CN102716213A - Coptis and radix sophorae flavescentis anti-dysenteric particle as well as preparation method and application of coptis and radix sophorae flavescentis anti-dysenteric particle - Google Patents
Coptis and radix sophorae flavescentis anti-dysenteric particle as well as preparation method and application of coptis and radix sophorae flavescentis anti-dysenteric particle Download PDFInfo
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Abstract
The invention discloses a coptis and radix sophorae flavescentis anti-dysenteric particle as well as a preparation method and an application of the coptis and radix sophorae flavescentis anti-dysenteric particle and aims at solving the problems that livestock and poultry can generate medicine resistance on the exiting medicine, and meanwhile, the medicine residue can be caused. The particle is prepared from the following ingredients in parts by weight: 400 parts of coptis, 90 parts of radix sophorae flavescentis, 300 parts of Chinese pulsatilla root, 90 parts of myrobalan and 120 parts of liquorice. The recipe of the coptis and radix sophorae flavescentis anti-dysenteric particle is reasonable, the raw medical material resources are wide, the prepared products are mainly used for treating livestock and poultry dampness-heat of liver and gallbladder, heart and stomach fire, damp and hot dysentery, lingering dysentery, piglet white scour and yellow scour, and has the effects of clearing away heat to moisten dryness, purging fire to remove toxin and relieving diarrhea with astringents. Clinic tests prove that the curative effect is exact. The production process selected by in the preparation method provided by the invention has a short production period, the production cost is low, the operation is easy, in addition, active ingredients of raw medical materials are extracted to the furthest degree, and the curative effect of the produced products is stable. Meanwhile, the preparation selected for the coptis and radix sophorae flavescentis anti-dysenteric particle can adapt to the market requirements and the medication habits, and the transportation and the storage are also very convenient.
Description
Technical field
The present invention relates to field of veterinary, especially connect ginseng dysentery relieving granule, its preparation method and application.
Background technology
Sichuan is animal husbandry big producing province, and its pig industry ranks first in the country, and poultry farming also gets prosperous with every passing day.Sichuan year in 2003 1.97 hundred million of stock raisinies, 14.1 hundred million of poultry, and along with the greatly developing of animal husbandry, in rising trend year by year, to the end of the year 2005, the cattle breeding amount has reached 2.124 hundred million, the home poultry raising amount has reached 16.8 hundred million.But because some common disease, like escherichia coli, Pullorum Disease Salmonella etc., the ubiquity in poultry, fowl seriously restricts the development of poultry industry.The Pullorum Disease Salmonella can cause the chickling morbidity, and laying rate of laying hen descends, even causes higher mortality rate.In the generaI investigation of Sichuan Province's animal epidemic, its positive rate reaches 11.35%.Piglet pujos blancos by escherichia coli cause can cause large quantities of baby pig diarrhoeas usually, become thin, even dead.According to statistics, the sickness rate of Sichuan piglet pujos blancos over the years is all about 10%.Simultaneously, this bacterioid like cattle, horse, rabbit, duck, goose etc., all has same appeal to other domestic animals and fowls.Receive the influence of this type disease, whole animal husbandry loss is huge.
In order to prevent and treat this type disease, the annual needs usually used a large amount of antibacterials, and like quinolone medicine and other various antibiotic medicines such as ofloxacin, enrofloxacins, annual requirement surpasses 200,000,000 yuan of RMB to this quasi drugs in market, Sichuan.In the process of using these medicines; There is two large problems: the first, because the lack of standardization and long-term prescription of domestic animals and fowls has made poultry produce tangible drug resistance; Though dosage is in continuous increase, and curative effect is not significantly improved even constantly decline; The second, this quasi drugs is in practical application, because can not strict restriction withdrawal time, and the overdose of medicine uses, and medicine accumulation is in animal body constantly increased, and causes in animal derived food the drug residue severe overweight.This not only makes the outlet of this based food produce and has a strong impact on, and aspect foreign exchange earning, causes great loss for country; And behind edible this based food of people, to healthy very harmful, it can make the people produce drug resistance, when needs use this quasi drugs, needs excess to take in, and excess drug brings gradation and the infringement that can not answer can for people's organ.Therefore, must reduce, perhaps avoid the use of this quasi drugs, thereby guarantee the health of human body.To the maximum disease of poultry harm, a large amount of antibiotic uses not only aggravate the drug resistance of pathogenic bacterium to digestive system disease, have also increased the residual of livestock products medicines, finally have influence on eater's health the most.Therefore, noresidue, the Chinese medicine preparation treatment poultry digestive system disease of nonhazardous receives culturist's welcome day by day.
Summary of the invention
Goal of the invention of the present invention is: the problem to above-mentioned existence provides a kind of company to join dysentery relieving granule, its preparation method and application.The present invention fills a prescription rationally, and crude drug wide material sources, prepared product cure mainly poultry, fowl dampness-heat in the liver and gallbladder, and heart stomach-fire is contained, damp-heat dysentery, and chronic dysentery, piglet pujos blancos, yellow scours, it has heat clearing and damp drying, eliminating fire and detoxication, the effect of relieving diarrhea with astringents.Clinical trial proves, its determined curative effect.The selected production technology of the present invention is with short production cycle, low production cost, and easy operating, and extracted the effective ingredient of crude drug to greatest extent, the product stable curative effect of production.Simultaneously, the selected dosage form of the present invention can adapt to market demand and medication custom, transports, preserves also more convenient.
To achieve these goals, the present invention adopts following technical scheme:
Connect and to join the dysentery relieving granule, it is characterized in that, form by the feedstock production of following mass parts: 400 parts of Rhizoma Coptidis, 90 parts of Radix Sophorae Flavescentiss, 300 parts of the Radix Pulsatillaes, 90 parts of Fructus Chebulaes, 120 parts in Radix Glycyrrhizae.
The particulate method for preparing of dysentery relieving is joined by above-mentioned company, comprises the steps:
(1) takes by weighing each component respectively by proportioning, Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder;
(2) get Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae, soak 0.2-2h, boil 1-2h to wherein adding Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass 2-6 decocting doubly again, filter, win time filtrating and filtering residue for the first time; Add Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass 2-6 decocting doubly in the filtering residue to the first time again and boil 1-2h, filter, filtrating and filtering residue for the second time for the second time; Add Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass 2-6 decocting doubly in the filtering residue to the second time again and boil 1-2h, filter, filtrating for the third time; First, second, third filtrating is merged, be condensed into thick paste;
(3) in thick paste, add Rhizoma Coptidis powder and appropriate amount of auxiliary materials, mixing is granulated, and drying promptly gets product.
In the said step (1), Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder, crosses 100 mesh sieves.
In the said step (2); Get Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae; The water logging bubble 0.5h that adds 2 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass; Boil 1.5h to the decocting that wherein adds 4 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass again, filter, win time filtrating and filtering residue for the first time; The decocting that adds 3 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass in the filtering residue to the first time again boils 1.5h, filter, filtrating and filtering residue for the second time for the second time; The decocting that adds 3 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass in the filtering residue to the second time again boils 1.5h, filter, filtrating for the third time.
In the said step (2), first, second, third filtrating is merged, concentrate, in the time of 80 ℃, relative density is 1.3 thick paste.
In the said step (3), in thick paste, add Rhizoma Coptidis powder and appropriate amount of auxiliary materials, mixing is granulated, drying, and granulate promptly gets product.
Said adjuvant is a kind of in sucrose, starch, the dextrin.
Said adjuvant is a starch.
In the said step (3), baking temperature is 60 ℃.
The particulate application of dysentery relieving is joined by above-mentioned company, it is characterized in that, presses the animal body restatement, every 1kg medication 1.0g, twice on the one.
The Rhizoma Coptidis nature and flavor are bitter, cold, have pathogenic fire purging, dampness, detoxifcation, parasiticidal function.The Rhizoma Coptidis action range, wherein remarkable and commonly used with antimicrobial and protozoacide effect.In vitro tests proves; Rhizoma Coptidis is effective to various bacteria such as Diplococcus pneumoniae, vibrio cholera, staphylococcus aureus, dysentery bacteriums; Its decoct has good antibacterial action, and behind streptomycin, chloromycetin, oxytetracycline generation drug resistance, contained berberine still has effect.
The Radix Pulsatillae has the function of heat-clearing and toxic substances removing, eliminating pathogenic heat from blood to cure dysentery, and in vitro tests has the obvious suppression effect to staphylococcus aureus, dysentery bacterium, bacillus subtilis, Bacillus typhi, Salmonella etc.
Matrine all has antibacterial action in vivo and in vitro in the Radix Sophorae Flavescentis, and the effect of anti-lamb dysentery Type B clostridieum welchii and anti-sheep escherichia coli is arranged in vivo, and action intensity is suitable with chloromycetin in the body.1% matrine all has the obvious suppression effect to dysentery bacterium, escherichia coli, Bacillus proteus, group B streptococcus and staphylococcus glucose coccus.
The Fructus Chebulae has broad-spectrum antibacterial action; Common pathogen all there is tangible antibacterial activity; The Gram-positive negative bacterium all there is activity, comprises will Hayes, sonne bacillus, bacillus pyocyaneus, staphylococcus aureus, escherichia coli, streptococcus pneumoniae, Bacillus typhi, Bacillus proteus, Hemolytic streptococcus and bacillus typhi murium etc.
Radix Glycyrrhizae is cold and cool for relaxing all medicines, the key medicine of warm performance.As carrying in " book on Chinese herbal medicine is just ": " Radix Glycyrrhizae, flavor are to sweet, and the property that must neutralize has the merit of transferring benefits, so the detoxifcation that poison gets, firm medicine gets its property of sum, and what medicine administered to bring out the cold got helps outside it, put in poison delay its speed ".In addition, Radix Glycyrrhizae also can be eliminated laryngopharynx swelling and pain, ulcer sores.
The product of the present invention's preparation is yellow to the yellowish-brown granule; Bitter in the mouth.Inventor of the present invention adopts Rhizoma Coptidis, Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae five tastes medical material; According to Chinese medicine monarch, minister, help, make; The principle of treating both the principal and secondary aspects of a disease in conjunction with modern medical theory, infects because the dysentery due to the Salmonella to domestic birds and animals; Carry out scientific matching, thereby obtain the present invention.Company's ginseng dysentery relieving granule of the present invention's preparation has heat clearing and damp drying, eliminating fire and detoxication, the effect of relieving diarrhea with astringents.Cure mainly poultry, fowl dampness-heat in the liver and gallbladder, heart stomach-fire is contained, damp-heat dysentery, chronic dysentery, piglet pujos blancos, yellow scours.Clinical trial proves, can treat bacillary dysentery, determined curative effect.Company's ginseng dysentery relieving granule that the present invention prepares, reasonable recipe, selected dosage form adapt to market demand and medication custom, transport, preserve also more convenient; Simultaneously, the crude drug wide material sources, selected production technology is with short production cycle, low production cost, easy operating, and extracted the effective ingredient of crude drug to greatest extent; Its extracorporeal bacteria inhibitor test shows that to escherichia coli, various bacteria such as Salmonella all have the better inhibited effect.
The specific embodiment
Disclosed all characteristics in this description, or the step in disclosed all methods or the process except mutually exclusive characteristic and/or the step, all can make up by any way.
Disclosed arbitrary characteristic in this description is only if special narration all can be replaced by other equivalences or the alternative features with similar purpose.That is, only if special narration, each characteristic is an example in a series of equivalences or the similar characteristics.
Embodiment 1
Take by weighing Rhizoma Coptidis 400g, Radix Sophorae Flavescentis 90g, Radix Pulsatillae 300g, Fructus Chebulae 90g, Radix Glycyrrhizae 120g respectively.Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder, crosses 100 mesh sieves.Get all the other four kinds of medical materials, 0.3h is steeped in the water logging that adds 1.5 times of four kinds of medical material gross masses, boils 2h to the decocting that wherein adds 6 times of four kinds of medical material gross masses again, filters, win time filtrating and the filtering residue first time; The decocting that in first time filtering residue, adds 4 times of four kinds of medical material gross masses again boils 2h, filter, filtrating and filtering residue for the second time for the second time; The decocting that in second time filtering residue, adds 4 times of four kinds of medical material gross masses again boils 2h, filter, filtrating for the third time.First, second, third filtrating is merged, concentrate, in the time of 80 ℃, relative density is 1.3 thick paste.In thick paste, add Rhizoma Coptidis powder and appropriate amount of starch after pulverizing, mixing is granulated, drying, and granulate is processed 1000g, promptly gets product.
Embodiment 2
Take by weighing Rhizoma Coptidis 400g, Radix Sophorae Flavescentis 90g, Radix Pulsatillae 300g, Fructus Chebulae 90g, Radix Glycyrrhizae 120g respectively.Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder, crosses 100 mesh sieves.Get all the other four kinds of medical materials, 0.5h is steeped in the water logging that adds 2 times of four kinds of medical material gross masses, boils 1.5h to the decocting that wherein adds 4 times of four kinds of medical material gross masses again, filters, win time filtrating and the filtering residue first time; The decocting that in first time filtering residue, adds 3 times of four kinds of medical material gross masses again boils 1.5h, filter, filtrating and filtering residue for the second time for the second time; The decocting that in second time filtering residue, adds 3 times of four kinds of medical material gross masses again boils 1.5h, filter, filtrating for the third time.First, second, third filtrating is merged, concentrate, in the time of 80 ℃, relative density is 1.3 thick paste.In thick paste, add Rhizoma Coptidis powder and appropriate amount of starch after pulverizing, mixing is granulated, drying, and granulate is processed 1000g, promptly gets product.
Embodiment 3
Take by weighing Rhizoma Coptidis 400g, Radix Sophorae Flavescentis 90g, Radix Pulsatillae 300g, Fructus Chebulae 90g, Radix Glycyrrhizae 120g respectively.Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder, crosses 100 mesh sieves.Get all the other four kinds of medical materials, 2h is steeped in the water logging that adds 2 times of four kinds of medical material gross masses, boils 1h to the decocting that wherein adds 3 times of four kinds of medical material gross masses again, filters, win time filtrating and the filtering residue first time; The decocting that in first time filtering residue, adds 3 times of four kinds of medical material gross masses again boils 1.5h, filter, filtrating and filtering residue for the second time for the second time; The decocting that in second time filtering residue, adds 3 times of four kinds of medical material gross masses again boils 1.5h, filter, filtrating for the third time.First, second, third filtrating is merged, concentrate, in the time of 80 ℃, relative density is 1.3 thick paste.In thick paste, add Rhizoma Coptidis powder and appropriate amount of starch after pulverizing, mixing is granulated, drying, and granulate is processed 1000g, promptly gets product.
Process conditions are preferred
A, medical material soak time
Ratio in proportioning takes by weighing medical material, Rhizoma Coptidis 4.0g, Radix Sophorae Flavescentis 0.9g, Radix Pulsatillae 3.0g, Fructus Chebulae 0.9g, Radix Glycyrrhizae 1.2g.Medical material is placed the 100ml graduated cylinder, adding distil water 54ml, airtight, place at shady and cool place, regularly observes.The result is as shown in table 1:
Table 1 medical material different time suction situation
It is little to find out that from table 1 the suction situation of medical material after 0.5 hour changes.Therefore confirm that the medical material soak time is 0.5h.
B, extraction process
For finding out optimum extraction process, selected decocting time, amount of water, number of times, four factors of granularity that extraction conditions is investigated.Method and result are following: getting gross weight by prescription is the 300g medical material, with solid content weight in the decocting liquid and the index of total n-butanol extract as judgement.It is as shown in table 2 to choose the test level, and result of the test is as shown in table 3.
Table 2 orthogonal test water-glass
Table 3 result of the test
Through analyzing, drawing best extraction conditions is A
2B
2C
3D
1, promptly add 10 times of water with granule, decoct each 1.5h 3 times.But big production is prone to gelatinizing as if feeding intake with medicated powder when decocting, and selects granule to feed intake.Consider that in conjunction with production cost selecting optimum extraction conditions is A
2B
2C
3D
2
In order to verify the condition of extraction, by above-mentioned condition parallel test 3 times, result such as following table 4:
Table 4 extraction process replica test
C, adjuvant
The present invention contains a large amount of extractum, and needing the adding adjuvant could absorb these extracts becomes granule.The most frequently used adjuvant of granule has sucrose, starch, dextrin, owing in the middle of these article a large amount of extractum is arranged, considers that from production cost selection starch is excipient.Take weight in order to reduce single, Rhizoma Coptidis powder is broken in the fine powder adding extractum to reduce supplementary product consumption in the prescription.The effects the influence of granularity to preparation, finding to be ground into 100 order fine powders has the ability of stronger absorption extractum than 80 order fine powders, and the granular size that makes is more even, attractive in appearance.Dry back granule is spent good fluidity less than 40 angle of repose.
The flour extraction when pulverizing has also been investigated in experiment, is 80.04% (n=3).The flour extraction that Rhizoma Coptidis is described is lower, for ensuring the quality of products, directly uses Rhizoma Coptidis to pulverize the fine powder of back through 100 mesh sieves when feeding intake.
E, baking temperature:
Begin to occur caking more than 80 ℃, occur soft yolk easily, be unfavorable for particulate even drying, though and low temperature can be dried sample, required time increases, and reduces the output of producing, therefore selecting baking temperature is 60 ℃.
Through preferably, the present invention selected an effective component extracting at most, the production cycle is the shortest, minimum, the easy operation of production cost is grasped technology is as the pilot scale production technology.
Pharmacodynamic experiment
1, to the prevention effect of colibacillosis
(1) experiment material
Receive the reagent thing to adopt company's ginseng dysentery relieving granule of embodiment 2 preparations, escherichia coli 2385 bacterial strains are supervised institute available from middle traditional Chinese medicines, 88 of adult SPF mices; Available from the institute of biological products, Sichuan Province; The positive control medicine is yellow old five, is the Chinese medicine compound granule, and 150g/ listens; Support precious Biology Pharmacy Co., Ltd by Guangdong and produce lot number BN20061103.
(2) experimental technique
Mice is divided into 11 groups at random according to body weight, after in SPF isolated rearing cage, quarantining 2 days, by abdominal cavity inoculation escherichia coli 2385 broth cultures (escherichia coli 2385 bacterial strain 24h cultures, 4.5 * 10
9Cfu/ml) 0.2ml.Wherein 9 groups connect with various dose respectively according to table 1 and to join dysentery relieving granule (2.0,1.0 and 0.5 g/kg body weight) and prevent and treat test; Remain one group of positive medicine contrast in 2 groups; In infecting back 1 day with yellow old five 1.0g of positive control medicine/kg body weight filling stomach; Another group uses the equivalent normal saline to irritate stomach for infecting matched group; Each is organized administration time and is 3 days, and duration of test is behavior of observed and recorded mice and morbidity death condition day by day, and strong number/infection number * 100% of living of mice calculates and respectively organizes cure rate when finishing with treatment.
(3) result
Each organizes mice 5h after infection, and spirit all to occur depressed, and rapid breathing, fear of cold squeeze the heap phenomenon, and diarrhoea appears in indivedual mices, and 12h begins to occur dead, and the 24h death toll peaks, and survival Mus symptom when treatment finished in the 3rd day disappears basically.The artificial challenge mice is without all death in the 3rd day after infection of treatment group, and positive drug treatment group cure rate is 87.5%.Concrete outcome is seen table 5.
Table 5 connects joins the cure rate (%) of dysentery relieving granule to escherichia coli artificial challenge mice
Visible from table 5; The company's ginseng dysentery relieving granule that uses various dose all has a tangible prevention effect to escherichia coli fatal dose infecting mouse; Total cure rate is 58.5%, and tangible dose-effect relationship is arranged, and the cure rate of 2.0 g/kg body weight administrations is the highest; The average cure rate on different dosing opportunity is that the cure rate of 70.8%, 0.5 g/kg body weight is 50.0%; Visible from delivery time, infection administering effect the previous day is best, and 3 average cure rates of dosage are 71.8%; The cure rate that infects the back administration is minimum, is 45.8%, and comprehensive above-mentioned data is visible; Best with 2.0 g/kg body weight in the preventive effect that infects the previous day, be 87.5%.
Through experiment showed, that the present invention has tangible prevention effect to the coli-infection mice of fatal dose, best with 2.0 g/kg body weight in the preventive effect of infection administration in preceding 1 day, suitable with the therapeutic effect of positive control medicine " Huang Laowu ".
2, acute toxicity test
(1) experiment material
Receiving the reagent thing to adopt company's ginseng dysentery relieving granule of embodiment 2 preparations, is that unit calculates dosage even to join dysentery relieving particle weight g/kg body weight.
Compound method: be assigned to desired concn with distilled water.
Route of administration: irritate the clothes administration.
Mice (Kunming kind) is the qualified animal of cleaning level, by Animal Experimental Study chamber, Traditional Chinese Medicine Research Institute, Sichuan Province credit number is provided: SCXK (river) 2005-19 number.
(2) experimental situation
Zoopery barrier system zoopery district carries out in the Traditional Chinese Medicine Research Institute, Sichuan Province in this experiment work, and application facility meets SPF (three) grade standard, the application facility quality certification number: No. 100, the real moving pipe word in river.The indoor automatic air condition control room temperature of passing through is at 19 ~ 21 ℃, and relative humidity is controlled at 50～70%.Regulate room air through automatic air interchanger.The Artificial Control fluorescent illumination, every day, 12hr was bright, and 12hr is dark.The laboratory animal sub-cage rearing, the animal per cage is no more than 5, freely drinks water, and conventional complete feed is fed at regular time and quantity, about every afternoon 4:00, mouse feeding 10g/20g b.w..
(3) experimental technique
Medicine preparation: dysentery relieving granule 20g joins in companys of getting, and with distilled water suspendible (irritating stomach pin degree of being through No. 12) gradually, volume is 34ml at last.Be that every 1ml contains to connect and joins dysentery relieving granule 0.588g.
Trial test: select 10 of body weight 18-22g mices for use, the disposable filling clothes of every Mus connect joins dysentery relieving granule suspension 0.8ml/ only (23.5g/kg), observes through 7 days and does not see that mice has any abnormal conditions that none dead mouse takes place.So decision is carried out maximum dosage-feeding mensuration to even joining the dysentery relieving granule.
Maximum dosage-feeding is measured: select 40 of body weight 18 ~ 22g mices for use, ♀ ♂ half and half is divided into 2 groups at random by body weight, each 10 of every group of ♀ ♂.Fasting 6h before the experiment freely drinks water.The disposable filling clothes of first treated animal are even joined dysentery relieving granule suspension 0.4ml/10g behind the 6h; The disposable filling clothes of second treated animal distilled water 0.4ml/10g observed 14 days.Outward appearance, appetite, autonomic activities, secretions, toxic reaction and the death condition etc. of record mice behind medicine are weighed weekly once, judge mice growth promoter situation.
(4) result of the test
The disposable filling clothes of mice are even joined dysentery relieving granule suspension, observed mice appetite, outward appearance, autonomic activities, secretions and body weight gain equal no abnormality seens such as (seeing table 28) through 14 days behind the self administration of medication.Do not see a death, the observation period finish to put to death dissects, perusal, and each main organs such as brain, the heart, liver, spleen, lung, kidney, adrenal gland, gonad is not seen swelling, blood stasis, abnormal phenomena such as hemorrhage.Administration group and control animals are not seen obviously unusual.The result is as shown in table 6:
Table 6 connects the dysentery relieving granule ig acute toxicity test in mice body weight change situation (
± SD) of joining
Even join dysentery relieving granule suspension Cmax (58.8% to mouse gavaging; Can be to irritate the stomach pin for No. 12 through exceeding), maximum volume (0.4ml/10g body weight) once; Observed through 14 days; Discovery mice appetite, outward appearance, autonomic activities, secretions, growth promoter and body weight etc. are not all seen obviously unusual, do not see a dead mouse.Administration group and control animals are not seen obvious abnormal phenomena.Connect that to join the dysentery relieving granule be 23.5g/kg (its median lethal dose(LD 50) must greater than 5g/kg) to the maximum dosage-feeding of mice.The dysentery relieving granule is even joined in prompting should not have cytotoxic drug in true border, and its clinical practice is safer.
Simultaneously, the present inventor has also carried out experiments such as long term toxicity test and clinical experiment respectively.Long term toxicity test proves, continuous 28 days spices 5.0g/kg body weight of feeding connects and joins the dysentery relieving granule, and chicken weightening finish, liver, spleen, bone marrow, kidney are not had obvious influence; Continuous 28 days spices feed 10.0, the 15.0g/kg body weight connects and joins the dysentery relieving granule, and the chicken weightening finish is not had obviously influence; Liver, kidney, spleen, bone marrow there is mild toxicity.This shows that RD 1.0g/kg body weight is clinical safe in utilization nontoxic.
Clinical trial certificate, the present invention all has better curative effect to pig, chicken, NIUXIA dysentery, and total effective rate is 98.3%.Wherein, the most convictive is treatment to the dysentery of pig, several 317 of test sample, and total effective rate 98.1% is compared with the medicine matched group, and the test specimen therapeutic effect is higher than reference substance, and its significant difference (P≤0.05) has statistical significance.Therapeutic effect to chicken is also good, and 100% healing though sample number is few slightly, also has certain cogency.Treatment to cattle also is to be healing entirely, but because of the test sample number has only 5 examples, so this result of the test is not counted in.
