CN105565910A - Preparation method of bioactive polypeptide calcium fertilizer - Google Patents
Preparation method of bioactive polypeptide calcium fertilizer Download PDFInfo
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- CN105565910A CN105565910A CN201510944758.5A CN201510944758A CN105565910A CN 105565910 A CN105565910 A CN 105565910A CN 201510944758 A CN201510944758 A CN 201510944758A CN 105565910 A CN105565910 A CN 105565910A
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- calcium
- fertilizer
- preparation
- biologically active
- bioactive polypeptide
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a preparation method of a bioactive polypeptide calcium fertilizer, belonging to the field of fertilizer preparation. The preparation method comprises the following steps: adding certain amounts of calcium oxide and manganese oxide into a polysuccinimide suspension, and carrying out hydrolysis reaction to obtain a bioactive polypeptide calcium suspension; adding acid into the polypeptide calcium suspension to regulate the pH value to 5-8, thereby obtaining a bioactive polypeptide calcium clear solution; and adding the bioactive polypeptide calcium clear solution into a fertilizer to obtain the bioactive polypeptide calcium fertilizer. According to the preparation method, the calcium oxide used as a calcium source contacts water in the water solution system to release heat, thereby being beneficial to increase of the reaction rate; and by introducing manganese ions into the polypeptide calcium fertilizer, the calcium and manganese ions are chelated by the peptide bond and carboxyl group on the main chain to generate a stable five-element ring, thereby endowing the polypeptide calcium with bioactivity, greatly postponing the aging of plants due to the bioactivity on the premise of supplementing calcium to the plants, and having obvious yield increase effect.
Description
Technical field
The invention belongs to fertilizer preparation field, be specifically related to a kind of preparation method of biologically active polypeptides calcareous fertilisers.
Background technology
Along with the development of reform and opening-up, agricultural, under various chemical fertilizer (urea, composite fertilizer etc.) uses in a large number, obtains continuous print high yield for many years, ensure that the stability of China's grain.But along with the high yield year after year of agricultural, in soil, various metallic element is consumed in a large number, causes the degeneration of soil fertility, especially the disappearance of calcium, the safety of serious threat China grain.
On Vehicles Collected from Market, agricultural is replenished the calcium with calcium hydroxide, and the inorganic calciums such as calcium oxide are main.Although inorganic calcium is cheap, its weak effect, plant difficulty absorbs, and long-term application, cause soil pH to raise.A large amount of inorganic calciums is with soil erosion, and enrichment in rivers and lakes, pollutes.Market is badly in need of a kind of plant easily to absorb, effective, the not novel calcareous fertilisers-biologically actived calcium of contaminated soil.
Biologically actived calcium refers to biogenic organic calcium, pure natural but not synthetic or processing, have particle little, absorb fast, safety, without side reaction, be beneficial to people and animals and plants absorption.But be limited to raw material sources, biologically actived calcium always cannot large-scale industrial production.Seriously limit it to use.
Possess bioactive polypeptide calcium, refer to that main chain is polypeptide, possess the characteristic of biologically actived calcium, and its source is obtained by synthetic.
In existing patent, possess bioactive polypeptide calcium Patents there are no synthetic, only see the patent having synthesizing polyaspartic acid calcium.As:
Chinese patent ZL200610091160.7 discloses a kind of preparation method of calcium polyaspartate: with poly aspartic acid acid for raw material, add calcium oxide or calcium hydroxide, then through alcohol settling, heat filtering separating-purifying obtains calcium polyaspartate.When it is prepared, adopt excessive calcium oxide and calcium hydroxide, the quality product that have impact on, and cause difficulty to subsequent purification; Adopt alcohol settling to carry out separating-purifying calcium polyaspartate, consume a large amount of organic solvent (ethanol), not only increase cost, and easily cause environmental pollution; Prepared poly-sky calcium does not possess biological activity, and agricultural produce effect is not remarkable.
