CN102696305A

CN102696305A - Sexual propagation method for gastrodia elata

Info

Publication number: CN102696305A
Application number: CN201210185793XA
Authority: CN
Inventors: 赵天炬; 袁书杰
Original assignee: Huangshan University
Current assignee: Huangshan University
Priority date: 2012-06-07
Filing date: 2012-06-07
Publication date: 2012-10-03
Anticipated expiration: 2032-06-07
Also published as: CN102696305B

Abstract

The invention discloses a sexual propagation method for gastrodia elata, which comprises the steps of: steaming weed tree sticks, placing the weed tree sticks on a fungal bed for inoculating Armillaria mellea, soaking fungus sticks which are inoculated with the Armillaria mellea in Armillaria mellea solution, rolling the fungus sticks for a turn in mixture of gastrodia elata seeds and germination strains and finally operating according to a conventional gastrodia elata production method. The sexual propagation method for gastrodia elata has the advantages that the germination rate of the gastrodia elata seeds is high and the yield of white gastrodia elata and rice-sized gastrodia elata is high; and when the weed tree sticks are steamed at high temperature, the goal of sterilization is realized, the Armillaria mellea can be more easily inoculated on the seeds, the problem that many seeds cannot be inoculated with the Armillaria mellea in time can be solved by soaking the fungus sticks in the Armillaria mellea solution, the Armillaria mellea can be more easily inoculated and the seed yield for the sexual propagation of the gastrodia elata is greatly improved.

Description

A kind of rhizoma Gastrodiae sexual reproduction method

Technical field

The present invention relates to rhizoma Gastrodiae breeding field, exactly is a kind of rhizoma Gastrodiae sexual reproduction method.

Background technology

Rhizoma Gastrodiae is a herbaceos perennial, is distributed in national most of areas.Its dry tuber is also claimed rhizoma Gastrodiae, is commonly used simply and famousr and precious Chinese medicine, diseases such as clinical dizzy, the extremity numbness of being used to have a headache, child convulsion, epilepsy, tic, tetanus more.Rhizoma Gastrodiae relies on wild resource in the past always, and after the wild change artificial planting success seventies in 20th century, the artificial planting rhizoma Gastrodiae becomes the staple source at present.

White, the rice fiber crops of general rhizoma Gastrodiae sexual propagation are planted, though its seed self germination rate is low to be had many seeds can not in time connect armillaria mellea can't to grow with germinateing because of receiving, cause the few and instability extremely of output.The germination rate that this method can better improve seeds of Gastrodia elata in time connects the problem of armillaria mellea with solving, thereby improves the seed yield of rhizoma Gastrodiae sexual propagation greatly.

Summary of the invention

The purpose of this invention is to provide a kind of sexual reproduction method of rhizoma Gastrodiae fast and efficiently.

Above-mentioned purpose realizes through following scheme:

A kind of rhizoma Gastrodiae sexual reproduction method is characterized in that:

May further comprise the steps:

(1) the 1-2 month about 0.5 hour, puts bacterium bench grafting armillaria mellea after the taking-up cooling 80-100 ℃ of following boiling with the weedtree rod before seed maturity, gets the bacterium rod that has connected armillaria mellea;

(2) dry after the Fagaceae fallen leaves sterilization that will collect the first year; Subsequent use; Again supermature solid Germination Strain is cut into or rubs into the fragment of 2-4 mm dia, fallen leaves that will dry at last and Germination Strain fragment stir, and weed tree sawdust juice to the water content that adds through high-temperature sterilization is about 60%; Adding seeds of Gastrodia elata fully mixes thoroughly; The Germination Strain material that must contain seeds of Gastrodia elata, per 100 milliliters of glucose that add about 2 grams in the described weed tree sawdust juice, described seeds of Gastrodia elata consumption is 8-10 of every square metre of sowing areas;

The bacterium rod that (3) will connect armillaria mellea takes out after the submergence in liquid armillaria mellea liquid; In containing the Germination Strain material of seeds of Gastrodia elata, roll a week again; Make its bacterium rod periphery can both be stained with the compound of seeds of Gastrodia elata and Germination Strain material; After press the conventional method operation that rhizoma Gastrodiae is produced again, can obtain a large amount of medium and small pockmarks of 0 generation.

Described a kind of rhizoma Gastrodiae sexual reproduction method is characterized in that: described solid Germination Strain refers to osmund or the little mushroom of the stem of noble dendrobium, long about 30 centimeters of described weedtree rod.

Described a kind of rhizoma Gastrodiae sexual reproduction method, it is characterized in that: the preparation method of described weed tree sawdust juice is: getting does not have the weed tree sawdust that goes mouldy, and behind the crushing screening, adds wood chip weight 4-6 water doubly, boils 30-60 minute, gets filtrating after the filtration and promptly gets.

Beneficial effect of the present invention is: the present invention impels gastrodia seed germination rate height, hemp and the numb output of rice high; The weedtree rod can make in sterilization that through thermophilic digestion the bacterium material connects armillaria mellea more easily; The submergence in the liquid armillaria mellea of bacterium rod has been solved the problem that many seeds can not in time connect armillaria mellea; Make to connect armillaria mellea more easily, thereby improve the seed yield of rhizoma Gastrodiae sexual propagation greatly.

Embodiment

Embodiment 1:

1, will grow 30 centimeters weedtree rods with 90 ℃ of following boilings 0.5 hour, put the bacterium bed, dark 20 centimeters of bacterium bed after the taking-up cooling; Wide, length can be respectively one meter, and a bed end puts 5 centimeters rough sand, directly puts 3 wooden sticks; 2 centimeters at interval of each wooden stick terminations; The wooden stick of yaw can be adjacent side by side, and the solid armillaria mellea of walnut size is put in each wooden stick termination, fills up on sand to the excellent face 5 centimeters and get final product to such an extent that connect the bacterium rod of bacterium;

2, dry the fragment that is cut into 2-4 mm dias with the little mushroom of the supermature solid Germination Strain stem of noble dendrobium after the Fagaceae fallen leaves sterilization that will collect the first year; Stir; Adding is through the weed tree sawdust juice of high-temperature sterilization, and per 100 milliliters add 2 gram glucose in the weed tree sawdust juice, to water content be about 60%; Add ripe seeds of Gastrodia elata (6-8 every square metre north fruits) again, fully mix thoroughly the compound of rhizoma Gastrodiae Germination Strain;

The bacterium rod that 3, will connect bacterium rolls a circle in the compound of rhizoma Gastrodiae Germination Strain after the submergence in liquid armillaria mellea liquid, make its bacterium rod periphery can both be stained with the compound of seeds of Gastrodia elata and Germination Strain;

The bacterium rod that 4, will be stained with the compound of seeds of Gastrodia elata and Germination Strain is put into rhizoma Gastrodiae production cellar for storing things; 5 centimeters sands are filled out at the end, cellar for storing things; Put the withered broad-leaved leaf of one deck, rod is not less than 15 centimeters at interval with the termination of rod, and rod is not less than 10 centimeters at interval with rod; The nutrition soil that fills up sand or prepare covers 5-7 centimeters sands above again and gets final product.Note sheltering from heat or light of draining in rainy season and summer high temperature.

Present embodiment can make that seeds of Gastrodia elata output contrast weedtree rod does not have thermophilic digestion, the propagation method with the submergence in liquid armillaria mellea liquid of bacterium rod improves about 15-20%,

Embodiment 2,

A kind of rhizoma Gastrodiae sexual reproduction method,

May further comprise the steps:

(1) before seed maturity February with the weedtree rod 80-90 ℃ of following boiling 30 minutes, put bacterium bench grafting armillaria mellea after taking out cooling, connect the high-quality bacterium rod of armillaria mellea;

(2) dry after the Fagaceae fallen leaves sterilization that will collect the first year; Subsequent use; Again supermature solid Germination Strain is cut into or rubs into the fragment of 2-4 mm dia, fallen leaves that will dry at last and Germination Strain fragment stir, and weed tree sawdust juice to the water content that adds through high-temperature sterilization is about 60%; Adding seeds of Gastrodia elata fully mixes thoroughly; The Germination Strain material that must contain seeds of Gastrodia elata, per 100 milliliters of glucose that add about 2 grams in the described weed tree sawdust juice, described seeds of Gastrodia elata consumption is every square metre of sowing area 8-10 grain;

The high-quality bacterium rod that (3) will connect armillaria mellea takes out after the submergence in liquid armillaria mellea liquid; In containing the Germination Strain material of seeds of Gastrodia elata, roll a week again; Make its bacterium rod periphery can both be stained with the compound of seeds of Gastrodia elata and Germination Strain material; After press the conventional method operation that rhizoma Gastrodiae is produced again, can obtain a large amount of medium and small pockmarks of 0 generation.

Described solid Germination Strain refers to osmund, long 30 centimeters of described weedtree rod.

The preparation method of described weed tree sawdust juice is: getting does not have the weed tree sawdust that goes mouldy, and behind the crushing screening, adds the water of 5 times of wood chip weight, boils 40 minutes, gets filtrating after the filtration and promptly gets.

Present embodiment can make that seeds of Gastrodia elata output contrast weedtree rod does not have thermophilic digestion, the propagation method with the submergence in liquid armillaria mellea liquid of bacterium rod improves 15-20%.

Claims

1. rhizoma Gastrodiae sexual reproduction method is characterized in that:

May further comprise the steps:

2. a kind of rhizoma Gastrodiae sexual reproduction method according to claim 1 is characterized in that: described solid Germination Strain refers to osmund or the little mushroom of the stem of noble dendrobium, long about 30 centimeters of described weedtree rod.

3. a kind of rhizoma Gastrodiae sexual reproduction method according to claim 1; It is characterized in that: the preparation method of described weed tree sawdust juice is: getting does not have the weed tree sawdust that goes mouldy, and behind the crushing screening, adds wood chip weight 4-6 water doubly; Boiled 30-60 minute, and got filtrating after the filtration and promptly get.