CN103044147B - Fertilizer effect enhancing additive and application thereof - Google Patents
Fertilizer effect enhancing additive and application thereof Download PDFInfo
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- CN103044147B CN103044147B CN 201310014107 CN201310014107A CN103044147B CN 103044147 B CN103044147 B CN 103044147B CN 201310014107 CN201310014107 CN 201310014107 CN 201310014107 A CN201310014107 A CN 201310014107A CN 103044147 B CN103044147 B CN 103044147B
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Abstract
The invention relates to a fertilizer effect enhancing additive. The additive mainly comprises 2, 4-dinitrophenol, alpha-naphthyl acetic acid, methoxy-5-nitrophenol, 2-nitrophenol, p-nitrophenol, dodecylbenzene sulfonic acid, polyaspartic acid, triiodobenzoic acid, biological azotobacters, protein peptides and polyether ternary alcohol. According to the additive provided by the invention, the effect of fertilizer is enhanced effectively and the utilization rate of the fertilizer is improved, so that the purposes of increasing the yield and lowering the fertilizer consumption are realized. Theadditive also has the advantages that the environmental pollution is reduced, and the yield and the quality of crops, flowers, mushrooms and vegetables are improved.
Description
Technical field
The present invention relates to the additive agent field of agrochemical, be specifically related to a kind of fertilizer synergism additive and uses thereof.
Background technology
Along with the growth of world population, industrial expansion and city size constantly enlarge, and a large amount of arable lands is occupied, and cultivated area is reduced year by year.In order to satisfy people to the demand of grain and agricultural byproducts, must increase the output of grain, be the effective means that improves grain yield and apply chemical fertilizer.At present traditional fertilizer is such as nitrate fertilizer, ammoniacal nitrogen fertilizer, amide nitrogen fertilizer, cyanamide nitrogen fertilizer etc., certain effect of increasing production is all arranged, because these chemical fertilizer volatilizations are fast, utilization ratio is low, wanting to obtain volume increase must execute more, this is invisible to have increased burden to the peasant, simultaneously a large amount of life-time service also bring many side effects, as: to environment, the infiltration by rainwater all produces in various degree pollution to underground water and rivers and lakes.
The use of fertilizer makes agriculture production obtain huge progress, can make Limited Land offer to human more results.In recent decades, the production of conventional fertilizers and use have reached historical summit, and people find that more and more the simple increasing rate of fertilizer application that relies on can not obtain higher output.Fertilizer brands numerous on the market do not have too large difference in essence.Product homogeneity is day by day fierce, and the producer of fertilizer and user are expecting that the development of current science and technology can bring huge variation to fertilizer industry.
Summary of the invention
In order to overcome the existing low deficiency of chemical fertilizer utilization ratio, solve the problem that life-time service can cause environmental pollution, the invention provides a kind of fertilizer efficiency that can strengthen chemical fertilizer, improve chemical fertilizer utilization ratio, thereby reach the purpose fertilizer additive that improves output and use less chemical fertilizer, this additive reduces the pollution to environment simultaneously, and improves farm crop, flowers, mushroom and vegetable yields and quality.
The present invention adopts following technical scheme to realize goal of the invention of the present invention:
A kind of fertilizer synergism additive, its weight ratio prescription is as follows: 2,2, 4-dinitrophenol or its sodium salt 0.1-60 part, α-naphthaleneacetic acid or its sodium salt 0.1-40 part, 5 nitroguaiacol or its sodium salt 0.1-20 part, adjacent nitro tea phenol or its sodium salt 0.1-30 part, p-NP or its sodium salt 0.1-30 part, Witco 1298 Soft Acid or its sodium salt 0.1-10 part, poly aspartic acid 0.1-20 part, Triiodobenzoic acid 0.01-0.1 part, biological nitrogen fixation bacterium 1-6 part, protein peptide 1-6 part, polyether-tribasic alcohol 4-5 part, wherein said protein peptide is protein peptide A, B, C.
The present invention is preferably: according to fertilizer synergism additive described above, its weight ratio prescription is as follows:
2,20 parts of 2, 4-dinitrophenol or its sodium salts, 10 parts of α-naphthaleneacetic acid or its sodium salts, 10 parts of 5 nitroguaiacol or its sodium salts, 15 parts of adjacent nitro tea phenol or its sodium salts, 15 parts of p-NP or its sodium salts, 5 parts of Witco 1298 Soft Acid or its sodium salts, 10 parts of poly aspartic acids, 0.05 part of Triiodobenzoic acid, 3 parts of biological nitrogen fixation bacterium, 6 parts of protein peptide, 4 parts of polyether-tribasic alcohols, wherein said biological nitrogen fixation bacterium is preferably Wei Nielande vinelandii (Azotobacter vinelandii) and blown-ball Azotobacter (Azotobacter chroococcum), two kinds of bacterium preferably prepare microbial inoculum according to the ratio of 1:1, described protein peptide A, B, C prepares according to 1:1:1, and the aminoacid sequence of wherein said protein peptide A is shown in SEQ ID NO:1, the aminoacid sequence of protein peptide B is shown in SEQ ID NO:2, the aminoacid sequence of protein peptide C shown in SEQ ID NO:3, protein peptide A, B is respectively magnetic Nano protein peptide A, slowly-releasing protein peptide B.
The invention still further relates to a kind of using method of fertilizer synergism additive, it is characterized by fertilizer synergism additive of the present invention is mixed with the urea raw material in the ratio of the 0.01-5% of urea raw material weight, be processed into peptide urea composite fertilizer, its mixture is processed into particulate state, then is administered to crop or plant.
Further, the using method of fertilizer synergism additive can also be: the water mixed-shaped yellowly mashed prod that aforesaid fertilizer synergism additive is doubly measured with the 1-5 of its weight first in 1% ratio of urea raw material weight.With yellow mashed prod and urea raw material stirring mixing, its mixture is processed into particulate state, then be administered to crop or plant.
The invention still further relates to the purposes of a kind of fertilizer synergism additive aspect the various farm crop of raising, flowers, mushroom and vegetable yields and quality.Wherein, preferably in the purposes that improves aspect tomato, corn, cotton, capsicum, Chinese cabbage, melon and fruit, red date, mushroom, the flowers yield and quality.
2,4-DNP (sodium): for light yellow microcrystal, soluble in water, have very strong water-absorbent, the performances such as emulsification, dispersion can promote cell fission and tissue differentiation, hestening rooting is used for that fault is expanded and the expanding of piece root stem tuber, and induces the formation of root and adventive root.
α-naphthaleneacetic acid (sodium): the broad spectrum plant-growth regulator, promote cell fission and tissue differentiation, hestening rooting is used for expanding of Fruit and piece root stem tuber.Induce the formation of root and adventive root, function is taken root and the seed root of hair in accelerating the cuttage branch, also can be used for flower and fruit protecting, improves output, strengthens anti-adversity ability etc.
5 nitroguaiacol (sodium): but flower and fruit protecting improves fruit-setting rate; Promote root growth, increase female flower, increase piece root output and sugar degree.
O-NP (sodium); Acidity, soluble in water, have very strong water-absorbent, the performances such as emulsification, dispersion.Be the vegetable cell activating agent, can be used for Promoting plant growth and grow, early flowering, breaking dormancy, stratification prevents fruit drop, the aspects such as quality of improvement plant prod.
P-NP (sodium): be the vegetable cell activating agent, its energy rapid permeability is in plant materials, to promote cellular plasm to flow, accelerate plant establishment speed, the etap such as plant rooting, growth, reproduction and result all there is in various degree promoter action.Especially for the promotion of the elongation of pollen tube, help the effect of fertilization particularly evident, can be used for Promoting plant growth growth, early flowering, breaking dormancy, stratification, prevent fruit drop, the problems such as quality of improvement plant prod.
Sodium dodecylbenzene sulfonate: white is to little yellow powdery or particle, and is nontoxic, tasteless, and a small amount of stimulation is arranged, and can be dissolved in water, has emulsion dispersion effect, antistatic property etc.; The anti-moisture absorption of the powdery Chemicals of inorganic, organic easy moisture absorptions such as urotropine, urea, ammonium nitrate, ammonium chloride, caking, caking additive.Add about 0.3～0.5%, can play the increase crystalline particle, stop the moisture absorption, eliminate static, thereby prevent the stabilising effect that lumps, this product is the efficient additive that improves the quality of chemical products of inorganic, organic easy moisture absorption, caking.
Poly aspartic acid PASP can make the nutrition absorption toughener on agricultural, promote the growth of the farm crop such as melon and fruit, vegetables, flowers, adds with fertilizer simultaneously and can improve fertilizer efficiency.
Triiodobenzoic acid: a kind of auxin substance of synthetic, can promote transplants takes root and promotes solid.
Biological bacteria: vinelandii can increase crop yield, its specificity is not strong, be widely used, the present invention adopts blown-ball Azotobacter and two kinds of vinelandii mixing of Wei Nielande vinelandii to carry out biological nitrogen fixation, and the effect that increases crop yield can be effectively played in the mixing of these two kinds of vinelandii.The preferred biological nitrogen fixation bacterium of the present invention is Wei Nielande vinelandii (Azotobacter vinelandii) and blown-ball Azotobacter (Azotobacter chroococcum), can buy by the commercial channel to obtain.Adopt the production technology of compound bacterial fertilizer to be prepared, concrete grammar is as follows:
Two kinds of vinelandii are carried out respectively actication of culture, be inoculated on the slant medium, 25-30 ℃, cultivate 30-38h, pH6.5-7.2 produces first class inoculum; Get first class inoculum, be inoculated in the liquid medium, through 28-30 ℃, 24-40 hour cultivation, its nutrient solution is second class inoculum, obtains three-class strain through enlarged culturing again; Being prepared as of liquid nutrient medium: peptone 20.0g, glucose 15.0g, yeast extract paste 7.0g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, tween 80 1mL, magnesium sulfate heptahydrate 0.58g, four water manganous sulfate 0.25g, distilled water 1000mL, adjust pH is 6.8～7.0, and sterilising treatment can be carried out further enlarged culturing as required.Two kinds of bacterium of Wei Nielande vinelandii and blown-ball Azotobacter prepare microbial inoculum according to the ratio of 1:1-1:2.Detect, check and accept finally by quality product.
Protein peptide: the aminoacid sequence of wherein said protein peptide A is shown in SEQ ID NO:1, and the aminoacid sequence of protein peptide B is shown in SEQ ID NO:2, and the aminoacid sequence of protein peptide C is shown in SEQ ID NO:3.Protein peptide A of the present invention, B, C can satisfy the demand of biological growth, promote the growth of crop and vegetables.Wherein optimization protein peptide A is the magnetic microsphere protein peptide with adsorptive power, and protein peptide B is the protein peptide with long-acting slow-release effect, and effective use of magnetic microsphere protein peptide A, slowly-releasing protein peptide B and protein peptide C more can promote the raising of crop and vegetable crop.
In the specific embodiment of the present invention, the preparation method is: add the sodium hydroxide solution 100ml of 5mol/L in flask, stir logical nitrogen in 69 ℃ of speed with 120r/min and remove water oxygen gas, add 15ml0.6mol/LFeSO behind the 45min
4Solution and 45ml0.5mol/LFeCl
3Solution, stir 2 hours with 350r/min after, stopped heating, whole reaction is carried out under nitrogen protection, reactant magnetic resolution and with distilled water wash for several times.Get 10ml (25mg/mL) nanoscale magnetic bead with the ethanol cleaning and be dissolved in the 100ml ethanol rapid stirring 2 hours, adding ammoniacal liquor 10ml in warm water bath, distilled water 8ml, vigorous stirring 4 hours, ultrasonic 40 minutes, ethanol is washed 3 times, again is dissolved in the ethanol, obtains nano-magnetic microsphere.Fe with preparation
3O
4/ SiO
2After being scattered in ethanol and regulating concentration ultrasonic 20 minutes, add the APTES silylating reagent and carry out amination.
In the specific embodiment of the present invention, the nucleotide coding sequence of synthetic above-mentioned protein peptide A, B, C, genetic engineering technique according to this area routine, above-mentioned encoding sequence is connected to suitable carrier, in host cell, express and purifying, the preferred pichia pastoris phaff GS115 of host cell, carrier for expression of eukaryon is pPIC9.Expression vector pPIC9-A, the pPIC9-B, the pPIC9-C that build are used respectively the BglII linearization for enzyme restriction, adopting electricity to turn method changes goal gene among the yeast GS115 over to and expresses, yeast liquid after transforming is coated on the selection substratum MD of HIS disappearance, cultivate for 28 ℃ and grew yeast mono-clonal bacterium colony in 3 days, choose respectively again to MM and the contrast of MD substratum, identify phenotype.PCR to the pichia pastoris phaff transformant identifies, after employing HIS selects the substratum preliminary evaluation, carries out PCR and detects, and the result shows that all yeast transformants are positive findings, and the illustration purpose gene successfully changes among the yeast GS115.Recombinant bacterial strain GS115/pPIC9-A, GS115/pPIC9-B, GS115/pPIC9-C are inoculated in the conventional medium, press the inoculation of 5%-10% inoculum size, cultivate 36 hours centrifugal receipts bacterium for 30 ℃, the access conventional medium, methanol induction is expressed, cultivated 24-72 hour for 30 ℃, centrifugal collection fermented supernatant fluid, through DEAE-Sepharose FF ion exchange column, carry out the salt gradient wash-out with this damping fluid that contains 0-1mol/LNaCL, flow velocity is 0.1-3ml/min, collects the Peak Activity liquid at the 2nd, 3,4 peaks, concentrated, dry, obtain respectively protein peptide A, B, C.Empirical tests, aminoacid sequence is correct.
In the specific embodiment of the present invention, the protein peptide A that obtains is dissolved among the 50%ACN-0.1%TFA, add Fe
3O
4/ SiO
2Nano material at room temperature behind the vortex 20min, is put into magnet in the bottom of centrifuge tube, and centrifugal 150r/min removes supernatant liquor, repeats twice, uses 0.1MNH
3.H
2O wash-out Fe
3O
4/ SiO
2Nano material obtains magnetic Nano protein peptide A.
In the specific embodiment of the present invention, protein peptide B is ground into 200-300 purpose powder, it is stand-by that zeolite powder is broken into 150-260 order zeolite powder.Account for and add suitable quantity of water and stirring in the gross weight 10-20% stearic acid, the protein peptide B powder that will contain gross weight 12-15% adds wherein with stirring slowly, rotating speed 50-120rpm, stir 15-30min, add again and account for gross weight 15-25% zeolite powder, make suspension slurry, the centrifugal 5000-10000r/min of room temperature, 30-60min.Adjust slurries pH and reach 6.8-8.0, wash 3-5 time, filter or centrifuge dehydration, oven dry.Obtain having the slowly-releasing protein peptide B of slow releasing function.
With 2 of above-mentioned formula ratio, 2, 4-dinitrophenol or its sodium salt, α-naphthaleneacetic acid or its sodium salt, 5 nitroguaiacol or its sodium salt, adjacent nitro tea phenol or its sodium salt, p-NP or its sodium salt, Witco 1298 Soft Acid or its sodium salt, poly aspartic acid, Triiodobenzoic acid, the biological nitrogen fixation bacterium, protein peptide A, protein peptide B, protein peptide C mix, add and continue to stir 30min in the polyether-tribasic alcohol, drying forms the powdered fertilizer Synergistic additives.Wherein protein peptide A, B are preferably magnetic Nano protein peptide A, slowly-releasing protein peptide B.
The present invention has following beneficial effect:
1, overcomes the deficiency of existing chemical fertilizer, strengthen the fertilizer efficiency of chemical fertilizer, improve chemical fertilizer utilization ratio.
2, promote plant cell division and tissue differentiation, promote root growth, improve fruit-setting rate.
3, improve output, use chemical fertilizer less, reduced simultaneously the pollution to environment, improve the quality of farm crop, reaching increases production and improves productivity reduces the purpose of cost, and agricultural development is had meaning very far away.
Embodiment
Embodiment 1
Prescription: 0.15 part of 2,4-DNP, 0.4 part of α-naphthaleneacetic acid, 0.1 part of 5 nitroguaiacol, 0.1 part in adjacent nitro tea phenol, 0.29 part of p-NP, 0.2 part of Witco 1298 Soft Acid, 0.2 part of poly aspartic acid, 0.01 part of Triiodobenzoic acid, 5 parts of biological nitrogen fixation bacterium, 6 parts of protein peptide, 4 parts of polyether-tribasic alcohols, wherein the biological nitrogen fixation bacterium is selected Wei Nielande vinelandii and blown-ball Azotobacter, according to the ratio preparation of 1:1, each 2 parts of magnetic Nano protein peptide A, slowly-releasing protein peptide B, protein peptide C.
The preparation method of Wei Nielande vinelandii, blown-ball Azotobacter is: two kinds of vinelandii carried out respectively actication of culture, is inoculated on the slant medium, and 25 ℃, cultivate 30h, pH6.5 produces first class inoculum; Get first class inoculum, be inoculated in the liquid medium, cultivated through 28 ℃, 24 hours, its nutrient solution is second class inoculum, obtains three-class strain through enlarged culturing again; Being prepared as of liquid nutrient medium: peptone 20.0g, glucose 15.0g, yeast extract paste 7.0g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, tween 80 1mL, magnesium sulfate heptahydrate 0.58g, four water manganous sulfate 0.25g, distilled water 1000mL, adjust pH is 6.8, and sterilising treatment can be carried out further enlarged culturing as required.Above-mentioned two kinds of bacterium are prepared microbial inoculum according to the 1:1 ratio.Detect, check and accept finally by quality product.
With 2 of above-mentioned formula ratio, 2, 4-dinitrophenol, α-naphthaleneacetic acid, 5 nitroguaiacol, adjacent nitro tea phenol, p-NP, Witco 1298 Soft Acid, poly aspartic acid, Triiodobenzoic acid, the biological nitrogen fixation bacterium, magnetic Nano protein peptide A, slowly-releasing protein peptide B, protein peptide C mix, add and continue to stir 30min in the polyether-tribasic alcohol, drying forms the powdered fertilizer Synergistic additives.
Using method: the 0.01kg fertilizer synergism additive is mixed with the urea raw material of 100kg, be processed into peptide urea composite fertilizer, its mixture is processed into particulate state, use crop or plant.
Embodiment 2
Prescription: 60 parts of 2,4-DNPs, 40 parts of α-naphthaleneacetic acid, 20 parts of 5 nitroguaiacol, 30 parts in adjacent nitro tea phenol, 30 parts of p-NPs, 10 parts of Witco 1298 Soft Acids, 20 parts of poly aspartic acids, 0.1 part of Triiodobenzoic acid, 6 parts of biological nitrogen fixation bacterium, 6 parts of protein peptide, 5 parts of polyether-tribasic alcohols, wherein the biological nitrogen fixation bacterium is selected Wei Nielande vinelandii and blown-ball Azotobacter, according to the ratio preparation of 1:1, each 2 parts of magnetic Nano protein peptide A, slowly-releasing protein peptide B, protein peptide C.
The preparation method of Wei Nielande vinelandii, blown-ball Azotobacter is: two kinds of vinelandii carried out respectively actication of culture, is inoculated on the slant medium, and 30 ℃, cultivate 38h, pH7.2 produces first class inoculum; Get first class inoculum, be inoculated in the liquid medium, cultivated through 30 ℃, 40 hours, its nutrient solution is second class inoculum, obtains three-class strain through enlarged culturing again; Being prepared as of liquid nutrient medium: peptone 20.0g, glucose 15.0g, yeast extract paste 7.0g, dipotassium hydrogen phosphate 2g, dibasic ammonium citrate 2g, sodium acetate 5g, glucose 20g, tween 80 1mL, magnesium sulfate heptahydrate 0.58g, four water manganous sulfate 0.25g, distilled water 1000mL, adjust pH is 7.0, and sterilising treatment can be carried out further enlarged culturing as required.Above-mentioned two kinds of bacterium are prepared microbial inoculum according to the 1:1-1:2 ratio.Detect, check and accept finally by quality product.
With 2 of above-mentioned formula ratio, 2, 4-dinitrophenol, α-naphthaleneacetic acid, 5 nitroguaiacol, adjacent nitro tea phenol, p-NP, Witco 1298 Soft Acid, poly aspartic acid, Triiodobenzoic acid, the biological nitrogen fixation bacterium, magnetic Nano protein peptide A, slowly-releasing protein peptide B, protein peptide C mix, add and continue to stir 30min in the polyether-tribasic alcohol, drying forms the powdered fertilizer Synergistic additives.
Using method: the 0.5kg fertilizer synergism additive is mixed with the urea of 100kg, be processed into peptide urea composite fertilizer, its mixture is processed into particulate state, use crop or plant.
Embodiment 3
Prescription: 2,20 parts of 2, 4-dinitrophenols, 10 parts of α-naphthaleneacetic acid, 10 parts of 5 nitroguaiacol, 15 parts in adjacent nitro tea phenol, 15 parts of p-NPs, 5 parts of Witco 1298 Soft Acids, 10 parts of poly aspartic acids, 0.05 part of Triiodobenzoic acid, 3 parts of biological nitrogen fixation bacterium, 6 parts of protein peptide, 4 parts of polyether-tribasic alcohols, wherein Wei Nielande vinelandii and blown-ball Azotobacter prepare microbial inoculum according to the ratio of 1:1, and described protein peptide A, B, C are respectively 2 parts, 2 parts, 2 parts, 4 parts of polyether-tribasic alcohols, protein peptide A is magnetic Nano protein peptide A, and protein peptide B is slowly-releasing protein peptide B.
The preparation method of vinelandii is with embodiment 1.
With 2 of above-mentioned formula ratio, 2, 4-dinitrophenol, α-naphthaleneacetic acid, 5 nitroguaiacol, adjacent nitro tea phenol, p-NP, Witco 1298 Soft Acid, poly aspartic acid, Triiodobenzoic acid, the biological nitrogen fixation bacterium, magnetic Nano protein peptide A, slowly-releasing protein peptide B, protein peptide C mix, add and continue to stir 30min in the polyether-tribasic alcohol, drying forms the powdered fertilizer Synergistic additives.
Using method: the 1kg fertilizer synergism additive is mixed with the urea of 100kg, be processed into peptide urea composite fertilizer, its mixture is processed into particulate state, use crop or plant.
Embodiment 4
Prescription: 20 parts of 2,4-DNPs, 10 parts of α-naphthaleneacetic acid, 10 parts of 5 nitroguaiacol, 15 parts in adjacent nitro tea phenol, 15 parts of p-NPs, 5 parts of Witco 1298 Soft Acids, 10 parts of poly aspartic acids, 0.05 part of Triiodobenzoic acid, 3 parts of biological nitrogen fixation bacterium, 6 parts of protein peptide, 4 parts of polyether-tribasic alcohols, wherein Wei Nielande vinelandii and blown-ball Azotobacter prepare microbial inoculum according to the ratio of 1:1, described protein peptide A, B, C are respectively 2 parts, 2 parts, 2 parts, 4 parts of polyether-tribasic alcohols.
The preparation method of vinelandii is with embodiment 1.
With 2 of above-mentioned formula ratio, 2, 4-dinitrophenol, α-naphthaleneacetic acid, 5 nitroguaiacol, adjacent nitro tea phenol, p-NP, Witco 1298 Soft Acid, poly aspartic acid, Triiodobenzoic acid, the biological nitrogen fixation bacterium, protein peptide A, protein peptide B, protein peptide C mix, add and continue to stir 30min in the polyether-tribasic alcohol, drying forms the powdered fertilizer Synergistic additives.
Using method: the 1kg fertilizer synergism additive is mixed with the urea of 100kg, be processed into peptide urea composite fertilizer, its mixture is processed into particulate state, use crop or plant.
The manure trial of embodiment 5 peptide urea composite fertilizers of the present invention on tomato proterties and output
Test design:
(1) test is processed: in the consistent situation of base manure, 2 processing are established in test altogether, and processing A is habitual fertile amount; Processing C is the peptide urea composite fertilizer of embodiment 3 preparations, and usage quantity is 2/3 of habits fertilization amount.Adopt randomized block design, triplicate;
(2) fertilizer amount and application process
Process A: base manure: use the 15kg diammonium phosphate for every mu, 5kg urea and 3kg vitriolate of tartar; Topdress: the urea 15kg/ mu that topdresses for the first time flowering period, the fruit expanding period urea 15kg/ mu that topdresses for the second time.
Process C: base manure: use the 15kg diammonium phosphate for every mu, 5kg urea and 3kg vitriolate of tartar; Topdress: the peptide urea composite fertilizer 10kg/ mu that topdresses for the first time flowering period and adopt the peptide urea composite fertilizer 10kg/ mu of embodiment 3 preparations, fruit expanding period to topdress for the second time and adopt embodiment 3 to prepare.
(3) variance analysis is used DPS data handling system computed in software and is got.
(4) result and analysis:
Test-results shows that the per plant number is processed C group (50.3)〉processing A group (40.6)
As seen from Table 1 and Table 2, process C group per mu yield 6234kg, process A group volume increase 409kg, amplification reaches 7.02%.Analysis of variance reaches utmost point conspicuous level (P＜0.01) between processing C group and processing A group.
Brief summary, treatment group C obviously improves the biological character of tomato than processing the A group, can improve number of results and the single fruit weight of individual plant, and can access tomato yield the effect of increasing income.
The manure trial of embodiment 6 peptide urea composite fertilizers of the present invention on corn character and output
Test design:
(1) test is processed: in the consistent situation of base manure, 2 processing are established in test altogether, and processing A is habitual fertile amount; Processing C is the peptide urea composite fertilizer of embodiment 3 preparations, and usage quantity is 2/3 of habits fertilization amount.Adopt randomized block design, triplicate;
(2) fertilizer amount and application process
Process A: base manure: use the 5kg diammonium phosphate for every mu, double superhosphate 20kg/ mu; Topdress: the urea 10kg/ mu that topdresses for the first time after the final singling, the typhon mouth phase urea 10kg/ mu that topdresses for the second time.
Process C: base manure: use the 5kg diammonium phosphate for every mu, double superhosphate 20kg/ mu; Topdress: the peptide urea composite fertilizer 6.7kg/ mu that topdresses for the first time after the final singling and adopt the peptide urea composite fertilizer 6.7kg/ mu of embodiment 3 preparations, typhon mouth phase to topdress for the second time and adopt embodiment 3 to prepare.
(3) variance analysis is used DPS data handling system computed in software and is got.
(4) result and analysis:
Test-results shows that Dan Suichong (g) processes C group (328.6)〉processing A group (296.6); Single fringe grain heavy (g) is processed C group (270)〉processing A group (241); Thousand seed weight (g) is processed C group (400.3)〉processing A group (381).
As seen from Table 3 and Table 4, process C group per mu yield 783.7kg, process A group volume increase 75.3kg, amplification reaches 10.6%.Analysis of variance reaches utmost point conspicuous level (P＜0.01) between processing C group and processing A group.
Brief summary, treatment group C compares the biological character that processing A group is obviously improved corn, can improve single fringe weight, Grain number per spike and thousand seed weight, and can access corn yield the effect of increasing income.
The manure trial of embodiment 7 peptide urea composite fertilizers of the present invention on cotton proterties and output
Test design:
(1) test is processed: in the consistent situation of base manure, 2 processing are established in test altogether, and processing A is habitual fertile amount; Processing C is the peptide urea composite fertilizer of embodiment 3 preparations, and usage quantity is 1/2 of habits fertilization amount.Adopt randomized block design, triplicate;
(2) fertilizer amount and application process
Process A: base manure: use the 20kg diammonium phosphate for every mu, 10kg urea; Topdress: urea 20kg/ mu, Western composite fertilizer (the new allocation and transportation of Guangxi farming chemical fertilizer company limited) 10 kg/ mus; Respectively in the flower bud phase with spend the length of time and divide and topdress for 2 times.
Process C: base manure: use the 20kg diammonium phosphate for every mu, 10kg urea; Topdress: the peptide urea composite fertilizer 10kg/ mu of embodiment 3 preparations, Western composite fertilizer 10 kg/ mus; Respectively in the flower bud phase with spend the length of time and divide and topdress for 2 times.
(5) variance analysis is used DPS data handling system computed in software and is got.
Result and analysis:
Find out from table 5 and table 6, process C and organize the heavy 5.52g of single tinkling of pieces of jade that process A group volume increase 0.11g, ginning outturn improves 0.5% than processing the A group.Analysis of variance reaches utmost point conspicuous level (P＜0.01) between processing C group and processing A group.
Brief summary: treatment group C compares processing A group and obviously improves cotton quality, can improve single tinkling of pieces of jade weight, individual plant bell number, and can access output of cotton the effect of increasing income.
The manure trial of embodiment 8 peptide urea composite fertilizers of the present invention on capsicum proterties and output
Test design:
(1) test is processed: in the consistent situation of base manure, 2 processing are established in test altogether, and processing A is habitual fertile amount; Common fertilising urea 15kg/ mu, processing C is the peptide urea composite fertilizer of embodiment 3 preparations, usage quantity is 2/3 of habits fertilization amount, i.e. 10kg/ mu.Adopt randomized block design, triplicate;
(2) fertilizer amount and application process
Process A: use 9.6kg Repone K for every mu, 15kg urea and 10kg threeflod calcium superphosphate;
Process C: use 9.6kg Repone K for every mu, peptide urea composite fertilizer and the 10kg threeflod calcium superphosphate of 10kg embodiment 3 preparations.
(3) variance analysis is used DPS data handling system computed in software and is got
(4) result and analysis:
As seen from Table 7, process on average heavy 0.43g of C group capsicum, process A group volume increase 0.06g, improve 16.2%.Analysis of variance reaches utmost point conspicuous level (P＜0.01) between processing C group and processing A group.
Brief summary: treatment group C obviously reaches the effect of increasing income than processing the A group.
The manure trial of embodiment 9 peptide urea composite fertilizers of the present invention on Chinese cabbage proterties and output
Test design:
(1) test is processed: in the consistent situation of base manure, 3 processing are established in test altogether, and processing A is habitual fertile amount; Treatments B is the peptide urea composite fertilizer of embodiment 4 preparations, and usage quantity is 2/3 of habits fertilization amount.Processing C is the peptide urea composite fertilizer of embodiment 3 preparations, and usage quantity is 2/3 of habits fertilization amount.Adopt randomized block design, triplicate;
(2) fertilizer amount and application process
Process A: base manure: use 3.4kg Repone K for every mu, 5.2kg urea and 3.5kg threeflod calcium superphosphate; Topdress: urea 2.6kg/ mu, 1.75kg threeflod calcium superphosphate/mu; 3.4kg Repone K/mu.
Treatments B: base manure: use 3.4kg Repone K for every mu, 5.2kg urea and 3.5kg threeflod calcium superphosphate; Topdress: the peptide urea composite fertilizer 1.73kg/ mu of embodiment 4 preparations, 1.75kg threeflod calcium superphosphate/mu; 3.4kg Repone K/mu
Process C: base manure: use 3.4kg Repone K for every mu, 5.2kg urea and 3.5kg threeflod calcium superphosphate; Topdress: the peptide urea composite fertilizer 1.73kg/ mu of embodiment 3 preparations, 1.75kg threeflod calcium superphosphate/mu; 3.4kg Repone K/mu.
(3) variance analysis is used DPS data handling system computed in software and is got
(4) result and analysis:
As seen from Table 8, process on average heavy 15.97g of 3 Chinese cabbages of C group, process A group volume increase 3.54g, improve 28.4%.Analysis of variance reaches utmost point conspicuous level (P＜0.01) between processing C group and processing A group.
As seen from Table 9,3 Chinese cabbages of treatments B group are heavy 14.2g on average, processes A group volume increase 1.77g, improves 14.2%.Analysis of variance reaches conspicuous level (P＜0.05) between treatments B group and processing A group.
Brief summary: treatment group C obviously reaches the effect of increasing income than processing the A group.Treatment group B reaches the effect of increasing income than processing the A group.Treatments B group the effect of increasing income is not as processing the C group.
More than explanation only is illustrative and is not used in and limits the scope of the invention.
SEQ ID NO：1
Met Ala Arg Phe Ser Leu Leu Ser Ser Thr Ala Ser Ala Gln Met Ile Leu Ala Leu Val Ser Leu Pro Leu Ala Gln Ala Ala Pro Ala Pro Gln Gln Lys ThrGln Val Gly Tyr Tyr Arg Met Leu Gly Asp Tyr Phe Glu Val Ala Ala Leu Gly Tyr Gly Tyr Val Asp Pro Ala Ser Gly Leu Lys GlyIle Asp Lys Asp Leu Gln Ser Leu Leu Ala Leu Arg Met Phe
SEQ ID NO：2
Met Val Leu Ser Glu Lys Val Thr Ala Val Asn Ala Leu Ile Asn Thr Asp Gly Asp Asn Leu Val Ile Asp Thr Gly Ala Ala Gln Cys Phe GlyPro Thr Leu Gly Val Val Gln Thr Asn Leu Lys Phe Asp Ala Ser Leu Tyr GlnPro Glu Val Leu Asp Thr Val Leu Thr AlaThr Val Glu Val Pro Gln Ala Glu Ala Glu Phe Trp Leu Leu Asp Glu AlaThr Met Ala Lys Ala Pro Glu Gly Gly Met Phe His Lys Met AlaGln Ala Ala Pro Tyr Asp Ala Lys Met Asn Lys Leu Lys
SEQ ID NO：3
Met Pro TyrLys Pro Thr Gly Glu Leu Leu Pro Gly Val Val Ser Leu Val Ala Ser ProGly His Thr Pro Gly His Thr Ser Tyr Met Leu Phe Lys Ser Gly Val Gly GlnSer Leu Gln Val Trp Gly Ile Leu Leu Asn Ala Val Gln Glu Phe Ala Lys Pro Glu Val Phe Glu Gly Thr Leu Leu His Pro Asp HisAla Cys Gln Val Asn Ala Gln Asp Gly His Ser Pro Ala Tyr Asn
Sequence table
＜110〉Feng Jiancai
＜120〉fertilizer synergism additive and uses thereof
<210> 1
<211> 79
<212> PRT
＜213〉artificial sequence
<400> 1
Met Ala Arg Phe Ser Leu Leu Ser Ser Thr Ala Ser Ala Gln Met Ile
1 5 10 15
Leu Ala Leu Val Ser Leu Pro Leu Ala Gln Ala Ala Pro Ala Pro Gln
20 25 30
Gln Lys Thr Gln Val Gly Tyr Tyr Arg Met Leu Gly Asp Tyr Phe Glu
35 40 45
Val Ala Ala Leu Gly Tyr Gly Tyr Val Asp Pro Ala Ser Gly Leu Lys
50 55 60
Gly Ile Asp Lys Asp Leu Gln Ser Leu Leu Ala Leu Arg Met Phe
65 70 75
<210> 1
<211> 105
<212> PRT
＜213〉artificial sequence
<400> 2
Met Val Leu Ser Glu Lys Val Thr Ala Val Asn Ala Leu Ile Asn Thr
1 5 10 15
Asp Gly Asp Asn Leu Val Ile Asp Thr Gly Ala Ala Gln Cys Phe Gly
20 25 30
Pro Thr Leu Gly Val Val Gln Thr Asn Leu Lys Phe Asp Ala Ser Leu
35 40 45
Tyr Gln Pro Glu Val Leu Asp Thr Val Leu Thr Ala Thr Val Glu Val
50 55 60
Pro Gln Ala Glu Ala Glu Phe Trp Leu Leu Asp Glu Ala Thr Met Ala
65 70 75 80
Lys Ala Pro Glu Gly Gly Met Phe His Lys Met Ala Gln Ala Ala Pro
85 90 95
Tyr Asp Ala Lys Met Asn Lys Leu Lys
100 105
<210> 1
<211> 83
<212> PRT
＜213〉artificial sequence
<400> 3
Met Pro Tyr Lys Pro Thr Gly Glu Leu Leu Pro Gly Val Val Ser Leu
1 5 10 15
Val Ala Ser Pro Gly His Thr Pro Gly His Thr Ser Tyr Met Leu Phe
20 25 30
Lys Ser Gly Val Gly Gln Ser Leu Gln Val Trp Gly Ile Leu Leu Asn
35 40 45
Ala Val Gln Glu Phe Ala Lys Pro Glu Val Phe Glu Gly Thr Leu Leu
50 55 60
His Pro Asp His Ala Cys Gln Val Asn Ala Gln Asp Gly His Ser Pro
65 70 75 80
Ala Tyr Asn
Claims (5)
1. fertilizer synergism additive, its weight ratio prescription is as follows:
2,20 parts of 2, 4-dinitrophenol or its sodium salts, 10 parts of α-naphthaleneacetic acid or its sodium salts, 10 parts of 5 nitroguaiacol or its sodium salts, 15 parts of adjacent nitro tea phenol or its sodium salts, 15 parts of p-NP or its sodium salts, 5 parts of Witco 1298 Soft Acid or its sodium salts, 10 parts of poly aspartic acids, 0.05 part of Triiodobenzoic acid, 3 parts of biological nitrogen fixation bacterium, 6 parts of protein peptide, 4 parts of polyether-tribasic alcohols, wherein said biological nitrogen fixation bacterium is that Wei Nielande vinelandii (Azotobacter vinelandii) and blown-ball Azotobacter (Azotobacter chroococcum) are according to the microbial inoculum of the ratio preparation of 1:1, described protein peptide is by two parts of magnetic Nano protein peptide A, two parts of slowly-releasing protein peptide B, two parts of compositions of protein peptide C
Magnetic Nano protein peptide A wherein, the preparation method of slowly-releasing protein peptide B is as follows:
Protein peptide A is dissolved among the 50%ACN-0.1%TFA, adds Fe
3O
4/ SiO
2Nano material at room temperature behind the vortex 20min, is put into magnet in the bottom of centrifuge tube, and centrifugal 150r/min removes supernatant liquor, repeats twice, uses 0.1MNH
3.H
2O wash-out Fe
3O
4/ SiO
2Nano material obtains magnetic Nano protein peptide A;
Protein peptide B is ground into 200-300 purpose powder, it is stand-by that zeolite powder is broken into 150-260 order zeolite powder, account for and add suitable quantity of water and stirring in the gross weight 10-20% stearic acid, the protein peptide B powder that will contain gross weight 12-15% adds wherein with stirring slowly, rotating speed 50-120rpm, stir 15-30min, adding accounts for gross weight 15-25% zeolite powder again, makes suspension slurry, the centrifugal 5000-10000r/min of room temperature, 30-60min, adjust slurries pH and reach 6.8-8.0, wash 3-5 time, filter or centrifuge dehydration, dry, obtain having the slowly-releasing protein peptide B of slow releasing function;
The aminoacid sequence of wherein said protein peptide A is shown in the SEQ ID NO:1, and the aminoacid sequence of protein peptide B is shown in the SEQ ID NO:2, and the aminoacid sequence of protein peptide C is shown in the SEQ ID NO:3.
2. the using method of a fertilizer synergism additive, it is characterized by fertilizer synergism additive as claimed in claim 1 mixes with the urea raw material in the ratio of the 0.01-5% of urea raw material weight, be processed into peptide urea composite fertilizer, its mixture is processed into particulate state, then is administered to crop or vegetables.
3. the using method of fertilizer synergism additive according to claim 2, the water mixed-shaped yellowly mashed prod doubly measured with the 1-5 of its weight first in 1% ratio of urea raw material weight of fertilizer synergism additive as claimed in claim 1 wherein, with yellow mashed prod and urea raw material stirring mixing, its mixture is processed into particulate state, then is administered to crop or vegetables.
One kind as claimed in claim 1 fertilizer synergism additive improving farm crop and the output of vegetables, the purposes aspect the quality.
5. purposes according to claim 4 is characterized in that, is improving tomato, corn, cotton, capsicum, the output of Chinese cabbage, the purposes aspect the quality.
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| CN 201310014107 CN103044147B (en) | 2013-01-15 | 2013-01-15 | Fertilizer effect enhancing additive and application thereof |
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| CN103044147B true CN103044147B (en) | 2013-10-16 |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103772027B (en) * | 2014-01-16 | 2016-03-02 | 江苏集贤绿色化学科技研究院有限公司 | A kind of volume increase chemical fertilizer for farm crop and preparation method thereof |
| CN103787720B (en) * | 2014-01-16 | 2015-10-21 | 江苏集贤绿色化学科技研究院有限公司 | A kind of safe and harmless chemical fertilizer and preparation method thereof |
| CN103910573A (en) * | 2014-03-26 | 2014-07-09 | 宋苏婷 | Agricultural synergic fertilizer |
| CN104311262A (en) * | 2014-10-02 | 2015-01-28 | 青岛锐志农业技术服务有限公司 | High-efficiency chemical fertilizer synergist and production method thereof |
| CN104478611B (en) * | 2014-12-19 | 2017-10-27 | 中化（烟台）作物营养有限公司 | A kind of Synergistic type a great number of elements water-soluble fertilizer anticaking agent |
| CN105777358A (en) * | 2016-03-25 | 2016-07-20 | 马鞍山中粮生物化学有限公司 | Low-cost efficient auxiliary for chemical fertilizer |
| WO2018049547A1 (en) * | 2016-09-13 | 2018-03-22 | 张勇健 | Fertilizer additive and preparation method therefor |
| CN108094109A (en) * | 2017-12-22 | 2018-06-01 | 广西乙木农业科技有限公司 | A kind of efficient cultivation method of Chinese cymbidium |
| CN112028704A (en) * | 2020-10-06 | 2020-12-04 | 青岛苗旺农业科技有限责任公司 | Slow release fertilizer |
| CN115005032B (en) * | 2022-06-15 | 2023-07-25 | 西藏自治区农牧科学院农业研究所 | Planting method for interplanting common turnip with winter wheat |
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| CN101125779A (en) * | 2007-09-12 | 2008-02-20 | 刘长福 | Liquid fertilizer synergistic additive and its manufacturing and using method |
| CN101638338A (en) * | 2008-07-29 | 2010-02-03 | 冯建材 | Magnetic polypeptide fertilizer synergistic additive and using method thereof |
| CN101372435A (en) * | 2008-10-08 | 2009-02-25 | 丁剑兰 | Bio-bacterium fertilizer synergistic additive and preparation thereof |
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