The bacillary dysentery treatment domestic animals and fowls bacillary dysentery that disappears is safe and effective.Clinical recommendation using dosage is every 1.0g/kg body weight, and spice is fed at twice, and the 3-5 days course of treatment, seriously ill animal doubles to throw clothes, one day twice, and evident in efficacy the 3-5 days course of treatment, with the diffusing therapeutic equivalence of the positive control medicine Radix Pulsatillae.
Through evidence, the present invention has heat clearing and damp drying, eliminating fire and detoxication, and the effect of relieving diarrhea with astringents, all flavor Chinese medicine share, the secondary infection of control antibacterial, enhancing human body immunity power and effectively prevented spreading of disease.Desirable to the bacillary dysentery therapeutic effect.This medicine is a granule, and clinical use, convenient transportation have higher development to be worth.
The present invention is not limited to the aforesaid specific embodiment.The present invention expands to any new feature or any new combination that discloses in this manual, and the arbitrary new method that discloses or step or any new combination of process.
Claims (10)
1. connect and join the dysentery relieving granule, it is characterized in that, form by the feedstock production of following mass parts: 400 parts of Rhizoma Coptidis, 90 parts of Radix Sophorae Flavescentiss, 300 parts of the Radix Pulsatillaes, 90 parts of Fructus Chebulaes, 120 parts in Radix Glycyrrhizae.
2. join the particulate method for preparing of dysentery relieving according to the said company of claim 1, it is characterized in that, comprise the steps:
(1) takes by weighing each component respectively by proportioning, Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder;
(2) get Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae, soak 0.2-2h, boil 1-2h to wherein adding Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass 2-6 decocting doubly again, filter, win time filtrating and filtering residue for the first time; Add Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass 2-6 decocting doubly in the filtering residue to the first time again and boil 1-2h, filter, filtrating and filtering residue for the second time for the second time; Add Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass 2-6 decocting doubly in the filtering residue to the second time again and boil 1-2h, filter, filtrating for the third time; First, second, third filtrating is merged, be condensed into thick paste;
(3) in thick paste, add Rhizoma Coptidis powder and appropriate amount of auxiliary materials, mixing is granulated, and drying promptly gets product.
3. join the particulate method for preparing of dysentery relieving according to the said company of claim 2, it is characterized in that, in the said step (1), Rhizoma Coptidis powder is broken into Rhizoma Coptidis powder, cross 100 mesh sieves.
4. join the particulate method for preparing of dysentery relieving according to the said company of claim 2; It is characterized in that, in the said step (2), get Radix Sophorae Flavescentis, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae; The water logging bubble 0.5h that adds 2 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass; Boil 1.5h to the decocting that wherein adds 4 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass again, filter, win time filtrating and filtering residue for the first time; The decocting that adds 3 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass in the filtering residue to the first time again boils 1.5h, filter, filtrating and filtering residue for the second time for the second time; The decocting that adds 3 times of Radix Sophorae Flavescentiss, the Radix Pulsatillae, Fructus Chebulae, Radix Glycyrrhizae gross mass in the filtering residue to the second time again boils 1.5h, filter, filtrating for the third time.
5. join the particulate method for preparing of dysentery relieving according to the arbitrary said company of claim 2-4, it is characterized in that, in the said step (2), first, second, third filtrating is merged, concentrate, in the time of 80 ℃, relative density is 1.3 thick paste.
6. join the particulate method for preparing of dysentery relieving according to the said company of claim 2, it is characterized in that, in the said step (3), in thick paste, add Rhizoma Coptidis powder and appropriate amount of auxiliary materials, mixing is granulated, drying, and granulate promptly gets product.
7. join the particulate method for preparing of dysentery relieving according to claim 2-4,6 arbitrary said companies, it is characterized in that, said adjuvant is a kind of in sucrose, starch, the dextrin.
8. join the particulate method for preparing of dysentery relieving according to the said company of claim 7, it is characterized in that, said adjuvant is a starch.
9. join the particulate method for preparing of dysentery relieving according to claim 2-4,6,7 arbitrary said companies, it is characterized in that, in the said step (3), baking temperature is 60 ℃.
10. join the particulate application of dysentery relieving according to the said company of claim 1, it is characterized in that, press the animal body restatement, every 1kg medication 1.0g, twice on the one.
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Application publication date: 20121010 |