Chinese patent ZL201110326607.5 discloses a kind of preparation method of agricultural calsium supplement calcium polyaspartate.In its patent, calcium used is calcium hydroxide, and the carbonic acid gas of calcium hydroxide easily and in air generates precipitation of calcium carbonate, not easily preserves; Prepared product does not possess biological activity, and effect of increasing production is not remarkable.
Summary of the invention
The object of this invention is to provide a kind of preparation method of biologically active polypeptides calcareous fertilisers, its concise production process, cost is low, environmental friendliness, no coupling product produces, and products obtained therefrom biological activity is high, while replenishing the calcium, delay plant senescence, possess significant effect of increasing production, various soil can be applied to.
To achieve these goals, the technical solution used in the present invention is:
The preparation method of biologically active polypeptides calcareous fertilisers of the present invention, comprises the following steps:
A, join in the suspension of polysuccinimide by quantitative calcium oxide and manganese oxide, be hydrolyzed reaction, obtains possessing bioactive polypeptide calcium suspension liquid;
B, to acid adding in above-mentioned polypeptide calcium suspension liquid, be adjusted to pH=5-8, obtain possessing bioactive polypeptide calcium clear liquor;
C, aforementioned polypeptides calcium clear liquor is joined in fertilizer, obtain biologically active polypeptides calcareous fertilisers.
In step a, the consumption mol ratio of calcium oxide and polysuccinimide is 0.55:1.
In step a, the consumption of calcium oxide and manganese oxide is 100:(1-5).
In step b, acid used is any one in nitric acid, formic acid, acetic acid or phosphoric acid, and its consumption is to regulate pH for till 5-8.
In step c, in fertilizer, the addition of polypeptide calcium clear liquor is 3 ‰-3%.
Described fertilizer is composite fertilizer, urea, any one in foliage fertilizer, Water soluble fertilizer, bio-bacterial manure, fertilizer or Chemical Mixed Fertilizer.
Beneficial effect of the present invention is: the preparation method of biologically active polypeptides calcareous fertilisers of the present invention, adopt calcium oxide to be calcium source, in water solution system, water met by calcium oxide, release of heat, contribute to accelerating speed of reaction, solve the problem that the carbonic acid gas of calcium hydroxide easily and in air generates precipitation of calcium carbonate; Polypeptide calcareous fertilisers introduce mn ion, and calcium and mn ion, by the peptide bond on main chain and carboxyl chelating, generate stable five-ring, give polypeptide calcium biological activity, while supplementing calcium to plant, its biological activity will delay the aging of plant greatly, plays significant effect of increasing production.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further details:
The polysuccinimide adopted in this enforcement, calcium oxide, manganese oxide, acid are technical grade.
Embodiment 1
The preparation method of biologically active polypeptides calcareous fertilisers, comprises the following steps:
A, by the calcium oxide of 62kg and the manganese oxide of 0.8kg, in the disposable aqeous suspension joined containing 217kg polysuccinimide and 720kg, stirring at room temperature 6h, obtains possessing bioactive polypeptide calcium suspension liquid;
The nitric acid of b, use 2kg60%, by aforementioned polypeptides calcium suspension liquid, is adjusted to pH=6.2, obtains possessing bioactive polypeptide calcium clear liquor;
C, join in composite fertilizer by aforementioned polypeptides calcium clear liquor, addition is 3 ‰, obtains biologically active polypeptides calcium, composite fertilizer.
Embodiment 2
The preparation method of biologically active polypeptides calcareous fertilisers, comprises the following steps:
A, by the calcium oxide of 65kg and the manganese oxide of 0.8kg, in the disposable aqeous suspension joined containing 217kg polysuccinimide and 720kg, stirring at room temperature 6h, obtains possessing bioactive polypeptide calcium suspension liquid;
The acetic acid of b, use 4kg60%, by aforementioned polypeptides calcium suspension liquid, is adjusted to pH=5, obtains possessing bioactive polypeptide calcium clear liquor;
C, to be added by aforementioned polypeptides calcium clear liquor and join in the melting pipeline of urea, addition is 3%, and be mixed together evenly with urine slurry, granulation, obtains biologically actived calcium urea fertilizer.
Embodiment 3
The preparation method of biologically active polypeptides calcareous fertilisers, comprises the following steps:
A, by the calcium oxide of 62kg and the manganese oxide of 2.4kg, in the disposable aqeous suspension joined containing 217kg polysuccinimide and 720kg, stirring at room temperature 6h, obtains possessing bioactive polypeptide calcium suspension liquid;
The phosphoric acid of b, use 3kg60%, by aforementioned polypeptides calcium suspension liquid, is adjusted to pH=8, obtains possessing bioactive polypeptide calcium clear liquor;
C, to be added by aforementioned polypeptides calcium clear liquor and join in Water soluble fertilizer, addition is 3%, obtains biologically actived calcium Water soluble fertilizer.
Measure the biological activity of the biologically active polypeptides calcium clear liquor that step b in embodiment 1-3 obtains, instrument is UV-4802 type temperature controllable spectrophotometer.
Measure its result suppressing ultra-oxygen anion free radical disproportionation reaction according to the concentration 3-30 agriculturally applied (mg/kg), calculate inhibiting rate and the vigor of biologically active polypeptides calcium, refer to table 1.
Table 1
Note: be take 3 parts all simultaneously carry out replicate(determination) to measuring sample each time in upper table, getting arithmetic average is result.
Table 2 is Rate activity and the natural SOD of biologically actived calcium and the ratio of common calcium polyaspartate.
| Sample | Rate activity (U/mg) | The Rate activity (%) of biologically actived calcium/natural SOD |
| Natural SOD | 4654.2 | —————— |
| Calcium polyaspartate | 0 | 0 |
| Embodiment 1 | 2366.5 | 50.8 |
| Embodiment 2 | 2382.9 | 51.2 |
| Embodiment 3 | 2952.1 | 63.4 |
Table 2
Note: the SOD amount that the vigor in table 2 reaches corresponding to 50% for: every milligram of tissue protein or stand-in SOD inhibiting rate in 1mL reaction solution is a SOD vigor (U/mgprot).
Claims (6)
1. a preparation method for biologically active polypeptides calcareous fertilisers, is characterized in that, comprises the following steps:
A, join in the suspension of polysuccinimide by quantitative calcium oxide and manganese oxide, be hydrolyzed reaction, obtains possessing bioactive polypeptide calcium suspension liquid;
B, to acid adding in above-mentioned polypeptide calcium suspension liquid, be adjusted to pH=5-8, obtain possessing bioactive polypeptide calcium clear liquor;
C, aforementioned polypeptides calcium clear liquor is joined in fertilizer, obtain biologically active polypeptides calcareous fertilisers.
2. the preparation method of biologically active polypeptides calcareous fertilisers according to claim 1, is characterized in that, in step a, the consumption mol ratio of calcium oxide and polysuccinimide is 0.55:1.
3. the preparation method of biologically active polypeptides calcareous fertilisers according to claim 1, is characterized in that, in step a, the consumption of calcium oxide and manganese oxide is 100:(1-5).
4. the preparation method of biologically active polypeptides calcareous fertilisers according to claim 1, is characterized in that, in step b, acid used is any one in nitric acid, formic acid, acetic acid or phosphoric acid, and its consumption is to regulate pH for till 5-8.
5. the preparation method of biologically active polypeptides calcareous fertilisers according to claim 1, is characterized in that, in step c, in fertilizer, the addition of polypeptide calcium clear liquor is 3 ‰-3%.
6. the preparation method of biologically active polypeptides calcareous fertilisers according to claim 1 or 5, it is characterized in that, described fertilizer is composite fertilizer, urea, any one in foliage fertilizer, Water soluble fertilizer, bio-bacterial manure, fertilizer or Chemical Mixed Fertilizer.
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| CN201510944758.5A CN105565910B (en) | 2015-12-16 | 2015-12-16 | The preparation method of biologically active polypeptide calcareous fertilisers |
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| CN201510944758.5A CN105565910B (en) | 2015-12-16 | 2015-12-16 | The preparation method of biologically active polypeptide calcareous fertilisers |
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| CN105565910A true CN105565910A (en) | 2016-05-11 |
| CN105565910B CN105565910B (en) | 2017-03-08 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106465627A (en) * | 2016-08-31 | 2017-03-01 | 河北省农林科学院石家庄果树研究所 | A kind of cultural method of high yield Fructus Mali pumilae |
| CN108911832A (en) * | 2018-04-28 | 2018-11-30 | 金华市景和科技有限公司 | The compounding agent of rice calcium content can be improved |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465697A (en) * | 2002-06-29 | 2004-01-07 | 北京联合大学职业技术师范学院 | Polycondensed amino-acid coordinated SOD simulatives |
| CN101100510A (en) * | 2006-07-04 | 2008-01-09 | 北京联合大学 | Calcium polyaspartate |
| CN101305810A (en) * | 2008-07-14 | 2008-11-19 | 李绍通 | Polypeptide calcium and use |
| CN102504253A (en) * | 2011-10-25 | 2012-06-20 | 石家庄开发区德赛化工有限公司 | Environmental-protection preparation method for calcium polyaspartate as agricultural calcium supplement |
-
2015
- 2015-12-16 CN CN201510944758.5A patent/CN105565910B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1465697A (en) * | 2002-06-29 | 2004-01-07 | 北京联合大学职业技术师范学院 | Polycondensed amino-acid coordinated SOD simulatives |
| CN101100510A (en) * | 2006-07-04 | 2008-01-09 | 北京联合大学 | Calcium polyaspartate |
| CN101305810A (en) * | 2008-07-14 | 2008-11-19 | 李绍通 | Polypeptide calcium and use |
| CN102504253A (en) * | 2011-10-25 | 2012-06-20 | 石家庄开发区德赛化工有限公司 | Environmental-protection preparation method for calcium polyaspartate as agricultural calcium supplement |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106465627A (en) * | 2016-08-31 | 2017-03-01 | 河北省农林科学院石家庄果树研究所 | A kind of cultural method of high yield Fructus Mali pumilae |
| CN108911832A (en) * | 2018-04-28 | 2018-11-30 | 金华市景和科技有限公司 | The compounding agent of rice calcium content can be improved |
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| CN105565910B (en) | 2017-03-08 |
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Address after: 272400 Jiaxiang County Economic Development Zone, Jining, Shandong, south of Xinmin Road, south of the West Road (80 meters west of Qingshan scenic spot) Patentee after: SHANDONG YUANLIAN CHEMICAL CO.,LTD. Address before: 272400 Zhongshan Industrial Park, Zhongshan town, Jiaxiang, Jining, Shandong, China Patentee before: SHANDONG YUAN LIAN CHEMICAL Co.,Ltd. |
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Denomination of invention: Preparation of bioactive polypeptide calcium fertilizer Effective date of registration: 20200820 Granted publication date: 20170308 Pledgee: Shandong Jiaxiang Rural Commercial Bank Co.,Ltd. Pledgor: SHANDONG YUANLIAN CHEMICAL Co.,Ltd. Registration number: Y2020980005170 |
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Date of cancellation: 20220727 Granted publication date: 20170308 Pledgee: Shandong Jiaxiang Rural Commercial Bank Co.,Ltd. Pledgor: SHANDONG YUANLIAN CHEMICAL CO.,LTD. Registration number: Y2020980005170 |