CN102686739B - 检测微生物的方法和其套件 - Google Patents
检测微生物的方法和其套件 Download PDFInfo
- Publication number
- CN102686739B CN102686739B CN201080059086.5A CN201080059086A CN102686739B CN 102686739 B CN102686739 B CN 102686739B CN 201080059086 A CN201080059086 A CN 201080059086A CN 102686739 B CN102686739 B CN 102686739B
- Authority
- CN
- China
- Prior art keywords
- detectable product
- fluorescently detectable
- wavelength
- light
- excitation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28888309P | 2009-12-22 | 2009-12-22 | |
| US61/288,883 | 2009-12-22 | ||
| PCT/US2010/060936 WO2011087711A1 (en) | 2009-12-22 | 2010-12-17 | Methods of detecting microorganisms and kits therefore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102686739A CN102686739A (zh) | 2012-09-19 |
| CN102686739B true CN102686739B (zh) | 2014-09-24 |
Family
ID=43855961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201080059086.5A Expired - Fee Related CN102686739B (zh) | 2009-12-22 | 2010-12-17 | 检测微生物的方法和其套件 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US8518664B2 (https=) |
| EP (1) | EP2516666B1 (https=) |
| JP (1) | JP5747043B2 (https=) |
| CN (1) | CN102686739B (https=) |
| BR (1) | BR112012015443B1 (https=) |
| WO (1) | WO2011087711A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5747043B2 (ja) * | 2009-12-22 | 2015-07-08 | スリーエム イノベイティブ プロパティズ カンパニー | 微生物を検出する方法及びそのためのキット |
| FR2980577B1 (fr) * | 2011-09-26 | 2013-09-20 | Biomerieux Sa | Systeme de detection et/ou de quantification in vitro par fluorimetrie |
| CN103343158B (zh) * | 2013-07-20 | 2014-05-14 | 吉林农业大学 | 产气荚膜梭菌检测纸片、制备方法及其应用 |
| US10379125B2 (en) * | 2013-12-27 | 2019-08-13 | Becton, Dickinson And Company | System and method for dynamically calibrating and measuring analyte concentration in diabetes management monitors |
| WO2015157442A1 (en) * | 2014-04-09 | 2015-10-15 | Nch Corporation | System and method for detecting biofilm growth in water systems |
| US20180237821A1 (en) * | 2017-02-23 | 2018-08-23 | Ethicon, Inc. | Apparatus and method to read biological indicator |
| WO2020115661A1 (en) | 2018-12-06 | 2020-06-11 | 3M Innovative Properties Company | FLUORINATED 4' ALKYLUMBELLIFERYL α-D-GLUCOPYRANOSIDES, BIOLOGICAL STERILIZATION INDICATORS INCLUDING THE SAME AND METHODS OF USING THE SAME |
| FR3103900B1 (fr) * | 2019-11-29 | 2024-07-19 | Univ Du Mans | Méthode d'identification rapide de microorganismes par analyse de matrices excitation-émission |
| CN111560408B (zh) * | 2020-02-29 | 2022-11-25 | 浙江工业大学 | 一种基于流动化学酶促在线合成香豆素-3-羧酸糖酯衍生物的方法 |
| CN112679569B (zh) * | 2020-10-22 | 2022-05-27 | 山西大学 | 一种荧光探针及其制备方法和应用 |
| EP4730989A1 (en) * | 2023-06-22 | 2026-04-29 | Sensei AG Holdings, Inc. | Sprayable substances for photosynthesis enhancement |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6372895B1 (en) * | 2000-07-07 | 2002-04-16 | 3M Innovative Properties Company | Fluorogenic compounds |
| WO2004027084A1 (en) * | 2002-09-20 | 2004-04-01 | Queen's University At Kingston | Detection of biological molecules by differential partitioning of enzyme substrates and products |
| CN101203617A (zh) * | 2005-04-20 | 2008-06-18 | 纳诺洛吉克斯公司 | 用于单个微生物的快速检测和鉴定而无需初步培养的装置 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5817598B2 (ja) * | 1979-10-31 | 1983-04-08 | 味の素株式会社 | 微生物の迅速検出方法 |
| US5079144A (en) * | 1982-04-14 | 1992-01-07 | Radiometer Corporate Development Ltd. | Microorganism testing with a hydrolyzable fluorogenic substrate |
| DE3380263D1 (en) * | 1982-04-14 | 1989-08-31 | Radiometer Corporate Dev Limit | Microbiological test processes and apparatus |
| US5354655A (en) * | 1988-03-29 | 1994-10-11 | Biocontrol Systems, Inc. | Method for determining the presence or concentration of a bound enzyme |
| US6387650B1 (en) | 1995-06-07 | 2002-05-14 | Biocontrol Systems, Inc. | Method and composition for detecting bacterial contamination in food products |
