freeze-drying.Product is the faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 5
100g sea cucumber ovum is separated, shreds, cleans with body wall with intestines, and the water that sea cucumber ovum after treatment and intestines add 6000g mixes, and transferring pH is 6, adds the pepsin of 90g, adopts continous way to stir enzymolysis 8h down at 70 ℃, keeps above-mentioned pH value during hydrolysis; Hydrolysis finishes and transfers pH to neutral, and the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 10min of 8000r/min, supernatant, the 50g precipitation adds water 900g again and repeats the pepsin step, the supernatant of centrifugation merges stand-by.With the 6000ml centrifuged supernatant of above-mentioned gained, 55 ℃ are evaporated to 1000ml; Above-mentioned concentrate is handled pillar volume 7000ml, flow velocity 500ml/min with alkalescent cation exchange columns in series.The concentrate of collecting is carried out freeze drying, and regulating solidification point is 40 ℃, and condenser temperature is-30 ℃, and vacuum is 30Pa, to moisture be till 5%, promptly obtain beche-de-mer ovum nurture after the freeze-drying.Product is the faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 6
100g sea cucumber ovum is separated, shreds, cleans with body wall with intestines, the water that sea cucumber ovum after treatment and intestines add 5000g mixes, transferring pH is 8, the mixed enzyme that adds 120g, wherein trypsase 20g, neutral proteinase 40g, alkali protease 60g, adopt continous way to stir enzymolysis 3h down at 60 ℃, keep above-mentioned pH value during hydrolysis; Hydrolysis finishes and transfers pH to neutral, and the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 15min of 7000r/min, supernatant 4500ml.With the centrifuged supernatant of above-mentioned gained, 55 ℃ are evaporated to 450ml; Handle pillar volume 10000ml, flow velocity 500ml/min with Subacidity cation exchange columns in series then.The concentrate of collecting is carried out freeze drying, and regulating solidification point is 35 ℃, and condenser temperature is-40 ℃, and vacuum is 25Pa, to moisture be till 1%, promptly obtain beche-de-mer ovum nurture after the freeze-drying.Product is the white solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 7
The 1kg sea cucumber intestine is separated, shreds, cleans with body wall, and the water that sea cucumber intestine after treatment adds 50kg mixes, and transferring pH is 8, adds the pepsin of 1.5kg, adopts continous way to stir enzymolysis 3h down at 60 ℃, keeps above-mentioned pH value during hydrolysis; Hydrolysis finishes and transfers pH to neutral, and the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 15min of 7000r/min, supernatant, the precipitation of 600g adds 3000g water again and repeats above complex enzyme treatment step, the supernatant of centrifugation merges stand-by.With the centrifuged supernatant of the 48L of above-mentioned gained, 55 ℃ are evaporated to 5L; Handle pillar volume 9000ml, flow velocity 800ml/min with Subacidity cation exchange columns in series then.The concentrate of collecting is carried out freeze drying, and regulating solidification point is 40 ℃, and condenser temperature is-40 ℃, and vacuum is 30Pa, to moisture be till 3%, promptly obtain the sea cucumber intestine nutriment after the freeze-drying.Product is the faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Embodiment 8
5kg sea cucumber ovum is separated, shreds, cleans with body wall, the water that sea cucumber ovum after treatment adds 250kg mixes, transferring pH is 8, the mixed enzyme that adds 5kg, wherein trypsase 1kg, neutral proteinase 2kg, alkali protease 2kg, adopt continous way to stir enzymolysis 4h down at 60 ℃, keep above-mentioned pH value during hydrolysis; Hydrolysis finishes and transfers pH to neutral, and the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 10min of 7000r/min, supernatant, the water that the 4.5kg precipitation adds 180kg again repeats the complex enzyme treatment step, the supernatant of centrifugation merges stand-by.With the centrifuged supernatant of the 330L of above-mentioned gained, 60 ℃ are evaporated to 165L; Handle pillar volume 5000ml, flow velocity 500ml/min with Subacidity cation exchange columns in series then.The concentrate of collecting is carried out spray-drying, and outlet temperature is 150 ℃, and air mass flow is 150m
3/ h promptly obtains beche-de-mer ovum nurture after the drying.Product is the faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
Claims (7)
1. beche-de-mer ovum nurture is characterized in that being is raw material by sea cucumber ovum, intestines or its mixture, makes through fragmentation, enzymolysis, ion-exchange, freeze drying; This nutriment is white or faint yellow solid powder, odorless, tasteless, and soluble in water and ethanol has hygroscopicity, polyoses content 〉=0.1wt%; Content of peptides 〉=8.0wt%; Moisture≤6.0wt%; Lead≤2.0mg/kg; Cadmium≤0.1mg/kg; Arsenic≤0.5mg/kg; Mercury≤0.3mg/kg; Total number of bacteria≤1000/ml, coliform group count≤90/100ml, pathogenic bacteria must not detect.
2. the preparation method of beche-de-mer ovum nurture according to claim 1 is characterized in that processing step is:
(1) raw material is handled: the sea cucumber ovum is separated with the sea cucumber body wall with intestines, through clean and remove silt, impurity after its fragmentation is standby;
(2) enzymolysis: the water that sea cucumber ovum after will handling according to the order of sequence and intestines add 10～100 times of its quality mixes, transferring pH is 3～8, place 30～50 ℃ of water-baths to handle 2～8h through the endogenous enzymes self-dissolving, the pepsin that adds solution quality 0.1～2.0% then adopts continous way to stir enzymolysis 2～5h down at 30～70 ℃; Keep above-mentioned pH value during hydrolysis, hydrolysis finishes and transfers pH to neutral, then through high temperature go out enzyme, after being chilled to room temperature, the centrifugal 10～30min of 3000～8000r/min supernatant, precipitation adds water again and repeats the pepsin step for 10～50 times, the supernatant of centrifugation merges;
(3) concentrate: centrifuged supernatant is evaporated to 1/2～1/10 of original volume in 20～60 ℃;
(4) remove metal ion: remove metal ion in the concentrate, flow velocity 100～1000ml/min with faintly acid or alkalescent cation exchange columns in series;
(5) drying: concentrate is carried out freeze drying under the following conditions: regulating solidification point is 20～40 ℃, and condenser temperature is-20～-40 ℃, and vacuum is 5～30Pa, to moisture be 1～6wt%.
3. as the preparation method of beche-de-mer ovum nurture as described in the claim 2, it is characterized in that processing step (2) enzymolysis is: the water that sea cucumber ovum after treatment and intestines add 10～100 times of its quality mixes, transferring pH is 3～8, the pepsin that adds solution quality 0.5～3.0%, adopt continous way to stir enzymolysis 3～8h down at 30～70 ℃, keep above-mentioned pH value during hydrolysis; Hydrolysis finishes and transfers pH to neutral, and the high temperature enzyme that goes out is chilled to room temperature, the centrifugal 10～30min of 3000～8000r/min, supernatant, precipitation adds water again and repeats above-mentioned steps 1～2 time for 10～50 times, the supernatant of centrifugation merges.
4. as the preparation method of beche-de-mer ovum nurture as described in the claim 2, it is characterized in that processing step (2) enzymolysis for to carry out mixed enzymolysis with trypsase, neutral proteinase and alkali protease, the mass ratio of trypsase, neutral proteinase and alkali protease is 1:1～3:1～3.
5. as the preparation method of beche-de-mer ovum nurture as described in the claim 2, it is characterized in that processing step (5) drying steps adopts spray-drying, its outlet temperature is 100～250 ℃, and air mass flow is 50～300m
3/ h.
6. as the preparation method of beche-de-mer ovum nurture as described in the claim 2,3 or 4, it is characterized in that described raw material is the sea cucumber ovum.
7. as the preparation method of beche-de-mer ovum nurture as described in the claim 2,3 or 4, it is characterized in that described raw material is a sea cucumber intestine.
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| CN2008100134265A CN101361578B (en) | 2008-09-25 | 2008-09-25 | Beche-de-mer ovum nurture and preparation method thereof |
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| CN2008100134265A CN101361578B (en) | 2008-09-25 | 2008-09-25 | Beche-de-mer ovum nurture and preparation method thereof |
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| CN101361578A true CN101361578A (en) | 2009-02-11 |
| CN101361578B CN101361578B (en) | 2011-05-11 |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664210B (en) * | 2009-09-29 | 2012-05-23 | 山东省海洋水产研究所 | Processing method of sea cucumber egg nutrition |
| CN102605031A (en) * | 2012-04-10 | 2012-07-25 | 武汉普赛特膜技术循环利用有限公司 | Method for comprehensively using viscera of sea cucumber |
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| CN1207990C (en) * | 2002-08-30 | 2005-06-29 | 大连轻工业学院 | Food rich in seacucumbus mucopolysaccharide and its preparation method |
| CN1202751C (en) * | 2002-08-30 | 2005-05-25 | 大连轻工业学院 | Seacucumbus whole powder food and its preparation method |
| JP2007153768A (en) * | 2005-12-02 | 2007-06-21 | Fuji Sangyo Kk | Immunoameliorator and method for producing the same |
| CN1994107A (en) * | 2005-12-31 | 2007-07-11 | 大连玉璘企业集团有限公司 | Method for preparing polypeptide nutrient |
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| CN101664210B (en) * | 2009-09-29 | 2012-05-23 | 山东省海洋水产研究所 | Processing method of sea cucumber egg nutrition |
| CN101756248B (en) * | 2009-10-30 | 2013-07-10 | 嵊泗海洋之星生物科技发展有限公司 | Preparation method of sea cucumber polypeptide capsule |
| WO2013091301A1 (en) * | 2011-12-22 | 2013-06-27 | 大连工业大学 | Method of use of sea cucumber intestine autolysis for preparing active substance |
| JP2014504160A (en) * | 2011-12-22 | 2014-02-20 | 大連工業大学 | Production method of active material by self-digestion of sea cucumber intestine |
| CN102605031A (en) * | 2012-04-10 | 2012-07-25 | 武汉普赛特膜技术循环利用有限公司 | Method for comprehensively using viscera of sea cucumber |
| CN103120328A (en) * | 2013-01-22 | 2013-05-29 | 浙江大学舟山海洋研究中心 | Preparation method of antioxidative peptide of acaudina molpadioides |
| CN103610065A (en) * | 2013-12-11 | 2014-03-05 | 威海力元海洋生物科技有限公司 | Sea cucumber and maca composition and preparation method thereof |
| CN105325801B (en) * | 2014-08-15 | 2020-05-22 | 中国海洋大学 | Preparation method of sea cucumber intestinal peptide |
| CN105325801A (en) * | 2014-08-15 | 2016-02-17 | 中国海洋大学 | Preparation method of sea cucumber intestinal peptide |
| CN106889295A (en) * | 2017-02-09 | 2017-06-27 | 赵德润 | A kind of preparation method of live body sea stichopus japonicus active peptide |
| CN107668570A (en) * | 2017-11-06 | 2018-02-09 | 刘海涛 | A kind of preparation method of sea cucumber pulvis, sea cucumber tablet and sea cucumber capsule |
| CN108514084A (en) * | 2018-03-14 | 2018-09-11 | 大连工业大学 | A method of based on biological enzyme tenderizing sea cucumber |
| CN110279108A (en) * | 2019-06-27 | 2019-09-27 | 烟台东宇海珍品有限公司 | A kind of preparation method for the sea cucumber product that human body easily absorbs |
| CN114128880A (en) * | 2021-11-08 | 2022-03-04 | 大连海宝生物技术有限公司 | Female sea cucumber egg nutrient solution and preparation method thereof |
| CN114934090A (en) * | 2022-06-29 | 2022-08-23 | 北海黑珍珠海洋生物科技有限公司 | Sea cucumber peptide with moisturizing effect and preparation method thereof |
| CN114934090B (en) * | 2022-06-29 | 2023-11-24 | 北海黑珍珠海洋生物科技有限公司 | Sea cucumber peptide with moisturizing effect and preparation method thereof |
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