| EP1926825A4 (en) * | 2005-09-20 | 2008-09-24 | Univ Arizona State | METHODS FOR DETECTING AND CHARACTERIZING MICROORGANISMS |
| JP5747043B2 (ja) * | 2009-12-22 | 2015-07-08 | スリーエム イノベイティブ プロパティズ カンパニー | 微生物を検出する方法及びそのためのキット |
-
2010
- 2010-12-17 JP JP2012546070A patent/JP5747043B2/ja not_active Expired - Fee Related
- 2010-12-17 CN CN201080059086.5A patent/CN102686739B/zh not_active Expired - Fee Related
- 2010-12-17 BR BR112012015443-5A patent/BR112012015443B1/pt not_active IP Right Cessation
- 2010-12-17 EP EP10798676.2A patent/EP2516666B1/en not_active Not-in-force
- 2010-12-17 WO PCT/US2010/060936 patent/WO2011087711A1/en not_active Ceased
- 2010-12-17 US US13/513,794 patent/US8518664B2/en not_active Expired - Fee Related
-
2013
- 2013-07-31 US US13/955,134 patent/US8802429B2/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6372895B1 (en) * | 2000-07-07 | 2002-04-16 | 3M Innovative Properties Company | Fluorogenic compounds |
| WO2004027084A1 (en) * | 2002-09-20 | 2004-04-01 | Queen's University At Kingston | Detection of biological molecules by differential partitioning of enzyme substrates and products |
| CN101203617A (zh) * | 2005-04-20 | 2008-06-18 | 纳诺洛吉克斯公司 | 用于单个微生物的快速检测和鉴定而无需初步培养的装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2013514809A (ja) | 2013-05-02 |
| EP2516666B1 (en) | 2016-04-20 |
| BR112012015443B1 (pt) | 2019-07-02 |
| JP5747043B2 (ja) | 2015-07-08 |
| EP2516666A1 (en) | 2012-10-31 |
| WO2011087711A1 (en) | 2011-07-21 |
| CN102686739A (zh) | 2012-09-19 |
| US8518664B2 (en) | 2013-08-27 |
| US20130316443A1 (en) | 2013-11-28 |
| BR112012015443A2 (pt) | 2016-03-15 |
| US20120252049A1 (en) | 2012-10-04 |
| US8802429B2 (en) | 2014-08-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102686739B (zh) | 检测微生物的方法和其套件 | |
| AU712226B2 (en) | Method and composition for detecting bacterial contamination in food products | |
| US20130040328A1 (en) | Assay for phytol-free chlorophyll derivatives | |
| KR20200052284A (ko) | 탄수화물 센서 | |
| JPH0472517B2 (https=) | ||
| FR2926563A1 (fr) | Procede de detection et/ou d'identification de clostridium difficile | |
| EP2689027A1 (fr) | Detection de bacteries presentant une resistance enzymatique aux carbapenemes | |
| FR2950363A1 (fr) | Procede d'identification de bacteries du groupe bacillus cereus | |
| EP2331702A2 (fr) | Milieu de culture permettant de differencier staphylococcus aureus des staphylococcus a coagulase negative | |
| EP2981619B1 (fr) | Utilisation d'au moins un substrat de phosphatase chromogène et/ou fluorogène pour la détection et/ou le dénombrement d'entérobactéries dans un échantillon | |
| Branco et al. | An important step forward for the future development of an easy and fast procedure for identifying the most dangerous wine spoilage yeast, Dekkera bruxellensis, in wine environment | |
| JP5189722B2 (ja) | サンプル中の標的微生物検出のための組成物および方法 | |
| US10351896B2 (en) | Use of at least one substrate of carboxylesterase and/or triacylglycerol lipase for detecting bacteria of the group Bacillus cereus | |
| JP2010193815A (ja) | 大腸菌群の検出方法 | |
| JP5823390B2 (ja) | 新規ニトロレダクターゼ酵素基質 | |
| EP2804954A1 (fr) | Detection in vitro de microorganismes presentant une activite azoreductase | |
| FR2933104A1 (fr) | Milieu de culture comportant un compose inhibiteur ou retardateur de germination de spores | |
| JP5730305B2 (ja) | 新規ニトロレダクターゼ酵素基質 | |
| EP2670858B1 (fr) | Milieu de culture de microorganismes comprenant l'acide para-aminobenzoique comme agent selectif | |
| JP2013081424A (ja) | 蛋白質を含む試料中の微生物の生菌測定方法 | |
| JP2024522705A (ja) | 固体増殖培地に蛍光色素を直接添加することによって微生物を検出する方法 | |
| JP2013500039A (ja) | 新規ニトロレダクターゼ酵素基質 | |
| JP2024541849A (ja) | 微生物を検出する方法 | |
| CN119738393A (zh) | 一种基于二氧化锰纳米片响应的荧光传感器在检测大肠杆菌中的应用 | |
| FR2948686A1 (fr) | Nouveaux substrats de peptidase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140924 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |